The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom04g05930 pycom04g09980 pycom04g13690 pycom05g15520 pycom07g05540 pycom09g03940 pycom13g03240 pycom16g04970 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 140 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 193 |
| AclWI | GGATC | 2 cut(s) | 357, 423 |
| AcsI | RAATTY | 3 cut(s) | 160, 476, 495 |
| AcuI | CTGAAG | 1 cut(s) | 450 |
| AfaI | GTAC | 1 cut(s) | 440 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 193, 498 |
| AflIII | ACRYGT | 1 cut(s) | 222 |
| AgsI | TTSAA | 4 cut(s) | 30, 165, 170, 482 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 468 |
| AjnI | CCWGG | 1 cut(s) | 214 |
| Alw26I | GTCTC | 3 cut(s) | 111, 248, 521 |
| AlwI | GGATC | 2 cut(s) | 357, 423 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 132, 193 |
| ApoI | RAATTY | 3 cut(s) | 160, 476, 495 |
| AspLEI | GCGC | 2 cut(s) | 509, 518 |
| AspS9I | GGNCC | 1 cut(s) | 129 |
| AsuHPI | GGTGA | 2 cut(s) | 315, 481 |
| AvaII | GGWCC | 1 cut(s) | 129 |
| BauI | CACGAG | 1 cut(s) | 455 |
| BccI | CCATC | 2 cut(s) | 328, 334 |
| BciT130I | CCWGG | 1 cut(s) | 216 |
| BcoDI | GTCTC | 3 cut(s) | 111, 248, 521 |
| BfoI | RGCGCY | 1 cut(s) | 510 |
| BfuAI | ACCTGC | 1 cut(s) | 140 |
| Bme1390I | CCNGG | 1 cut(s) | 216 |
| Bme18I | GGWCC | 1 cut(s) | 129 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 468 |
| BmgT120I | GGNCC | 1 cut(s) | 129 |
| BmrFI | CCNGG | 1 cut(s) | 216 |
| BpuEI | CTTGAG | 1 cut(s) | 343 |
| BsaJI | CCNNGG | 2 cut(s) | 214, 215 |
| BsaWI | WCCGGW | 1 cut(s) | 491 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 193, 498 |
| Bse1I | ACTGG | 1 cut(s) | 428 |
| BseBI | CCWGG | 1 cut(s) | 216 |
| BseDI | CCNNGG | 2 cut(s) | 214, 215 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 193, 498 |
| BseNI | ACTGG | 1 cut(s) | 428 |
| BseYI | CCCAGC | 1 cut(s) | 135 |
| BsgI | GTGCAG | 1 cut(s) | 5 |
| BsiSI | CCGG | 1 cut(s) | 492 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 193, 498 |
| BsmAI | GTCTC | 3 cut(s) | 111, 248, 521 |
| BsmBI | CGTCTC | 1 cut(s) | 521 |
| Bsp143I | GATC | 3 cut(s) | 362, 381, 415 |
| BspMI | ACCTGC | 1 cut(s) | 140 |
| BspPI | GGATC | 2 cut(s) | 357, 423 |
| BsrI | ACTGG | 1 cut(s) | 428 |
| BssECI | CCNNGG | 2 cut(s) | 214, 215 |
| BssMI | GATC | 3 cut(s) | 362, 381, 415 |
| BssSI | CACGAG | 1 cut(s) | 455 |
| Bst2BI | CACGAG | 1 cut(s) | 455 |
| Bst2UI | CCWGG | 1 cut(s) | 216 |
| Bst4CI | ACNGT | 1 cut(s) | 268 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 132 |
| BstC8I | GCNNGC | 1 cut(s) | 195 |
| BstH2I | RGCGCY | 1 cut(s) | 510 |
| BstHHI | GCGC | 2 cut(s) | 509, 518 |
| BstKTI | GATC | 3 cut(s) | 365, 384, 418 |
| BstMAI | GTCTC | 3 cut(s) | 111, 248, 521 |
| BstMBI | GATC | 3 cut(s) | 362, 381, 415 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 132, 513, 515 |
| BstNI | CCWGG | 1 cut(s) | 216 |
| BstNSI | RCATGY | 1 cut(s) | 226 |
| BstSCI | CCNGG | 1 cut(s) | 214 |
| BstX2I | RGATCY | 2 cut(s) | 362, 415 |
| BstYI | RGATCY | 2 cut(s) | 362, 415 |
| BtsI | GCAGTG | 1 cut(s) | 525 |
| BtsIMutI | CAGTG | 2 cut(s) | 273, 525 |
| BveI | ACCTGC | 1 cut(s) | 140 |
| Cac8I | GCNNGC | 1 cut(s) | 195 |
| CaiI | CAGNNNCTG | 2 cut(s) | 132, 193 |
| CfoI | GCGC | 2 cut(s) | 509, 518 |
| Cfr13I | GGNCC | 1 cut(s) | 129 |
| Csp6I | GTAC | 1 cut(s) | 439 |
| CviAII | CATG | 4 cut(s) | 9, 209, 223, 348 |
| CviJI | RGCY | 1 cut(s) | 193 |
| CviKI_1 | RGCY | 1 cut(s) | 193 |
| CviQI | GTAC | 1 cut(s) | 439 |
| DpnI | GATC | 3 cut(s) | 364, 383, 417 |
| DpnII | GATC | 3 cut(s) | 362, 381, 415 |
| DriI | GACNNNNNGTC | 1 cut(s) | 468 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 468 |
| Eco47I | GGWCC | 1 cut(s) | 129 |
| Eco57I | CTGAAG | 1 cut(s) | 450 |
| EcoO109I | RGGNCCY | 1 cut(s) | 129 |
| EcoRI | GAATTC | 1 cut(s) | 495 |
| EcoRII | CCWGG | 1 cut(s) | 214 |
| Esp3I | CGTCTC | 1 cut(s) | 521 |
| FaeI | CATG | 4 cut(s) | 12, 212, 226, 351 |
| FatI | CATG | 4 cut(s) | 8, 208, 222, 347 |
| GlaI | GCGC | 2 cut(s) | 508, 517 |
| GsaI | CCCAGC | 1 cut(s) | 139 |
| HaeII | RGCGCY | 1 cut(s) | 510 |
| HapII | CCGG | 1 cut(s) | 492 |
| HhaI | GCGC | 2 cut(s) | 509, 518 |
| Hin1II | CATG | 4 cut(s) | 12, 212, 226, 351 |
| Hin6I | GCGC | 2 cut(s) | 507, 516 |
| HinP1I | GCGC | 2 cut(s) | 507, 516 |
| HincII | GTYRAC | 2 cut(s) | 80, 276 |
| HindII | GTYRAC | 2 cut(s) | 80, 276 |
| HinfI | GANTC | 2 cut(s) | 308, 406 |
| HpaII | CCGG | 1 cut(s) | 492 |
| HphI | GGTGA | 2 cut(s) | 315, 481 |
| Hpy166II | GTNNAC | 2 cut(s) | 80, 276 |
| Hpy188I | TCNGA | 1 cut(s) | 400 |
| Hpy188III | TCNNGA | 5 cut(s) | 118, 360, 413, 421, 457 |
| Hpy8I | GTNNAC | 2 cut(s) | 80, 276 |
| Hpy99I | CGWCG | 1 cut(s) | 371 |
| HpyAV | CCTTC | 1 cut(s) | 274 |
| HpyCH4III | ACNGT | 1 cut(s) | 268 |
| HpyCH4IV | ACGT | 1 cut(s) | 378 |
| HpyCH4V | TGCA | 4 cut(s) | 12, 22, 75, 208 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 132, 513, 515 |
| HpySE526I | ACGT | 1 cut(s) | 378 |
| Hsp92II | CATG | 4 cut(s) | 12, 212, 226, 351 |
| HspAI | GCGC | 2 cut(s) | 507, 516 |
| Kzo9I | GATC | 3 cut(s) | 362, 381, 415 |
| MaeII | ACGT | 1 cut(s) | 378 |
| MaeIII | GTNAC | 1 cut(s) | 457 |
| MalI | GATC | 3 cut(s) | 364, 383, 417 |
| MboI | GATC | 3 cut(s) | 362, 381, 415 |
| MboII | GAAGA | 2 cut(s) | 90, 401 |
| MflI | RGATCY | 2 cut(s) | 362, 415 |
| MluCI | AATT | 5 cut(s) | 160, 165, 476, 482, 495 |
| MnlI | CCTC | 5 cut(s) | 142, 183, 254, 285, 289 |
| MseI | TTAA | 1 cut(s) | 233 |
| MspI | CCGG | 1 cut(s) | 492 |
| MspR9I | CCNGG | 1 cut(s) | 216 |
| MvaI | CCWGG | 1 cut(s) | 216 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 132, 513, 515 |
| NdeII | GATC | 3 cut(s) | 362, 381, 415 |
| NlaIII | CATG | 4 cut(s) | 12, 212, 226, 351 |
| NmuCI | GTSAC | 1 cut(s) | 457 |
| NspI | RCATGY | 1 cut(s) | 226 |
| PasI | CCCWGGG | 1 cut(s) | 215 |
| PciI | ACATGT | 1 cut(s) | 222 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 375 |
| PfeI | GAWTC | 2 cut(s) | 308, 406 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 193 |
| PpuMI | RGGWCCY | 1 cut(s) | 129 |
| PscI | ACATGT | 1 cut(s) | 222 |
| Psp5II | RGGWCCY | 1 cut(s) | 129 |
| Psp6I | CCWGG | 1 cut(s) | 214 |
| PspFI | CCCAGC | 1 cut(s) | 135 |
| PspGI | CCWGG | 1 cut(s) | 214 |
| PspPI | GGNCC | 1 cut(s) | 129 |
| PspPPI | RGGWCCY | 1 cut(s) | 129 |
| PstNI | CAGNNNCTG | 2 cut(s) | 132, 193 |
| PsuI | RGATCY | 2 cut(s) | 362, 415 |
| RsaI | GTAC | 1 cut(s) | 440 |
| RsaNI | GTAC | 1 cut(s) | 439 |
| SaqAI | TTAA | 1 cut(s) | 233 |
| Sau3AI | GATC | 3 cut(s) | 362, 381, 415 |
| Sau96I | GGNCC | 1 cut(s) | 129 |
| ScrFI | CCNGG | 1 cut(s) | 216 |
| SetI | ASST | 8 cut(s) | 73, 134, 153, 246, 300, 381, 472, 548 |
| SinI | GGWCC | 1 cut(s) | 129 |
| SmlI | CTYRAG | 1 cut(s) | 358 |
| SmoI | CTYRAG | 1 cut(s) | 358 |
| Sse9I | AATT | 5 cut(s) | 160, 165, 476, 482, 495 |
| StyD4I | CCNGG | 1 cut(s) | 214 |
| TaaI | ACNGT | 1 cut(s) | 268 |
| TaiI | ACGT | 1 cut(s) | 381 |
| TaqI | TCGA | 2 cut(s) | 119, 369 |
| TasI | AATT | 5 cut(s) | 160, 165, 476, 482, 495 |
| TfiI | GAWTC | 2 cut(s) | 308, 406 |
| Tru1I | TTAA | 1 cut(s) | 233 |
| Tru9I | TTAA | 1 cut(s) | 233 |
| TscAI | CASTG | 2 cut(s) | 273, 525 |
| TseFI | GTSAC | 1 cut(s) | 457 |
| Tsp45I | GTSAC | 1 cut(s) | 457 |
| TspDTI | ATGAA | 2 cut(s) | 300, 336 |
| TspGWI | ACGGA | 1 cut(s) | 355 |
| TspRI | CASTG | 2 cut(s) | 273, 525 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 193 |
| VpaK11BI | GGWCC | 1 cut(s) | 129 |
| XapI | RAATTY | 3 cut(s) | 160, 476, 495 |
| XceI | RCATGY | 1 cut(s) | 226 |