Rh5BG488600

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0019322
Physical Location & Seq
Reverse (-)
77811396 .. 77817008
5613 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG488600.1

Sequence Viewer

Length: 879 bp
ATGGAACAATTCCGGCGTATTGGAGAGGTTTTGGGGAGTATGAAGGCACTGATGGTCTTACAAGATGGTATCAAGATCAATCAAGGGCAGTGCTGTCTATTGCTTGATATCTTGACTTTGGCATTTGAGACAATTGGGGAGGAGATCAGGCTGAACCTGAAATTGGAGGAGAAAAAGACCAAATGGAAGGGCCTAGATGAACCTCTGAGGGAGCTCTACACAGTTTTCAGAGAAAGCGAGATTTACATCAGAAGCTGCATGGATGCCAAAGACTGGTGGGGCAAAGCAGTCATCCTCCATCAGAACAAGGACTGTGTCGAATTTTACATACATAATTTGTTCTGCTACTTCTCGGCTGTCATTGAGGCAATCGAGAACGCTGGAGAGATTGCGGTGCTGGACCAGGATGAGATGCAGAAGAAGAGGATTGTGCTTAAGAGGAAGTATGATAAGGATTGGAATGACCCGAAACTTTTCCAATGGAGATTTGCTATTCCGGGTTTGGGTGCTGCAGCTGGACCAATGCAGGTAGAGGCGCTTCCATCTGCTCCGTCGTCTCAAATTCTTGGGATGTGTTGGGACTTTCCAGTGGAATTAGTTAGGTTAGGGAAGAGACCTATGTTTGTGGAGGTTATGGTGATTGTTGCTCTATTGGATGTGGAGGAAGACTTTGTATTCCAAATACTAAAGGTGTGGTGCATCTGTCTCCTGCAAAGAAGAAGAAAGGTTGGTCTAAAGGCGAAGAACAAAGCTAAAGTTAATGAGAATGGGACTAGACCAAAAAAAAAGAGGTTTCCTTACAAGCTTAAACTTAGTGGAGATGCAGAGGTGGTGGAGGCTGTGGTAGCGGTTATGGTGGAGGAAATGGCAATGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

292

Amino Acids

33.65

Weight (kDa)

8.31

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1221 PF06760 21 - 164 2.2e-71 Protein of unknown function (DUF1221)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019322)

Species Orthologous Gene IDs
pyrus_communis pycom07g14460
rosa_multiflora Rmu_sc0000388.1_g000046 Rmu_sc0004431.1_g000012 Rmu_ssc0000410.1_g000042
rosa_samantha Rh4CG094400 Rh5BG488600 Rh5DG305600 Rh7CG229500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 517
AccB7I CCANNNNNTGG 1 cut(s) 273
AciI CCGC 2 cut(s) 392, 848
AcsI RAATTY 2 cut(s) 320, 561
AfiI CCNNNNNNNGG 3 cut(s) 163, 273, 503
AflII CTTAAG 1 cut(s) 434
AjnI CCWGG 1 cut(s) 402
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 5 cut(s) 214, 255, 515, 752, 805
AluI AGCT 5 cut(s) 214, 255, 515, 752, 805
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 4 cut(s) 122, 561, 607, 710
AlwNI CAGNNNCTG 1 cut(s) 255
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 3 cut(s) 255, 509, 512
ApoI RAATTY 2 cut(s) 320, 561
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 1 cut(s) 538
AspS9I GGNCC 3 cut(s) 190, 400, 518
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 1 cut(s) 649
AvaII GGWCC 2 cut(s) 400, 518
BanII GRGCYC 1 cut(s) 216
BarI GAAGNNNNNNTAC 2 cut(s) 522, 554
BbsI GAAGAC 1 cut(s) 672
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 3 cut(s) 242, 496, 524
BccI CCATC 4 cut(s) 46, 59, 306, 550
BciT130I CCWGG 1 cut(s) 404
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 4 cut(s) 122, 561, 607, 710
BfaI CTAG 2 cut(s) 194, 774
BfmI CTRYAG 1 cut(s) 510
BfoI RGCGCY 1 cut(s) 539
BfrI CTTAAG 1 cut(s) 434
BfuAI ACCTGC 1 cut(s) 517
BisI GCNGC 3 cut(s) 256, 510, 513
BlsI GCNGC 3 cut(s) 257, 511, 514
Bme1390I CCNGG 2 cut(s) 404, 498
Bme18I GGWCC 2 cut(s) 400, 518
BmgT120I GGNCC 3 cut(s) 190, 400, 518
BmrFI CCNGG 2 cut(s) 404, 498
BmsI GCATC 4 cut(s) 253, 402, 708, 811
BpiI GAAGAC 1 cut(s) 672
BpmI CTGGAG 1 cut(s) 402
BpuMI CCSGG 1 cut(s) 498
BsaBI GATNNNNATC 1 cut(s) 245
BsaI GGTCTC 1 cut(s) 607
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 273, 503
Bse1I ACTGG 2 cut(s) 278, 587
Bse3DI GCAATG 1 cut(s) 876
Bse8I GATNNNNATC 1 cut(s) 245
BseBI CCWGG 1 cut(s) 404
BseGI GGATG 5 cut(s) 268, 291, 412, 576, 661
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 3 cut(s) 163, 273, 503
BseMI GCAATG 1 cut(s) 876
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 2 cut(s) 278, 587
BseRI GAGGAG 2 cut(s) 155, 182
BseXI GCAGC 3 cut(s) 242, 496, 524
BshFI GGCC 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 2 cut(s) 13, 497
BslFI GGGAC 2 cut(s) 593, 784
BslI CCNNNNNNNGG 3 cut(s) 163, 273, 503
BsmAI GTCTC 4 cut(s) 122, 561, 607, 710
BsmBI CGTCTC 1 cut(s) 561
BsmFI GGGAC 2 cut(s) 593, 784
BsnI GGCC 1 cut(s) 192
Bso31I GGTCTC 1 cut(s) 607
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 75, 144
BspACI CCGC 2 cut(s) 392, 848
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 514
BspMI ACCTGC 1 cut(s) 517
BspTI CTTAAG 1 cut(s) 434
BspTNI GGTCTC 1 cut(s) 607
BsrDI GCAATG 1 cut(s) 876
BsrI ACTGG 2 cut(s) 278, 587
BssMI GATC 2 cut(s) 75, 144
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 2 cut(s) 223, 314
Bst6I CTCTTC 2 cut(s) 416, 605
BstAFI CTTAAG 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 206, 812
BstF5I GGATG 5 cut(s) 268, 291, 412, 576, 661
BstH2I RGCGCY 1 cut(s) 539
BstHHI GCGC 1 cut(s) 538
BstKTI GATC 2 cut(s) 78, 147
BstMAI GTCTC 4 cut(s) 122, 561, 607, 710
BstMBI GATC 2 cut(s) 75, 144
BstMWI GCNNNNNNNGC 1 cut(s) 845
BstNI CCWGG 1 cut(s) 404
BstSCI CCNGG 2 cut(s) 402, 496
BstSFI CTRYAG 1 cut(s) 510
BstV1I GCAGC 3 cut(s) 242, 496, 524
BstV2I GAAGAC 1 cut(s) 672
BsuRI GGCC 1 cut(s) 192
BtsCI GGATG 5 cut(s) 268, 291, 412, 576, 661
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 3 cut(s) 47, 95, 594
BveI ACCTGC 1 cut(s) 517
CaiI CAGNNNCTG 1 cut(s) 255
CfoI GCGC 1 cut(s) 538
Cfr13I GGNCC 3 cut(s) 190, 400, 518
CviAII CATG 1 cut(s) 259
CviJI RGCY 9 cut(s) 151, 192, 214, 255, 356, 515, 752, 805, 839
CviKI_1 RGCY 9 cut(s) 151, 192, 214, 255, 356, 515, 752, 805, 839
DdeI CTNAG 2 cut(s) 206, 812
DpnI GATC 2 cut(s) 77, 146
DpnII GATC 2 cut(s) 75, 144
Eam1104I CTCTTC 2 cut(s) 416, 605
EarI CTCTTC 2 cut(s) 416, 605
Ecl136II GAGCTC 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 607
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 2 cut(s) 400, 518
Eco53kI GAGCTC 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 402
EcoRV GATATC 1 cut(s) 109
EcoT38I GRGCYC 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 561
FaeI CATG 1 cut(s) 262
FaiI YATR 8 cut(s) 41, 260, 329, 333, 447, 620, 635, 854
FaqI GGGAC 2 cut(s) 593, 784
FatI CATG 1 cut(s) 258
Fnu4HI GCNGC 3 cut(s) 256, 510, 513
FokI GGATG 5 cut(s) 275, 278, 419, 583, 668
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 3 cut(s) 256, 510, 513
FspBI CTAG 2 cut(s) 194, 774
GlaI GCGC 1 cut(s) 537
GluI GCNGC 3 cut(s) 256, 510, 513
GsuI CTGGAG 1 cut(s) 402
HaeII RGCGCY 1 cut(s) 539
HaeIII GGCC 1 cut(s) 192
HapII CCGG 2 cut(s) 13, 497
HhaI GCGC 1 cut(s) 538
Hin1II CATG 1 cut(s) 262
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HindIII AAGCTT 1 cut(s) 803
HpaII CCGG 2 cut(s) 13, 497
HphI GGTGA 1 cut(s) 649
Hpy188I TCNGA 4 cut(s) 207, 230, 251, 303
Hpy188III TCNNGA 3 cut(s) 73, 112, 373
Hpy99I CGWCG 1 cut(s) 556
HpyAV CCTTC 2 cut(s) 37, 181
HpyCH4III ACNGT 2 cut(s) 223, 314
HpyCH4V TGCA 7 cut(s) 258, 415, 512, 526, 699, 712, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 845
HpyF3I CTNAG 2 cut(s) 206, 812
Hsp92II CATG 1 cut(s) 262
HspAI GCGC 1 cut(s) 536
Kzo9I GATC 2 cut(s) 75, 144
LmnI GCTCC 2 cut(s) 211, 553
Lsp1109I GCAGC 3 cut(s) 242, 496, 524
LweI GCATC 4 cut(s) 253, 402, 708, 811
MaeI CTAG 2 cut(s) 194, 774
MalI GATC 2 cut(s) 77, 146
MboI GATC 2 cut(s) 75, 144
MboII GAAGA 7 cut(s) 430, 433, 622, 677, 729, 732, 754
MfeI CAATTG 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 7 cut(s) 8, 132, 161, 320, 334, 561, 593
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 4 cut(s) 435, 759, 807, 877
MspA1I CMGCKG 1 cut(s) 515
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 2 cut(s) 13, 497
MspR9I CCNGG 2 cut(s) 404, 498
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 404
MwoI GCNNNNNNNGC 1 cut(s) 845
NciI CCSGG 1 cut(s) 498
NdeII GATC 2 cut(s) 75, 144
NlaIII CATG 1 cut(s) 262
NmeAIII GCCGAG 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 473
PflFI GACNNNGTC 1 cut(s) 314
PflMI CCANNNNNTGG 1 cut(s) 273
PkrI GCNGC 3 cut(s) 257, 511, 514
Psp124BI GAGCTC 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 402
PspGI CCWGG 1 cut(s) 402
PspPI GGNCC 3 cut(s) 190, 400, 518
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 255
PsyI GACNNNGTC 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 515
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 4 cut(s) 435, 759, 807, 877
SatI GCNGC 3 cut(s) 256, 510, 513
Sau3AI GATC 2 cut(s) 75, 144
Sau96I GGNCC 3 cut(s) 190, 400, 518
ScrFI CCNGG 2 cut(s) 404, 498
SduI GDGCHC 1 cut(s) 216
SfaNI GCATC 4 cut(s) 253, 402, 708, 811
SfcI CTRYAG 1 cut(s) 510
SinI GGWCC 2 cut(s) 400, 518
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 7 cut(s) 8, 132, 161, 320, 334, 561, 593
SsiI CCGC 2 cut(s) 392, 848
SspMI CTAG 2 cut(s) 194, 774
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 402, 496
TaaI ACNGT 2 cut(s) 223, 314
TaqI TCGA 2 cut(s) 318, 372
TasI AATT 7 cut(s) 8, 132, 161, 320, 334, 561, 593
Tru1I TTAA 4 cut(s) 435, 759, 807, 877
Tru9I TTAA 4 cut(s) 435, 759, 807, 877
TscAI CASTG 3 cut(s) 54, 95, 594
TseI GCWGC 3 cut(s) 255, 509, 512
TspDTI ATGAA 2 cut(s) 56, 213
TspGWI ACGGA 1 cut(s) 540
TspRI CASTG 3 cut(s) 54, 95, 594
Tth111I GACNNNGTC 1 cut(s) 314
Van91I CCANNNNNTGG 1 cut(s) 273
Vha464I CTTAAG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 400, 518
XapI RAATTY 2 cut(s) 320, 561
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 2 cut(s) 194, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.