The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom04g05930 pycom04g09980 pycom04g13690 pycom05g15520 pycom07g05540 pycom09g03940 pycom13g03240 pycom16g04970 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 96, 162 |
| AcsI | RAATTY | 2 cut(s) | 215, 233 |
| AcuI | CTGAAG | 1 cut(s) | 189 |
| AfaI | GTAC | 1 cut(s) | 179 |
| AgsI | TTSAA | 1 cut(s) | 221 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 207 |
| Alw26I | GTCTC | 1 cut(s) | 259 |
| AlwI | GGATC | 2 cut(s) | 96, 162 |
| AoxI | GGCC | 1 cut(s) | 36 |
| ApoI | RAATTY | 2 cut(s) | 215, 233 |
| AspLEI | GCGC | 2 cut(s) | 247, 256 |
| AsuHPI | GGTGA | 1 cut(s) | 54 |
| BauI | CACGAG | 1 cut(s) | 194 |
| BccI | CCATC | 2 cut(s) | 67, 73 |
| BcoDI | GTCTC | 1 cut(s) | 259 |
| BfoI | RGCGCY | 1 cut(s) | 248 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 207 |
| BpuEI | CTTGAG | 1 cut(s) | 82 |
| Bse1I | ACTGG | 1 cut(s) | 167 |
| BseNI | ACTGG | 1 cut(s) | 167 |
| BshFI | GGCC | 1 cut(s) | 38 |
| BsmAI | GTCTC | 1 cut(s) | 259 |
| BsmBI | CGTCTC | 1 cut(s) | 259 |
| BsnI | GGCC | 1 cut(s) | 38 |
| Bsp143I | GATC | 3 cut(s) | 101, 120, 154 |
| BspANI | GGCC | 1 cut(s) | 38 |
| BspPI | GGATC | 2 cut(s) | 96, 162 |
| BsrI | ACTGG | 1 cut(s) | 167 |
| BssMI | GATC | 3 cut(s) | 101, 120, 154 |
| BssSI | CACGAG | 1 cut(s) | 194 |
| Bst2BI | CACGAG | 1 cut(s) | 194 |
| Bst4CI | ACNGT | 1 cut(s) | 7 |
| BstH2I | RGCGCY | 1 cut(s) | 248 |
| BstHHI | GCGC | 2 cut(s) | 247, 256 |
| BstKTI | GATC | 3 cut(s) | 104, 123, 157 |
| BstMAI | GTCTC | 1 cut(s) | 259 |
| BstMBI | GATC | 3 cut(s) | 101, 120, 154 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 251, 253 |
| BstX2I | RGATCY | 2 cut(s) | 101, 154 |
| BstYI | RGATCY | 2 cut(s) | 101, 154 |
| BsuRI | GGCC | 1 cut(s) | 38 |
| BtsI | GCAGTG | 1 cut(s) | 263 |
| BtsIMutI | CAGTG | 3 cut(s) | 12, 244, 263 |
| CfoI | GCGC | 2 cut(s) | 247, 256 |
| Csp6I | GTAC | 1 cut(s) | 178 |
| CviAII | CATG | 1 cut(s) | 87 |
| CviJI | RGCY | 1 cut(s) | 38 |
| CviKI_1 | RGCY | 1 cut(s) | 38 |
| CviQI | GTAC | 1 cut(s) | 178 |
| DpnI | GATC | 3 cut(s) | 103, 122, 156 |
| DpnII | GATC | 3 cut(s) | 101, 120, 154 |
| DriI | GACNNNNNGTC | 1 cut(s) | 207 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 207 |
| Eco147I | AGGCCT | 1 cut(s) | 38 |
| Eco57I | CTGAAG | 1 cut(s) | 189 |
| EcoRI | GAATTC | 1 cut(s) | 233 |
| Esp3I | CGTCTC | 1 cut(s) | 259 |
| FaeI | CATG | 1 cut(s) | 90 |
| FaiI | YATR | 2 cut(s) | 88, 281 |
| FatI | CATG | 1 cut(s) | 86 |
| GlaI | GCGC | 2 cut(s) | 246, 255 |
| HaeII | RGCGCY | 1 cut(s) | 248 |
| HaeIII | GGCC | 1 cut(s) | 38 |
| HhaI | GCGC | 2 cut(s) | 247, 256 |
| Hin1II | CATG | 1 cut(s) | 90 |
| Hin6I | GCGC | 2 cut(s) | 245, 254 |
| HinP1I | GCGC | 2 cut(s) | 245, 254 |
| HincII | GTYRAC | 1 cut(s) | 15 |
| HindII | GTYRAC | 1 cut(s) | 15 |
| HinfI | GANTC | 2 cut(s) | 47, 145 |
| HphI | GGTGA | 1 cut(s) | 54 |
| Hpy166II | GTNNAC | 1 cut(s) | 15 |
| Hpy188I | TCNGA | 2 cut(s) | 139, 232 |
| Hpy188III | TCNNGA | 4 cut(s) | 99, 152, 160, 196 |
| Hpy8I | GTNNAC | 1 cut(s) | 15 |
| Hpy99I | CGWCG | 1 cut(s) | 110 |
| HpyCH4III | ACNGT | 1 cut(s) | 7 |
| HpyCH4IV | ACGT | 1 cut(s) | 117 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 251, 253 |
| HpySE526I | ACGT | 1 cut(s) | 117 |
| Hsp92II | CATG | 1 cut(s) | 90 |
| HspAI | GCGC | 2 cut(s) | 245, 254 |
| Kzo9I | GATC | 3 cut(s) | 101, 120, 154 |
| LpnPI | CCDG | 7 cut(s) | 52, 109, 137, 145, 180, 192, 236 |
| MaeII | ACGT | 1 cut(s) | 117 |
| MaeIII | GTNAC | 1 cut(s) | 196 |
| MalI | GATC | 3 cut(s) | 103, 122, 156 |
| MboI | GATC | 3 cut(s) | 101, 120, 154 |
| MboII | GAAGA | 1 cut(s) | 140 |
| MflI | RGATCY | 2 cut(s) | 101, 154 |
| MluCI | AATT | 3 cut(s) | 215, 221, 233 |
| MnlI | CCTC | 3 cut(s) | 13, 24, 28 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 251, 253 |
| NdeII | GATC | 3 cut(s) | 101, 120, 154 |
| NlaIII | CATG | 1 cut(s) | 90 |
| NmuCI | GTSAC | 1 cut(s) | 196 |
| PceI | AGGCCT | 1 cut(s) | 38 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 114 |
| PfeI | GAWTC | 2 cut(s) | 47, 145 |
| PsuI | RGATCY | 2 cut(s) | 101, 154 |
| RsaI | GTAC | 1 cut(s) | 179 |
| RsaNI | GTAC | 1 cut(s) | 178 |
| Sau3AI | GATC | 3 cut(s) | 101, 120, 154 |
| SetI | ASST | 3 cut(s) | 120, 211, 286 |
| SmlI | CTYRAG | 1 cut(s) | 97 |
| SmoI | CTYRAG | 1 cut(s) | 97 |
| Sse9I | AATT | 3 cut(s) | 215, 221, 233 |
| SseBI | AGGCCT | 1 cut(s) | 38 |
| StuI | AGGCCT | 1 cut(s) | 38 |
| TaaI | ACNGT | 1 cut(s) | 7 |
| TaiI | ACGT | 1 cut(s) | 120 |
| TaqI | TCGA | 1 cut(s) | 108 |
| TasI | AATT | 3 cut(s) | 215, 221, 233 |
| TfiI | GAWTC | 2 cut(s) | 47, 145 |
| TscAI | CASTG | 3 cut(s) | 12, 244, 263 |
| TseFI | GTSAC | 1 cut(s) | 196 |
| Tsp45I | GTSAC | 1 cut(s) | 196 |
| TspDTI | ATGAA | 2 cut(s) | 39, 75 |
| TspGWI | ACGGA | 2 cut(s) | 32, 94 |
| TspRI | CASTG | 3 cut(s) | 12, 244, 263 |
| XapI | RAATTY | 2 cut(s) | 215, 233 |