pycom08g06540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0016274
Physical Location & Seq
Reverse (-)
5099638 .. 5099961
324 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06540.1

Sequence Viewer

Length: 324 bp
ATGGCCACCACATGCTCCAGCTTCTTCAGTTTCCGGTCAAGTAGTTCTTCCAGCGAGCCCAAGGTCCGGTCATCGTCGAGCCAAGGGTCATCACACGGGTGTGGCAAGGTTGATGGGGTGGGTATGTGGTTCATTCACAGTGTCTGTTCTGCTTTCTTCGCATCCTTAGAGAGGTGCTCTTGCATCCGTATCGTCACTCAAGAAGACGGCGATGACTGTAACGACTTGCCGTTGATTTTTAACGACGGGAATTTCCGGCATGATGTTGCTGATCACTCCACCACCAGGAGAAGAACAGGCAAAGGAAAGAAGTGTACGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

108

Amino Acids

11.7

Weight (kDa)

8.21

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016274)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32740
malus_domestica MD15G1066100.v1.1
prunus_persica Prupe.1G419900_v2.0.a1
pyrus_communis pycom08g06540 pycom15g06240
rosa_chinensis RchiOBHm_Chr6g0309931
rosa_laevigata RLG00000010523
rosa_multiflora Rmu_co8261055.1_g000001
rosa_roxburghii Rroxscaffold_7G00159030
rosa_rugosa Rorug06G0376000
rosa_samantha Rh6AG489900 Rh6BG499300 Rh6CG504200 Rh6DG490400
rosa_wichuraiana Rw6G042640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 250
AcuI CTGAAG 1 cut(s) 10
AfaI GTAC 2 cut(s) 316, 320
AfiI CCNNNNNNNGG 3 cut(s) 66, 171, 285
AjnI CCWGG 1 cut(s) 284
AleI CACNNNNGTG 2 cut(s) 97, 99
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 179
AlwNI CAGNNNCTG 1 cut(s) 144
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 64
AvaII GGWCC 1 cut(s) 64
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 60
BarI GAAGNNNNNNTAC 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 179
BccI CCATC 1 cut(s) 107
BceAI ACGGC 2 cut(s) 214, 223
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 1 cut(s) 286
BclI TGATCA 1 cut(s) 271
BfaI CTAG 1 cut(s) 322
Bme1390I CCNGG 1 cut(s) 286
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 2 cut(s) 170, 192
BpiI GAAGAC 1 cut(s) 210
BplI GAGNNNNNCTC 2 cut(s) 161, 193
BpuEI CTTGAG 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 318
BsaJI CCNNGG 2 cut(s) 60, 82
BsaWI WCCGGW 2 cut(s) 33, 66
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 171, 285
BseBI CCWGG 1 cut(s) 286
BseDI CCNNGG 2 cut(s) 60, 82
BseGI GGATG 2 cut(s) 161, 183
BseLI CCNNNNNNNGG 3 cut(s) 66, 171, 285
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 179
BsiSI CCGG 3 cut(s) 34, 67, 256
BslI CCNNNNNNNGG 3 cut(s) 66, 171, 285
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 60, 179
Bsp143I GATC 1 cut(s) 271
BspANI GGCC 1 cut(s) 5
BssECI CCNNGG 2 cut(s) 60, 82
BssMI GATC 1 cut(s) 271
BssT1I CCWWGG 2 cut(s) 60, 82
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 2 cut(s) 140, 218
BstBAI YACGTR 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 1 cut(s) 166
BstENI CCTNNNNNAGG 1 cut(s) 169
BstF5I GGATG 2 cut(s) 161, 183
BstKTI GATC 1 cut(s) 274
BstMBI GATC 1 cut(s) 271
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNI CCWGG 1 cut(s) 286
BstNSI RCATGY 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 284
BstSNI TACGTA 1 cut(s) 318
BstV2I GAAGAC 1 cut(s) 210
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 225
BtsCI GGATG 2 cut(s) 161, 183
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 56
CaiI CAGNNNCTG 1 cut(s) 144
Cfr13I GGNCC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 315, 319
CviAII CATG 2 cut(s) 12, 260
CviJI RGCY 4 cut(s) 5, 21, 58, 81
CviKI_1 RGCY 4 cut(s) 5, 21, 58, 81
CviQI GTAC 2 cut(s) 315, 319
DdeI CTNAG 1 cut(s) 166
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 3
Eco105I TACGTA 1 cut(s) 318
Eco130I CCWWGG 2 cut(s) 60, 82
Eco24I GRGCYC 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 284
EcoT14I CCWWGG 2 cut(s) 60, 82
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 60, 82
FaeI CATG 2 cut(s) 15, 263
FaiI YATR 3 cut(s) 13, 125, 261
FalI AAGNNNNNCTT 2 cut(s) 31, 63
FatI CATG 2 cut(s) 11, 259
FbaI TGATCA 1 cut(s) 271
FokI GGATG 2 cut(s) 148, 170
FriOI GRGCYC 1 cut(s) 60
FspBI CTAG 1 cut(s) 322
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 34, 67, 256
Hin1II CATG 2 cut(s) 15, 263
HpaII CCGG 3 cut(s) 34, 67, 256
Hpy166II GTNNAC 1 cut(s) 315
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 1 cut(s) 315
Hpy99I CGWCG 2 cut(s) 79, 248
HpyCH4III ACNGT 2 cut(s) 140, 218
HpyCH4IV ACGT 1 cut(s) 317
HpyCH4V TGCA 1 cut(s) 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 1 cut(s) 166
HpySE526I ACGT 1 cut(s) 317
Hsp92II CATG 2 cut(s) 15, 263
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 1 cut(s) 20
LpnPI CCDG 8 cut(s) 31, 47, 64, 80, 269, 271, 282, 298
LweI GCATC 2 cut(s) 170, 192
MaeI CTAG 1 cut(s) 322
MaeII ACGT 1 cut(s) 317
MaeIII GTNAC 2 cut(s) 193, 218
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 5 cut(s) 16, 39, 148, 215, 303
MhlI GDGCHC 2 cut(s) 60, 179
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 250
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 1 cut(s) 165
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 240
MslI CAYNNNNRTG 2 cut(s) 97, 99
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 34, 67, 256
MspR9I CCNGG 1 cut(s) 286
MvaI CCWGG 1 cut(s) 286
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeII GATC 1 cut(s) 271
NlaIII CATG 2 cut(s) 15, 263
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 1 cut(s) 15
OliI CACNNNNGTG 2 cut(s) 97, 99
Ppu21I YACGTR 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 284
PspGI CCWGG 1 cut(s) 284
PspPI GGNCC 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 144
RsaI GTAC 2 cut(s) 316, 320
RsaNI GTAC 2 cut(s) 315, 319
RseI CAYNNNNRTG 2 cut(s) 97, 99
SaqAI TTAA 1 cut(s) 240
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 286
SduI GDGCHC 2 cut(s) 60, 179
SetI ASST 5 cut(s) 23, 66, 111, 176, 320
SfaNI GCATC 2 cut(s) 170, 192
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 2 cut(s) 97, 99
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SnaBI TACGTA 1 cut(s) 318
Sse9I AATT 1 cut(s) 250
SspMI CTAG 1 cut(s) 322
StyD4I CCNGG 1 cut(s) 284
StyI CCWWGG 2 cut(s) 60, 82
TaaI ACNGT 2 cut(s) 140, 218
TaiI ACGT 1 cut(s) 320
TaqI TCGA 1 cut(s) 77
TasI AATT 1 cut(s) 250
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 193
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 1 cut(s) 121
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 64
XagI CCTNNNNNAGG 1 cut(s) 169
XapI RAATTY 1 cut(s) 250
XceI RCATGY 1 cut(s) 15
XspI CTAG 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.