pycom06g17960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009177
Physical Location & Seq
Forward (+)
21883987 .. 21886956
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g17960.2

Sequence Viewer

Length: 2235 bp
ATGGACTTGTGGGTTGTTGCAGCAGCTGCAGGCGCTGGATATTTGACTAAGTATTGGCAGAACATATCCAAGAATGGGGACAAAGATGGCTTGTTAAATTTGCCTTTTGGGGATGCAAATCTTGACAAATCTGAATCCTCAAGGCATCCTTTACACAGACTAGGACACAGAAAGAACGTGGGTGATGATGTTTCTACTGATAGAAAAAAGGCTTCAGATGCTTACTTGTTAGAAAGTTCTTGTGGGACAGAAGTGGCTTCTACTAGTGGGTCTGATGGTGAAAAGCTGGGAGACTTGGTTAAGTACAAGGATTGCAATGTACTTTCTTTATCACACATGTCACCGGTATACTCGGGAAATGAAAATGAGAATGGGGATGGGGCTTGGATAAGTCATGATATTGATGACAGTACTGGTGCCACATTGCCTAAATCTTCGAGTGGAGAAAGGGGTTCTTATCATGTTCCTCTAAAAAACAAAAGTTCTATTAGAACTAAGCGTTCATATGGGCATGTGATTAAGCCAGTAAATTCCTTAGAGAGTTGCCTTGTGGCTCAGTTATATAAAGAGCGTGCTGAAGAGGAACATGTGCTTACCCTTCCATCACCATCTAGTCCAAGTATGAGACCATTGTTTGTGACTGATGGAAACCGAATTATCAGCAAAGCAAGTGGGGGTCATTTCAGGTCTCAGATTGGAATCGAGGAAAATAAGCTGCATAAGGAAGCCTACTTGGAAACTAATGAAAATGCATGTGGGGTTCCTCCAGAACCAAAACTTGGTTATTTGGATCTGCCCAAGAAGATGACAATTAAGACAGGAAAGGGGTGGCATGGAAAATTGAGCAGTGCCAGTAAATCGGTCAATGGAAAACACTTACATTCTGAAGGTTCACTGGATGGAGCAGTTCTTCTCTGTCTTGGGATTTCTATTGGCATAATATCTTCTTTGATAGCAAATAAAAGAGAAGTTGACAAGTTGAAAAATTTGTTGAAGCAGACTGAGAACTTGGTTCAGGATCTAGCGGAGGAACTCCAAATGAAAGATTCAGTAACAATGAAGGAAATAGCTAATGAGAATTATGGTGCACAAGATACTTGTGACAGCTCCGTTTTTGATAAGGCACCAAATTCTTTTTCTACTGAACAGAATATGGACAAATATGATGGTAAAAATTCATATAACTGGAAAGCAGAAGAGAGTTCAGAGTCGATGAGTAAAATAGAAGCAGAGCTTGAAGCTGAACTTGAGAGGTTGGGGTTAAACATGAACACTTCTGTTCCGGAGAGAAGATCGATTGATGTTGCTGATCTTGACACAGACTTAATGGCAGATTTTGCTCAAGGCGAGCTGAGAGCTGACTTGGTTGGTGGGCTATCTGTTGCCCAATCCATGTCAAATGAAGAGGCAAGCAGTACCTCTACGGATTATCACTGTGCAAACTATGCAGTTTCTCCCAGAGAGCTTAGCTTGCGTCTGCATGAAGTTATCCAATCAAGACTAGAAGAACGTGTCCAGGAGCTTGAGGTGTCTCTACAAAACAGCCAAAGGAAGGTCCAACTCGCAGAGTTGAAGCACAAGGATTCTTGGAGAGACTTCTCAGACAATCACATACATTCCGGAATCCATGAGATTACAAATTCTGCAGAAAATTGCAAAACCACGGCAGAGCCTCTAGTCATGAACTTATCGGGAGAAGCTTTAGATGCATACAACGAGGCTTACGAAGAGTTGATGAAGATCAGTGAATCAGATGAGGAGGATTCACCTTGTCCGATTTTTGAAACATTCAGTGCTCAGAACCACAGTCAAACTCAAAGTGAAGAGGTGAATGGTTCTGTGGGGAATTTTGCTTTCAGTAAAGAGGAAACCGCAGGTGAAAATTCACCTTGTCAAGTTTTTGAAACATTCAGGCATCAAAGCCCCGGTCAAACTCAAAGTGATGAATTAGATGGTTCTGTGAATCATTTTGATTTCAGTAAAGAGGAAACCGTAGGTGATGATCTCCTTTCATTCATGAGCAAAGCAAGTATCTCGGATGAGGAGCGCATTTCCAGGGTCCAGCATTCAAATAAATCATCTGTCAGTGGGGAGAACAGTGATGATTCCGATGCTGAGATGGAGAAACAGTTGATAAGGCAAATTGTGGAGAAGGCCAAGAAAGGTTCTCCGGTAGTCCTGAATGCACAAAGGTGGTTCCGGTCATTCCGGTCAATTGATGAGAACGAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

745

Amino Acids

82.09

Weight (kDa)

4.98

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1865
Acc36I ACCTGC 1 cut(s) 1865
AccB1I GGYRCC 2 cut(s) 416, 1123
AccB7I CCANNNNNTGG 1 cut(s) 779
AccI GTMKAC 1 cut(s) 348
AccIII TCCGGA 2 cut(s) 1282, 1619
AciI CCGC 2 cut(s) 1025, 1872
AclWI GGATC 2 cut(s) 798, 1026
AcsI RAATTY 8 cut(s) 97, 529, 985, 1129, 1174, 1639, 1846, 1882
AcuI CTGAAG 3 cut(s) 198, 597, 906
AfaI GTAC 4 cut(s) 305, 321, 412, 1417
AfiI CCNNNNNNNGG 3 cut(s) 75, 779, 1552
AflIII ACRYGT 3 cut(s) 336, 586, 1510
AgeI ACCGGT 1 cut(s) 343
AgsI TTSAA 7 cut(s) 982, 994, 1238, 1573, 1784, 1904, 2070
AhlI ACTAGT 1 cut(s) 263
AjnI CCWGG 2 cut(s) 1515, 2054
AjuI GAANNNNNNNTTGG 4 cut(s) 762, 794, 1120, 1152
AleI CACNNNNGTG 1 cut(s) 2191
Alw21I GWGCWC 2 cut(s) 1090, 1798
Alw26I GTCTC 5 cut(s) 285, 619, 693, 1536, 1587
Alw44I GTGCAC 1 cut(s) 1086
AlwI GGATC 2 cut(s) 798, 1026
AlwNI CAGNNNCTG 2 cut(s) 26, 35
Ama87I CYCGRG 1 cut(s) 352
Aor13HI TCCGGA 2 cut(s) 1282, 1619
AoxI GGCC 1 cut(s) 2154
ApaLI GTGCAC 1 cut(s) 1086
ApeKI GCWGC 4 cut(s) 20, 23, 26, 715
ApoI RAATTY 8 cut(s) 97, 529, 985, 1129, 1174, 1639, 1846, 1882
ArsI GACNNNNNNTTYG 2 cut(s) 1912, 1944
AsiGI ACCGGT 1 cut(s) 343
AspLEI GCGC 2 cut(s) 35, 2049
AspS9I GGNCC 2 cut(s) 1555, 2059
AsuC2I CCSGG 1 cut(s) 1926
AsuHPI GGTGA 9 cut(s) 194, 290, 333, 597, 1758, 1840, 1878, 1889, 2009
AvaI CYCGRG 1 cut(s) 352
AvaII GGWCC 2 cut(s) 1555, 2059
BaeGI GKGCMC 1 cut(s) 1090
BanI GGYRCC 2 cut(s) 416, 1123
Bbv12I GWGCWC 2 cut(s) 1090, 1798
BbvI GCAGC 4 cut(s) 13, 32, 35, 702
BceAI ACGGC 1 cut(s) 1680
BcgI CGANNNNNNTGC 2 cut(s) 2016, 2050
BciT130I CCWGG 2 cut(s) 1517, 2056
BcnI CCSGG 1 cut(s) 1926
BcoDI GTCTC 5 cut(s) 285, 619, 693, 1536, 1587
BcuI ACTAGT 1 cut(s) 263
BfaI CTAG 6 cut(s) 161, 264, 612, 1022, 1502, 1676
BfmI CTRYAG 2 cut(s) 27, 1644
BfoI RGCGCY 1 cut(s) 36
BfuAI ACCTGC 1 cut(s) 1865
BisI GCNGC 4 cut(s) 21, 24, 27, 716
BlpI GCTNAGC 1 cut(s) 1466
BlsI GCNGC 4 cut(s) 22, 25, 28, 717
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 3 cut(s) 1517, 1926, 2056
Bme18I GGWCC 2 cut(s) 1555, 2059
BmeT110I CYCGRG 1 cut(s) 352
BmgT120I GGNCC 2 cut(s) 1555, 2059
BmiI GGNNCC 5 cut(s) 418, 762, 1125, 2060, 2198
BmrFI CCNGG 3 cut(s) 1517, 1926, 2056
BmsI GCATC 6 cut(s) 103, 154, 208, 1696, 1924, 2101
BpmI CTGGAG 1 cut(s) 750
Bpu1102I GCTNAGC 1 cut(s) 1466
BpuEI CTTGAG 4 cut(s) 124, 1268, 1326, 1544
BpuMI CCSGG 1 cut(s) 1926
Bsa29I ATCGAT 1 cut(s) 1295
BsaBI GATNNNNATC 3 cut(s) 117, 698, 1739
BsaI GGTCTC 2 cut(s) 619, 693
BsaJI CCNNGG 3 cut(s) 1662, 1924, 2055
BsaWI WCCGGW 6 cut(s) 343, 1282, 1619, 2170, 2199, 2208
BsaXI ACNNNNNCTCC 4 cut(s) 282, 312, 2114, 2144
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 779, 1552
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 5 cut(s) 418, 524, 852, 900, 1190
Bse3DI GCAATG 2 cut(s) 322, 422
Bse8I GATNNNNATC 3 cut(s) 117, 698, 1739
BseAI TCCGGA 2 cut(s) 1282, 1619
BseBI CCWGG 2 cut(s) 1517, 2056
BseCI ATCGAT 1 cut(s) 1295
BseDI CCNNGG 3 cut(s) 1662, 1924, 2055
BseGI GGATG 5 cut(s) 118, 145, 382, 904, 2044
BseJI GATNNNNATC 3 cut(s) 117, 698, 1739
BseLI CCNNNNNNNGG 3 cut(s) 75, 779, 1552
BseMI GCAATG 2 cut(s) 322, 422
BseMII CTCAG 7 cut(s) 569, 704, 993, 1343, 1614, 1811, 2106
BseNI ACTGG 5 cut(s) 418, 524, 852, 900, 1190
BseRI GAGGAG 2 cut(s) 1772, 2057
BseSI GKGCMC 1 cut(s) 1090
BseXI GCAGC 4 cut(s) 13, 32, 35, 702
BseYI CCCAGC 1 cut(s) 286
BshFI GGCC 1 cut(s) 2156
BshNI GGYRCC 2 cut(s) 416, 1123
BshTI ACCGGT 1 cut(s) 343
BshVI ATCGAT 1 cut(s) 1295
BsiHKAI GWGCWC 2 cut(s) 1090, 1798
BsiHKCI CYCGRG 1 cut(s) 352
BsiSI CCGG 7 cut(s) 344, 1283, 1620, 1926, 2171, 2200, 2209
BslFI GGGAC 2 cut(s) 92, 259
BslI CCNNNNNNNGG 3 cut(s) 75, 779, 1552
BsmAI GTCTC 5 cut(s) 285, 619, 693, 1536, 1587
BsmFI GGGAC 2 cut(s) 92, 259
BsmI GAATGC 2 cut(s) 2065, 2188
BsnI GGCC 1 cut(s) 2156
Bso31I GGTCTC 2 cut(s) 619, 693
BsoBI CYCGRG 1 cut(s) 352
Bsp1286I GDGCHC 2 cut(s) 1090, 1798
Bsp13I TCCGGA 2 cut(s) 1282, 1619
Bsp143I GATC 6 cut(s) 790, 1018, 1292, 1309, 1740, 2002
Bsp1720I GCTNAGC 1 cut(s) 1466
BspACI CCGC 2 cut(s) 1025, 1872
BspANI GGCC 1 cut(s) 2156
BspCNI CTCAG 7 cut(s) 568, 703, 994, 1344, 1613, 1810, 2107
BspDI ATCGAT 1 cut(s) 1295
BspEI TCCGGA 2 cut(s) 1282, 1619
BspHI TCATGA 3 cut(s) 394, 1680, 2016
BspLI GGNNCC 5 cut(s) 418, 762, 1125, 2060, 2198
BspMAI CTGCAG 2 cut(s) 31, 1648
BspMI ACCTGC 1 cut(s) 1865
BspPI GGATC 2 cut(s) 798, 1026
BspT107I GGYRCC 2 cut(s) 416, 1123
BspTNI GGTCTC 2 cut(s) 619, 693
BsrDI GCAATG 2 cut(s) 322, 422
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 5 cut(s) 418, 524, 852, 900, 1190
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 3 cut(s) 1662, 1924, 2055
BssMI GATC 6 cut(s) 790, 1018, 1292, 1309, 1740, 2002
BssNAI GTATAC 1 cut(s) 349
Bst1107I GTATAC 1 cut(s) 349
Bst2UI CCWGG 2 cut(s) 1517, 2056
Bst4CI ACNGT 6 cut(s) 410, 1436, 1808, 1993, 2099, 2130
Bst6I CTCTTC 5 cut(s) 573, 1191, 1398, 1722, 1818
BstAPI GCANNNNNTGC 3 cut(s) 26, 1337, 1445
BstC8I GCNNGC 5 cut(s) 31, 573, 1349, 1411, 1472
BstDSI CCRYGG 1 cut(s) 1662
BstF5I GGATG 5 cut(s) 118, 145, 382, 904, 2044
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 2049
BstKTI GATC 6 cut(s) 793, 1021, 1295, 1312, 1743, 2005
BstMAI GTCTC 5 cut(s) 285, 619, 693, 1536, 1587
BstMBI GATC 6 cut(s) 790, 1018, 1292, 1309, 1740, 2002
BstMWI GCNNNNNNNGC 7 cut(s) 26, 32, 218, 1337, 1445, 1471, 1706
BstNI CCWGG 2 cut(s) 1517, 2056
BstNSI RCATGY 4 cut(s) 340, 515, 590, 756
BstSCI CCNGG 3 cut(s) 1515, 1924, 2054
BstSFI CTRYAG 2 cut(s) 27, 1644
BstSLI GKGCMC 1 cut(s) 1090
BstV1I GCAGC 4 cut(s) 13, 32, 35, 702
BstX2I RGATCY 2 cut(s) 790, 1018
BstYI RGATCY 2 cut(s) 790, 1018
BstZ17I GTATAC 1 cut(s) 349
Bsu15I ATCGAT 1 cut(s) 1295
BsuRI GGCC 1 cut(s) 2156
BsuTUI ATCGAT 1 cut(s) 1295
BtgI CCRYGG 1 cut(s) 1662
BtsCI GGATG 5 cut(s) 118, 145, 382, 904, 2044
BtsI GCAGTG 1 cut(s) 853
BtsIMutI CAGTG 7 cut(s) 853, 893, 1432, 1750, 1798, 2092, 2104
BveI ACCTGC 1 cut(s) 1865
Cac8I GCNNGC 5 cut(s) 31, 573, 1349, 1411, 1472
CaiI CAGNNNCTG 2 cut(s) 26, 35
CciI TCATGA 3 cut(s) 394, 1680, 2016
CfoI GCGC 2 cut(s) 35, 2049
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 1555, 2059
ClaI ATCGAT 1 cut(s) 1295
CseI GACGC 1 cut(s) 1463
Csp6I GTAC 4 cut(s) 304, 320, 411, 1416
CspAI ACCGGT 1 cut(s) 343
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviQI GTAC 4 cut(s) 304, 320, 411, 1416
DpnI GATC 6 cut(s) 792, 1020, 1294, 1311, 1742, 2004
DpnII GATC 6 cut(s) 790, 1018, 1292, 1309, 1740, 2002
Eam1104I CTCTTC 5 cut(s) 573, 1191, 1398, 1722, 1818
EarI CTCTTC 5 cut(s) 573, 1191, 1398, 1722, 1818
Eco31I GGTCTC 2 cut(s) 619, 693
Eco47I GGWCC 2 cut(s) 1555, 2059
Eco57I CTGAAG 3 cut(s) 198, 597, 906
Eco88I CYCGRG 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 1515, 2054
EcoT22I ATGCAT 2 cut(s) 754, 1711
FalI AAGNNNNNCTT 8 cut(s) 133, 165, 439, 471, 1218, 1250, 1230, 1262
FaqI GGGAC 2 cut(s) 92, 259
FauNDI CATATG 1 cut(s) 505
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 4 cut(s) 21, 24, 27, 716
FokI GGATG 5 cut(s) 125, 132, 389, 911, 2051
Fsp4HI GCNGC 4 cut(s) 21, 24, 27, 716
FspBI CTAG 6 cut(s) 161, 264, 612, 1022, 1502, 1676
GlaI GCGC 2 cut(s) 34, 2048
GluI GCNGC 4 cut(s) 21, 24, 27, 716
GsaI CCCAGC 1 cut(s) 290
GsuI CTGGAG 1 cut(s) 750
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 1 cut(s) 2156
HapII CCGG 7 cut(s) 344, 1283, 1620, 1926, 2171, 2200, 2209
HgaI GACGC 1 cut(s) 1463
HhaI GCGC 2 cut(s) 35, 2049
Hin6I GCGC 2 cut(s) 33, 2047
HinP1I GCGC 2 cut(s) 33, 2047
HincII GTYRAC 1 cut(s) 973
HindII GTYRAC 1 cut(s) 973
HindIII AAGCTT 1 cut(s) 1698
HpaII CCGG 7 cut(s) 344, 1283, 1620, 1926, 2171, 2200, 2209
HphI GGTGA 9 cut(s) 194, 290, 333, 597, 1758, 1840, 1878, 1889, 2009
Hpy166II GTNNAC 4 cut(s) 349, 893, 973, 1088
Hpy8I GTNNAC 4 cut(s) 349, 893, 973, 1088
HpyAV CCTTC 5 cut(s) 608, 881, 1054, 1546, 2146
HpyCH4III ACNGT 6 cut(s) 410, 1436, 1808, 1993, 2099, 2130
HpyCH4IV ACGT 2 cut(s) 177, 1510
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 32, 218, 1337, 1445, 1471, 1706
HpySE526I ACGT 2 cut(s) 177, 1510
HspAI GCGC 2 cut(s) 33, 2047
Kpn2I TCCGGA 2 cut(s) 1282, 1619
Kzo9I GATC 6 cut(s) 790, 1018, 1292, 1309, 1740, 2002
LmnI GCTCC 4 cut(s) 902, 1112, 1519, 2044
Lsp1109I GCAGC 4 cut(s) 13, 32, 35, 702
LweI GCATC 6 cut(s) 103, 154, 208, 1696, 1924, 2101
MaeI CTAG 6 cut(s) 161, 264, 612, 1022, 1502, 1676
MaeII ACGT 2 cut(s) 177, 1510
MaeIII GTNAC 4 cut(s) 339, 637, 1051, 1100
MalI GATC 6 cut(s) 792, 1020, 1294, 1311, 1742, 2004
MboI GATC 6 cut(s) 790, 1018, 1292, 1309, 1740, 2002
MfeI CAATTG 1 cut(s) 2214
MflI RGATCY 2 cut(s) 790, 1018
MhlI GDGCHC 2 cut(s) 1090, 1798
MlyI GAGTC 1 cut(s) 1217
MmeI TCCRAC 1 cut(s) 1582
Mph1103I ATGCAT 2 cut(s) 754, 1711
MroI TCCGGA 2 cut(s) 1282, 1619
MseI TTAA 6 cut(s) 95, 300, 519, 813, 1262, 1325
MslI CAYNNNNRTG 1 cut(s) 2191
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 7 cut(s) 344, 1283, 1620, 1926, 2171, 2200, 2209
MspR9I CCNGG 3 cut(s) 1517, 1926, 2056
MunI CAATTG 1 cut(s) 2214
Mva1269I GAATGC 2 cut(s) 2065, 2188
MvaI CCWGG 2 cut(s) 1517, 2056
MwoI GCNNNNNNNGC 7 cut(s) 26, 32, 218, 1337, 1445, 1471, 1706
NciI CCSGG 1 cut(s) 1926
NdeI CATATG 1 cut(s) 505
NdeII GATC 6 cut(s) 790, 1018, 1292, 1309, 1740, 2002
NlaIV GGNNCC 5 cut(s) 418, 762, 1125, 2060, 2198
NmuCI GTSAC 3 cut(s) 339, 637, 1100
NsiI ATGCAT 2 cut(s) 754, 1711
NspI RCATGY 4 cut(s) 340, 515, 590, 756
OliI CACNNNNGTG 1 cut(s) 2191
PagI TCATGA 3 cut(s) 394, 1680, 2016
PaqCI CACCTGC 1 cut(s) 1865
PciI ACATGT 2 cut(s) 336, 586
PcsI WCGNNNNNNNCGW 1 cut(s) 1722
PctI GAATGC 2 cut(s) 2065, 2188
PfeI GAWTC 9 cut(s) 134, 699, 1046, 1583, 1623, 1748, 1763, 1963, 2105
PflMI CCANNNNNTGG 1 cut(s) 779
PfoI TCCNGGA 1 cut(s) 1515
PinAI ACCGGT 1 cut(s) 343
PkrI GCNGC 4 cut(s) 22, 25, 28, 717
PleI GAGTC 1 cut(s) 1216
PpsI GAGTC 1 cut(s) 1216
PscI ACATGT 2 cut(s) 336, 586
Psp6I CCWGG 2 cut(s) 1515, 2054
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 2 cut(s) 1515, 2054
PspN4I GGNNCC 5 cut(s) 418, 762, 1125, 2060, 2198
PspPI GGNCC 2 cut(s) 1555, 2059
PstI CTGCAG 2 cut(s) 31, 1648
PstNI CAGNNNCTG 2 cut(s) 26, 35
PsuI RGATCY 2 cut(s) 790, 1018
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 4 cut(s) 305, 321, 412, 1417
RsaNI GTAC 4 cut(s) 304, 320, 411, 1416
RseI CAYNNNNRTG 1 cut(s) 2191
SaqAI TTAA 6 cut(s) 95, 300, 519, 813, 1262, 1325
SatI GCNGC 4 cut(s) 21, 24, 27, 716
Sau3AI GATC 6 cut(s) 790, 1018, 1292, 1309, 1740, 2002
Sau96I GGNCC 2 cut(s) 1555, 2059
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 1 cut(s) 1217
ScrFI CCNGG 3 cut(s) 1517, 1926, 2056
SduI GDGCHC 2 cut(s) 1090, 1798
SfaNI GCATC 6 cut(s) 103, 154, 208, 1696, 1924, 2101
SfcI CTRYAG 2 cut(s) 27, 1644
SinI GGWCC 2 cut(s) 1555, 2059
SmiMI CAYNNNNRTG 1 cut(s) 2191
SmlI CTYRAG 4 cut(s) 139, 1247, 1341, 1523
SmoI CTYRAG 4 cut(s) 139, 1247, 1341, 1523
SpeI ACTAGT 1 cut(s) 263
SsiI CCGC 2 cut(s) 1025, 1872
SspMI CTAG 6 cut(s) 161, 264, 612, 1022, 1502, 1676
StyD4I CCNGG 3 cut(s) 1515, 1924, 2054
TaaI ACNGT 6 cut(s) 410, 1436, 1808, 1993, 2099, 2130
TaiI ACGT 2 cut(s) 180, 1513
TaqI TCGA 4 cut(s) 437, 702, 1211, 1295
TaqII GACCGA 1 cut(s) 850
TatI WGTACW 3 cut(s) 303, 319, 410
TfiI GAWTC 9 cut(s) 134, 699, 1046, 1583, 1623, 1748, 1763, 1963, 2105
Tru1I TTAA 6 cut(s) 95, 300, 519, 813, 1262, 1325
Tru9I TTAA 6 cut(s) 95, 300, 519, 813, 1262, 1325
TscAI CASTG 7 cut(s) 853, 900, 1439, 1750, 1798, 2092, 2104
TseFI GTSAC 3 cut(s) 339, 637, 1100
TseI GCWGC 4 cut(s) 20, 23, 26, 715
Tsp45I GTSAC 3 cut(s) 339, 637, 1100
TspGWI ACGGA 2 cut(s) 1099, 1439
TspRI CASTG 7 cut(s) 853, 900, 1439, 1750, 1798, 2092, 2104
Van91I CCANNNNNTGG 1 cut(s) 779
VneI GTGCAC 1 cut(s) 1086
VpaK11BI GGWCC 2 cut(s) 1555, 2059
XapI RAATTY 8 cut(s) 97, 529, 985, 1129, 1174, 1639, 1846, 1882
XceI RCATGY 4 cut(s) 340, 515, 590, 756
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 6 cut(s) 161, 264, 612, 1022, 1502, 1676
ZrmI AGTACT 1 cut(s) 412
Zsp2I ATGCAT 2 cut(s) 754, 1711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.