pycom07g16870

Endoplasmic reticulum metallopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0011223
Physical Location & Seq
Reverse (-)
19358163 .. 19363878
5716 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2946 bp
ATGAGCACCATAAGAGCAATTCTAATTGAATTATTATCATCAAATTTAAATCATATAGGATTTATCCGATGTTCCAGAGAAATCGAAATCTCAGTCCACTTACAAGTGTTGATAAAGCAAAAGCCACGATGGTCTGACTTTTTAGATTTATTCAGACTGCAACCAATATCCAATCACAGTTTTTCCTACTTTCTCCGACGACACTTCGCAATGCGACGGAGGCCCCAGCAGACTCCGGCACCCGCCACCGCCAATCCATCACCCACCGGAGCAGAACCAGTTTCCACTTCATGGGTCGGACAGCAGAGGCCTCGGCGGTCACCATTCGTTTGGCTCACCTTGTTCCTGGCCATCGGATACTGTTCTTATGGAGTTTACCAATACCAGTTTGAGAGCTTGCCCGAGCCGCTCACGGCGGACCAGGCCGGAAAGCGCGGCTTCTCTGAGTTTTCAGCTCTCAAACACGTCGAGGCCTTGACCCAATTGGGCCCTCACTCTGTCGGCTCCAATGCTCTCAACCTAGCTCTCCAGTATGTTTTGGCAGAAGCTGAGAAGATCAAGAAAACTGCTCACTGGGAGGTTGATGTTGAAGTGGACTCGTTCCATGTCGAATCGGGAGCAAATCGAATGGTTGGTGGCCTGTTCAAGGGAAGAACACTTGTATATGCGGATCTAAATCATATTGTTGTAAGAGTTTTGCCAAAGTATGCGCCTGAATCAGTGGATAATGCGATTCTGGTCTCTTCCCACATCGACACCGTCTTCTCAACGGGAGGGGCTGGAGATTGCAGTTCGTGTGTAGCCGTCATGTTGGAACTTGCTCGTGGGGTTTCCCAGTGGGCTCATGGATTTAAGCATGCTGTTATATTTTTGTTTAATACTGGGGAGGAGGAGGGTCTAAATGGTGCTCATAGCTTTATAACTCAGCATCCGTGGAGTACAACTATTCGTCTGGCTGTTGATTTGGAGGCTATGGGTATTGGAGGGAAGTCTGGCATATTTCAGGCTGATGCTTGGTCTATTGAGAATTTTGCCTCGGTAGCAAAATACCCGTCTGGCCAAATTATAGGACAGGATATATTTTCTTCTGGAGCCATAAAATCTGCAACAGATTTCCAAGTATATAAGGAGGTTGCGGGCCTTCCAGGGCTCGACTTCGCGTACACAGATAACACTGCAGTATACCATACAAAGAATGACAAAATCGAGCTTCATACATTAGGATCTCTTCAGCATCTTGGAGAAAATATGCTTGCTTTTCTGCTAAAGATTGCTGCTTCCTCTCATCTTCCAAAAGCCAGTGCAGTGGTAGAAGAGAATAAGGGCGAGACTGCTGCCATATATTTTGACATATTGGGGTCGTATATGATCGTGTATCGTCAACGCTTTGCAAATATGCTTCATAATTCAGTGATAGCGCAATCACTTCTGATATGGACTACATCATTGCTTATGGGTGGTTATCCGGCTGCAATTTCATTGGCCTTGTCATGTTTGAGTATCATATTAATGTGGATATTCGCAATAAGTTTTGCTCTTCTTCCTGCCTTCATTATATCACTAATATCTTCTTCACCAGTGCCATATGTTGCAAACCCATGGCTAGTAATCGGGTTATTTGGCGCACCTGCTCTTATTGGGGCGTTAAGTGGTCAATATCTGGGTTATCTTGGTCTGCATACATATTTGTCAAAAGTATACGCCAAGAAAAAACAACTCTCACCTGATATACAAGCTGGTTTGGTTAAATTAGAGGCTGAAAGATGGCTCTACAAATCTGGATCTCTTCAGTGGCTCATTCTCCTCATTTTGGGAACCTATTACAAAATTGGATCAACTTATTTGGCTCTTGCTTGGTTGGTCCCCCCAGCATTCGCATATGGCTTTCTTGAAGCGACTCTAACCCCAGCTCGATTCCCCAAGCCACTCAGACTTGCAACCCTGCTGATAGGATTAGCTGTACCAATAGTAATTTCTTCCGGCGGATTTATTCGGCTGGCTGGAATGGTAATTGGGACTGTGGTTCGACTTGACAGGAACCCAGGTGGCACCCCCGAGTGGCTAGCGAATGTTATACTTGCTGTTTTTGTTGCTATTGTTGTGTGCCTGACTTTGGTGTATCTCCTGTCATATATTCATCTTTCAGGTGCCAAGAGATCGATTGTCCTTTCAACTTGCATGCTGTTCGGCCTCTCACTTGCTGTTGTATTGTTGGGTATCGTTCCACCATTTACCGCAGACACATCCAGAGCTGTAAATGTTGTGCATGTTGTAGATACGACAGGAAGCGTTGATGGAAACCAAGATCCTAGATCATATGTGTCTTTGTTTTCTTTCACTCCTGGAAAGTTGACAGAGGAGGCTGAGCAAATTAATGAAGGTTTCAGATGTGGTAGAGACAAAGTTGTTGATCTTGTAACCTTTTCAGCCAAGTACAGTTGTTGGACTTATGATGACAGTGACAGTGGATGGAGCAAATCAGATATACCCACAATGAACGTTGGTAGCGATACTCATGGTAATGAAAGAACAACACGAGTACTACTTGATACAAAAGGTTCAACACGCTGGACGCTTGCTATCAATGCTGGTGACATAGAAGATTTCACATTCAAAGTTGCAGGAAGCTCGGAGGAATTGGCTTCTTTGGGTGACATGAGTAGCGTAGATGGATGGCACGTTATCCAGTATTCCGGAGGACAAAATGCACCAACAAGGTTCGATCTAGCACTCTTCTGGTCTCAAAACTCTACCCGGCTGGCTCACGATGTTGAAGGGAAGAGAGACGAGCACGCTCCCCTTCTAAAGTTAAGAACTGATATGGACATAGTGACTCCTAAAGTCGAAAGAGTTCTTTCGAAGCTTCCTCCTTGGTGTTCGCTGTTTGGCAAGTCGACGTCCCCCCACACCCTTGCATTTTTGAGCAATCTTCCTGTTAATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M28

Protein Analysis

982

Amino Acids

107.42

Weight (kDa)

6.98

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M28 PF04389 233 - 420 2e-44 Peptidase family M28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20660 AT5G20660 AT5G20660 AT5G20660
fragaria_vesca FvH4_7g19460 FvH4_7g19460 FvH4_7g19460 FvH4_7g19460
malus_domestica MD07G1174500.v1.1
prunus_persica Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1
pyrus_communis pycom07g16870
rosa_chinensis RchiOBHm_Chr1g0363331
rosa_laevigata RLG00000027573
rosa_roxburghii Rroxscaffold_4G00293270
rosa_rugosa Rorug01G0306400
rosa_samantha Rh1AG315200 Rh1CG294900
rosa_wichuraiana Rw1G027930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 920
AarI CACCTGC 1 cut(s) 1636
AatII GACGTC 1 cut(s) 2900
Acc36I ACCTGC 1 cut(s) 1636
AccB1I GGYRCC 3 cut(s) 238, 2048, 2147
AccB7I CCANNNNNTGG 1 cut(s) 291
AccBSI CCGCTC 1 cut(s) 409
AccI GTMKAC 3 cut(s) 1182, 1698, 2894
AccII CGCG 2 cut(s) 435, 1160
AccIII TCCGGA 1 cut(s) 2693
AclI AACGTT 1 cut(s) 2499
AclWI GGATC 5 cut(s) 678, 1231, 1789, 1840, 2300
AcoI YGGCCR 2 cut(s) 348, 1057
AcsI RAATTY 2 cut(s) 43, 1027
AcuI CTGAAG 2 cut(s) 1214, 1772
AcyI GRCGYC 1 cut(s) 2897
AfaI GTAC 5 cut(s) 940, 1163, 1962, 2435, 2541
AfiI CCNNNNNNNGG 5 cut(s) 291, 646, 1810, 2058, 2113
AflIII ACRYGT 1 cut(s) 463
AgsI TTSAA 8 cut(s) 29, 590, 646, 1892, 2172, 2562, 2614, 2774
AjiI CACGTC 1 cut(s) 466
AjnI CCWGG 5 cut(s) 345, 420, 1144, 2041, 2341
AjuI GAANNNNNNNTTGG 2 cut(s) 1957, 1989
Alw21I GWGCWC 3 cut(s) 8, 910, 2793
Alw26I GTCTC 5 cut(s) 745, 1322, 2391, 2745, 2778
AlwI GGATC 5 cut(s) 678, 1231, 1789, 1840, 2300
AlwNI CAGNNNCTG 1 cut(s) 548
Ama87I CYCGRG 2 cut(s) 401, 2054
Aor13HI TCCGGA 1 cut(s) 2693
ApaI GGGCCC 1 cut(s) 491
ApeKI GCWGC 3 cut(s) 1274, 1334, 1469
ApoI RAATTY 2 cut(s) 43, 1027
ArsI GACNNNNNNTTYG 4 cut(s) 78, 110, 1037, 1069
AseI ATTAAT 2 cut(s) 1508, 2373
Asp700I GAANNNNTTC 1 cut(s) 2850
AspLEI GCGC 4 cut(s) 435, 712, 1420, 1625
AspS9I GGNCC 6 cut(s) 222, 418, 487, 488, 1138, 1861
AsuC2I CCSGG 1 cut(s) 2755
AsuHPI GGTGA 7 cut(s) 252, 312, 328, 1566, 1713, 2603, 2663
AsuII TTCGAA 1 cut(s) 2858
AsuNHI GCTAGC 1 cut(s) 2062
AvaI CYCGRG 2 cut(s) 401, 2054
AvaII GGWCC 2 cut(s) 418, 1861
BaeGI GKGCMC 1 cut(s) 491
BalI TGGCCA 2 cut(s) 350, 1059
BanI GGYRCC 3 cut(s) 238, 2048, 2147
BanII GRGCYC 3 cut(s) 491, 844, 1152
BauI CACGAG 2 cut(s) 822, 2535
BbsI GAAGAC 1 cut(s) 754
Bbv12I GWGCWC 3 cut(s) 8, 910, 2793
BbvI GCAGC 3 cut(s) 1261, 1321, 1456
BccI CCATC 8 cut(s) 123, 265, 359, 1758, 2288, 2463, 2663, 2667
BceAI ACGGC 2 cut(s) 429, 788
BcgI CGANNNNNNTGC 8 cut(s) 293, 327, 1316, 1350, 2167, 2201, 2610, 2644
BciT130I CCWGG 5 cut(s) 347, 422, 1146, 2043, 2343
BciVI GTATCC 1 cut(s) 350
BcnI CCSGG 1 cut(s) 2755
BcoDI GTCTC 5 cut(s) 745, 1322, 2391, 2745, 2778
BfaI CTAG 5 cut(s) 521, 1604, 2063, 2310, 2726
BfmI CTRYAG 1 cut(s) 1176
BfuAI ACCTGC 1 cut(s) 1636
BfuI GTATCC 1 cut(s) 350
BisI GCNGC 5 cut(s) 407, 436, 1275, 1335, 1470
BlpI GCTNAGC 1 cut(s) 2364
BlsI GCNGC 5 cut(s) 408, 437, 1276, 1336, 1471
BmcAI AGTACT 1 cut(s) 2541
Bme1390I CCNGG 6 cut(s) 347, 422, 1146, 2043, 2343, 2755
Bme18I GGWCC 2 cut(s) 418, 1861
BmeT110I CYCGRG 2 cut(s) 401, 2054
BmgBI CACGTC 1 cut(s) 466
BmgT120I GGNCC 6 cut(s) 222, 418, 487, 488, 1138, 1861
BmrFI CCNGG 6 cut(s) 347, 422, 1146, 2043, 2343, 2755
BmrI ACTGGG 3 cut(s) 583, 829, 891
BmsI GCATC 3 cut(s) 937, 1000, 1243
BmtI GCTAGC 1 cut(s) 2066
BmuI ACTGGG 3 cut(s) 583, 829, 891
BpiI GAAGAC 1 cut(s) 754
BpmI CTGGAG 3 cut(s) 512, 801, 1110
Bpu1102I GCTNAGC 1 cut(s) 2364
Bpu14I TTCGAA 1 cut(s) 2858
BpuMI CCSGG 1 cut(s) 2755
Bsa29I ATCGAT 1 cut(s) 2159
BsaBI GATNNNNATC 1 cut(s) 675
BsaHI GRCGYC 1 cut(s) 2897
BsaI GGTCTC 2 cut(s) 745, 2745
BsaJI CCNNGG 7 cut(s) 311, 932, 1035, 1145, 1598, 2041, 2870
BsaWI WCCGGW 2 cut(s) 266, 2693
Bsc4I CCNNNNNNNGG 5 cut(s) 291, 646, 1810, 2058, 2113
Bse1I ACTGG 9 cut(s) 278, 385, 529, 578, 835, 886, 1299, 1577, 2686
Bse3DI GCAATG 2 cut(s) 216, 1445
Bse8I GATNNNNATC 1 cut(s) 675
BseAI TCCGGA 1 cut(s) 2693
BseBI CCWGG 5 cut(s) 347, 422, 1146, 2043, 2343
BseCI ATCGAT 1 cut(s) 2159
BseDI CCNNGG 7 cut(s) 311, 932, 1035, 1145, 1598, 2041, 2870
BseGI GGATG 4 cut(s) 928, 2243, 2474, 2678
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 5 cut(s) 291, 646, 1810, 2058, 2113
BseMI GCAATG 2 cut(s) 216, 1445
BseMII CTCAG 6 cut(s) 105, 435, 540, 938, 1942, 2355
BseNI ACTGG 9 cut(s) 278, 385, 529, 578, 835, 886, 1299, 1577, 2686
BseRI GAGGAG 4 cut(s) 902, 905, 1793, 2372
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 3 cut(s) 1261, 1321, 1456
BseYI CCCAGC 3 cut(s) 225, 1867, 1906
BsgI GTGCAG 1 cut(s) 1323
Bsh1236I CGCG 2 cut(s) 435, 1160
BshNI GGYRCC 3 cut(s) 238, 2048, 2147
BshVI ATCGAT 1 cut(s) 2159
BsiHKAI GWGCWC 3 cut(s) 8, 910, 2793
BsiHKCI CYCGRG 2 cut(s) 401, 2054
BsiSI CCGG 7 cut(s) 236, 267, 426, 1466, 1980, 2694, 2755
BslFI GGGAC 3 cut(s) 1847, 2029, 2884
BslI CCNNNNNNNGG 5 cut(s) 291, 646, 1810, 2058, 2113
BsmAI GTCTC 5 cut(s) 745, 1322, 2391, 2745, 2778
BsmBI CGTCTC 1 cut(s) 2778
BsmFI GGGAC 3 cut(s) 1847, 2029, 2884
BsmI GAATGC 1 cut(s) 1871
Bso31I GGTCTC 2 cut(s) 745, 2745
BsoBI CYCGRG 2 cut(s) 401, 2054
Bsp119I TTCGAA 1 cut(s) 2858
Bsp120I GGGCCC 1 cut(s) 487
Bsp1286I GDGCHC 6 cut(s) 8, 491, 844, 910, 1152, 2793
Bsp13I TCCGGA 1 cut(s) 2693
Bsp1720I GCTNAGC 1 cut(s) 2364
Bsp19I CCATGG 1 cut(s) 1598
BspCNI CTCAG 6 cut(s) 104, 436, 541, 937, 1941, 2356
BspDI ATCGAT 1 cut(s) 2159
BspEI TCCGGA 1 cut(s) 2693
BspFNI CGCG 2 cut(s) 435, 1160
BspMAI CTGCAG 1 cut(s) 1180
BspMI ACCTGC 1 cut(s) 1636
BspOI GCTAGC 1 cut(s) 2066
BspPI GGATC 5 cut(s) 678, 1231, 1789, 1840, 2300
BspQI GCTCTTC 1 cut(s) 1542
BspT104I TTCGAA 1 cut(s) 2858
BspT107I GGYRCC 3 cut(s) 238, 2048, 2147
BspTNI GGTCTC 2 cut(s) 745, 2745
BsrBI CCGCTC 1 cut(s) 409
BsrDI GCAATG 2 cut(s) 216, 1445
BsrI ACTGG 9 cut(s) 278, 385, 529, 578, 835, 886, 1299, 1577, 2686
BssECI CCNNGG 7 cut(s) 311, 932, 1035, 1145, 1598, 2041, 2870
BssNAI GTATAC 2 cut(s) 1183, 1699
BssNI GRCGYC 1 cut(s) 2897
BssSI CACGAG 2 cut(s) 822, 2535
BssT1I CCWWGG 2 cut(s) 1598, 2870
Bst1107I GTATAC 2 cut(s) 1183, 1699
Bst2BI CACGAG 2 cut(s) 822, 2535
Bst2UI CCWGG 5 cut(s) 347, 422, 1146, 2043, 2343
Bst4CI ACNGT 7 cut(s) 179, 362, 760, 2020, 2438, 2459, 2465
Bst6I CTCTTC 7 cut(s) 748, 1233, 1308, 1542, 1791, 2738, 2774
BstACI GRCGYC 1 cut(s) 2897
BstBI TTCGAA 1 cut(s) 2858
BstDEI CTNAG 6 cut(s) 91, 444, 549, 924, 1928, 2364
BstDSI CCRYGG 2 cut(s) 932, 1598
BstEII GGTNACC 1 cut(s) 318
BstENI CCTNNNNNAGG 1 cut(s) 644
BstF5I GGATG 4 cut(s) 928, 2243, 2474, 2678
BstFNI CGCG 2 cut(s) 435, 1160
BstHHI GCGC 4 cut(s) 435, 712, 1420, 1625
BstMAI GTCTC 5 cut(s) 745, 1322, 2391, 2745, 2778
BstMWI GCNNNNNNNGC 5 cut(s) 220, 406, 422, 1040, 2585
BstNI CCWGG 5 cut(s) 347, 422, 1146, 2043, 2343
BstNSI RCATGY 3 cut(s) 860, 2182, 2270
BstPI GGTNACC 1 cut(s) 318
BstSCI CCNGG 6 cut(s) 345, 420, 1144, 2041, 2341, 2753
BstSFI CTRYAG 1 cut(s) 1176
BstSLI GKGCMC 1 cut(s) 491
BstUI CGCG 2 cut(s) 435, 1160
BstV1I GCAGC 3 cut(s) 1261, 1321, 1456
BstV2I GAAGAC 1 cut(s) 754
BstX2I RGATCY 4 cut(s) 670, 1223, 1781, 2305
BstXI CCANNNNNNTGG 2 cut(s) 330, 1306
BstYI RGATCY 4 cut(s) 670, 1223, 1781, 2305
BstZ17I GTATAC 2 cut(s) 1183, 1699
Bsu15I ATCGAT 1 cut(s) 2159
BsuI GTATCC 1 cut(s) 350
BsuTUI ATCGAT 1 cut(s) 2159
BtgI CCRYGG 2 cut(s) 932, 1598
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 4 cut(s) 928, 2243, 2474, 2678
BtsI GCAGTG 2 cut(s) 1173, 1311
BveI ACCTGC 1 cut(s) 1636
CaiI CAGNNNCTG 1 cut(s) 548
CfoI GCGC 4 cut(s) 435, 712, 1420, 1625
Cfr13I GGNCC 6 cut(s) 222, 418, 487, 488, 1138, 1861
ClaI ATCGAT 1 cut(s) 2159
CseI GACGC 1 cut(s) 2581
Csp6I GTAC 5 cut(s) 939, 1162, 1961, 2434, 2540
CviQI GTAC 5 cut(s) 939, 1162, 1961, 2434, 2540
DdeI CTNAG 6 cut(s) 91, 444, 549, 924, 1928, 2364
DraI TTTAAA 1 cut(s) 48
EaeI YGGCCR 2 cut(s) 348, 1057
Eam1104I CTCTTC 7 cut(s) 748, 1233, 1308, 1542, 1791, 2738, 2774
EarI CTCTTC 7 cut(s) 748, 1233, 1308, 1542, 1791, 2738, 2774
EciI GGCGGA 2 cut(s) 431, 1998
Eco130I CCWWGG 2 cut(s) 1598, 2870
Eco147I AGGCCT 2 cut(s) 310, 473
Eco24I GRGCYC 3 cut(s) 491, 844, 1152
Eco31I GGTCTC 2 cut(s) 745, 2745
Eco47I GGWCC 2 cut(s) 418, 1861
Eco57I CTGAAG 2 cut(s) 1214, 1772
Eco88I CYCGRG 2 cut(s) 401, 2054
Eco91I GGTNACC 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 644
EcoO109I RGGNCCY 2 cut(s) 222, 488
EcoO65I GGTNACC 1 cut(s) 318
EcoRII CCWGG 5 cut(s) 345, 420, 1144, 2041, 2341
EcoT14I CCWWGG 2 cut(s) 1598, 2870
EcoT38I GRGCYC 3 cut(s) 491, 844, 1152
ErhI CCWWGG 2 cut(s) 1598, 2870
Esp3I CGTCTC 1 cut(s) 2778
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FaqI GGGAC 3 cut(s) 1847, 2029, 2884
FauI CCCGC 2 cut(s) 250, 1129
FauNDI CATATG 3 cut(s) 1585, 1879, 2317
FblI GTMKAC 3 cut(s) 1182, 1698, 2894
Fnu4HI GCNGC 5 cut(s) 407, 436, 1275, 1335, 1470
FokI GGATG 4 cut(s) 915, 2230, 2481, 2685
FriOI GRGCYC 3 cut(s) 491, 844, 1152
Fsp4HI GCNGC 5 cut(s) 407, 436, 1275, 1335, 1470
FspBI CTAG 5 cut(s) 521, 1604, 2063, 2310, 2726
GlaI GCGC 4 cut(s) 434, 711, 1419, 1624
GluI GCNGC 5 cut(s) 407, 436, 1275, 1335, 1470
GsaI CCCAGC 3 cut(s) 229, 1871, 1910
GsuI CTGGAG 3 cut(s) 512, 801, 1110
HapII CCGG 7 cut(s) 236, 267, 426, 1466, 1980, 2694, 2755
HgaI GACGC 1 cut(s) 2581
HhaI GCGC 4 cut(s) 435, 712, 1420, 1625
Hin1I GRCGYC 1 cut(s) 2897
Hin6I GCGC 4 cut(s) 433, 710, 1418, 1623
HinP1I GCGC 4 cut(s) 433, 710, 1418, 1623
HincII GTYRAC 3 cut(s) 1382, 2352, 2895
HindII GTYRAC 3 cut(s) 1382, 2352, 2895
HindIII AAGCTT 1 cut(s) 2861
HinfI GANTC 8 cut(s) 232, 596, 611, 716, 733, 1897, 1914, 2833
HpaII CCGG 7 cut(s) 236, 267, 426, 1466, 1980, 2694, 2755
HphI GGTGA 7 cut(s) 252, 312, 328, 1566, 1713, 2603, 2663
Hpy166II GTNNAC 9 cut(s) 97, 376, 595, 1164, 1183, 1382, 1699, 2352, 2895
Hpy188III TCNNGA 9 cut(s) 75, 559, 615, 1089, 1779, 1889, 2247, 2694, 2765
Hpy8I GTNNAC 9 cut(s) 97, 376, 595, 1164, 1183, 1382, 1699, 2352, 2895
Hpy99I CGWCG 4 cut(s) 201, 219, 470, 2899
HpyAV CCTTC 5 cut(s) 1151, 1558, 2372, 2768, 2810
HpyCH4III ACNGT 7 cut(s) 179, 362, 760, 2020, 2438, 2459, 2465
HpyCH4IV ACGT 4 cut(s) 465, 2499, 2679, 2897
HpyF10VI GCNNNNNNNGC 5 cut(s) 220, 406, 422, 1040, 2585
HpyF3I CTNAG 6 cut(s) 91, 444, 549, 924, 1928, 2364
HpySE526I ACGT 4 cut(s) 465, 2499, 2679, 2897
Hsp92I GRCGYC 1 cut(s) 2897
HspAI GCGC 4 cut(s) 433, 710, 1418, 1623
Kpn2I TCCGGA 1 cut(s) 2693
LguI GCTCTTC 1 cut(s) 1542
LmnI GCTCC 6 cut(s) 269, 509, 617, 1091, 2472, 2800
Lsp1109I GCAGC 3 cut(s) 1261, 1321, 1456
LweI GCATC 3 cut(s) 937, 1000, 1243
MaeI CTAG 5 cut(s) 521, 1604, 2063, 2310, 2726
MaeII ACGT 4 cut(s) 465, 2499, 2679, 2897
MaeIII GTNAC 6 cut(s) 318, 2416, 2459, 2591, 2651, 2830
MbiI CCGCTC 1 cut(s) 409
MfeI CAATTG 1 cut(s) 482
MflI RGATCY 4 cut(s) 670, 1223, 1781, 2305
MhlI GDGCHC 6 cut(s) 8, 491, 844, 910, 1152, 2793
MlsI TGGCCA 2 cut(s) 350, 1059
MluNI TGGCCA 2 cut(s) 350, 1059
MlyI GAGTC 4 cut(s) 226, 590, 1891, 2827
MmeI TCCRAC 4 cut(s) 220, 277, 792, 2423
Mox20I TGGCCA 2 cut(s) 350, 1059
MroI TCCGGA 1 cut(s) 2693
MroXI GAANNNNTTC 1 cut(s) 2850
MscI TGGCCA 2 cut(s) 350, 1059
MseI TTAA 9 cut(s) 47, 852, 876, 1508, 1646, 1746, 2373, 2810, 2937
MslI CAYNNNNRTG 2 cut(s) 1508, 2520
Msp20I TGGCCA 2 cut(s) 350, 1059
MspI CCGG 7 cut(s) 236, 267, 426, 1466, 1980, 2694, 2755
MspR9I CCNGG 6 cut(s) 347, 422, 1146, 2043, 2343, 2755
MunI CAATTG 1 cut(s) 482
Mva1269I GAATGC 1 cut(s) 1871
MvaI CCWGG 5 cut(s) 347, 422, 1146, 2043, 2343
MvnI CGCG 2 cut(s) 435, 1160
MwoI GCNNNNNNNGC 5 cut(s) 220, 406, 422, 1040, 2585
NciI CCSGG 1 cut(s) 2755
NcoI CCATGG 1 cut(s) 1598
NdeI CATATG 3 cut(s) 1585, 1879, 2317
NheI GCTAGC 1 cut(s) 2062
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 5 cut(s) 318, 2459, 2591, 2651, 2830
NspI RCATGY 3 cut(s) 860, 2182, 2270
NspV TTCGAA 1 cut(s) 2858
PaeI GCATGC 2 cut(s) 860, 2182
PaqCI CACCTGC 1 cut(s) 1636
PceI AGGCCT 2 cut(s) 310, 473
PciSI GCTCTTC 1 cut(s) 1542
PcsI WCGNNNNNNNCGW 1 cut(s) 2505
PctI GAATGC 1 cut(s) 1871
PdmI GAANNNNTTC 1 cut(s) 2850
PfeI GAWTC 4 cut(s) 611, 716, 733, 1914
PflFI GACNNNGTC 1 cut(s) 758
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 2341
PkrI GCNGC 5 cut(s) 408, 437, 1276, 1336, 1471
PleI GAGTC 4 cut(s) 226, 590, 1891, 2827
PpsI GAGTC 4 cut(s) 226, 590, 1891, 2827
PshBI ATTAAT 2 cut(s) 1508, 2373
PsiI TTATAA 1 cut(s) 920
Psp1406I AACGTT 1 cut(s) 2499
Psp6I CCWGG 5 cut(s) 345, 420, 1144, 2041, 2341
PspEI GGTNACC 1 cut(s) 318
PspFI CCCAGC 3 cut(s) 225, 1867, 1906
PspGI CCWGG 5 cut(s) 345, 420, 1144, 2041, 2341
PspOMI GGGCCC 1 cut(s) 487
PspPI GGNCC 6 cut(s) 222, 418, 487, 488, 1138, 1861
PstI CTGCAG 1 cut(s) 1180
PstNI CAGNNNCTG 1 cut(s) 548
PsuI RGATCY 4 cut(s) 670, 1223, 1781, 2305
PsyI GACNNNGTC 1 cut(s) 758
RsaI GTAC 5 cut(s) 940, 1163, 1962, 2435, 2541
RsaNI GTAC 5 cut(s) 939, 1162, 1961, 2434, 2540
RseI CAYNNNNRTG 2 cut(s) 1508, 2520
SalI GTCGAC 1 cut(s) 2893
SapI GCTCTTC 1 cut(s) 1542
SaqAI TTAA 9 cut(s) 47, 852, 876, 1508, 1646, 1746, 2373, 2810, 2937
SatI GCNGC 5 cut(s) 407, 436, 1275, 1335, 1470
Sau96I GGNCC 6 cut(s) 222, 418, 487, 488, 1138, 1861
ScaI AGTACT 1 cut(s) 2541
SchI GAGTC 4 cut(s) 226, 590, 1891, 2827
ScrFI CCNGG 6 cut(s) 347, 422, 1146, 2043, 2343, 2755
SduI GDGCHC 6 cut(s) 8, 491, 844, 910, 1152, 2793
SfaNI GCATC 3 cut(s) 937, 1000, 1243
SfcI CTRYAG 1 cut(s) 1176
SfuI TTCGAA 1 cut(s) 2858
SinI GGWCC 2 cut(s) 418, 1861
SmiI ATTTAAAT 1 cut(s) 48
SmiMI CAYNNNNRTG 2 cut(s) 1508, 2520
SphI GCATGC 2 cut(s) 860, 2182
SseBI AGGCCT 2 cut(s) 310, 473
SspMI CTAG 5 cut(s) 521, 1604, 2063, 2310, 2726
StuI AGGCCT 2 cut(s) 310, 473
StyD4I CCNGG 6 cut(s) 345, 420, 1144, 2041, 2341, 2753
StyI CCWWGG 2 cut(s) 1598, 2870
SwaI ATTTAAAT 1 cut(s) 48
TaaI ACNGT 7 cut(s) 179, 362, 760, 2020, 2438, 2459, 2465
TaiI ACGT 4 cut(s) 468, 2502, 2682, 2900
TatI WGTACW 3 cut(s) 938, 2433, 2539
TauI GCSGC 2 cut(s) 409, 438
TfiI GAWTC 4 cut(s) 611, 716, 733, 1914
Tru1I TTAA 9 cut(s) 47, 852, 876, 1508, 1646, 1746, 2373, 2810, 2937
Tru9I TTAA 9 cut(s) 47, 852, 876, 1508, 1646, 1746, 2373, 2810, 2937
TseFI GTSAC 5 cut(s) 318, 2459, 2591, 2651, 2830
TseI GCWGC 3 cut(s) 1274, 1334, 1469
Tsp45I GTSAC 5 cut(s) 318, 2459, 2591, 2651, 2830
TspDTI ATGAA 9 cut(s) 279, 1202, 1391, 1467, 1540, 2126, 2391, 2510, 2538
TspGWI ACGGA 2 cut(s) 232, 921
Tth111I GACNNNGTC 1 cut(s) 758
Van91I CCANNNNNTGG 1 cut(s) 291
VpaK11BI GGWCC 2 cut(s) 418, 1861
VspI ATTAAT 2 cut(s) 1508, 2373
XagI CCTNNNNNAGG 1 cut(s) 644
XapI RAATTY 2 cut(s) 43, 1027
XceI RCATGY 3 cut(s) 860, 2182, 2270
XcmI CCANNNNNNNNNTGG 1 cut(s) 842
XmiI GTMKAC 3 cut(s) 1182, 1698, 2894
XmnI GAANNNNTTC 1 cut(s) 2850
XspI CTAG 5 cut(s) 521, 1604, 2063, 2310, 2726
ZraI GACGTC 1 cut(s) 2898
ZrmI AGTACT 1 cut(s) 2541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.