Rh5DG305600

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0019322
Physical Location & Seq
Reverse (-)
38590559 .. 38590975
417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG305600.1

Sequence Viewer

Length: 417 bp
ATGGAACAATTCCGGCGTATTGGAGAGGTTTTGGGGAGTATGAAGGCACTGATGGTCTTACAAGATGGTATCAAGATCAATCAAGGGCAGTGCTGTCTATTGCTTGATATCTTGACTTTGGCATTTGAGACAATTGGGGAGGAGATCAGGCTGAACCTGAAATTGGAGGAGAAAAAGACCAAATGGAAGGGCCTAGATGAACCTCTGAGGGAGCTCTACACAGTTTTCAAAGAAAGCGAGATTTACATCAGAAGCTGCATGGATGCCAAAGACTGGTGGGGCAAAGCAGTCATCCTCCATCAGAACAAGGACTGTGTCCAATTTTACATACATAATTTGTTCTGCTACTTCTCGGCTGTCATTGAGGCAATCGAGAACGCTGGAGAGATTGCGGGGCTGGACCAGGATGAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

138

Amino Acids

16.0

Weight (kDa)

4.88

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1221 PF06760 21 - 138 2.5e-58 Protein of unknown function (DUF1221)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019322)

Species Orthologous Gene IDs
pyrus_communis pycom07g14460
rosa_multiflora Rmu_sc0000388.1_g000046 Rmu_sc0004431.1_g000012 Rmu_ssc0000410.1_g000042
rosa_samantha Rh4CG094400 Rh5BG488600 Rh5DG305600 Rh7CG229500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 273
AciI CCGC 1 cut(s) 392
AfiI CCNNNNNNNGG 2 cut(s) 163, 273
AgsI TTSAA 1 cut(s) 229
AjnI CCWGG 1 cut(s) 402
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 2 cut(s) 214, 255
AluI AGCT 2 cut(s) 214, 255
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 122
AlwNI CAGNNNCTG 1 cut(s) 255
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 1 cut(s) 255
AspS9I GGNCC 2 cut(s) 190, 400
AvaII GGWCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 242
BccI CCATC 3 cut(s) 46, 59, 306
BciT130I CCWGG 1 cut(s) 404
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 1 cut(s) 194
BisI GCNGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 404
Bme18I GGWCC 1 cut(s) 400
BmgT120I GGNCC 2 cut(s) 190, 400
BmrFI CCNGG 1 cut(s) 404
BmsI GCATC 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 402
BsaBI GATNNNNATC 1 cut(s) 245
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 273
Bse1I ACTGG 1 cut(s) 278
Bse8I GATNNNNATC 1 cut(s) 245
BseBI CCWGG 1 cut(s) 404
BseGI GGATG 3 cut(s) 268, 291, 412
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 2 cut(s) 163, 273
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 1 cut(s) 278
BseRI GAGGAG 2 cut(s) 155, 182
BseXI GCAGC 1 cut(s) 242
BshFI GGCC 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 1 cut(s) 13
BslI CCNNNNNNNGG 2 cut(s) 163, 273
BsmAI GTCTC 1 cut(s) 122
BsnI GGCC 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 75, 144
BspACI CCGC 1 cut(s) 392
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 1 cut(s) 198
BsrI ACTGG 1 cut(s) 278
BssMI GATC 2 cut(s) 75, 144
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 2 cut(s) 223, 314
BstDEI CTNAG 1 cut(s) 206
BstF5I GGATG 3 cut(s) 268, 291, 412
BstKTI GATC 2 cut(s) 78, 147
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 2 cut(s) 75, 144
BstNI CCWGG 1 cut(s) 404
BstSCI CCNGG 1 cut(s) 402
BstV1I GCAGC 1 cut(s) 242
BsuRI GGCC 1 cut(s) 192
BtsCI GGATG 3 cut(s) 268, 291, 412
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 47, 95
CaiI CAGNNNCTG 1 cut(s) 255
Cfr13I GGNCC 2 cut(s) 190, 400
CviAII CATG 1 cut(s) 259
CviJI RGCY 6 cut(s) 151, 192, 214, 255, 356, 397
CviKI_1 RGCY 6 cut(s) 151, 192, 214, 255, 356, 397
DdeI CTNAG 1 cut(s) 206
DpnI GATC 2 cut(s) 77, 146
DpnII GATC 2 cut(s) 75, 144
Ecl136II GAGCTC 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 216
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 402
EcoRV GATATC 1 cut(s) 109
EcoT38I GRGCYC 1 cut(s) 216
FaeI CATG 1 cut(s) 262
FaiI YATR 4 cut(s) 41, 260, 329, 333
FatI CATG 1 cut(s) 258
FauI CCCGC 1 cut(s) 385
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 2 cut(s) 275, 278
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 1 cut(s) 256
FspBI CTAG 1 cut(s) 194
GluI GCNGC 1 cut(s) 256
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 1 cut(s) 192
HapII CCGG 1 cut(s) 13
Hin1II CATG 1 cut(s) 262
HpaII CCGG 1 cut(s) 13
Hpy188I TCNGA 3 cut(s) 207, 251, 303
Hpy188III TCNNGA 3 cut(s) 73, 112, 373
HpyAV CCTTC 2 cut(s) 37, 181
HpyCH4III ACNGT 2 cut(s) 223, 314
HpyCH4V TGCA 1 cut(s) 258
HpyF3I CTNAG 1 cut(s) 206
Hsp92II CATG 1 cut(s) 262
Kzo9I GATC 2 cut(s) 75, 144
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 7 cut(s) 26, 133, 170, 259, 366, 383, 389
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 1 cut(s) 253
MaeI CTAG 1 cut(s) 194
MalI GATC 2 cut(s) 77, 146
MboI GATC 2 cut(s) 75, 144
MfeI CAATTG 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 5 cut(s) 8, 132, 161, 320, 334
MnlI CCTC 7 cut(s) 19, 133, 160, 201, 213, 305, 358
MspI CCGG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 404
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 404
NdeII GATC 2 cut(s) 75, 144
NlaIII CATG 1 cut(s) 262
NmeAIII GCCGAG 1 cut(s) 332
PflFI GACNNNGTC 1 cut(s) 314
PflMI CCANNNNNTGG 1 cut(s) 273
PkrI GCNGC 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 402
PspGI CCWGG 1 cut(s) 402
PspPI GGNCC 2 cut(s) 190, 400
PstNI CAGNNNCTG 1 cut(s) 255
PsyI GACNNNGTC 1 cut(s) 314
SacI GAGCTC 1 cut(s) 216
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 2 cut(s) 75, 144
Sau96I GGNCC 2 cut(s) 190, 400
ScrFI CCNGG 1 cut(s) 404
SduI GDGCHC 1 cut(s) 216
SetI ASST 5 cut(s) 30, 159, 205, 216, 257
SfaNI GCATC 1 cut(s) 253
SinI GGWCC 1 cut(s) 400
Sse9I AATT 5 cut(s) 8, 132, 161, 320, 334
SsiI CCGC 1 cut(s) 392
SspMI CTAG 1 cut(s) 194
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 223, 314
TaqI TCGA 1 cut(s) 372
TasI AATT 5 cut(s) 8, 132, 161, 320, 334
TscAI CASTG 2 cut(s) 54, 95
TseI GCWGC 1 cut(s) 255
TspDTI ATGAA 2 cut(s) 56, 213
TspRI CASTG 2 cut(s) 54, 95
Tth111I GACNNNGTC 1 cut(s) 314
Van91I CCANNNNNTGG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 400
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.