pycom06g14850

zinc ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0016329
Physical Location & Seq
Reverse (-)
19483494 .. 19484613
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGCAAGAGATTAAATACGCCATTACGATTACGTCCAAGACAATCTCTCCCCTATTATCTTTTTGCCGCCCAAGTGTGAACGTCTCTGAATCATCTTCTCCAACTTCATTCTTCTCTGACCGCTCCTGTGGAGCGATGGCCTCCACCGCGGTCATCGACGTCCGGTATTTCGGAACCTCTAAGGTTAGAGCCGGCGGCAGAGTGAGGCATCTCAGTGCTCGTGTTGATAACAATGGCGAACTGGATCTCGACATGGGAGCATTGCGGAGAGAAATTCGCAGCCTTTGTGCCCTCCCACCCGCTGCTGACATTACTCTGACGTACATTGATGAAAACTACGATGTCGTTACTCTTGTGGACGATGATGATCTGCGGGAAGCAATGAGGCAGCGTCTGGAGCCCTTCAGGATCGATGTGCTGATGAACGATGACAAAGAAGACTGCGGCAAAAACTCAAGCTCCGGAAAATGTCACGTGACGGATGCTGTAAATGCAACAAGTGATGTGAGTAATAGCAATGTGTTTGATGAATTCCATCTGGCAAATTATGATCACCCTGTTAGTCCTAGGGTTAAACAATCTGAAGACGAATGTGGTATGATGTGCGACGCAGGCATTCGCTGTCGCGCCTGCAATGACAAGGTCGCGAAACTGTGGTTAGCGGCGTTGGCCCTCGTAGTGTCGGTCCGGTTATTATCCCGGCGACATGGATCGGATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT MIB_HERC2 Neuralized ZZ zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

241

Amino Acids

26.46

Weight (kDa)

5.41

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 61 - 130 3.1e-08 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016329)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06081
malus_domestica MD06G1166000.v1.1
prunus_persica Prupe.5G165900_v2.0.a1
pyrus_communis pycom06g14850
rosa_chinensis RchiOBHm_Chr7g0192771
rosa_laevigata RLG00000004330
rosa_multiflora Rmu_sc0002040.1_g000018
rosa_roxburghii Rroxscaffold_3G00262700
rosa_rugosa Rorug07G0004500 Rorug07G0004500
rosa_samantha Rh7AG129600 Rh7BG130900 Rh7CG134300 Rh7DG131800
rosa_wichuraiana Rw7G011090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 123
AccII CGCG 3 cut(s) 149, 627, 647
AccIII TCCGGA 1 cut(s) 461
AclWI GGATC 3 cut(s) 252, 416, 718
AcsI RAATTY 2 cut(s) 273, 530
AcuI CTGAAG 2 cut(s) 388, 603
AcvI CACGTG 1 cut(s) 475
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 323
AluBI AGCT 1 cut(s) 459
AluI AGCT 1 cut(s) 459
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 3 cut(s) 252, 416, 718
AlwNI CAGNNNCTG 1 cut(s) 394
Aor13HI TCCGGA 1 cut(s) 461
AoxI GGCC 2 cut(s) 138, 669
ApeKI GCWGC 3 cut(s) 279, 302, 388
ApoI RAATTY 2 cut(s) 273, 530
AspA2I CCTAGG 1 cut(s) 566
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 2 cut(s) 670, 685
AsuC2I CCSGG 1 cut(s) 700
AsuHPI GGTGA 1 cut(s) 545
AvaII GGWCC 1 cut(s) 685
AvrII CCTAGG 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 292
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 219
BbrPI CACGTG 1 cut(s) 475
BbsI GAAGAC 2 cut(s) 444, 591
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 3 cut(s) 289, 291, 400
BccI CCATC 2 cut(s) 130, 543
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 1 cut(s) 700
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 1 cut(s) 567
BisI GCNGC 7 cut(s) 67, 196, 280, 303, 389, 445, 663
BlnI CCTAGG 1 cut(s) 566
BlsI GCNGC 7 cut(s) 68, 197, 281, 304, 390, 446, 664
Bme1390I CCNGG 1 cut(s) 700
Bme18I GGWCC 1 cut(s) 685
BmgT120I GGNCC 2 cut(s) 670, 685
BmiI GGNNCC 2 cut(s) 175, 399
BmrFI CCNGG 1 cut(s) 700
BmsI GCATC 2 cut(s) 217, 472
BpiI GAAGAC 2 cut(s) 444, 591
BpmI CTGGAG 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 439
BpuMI CCSGG 1 cut(s) 700
Bsa29I ATCGAT 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 475
BsaBI GATNNNNATC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 147, 566
BsaWI WCCGGW 3 cut(s) 162, 461, 687
BsaXI ACNNNNNCTCC 4 cut(s) 31, 61, 443, 473
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 246
Bse3DI GCAATG 4 cut(s) 260, 387, 523, 640
Bse8I GATNNNNATC 1 cut(s) 366
BseAI TCCGGA 1 cut(s) 461
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 2 cut(s) 147, 566
BseGI GGATG 1 cut(s) 487
BseJI GATNNNNATC 1 cut(s) 366
BseMI GCAATG 4 cut(s) 260, 387, 523, 640
BseMII CTCAG 1 cut(s) 226
BseNI ACTGG 1 cut(s) 246
BseSI GKGCMC 1 cut(s) 292
BseXI GCAGC 3 cut(s) 289, 291, 400
Bsh1236I CGCG 3 cut(s) 149, 627, 647
BshFI GGCC 2 cut(s) 140, 671
BshVI ATCGAT 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 220
BsiSI CCGG 5 cut(s) 163, 192, 462, 688, 700
BsmAI GTCTC 1 cut(s) 88
BsmBI CGTCTC 1 cut(s) 88
BsmI GAATGC 1 cut(s) 615
BsnI GGCC 2 cut(s) 140, 671
Bsp1286I GDGCHC 3 cut(s) 220, 292, 402
Bsp13I TCCGGA 1 cut(s) 461
Bsp143I GATC 5 cut(s) 244, 367, 408, 550, 710
Bsp68I TCGCGA 1 cut(s) 647
BspANI GGCC 2 cut(s) 140, 671
BspCNI CTCAG 1 cut(s) 225
BspDI ATCGAT 1 cut(s) 411
BspEI TCCGGA 1 cut(s) 461
BspFNI CGCG 3 cut(s) 149, 627, 647
BspLI GGNNCC 2 cut(s) 175, 399
BspPI GGATC 3 cut(s) 252, 416, 718
BsrBI CCGCTC 1 cut(s) 123
BsrDI GCAATG 4 cut(s) 260, 387, 523, 640
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 1 cut(s) 246
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 147, 566
BssMI GATC 5 cut(s) 244, 367, 408, 550, 710
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 566
Bst2BI CACGAG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 654
BstACI GRCGYC 1 cut(s) 159
BstBAI YACGTR 1 cut(s) 475
BstC8I GCNNGC 3 cut(s) 193, 613, 631
BstDEI CTNAG 2 cut(s) 180, 212
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 487
BstFNI CGCG 3 cut(s) 149, 627, 647
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 5 cut(s) 247, 370, 411, 553, 713
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 5 cut(s) 244, 367, 408, 550, 710
BstMWI GCNNNNNNNGC 5 cut(s) 146, 397, 491, 612, 668
BstSCI CCNGG 1 cut(s) 698
BstSLI GKGCMC 1 cut(s) 292
BstUI CGCG 3 cut(s) 149, 627, 647
BstV1I GCAGC 3 cut(s) 289, 291, 400
BstV2I GAAGAC 2 cut(s) 444, 591
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 2 cut(s) 140, 671
BsuTUI ATCGAT 1 cut(s) 411
BtgI CCRYGG 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 149
BtsCI GGATG 1 cut(s) 487
BtsIMutI CAGTG 1 cut(s) 220
BtuMI TCGCGA 1 cut(s) 647
Cac8I GCNNGC 3 cut(s) 193, 613, 631
CaiI CAGNNNCTG 1 cut(s) 394
CfoI GCGC 1 cut(s) 629
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 2 cut(s) 670, 685
Cfr42I CCGCGG 1 cut(s) 150
ClaI ATCGAT 1 cut(s) 411
CpoI CGGWCCG 1 cut(s) 685
CseI GACGC 2 cut(s) 380, 617
Csp6I GTAC 1 cut(s) 322
CspI CGGWCCG 1 cut(s) 685
CviAII CATG 2 cut(s) 253, 707
CviJI RGCY 6 cut(s) 140, 191, 282, 400, 459, 671
CviKI_1 RGCY 6 cut(s) 140, 191, 282, 400, 459, 671
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 2 cut(s) 180, 212
DpnI GATC 5 cut(s) 246, 369, 410, 552, 712
DpnII GATC 5 cut(s) 244, 367, 408, 550, 710
Eco130I CCWWGG 1 cut(s) 566
Eco24I GRGCYC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 685
Eco57I CTGAAG 2 cut(s) 388, 603
Eco72I CACGTG 1 cut(s) 475
EcoRI GAATTC 1 cut(s) 530
EcoT14I CCWWGG 1 cut(s) 566
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 566
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 2 cut(s) 256, 710
FaiI YATR 4 cut(s) 254, 549, 599, 708
FatI CATG 2 cut(s) 252, 706
FauI CCCGC 2 cut(s) 307, 366
FbaI TGATCA 1 cut(s) 550
Fnu4HI GCNGC 7 cut(s) 67, 196, 280, 303, 389, 445, 663
FokI GGATG 1 cut(s) 494
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 7 cut(s) 67, 196, 280, 303, 389, 445, 663
FspBI CTAG 1 cut(s) 567
GlaI GCGC 1 cut(s) 628
GluI GCNGC 7 cut(s) 67, 196, 280, 303, 389, 445, 663
GsuI CTGGAG 1 cut(s) 416
HaeIII GGCC 2 cut(s) 140, 671
HapII CCGG 5 cut(s) 163, 192, 462, 688, 700
HgaI GACGC 2 cut(s) 380, 617
HhaI GCGC 1 cut(s) 629
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 2 cut(s) 256, 710
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HinfI GANTC 1 cut(s) 89
HpaII CCGG 5 cut(s) 163, 192, 462, 688, 700
HphI GGTGA 1 cut(s) 545
Hpy166II GTNNAC 2 cut(s) 79, 358
Hpy188I TCNGA 6 cut(s) 88, 118, 173, 318, 583, 715
Hpy188III TCNNGA 5 cut(s) 248, 395, 406, 462, 646
Hpy8I GTNNAC 2 cut(s) 79, 358
Hpy99I CGWCG 2 cut(s) 161, 611
HpyAV CCTTC 1 cut(s) 412
HpyCH4III ACNGT 1 cut(s) 654
HpyCH4IV ACGT 5 cut(s) 32, 81, 159, 320, 474
HpyCH4V TGCA 3 cut(s) 4, 494, 633
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 397, 491, 612, 668
HpyF3I CTNAG 2 cut(s) 180, 212
HpySE526I ACGT 5 cut(s) 32, 81, 159, 320, 474
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 2 cut(s) 256, 710
HspAI GCGC 1 cut(s) 627
Kpn2I TCCGGA 1 cut(s) 461
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
Ksp22I TGATCA 1 cut(s) 550
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 5 cut(s) 244, 367, 408, 550, 710
LmnI GCTCC 5 cut(s) 128, 131, 257, 397, 464
Lsp1109I GCAGC 3 cut(s) 289, 291, 400
LweI GCATC 2 cut(s) 217, 472
MaeI CTAG 1 cut(s) 567
MaeII ACGT 5 cut(s) 32, 81, 159, 320, 474
MaeIII GTNAC 3 cut(s) 346, 470, 475
MalI GATC 5 cut(s) 246, 369, 410, 552, 712
MbiI CCGCTC 1 cut(s) 123
MboI GATC 5 cut(s) 244, 367, 408, 550, 710
MboII GAAGA 4 cut(s) 87, 103, 449, 596
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 3 cut(s) 220, 292, 402
MluCI AATT 3 cut(s) 273, 530, 544
MmeI TCCRAC 1 cut(s) 125
MnlI CCTC 6 cut(s) 151, 187, 198, 302, 378, 683
MroI TCCGGA 1 cut(s) 461
MroNI GCCGGC 1 cut(s) 191
MseI TTAA 2 cut(s) 12, 573
MslI CAYNNNNRTG 1 cut(s) 213
MspA1I CMGCKG 2 cut(s) 149, 302
MspI CCGG 5 cut(s) 163, 192, 462, 688, 700
MspR9I CCNGG 1 cut(s) 700
Mva1269I GAATGC 1 cut(s) 615
MvnI CGCG 3 cut(s) 149, 627, 647
MwoI GCNNNNNNNGC 5 cut(s) 146, 397, 491, 612, 668
NaeI GCCGGC 1 cut(s) 193
NciI CCSGG 1 cut(s) 700
NdeII GATC 5 cut(s) 244, 367, 408, 550, 710
NgoMIV GCCGGC 1 cut(s) 191
NlaIII CATG 2 cut(s) 256, 710
NlaIV GGNNCC 2 cut(s) 175, 399
NmuCI GTSAC 2 cut(s) 470, 475
NruI TCGCGA 1 cut(s) 647
PctI GAATGC 1 cut(s) 615
PdiI GCCGGC 1 cut(s) 193
PfeI GAWTC 1 cut(s) 89
PflFI GACNNNGTC 1 cut(s) 641
PkrI GCNGC 7 cut(s) 68, 197, 281, 304, 390, 446, 664
PmaCI CACGTG 1 cut(s) 475
PmlI CACGTG 1 cut(s) 475
Ppu21I YACGTR 1 cut(s) 475
PspCI CACGTG 1 cut(s) 475
PspN4I GGNNCC 2 cut(s) 175, 399
PspPI GGNCC 2 cut(s) 670, 685
PstNI CAGNNNCTG 1 cut(s) 394
PsuI RGATCY 1 cut(s) 244
PsyI GACNNNGTC 1 cut(s) 641
RruI TCGCGA 1 cut(s) 647
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 213
Rsr2I CGGWCCG 1 cut(s) 685
RsrII CGGWCCG 1 cut(s) 685
SacII CCGCGG 1 cut(s) 150
SaqAI TTAA 2 cut(s) 12, 573
SatI GCNGC 7 cut(s) 67, 196, 280, 303, 389, 445, 663
Sau3AI GATC 5 cut(s) 244, 367, 408, 550, 710
Sau96I GGNCC 2 cut(s) 670, 685
ScrFI CCNGG 1 cut(s) 700
SduI GDGCHC 3 cut(s) 220, 292, 402
SetI ASST 9 cut(s) 35, 84, 162, 179, 186, 323, 461, 477, 645
SfaNI GCATC 2 cut(s) 217, 472
Sfr303I CCGCGG 1 cut(s) 150
SgrBI CCGCGG 1 cut(s) 150
SinI GGWCC 1 cut(s) 685
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
Sse9I AATT 3 cut(s) 273, 530, 544
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 1 cut(s) 698
StyI CCWWGG 1 cut(s) 566
TaaI ACNGT 1 cut(s) 654
TaiI ACGT 5 cut(s) 35, 84, 162, 323, 477
TaqI TCGA 3 cut(s) 156, 249, 411
TaqII GACCGA 1 cut(s) 673
TasI AATT 3 cut(s) 273, 530, 544
TauI GCSGC 4 cut(s) 69, 198, 447, 665
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 12, 573
Tru9I TTAA 2 cut(s) 12, 573
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 2 cut(s) 470, 475
TseI GCWGC 3 cut(s) 279, 302, 388
Tsp45I GTSAC 2 cut(s) 470, 475
TspDTI ATGAA 4 cut(s) 96, 345, 437, 543
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 1 cut(s) 220
Tth111I GACNNNGTC 1 cut(s) 641
VpaK11BI GGWCC 1 cut(s) 685
XapI RAATTY 2 cut(s) 273, 530
XmaJI CCTAGG 1 cut(s) 566
XspI CTAG 1 cut(s) 567
ZraI GACGTC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.