pycom06g18410

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004415
Physical Location & Seq
Reverse (-)
22189808 .. 22194341
4534 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1716 bp
ATGGAGCTTCCGCAGCCTCGGCCGTTCAGAACCGAAGGAAGAAAACCGACGCATGACTTTCTGTCACTCCACGCTCATTCAGTTGCCCAGCAAGATCCAAGACCACCTGCTCAAGGACGGTACCTTGAAACACATGATTTCTTACGACCACTTGAACGAATGGGAAAGCCATGCATTGCTAAGGAAGAAACCTCAGTTGAGATATCAACCGCTGAAAAACAACCGTCTCATGCGGCAGCGCAGCCGACTAATTCTGTGGAGCATATTCTCCCCGGTGGGATTGGGACTTACAGTATAAGTCACATTTCGTATTTTAATCAAAGGGTTCCGAAACCAGAGGGATCGCCGGTGTTTACAGTCGCTCAAACAAGTAGTAACGAGAGAAACGTTGACGAAAACTCAAACTGTAGTTCTCACACCGGAAGTGGATTCACTTTGTGGGAAGAATCTGCAAAGAATAAGGGAAAGAGAGGGAAGGAGAATGTTGTGGGAGAAAGACCCCTGGGTACCGTGAGAGGGCAATGGACCTCTTCGGAGCGGCCTGCGCAGTCATCTTCCAACAATCATCGCAGCTGCTTCAGTTCTCAGTCATCTGGGCAGAAGAACATGAGCTTCATGGAGATGATGAAGTATGCCGCCAAGGGTAGTACCCAGGAAGCCGAACTTGACGATGAAGAAGAGTTTGTTCTAAAAAAAGAGACCTCTACTACTACCACCAATGCCACCACCACATATAAGAGCGATTTGAGGGTAAACGTTGATGTTAAGAGCTCTGATCAGAAGGCTAACACGCCGCGATCCAAACATTCTGCCACCGAGCAGCGAAGAAGAAGCAAAATAAATGACAGATTCCAAATGTTGAGAGAGCTCATTCCTCACAGTGATCAAAAGAGAGATAAGGCATCGTTTTTATTAGGGGTTATCGAATACATTCAGTTTTTACAGGAACGAGTAAACAAGTACGAGGGTCCATACCAAGGATGGAACCATGATCCAGCAAAATTGATGCCTTGGATCAACGATCACAAGCTTAGGGAGAGTTACTGTGATCAATCTCGAGGCATTAATGTGTCTGGTCCTGCATTACCGTTTGCTGCAAAGTTCGATGAGAAACCTACTTCTGTCCTGCCGACAATCCCTGGAAGCTGCGCACAGAACCCTGTAGAATCCGACACAAGTACTGAAACCACCTTCAAAGCCGTGGATCACCACCCTGGAATAACAAGCAAATCGATGCCCTTTCCTTTGTCAATGCAACCGAACTTCTTCGCTCCTGTCAGGAGTAGTGCTGCCGCTGCTGCTGTACCTCCAATTCCACCTAGACTGCCATCTGATGCAGAGAACACATCTCAGCATCGATCTATGTTATGTCAGACGAGATCGTGTGCTCCTGATGGTACTGCCACTAGGGATAAGCTAAAAGAACAGGAGCTGACAATTGAAGGCGGCCGAATTAACATCTCAAGTGCCTACTCCCAAGGGTTATTGAATACGCTGACACAAGCCCTTCAAAGTTCCGGCGTGGATTTGTCACAGGCCAGTATCTCCGTGAAAATCGAGCTAGGGAAGCGAGCAAACAGAAGATCACCTATACCAAAATCCGCGATTAAGGAAAATGAGATTCCGACAAGCACTTTAGGAAAAAAGCACTCTGTAATTGCAAGCAGTGAAGAATCTGAACAAGCCCAAAAGAAACTCAAGACATTCAGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

572

Amino Acids

63.25

Weight (kDa)

9.0

Isoelectric Point (pI)

56.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 266 - 315 6.4e-16 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 115
Acc16I TGCGCA 2 cut(s) 546, 1150
Acc36I ACCTGC 1 cut(s) 115
Acc65I GGTACC 2 cut(s) 120, 506
AccB1I GGYRCC 2 cut(s) 120, 506
AccBSI CCGCTC 1 cut(s) 538
AccII CGCG 2 cut(s) 796, 1604
AciI CCGC 9 cut(s) 11, 210, 233, 538, 636, 794, 1293, 1446, 1602
AclI AACGTT 2 cut(s) 387, 756
AclWI GGATC 6 cut(s) 89, 349, 792, 986, 1022, 1212
AcoI YGGCCR 2 cut(s) 20, 1447
AcuI CTGAAG 1 cut(s) 562
AdeI CACNNNGTG 1 cut(s) 438
AfaI GTAC 7 cut(s) 122, 508, 649, 962, 1180, 1305, 1399
AfiI CCNNNNNNNGG 3 cut(s) 113, 516, 977
AgsI TTSAA 6 cut(s) 128, 155, 1195, 1442, 1489, 1511
AhdI GACNNNNNGTC 1 cut(s) 61
AjnI CCWGG 4 cut(s) 501, 651, 1138, 1213
Alw21I GWGCWC 3 cut(s) 773, 870, 1390
Alw26I GTCTC 2 cut(s) 231, 692
AlwI GGATC 6 cut(s) 89, 349, 792, 986, 1022, 1212
AlwNI CAGNNNCTG 1 cut(s) 1432
Ama87I CYCGRG 1 cut(s) 1056
AoxI GGCC 4 cut(s) 20, 539, 1447, 1536
AseI ATTAAT 1 cut(s) 1065
Asp700I GAANNNNTTC 2 cut(s) 930, 1265
Asp718I GGTACC 2 cut(s) 120, 506
AspLEI GCGC 3 cut(s) 241, 547, 1151
AspS9I GGNCC 3 cut(s) 525, 968, 1076
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 1199, 1578
AvaI CYCGRG 1 cut(s) 1056
AvaII GGWCC 3 cut(s) 525, 968, 1076
BanI GGYRCC 2 cut(s) 120, 506
BanII GRGCYC 2 cut(s) 773, 870
Bbv12I GWGCWC 3 cut(s) 773, 870, 1390
BccI CCATC 3 cut(s) 975, 1336, 1388
BceAI ACGGC 2 cut(s) 7, 1184
BciT130I CCWGG 4 cut(s) 503, 653, 1140, 1215
BclI TGATCA 3 cut(s) 775, 883, 1048
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 231, 692
BfaI CTAG 3 cut(s) 1320, 1407, 1562
BfmI CTRYAG 2 cut(s) 406, 1161
BfuAI ACCTGC 1 cut(s) 115
BmcAI AGTACT 1 cut(s) 1180
Bme1390I CCNGG 5 cut(s) 273, 503, 653, 1140, 1215
Bme18I GGWCC 3 cut(s) 525, 968, 1076
BmeRI GACNNNNNGTC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 1056
BmgT120I GGNCC 3 cut(s) 525, 968, 1076
BmiI GGNNCC 5 cut(s) 122, 327, 508, 969, 986
BmrFI CCNGG 5 cut(s) 273, 503, 653, 1140, 1215
BmsI GCATC 5 cut(s) 911, 996, 1224, 1324, 1363
Bpu10I CCTNAGC 2 cut(s) 180, 1031
BpuEI CTTGAG 3 cut(s) 96, 1447, 1682
BpuMI CCSGG 1 cut(s) 273
Bsa29I ATCGAT 2 cut(s) 1232, 1357
BsaI GGTCTC 1 cut(s) 692
BsaWI WCCGGW 1 cut(s) 419
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 516, 977
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 1539
Bse3DI GCAATG 2 cut(s) 174, 527
BseBI CCWGG 4 cut(s) 503, 653, 1140, 1215
BseCI ATCGAT 2 cut(s) 1232, 1357
BseGI GGATG 1 cut(s) 986
BseLI CCNNNNNNNGG 3 cut(s) 113, 516, 977
BseMI GCAATG 2 cut(s) 174, 527
BseMII CTCAG 3 cut(s) 207, 599, 1364
BseNI ACTGG 1 cut(s) 1539
BseX3I CGGCCG 2 cut(s) 20, 1447
BseYI CCCAGC 1 cut(s) 87
Bsh1236I CGCG 2 cut(s) 796, 1604
Bsh1285I CGRYCG 2 cut(s) 23, 1450
BshFI GGCC 4 cut(s) 22, 541, 1449, 1538
BshNI GGYRCC 2 cut(s) 120, 506
BshVI ATCGAT 2 cut(s) 1232, 1357
BsiEI CGRYCG 2 cut(s) 23, 1450
BsiHKAI GWGCWC 3 cut(s) 773, 870, 1390
BsiHKCI CYCGRG 1 cut(s) 1056
BsiSI CCGG 4 cut(s) 273, 347, 420, 1518
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 3 cut(s) 113, 516, 977
BsmAI GTCTC 2 cut(s) 231, 692
BsmBI CGTCTC 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 4 cut(s) 22, 541, 1449, 1538
Bso31I GGTCTC 1 cut(s) 692
BsoBI CYCGRG 1 cut(s) 1056
Bsp1286I GDGCHC 3 cut(s) 773, 870, 1390
BspACI CCGC 9 cut(s) 11, 210, 233, 538, 636, 794, 1293, 1446, 1602
BspANI GGCC 4 cut(s) 22, 541, 1449, 1538
BspCNI CTCAG 3 cut(s) 206, 598, 1363
BspDI ATCGAT 2 cut(s) 1232, 1357
BspFNI CGCG 2 cut(s) 796, 1604
BspLI GGNNCC 5 cut(s) 122, 327, 508, 969, 986
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 6 cut(s) 89, 349, 792, 986, 1022, 1212
BspT107I GGYRCC 2 cut(s) 120, 506
BspTNI GGTCTC 1 cut(s) 692
BsrBI CCGCTC 1 cut(s) 538
BsrDI GCAATG 2 cut(s) 174, 527
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 1 cut(s) 1539
BssAI RCCGGY 1 cut(s) 346
BssT1I CCWWGG 4 cut(s) 639, 976, 1010, 1477
Bst2UI CCWGG 4 cut(s) 503, 653, 1140, 1215
Bst4CI ACNGT 9 cut(s) 120, 225, 293, 358, 407, 511, 881, 1046, 1089
Bst6I CTCTTC 2 cut(s) 535, 672
BstC8I GCNNGC 3 cut(s) 543, 1572, 1663
BstDEI CTNAG 5 cut(s) 180, 193, 585, 1031, 1350
BstDSI CCRYGG 1 cut(s) 1200
BstENI CCTNNNNNAGG 1 cut(s) 111
BstF5I GGATG 1 cut(s) 986
BstFNI CGCG 2 cut(s) 796, 1604
BstHHI GCGC 3 cut(s) 241, 547, 1151
BstMAI GTCTC 2 cut(s) 231, 692
BstMCI CGRYCG 2 cut(s) 23, 1450
BstMWI GCNNNNNNNGC 6 cut(s) 13, 19, 544, 1295, 1298, 1567
BstNI CCWGG 4 cut(s) 503, 653, 1140, 1215
BstSCI CCNGG 5 cut(s) 271, 501, 651, 1138, 1213
BstSFI CTRYAG 2 cut(s) 406, 1161
BstUI CGCG 2 cut(s) 796, 1604
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BstZI CGGCCG 2 cut(s) 20, 1447
Bsu15I ATCGAT 2 cut(s) 1232, 1357
BsuRI GGCC 4 cut(s) 22, 541, 1449, 1538
BsuTUI ATCGAT 2 cut(s) 1232, 1357
BtgI CCRYGG 1 cut(s) 1200
BtgZI GCGATG 1 cut(s) 551
BtsCI GGATG 1 cut(s) 986
BtsI GCAGTG 1 cut(s) 1672
BtsIMutI CAGTG 2 cut(s) 886, 1672
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 3 cut(s) 543, 1572, 1663
CaiI CAGNNNCTG 1 cut(s) 1432
CfoI GCGC 3 cut(s) 241, 547, 1151
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 3 cut(s) 525, 968, 1076
ClaI ATCGAT 2 cut(s) 1232, 1357
CseI GACGC 1 cut(s) 58
Csp6I GTAC 7 cut(s) 121, 507, 648, 961, 1179, 1304, 1398
CviAII CATG 7 cut(s) 53, 134, 171, 230, 607, 616, 989
CviQI GTAC 7 cut(s) 121, 507, 648, 961, 1179, 1304, 1398
DdeI CTNAG 5 cut(s) 180, 193, 585, 1031, 1350
DraIII CACNNNGTG 1 cut(s) 438
DriI GACNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 2 cut(s) 20, 1447
EagI CGGCCG 2 cut(s) 20, 1447
Eam1104I CTCTTC 2 cut(s) 535, 672
Eam1105I GACNNNNNGTC 1 cut(s) 61
EarI CTCTTC 2 cut(s) 535, 672
Ecl136II GAGCTC 2 cut(s) 771, 868
EclXI CGGCCG 2 cut(s) 20, 1447
Eco130I CCWWGG 4 cut(s) 639, 976, 1010, 1477
Eco24I GRGCYC 2 cut(s) 773, 870
Eco31I GGTCTC 1 cut(s) 692
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 3 cut(s) 525, 968, 1076
Eco52I CGGCCG 2 cut(s) 20, 1447
Eco53kI GAGCTC 2 cut(s) 771, 868
Eco57I CTGAAG 1 cut(s) 562
Eco88I CYCGRG 1 cut(s) 1056
EcoICRI GAGCTC 2 cut(s) 771, 868
EcoNI CCTNNNNNAGG 1 cut(s) 111
EcoRII CCWGG 4 cut(s) 501, 651, 1138, 1213
EcoRV GATATC 1 cut(s) 204
EcoT14I CCWWGG 4 cut(s) 639, 976, 1010, 1477
EcoT22I ATGCAT 1 cut(s) 176
EcoT38I GRGCYC 2 cut(s) 773, 870
ErhI CCWWGG 4 cut(s) 639, 976, 1010, 1477
Esp3I CGTCTC 1 cut(s) 231
FaeI CATG 7 cut(s) 56, 137, 174, 233, 610, 619, 992
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FaqI GGGAC 1 cut(s) 298
FatI CATG 7 cut(s) 52, 133, 170, 229, 606, 615, 988
FbaI TGATCA 3 cut(s) 775, 883, 1048
FokI GGATG 1 cut(s) 993
FriOI GRGCYC 2 cut(s) 773, 870
FspBI CTAG 3 cut(s) 1320, 1407, 1562
FspI TGCGCA 2 cut(s) 546, 1150
GlaI GCGC 3 cut(s) 240, 546, 1150
GsaI CCCAGC 1 cut(s) 91
HaeIII GGCC 4 cut(s) 22, 541, 1449, 1538
HapII CCGG 4 cut(s) 273, 347, 420, 1518
HgaI GACGC 1 cut(s) 58
HhaI GCGC 3 cut(s) 241, 547, 1151
Hin1II CATG 7 cut(s) 56, 137, 174, 233, 610, 619, 992
Hin6I GCGC 3 cut(s) 239, 545, 1149
HinP1I GCGC 3 cut(s) 239, 545, 1149
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HindIII AAGCTT 1 cut(s) 1028
HinfI GANTC 6 cut(s) 429, 446, 849, 1166, 1621, 1673
HpaII CCGG 4 cut(s) 273, 347, 420, 1518
HphI GGTGA 2 cut(s) 1199, 1578
Hpy166II GTNNAC 4 cut(s) 354, 391, 754, 955
Hpy188III TCNNGA 5 cut(s) 1056, 1279, 1391, 1699, 1708
Hpy8I GTNNAC 4 cut(s) 354, 391, 754, 955
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 6 cut(s) 29, 469, 775, 1201, 1436, 1517
HpyCH4III ACNGT 9 cut(s) 120, 225, 293, 358, 407, 511, 881, 1046, 1089
HpyCH4IV ACGT 2 cut(s) 387, 756
HpyCH4V TGCA 7 cut(s) 174, 452, 1082, 1097, 1255, 1337, 1661
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 19, 544, 1295, 1298, 1567
HpyF3I CTNAG 5 cut(s) 180, 193, 585, 1031, 1350
HpySE526I ACGT 2 cut(s) 387, 756
Hsp92II CATG 7 cut(s) 56, 137, 174, 233, 610, 619, 992
HspAI GCGC 3 cut(s) 239, 545, 1149
KpnI GGTACC 2 cut(s) 124, 510
Ksp22I TGATCA 3 cut(s) 775, 883, 1048
LmnI GCTCC 6 cut(s) 4, 259, 535, 1276, 1393, 1429
LweI GCATC 5 cut(s) 911, 996, 1224, 1324, 1363
MaeI CTAG 3 cut(s) 1320, 1407, 1562
MaeII ACGT 2 cut(s) 387, 756
MaeIII GTNAC 5 cut(s) 63, 299, 374, 1040, 1530
MbiI CCGCTC 1 cut(s) 538
MfeI CAATTG 1 cut(s) 1437
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 3 cut(s) 773, 870, 1390
MluCI AATT 6 cut(s) 250, 1001, 1311, 1437, 1452, 1656
MmeI TCCRAC 3 cut(s) 582, 1194, 1649
Mph1103I ATGCAT 1 cut(s) 176
MroXI GAANNNNTTC 2 cut(s) 930, 1265
MseI TTAA 5 cut(s) 315, 765, 1065, 1455, 1608
MslI CAYNNNNRTG 2 cut(s) 620, 1067
MspA1I CMGCKG 3 cut(s) 212, 573, 1295
MspI CCGG 4 cut(s) 273, 347, 420, 1518
MspR9I CCNGG 5 cut(s) 273, 503, 653, 1140, 1215
MunI CAATTG 1 cut(s) 1437
MvaI CCWGG 4 cut(s) 503, 653, 1140, 1215
MvnI CGCG 2 cut(s) 796, 1604
MwoI GCNNNNNNNGC 6 cut(s) 13, 19, 544, 1295, 1298, 1567
NciI CCSGG 1 cut(s) 273
NlaIII CATG 7 cut(s) 56, 137, 174, 233, 610, 619, 992
NlaIV GGNNCC 5 cut(s) 122, 327, 508, 969, 986
NmuCI GTSAC 3 cut(s) 63, 299, 1530
NsbI TGCGCA 2 cut(s) 546, 1150
NsiI ATGCAT 1 cut(s) 176
PaeR7I CTCGAG 1 cut(s) 1056
PaqCI CACCTGC 1 cut(s) 115
PasI CCCWGGG 1 cut(s) 502
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PdmI GAANNNNTTC 2 cut(s) 930, 1265
PfeI GAWTC 6 cut(s) 429, 446, 849, 1166, 1621, 1673
PshBI ATTAAT 1 cut(s) 1065
Psp124BI GAGCTC 2 cut(s) 773, 870
Psp1406I AACGTT 2 cut(s) 387, 756
Psp6I CCWGG 4 cut(s) 501, 651, 1138, 1213
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 4 cut(s) 501, 651, 1138, 1213
PspN4I GGNNCC 5 cut(s) 122, 327, 508, 969, 986
PspPI GGNCC 3 cut(s) 525, 968, 1076
PstNI CAGNNNCTG 1 cut(s) 1432
PsuI RGATCY 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 573
RsaI GTAC 7 cut(s) 122, 508, 649, 962, 1180, 1305, 1399
RsaNI GTAC 7 cut(s) 121, 507, 648, 961, 1179, 1304, 1398
RseI CAYNNNNRTG 2 cut(s) 620, 1067
SacI GAGCTC 2 cut(s) 773, 870
SaqAI TTAA 5 cut(s) 315, 765, 1065, 1455, 1608
Sau96I GGNCC 3 cut(s) 525, 968, 1076
ScaI AGTACT 1 cut(s) 1180
ScrFI CCNGG 5 cut(s) 273, 503, 653, 1140, 1215
SduI GDGCHC 3 cut(s) 773, 870, 1390
SfaNI GCATC 5 cut(s) 911, 996, 1224, 1324, 1363
SfcI CTRYAG 2 cut(s) 406, 1161
Sfr274I CTCGAG 1 cut(s) 1056
SgrAI CRCCGGYG 1 cut(s) 346
SinI GGWCC 3 cut(s) 525, 968, 1076
SlaI CTCGAG 1 cut(s) 1056
SmiMI CAYNNNNRTG 2 cut(s) 620, 1067
SmlI CTYRAG 4 cut(s) 111, 1056, 1462, 1697
SmoI CTYRAG 4 cut(s) 111, 1056, 1462, 1697
Sse9I AATT 6 cut(s) 250, 1001, 1311, 1437, 1452, 1656
SsiI CCGC 9 cut(s) 11, 210, 233, 538, 636, 794, 1293, 1446, 1602
SspMI CTAG 3 cut(s) 1320, 1407, 1562
SstI GAGCTC 2 cut(s) 773, 870
StyD4I CCNGG 5 cut(s) 271, 501, 651, 1138, 1213
StyI CCWWGG 4 cut(s) 639, 976, 1010, 1477
TaaI ACNGT 9 cut(s) 120, 225, 293, 358, 407, 511, 881, 1046, 1089
TaiI ACGT 2 cut(s) 390, 759
TaqI TCGA 6 cut(s) 924, 1057, 1104, 1232, 1357, 1557
TasI AATT 6 cut(s) 250, 1001, 1311, 1437, 1452, 1656
TatI WGTACW 1 cut(s) 1178
TauI GCSGC 6 cut(s) 236, 541, 638, 796, 1295, 1449
TfiI GAWTC 6 cut(s) 429, 446, 849, 1166, 1621, 1673
Tru1I TTAA 5 cut(s) 315, 765, 1065, 1455, 1608
Tru9I TTAA 5 cut(s) 315, 765, 1065, 1455, 1608
TscAI CASTG 2 cut(s) 886, 1672
TseFI GTSAC 3 cut(s) 63, 299, 1530
Tsp45I GTSAC 3 cut(s) 63, 299, 1530
TspDTI ATGAA 3 cut(s) 604, 641, 687
TspGWI ACGGA 1 cut(s) 1537
TspRI CASTG 2 cut(s) 886, 1672
VpaK11BI GGWCC 3 cut(s) 525, 968, 1076
VspI ATTAAT 1 cut(s) 1065
XagI CCTNNNNNAGG 1 cut(s) 111
XcmI CCANNNNNNNNNTGG 1 cut(s) 978
XhoI CTCGAG 1 cut(s) 1056
XmnI GAANNNNTTC 2 cut(s) 930, 1265
XspI CTAG 3 cut(s) 1320, 1407, 1562
ZrmI AGTACT 1 cut(s) 1180
Zsp2I ATGCAT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.