pycom08g05970
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0016560
Physical Location & Seq
Forward (+)
4533543 .. 4539916
6374 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2898 bp
ATGAGGTTTGATGGGAGGGCAGTTATGGCATTTCGGGTGGAGTTTGTGCTTCTGGTTCTTCTTCTTGGGTGTGTGAGCTTCGGTTATGGGGACTCACGGAACGATCAAGGAAGAACACTGCTGGGAATAAAGAAGGCATTTAGGGATGTGGACAATGTTCTCTATGAATGGACGGATTCACCCTCTTTAGATTACTGTGTTTGGAGAGGCGTCACATGTGATAACTCTCTCAATGTCATTGCACTTAATCTATCAGGTTTGAATCTTGGAGGGGAAATCTCATCTGTGATAGGAGATCTCGAAAGCCTGCAGTCTATGGGAAACCGCCTTTCCGGCCAGATCCCGGATGAGATTGGTGCCTGTGCAGCACTGCAAAACTTGGACTTGTCCTTCAATGAGATATTTGGAGACATACCATTTTCGATATCCAAGTTGAAACAGCTTGAAAATCTAGACCTAGCACAGAATAATCTCAGTGGGGAGATACCAAGGCTTATTTACTGGAATGAAGTTCTACAGTATCTTGGCTTGCGAGGGAACAATTTAGTTGGAACACTTTCTCCAGATATGTGCCAGTTAACTGGGTTATGGTATTTTGATGTGAGAAATAACAGCTTGACTGGTAGCATTCCTCAAAACATAGGGAACTGCACTGCCTTCCAGGTTTTGGATCTATCCTACAACCAGCTAACTGGAGGGATTCCGTTTAATATCGCGTTTTTGCAAGTTGCCACCTTGTCATTACAAGGTAATCAACTTTCTGGGCCTATCCCATCCGTGATTGGCCTGATGCAGGCTCTCGCCGTATTGGATTTGAGCAGCAACGCTTTAAGTGGACCAATCCCTCCTATCCTAGGGAATTTGACTTATACAGAGAAACTGTATTTGCATGCTAACAAGCTTAATGGATCAATCCCCCCAGAGCTTGGACAGATGACAAAGCTTCATTATTTGGAGTTGAATGATAACCATCTTACAGGACATATTCCACCGGAACTTGGGAAGCTGACTGATTTGTATGACCTGAACGTTGCAAACAACTATCTTCAAGGACCTATCCCTGATAATCTCAGCTCGTGTACAAATCTCAACAGCCTCAATGTGCATGGGAACAAGTTGAGTGGAACCATCCCGACTGCTCTTCAGAGGCTGGAGAGTATGACTTACTTAAATCTGTCCTCCAACTATCTTCGTGGTCCAATTCCTATCGAGCTGTCTTTTATTGGTAACTTGGATACCTTGGATATTTCAAATAACAAACTAAGTGGAACCATTCCTTCATCACTTGGGGATTTGGAACATCTTCTAAAGCTGAATTTGAGTCGAAACCATTTGACGGGATTTATTCCAGGGGAGTTTGGTAATTTAAGGAGTGTTATGGAAATAGACCTTTCAAATAATCAGCTCACAGGATTAATTCCTCAAGAGCTCAGTCAGCTACAGAACATGAATTTATTGAGATTAGAGCACAACAATATATCCGGGGATGTGGTATCGCTGATAAACTGCTTCAGCCTTTCTGCACTAAATGTATCTTACAACAACCTGGCAGGTGACGTTCCCACAAGCAAGAACTTCTCAAGGTTTTCACCAGACAGTTTTATTGGAAATCCTGATCTTTGTGGCCCTTGGCTCAATTCTCGATGTCATGAGTCTCGTCCAACAGAGCGAGCCACACTATCTAAACCTGCTATCCTGGGAATCGCTCTTGGGGCCCTTGTGATTCTTCTCATGATTCTTATTGCTGTATGCCGGCCATATAATCCAACTCCTTTTTCTGATGGTTCATTTGACAAACCCGTTAAGTACTCAAATCCGAAGCTGGTGATCCTTCACATGAATTTGGCACTTCATGTGTACGAGGACATCATGAGGATGACTGAAAACTTGAGCGAGAAGTATATAATTGGTTACGGTTCATCAAGTACAGTGTACAAATGTGTTCTGAAAAATTGTAAGCCAGTGGCCATCAAGAAACTCTACTCCCAGTATCATCGGTGCATCAAGGAATTTGAGACAGAACTTGCGACAGTAGGAAGCATCAAGCATCGGAATCTGGTGAGCCTCCAGGGGTACTCCTTGTCCTCCTCCGGAAACCTTCTCTTTTACGACTACATGGAAAATGGCAGTCTCTGGGATCACCTTCATGGCCCTTTCAAGAAGAAGAAGCTCGACTGGACAACTCGTCTCCAGATTGCCCTTGGAGCAGCCCAAGGGCTTGCCTATCTGCATCATGATTGCAGCCCCAGAATCATACACCGGGATATCAAGTCGTCCAATATTCTATTGGACAAGGATTTCGAAGCTCATCTAAATGATTTCGGCATTGCCAAGAACTTATGCCACTCAAAGACCCATACGTCTACTTACTTAATGGGCACGATTGGCTACATAGACCCTGAGTATGCGCGAACTTCCCGCCTCACTGAGAAGTCCGATGTGTATAGTTACGGAATTGTTCTGCTGGAGTTGCTGACAGGAAGGAAAGCCGTAGACAATGAATCCAATCTCCATCATTTGATATTATCTAAGACGGAGAGCAATGCTGTCATGGAAACCGTAGACCCAGAGATCTTGCCAACATGCACAGACCTCGGACAAGTGAAGAAGGTCTTCCAGCTTGCCCTTCTGTGCACAAAGCGGCAGCCAACAGACCGGCCAACAATGCACGAAGTAACTCGCGTGTTGGCCAGTCTCATGCCTTCCCCCGCACCACCAAAACAACCAACCCCTGCCAACCCGCCATCGTCACAGCTCCCGATCACCAAAGTGCCGTGCTACGTGGACGAGTACGCGAATGTGAAAACGCCCCACCTGCTAAATTGTCCATCAATGAGCACCTCAGATGCCCAGCTGTTTCTCAAGTTTGGAGAGGTAATTTCTCAGAACAGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009798 GO:0009888 GO:0009943 GO:0009944 GO:0009955 GO:0009965 GO:0009987 GO:0010016 GO:0010087 GO:0010103 GO:0010148 GO:0010229 GO:0010286 GO:0010374 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0022603 GO:0023052 GO:0030155 GO:0032501 GO:0032502 GO:0033218 GO:0033554 GO:0033612 GO:0034605 GO:0036211 GO:0038023 GO:0040008 GO:0042044 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048281 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051302 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0061458 GO:0065001 GO:0065007 GO:0070370 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090558 GO:0090567 GO:0090626 GO:0090698 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:1905421 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

966

Amino Acids

106.5

Weight (kDa)

5.99

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 74 3.2e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 246 - 371 1.3e-06 Leucine-rich repeat region
LRR_8 PF13855 339 - 397 2.3e-07 Leucine rich repeat
LRR_14 PF23598 357 - 492 7.4e-08 Leucine-rich repeat region
LRR_8 PF13855 410 - 469 6.7e-11 Leucine rich repeat
Pkinase PF00069 633 - 893 3.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 635 - 895 1.6e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016560)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26330
fragaria_vesca FvH4_2g30910 FvH4_2g30910 FvH4_2g30910
malus_domestica MD08G1071900.v1.1 MD15G1049300.v1.1
prunus_persica Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1 Prupe.1G403600_v2.0.a1
pyrus_communis pycom08g05970
rosa_chinensis RchiOBHm_Chr6g0311931
rosa_laevigata RLG00000010368
rosa_roxburghii Rroxscaffold_7G00157210
rosa_wichuraiana Rw6G044210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1541, 2823
AasI GACNNNNNNGTC 1 cut(s) 2359
Acc36I ACCTGC 3 cut(s) 1541, 1696, 2823
AccB1I GGYRCC 1 cut(s) 356
AccB7I CCANNNNNTGG 2 cut(s) 667, 926
AccI GTMKAC 3 cut(s) 2363, 2493, 2562
AccII CGCG 4 cut(s) 716, 2410, 2682, 2795
AccIII TCCGGA 1 cut(s) 2090
AciI CCGC 5 cut(s) 325, 2419, 2641, 2709, 2741
AclI AACGTT 1 cut(s) 1029
AclWI GGATC 5 cut(s) 334, 678, 916, 1822, 2145
AcoI YGGCCR 5 cut(s) 334, 1754, 1965, 2657, 2688
AcsI RAATTY 5 cut(s) 859, 1315, 1450, 1840, 2009
AcuI CTGAAG 2 cut(s) 1127, 1495
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 7 cut(s) 1081, 1808, 1859, 1927, 1934, 2075, 2792
AfiI CCNNNNNNNGG 7 cut(s) 343, 667, 793, 854, 926, 998, 1336
AflIII ACRYGT 1 cut(s) 215
AgsI TTSAA 9 cut(s) 262, 394, 436, 446, 961, 1049, 1251, 1395, 2158
AhdI GACNNNNNGTC 1 cut(s) 2184
AjnI CCWGG 5 cut(s) 660, 1348, 1545, 1695, 2067
AjuI GAANNNNNNNTTGG 2 cut(s) 1759, 1791
AleI CACNNNNGTG 1 cut(s) 2768
AloI GAACNNNNNNTCC 2 cut(s) 544, 576
Alw21I GWGCWC 4 cut(s) 1431, 1470, 2636, 2840
Alw26I GTCTC 6 cut(s) 402, 1659, 2009, 2135, 2192, 2699
Alw44I GTGCAC 1 cut(s) 2632
AlwI GGATC 5 cut(s) 334, 678, 916, 1822, 2145
AlwNI CAGNNNCTG 2 cut(s) 1150, 2133
Aor13HI TCCGGA 1 cut(s) 2090
ApaI GGGCCC 1 cut(s) 1717
ApaLI GTGCAC 1 cut(s) 2632
ApeKI GCWGC 5 cut(s) 365, 819, 2207, 2241, 2644
ApoI RAATTY 5 cut(s) 859, 1315, 1450, 1840, 2009
ArsI GACNNNNNNTTYG 2 cut(s) 2282, 2314
AseI ATTAAT 1 cut(s) 1415
Asp700I GAANNNNTTC 3 cut(s) 556, 1302, 2612
AspA2I CCTAGG 1 cut(s) 853
AspLEI GCGC 1 cut(s) 2410
AspS9I GGNCC 8 cut(s) 764, 836, 1052, 1196, 1625, 1713, 1714, 2150
AsuC2I CCSGG 3 cut(s) 344, 1483, 2261
AsuHPI GGTGA 7 cut(s) 171, 1565, 1581, 1837, 2071, 2132, 2755
AsuII TTCGAA 1 cut(s) 2301
AvaII GGWCC 3 cut(s) 836, 1052, 1196
AvrII CCTAGG 1 cut(s) 853
BaeGI GKGCMC 3 cut(s) 1717, 2381, 2636
BalI TGGCCA 2 cut(s) 1967, 2690
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 2 cut(s) 1431, 1717
BauI CACGAG 1 cut(s) 1075
BbsI GAAGAC 1 cut(s) 2605
Bbv12I GWGCWC 4 cut(s) 1431, 1470, 2636, 2840
BbvI GCAGC 5 cut(s) 377, 831, 2219, 2253, 2656
BccI CCATC 9 cut(s) 5, 781, 978, 1136, 1775, 1976, 2520, 2752, 2836
BceAI ACGGC 3 cut(s) 788, 2474, 2758
BcgI CGANNNNNNTGC 2 cut(s) 2575, 2609
BciT130I CCWGG 5 cut(s) 662, 1350, 1547, 1697, 2069
BciVI GTATCC 1 cut(s) 1228
BcnI CCSGG 3 cut(s) 344, 1483, 2261
BcoDI GTCTC 6 cut(s) 402, 1659, 2009, 2135, 2192, 2699
BfaI CTAG 3 cut(s) 452, 458, 854
BfmI CTRYAG 3 cut(s) 308, 515, 1439
BfuAI ACCTGC 3 cut(s) 1541, 1696, 2823
BfuI GTATCC 1 cut(s) 1228
BglI GCCNNNNNGGC 1 cut(s) 333
BglII AGATCT 2 cut(s) 295, 2571
BisI GCNGC 6 cut(s) 366, 820, 2208, 2242, 2642, 2645
BlnI CCTAGG 1 cut(s) 853
BlsI GCNGC 6 cut(s) 367, 821, 2209, 2243, 2643, 2646
BmcAI AGTACT 1 cut(s) 1808
Bme1390I CCNGG 8 cut(s) 344, 662, 1350, 1483, 1547, 1697, 2069, 2261
Bme18I GGWCC 3 cut(s) 836, 1052, 1196
BmeRI GACNNNNNGTC 1 cut(s) 2184
BmgT120I GGNCC 8 cut(s) 764, 836, 1052, 1196, 1625, 1713, 1714, 2150
BmiI GGNNCC 5 cut(s) 358, 1126, 1270, 1714, 1715
BmrFI CCNGG 8 cut(s) 344, 662, 1350, 1483, 1547, 1697, 2069, 2261
BmrI ACTGGG 2 cut(s) 591, 1981
BmsI GCATC 6 cut(s) 780, 2010, 2049, 2056, 2239, 2836
BmuI ACTGGG 2 cut(s) 591, 1981
BpiI GAAGAC 1 cut(s) 2605
BpmI CTGGAG 6 cut(s) 546, 714, 1172, 2051, 2174, 2486
Bpu14I TTCGAA 1 cut(s) 2301
BpuEI CTTGAG 4 cut(s) 1407, 1564, 1909, 2846
BpuMI CCSGG 3 cut(s) 344, 1483, 2261
BsaAI YACGTR 1 cut(s) 2782
BsaBI GATNNNNATC 1 cut(s) 969
BsaHI GRCGYC 1 cut(s) 210
BsaWI WCCGGW 2 cut(s) 991, 2090
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 7 cut(s) 343, 667, 793, 854, 926, 998, 1336
Bse118I RCCGGY 2 cut(s) 1752, 2655
Bse1I ACTGG 9 cut(s) 506, 574, 586, 625, 697, 1961, 1987, 2180, 2691
Bse3DI GCAATG 3 cut(s) 237, 2325, 2548
Bse8I GATNNNNATC 1 cut(s) 969
BseAI TCCGGA 1 cut(s) 2090
BseBI CCWGG 5 cut(s) 662, 1350, 1547, 1697, 2069
BseGI GGATG 6 cut(s) 151, 352, 773, 1128, 1492, 1881
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 7 cut(s) 343, 667, 793, 854, 926, 998, 1336
BseMI GCAATG 3 cut(s) 237, 2325, 2548
BseMII CTCAG 7 cut(s) 487, 1084, 1444, 2391, 2418, 2856, 2897
BseNI ACTGG 9 cut(s) 506, 574, 586, 625, 697, 1961, 1987, 2180, 2691
BseRI GAGGAG 1 cut(s) 2077
BseSI GKGCMC 3 cut(s) 1717, 2381, 2636
BseXI GCAGC 5 cut(s) 377, 831, 2219, 2253, 2656
BseYI CCCAGC 2 cut(s) 121, 2850
BsgI GTGCAG 3 cut(s) 384, 634, 1506
Bsh1236I CGCG 4 cut(s) 716, 2410, 2682, 2795
BshNI GGYRCC 1 cut(s) 356
BsiHKAI GWGCWC 4 cut(s) 1431, 1470, 2636, 2840
BsiSI CCGG 8 cut(s) 333, 344, 992, 1482, 1753, 2091, 2260, 2656
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 7 cut(s) 343, 667, 793, 854, 926, 998, 1336
BsmAI GTCTC 6 cut(s) 402, 1659, 2009, 2135, 2192, 2699
BsmBI CGTCTC 1 cut(s) 2192
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 1 cut(s) 627
Bsp119I TTCGAA 1 cut(s) 2301
Bsp120I GGGCCC 1 cut(s) 1713
Bsp1286I GDGCHC 6 cut(s) 1431, 1470, 1717, 2381, 2636, 2840
Bsp13I TCCGGA 1 cut(s) 2090
Bsp1407I TGTACA 2 cut(s) 1079, 1932
BspACI CCGC 5 cut(s) 325, 2419, 2641, 2709, 2741
BspCNI CTCAG 7 cut(s) 486, 1083, 1443, 2392, 2419, 2855, 2896
BspEI TCCGGA 1 cut(s) 2090
BspFNI CGCG 4 cut(s) 716, 2410, 2682, 2795
BspHI TCATGA 4 cut(s) 1648, 1731, 1869, 2233
BspLI GGNNCC 5 cut(s) 358, 1126, 1270, 1714, 1715
BspMAI CTGCAG 1 cut(s) 312
BspMI ACCTGC 3 cut(s) 1541, 1696, 2823
BspPI GGATC 5 cut(s) 334, 678, 916, 1822, 2145
BspQI GCTCTTC 1 cut(s) 1146
BspT104I TTCGAA 1 cut(s) 2301
BspT107I GGYRCC 1 cut(s) 356
BsrDI GCAATG 3 cut(s) 237, 2325, 2548
BsrFI RCCGGY 2 cut(s) 1752, 2655
BsrGI TGTACA 2 cut(s) 1079, 1932
BsrI ACTGG 9 cut(s) 506, 574, 586, 625, 697, 1961, 1987, 2180, 2691
BssAI RCCGGY 2 cut(s) 1752, 2655
BssNI GRCGYC 1 cut(s) 210
BssSI CACGAG 1 cut(s) 1075
BssT1I CCWWGG 6 cut(s) 488, 853, 1239, 1628, 2200, 2212
Bst2BI CACGAG 1 cut(s) 1075
Bst2UI CCWGG 5 cut(s) 662, 1350, 1547, 1697, 2069
Bst4CI ACNGT 9 cut(s) 197, 519, 882, 1598, 1916, 1930, 2032, 2560, 2891
Bst6I CTCTTC 1 cut(s) 1146
BstACI GRCGYC 1 cut(s) 210
BstAUI TGTACA 2 cut(s) 1079, 1932
BstBAI YACGTR 1 cut(s) 2782
BstBI TTCGAA 1 cut(s) 2301
BstC8I GCNNGC 8 cut(s) 308, 530, 795, 891, 1671, 1754, 2220, 2622
BstDEI CTNAG 9 cut(s) 473, 1070, 1262, 1430, 2400, 2427, 2529, 2842, 2883
BstENI CCTNNNNNAGG 2 cut(s) 791, 852
BstF5I GGATG 6 cut(s) 151, 352, 773, 1128, 1492, 1881
BstFNI CGCG 4 cut(s) 716, 2410, 2682, 2795
BstHHI GCGC 1 cut(s) 2410
BstMAI GTCTC 6 cut(s) 402, 1659, 2009, 2135, 2192, 2699
BstNI CCWGG 5 cut(s) 662, 1350, 1547, 1697, 2069
BstNSI RCATGY 3 cut(s) 219, 893, 2586
BstSCI CCNGG 8 cut(s) 342, 660, 1348, 1481, 1545, 1695, 2067, 2259
BstSFI CTRYAG 3 cut(s) 308, 515, 1439
BstSLI GKGCMC 3 cut(s) 1717, 2381, 2636
BstUI CGCG 4 cut(s) 716, 2410, 2682, 2795
BstV1I GCAGC 5 cut(s) 377, 831, 2219, 2253, 2656
BstV2I GAAGAC 1 cut(s) 2605
BstX2I RGATCY 4 cut(s) 295, 339, 670, 2571
BstXI CCANNNNNNTGG 2 cut(s) 581, 692
BstYI RGATCY 4 cut(s) 295, 339, 670, 2571
BsuI GTATCC 1 cut(s) 1228
BtsCI GGATG 6 cut(s) 151, 352, 773, 1128, 1492, 1881
BtsI GCAGTG 3 cut(s) 116, 368, 651
BtsIMutI CAGTG 8 cut(s) 116, 368, 481, 651, 1935, 1968, 2424, 2896
BveI ACCTGC 3 cut(s) 1541, 1696, 2823
Cac8I GCNNGC 8 cut(s) 308, 530, 795, 891, 1671, 1754, 2220, 2622
CaiI CAGNNNCTG 2 cut(s) 1150, 2133
CciI TCATGA 4 cut(s) 1648, 1731, 1869, 2233
CfoI GCGC 1 cut(s) 2410
Cfr10I RCCGGY 2 cut(s) 1752, 2655
Cfr13I GGNCC 8 cut(s) 764, 836, 1052, 1196, 1625, 1713, 1714, 2150
CseI GACGC 1 cut(s) 199
Csp6I GTAC 7 cut(s) 1080, 1807, 1858, 1926, 1933, 2074, 2791
CspCI CAANNNNNGTGG 4 cut(s) 1102, 1137, 1246, 1281
CviQI GTAC 7 cut(s) 1080, 1807, 1858, 1926, 1933, 2074, 2791
DdeI CTNAG 9 cut(s) 473, 1070, 1262, 1430, 2400, 2427, 2529, 2842, 2883
DrdI GACNNNNNNGTC 1 cut(s) 2359
DriI GACNNNNNGTC 1 cut(s) 2184
DseDI GACNNNNNNGTC 1 cut(s) 2359
EaeI YGGCCR 5 cut(s) 334, 1754, 1965, 2657, 2688
Eam1104I CTCTTC 1 cut(s) 1146
Eam1105I GACNNNNNGTC 1 cut(s) 2184
EarI CTCTTC 1 cut(s) 1146
Ecl136II GAGCTC 1 cut(s) 1429
Eco130I CCWWGG 6 cut(s) 488, 853, 1239, 1628, 2200, 2212
Eco24I GRGCYC 2 cut(s) 1431, 1717
Eco32I GATATC 2 cut(s) 426, 2266
Eco47I GGWCC 3 cut(s) 836, 1052, 1196
Eco53kI GAGCTC 1 cut(s) 1429
Eco57I CTGAAG 2 cut(s) 1127, 1495
EcoICRI GAGCTC 1 cut(s) 1429
EcoNI CCTNNNNNAGG 2 cut(s) 791, 852
EcoO109I RGGNCCY 3 cut(s) 1052, 1713, 1714
EcoRII CCWGG 5 cut(s) 660, 1348, 1545, 1695, 2067
EcoRV GATATC 2 cut(s) 426, 2266
EcoT14I CCWWGG 6 cut(s) 488, 853, 1239, 1628, 2200, 2212
EcoT38I GRGCYC 2 cut(s) 1431, 1717
ErhI CCWWGG 6 cut(s) 488, 853, 1239, 1628, 2200, 2212
Esp3I CGTCTC 1 cut(s) 2192
FalI AAGNNNNNCTT 2 cut(s) 2597, 2629
FaqI GGGAC 1 cut(s) 104
FauI CCCGC 3 cut(s) 2426, 2716, 2748
FblI GTMKAC 3 cut(s) 2363, 2493, 2562
Fnu4HI GCNGC 6 cut(s) 366, 820, 2208, 2242, 2642, 2645
FokI GGATG 6 cut(s) 158, 359, 760, 1115, 1499, 1888
FriOI GRGCYC 2 cut(s) 1431, 1717
Fsp4HI GCNGC 6 cut(s) 366, 820, 2208, 2242, 2642, 2645
FspBI CTAG 3 cut(s) 452, 458, 854
GlaI GCGC 1 cut(s) 2409
GluI GCNGC 6 cut(s) 366, 820, 2208, 2242, 2642, 2645
GsaI CCCAGC 2 cut(s) 125, 2854
GsuI CTGGAG 6 cut(s) 546, 714, 1172, 2051, 2174, 2486
HapII CCGG 8 cut(s) 333, 344, 992, 1482, 1753, 2091, 2260, 2656
HgaI GACGC 1 cut(s) 199
HhaI GCGC 1 cut(s) 2410
Hin1I GRCGYC 1 cut(s) 210
Hin6I GCGC 1 cut(s) 2408
HinP1I GCGC 1 cut(s) 2408
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 2 cut(s) 899, 941
HpaI GTTAAC 1 cut(s) 579
HpaII CCGG 8 cut(s) 333, 344, 992, 1482, 1753, 2091, 2260, 2656
HphI GGTGA 7 cut(s) 171, 1565, 1581, 1837, 2071, 2132, 2755
Hpy188I TCNGA 9 cut(s) 1146, 1780, 1818, 1947, 2052, 2437, 2597, 2845, 2886
HpyCH4III ACNGT 9 cut(s) 197, 519, 882, 1598, 1916, 1930, 2032, 2560, 2891
HpyCH4IV ACGT 4 cut(s) 1029, 1557, 2360, 2781
HpyF3I CTNAG 9 cut(s) 473, 1070, 1262, 1430, 2400, 2427, 2529, 2842, 2883
HpySE526I ACGT 4 cut(s) 1029, 1557, 2360, 2781
Hsp92I GRCGYC 1 cut(s) 210
HspAI GCGC 1 cut(s) 2408
Kpn2I TCCGGA 1 cut(s) 2090
KroI GCCGGC 1 cut(s) 1752
KroNI GCCGGC 1 cut(s) 1754
KspAI GTTAAC 1 cut(s) 579
LguI GCTCTTC 1 cut(s) 1146
LmnI GCTCC 2 cut(s) 2204, 2760
Lsp1109I GCAGC 5 cut(s) 377, 831, 2219, 2253, 2656
LweI GCATC 6 cut(s) 780, 2010, 2049, 2056, 2239, 2836
MaeI CTAG 3 cut(s) 452, 458, 854
MaeII ACGT 4 cut(s) 1029, 1557, 2360, 2781
MaeIII GTNAC 7 cut(s) 211, 1226, 1553, 1910, 2447, 2674, 2748
MflI RGATCY 4 cut(s) 295, 339, 670, 2571
MhlI GDGCHC 6 cut(s) 1431, 1470, 1717, 2381, 2636, 2840
MlsI TGGCCA 2 cut(s) 1967, 2690
MluNI TGGCCA 2 cut(s) 1967, 2690
MlyI GAGTC 3 cut(s) 86, 1330, 1661
MmeI TCCRAC 4 cut(s) 529, 1206, 1685, 1790
Mox20I TGGCCA 2 cut(s) 1967, 2690
MroI TCCGGA 1 cut(s) 2090
MroNI GCCGGC 1 cut(s) 1752
MroXI GAANNNNTTC 3 cut(s) 556, 1302, 2612
MscI TGGCCA 2 cut(s) 1967, 2690
MslI CAYNNNNRTG 4 cut(s) 1874, 2145, 2313, 2768
Msp20I TGGCCA 2 cut(s) 1967, 2690
MspA1I CMGCKG 1 cut(s) 2854
MspI CCGG 8 cut(s) 333, 344, 992, 1482, 1753, 2091, 2260, 2656
MspR9I CCNGG 8 cut(s) 344, 662, 1350, 1483, 1547, 1697, 2069, 2261
Mva1269I GAATGC 1 cut(s) 627
MvaI CCWGG 5 cut(s) 662, 1350, 1547, 1697, 2069
MvnI CGCG 4 cut(s) 716, 2410, 2682, 2795
NaeI GCCGGC 1 cut(s) 1754
NciI CCSGG 3 cut(s) 344, 1483, 2261
NgoMIV GCCGGC 1 cut(s) 1752
NlaIV GGNNCC 5 cut(s) 358, 1126, 1270, 1714, 1715
NmuCI GTSAC 3 cut(s) 211, 1553, 2748
NspI RCATGY 3 cut(s) 219, 893, 2586
NspV TTCGAA 1 cut(s) 2301
OliI CACNNNNGTG 1 cut(s) 2768
PaeI GCATGC 1 cut(s) 893
PagI TCATGA 4 cut(s) 1648, 1731, 1869, 2233
PaqCI CACCTGC 2 cut(s) 1541, 2823
PciI ACATGT 1 cut(s) 215
PciSI GCTCTTC 1 cut(s) 1146
PctI GAATGC 1 cut(s) 627
PdiI GCCGGC 1 cut(s) 1754
PdmI GAANNNNTTC 3 cut(s) 556, 1302, 2612
PfeI GAWTC 9 cut(s) 176, 262, 700, 1701, 1723, 1735, 2053, 2250, 2501
PflMI CCANNNNNTGG 2 cut(s) 667, 926
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 6 cut(s) 367, 821, 2209, 2243, 2643, 2646
PleI GAGTC 3 cut(s) 86, 1329, 1660
PpsI GAGTC 3 cut(s) 86, 1329, 1660
Ppu21I YACGTR 1 cut(s) 2782
PpuMI RGGWCCY 1 cut(s) 1052
PscI ACATGT 1 cut(s) 215
PshBI ATTAAT 1 cut(s) 1415
Psp124BI GAGCTC 1 cut(s) 1431
Psp1406I AACGTT 1 cut(s) 1029
Psp5II RGGWCCY 1 cut(s) 1052
Psp6I CCWGG 5 cut(s) 660, 1348, 1545, 1695, 2067
PspFI CCCAGC 2 cut(s) 121, 2850
PspGI CCWGG 5 cut(s) 660, 1348, 1545, 1695, 2067
PspN4I GGNNCC 5 cut(s) 358, 1126, 1270, 1714, 1715
PspOMI GGGCCC 1 cut(s) 1713
PspPI GGNCC 8 cut(s) 764, 836, 1052, 1196, 1625, 1713, 1714, 2150
PspPPI RGGWCCY 1 cut(s) 1052
PstI CTGCAG 1 cut(s) 312
PstNI CAGNNNCTG 2 cut(s) 1150, 2133
PsuI RGATCY 4 cut(s) 295, 339, 670, 2571
PvuII CAGCTG 1 cut(s) 2854
RsaI GTAC 7 cut(s) 1081, 1808, 1859, 1927, 1934, 2075, 2792
RsaNI GTAC 7 cut(s) 1080, 1807, 1858, 1926, 1933, 2074, 2791
RseI CAYNNNNRTG 4 cut(s) 1874, 2145, 2313, 2768
SacI GAGCTC 1 cut(s) 1431
SapI GCTCTTC 1 cut(s) 1146
SatI GCNGC 6 cut(s) 366, 820, 2208, 2242, 2642, 2645
Sau96I GGNCC 8 cut(s) 764, 836, 1052, 1196, 1625, 1713, 1714, 2150
ScaI AGTACT 1 cut(s) 1808
SchI GAGTC 3 cut(s) 86, 1330, 1661
ScrFI CCNGG 8 cut(s) 344, 662, 1350, 1483, 1547, 1697, 2069, 2261
SduI GDGCHC 6 cut(s) 1431, 1470, 1717, 2381, 2636, 2840
SfaNI GCATC 6 cut(s) 780, 2010, 2049, 2056, 2239, 2836
SfcI CTRYAG 3 cut(s) 308, 515, 1439
SfuI TTCGAA 1 cut(s) 2301
SinI GGWCC 3 cut(s) 836, 1052, 1196
SmiMI CAYNNNNRTG 4 cut(s) 1874, 2145, 2313, 2768
SmlI CTYRAG 4 cut(s) 1422, 1579, 1888, 2861
SmoI CTYRAG 4 cut(s) 1422, 1579, 1888, 2861
SphI GCATGC 1 cut(s) 893
SsiI CCGC 5 cut(s) 325, 2419, 2641, 2709, 2741
SspI AATATT 1 cut(s) 2281
SspMI CTAG 3 cut(s) 452, 458, 854
SstI GAGCTC 1 cut(s) 1431
StyD4I CCNGG 8 cut(s) 342, 660, 1348, 1481, 1545, 1695, 2067, 2259
StyI CCWWGG 6 cut(s) 488, 853, 1239, 1628, 2200, 2212
TaaI ACNGT 9 cut(s) 197, 519, 882, 1598, 1916, 1930, 2032, 2560, 2891
TaiI ACGT 4 cut(s) 1032, 1560, 2363, 2784
TaqI TCGA 7 cut(s) 300, 422, 1209, 1324, 1642, 2172, 2301
TatI WGTACW 4 cut(s) 1079, 1806, 1925, 1932
TauI GCSGC 1 cut(s) 2644
TfiI GAWTC 9 cut(s) 176, 262, 700, 1701, 1723, 1735, 2053, 2250, 2501
TscAI CASTG 8 cut(s) 123, 375, 481, 658, 1935, 1968, 2431, 2896
TseFI GTSAC 3 cut(s) 211, 1553, 2748
TseI GCWGC 5 cut(s) 365, 819, 2207, 2241, 2644
Tsp45I GTSAC 3 cut(s) 211, 1553, 2748
TspGWI ACGGA 6 cut(s) 112, 188, 693, 766, 2466, 2549
TspRI CASTG 8 cut(s) 123, 375, 481, 658, 1935, 1968, 2431, 2896
Van91I CCANNNNNTGG 2 cut(s) 667, 926
VneI GTGCAC 1 cut(s) 2632
VpaK11BI GGWCC 3 cut(s) 836, 1052, 1196
VspI ATTAAT 1 cut(s) 1415
XagI CCTNNNNNAGG 2 cut(s) 791, 852
XapI RAATTY 5 cut(s) 859, 1315, 1450, 1840, 2009
XbaI TCTAGA 1 cut(s) 451
XceI RCATGY 3 cut(s) 219, 893, 2586
XcmI CCANNNNNNNNNTGG 2 cut(s) 2198, 2284
XmaJI CCTAGG 1 cut(s) 853
XmiI GTMKAC 3 cut(s) 2363, 2493, 2562
XmnI GAANNNNTTC 3 cut(s) 556, 1302, 2612
XspI CTAG 3 cut(s) 452, 458, 854
ZrmI AGTACT 1 cut(s) 1808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.