pycom08g02480

Cytosolic purine 5'-nucleotidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003145
Physical Location & Seq
Reverse (-)
1823905 .. 1827391
3487 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1629 bp
ATGGAAAAGGGCCACGAGACTGCTGTAGGAGGTGACAAGCCATCGCATTTTGCAAATATGGGCTCGACGGATGGTGCTCAAAAACCATGTGTGTGGTCTTCTCCCCAAGGAGGATGTAAAATAGACTTAAGAAAGCAGATTTTCTGCAACAGATCGTTGAATATGAAGAATATTGTCGCGGTAGGATTTGATATGGATTACACATTGGCACAGTACAAGCCGGAAACCTTTGAGTCTCTTGCATACGATGGAACTATCCGAAAGTTGGTGTATGATCTAGGATACCCTCGTGAGTTGCTGAATTGGTCCTTCGATTGGACATATATGGTCAGAGGGTTGGTTCTGGATAAGAAGAGAGGCAACATCTTGAAGATGGATCGACAAAAGTATGTGAAAGTTGCTTACCATGGATTCAAAGAGTTATCTAAGGAAGATAAAGTTAACACCTATGGAAATACATTAATACGGGATTCCTTTGACGAGCCAGATTATATTATCATTGATACCCTCTTTGCTCTAGCTGAAGCTTACATGTTTGCCCAACTGGTTGACTTCAAAGACAAAAATCCAGGAAAAATTCCAGAGGGTGTTGATTATGCTGTTATGTACAAGGATGTTCGAGCTGCTGTTGATTTGTGCCACCGTGATGGAACTTTAAAGCAAATGGTGGCAAAAGACCCTCAGCGGTATATTAATGAAGATACCTCCATAGTTCCGATGCTCAAAATGCTGAGAGATTCTGGCCGTGCTACATTCCTAGTGACAAACAGTTTATGGGACTACACAAACATTGTGATGAATTTCCTCTGCGAGTCGCGTACAGTGGATGGTGGTAGAAAATGCAATTTCGACTGGCTTAAATACTTTGATGTTGTCATCAGTGGCAGTGCAAAGCCAAGCTTCTTTCATGATGGTAACCGTGCTAATCTGTTTGAGGTAGAACTCGAGTCGGGGATGCTAATCAATACTGATAATGGTTCTCCTATGGCTCAGGTTGGAAGTTCTTCACCTGCACCTATGTTATCGTTGAAGGGACAGAAGAAGGCTTGTAGAGTTTTCCAGGGAGGCAGCGTTGGCCATCTTCATAAACTGCTTTCAATTGAGTCAAGTTCACAGGTTCTTTATGTTGGTGACCATATATATGGAGATATTTTGCGCAGCAAAAAGGTTCTTGGGTGGAGAACAATGCTTGTCGTTCCAGAGCTAGAGAGGGAGGTTGAACTCCTATGGGAATTGAGAGATACCCGCAAGCAACTTCGATTGTTGAGGAATGAACGTGATCTGATTGAAGACAAAATACATCATCTAAAGTGGTCTCTCAAATATGACGATTTCAGTACTGTGGAGAAGCAAAAATTATCTTCTGAATGCAATGCTTTGGAGTTTCAAAGAGATCAAGTGCGTTTCGCCCATCAACAGGCTCAACGTGAATGTCACCAAATGTTTCATAAGATTTGGGGGCAACTCATGAAGACTGGCTATCAGAACTCCCGCTTTGCCCATCAGGTCGAGAGATTTGCCTGCCTTTATACTAGCCAGGTTTCCAACTTGAGTCTCTACTCGCCCGACAAATACTACAGGCCGAGCGAAGACTACATGCCCCATGAATTCGATATTCTTCCCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

543

Amino Acids

62.54

Weight (kDa)

7.58

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 47 - 537 9.9e-176 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1018
Acc16I TGCGCA 1 cut(s) 1157
Acc36I ACCTGC 1 cut(s) 1018
AccII CGCG 2 cut(s) 179, 817
AciI CCGC 4 cut(s) 179, 685, 1246, 1492
AclWI GGATC 1 cut(s) 384
AcoI YGGCCR 2 cut(s) 742, 1075
AcsI RAATTY 3 cut(s) 576, 799, 1607
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 4 cut(s) 215, 608, 820, 1339
AfiI CCNNNNNNNGG 4 cut(s) 110, 265, 315, 1417
AflII CTTAAG 1 cut(s) 127
AflIII ACRYGT 1 cut(s) 531
AgsI TTSAA 9 cut(s) 160, 370, 415, 556, 1030, 1098, 1220, 1289, 1388
AjnI CCWGG 3 cut(s) 568, 1059, 1536
AluBI AGCT 5 cut(s) 521, 527, 623, 900, 1204
AluI AGCT 5 cut(s) 521, 527, 623, 900, 1204
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 4 cut(s) 11, 240, 1320, 1559
AlwI GGATC 1 cut(s) 384
Ama87I CYCGRG 1 cut(s) 944
AoxI GGCC 4 cut(s) 10, 742, 1075, 1580
ApeKI GCWGC 3 cut(s) 623, 1068, 1158
ApoI RAATTY 3 cut(s) 576, 799, 1607
AseI ATTAAT 2 cut(s) 461, 693
Asp700I GAANNNNTTC 1 cut(s) 1003
AspLEI GCGC 1 cut(s) 1158
AspS9I GGNCC 2 cut(s) 10, 306
AsuHPI GGTGA 4 cut(s) 44, 999, 1142, 1427
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 1 cut(s) 306
BaeI ACNNNNGTAYC 2 cut(s) 274, 307
BalI TGGCCA 1 cut(s) 1077
BanII GRGCYC 1 cut(s) 65
BarI GAAGNNNNNNTAC 2 cut(s) 1281, 1313
BauI CACGAG 2 cut(s) 14, 288
BbsI GAAGAC 4 cut(s) 90, 1296, 1478, 1596
Bbv12I GWGCWC 1 cut(s) 79
BbvCI CCTCAGC 1 cut(s) 681
BbvI GCAGC 3 cut(s) 610, 1080, 1170
BceAI ACGGC 1 cut(s) 729
BcgI CGANNNNNNTGC 2 cut(s) 1499, 1533
BciT130I CCWGG 3 cut(s) 570, 1061, 1538
BciVI GTATCC 1 cut(s) 275
BcoDI GTCTC 4 cut(s) 11, 240, 1320, 1559
BfaI CTAG 5 cut(s) 278, 518, 758, 1205, 1533
BfmI CTRYAG 2 cut(s) 24, 1576
BfrI CTTAAG 1 cut(s) 127
BfuAI ACCTGC 1 cut(s) 1018
BfuI GTATCC 1 cut(s) 275
BisI GCNGC 3 cut(s) 624, 1069, 1159
BlsI GCNGC 3 cut(s) 625, 1070, 1160
BmcAI AGTACT 1 cut(s) 1339
Bme1390I CCNGG 3 cut(s) 570, 1061, 1538
Bme18I GGWCC 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 944
BmgT120I GGNCC 2 cut(s) 10, 306
BmrFI CCNGG 3 cut(s) 570, 1061, 1538
BmsI GCATC 2 cut(s) 708, 945
BpiI GAAGAC 4 cut(s) 90, 1296, 1478, 1596
Bpu10I CCTNAGC 2 cut(s) 681, 990
BpuEI CTTGAG 1 cut(s) 1570
BsaBI GATNNNNATC 1 cut(s) 959
BsaI GGTCTC 1 cut(s) 1320
BsaJI CCNNGG 3 cut(s) 106, 406, 1060
BsaXI ACNNNNNCTCC 2 cut(s) 1056, 1086
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 265, 315, 1417
Bse1I ACTGG 3 cut(s) 549, 857, 1480
Bse3DI GCAATG 1 cut(s) 1378
Bse8I GATNNNNATC 1 cut(s) 959
BseBI CCWGG 3 cut(s) 570, 1061, 1538
BseDI CCNNGG 3 cut(s) 106, 406, 1060
BseGI GGATG 5 cut(s) 76, 119, 619, 832, 960
BseJI GATNNNNATC 1 cut(s) 959
BseLI CCNNNNNNNGG 4 cut(s) 110, 265, 315, 1417
BseMI GCAATG 1 cut(s) 1378
BseMII CTCAG 3 cut(s) 695, 722, 1004
BseNI ACTGG 3 cut(s) 549, 857, 1480
BseXI GCAGC 3 cut(s) 610, 1080, 1170
BsgI GTGCAG 1 cut(s) 996
Bsh1236I CGCG 2 cut(s) 179, 817
BshFI GGCC 4 cut(s) 12, 744, 1077, 1582
BsiHKAI GWGCWC 1 cut(s) 79
BsiHKCI CYCGRG 1 cut(s) 944
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 2 cut(s) 791, 1047
BslI CCNNNNNNNGG 4 cut(s) 110, 265, 315, 1417
BsmAI GTCTC 4 cut(s) 11, 240, 1320, 1559
BsmFI GGGAC 2 cut(s) 791, 1047
BsmI GAATGC 1 cut(s) 1373
BsnI GGCC 4 cut(s) 12, 744, 1077, 1582
Bso31I GGTCTC 1 cut(s) 1320
BsoBI CYCGRG 1 cut(s) 944
Bsp1286I GDGCHC 2 cut(s) 65, 79
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 5 cut(s) 152, 274, 376, 1279, 1393
Bsp19I CCATGG 1 cut(s) 406
BspACI CCGC 4 cut(s) 179, 685, 1246, 1492
BspANI GGCC 4 cut(s) 12, 744, 1077, 1582
BspCNI CTCAG 3 cut(s) 694, 723, 1003
BspFNI CGCG 2 cut(s) 179, 817
BspHI TCATGA 2 cut(s) 907, 1467
BspMI ACCTGC 1 cut(s) 1018
BspPI GGATC 1 cut(s) 384
BspTI CTTAAG 1 cut(s) 127
BspTNI GGTCTC 1 cut(s) 1320
BsrDI GCAATG 1 cut(s) 1378
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 3 cut(s) 549, 857, 1480
BssECI CCNNGG 3 cut(s) 106, 406, 1060
BssMI GATC 5 cut(s) 152, 274, 376, 1279, 1393
BssSI CACGAG 2 cut(s) 14, 288
BssT1I CCWWGG 2 cut(s) 106, 406
Bst2BI CACGAG 2 cut(s) 14, 288
Bst2UI CCWGG 3 cut(s) 570, 1061, 1538
Bst4CI ACNGT 6 cut(s) 213, 644, 770, 823, 920, 1342
Bst6I CTCTTC 1 cut(s) 347
BstAFI CTTAAG 1 cut(s) 127
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 2 cut(s) 1250, 1522
BstDEI CTNAG 4 cut(s) 426, 681, 731, 990
BstDSI CCRYGG 1 cut(s) 406
BstEII GGTNACC 2 cut(s) 914, 1130
BstF5I GGATG 5 cut(s) 76, 119, 619, 832, 960
BstFNI CGCG 2 cut(s) 179, 817
BstHHI GCGC 1 cut(s) 1158
BstKTI GATC 5 cut(s) 155, 277, 379, 1282, 1396
BstMAI GTCTC 4 cut(s) 11, 240, 1320, 1559
BstMBI GATC 5 cut(s) 152, 274, 376, 1279, 1393
BstMWI GCNNNNNNNGC 2 cut(s) 727, 1074
BstNI CCWGG 3 cut(s) 570, 1061, 1538
BstNSI RCATGY 2 cut(s) 535, 1600
BstPI GGTNACC 2 cut(s) 914, 1130
BstSCI CCNGG 3 cut(s) 568, 1059, 1536
BstSFI CTRYAG 2 cut(s) 24, 1576
BstUI CGCG 2 cut(s) 179, 817
BstV1I GCAGC 3 cut(s) 610, 1080, 1170
BstV2I GAAGAC 4 cut(s) 90, 1296, 1478, 1596
BstXI CCANNNNNNTGG 3 cut(s) 93, 647, 1142
BsuI GTATCC 1 cut(s) 275
BsuRI GGCC 4 cut(s) 12, 744, 1077, 1582
BtgI CCRYGG 1 cut(s) 406
BtgZI GCGATG 1 cut(s) 27
BtsCI GGATG 5 cut(s) 76, 119, 619, 832, 960
BtsI GCAGTG 1 cut(s) 892
BtsIMutI CAGTG 3 cut(s) 828, 886, 892
BveI ACCTGC 1 cut(s) 1018
Cac8I GCNNGC 2 cut(s) 1250, 1522
CciI TCATGA 2 cut(s) 907, 1467
CfoI GCGC 1 cut(s) 1158
Cfr13I GGNCC 2 cut(s) 10, 306
Csp6I GTAC 4 cut(s) 214, 607, 819, 1338
CviAII CATG 7 cut(s) 87, 407, 532, 908, 1468, 1597, 1604
CviQI GTAC 4 cut(s) 214, 607, 819, 1338
DdeI CTNAG 4 cut(s) 426, 681, 731, 990
DpnI GATC 5 cut(s) 154, 276, 378, 1281, 1395
DpnII GATC 5 cut(s) 152, 274, 376, 1279, 1393
DraI TTTAAA 1 cut(s) 657
EaeI YGGCCR 2 cut(s) 742, 1075
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 106, 406
Eco24I GRGCYC 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 1320
Eco47I GGWCC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 944
Eco91I GGTNACC 2 cut(s) 914, 1130
EcoO65I GGTNACC 2 cut(s) 914, 1130
EcoRI GAATTC 1 cut(s) 1607
EcoRII CCWGG 3 cut(s) 568, 1059, 1536
EcoT14I CCWWGG 2 cut(s) 106, 406
EcoT38I GRGCYC 1 cut(s) 65
ErhI CCWWGG 2 cut(s) 106, 406
FaeI CATG 7 cut(s) 90, 410, 535, 911, 1471, 1600, 1607
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FaqI GGGAC 2 cut(s) 791, 1047
FatI CATG 7 cut(s) 86, 406, 531, 907, 1467, 1596, 1603
FauI CCCGC 2 cut(s) 1253, 1499
Fnu4HI GCNGC 3 cut(s) 624, 1069, 1159
FokI GGATG 5 cut(s) 83, 126, 626, 839, 967
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 3 cut(s) 624, 1069, 1159
FspBI CTAG 5 cut(s) 278, 518, 758, 1205, 1533
FspI TGCGCA 1 cut(s) 1157
GlaI GCGC 1 cut(s) 1157
GluI GCNGC 3 cut(s) 624, 1069, 1159
HaeIII GGCC 4 cut(s) 12, 744, 1077, 1582
HapII CCGG 1 cut(s) 221
HhaI GCGC 1 cut(s) 1158
Hin1II CATG 7 cut(s) 90, 410, 535, 911, 1471, 1600, 1607
Hin6I GCGC 1 cut(s) 1156
HinP1I GCGC 1 cut(s) 1156
HincII GTYRAC 2 cut(s) 442, 550
HindII GTYRAC 2 cut(s) 442, 550
HindIII AAGCTT 2 cut(s) 525, 898
HinfI GANTC 8 cut(s) 233, 411, 470, 737, 812, 947, 1103, 1552
HpaI GTTAAC 1 cut(s) 442
HpaII CCGG 1 cut(s) 221
HphI GGTGA 4 cut(s) 44, 999, 1142, 1427
Hpy166II GTNNAC 3 cut(s) 442, 550, 1112
Hpy188I TCNGA 6 cut(s) 260, 332, 717, 1284, 1366, 1485
Hpy188III TCNNGA 8 cut(s) 290, 344, 367, 581, 908, 1199, 1468, 1510
Hpy8I GTNNAC 3 cut(s) 442, 550, 1112
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 3 cut(s) 319, 1024, 1036
HpyCH4III ACNGT 6 cut(s) 213, 644, 770, 823, 920, 1342
HpyCH4IV ACGT 2 cut(s) 1276, 1426
HpyCH4V TGCA 7 cut(s) 53, 147, 242, 843, 890, 1013, 1371
HpyF10VI GCNNNNNNNGC 2 cut(s) 727, 1074
HpyF3I CTNAG 4 cut(s) 426, 681, 731, 990
HpySE526I ACGT 2 cut(s) 1276, 1426
Hsp92II CATG 7 cut(s) 90, 410, 535, 911, 1471, 1600, 1607
HspAI GCGC 1 cut(s) 1156
KspAI GTTAAC 1 cut(s) 442
Kzo9I GATC 5 cut(s) 152, 274, 376, 1279, 1393
Lsp1109I GCAGC 3 cut(s) 610, 1080, 1170
LweI GCATC 2 cut(s) 708, 945
MaeI CTAG 5 cut(s) 278, 518, 758, 1205, 1533
MaeII ACGT 2 cut(s) 1276, 1426
MaeIII GTNAC 5 cut(s) 32, 760, 914, 1130, 1433
MalI GATC 5 cut(s) 154, 276, 378, 1281, 1395
MboI GATC 5 cut(s) 152, 274, 376, 1279, 1393
MfeI CAATTG 1 cut(s) 1098
MhlI GDGCHC 2 cut(s) 65, 79
MlsI TGGCCA 1 cut(s) 1077
MluCI AATT 8 cut(s) 301, 576, 799, 844, 1098, 1232, 1355, 1607
MluNI TGGCCA 1 cut(s) 1077
MlyI GAGTC 5 cut(s) 242, 821, 956, 1112, 1561
MmeI TCCRAC 2 cut(s) 976, 1569
Mox20I TGGCCA 1 cut(s) 1077
MroXI GAANNNNTTC 1 cut(s) 1003
MscI TGGCCA 1 cut(s) 1077
MseI TTAA 6 cut(s) 128, 441, 461, 656, 693, 858
MslI CAYNNNNRTG 4 cut(s) 91, 645, 794, 1140
Msp20I TGGCCA 1 cut(s) 1077
MspA1I CMGCKG 1 cut(s) 685
MspCI CTTAAG 1 cut(s) 127
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 3 cut(s) 570, 1061, 1538
MunI CAATTG 1 cut(s) 1098
Mva1269I GAATGC 1 cut(s) 1373
MvaI CCWGG 3 cut(s) 570, 1061, 1538
MvnI CGCG 2 cut(s) 179, 817
MwoI GCNNNNNNNGC 2 cut(s) 727, 1074
NcoI CCATGG 1 cut(s) 406
NdeII GATC 5 cut(s) 152, 274, 376, 1279, 1393
NlaIII CATG 7 cut(s) 90, 410, 535, 911, 1471, 1600, 1607
NmeAIII GCCGAG 1 cut(s) 1608
NmuCI GTSAC 4 cut(s) 32, 760, 1130, 1433
NsbI TGCGCA 1 cut(s) 1157
NspI RCATGY 2 cut(s) 535, 1600
PaeR7I CTCGAG 1 cut(s) 944
PagI TCATGA 2 cut(s) 907, 1467
PaqCI CACCTGC 1 cut(s) 1018
PciI ACATGT 1 cut(s) 531
PctI GAATGC 1 cut(s) 1373
PdmI GAANNNNTTC 1 cut(s) 1003
PfeI GAWTC 3 cut(s) 411, 470, 737
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 3 cut(s) 625, 1070, 1160
PleI GAGTC 5 cut(s) 241, 820, 955, 1111, 1560
PpsI GAGTC 5 cut(s) 241, 820, 955, 1111, 1560
PscI ACATGT 1 cut(s) 531
PshBI ATTAAT 2 cut(s) 461, 693
Psp6I CCWGG 3 cut(s) 568, 1059, 1536
PspEI GGTNACC 2 cut(s) 914, 1130
PspGI CCWGG 3 cut(s) 568, 1059, 1536
PspPI GGNCC 2 cut(s) 10, 306
PspXI VCTCGAGB 1 cut(s) 944
RsaI GTAC 4 cut(s) 215, 608, 820, 1339
RsaNI GTAC 4 cut(s) 214, 607, 819, 1338
RseI CAYNNNNRTG 4 cut(s) 91, 645, 794, 1140
SaqAI TTAA 6 cut(s) 128, 441, 461, 656, 693, 858
SatI GCNGC 3 cut(s) 624, 1069, 1159
Sau3AI GATC 5 cut(s) 152, 274, 376, 1279, 1393
Sau96I GGNCC 2 cut(s) 10, 306
ScaI AGTACT 1 cut(s) 1339
SchI GAGTC 5 cut(s) 242, 821, 956, 1112, 1561
ScrFI CCNGG 3 cut(s) 570, 1061, 1538
SduI GDGCHC 2 cut(s) 65, 79
SfaNI GCATC 2 cut(s) 708, 945
SfcI CTRYAG 2 cut(s) 24, 1576
Sfr274I CTCGAG 1 cut(s) 944
SinI GGWCC 1 cut(s) 306
SlaI CTCGAG 1 cut(s) 944
SmiMI CAYNNNNRTG 4 cut(s) 91, 645, 794, 1140
SmlI CTYRAG 3 cut(s) 127, 944, 1549
SmoI CTYRAG 3 cut(s) 127, 944, 1549
Sse9I AATT 8 cut(s) 301, 576, 799, 844, 1098, 1232, 1355, 1607
SsiI CCGC 4 cut(s) 179, 685, 1246, 1492
SspI AATATT 1 cut(s) 172
SspMI CTAG 5 cut(s) 278, 518, 758, 1205, 1533
StyD4I CCNGG 3 cut(s) 568, 1059, 1536
StyI CCWWGG 2 cut(s) 106, 406
TaaI ACNGT 6 cut(s) 213, 644, 770, 823, 920, 1342
TaiI ACGT 2 cut(s) 1279, 1429
TaqI TCGA 9 cut(s) 65, 312, 379, 619, 849, 945, 1258, 1509, 1611
TasI AATT 8 cut(s) 301, 576, 799, 844, 1098, 1232, 1355, 1607
TatI WGTACW 3 cut(s) 213, 606, 1337
TfiI GAWTC 3 cut(s) 411, 470, 737
Tru1I TTAA 6 cut(s) 128, 441, 461, 656, 693, 858
Tru9I TTAA 6 cut(s) 128, 441, 461, 656, 693, 858
TscAI CASTG 3 cut(s) 828, 886, 892
TseFI GTSAC 4 cut(s) 32, 760, 1130, 1433
TseI GCWGC 3 cut(s) 623, 1068, 1158
Tsp45I GTSAC 4 cut(s) 32, 760, 1130, 1433
TspDTI ATGAA 9 cut(s) 179, 711, 812, 896, 1073, 1287, 1436, 1484, 1620
TspGWI ACGGA 1 cut(s) 83
TspRI CASTG 3 cut(s) 828, 886, 892
Vha464I CTTAAG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 306
VspI ATTAAT 2 cut(s) 461, 693
XapI RAATTY 3 cut(s) 576, 799, 1607
XceI RCATGY 2 cut(s) 535, 1600
XhoI CTCGAG 1 cut(s) 944
XmnI GAANNNNTTC 1 cut(s) 1003
XspI CTAG 5 cut(s) 278, 518, 758, 1205, 1533
ZrmI AGTACT 1 cut(s) 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.