Rmu_sc0000388.1_g000046

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000388.1
OG0019322
Physical Location & Seq
Forward (+)
242317 .. 245484
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000388.1_g000046.1.cds

Sequence Viewer

Length: 885 bp
atggaggagcttcttatccggtggctacaccggtcccgattcttcactgagagtctccaactccgcccaccgagcccaatcgacttgatctgtccggctcttcgagatcgtgataaaccgccccccgttctcgagactcaatccctggtggctagtatggaggacaacgcgtcgagcccaacacggcattatttaatctcgtcctcaaggctatgcacggtgggtaagagatttaagtttgaagagccacgaatagaacagattcaagatcttgaggtttgtatttgtacagatcttgaagttgaaatggcctctgctgcttcggcttcaatcactgggtgcttagccggaatggaacaattccggcgtattggagaggttttggggagtatgaaggcactaatggtcttacaagatggtatcaagatcaatcaagggcaatgctgtctattgcttgatatcttgactttggcatttgagacaattggggaggagatcaggctgaacctgaaattggaggagaaaaagaccaaatggaagggcctagatgaacctctgagggagctctacacagttttcagagaaagcgagatttacatcagaagctgcatggatgccaaagactggtggggcaaagcagtcatcctccatcagaacaaggactgtgtcgaattttacatacataatttgttctgctacttctcggctgtcattgaggcaatcgagaacgctggagagattgcggggctggaccaggatgagatgcagaagaagaggattgtgcttaagaggaagtatgataaggattggaatgacccgaaacttttccagtggagatttgtttgtatcagcttttgctatttttcaatgttagctaggatgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

294

Amino Acids

34.14

Weight (kDa)

5.61

Isoelectric Point (pI)

61.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019322)

Species Orthologous Gene IDs
pyrus_communis pycom07g14460
rosa_multiflora Rmu_sc0000388.1_g000046 Rmu_sc0004431.1_g000012 Rmu_ssc0000410.1_g000042
rosa_samantha Rh4CG094400 Rh5BG488600 Rh5DG305600 Rh7CG229500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 624
AccII CGCG 1 cut(s) 170
AciI CCGC 3 cut(s) 64, 119, 743
AcsI RAATTY 1 cut(s) 671
AdeI CACNNNGTG 1 cut(s) 339
AfaI GTAC 1 cut(s) 289
AfiI CCNNNNNNNGG 2 cut(s) 514, 624
AflII CTTAAG 1 cut(s) 785
AflIII ACRYGT 1 cut(s) 168
AgeI ACCGGT 1 cut(s) 30
AgsI TTSAA 6 cut(s) 242, 266, 299, 305, 330, 867
AhdI GACNNNNNGTC 1 cut(s) 169
AjnI CCWGG 2 cut(s) 144, 753
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 5 cut(s) 10, 565, 606, 852, 875
AluI AGCT 5 cut(s) 10, 565, 606, 852, 875
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 3 cut(s) 59, 128, 473
AlwNI CAGNNNCTG 1 cut(s) 606
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 2 cut(s) 309, 541
ApeKI GCWGC 2 cut(s) 317, 606
ApoI RAATTY 1 cut(s) 671
AsiGI ACCGGT 1 cut(s) 30
Asp700I GAANNNNTTC 2 cut(s) 261, 824
AspS9I GGNCC 3 cut(s) 33, 541, 751
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 2 cut(s) 33, 751
BanII GRGCYC 3 cut(s) 77, 179, 567
Bbv12I GWGCWC 1 cut(s) 567
BbvI GCAGC 2 cut(s) 304, 593
BccI CCATC 2 cut(s) 410, 657
BceAI ACGGC 1 cut(s) 200
BciT130I CCWGG 2 cut(s) 146, 755
BcoDI GTCTC 3 cut(s) 59, 128, 473
BfaI CTAG 3 cut(s) 153, 545, 876
BfrI CTTAAG 1 cut(s) 785
BglII AGATCT 2 cut(s) 268, 292
BisI GCNGC 2 cut(s) 318, 607
BlpI GCTNAGC 1 cut(s) 343
BlsI GCNGC 2 cut(s) 319, 608
Bme1390I CCNGG 2 cut(s) 146, 755
Bme18I GGWCC 2 cut(s) 33, 751
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 3 cut(s) 33, 541, 751
BmiI GGNNCC 1 cut(s) 35
BmrFI CCNGG 2 cut(s) 146, 755
BmrI ACTGGG 1 cut(s) 345
BmsI GCATC 2 cut(s) 604, 753
BmuI ACTGGG 1 cut(s) 345
BpmI CTGGAG 1 cut(s) 753
Bpu1102I GCTNAGC 1 cut(s) 343
BpuEI CTTGAG 2 cut(s) 190, 293
BsaBI GATNNNNATC 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 2 cut(s) 18, 30
Bsc4I CCNNNNNNNGG 2 cut(s) 514, 624
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 3 cut(s) 340, 629, 829
Bse3DI GCAATG 1 cut(s) 446
Bse8I GATNNNNATC 1 cut(s) 596
BseBI CCWGG 2 cut(s) 146, 755
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 4 cut(s) 619, 642, 763, 885
BseJI GATNNNNATC 1 cut(s) 596
BseLI CCNNNNNNNGG 2 cut(s) 514, 624
BseMI GCAATG 1 cut(s) 446
BseMII CTCAG 2 cut(s) 39, 548
BseNI ACTGG 3 cut(s) 340, 629, 829
BseRI GAGGAG 3 cut(s) 20, 506, 533
BseXI GCAGC 2 cut(s) 304, 593
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 2 cut(s) 311, 543
BshTI ACCGGT 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 5 cut(s) 19, 31, 95, 348, 364
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 2 cut(s) 514, 624
BsmAI GTCTC 3 cut(s) 59, 128, 473
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 2 cut(s) 311, 543
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 3 cut(s) 77, 179, 567
Bsp1407I TGTACA 1 cut(s) 287
Bsp143I GATC 6 cut(s) 87, 106, 268, 292, 426, 495
Bsp1720I GCTNAGC 1 cut(s) 343
BspACI CCGC 3 cut(s) 64, 119, 743
BspANI GGCC 2 cut(s) 311, 543
BspCNI CTCAG 2 cut(s) 40, 549
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 1 cut(s) 35
BspQI GCTCTTC 2 cut(s) 105, 237
BspTI CTTAAG 1 cut(s) 785
BsrDI GCAATG 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 30
BsrGI TGTACA 1 cut(s) 287
BsrI ACTGG 3 cut(s) 340, 629, 829
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 6 cut(s) 87, 106, 268, 292, 426, 495
Bst2UI CCWGG 2 cut(s) 146, 755
Bst4CI ACNGT 3 cut(s) 220, 574, 665
Bst6I CTCTTC 3 cut(s) 105, 237, 767
BstAFI CTTAAG 1 cut(s) 785
BstAUI TGTACA 1 cut(s) 287
BstDEI CTNAG 3 cut(s) 48, 343, 557
BstF5I GGATG 4 cut(s) 619, 642, 763, 885
BstFNI CGCG 1 cut(s) 170
BstKTI GATC 6 cut(s) 90, 109, 271, 295, 429, 498
BstMAI GTCTC 3 cut(s) 59, 128, 473
BstMBI GATC 6 cut(s) 87, 106, 268, 292, 426, 495
BstMWI GCNNNNNNNGC 3 cut(s) 72, 317, 323
BstNI CCWGG 2 cut(s) 146, 755
BstSCI CCNGG 2 cut(s) 144, 753
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 2 cut(s) 304, 593
BstX2I RGATCY 2 cut(s) 268, 292
BstYI RGATCY 2 cut(s) 268, 292
BsuRI GGCC 2 cut(s) 311, 543
BtsCI GGATG 4 cut(s) 619, 642, 763, 885
BtsIMutI CAGTG 3 cut(s) 45, 333, 836
CaiI CAGNNNCTG 1 cut(s) 606
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 3 cut(s) 33, 541, 751
CseI GACGC 1 cut(s) 159
Csp6I GTAC 1 cut(s) 288
CspAI ACCGGT 1 cut(s) 30
CviAII CATG 1 cut(s) 610
CviQI GTAC 1 cut(s) 288
DdeI CTNAG 3 cut(s) 48, 343, 557
DpnI GATC 6 cut(s) 89, 108, 270, 294, 428, 497
DpnII GATC 6 cut(s) 87, 106, 268, 292, 426, 495
DraIII CACNNNGTG 1 cut(s) 339
DriI GACNNNNNGTC 1 cut(s) 169
Eam1104I CTCTTC 3 cut(s) 105, 237, 767
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 3 cut(s) 105, 237, 767
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 565
Eco24I GRGCYC 3 cut(s) 77, 179, 567
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 2 cut(s) 33, 751
Eco53kI GAGCTC 1 cut(s) 565
Eco88I CYCGRG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 565
EcoO109I RGGNCCY 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 144, 753
EcoRV GATATC 1 cut(s) 460
EcoT38I GRGCYC 3 cut(s) 77, 179, 567
FaeI CATG 1 cut(s) 613
FaiI YATR 7 cut(s) 158, 214, 392, 611, 680, 684, 798
FaqI GGGAC 1 cut(s) 19
FatI CATG 1 cut(s) 609
FauI CCCGC 1 cut(s) 736
Fnu4HI GCNGC 2 cut(s) 318, 607
FokI GGATG 3 cut(s) 626, 629, 770
FriOI GRGCYC 3 cut(s) 77, 179, 567
Fsp4HI GCNGC 2 cut(s) 318, 607
FspBI CTAG 3 cut(s) 153, 545, 876
GluI GCNGC 2 cut(s) 318, 607
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 2 cut(s) 311, 543
HapII CCGG 5 cut(s) 19, 31, 95, 348, 364
HgaI GACGC 1 cut(s) 159
Hin1II CATG 1 cut(s) 613
HinfI GANTC 4 cut(s) 39, 52, 136, 262
HpaII CCGG 5 cut(s) 19, 31, 95, 348, 364
Hpy188I TCNGA 4 cut(s) 558, 581, 602, 654
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 2 cut(s) 388, 532
HpyCH4III ACNGT 3 cut(s) 220, 574, 665
HpyCH4V TGCA 3 cut(s) 216, 609, 766
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 317, 323
HpyF3I CTNAG 3 cut(s) 48, 343, 557
Hsp92II CATG 1 cut(s) 613
Kzo9I GATC 6 cut(s) 87, 106, 268, 292, 426, 495
LguI GCTCTTC 2 cut(s) 105, 237
LmnI GCTCC 2 cut(s) 7, 562
Lsp1109I GCAGC 2 cut(s) 304, 593
LweI GCATC 2 cut(s) 604, 753
MaeI CTAG 3 cut(s) 153, 545, 876
MalI GATC 6 cut(s) 89, 108, 270, 294, 428, 497
MboI GATC 6 cut(s) 87, 106, 268, 292, 426, 495
MboII GAAGA 5 cut(s) 34, 92, 254, 781, 784
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 2 cut(s) 268, 292
MhlI GDGCHC 3 cut(s) 77, 179, 567
MluCI AATT 5 cut(s) 359, 483, 512, 671, 685
MluI ACGCGT 1 cut(s) 168
MlyI GAGTC 2 cut(s) 61, 130
MmeI TCCRAC 1 cut(s) 82
MroXI GAANNNNTTC 2 cut(s) 261, 824
MseI TTAA 3 cut(s) 194, 234, 786
MspCI CTTAAG 1 cut(s) 785
MspI CCGG 5 cut(s) 19, 31, 95, 348, 364
MspR9I CCNGG 2 cut(s) 146, 755
MunI CAATTG 1 cut(s) 483
MvaI CCWGG 2 cut(s) 146, 755
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 3 cut(s) 72, 317, 323
NdeII GATC 6 cut(s) 87, 106, 268, 292, 426, 495
NlaIII CATG 1 cut(s) 613
NlaIV GGNNCC 1 cut(s) 35
NmeAIII GCCGAG 1 cut(s) 683
PaeR7I CTCGAG 1 cut(s) 131
PciSI GCTCTTC 2 cut(s) 105, 237
PdmI GAANNNNTTC 2 cut(s) 261, 824
PfeI GAWTC 2 cut(s) 39, 262
PflFI GACNNNGTC 1 cut(s) 665
PflMI CCANNNNNTGG 1 cut(s) 624
PinAI ACCGGT 1 cut(s) 30
PkrI GCNGC 2 cut(s) 319, 608
PleI GAGTC 2 cut(s) 60, 130
PpsI GAGTC 2 cut(s) 60, 130
Psp124BI GAGCTC 1 cut(s) 567
Psp6I CCWGG 2 cut(s) 144, 753
PspGI CCWGG 2 cut(s) 144, 753
PspN4I GGNNCC 1 cut(s) 35
PspPI GGNCC 3 cut(s) 33, 541, 751
PstNI CAGNNNCTG 1 cut(s) 606
PsuI RGATCY 2 cut(s) 268, 292
PsyI GACNNNGTC 1 cut(s) 665
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
SacI GAGCTC 1 cut(s) 567
SapI GCTCTTC 2 cut(s) 105, 237
SaqAI TTAA 3 cut(s) 194, 234, 786
SatI GCNGC 2 cut(s) 318, 607
Sau3AI GATC 6 cut(s) 87, 106, 268, 292, 426, 495
Sau96I GGNCC 3 cut(s) 33, 541, 751
SchI GAGTC 2 cut(s) 61, 130
ScrFI CCNGG 2 cut(s) 146, 755
SduI GDGCHC 3 cut(s) 77, 179, 567
SetI ASST 9 cut(s) 12, 279, 381, 510, 556, 567, 608, 854, 877
SfaNI GCATC 2 cut(s) 604, 753
Sfr274I CTCGAG 1 cut(s) 131
SinI GGWCC 2 cut(s) 33, 751
SlaI CTCGAG 1 cut(s) 131
SmlI CTYRAG 4 cut(s) 131, 205, 272, 785
SmoI CTYRAG 4 cut(s) 131, 205, 272, 785
Sse9I AATT 5 cut(s) 359, 483, 512, 671, 685
SsiI CCGC 3 cut(s) 64, 119, 743
SspMI CTAG 3 cut(s) 153, 545, 876
SstI GAGCTC 1 cut(s) 567
StyD4I CCNGG 2 cut(s) 144, 753
TaaI ACNGT 3 cut(s) 220, 574, 665
TaqI TCGA 6 cut(s) 81, 103, 132, 173, 669, 723
TasI AATT 5 cut(s) 359, 483, 512, 671, 685
TatI WGTACW 1 cut(s) 287
TfiI GAWTC 2 cut(s) 39, 262
Tru1I TTAA 3 cut(s) 194, 234, 786
Tru9I TTAA 3 cut(s) 194, 234, 786
TscAI CASTG 3 cut(s) 52, 340, 836
TseI GCWGC 2 cut(s) 317, 606
TspDTI ATGAA 2 cut(s) 407, 564
TspRI CASTG 3 cut(s) 52, 340, 836
Tth111I GACNNNGTC 1 cut(s) 665
Van91I CCANNNNNTGG 1 cut(s) 624
Vha464I CTTAAG 1 cut(s) 785
VpaK11BI GGWCC 2 cut(s) 33, 751
XapI RAATTY 1 cut(s) 671
XhoI CTCGAG 1 cut(s) 131
XmnI GAANNNNTTC 2 cut(s) 261, 824
XspI CTAG 3 cut(s) 153, 545, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.