The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom04g05930 pycom04g09980 pycom04g13690 pycom05g15520 pycom07g05540 pycom09g03940 pycom13g03240 pycom16g04970 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 49 |
| AclWI | GGATC | 1 cut(s) | 213 |
| AcsI | RAATTY | 3 cut(s) | 16, 332, 351 |
| AcuI | CTGAAG | 1 cut(s) | 306 |
| AfaI | GTAC | 1 cut(s) | 296 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 49, 354 |
| AflIII | ACRYGT | 1 cut(s) | 78 |
| AgsI | TTSAA | 3 cut(s) | 21, 26, 338 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 324 |
| AjnI | CCWGG | 1 cut(s) | 70 |
| Alw26I | GTCTC | 2 cut(s) | 104, 377 |
| AlwI | GGATC | 1 cut(s) | 213 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 49 |
| ApoI | RAATTY | 3 cut(s) | 16, 332, 351 |
| AspLEI | GCGC | 2 cut(s) | 365, 374 |
| AsuHPI | GGTGA | 2 cut(s) | 171, 337 |
| BauI | CACGAG | 1 cut(s) | 311 |
| BccI | CCATC | 2 cut(s) | 184, 190 |
| BciT130I | CCWGG | 1 cut(s) | 72 |
| BcoDI | GTCTC | 2 cut(s) | 104, 377 |
| BfoI | RGCGCY | 1 cut(s) | 366 |
| Bme1390I | CCNGG | 1 cut(s) | 72 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 324 |
| BmrFI | CCNGG | 1 cut(s) | 72 |
| BpuEI | CTTGAG | 1 cut(s) | 199 |
| BsaJI | CCNNGG | 2 cut(s) | 70, 71 |
| BsaWI | WCCGGW | 1 cut(s) | 347 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 49, 354 |
| Bse1I | ACTGG | 1 cut(s) | 284 |
| BseBI | CCWGG | 1 cut(s) | 72 |
| BseDI | CCNNGG | 2 cut(s) | 70, 71 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 49, 354 |
| BseNI | ACTGG | 1 cut(s) | 284 |
| BsiSI | CCGG | 1 cut(s) | 348 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 49, 354 |
| BsmAI | GTCTC | 2 cut(s) | 104, 377 |
| BsmBI | CGTCTC | 1 cut(s) | 377 |
| Bsp143I | GATC | 2 cut(s) | 218, 237 |
| BspPI | GGATC | 1 cut(s) | 213 |
| BsrI | ACTGG | 1 cut(s) | 284 |
| BssECI | CCNNGG | 2 cut(s) | 70, 71 |
| BssMI | GATC | 2 cut(s) | 218, 237 |
| BssSI | CACGAG | 1 cut(s) | 311 |
| Bst2BI | CACGAG | 1 cut(s) | 311 |
| Bst2UI | CCWGG | 1 cut(s) | 72 |
| Bst4CI | ACNGT | 1 cut(s) | 124 |
| BstC8I | GCNNGC | 1 cut(s) | 51 |
| BstH2I | RGCGCY | 1 cut(s) | 366 |
| BstHHI | GCGC | 2 cut(s) | 365, 374 |
| BstKTI | GATC | 2 cut(s) | 221, 240 |
| BstMAI | GTCTC | 2 cut(s) | 104, 377 |
| BstMBI | GATC | 2 cut(s) | 218, 237 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 369, 371 |
| BstNI | CCWGG | 1 cut(s) | 72 |
| BstNSI | RCATGY | 1 cut(s) | 82 |
| BstSCI | CCNGG | 1 cut(s) | 70 |
| BstX2I | RGATCY | 1 cut(s) | 218 |
| BstYI | RGATCY | 1 cut(s) | 218 |
| BtsI | GCAGTG | 1 cut(s) | 381 |
| BtsIMutI | CAGTG | 2 cut(s) | 129, 381 |
| Cac8I | GCNNGC | 1 cut(s) | 51 |
| CaiI | CAGNNNCTG | 1 cut(s) | 49 |
| CfoI | GCGC | 2 cut(s) | 365, 374 |
| Csp6I | GTAC | 1 cut(s) | 295 |
| CviAII | CATG | 3 cut(s) | 65, 79, 204 |
| CviJI | RGCY | 1 cut(s) | 49 |
| CviKI_1 | RGCY | 1 cut(s) | 49 |
| CviQI | GTAC | 1 cut(s) | 295 |
| DpnI | GATC | 2 cut(s) | 220, 239 |
| DpnII | GATC | 2 cut(s) | 218, 237 |
| DriI | GACNNNNNGTC | 1 cut(s) | 324 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 324 |
| Eco57I | CTGAAG | 1 cut(s) | 306 |
| EcoRI | GAATTC | 1 cut(s) | 351 |
| EcoRII | CCWGG | 1 cut(s) | 70 |
| Esp3I | CGTCTC | 1 cut(s) | 377 |
| FaeI | CATG | 3 cut(s) | 68, 82, 207 |
| FaiI | YATR | 7 cut(s) | 66, 80, 115, 117, 119, 205, 399 |
| FatI | CATG | 3 cut(s) | 64, 78, 203 |
| GlaI | GCGC | 2 cut(s) | 364, 373 |
| HaeII | RGCGCY | 1 cut(s) | 366 |
| HapII | CCGG | 1 cut(s) | 348 |
| HhaI | GCGC | 2 cut(s) | 365, 374 |
| Hin1II | CATG | 3 cut(s) | 68, 82, 207 |
| Hin6I | GCGC | 2 cut(s) | 363, 372 |
| HinP1I | GCGC | 2 cut(s) | 363, 372 |
| HincII | GTYRAC | 1 cut(s) | 132 |
| HindII | GTYRAC | 1 cut(s) | 132 |
| HinfI | GANTC | 2 cut(s) | 164, 262 |
| HpaII | CCGG | 1 cut(s) | 348 |
| HphI | GGTGA | 2 cut(s) | 171, 337 |
| Hpy166II | GTNNAC | 1 cut(s) | 132 |
| Hpy188I | TCNGA | 1 cut(s) | 256 |
| Hpy188III | TCNNGA | 4 cut(s) | 216, 269, 277, 313 |
| Hpy8I | GTNNAC | 1 cut(s) | 132 |
| Hpy99I | CGWCG | 1 cut(s) | 227 |
| HpyAV | CCTTC | 1 cut(s) | 130 |
| HpyCH4III | ACNGT | 1 cut(s) | 124 |
| HpyCH4IV | ACGT | 1 cut(s) | 234 |
| HpyCH4V | TGCA | 1 cut(s) | 64 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 369, 371 |
| HpySE526I | ACGT | 1 cut(s) | 234 |
| Hsp92II | CATG | 3 cut(s) | 68, 82, 207 |
| HspAI | GCGC | 2 cut(s) | 363, 372 |
| Kzo9I | GATC | 2 cut(s) | 218, 237 |
| MaeII | ACGT | 1 cut(s) | 234 |
| MaeIII | GTNAC | 1 cut(s) | 313 |
| MalI | GATC | 2 cut(s) | 220, 239 |
| MboI | GATC | 2 cut(s) | 218, 237 |
| MboII | GAAGA | 1 cut(s) | 257 |
| MflI | RGATCY | 1 cut(s) | 218 |
| MluCI | AATT | 5 cut(s) | 16, 21, 332, 338, 351 |
| MnlI | CCTC | 4 cut(s) | 39, 110, 141, 145 |
| MseI | TTAA | 1 cut(s) | 89 |
| MspI | CCGG | 1 cut(s) | 348 |
| MspR9I | CCNGG | 1 cut(s) | 72 |
| MvaI | CCWGG | 1 cut(s) | 72 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 369, 371 |
| NdeII | GATC | 2 cut(s) | 218, 237 |
| NlaIII | CATG | 3 cut(s) | 68, 82, 207 |
| NmuCI | GTSAC | 1 cut(s) | 313 |
| NspI | RCATGY | 1 cut(s) | 82 |
| PasI | CCCWGGG | 1 cut(s) | 71 |
| PciI | ACATGT | 1 cut(s) | 78 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 231 |
| PfeI | GAWTC | 2 cut(s) | 164, 262 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 49 |
| PscI | ACATGT | 1 cut(s) | 78 |
| Psp6I | CCWGG | 1 cut(s) | 70 |
| PspGI | CCWGG | 1 cut(s) | 70 |
| PstNI | CAGNNNCTG | 1 cut(s) | 49 |
| PsuI | RGATCY | 1 cut(s) | 218 |
| RsaI | GTAC | 1 cut(s) | 296 |
| RsaNI | GTAC | 1 cut(s) | 295 |
| SaqAI | TTAA | 1 cut(s) | 89 |
| Sau3AI | GATC | 2 cut(s) | 218, 237 |
| ScrFI | CCNGG | 1 cut(s) | 72 |
| SetI | ASST | 6 cut(s) | 9, 102, 156, 237, 328, 404 |
| SmlI | CTYRAG | 1 cut(s) | 214 |
| SmoI | CTYRAG | 1 cut(s) | 214 |
| Sse9I | AATT | 5 cut(s) | 16, 21, 332, 338, 351 |
| StyD4I | CCNGG | 1 cut(s) | 70 |
| TaaI | ACNGT | 1 cut(s) | 124 |
| TaiI | ACGT | 1 cut(s) | 237 |
| TaqI | TCGA | 1 cut(s) | 225 |
| TasI | AATT | 5 cut(s) | 16, 21, 332, 338, 351 |
| TfiI | GAWTC | 2 cut(s) | 164, 262 |
| Tru1I | TTAA | 1 cut(s) | 89 |
| Tru9I | TTAA | 1 cut(s) | 89 |
| TscAI | CASTG | 2 cut(s) | 129, 381 |
| TseFI | GTSAC | 1 cut(s) | 313 |
| Tsp45I | GTSAC | 1 cut(s) | 313 |
| TspDTI | ATGAA | 2 cut(s) | 156, 192 |
| TspGWI | ACGGA | 1 cut(s) | 211 |
| TspRI | CASTG | 2 cut(s) | 129, 381 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 49 |
| XapI | RAATTY | 3 cut(s) | 16, 332, 351 |
| XceI | RCATGY | 1 cut(s) | 82 |