pycom07g16640

DNL-type zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015054
Physical Location & Seq
Reverse (-)
19033400 .. 19035594
2195 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 705 bp
ATGTCAATCTCAAAGACCAAGAATTATAATCCAAAGACATTTAAAACTTGGAGGGCACACTGTCTCTGCGGCGTCGCCTACATAATACCGTTTACAGAAACCGACCTCGAACCGACAAGCTTAAGCAATCCAAGCAAGAACTCAACCATGGCGGCTACGATGTTGCAGAGGCGCTTAGGCTCACTCCTTCTCCGCAATCAGCAGCCTAACTCCCATCTCTTCAAAGATCAATTTGTTCCTTCTGCAAGTTCTATACTCAGAAGATATGGATTTGACAAAAGAGATCTTCAAACGCAAACAAATCCAACCAATCTGGTTAATGAGGATCTGGCAAATCTTGAAGATGGCAGTTCGAAACCCAATAATGATGCGAACGCTATCAACAGTTCCGATGCCAAATTTTCTGCTTCTTCCGAGCAGAAAATATCCCCAAGGCATGACCTGGCCATGCTTTTTACCTGCACTGTCTGTGAAACAAGATCCATGAAGACGTGCAGTAGAGAATCATACGAGAAAGGTGTGGTGGTAGCTCGCTGTGGCGGGTGTAACAATCTCGACCTGATTGTCGATCATCTCGGGTATTTTGGAAAACCATCCAACGTTGAGCAATTTCTGGCTGCCCGTGGGGAAGAGGTGAAGAAAGGTTCTGCTGATACGTTGAATCTGACTCTCGAAGACATAGCCGGAATGAAACCTCAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-DNL

Protein Analysis

235

Amino Acids

25.83

Weight (kDa)

8.43

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-DNL PF05180 150 - 210 9e-22 DNL zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54826
fragaria_vesca FvH4_7g19000
malus_domestica MD01G1105600.v1.1 MD07G1171800.v1.1
pyrus_communis pycom01g13240 pycom07g16640
rosa_chinensis RchiOBHm_Chr1g0362931
rosa_laevigata RLG00000027611
rosa_multiflora Rmu_sc0001502.1_g000005
rosa_roxburghii Rroxscaffold_4G00293640
rosa_rugosa Rorug01G0302200
rosa_samantha Rh1AG311700 Rh1BG274800 Rh1CG292000 Rh1DG305500
rosa_wichuraiana Rw1G027620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 27
AasI GACNNNNNNGTC 1 cut(s) 563
Acc36I ACCTGC 1 cut(s) 467
AciI CCGC 4 cut(s) 69, 152, 193, 540
AclI AACGTT 1 cut(s) 600
AclWI GGATC 2 cut(s) 333, 474
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 1 cut(s) 398
AcyI GRCGYC 1 cut(s) 72
AflII CTTAAG 1 cut(s) 121
AgsI TTSAA 4 cut(s) 223, 290, 341, 661
AjiI CACGTC 1 cut(s) 492
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 2 cut(s) 120, 530
AluI AGCT 2 cut(s) 120, 530
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 2 cut(s) 333, 474
Ama87I CYCGRG 1 cut(s) 575
AoxI GGCC 1 cut(s) 444
ApeKI GCWGC 2 cut(s) 202, 617
ApoI RAATTY 1 cut(s) 398
AspLEI GCGC 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 646
AsuII TTCGAA 1 cut(s) 353
AvaI CYCGRG 1 cut(s) 575
AxyI CCTNAGG 1 cut(s) 696
BaeGI GKGCMC 1 cut(s) 58
BalI TGGCCA 1 cut(s) 446
BbsI GAAGAC 2 cut(s) 494, 681
BbvI GCAGC 2 cut(s) 214, 604
BccI CCATC 3 cut(s) 222, 338, 601
BciT130I CCWGG 1 cut(s) 443
BcoDI GTCTC 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 175
BfrI CTTAAG 1 cut(s) 121
BfuAI ACCTGC 1 cut(s) 467
BglII AGATCT 1 cut(s) 283
BisI GCNGC 4 cut(s) 70, 153, 203, 618
BlsI GCNGC 4 cut(s) 71, 154, 204, 619
Bme1390I CCNGG 1 cut(s) 443
BmeT110I CYCGRG 1 cut(s) 575
BmgBI CACGTC 1 cut(s) 492
BmrFI CCNGG 1 cut(s) 443
BmsI GCATC 2 cut(s) 358, 382
BpiI GAAGAC 2 cut(s) 494, 681
Bpu10I CCTNAGC 1 cut(s) 175
Bpu14I TTCGAA 1 cut(s) 353
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 3 cut(s) 147, 431, 622
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bse21I CCTNAGG 1 cut(s) 696
BseBI CCWGG 1 cut(s) 443
BseDI CCNNGG 3 cut(s) 147, 431, 622
BseGI GGATG 1 cut(s) 593
BseMII CTCAG 1 cut(s) 271
BseSI GKGCMC 1 cut(s) 58
BseXI GCAGC 2 cut(s) 214, 604
BsgI GTGCAG 2 cut(s) 445, 514
BshFI GGCC 1 cut(s) 446
BsiHKCI CYCGRG 1 cut(s) 575
BsiSI CCGG 1 cut(s) 684
BsmAI GTCTC 1 cut(s) 68
BsnI GGCC 1 cut(s) 446
BsoBI CYCGRG 1 cut(s) 575
Bsp119I TTCGAA 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 58
Bsp143I GATC 5 cut(s) 226, 283, 325, 479, 568
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 4 cut(s) 69, 152, 193, 540
BspANI GGCC 1 cut(s) 446
BspCNI CTCAG 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 467
BspPI GGATC 2 cut(s) 333, 474
BspT104I TTCGAA 1 cut(s) 353
BspTI CTTAAG 1 cut(s) 121
BssECI CCNNGG 3 cut(s) 147, 431, 622
BssMI GATC 5 cut(s) 226, 283, 325, 479, 568
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 147, 431
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 4 cut(s) 62, 90, 386, 466
Bst6I CTCTTC 2 cut(s) 224, 624
BstACI GRCGYC 1 cut(s) 72
BstAFI CTTAAG 1 cut(s) 121
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 3 cut(s) 175, 257, 696
BstDSI CCRYGG 2 cut(s) 147, 622
BstF5I GGATG 1 cut(s) 593
BstH2I RGCGCY 1 cut(s) 175
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 5 cut(s) 229, 286, 328, 482, 571
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 5 cut(s) 226, 283, 325, 479, 568
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 1 cut(s) 443
BstSCI CCNGG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 58
BstV1I GCAGC 2 cut(s) 214, 604
BstV2I GAAGAC 2 cut(s) 494, 681
BstX2I RGATCY 3 cut(s) 283, 325, 479
BstYI RGATCY 3 cut(s) 283, 325, 479
Bsu36I CCTNAGG 1 cut(s) 696
BsuRI GGCC 1 cut(s) 446
BtgI CCRYGG 2 cut(s) 147, 622
BtrI CACGTC 1 cut(s) 492
BtsCI GGATG 1 cut(s) 593
BtsIMutI CAGTG 2 cut(s) 58, 462
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 1 cut(s) 532
CfoI GCGC 1 cut(s) 174
CseI GACGC 1 cut(s) 61
CviAII CATG 4 cut(s) 148, 437, 448, 484
CviJI RGCY 8 cut(s) 120, 155, 180, 205, 446, 530, 617, 683
CviKI_1 RGCY 8 cut(s) 120, 155, 180, 205, 446, 530, 617, 683
DdeI CTNAG 3 cut(s) 175, 257, 696
DpnI GATC 5 cut(s) 228, 285, 327, 481, 570
DpnII GATC 5 cut(s) 226, 283, 325, 479, 568
DraI TTTAAA 1 cut(s) 43
DrdI GACNNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 563
EaeI YGGCCR 1 cut(s) 444
Eam1104I CTCTTC 2 cut(s) 224, 624
EarI CTCTTC 2 cut(s) 224, 624
Eco130I CCWWGG 2 cut(s) 147, 431
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 575
EcoRII CCWGG 1 cut(s) 441
EcoT14I CCWWGG 2 cut(s) 147, 431
ErhI CCWWGG 2 cut(s) 147, 431
FaeI CATG 4 cut(s) 151, 440, 451, 487
FatI CATG 4 cut(s) 147, 436, 447, 483
FauI CCCGC 1 cut(s) 533
Fnu4HI GCNGC 4 cut(s) 70, 153, 203, 618
FokI GGATG 1 cut(s) 580
Fsp4HI GCNGC 4 cut(s) 70, 153, 203, 618
GlaI GCGC 1 cut(s) 173
GluI GCNGC 4 cut(s) 70, 153, 203, 618
HaeII RGCGCY 1 cut(s) 175
HaeIII GGCC 1 cut(s) 446
HapII CCGG 1 cut(s) 684
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 174
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 4 cut(s) 151, 440, 451, 487
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 118
HinfI GANTC 3 cut(s) 503, 661, 667
HpaII CCGG 1 cut(s) 684
HphI GGTGA 1 cut(s) 646
Hpy166II GTNNAC 1 cut(s) 93
Hpy188I TCNGA 4 cut(s) 260, 391, 415, 666
Hpy188III TCNNGA 3 cut(s) 338, 554, 671
Hpy8I GTNNAC 1 cut(s) 93
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 197, 249
HpyCH4III ACNGT 4 cut(s) 62, 90, 386, 466
HpyCH4IV ACGT 3 cut(s) 491, 600, 656
HpyCH4V TGCA 4 cut(s) 166, 245, 462, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 3 cut(s) 175, 257, 696
HpySE526I ACGT 3 cut(s) 491, 600, 656
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 4 cut(s) 151, 440, 451, 487
HspAI GCGC 1 cut(s) 172
Kzo9I GATC 5 cut(s) 226, 283, 325, 479, 568
LpnPI CCDG 9 cut(s) 299, 314, 428, 455, 472, 572, 599, 683, 697
Lsp1109I GCAGC 2 cut(s) 214, 604
LweI GCATC 2 cut(s) 358, 382
MaeII ACGT 3 cut(s) 491, 600, 656
MaeIII GTNAC 1 cut(s) 545
MalI GATC 5 cut(s) 228, 285, 327, 481, 570
MboI GATC 5 cut(s) 226, 283, 325, 479, 568
MboII GAAGA 9 cut(s) 211, 273, 278, 353, 402, 499, 641, 649, 686
MflI RGATCY 3 cut(s) 283, 325, 479
MhlI GDGCHC 1 cut(s) 58
MlsI TGGCCA 1 cut(s) 446
MluCI AATT 4 cut(s) 22, 230, 398, 608
MluNI TGGCCA 1 cut(s) 446
MlyI GAGTC 1 cut(s) 661
MmeI TCCRAC 2 cut(s) 329, 621
MnlI CCTC 6 cut(s) 45, 116, 162, 316, 625, 705
Mox20I TGGCCA 1 cut(s) 446
MscI TGGCCA 1 cut(s) 446
MseI TTAA 3 cut(s) 42, 122, 318
Msp20I TGGCCA 1 cut(s) 446
MspCI CTTAAG 1 cut(s) 121
MspI CCGG 1 cut(s) 684
MspR9I CCNGG 1 cut(s) 443
MvaI CCWGG 1 cut(s) 443
MwoI GCNNNNNNNGC 1 cut(s) 132
NcoI CCATGG 1 cut(s) 147
NdeII GATC 5 cut(s) 226, 283, 325, 479, 568
NlaIII CATG 4 cut(s) 151, 440, 451, 487
NspV TTCGAA 1 cut(s) 353
PfeI GAWTC 2 cut(s) 503, 661
PkrI GCNGC 4 cut(s) 71, 154, 204, 619
PleI GAGTC 1 cut(s) 661
PpsI GAGTC 1 cut(s) 661
PsiI TTATAA 1 cut(s) 27
Psp1406I AACGTT 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 441
PspGI CCWGG 1 cut(s) 441
PsuI RGATCY 3 cut(s) 283, 325, 479
SaqAI TTAA 3 cut(s) 42, 122, 318
SatI GCNGC 4 cut(s) 70, 153, 203, 618
Sau3AI GATC 5 cut(s) 226, 283, 325, 479, 568
SchI GAGTC 1 cut(s) 661
ScrFI CCNGG 1 cut(s) 443
SduI GDGCHC 1 cut(s) 58
SfaNI GCATC 2 cut(s) 358, 382
SfuI TTCGAA 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 4 cut(s) 22, 230, 398, 608
SsiI CCGC 4 cut(s) 69, 152, 193, 540
StyD4I CCNGG 1 cut(s) 441
StyI CCWWGG 2 cut(s) 147, 431
TaaI ACNGT 4 cut(s) 62, 90, 386, 466
TaiI ACGT 3 cut(s) 494, 603, 659
TaqI TCGA 5 cut(s) 108, 353, 555, 567, 672
TasI AATT 4 cut(s) 22, 230, 398, 608
TauI GCSGC 2 cut(s) 72, 155
TfiI GAWTC 2 cut(s) 503, 661
Tru1I TTAA 3 cut(s) 42, 122, 318
Tru9I TTAA 3 cut(s) 42, 122, 318
TscAI CASTG 2 cut(s) 65, 469
TseI GCWGC 2 cut(s) 202, 617
TspDTI ATGAA 2 cut(s) 500, 704
TspRI CASTG 2 cut(s) 65, 469
Vha464I CTTAAG 1 cut(s) 121
XapI RAATTY 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.