Rorug06G0376000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016274
Physical Location & Seq
Reverse (-)
52273147 .. 52277421
4275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0376000.1

Sequence Viewer

Length: 1710 bp
ATGGACGAATTCGGTGTTTTGACGGAGAGGTTTGGCCTGAAACGGCAAGGCAAATCGGCGCCGATGGCCGCATCCAGAAGAGCAACACCCACCAACAACCCCCAAACGACGTCGTATGCATCCTCCTCAAGATCTTCCGCTAATTCCAATTCCAATTCCAATTCCGCCTACGCTTCTTTTCTCGACGATCAAACCGGCTTCTTCTCCCAATCGAGCTCTACCCAAAACACGGCCGGCTCCGGCGGATTCAACGATTACGTCGGCATCTTCGGCGGCGGAGTCACTAGACCCGCACATCAGCAATCCCAAGGCTCCGACTTTAATTTGGATTCCATGTTTTCGGGTTCGGGTGCCAATACGTCGTCGTTTGACGACGGCTTTGATGTGTTTGGGAGCTCCGGTGGTTCGGTTAATAGTAATGTGGAGGAAGATGACTTTTTCGGGTCGTTTTCTTCGGCTTCGAAACAGAGTGCAGCTCCGAGCAATGACCTGCTGGGTGATCTCGGTGGAGTAGCAAGGAAATTGCAGAGTACGAGCAATAGTAATAGAAATGGGAATACGACCGGAACGCCGGCGAACAATGCAGCTGATTCTGATGATTTGTTACCTGGCTTTGGTGTTAGCAATGCTTCCAATAGTGGAACTTACACAGACGAAAGTCGTACTCAGCAATCAACTGTTAATTCAACTAAGTCAAACTTCAGTTCACCAATGGATGACCCTTTTGTGGTCTTAGAATCAGTTTCAAGTTCGACACCAGCAGATTCCTTAGATTATCTTTCAGAACTGGAACAATTCAGTAAGATGAGTAATTCTGGAGGCGCGAACCAGGGTGGTTCATCAAATAGTTCTACAAAACTGAAAGCGCCCCCAAAACCAGCTCAAGTTTCAAAGGAAAGGAGCTCTGGTGTGTCTTCTATAGATGATCTTGAGAAATTTGCCAGTGCTGGGGTGCCAAATAGTGCTCCCAGAGTTCCTTCTCGTAACGAAGGGGTAGAATCCTCAGCCACTAGACATGGTAAATCAAGTGGAGATGATCTAGATTTCATGTTGAGCACAGGTTTTCGATCTAGCAGTGTACCGAAGTCAAGGGTTAAAAATGCGGACCCAATATTTGATGCACAGACGGACAACAGAGGAGGGCCTAGGCCCCAAAGGACATCTTCTAGGACCTCAGGTAGCATGAAGAAGTCCCCTTCTGCAAGTGGTATCTTTGATGACCTCTTTTCAATGGATGGAGCTTCCCCAATGGTTGCAGAATTTGAGGAAGTTGACGGTGAAAGTGAAGAAAGACGAAAAGCCAGATTGGGACGGCACCAGAGAACAAAGGAACGCGCGTTGCAAGCAGTGGCAGATATGAACCAACGCGATTTGCGTACTCAGCAGGAGCAGGAAGAGAGGCGTAGAATTGCTGAGGCTATGGATGCTAAAATAAAGGGATGGGCTGCAGGGAAAGAAGGGAATATGCGTGCACTATTGTCTTCATTGCAATCCGTTCTTTGGCCTGACTGTGGATGGGAGCCAGTTTCACTCACAGATTTGATTACTTCTGGTTCAGTTAAAAAGGTTTACAGAAAGGCCACATTGTGTGTCCATCCAGATAAGGTTCAACAGAAAGGAGCTAGTCTTCAACAAAAATATACTGCAGAGAAGGTTTTTGATATTCTTAAGGAAGCTTGGACCAAGTTCAACAAGGAGGAACTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

569

Amino Acids

60.67

Weight (kDa)

5.58

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016274)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32740
malus_domestica MD15G1066100.v1.1
prunus_persica Prupe.1G419900_v2.0.a1
pyrus_communis pycom08g06540 pycom15g06240
rosa_chinensis RchiOBHm_Chr6g0309931
rosa_laevigata RLG00000010523
rosa_multiflora Rmu_co8261055.1_g000001
rosa_roxburghii Rroxscaffold_7G00159030
rosa_rugosa Rorug06G0376000
rosa_samantha Rh6AG489900 Rh6BG499300 Rh6CG504200 Rh6DG490400
rosa_wichuraiana Rw6G042640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 113
Acc36I ACCTGC 1 cut(s) 498
AccB1I GGYRCC 4 cut(s) 58, 350, 952, 1314
AccII CGCG 4 cut(s) 824, 1335, 1337, 1368
AciI CCGC 8 cut(s) 69, 138, 165, 243, 273, 276, 291, 1103
AcoI YGGCCR 2 cut(s) 66, 231
AcsI RAATTY 3 cut(s) 8, 935, 1259
AcuI CTGAAG 1 cut(s) 685
AcyI GRCGYC 2 cut(s) 59, 110
AdeI CACNNNGTG 1 cut(s) 1587
AfaI GTAC 4 cut(s) 532, 664, 1080, 1378
AfiI CCNNNNNNNGG 6 cut(s) 229, 614, 727, 948, 1500, 1511
AflII CTTAAG 1 cut(s) 1667
AgsI TTSAA 8 cut(s) 250, 687, 747, 891, 1230, 1610, 1631, 1690
AjnI CCWGG 2 cut(s) 607, 828
AluBI AGCT 9 cut(s) 216, 396, 476, 587, 881, 903, 1241, 1622, 1676
AluI AGCT 9 cut(s) 216, 396, 476, 587, 881, 903, 1241, 1622, 1676
Alw21I GWGCWC 6 cut(s) 218, 398, 905, 967, 1058, 1474
Alw44I GTGCAC 1 cut(s) 1470
AoxI GGCC 7 cut(s) 34, 66, 231, 1142, 1148, 1502, 1578
ApaLI GTGCAC 1 cut(s) 1470
ApeKI GCWGC 3 cut(s) 473, 584, 1445
ApoI RAATTY 3 cut(s) 8, 935, 1259
ArsI GACNNNNNNTTYG 4 cut(s) 362, 394, 1097, 1129
AspA2I CCTAGG 1 cut(s) 1145
AspLEI GCGC 4 cut(s) 61, 824, 868, 1337
AspS9I GGNCC 5 cut(s) 1105, 1142, 1149, 1170, 1680
AsuHPI GGTGA 3 cut(s) 509, 699, 1289
AsuII TTCGAA 1 cut(s) 461
AvaII GGWCC 3 cut(s) 1105, 1170, 1680
AvrII CCTAGG 1 cut(s) 1145
AxyI CCTNAGG 1 cut(s) 1174
BaeGI GKGCMC 1 cut(s) 1474
BanI GGYRCC 4 cut(s) 58, 350, 952, 1314
BanII GRGCYC 3 cut(s) 218, 398, 905
BbsI GAAGAC 3 cut(s) 906, 1473, 1619
Bbv12I GWGCWC 6 cut(s) 218, 398, 905, 967, 1058, 1474
BbvCI CCTCAGC 2 cut(s) 1003, 1413
BbvI GCAGC 3 cut(s) 485, 596, 1432
BccI CCATC 5 cut(s) 58, 1229, 1434, 1509, 1602
BceAI ACGGC 4 cut(s) 59, 246, 391, 1328
BciT130I CCWGG 2 cut(s) 609, 830
BfaI CTAG 7 cut(s) 285, 1011, 1040, 1071, 1146, 1167, 1623
BfmI CTRYAG 3 cut(s) 918, 1446, 1644
BfoI RGCGCY 2 cut(s) 62, 869
BfrI CTTAAG 1 cut(s) 1667
BfuAI ACCTGC 1 cut(s) 498
BglII AGATCT 1 cut(s) 131
BisI GCNGC 5 cut(s) 69, 274, 474, 585, 1446
BlnI CCTAGG 1 cut(s) 1145
BlsI GCNGC 5 cut(s) 70, 275, 475, 586, 1447
Bme1390I CCNGG 2 cut(s) 609, 830
Bme18I GGWCC 3 cut(s) 1105, 1170, 1680
BmgT120I GGNCC 5 cut(s) 1105, 1142, 1149, 1170, 1680
BmiI GGNNCC 9 cut(s) 60, 238, 313, 352, 954, 1107, 1151, 1316, 1521
BmrFI CCNGG 2 cut(s) 609, 830
BmsI GCATC 5 cut(s) 80, 128, 273, 1108, 1414
BoxI GACNNNNGTC 1 cut(s) 657
BpiI GAAGAC 3 cut(s) 906, 1473, 1619
BplI GAGNNNNNCTC 2 cut(s) 460, 492
BpmI CTGGAG 1 cut(s) 837
Bpu10I CCTNAGC 2 cut(s) 1003, 1413
Bpu14I TTCGAA 1 cut(s) 461
BpuEI CTTGAG 3 cut(s) 112, 867, 950
BsaHI GRCGYC 2 cut(s) 59, 110
BsaJI CCNNGG 3 cut(s) 307, 829, 1145
BsaWI WCCGGW 2 cut(s) 398, 563
BsaXI ACNNNNNCTCC 4 cut(s) 385, 415, 892, 922
Bsc4I CCNNNNNNNGG 6 cut(s) 229, 614, 727, 948, 1500, 1511
Bse118I RCCGGY 3 cut(s) 194, 233, 571
Bse1I ACTGG 3 cut(s) 792, 942, 1523
Bse21I CCTNAGG 1 cut(s) 1174
Bse3DI GCAATG 3 cut(s) 490, 631, 1484
BseBI CCWGG 2 cut(s) 609, 830
BseDI CCNNGG 3 cut(s) 307, 829, 1145
BseGI GGATG 8 cut(s) 71, 119, 721, 1240, 1429, 1445, 1520, 1594
BseLI CCNNNNNNNGG 6 cut(s) 229, 614, 727, 948, 1500, 1511
BseMI GCAATG 3 cut(s) 490, 631, 1484
BseMII CTCAG 5 cut(s) 680, 1017, 1188, 1394, 1404
BseNI ACTGG 3 cut(s) 792, 942, 1523
BseRI GAGGAG 2 cut(s) 115, 1152
BseSI GKGCMC 1 cut(s) 1474
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 3 cut(s) 485, 596, 1432
BseYI CCCAGC 2 cut(s) 493, 947
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 4 cut(s) 824, 1335, 1337, 1368
Bsh1285I CGRYCG 2 cut(s) 234, 564
BshFI GGCC 7 cut(s) 36, 68, 233, 1144, 1150, 1504, 1580
BshNI GGYRCC 4 cut(s) 58, 350, 952, 1314
BsiEI CGRYCG 2 cut(s) 234, 564
BsiHKAI GWGCWC 6 cut(s) 218, 398, 905, 967, 1058, 1474
BsiSI CCGG 6 cut(s) 195, 234, 240, 399, 564, 572
BslFI GGGAC 2 cut(s) 1177, 1323
BslI CCNNNNNNNGG 6 cut(s) 229, 614, 727, 948, 1500, 1511
BsmFI GGGAC 2 cut(s) 1177, 1323
BsnI GGCC 7 cut(s) 36, 68, 233, 1144, 1150, 1504, 1580
Bsp119I TTCGAA 1 cut(s) 461
Bsp1286I GDGCHC 6 cut(s) 218, 398, 905, 967, 1058, 1474
Bsp143I GATC 6 cut(s) 131, 187, 499, 925, 1036, 1067
BspACI CCGC 8 cut(s) 69, 138, 165, 243, 273, 276, 291, 1103
BspANI GGCC 7 cut(s) 36, 68, 233, 1144, 1150, 1504, 1580
BspCNI CTCAG 5 cut(s) 679, 1016, 1187, 1393, 1405
BspFNI CGCG 4 cut(s) 824, 1335, 1337, 1368
BspLI GGNNCC 9 cut(s) 60, 238, 313, 352, 954, 1107, 1151, 1316, 1521
BspMAI CTGCAG 2 cut(s) 1450, 1648
BspMI ACCTGC 1 cut(s) 498
BspQI GCTCTTC 1 cut(s) 73
BspT104I TTCGAA 1 cut(s) 461
BspT107I GGYRCC 4 cut(s) 58, 350, 952, 1314
BspTI CTTAAG 1 cut(s) 1667
BsrDI GCAATG 3 cut(s) 490, 631, 1484
BsrFI RCCGGY 3 cut(s) 194, 233, 571
BsrI ACTGG 3 cut(s) 792, 942, 1523
BssAI RCCGGY 3 cut(s) 194, 233, 571
BssECI CCNNGG 3 cut(s) 307, 829, 1145
BssMI GATC 6 cut(s) 131, 187, 499, 925, 1036, 1067
BssNI GRCGYC 2 cut(s) 59, 110
BssT1I CCWWGG 2 cut(s) 307, 1145
Bst2UI CCWGG 2 cut(s) 609, 830
Bst4CI ACNGT 3 cut(s) 679, 1277, 1511
Bst6I CTCTTC 2 cut(s) 73, 1389
BstACI GRCGYC 2 cut(s) 59, 110
BstAFI CTTAAG 1 cut(s) 1667
BstBI TTCGAA 1 cut(s) 461
BstC8I GCNNGC 4 cut(s) 235, 573, 1344, 1470
BstDEI CTNAG 9 cut(s) 666, 690, 733, 769, 1003, 1174, 1380, 1413, 1707
BstF5I GGATG 8 cut(s) 71, 119, 721, 1240, 1429, 1445, 1520, 1594
BstFNI CGCG 4 cut(s) 824, 1335, 1337, 1368
BstH2I RGCGCY 2 cut(s) 62, 869
BstHHI GCGC 4 cut(s) 61, 824, 868, 1337
BstKTI GATC 6 cut(s) 134, 190, 502, 928, 1039, 1070
BstMBI GATC 6 cut(s) 131, 187, 499, 925, 1036, 1067
BstMCI CGRYCG 2 cut(s) 234, 564
BstMWI GCNNNNNNNGC 6 cut(s) 65, 270, 581, 1343, 1381, 1424
BstNI CCWGG 2 cut(s) 609, 830
BstPAI GACNNNNGTC 1 cut(s) 657
BstSCI CCNGG 2 cut(s) 607, 828
BstSFI CTRYAG 3 cut(s) 918, 1446, 1644
BstSLI GKGCMC 1 cut(s) 1474
BstUI CGCG 4 cut(s) 824, 1335, 1337, 1368
BstV1I GCAGC 3 cut(s) 485, 596, 1432
BstV2I GAAGAC 3 cut(s) 906, 1473, 1619
BstX2I RGATCY 1 cut(s) 131
BstYI RGATCY 1 cut(s) 131
BstZI CGGCCG 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 1174
BsuRI GGCC 7 cut(s) 36, 68, 233, 1144, 1150, 1504, 1580
BtsCI GGATG 8 cut(s) 71, 119, 721, 1240, 1429, 1445, 1520, 1594
BtsI GCAGTG 2 cut(s) 1081, 1353
BtsIMutI CAGTG 3 cut(s) 949, 1081, 1353
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 4 cut(s) 235, 573, 1344, 1470
CfoI GCGC 4 cut(s) 61, 824, 868, 1337
Cfr10I RCCGGY 3 cut(s) 194, 233, 571
Cfr13I GGNCC 5 cut(s) 1105, 1142, 1149, 1170, 1680
Csp6I GTAC 4 cut(s) 531, 663, 1079, 1377
CviAII CATG 4 cut(s) 334, 1016, 1048, 1183
CviQI GTAC 4 cut(s) 531, 663, 1079, 1377
DdeI CTNAG 9 cut(s) 666, 690, 733, 769, 1003, 1174, 1380, 1413, 1707
DinI GGCGCC 1 cut(s) 60
DpnI GATC 6 cut(s) 133, 189, 501, 927, 1038, 1069
DpnII GATC 6 cut(s) 131, 187, 499, 925, 1036, 1067
DraIII CACNNNGTG 1 cut(s) 1587
EaeI YGGCCR 2 cut(s) 66, 231
EagI CGGCCG 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 73, 1389
EarI CTCTTC 2 cut(s) 73, 1389
EciI GGCGGA 3 cut(s) 154, 258, 291
Ecl136II GAGCTC 3 cut(s) 216, 396, 903
EclXI CGGCCG 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 307, 1145
Eco24I GRGCYC 3 cut(s) 218, 398, 905
Eco47I GGWCC 3 cut(s) 1105, 1170, 1680
Eco52I CGGCCG 1 cut(s) 231
Eco53kI GAGCTC 3 cut(s) 216, 396, 903
Eco57I CTGAAG 1 cut(s) 685
Eco81I CCTNAGG 1 cut(s) 1174
EcoICRI GAGCTC 3 cut(s) 216, 396, 903
EcoO109I RGGNCCY 3 cut(s) 1142, 1149, 1170
EcoRI GAATTC 1 cut(s) 8
EcoRII CCWGG 2 cut(s) 607, 828
EcoT14I CCWWGG 2 cut(s) 307, 1145
EcoT22I ATGCAT 1 cut(s) 121
EcoT38I GRGCYC 3 cut(s) 218, 398, 905
EgeI GGCGCC 1 cut(s) 60
EheI GGCGCC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 307, 1145
FaeI CATG 4 cut(s) 337, 1019, 1051, 1186
FalI AAGNNNNNCTT 4 cut(s) 683, 715, 1147, 1179
FaqI GGGAC 2 cut(s) 1177, 1323
FatI CATG 4 cut(s) 333, 1015, 1047, 1182
FauI CCCGC 1 cut(s) 298
Fnu4HI GCNGC 5 cut(s) 69, 274, 474, 585, 1446
FokI GGATG 8 cut(s) 58, 106, 728, 1247, 1436, 1452, 1527, 1581
FriOI GRGCYC 3 cut(s) 218, 398, 905
Fsp4HI GCNGC 5 cut(s) 69, 274, 474, 585, 1446
FspBI CTAG 7 cut(s) 285, 1011, 1040, 1071, 1146, 1167, 1623
GlaI GCGC 4 cut(s) 60, 823, 867, 1336
GluI GCNGC 5 cut(s) 69, 274, 474, 585, 1446
GsaI CCCAGC 2 cut(s) 497, 951
GsuI CTGGAG 1 cut(s) 837
HaeII RGCGCY 2 cut(s) 62, 869
HaeIII GGCC 7 cut(s) 36, 68, 233, 1144, 1150, 1504, 1580
HapII CCGG 6 cut(s) 195, 234, 240, 399, 564, 572
HhaI GCGC 4 cut(s) 61, 824, 868, 1337
Hin1I GRCGYC 2 cut(s) 59, 110
Hin1II CATG 4 cut(s) 337, 1019, 1051, 1186
Hin6I GCGC 4 cut(s) 59, 822, 866, 1335
HinP1I GCGC 4 cut(s) 59, 822, 866, 1335
HincII GTYRAC 1 cut(s) 1273
HindII GTYRAC 1 cut(s) 1273
HindIII AAGCTT 1 cut(s) 1674
HinfI GANTC 7 cut(s) 246, 279, 329, 590, 737, 764, 998
HpaII CCGG 6 cut(s) 195, 234, 240, 399, 564, 572
HphI GGTGA 3 cut(s) 509, 699, 1289
Hpy166II GTNNAC 5 cut(s) 707, 1079, 1273, 1472, 1570
Hpy188I TCNGA 4 cut(s) 316, 480, 595, 784
Hpy188III TCNNGA 7 cut(s) 75, 129, 182, 816, 929, 1040, 1598
Hpy8I GTNNAC 5 cut(s) 707, 1079, 1273, 1472, 1570
Hpy99I CGWCG 7 cut(s) 112, 115, 188, 263, 364, 367, 377
HpyAV CCTTC 5 cut(s) 983, 987, 1206, 1451, 1645
HpyCH4III ACNGT 3 cut(s) 679, 1277, 1511
HpyCH4IV ACGT 3 cut(s) 110, 258, 359
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 270, 581, 1343, 1381, 1424
HpyF3I CTNAG 9 cut(s) 666, 690, 733, 769, 1003, 1174, 1380, 1413, 1707
HpySE526I ACGT 3 cut(s) 110, 258, 359
Hsp92I GRCGYC 2 cut(s) 59, 110
Hsp92II CATG 4 cut(s) 337, 1019, 1051, 1186
HspAI GCGC 4 cut(s) 59, 822, 866, 1335
KasI GGCGCC 1 cut(s) 58
KroI GCCGGC 2 cut(s) 233, 571
KroNI GCCGGC 2 cut(s) 235, 573
Kzo9I GATC 6 cut(s) 131, 187, 499, 925, 1036, 1067
LguI GCTCTTC 1 cut(s) 73
Lsp1109I GCAGC 3 cut(s) 485, 596, 1432
LweI GCATC 5 cut(s) 80, 128, 273, 1108, 1414
MaeI CTAG 7 cut(s) 285, 1011, 1040, 1071, 1146, 1167, 1623
MaeII ACGT 3 cut(s) 110, 258, 359
MaeIII GTNAC 3 cut(s) 280, 603, 983
MalI GATC 6 cut(s) 133, 189, 501, 927, 1038, 1069
MboI GATC 6 cut(s) 131, 187, 499, 925, 1036, 1067
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 6 cut(s) 218, 398, 905, 967, 1058, 1474
Mly113I GGCGCC 1 cut(s) 59
MlyI GAGTC 1 cut(s) 288
MmeI TCCRAC 1 cut(s) 339
Mph1103I ATGCAT 1 cut(s) 121
MreI CGCCGGCG 1 cut(s) 571
MroNI GCCGGC 2 cut(s) 233, 571
MseI TTAA 6 cut(s) 321, 411, 681, 1095, 1560, 1668
MspA1I CMGCKG 1 cut(s) 587
MspCI CTTAAG 1 cut(s) 1667
MspI CCGG 6 cut(s) 195, 234, 240, 399, 564, 572
MspR9I CCNGG 2 cut(s) 609, 830
MvaI CCWGG 2 cut(s) 609, 830
MvnI CGCG 4 cut(s) 824, 1335, 1337, 1368
MwoI GCNNNNNNNGC 6 cut(s) 65, 270, 581, 1343, 1381, 1424
NaeI GCCGGC 2 cut(s) 235, 573
NarI GGCGCC 1 cut(s) 59
NdeII GATC 6 cut(s) 131, 187, 499, 925, 1036, 1067
NgoMIV GCCGGC 2 cut(s) 233, 571
NlaIII CATG 4 cut(s) 337, 1019, 1051, 1186
NlaIV GGNNCC 9 cut(s) 60, 238, 313, 352, 954, 1107, 1151, 1316, 1521
NmuCI GTSAC 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 121
NspV TTCGAA 1 cut(s) 461
PciSI GCTCTTC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 1372
PdiI GCCGGC 2 cut(s) 235, 573
PfeI GAWTC 6 cut(s) 246, 329, 590, 737, 764, 998
PkrI GCNGC 5 cut(s) 70, 275, 475, 586, 1447
PleI GAGTC 1 cut(s) 287
PluTI GGCGCC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 287
PpuMI RGGWCCY 1 cut(s) 1170
PshAI GACNNNNGTC 1 cut(s) 657
Psp124BI GAGCTC 3 cut(s) 218, 398, 905
Psp5II RGGWCCY 1 cut(s) 1170
Psp6I CCWGG 2 cut(s) 607, 828
PspFI CCCAGC 2 cut(s) 493, 947
PspGI CCWGG 2 cut(s) 607, 828
PspN4I GGNNCC 9 cut(s) 60, 238, 313, 352, 954, 1107, 1151, 1316, 1521
PspPI GGNCC 5 cut(s) 1105, 1142, 1149, 1170, 1680
PspPPI RGGWCCY 1 cut(s) 1170
PstI CTGCAG 2 cut(s) 1450, 1648
PsuI RGATCY 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 4 cut(s) 532, 664, 1080, 1378
RsaNI GTAC 4 cut(s) 531, 663, 1079, 1377
SacI GAGCTC 3 cut(s) 218, 398, 905
SapI GCTCTTC 1 cut(s) 73
SaqAI TTAA 6 cut(s) 321, 411, 681, 1095, 1560, 1668
SatI GCNGC 5 cut(s) 69, 274, 474, 585, 1446
Sau3AI GATC 6 cut(s) 131, 187, 499, 925, 1036, 1067
Sau96I GGNCC 5 cut(s) 1105, 1142, 1149, 1170, 1680
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 2 cut(s) 609, 830
SduI GDGCHC 6 cut(s) 218, 398, 905, 967, 1058, 1474
SfaNI GCATC 5 cut(s) 80, 128, 273, 1108, 1414
SfcI CTRYAG 3 cut(s) 918, 1446, 1644
SfoI GGCGCC 1 cut(s) 60
SfuI TTCGAA 1 cut(s) 461
SgrAI CRCCGGYG 1 cut(s) 571
SinI GGWCC 3 cut(s) 1105, 1170, 1680
SmlI CTYRAG 4 cut(s) 127, 882, 929, 1667
SmoI CTYRAG 4 cut(s) 127, 882, 929, 1667
SsiI CCGC 8 cut(s) 69, 138, 165, 243, 273, 276, 291, 1103
SspDI GGCGCC 1 cut(s) 58
SspI AATATT 1 cut(s) 1113
SspMI CTAG 7 cut(s) 285, 1011, 1040, 1071, 1146, 1167, 1623
SstI GAGCTC 3 cut(s) 218, 398, 905
StyD4I CCNGG 2 cut(s) 607, 828
StyI CCWWGG 2 cut(s) 307, 1145
TaaI ACNGT 3 cut(s) 679, 1277, 1511
TaiI ACGT 3 cut(s) 113, 261, 362
TaqI TCGA 5 cut(s) 183, 212, 461, 752, 1066
TauI GCSGC 2 cut(s) 71, 276
TfiI GAWTC 6 cut(s) 246, 329, 590, 737, 764, 998
Tru1I TTAA 6 cut(s) 321, 411, 681, 1095, 1560, 1668
Tru9I TTAA 6 cut(s) 321, 411, 681, 1095, 1560, 1668
TscAI CASTG 3 cut(s) 949, 1081, 1353
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 3 cut(s) 473, 584, 1445
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 5 cut(s) 828, 1036, 1199, 1373, 1473
TspGWI ACGGA 3 cut(s) 38, 1142, 1483
TspRI CASTG 3 cut(s) 949, 1081, 1353
Vha464I CTTAAG 1 cut(s) 1667
VneI GTGCAC 1 cut(s) 1470
VpaK11BI GGWCC 3 cut(s) 1105, 1170, 1680
XapI RAATTY 3 cut(s) 8, 935, 1259
XbaI TCTAGA 1 cut(s) 1039
XmaJI CCTAGG 1 cut(s) 1145
XspI CTAG 7 cut(s) 285, 1011, 1040, 1071, 1146, 1167, 1623
ZraI GACGTC 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.