pycom07g14460

Mitogen-activated protein kinase kinase kinase 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0019322
Physical Location & Seq
Forward (+)
16405860 .. 16406534
675 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g14460.1

Sequence Viewer

Length: 675 bp
ATGGAGCAATTCCGAAGGATTGGGGAGGATGTAGGCAGCTTAAAGGCTTTAATGGTGTTCCAAGACAACATCCAAATCAATCAGCGGCAATGCTTTTTGATGCTCGATATCTTCAGCTCAGCATATGAATCAATAGCGGAGGAGATGAGACATAATCTTCGGTTTGATGAGAAGCACATGAAATGGAGGGTTCTCGAGCAGCCTCTGAGGGAGCTCCACAGGATATTCAAAGATGCAGAAGCCTACATTCGACAATGCTTGGATACCAAGGATTGGTGGGCTAAGGCCATCACACTCTATCAGAATTCCGATTGCGTAGAGTTTCACATCCACAACCTACTTGCCTGCATGCCGATCGTCATCGAAGCAATCGACATTGCTGGAGAAATATCGGGGTTGGATCAGGATGAAATACAGAGGAAGAAAACCATGTTCGCAGACAAGTACAAACAAGACTACAGAGATTGGAAACTTTTCAAGTGGAGATTTGGGAAGCAGTATTTGATTACTCAAGACTTTTGCAATAGGTTTGAATCAGCAGGAAATGAAGATAGATGGACCCTTCTGCATAAAATCAGCGGAAAGAAACTTTCGGGTTCAACAAAGTATGGGAAACGACTCATAGATCTTGGAGGCCATTCAAAAGCGCAGACGGATCAGAGTCGCAAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

225

Amino Acids

26.64

Weight (kDa)

8.5

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1221 PF06760 21 - 213 7e-95 Protein of unknown function (DUF1221)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019322)

Species Orthologous Gene IDs
pyrus_communis pycom07g14460
rosa_multiflora Rmu_sc0000388.1_g000046 Rmu_sc0004431.1_g000012 Rmu_ssc0000410.1_g000042
rosa_samantha Rh4CG094400 Rh5BG488600 Rh5DG305600 Rh7CG229500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 273
AciI CCGC 3 cut(s) 85, 137, 579
AclWI GGATC 2 cut(s) 408, 663
AcsI RAATTY 2 cut(s) 304, 669
AcuI CTGAAG 1 cut(s) 97
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 1 cut(s) 273
AgsI TTSAA 5 cut(s) 229, 478, 533, 600, 642
AluBI AGCT 3 cut(s) 39, 117, 214
AluI AGCT 3 cut(s) 39, 117, 214
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 142
AlwI GGATC 2 cut(s) 408, 663
AlwNI CAGNNNCTG 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 2 cut(s) 285, 634
ApeKI GCWGC 2 cut(s) 36, 199
ApoI RAATTY 2 cut(s) 304, 669
ArsI GACNNNNNNTTYG 2 cut(s) 141, 173
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 1 cut(s) 558
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 558
BanII GRGCYC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 2 cut(s) 48, 211
BccI CCATC 2 cut(s) 296, 549
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciVI GTATCC 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 142
BfmI CTRYAG 1 cut(s) 457
BfuI GTATCC 1 cut(s) 256
BglII AGATCT 1 cut(s) 625
BisI GCNGC 3 cut(s) 37, 86, 200
BlpI GCTNAGC 1 cut(s) 118
BlsI GCNGC 3 cut(s) 38, 87, 201
Bme18I GGWCC 1 cut(s) 558
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 1 cut(s) 560
BmsI GCATC 2 cut(s) 90, 223
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 118
BpuEI CTTGAG 1 cut(s) 495
BsaBI GATNNNNATC 1 cut(s) 359
BsaJI CCNNGG 1 cut(s) 267
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse3DI GCAATG 2 cut(s) 95, 375
Bse8I GATNNNNATC 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 4 cut(s) 34, 69, 327, 412
BseJI GATNNNNATC 1 cut(s) 359
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMI GCAATG 2 cut(s) 95, 375
BseMII CTCAG 2 cut(s) 132, 197
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 2 cut(s) 48, 211
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 2 cut(s) 287, 636
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 273
BsmAI GTCTC 1 cut(s) 142
BsnI GGCC 2 cut(s) 287, 636
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 4 cut(s) 354, 400, 625, 655
Bsp1720I GCTNAGC 1 cut(s) 118
BspACI CCGC 3 cut(s) 85, 137, 579
BspANI GGCC 2 cut(s) 287, 636
BspCNI CTCAG 2 cut(s) 131, 198
BspLI GGNNCC 1 cut(s) 560
BspPI GGATC 2 cut(s) 408, 663
BsrDI GCAATG 2 cut(s) 95, 375
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 4 cut(s) 354, 400, 625, 655
BssT1I CCWWGG 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 346, 350
BstDEI CTNAG 3 cut(s) 118, 206, 282
BstF5I GGATG 4 cut(s) 34, 69, 327, 412
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 4 cut(s) 357, 403, 628, 658
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 4 cut(s) 354, 400, 625, 655
BstMCI CGRYCG 1 cut(s) 357
BstNSI RCATGY 1 cut(s) 352
BstSFI CTRYAG 1 cut(s) 457
BstV1I GCAGC 2 cut(s) 48, 211
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BsuI GTATCC 1 cut(s) 256
BsuRI GGCC 2 cut(s) 287, 636
BtsCI GGATG 4 cut(s) 34, 69, 327, 412
Cac8I GCNNGC 2 cut(s) 346, 350
CaiI CAGNNNCTG 1 cut(s) 205
CfoI GCGC 1 cut(s) 649
Cfr13I GGNCC 1 cut(s) 558
Csp6I GTAC 1 cut(s) 445
CviAII CATG 3 cut(s) 178, 349, 430
CviJI RGCY 9 cut(s) 39, 47, 117, 202, 214, 242, 281, 287, 636
CviKI_1 RGCY 9 cut(s) 39, 47, 117, 202, 214, 242, 281, 287, 636
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 3 cut(s) 118, 206, 282
DpnI GATC 4 cut(s) 356, 402, 627, 657
DpnII GATC 4 cut(s) 354, 400, 625, 655
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 216
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 558
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 194
EcoICRI GAGCTC 1 cut(s) 214
EcoRI GAATTC 1 cut(s) 304
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 3 cut(s) 181, 352, 433
FaiI YATR 9 cut(s) 124, 126, 153, 179, 350, 431, 570, 609, 623
FatI CATG 3 cut(s) 177, 348, 429
FauNDI CATATG 1 cut(s) 124
Fnu4HI GCNGC 3 cut(s) 37, 86, 200
FokI GGATG 4 cut(s) 41, 56, 314, 419
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 3 cut(s) 37, 86, 200
GlaI GCGC 1 cut(s) 648
GluI GCNGC 3 cut(s) 37, 86, 200
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 2 cut(s) 287, 636
HhaI GCGC 1 cut(s) 649
Hin1II CATG 3 cut(s) 181, 352, 433
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HinfI GANTC 4 cut(s) 128, 533, 618, 661
Hpy188I TCNGA 5 cut(s) 14, 207, 303, 310, 660
Hpy188III TCNNGA 3 cut(s) 194, 404, 512
HpyAV CCTTC 2 cut(s) 9, 572
HpyCH4V TGCA 4 cut(s) 236, 348, 522, 568
HpyF3I CTNAG 3 cut(s) 118, 206, 282
Hsp92II CATG 3 cut(s) 181, 352, 433
HspAI GCGC 1 cut(s) 647
Kzo9I GATC 4 cut(s) 354, 400, 625, 655
LmnI GCTCC 3 cut(s) 4, 211, 219
LpnPI CCDG 5 cut(s) 205, 358, 366, 389, 525
Lsp1109I GCAGC 2 cut(s) 48, 211
LweI GCATC 2 cut(s) 90, 223
MalI GATC 4 cut(s) 356, 402, 627, 657
MboI GATC 4 cut(s) 354, 400, 625, 655
MboII GAAGA 4 cut(s) 103, 149, 433, 560
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 3 cut(s) 8, 304, 669
MlyI GAGTC 2 cut(s) 612, 670
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 7 cut(s) 19, 133, 180, 201, 213, 411, 626
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 2 cut(s) 41, 50
MspA1I CMGCKG 2 cut(s) 85, 579
NdeI CATATG 1 cut(s) 124
NdeII GATC 4 cut(s) 354, 400, 625, 655
NlaIII CATG 3 cut(s) 181, 352, 433
NlaIV GGNNCC 1 cut(s) 560
NspI RCATGY 1 cut(s) 352
PaeI GCATGC 1 cut(s) 352
PaeR7I CTCGAG 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 2 cut(s) 128, 533
PflMI CCANNNNNTGG 1 cut(s) 273
PkrI GCNGC 3 cut(s) 38, 87, 201
Ple19I CGATCG 1 cut(s) 357
PleI GAGTC 2 cut(s) 612, 669
PpsI GAGTC 2 cut(s) 612, 669
Psp124BI GAGCTC 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 560
PspPI GGNCC 1 cut(s) 558
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 1 cut(s) 625
PvuI CGATCG 1 cut(s) 357
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 2 cut(s) 41, 50
SatI GCNGC 3 cut(s) 37, 86, 200
Sau3AI GATC 4 cut(s) 354, 400, 625, 655
Sau96I GGNCC 1 cut(s) 558
SchI GAGTC 2 cut(s) 612, 670
SduI GDGCHC 1 cut(s) 216
SetI ASST 5 cut(s) 41, 119, 216, 339, 530
SfaNI GCATC 2 cut(s) 90, 223
SfcI CTRYAG 1 cut(s) 457
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 1 cut(s) 558
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 2 cut(s) 194, 510
SmoI CTYRAG 2 cut(s) 194, 510
SphI GCATGC 1 cut(s) 352
Sse9I AATT 3 cut(s) 8, 304, 669
SsiI CCGC 3 cut(s) 85, 137, 579
SstI GAGCTC 1 cut(s) 216
StyI CCWWGG 1 cut(s) 267
TaqI TCGA 5 cut(s) 105, 195, 250, 363, 372
TasI AATT 3 cut(s) 8, 304, 669
TatI WGTACW 1 cut(s) 444
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 2 cut(s) 128, 533
Tru1I TTAA 2 cut(s) 41, 50
Tru9I TTAA 2 cut(s) 41, 50
TseI GCWGC 2 cut(s) 36, 199
TspDTI ATGAA 4 cut(s) 141, 194, 423, 561
TspGWI ACGGA 1 cut(s) 668
Van91I CCANNNNNTGG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 558
XapI RAATTY 2 cut(s) 304, 669
XceI RCATGY 1 cut(s) 352
XhoI CTCGAG 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.