pycom08g08000

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013469
Physical Location & Seq
Reverse (-)
6445386 .. 6446451
1066 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
ATGGCGTTGATGATAGACAACTGCGAGAGCATACTGCTCTCCCTGGACTCCCACAAGTCCGTCCCGGCGCCGTTCCTGACAAAAACCTACCAACTCGTCGACGACCCCACCACCGACCATATCGTCTCGTGGGGCGACGACGACACCACCTTCGTCGTCTGGCGCCCTCCCGAGTTCGCCCGCGACCTCCTCCCCAACTACTTCAAGCACAACAACTTCTCCAGCTTCGTCCGCCAGCTAAACACCTACGGTTTTAGGAAGATTGTACCGGACAGATGGGAGTTTGCCAACGAGTTCTTCAAAAAAGGAGAGAAGCATTTGCTTTGCGAGATCCACCGGAGAAAGACAGCTCAGCCACATCAGGTGGGTCTCAACCACCACCACCACCACCACCACCACTCACCCCTCGGCATGAACGGCCACCACCACCATCCTAGCTTCTTCTCCTTCCCGAGTCGCGGCAGCATCTCGCCCTCCGACTCGGACGAGCAGCCCAACTGGTGCGAGTCGGACTCCCCTCCTCTCTTATCCCCAACCGGAGGTATTAACACTAACAACAATAACTTCATGAATATTAACCATAATAATACCACGGTGGCGGGTTTGGCGGAGGACAACGAGAGGCTGCGGAGGAGCAACAACATGCTAATGTCTGAGCTGACACACATGAGAAAACTCTACAACGACATCATCTACTTTGTCCAGAACCACGTCAAGCCGGTGGCTCCGAGCAACTCCTACCCTTCTTCAATGCTTCTCTGCAACCCACAGCCTCCTAAACCTAATGGTCTTAATGGTAATTTAAACCAACTTCAAGGGTACTATCCGGCTGCTCCTCCACCTAATGCCAAACAAAACCCTCATCACATCATGAACTCTTCCAGTCCGATGAGCAACACCACGTCGAAGAGCTCCGTGACGATTCTCGAAGATCGACCACCAACAGCCGCCGAGCAGTAA

Protein Analysis

320

Amino Acids

36.23

Weight (kDa)

6.76

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 25 - 114 9.1e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G46264
fragaria_vesca FvH4_2g34260
malus_domestica MD08G1098200.v1.1 MD15G1080700.v1.1
prunus_persica Prupe.1G433500_v2.0.a1
pyrus_communis pycom08g08000 pycom15g07520
rosa_chinensis RchiOBHm_Chr6g0308161
rosa_laevigata RLG00000010664
rosa_multiflora Rmu_sc0005706.1_g000009
rosa_roxburghii Rroxscaffold_7G00160400 Rroxscaffold_7G00160490
rosa_rugosa Rorug06G0364300
rosa_samantha Rh6AG473500 Rh6BG484300 Rh6CG489300 Rh6DG475500
rosa_wichuraiana Rw6G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AccB1I GGYRCC 2 cut(s) 67, 162
AccI GTMKAC 1 cut(s) 99
AccII CGCG 2 cut(s) 183, 459
AciI CCGC 7 cut(s) 181, 232, 459, 599, 608, 628, 948
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 1 cut(s) 418
AcyI GRCGYC 2 cut(s) 68, 163
AfaI GTAC 2 cut(s) 267, 821
AfiI CCNNNNNNNGG 2 cut(s) 458, 539
AgsI TTSAA 4 cut(s) 205, 301, 750, 815
AjiI CACGTC 2 cut(s) 712, 903
AjnI CCWGG 1 cut(s) 42
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AluBI AGCT 6 cut(s) 225, 238, 350, 438, 658, 912
AluI AGCT 6 cut(s) 225, 238, 350, 438, 658, 912
Alw21I GWGCWC 1 cut(s) 914
Alw26I GTCTC 2 cut(s) 130, 374
AlwI GGATC 1 cut(s) 325
Ama87I CYCGRG 2 cut(s) 170, 451
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 4 cut(s) 462, 490, 625, 830
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspLEI GCGC 2 cut(s) 70, 165
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 393
AvaI CYCGRG 2 cut(s) 170, 451
BanI GGYRCC 2 cut(s) 67, 162
BanII GRGCYC 1 cut(s) 914
BauI CACGAG 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 914
BbvI GCAGC 4 cut(s) 474, 502, 612, 817
BccI CCATC 2 cut(s) 270, 438
BceAI ACGGC 2 cut(s) 55, 433
BciT130I CCWGG 1 cut(s) 44
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 2 cut(s) 130, 374
BfaI CTAG 1 cut(s) 435
BfoI RGCGCY 2 cut(s) 71, 166
BisI GCNGC 6 cut(s) 460, 463, 491, 626, 831, 948
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 6 cut(s) 461, 464, 492, 627, 832, 949
Bme1390I CCNGG 2 cut(s) 44, 65
BmeT110I CYCGRG 2 cut(s) 170, 451
BmgBI CACGTC 2 cut(s) 712, 903
BmiI GGNNCC 3 cut(s) 69, 164, 726
BmrFI CCNGG 2 cut(s) 44, 65
BmsI GCATC 1 cut(s) 474
BplI GAGNNNNNCTC 2 cut(s) 497, 529
BpmI CTGGAG 1 cut(s) 205
Bpu1102I GCTNAGC 1 cut(s) 351
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 2 cut(s) 68, 163
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 42, 406, 591
BsaWI WCCGGW 3 cut(s) 268, 336, 536
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 539
Bse118I RCCGGY 1 cut(s) 718
Bse1I ACTGG 2 cut(s) 503, 882
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 42, 406, 591
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 2 cut(s) 458, 539
BseMII CTCAG 2 cut(s) 365, 645
BseNI ACTGG 2 cut(s) 503, 882
BseRI GAGGAG 4 cut(s) 179, 510, 646, 825
BseXI GCAGC 4 cut(s) 474, 502, 612, 817
Bsh1236I CGCG 2 cut(s) 183, 459
BshFI GGCC 1 cut(s) 420
BshNI GGYRCC 2 cut(s) 67, 162
BsiHKAI GWGCWC 1 cut(s) 914
BsiHKCI CYCGRG 2 cut(s) 170, 451
BsiSI CCGG 6 cut(s) 65, 269, 337, 537, 719, 827
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 458, 539
BsmAI GTCTC 2 cut(s) 130, 374
BsmBI CGTCTC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 47
BsnI GGCC 1 cut(s) 420
Bso31I GGTCTC 1 cut(s) 374
BsoBI CYCGRG 2 cut(s) 170, 451
Bsp1286I GDGCHC 1 cut(s) 914
Bsp143I GATC 2 cut(s) 330, 931
Bsp1720I GCTNAGC 1 cut(s) 351
BspACI CCGC 7 cut(s) 181, 232, 459, 599, 608, 628, 948
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 2 cut(s) 364, 646
BspFNI CGCG 2 cut(s) 183, 459
BspHI TCATGA 2 cut(s) 567, 870
BspLI GGNNCC 3 cut(s) 69, 164, 726
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 902
BspT107I GGYRCC 2 cut(s) 67, 162
BspTNI GGTCTC 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 718
BsrI ACTGG 2 cut(s) 503, 882
BssAI RCCGGY 1 cut(s) 718
BssECI CCNNGG 3 cut(s) 42, 406, 591
BssMI GATC 2 cut(s) 330, 931
BssNI GRCGYC 2 cut(s) 68, 163
BssSI CACGAG 1 cut(s) 127
Bst2BI CACGAG 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 251, 595
Bst6I CTCTTC 2 cut(s) 883, 902
BstACI GRCGYC 2 cut(s) 68, 163
BstC8I GCNNGC 2 cut(s) 181, 236
BstDEI CTNAG 2 cut(s) 351, 654
BstDSI CCRYGG 1 cut(s) 591
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 2 cut(s) 183, 459
BstH2I RGCGCY 2 cut(s) 71, 166
BstHHI GCGC 2 cut(s) 70, 165
BstKTI GATC 2 cut(s) 333, 934
BstMAI GTCTC 2 cut(s) 130, 374
BstMBI GATC 2 cut(s) 330, 931
BstMWI GCNNNNNNNGC 3 cut(s) 231, 417, 605
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 2 cut(s) 42, 63
BstUI CGCG 2 cut(s) 183, 459
BstV1I GCAGC 4 cut(s) 474, 502, 612, 817
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BsuRI GGCC 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 591
BtrI CACGTC 2 cut(s) 712, 903
BtsCI GGATG 1 cut(s) 430
Cac8I GCNNGC 2 cut(s) 181, 236
CciI TCATGA 2 cut(s) 567, 870
CfoI GCGC 2 cut(s) 70, 165
Cfr10I RCCGGY 1 cut(s) 718
Csp6I GTAC 2 cut(s) 266, 820
CviAII CATG 5 cut(s) 412, 568, 643, 667, 871
CviQI GTAC 2 cut(s) 266, 820
DdeI CTNAG 2 cut(s) 351, 654
DinI GGCGCC 2 cut(s) 69, 164
DpnI GATC 2 cut(s) 332, 933
DpnII GATC 2 cut(s) 330, 931
DraI TTTAAA 1 cut(s) 804
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 418
Eam1104I CTCTTC 2 cut(s) 883, 902
EarI CTCTTC 2 cut(s) 883, 902
EciI GGCGGA 2 cut(s) 221, 623
Ecl136II GAGCTC 1 cut(s) 912
Eco24I GRGCYC 1 cut(s) 914
Eco31I GGTCTC 1 cut(s) 374
Eco53kI GAGCTC 1 cut(s) 912
Eco88I CYCGRG 2 cut(s) 170, 451
EcoICRI GAGCTC 1 cut(s) 912
EcoRII CCWGG 1 cut(s) 42
EcoT38I GRGCYC 1 cut(s) 914
EgeI GGCGCC 2 cut(s) 69, 164
EheI GGCGCC 2 cut(s) 69, 164
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 5 cut(s) 415, 571, 646, 670, 874
FaiI YATR 8 cut(s) 32, 120, 413, 569, 582, 644, 668, 872
FaqI GGGAC 1 cut(s) 47
FatI CATG 5 cut(s) 411, 567, 642, 666, 870
FauI CCCGC 2 cut(s) 188, 592
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 6 cut(s) 460, 463, 491, 626, 831, 948
FokI GGATG 1 cut(s) 417
FriOI GRGCYC 1 cut(s) 914
Fsp4HI GCNGC 6 cut(s) 460, 463, 491, 626, 831, 948
FspBI CTAG 1 cut(s) 435
GlaI GCGC 2 cut(s) 69, 164
GluI GCNGC 6 cut(s) 460, 463, 491, 626, 831, 948
GsuI CTGGAG 1 cut(s) 205
HaeII RGCGCY 2 cut(s) 71, 166
HaeIII GGCC 1 cut(s) 420
HapII CCGG 6 cut(s) 65, 269, 337, 537, 719, 827
HhaI GCGC 2 cut(s) 70, 165
Hin1I GRCGYC 2 cut(s) 68, 163
Hin1II CATG 5 cut(s) 415, 571, 646, 670, 874
Hin6I GCGC 2 cut(s) 68, 163
HinP1I GCGC 2 cut(s) 68, 163
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 6 cut(s) 47, 454, 479, 506, 512, 922
HpaII CCGG 6 cut(s) 65, 269, 337, 537, 719, 827
HphI GGTGA 1 cut(s) 393
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 6 cut(s) 478, 484, 511, 655, 729, 888
Hpy188III TCNNGA 7 cut(s) 76, 170, 451, 568, 703, 871, 926
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 6 cut(s) 101, 104, 140, 143, 158, 907
HpyAV CCTTC 3 cut(s) 160, 457, 753
HpyCH4III ACNGT 2 cut(s) 251, 595
HpyCH4IV ACGT 2 cut(s) 711, 902
HpyCH4V TGCA 1 cut(s) 762
HpyF10VI GCNNNNNNNGC 3 cut(s) 231, 417, 605
HpyF3I CTNAG 2 cut(s) 351, 654
HpySE526I ACGT 2 cut(s) 711, 902
Hsp92I GRCGYC 2 cut(s) 68, 163
Hsp92II CATG 5 cut(s) 415, 571, 646, 670, 874
HspAI GCGC 2 cut(s) 68, 163
KasI GGCGCC 2 cut(s) 67, 162
Kzo9I GATC 2 cut(s) 330, 931
LguI GCTCTTC 1 cut(s) 902
LmnI GCTCC 4 cut(s) 633, 730, 838, 917
Lsp1109I GCAGC 4 cut(s) 474, 502, 612, 817
LweI GCATC 1 cut(s) 474
MaeI CTAG 1 cut(s) 435
MaeII ACGT 2 cut(s) 711, 902
MaeIII GTNAC 1 cut(s) 916
MalI GATC 2 cut(s) 332, 933
MboI GATC 2 cut(s) 330, 931
MboII GAAGA 7 cut(s) 271, 289, 433, 738, 870, 919, 941
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 1 cut(s) 914
MluCI AATT 1 cut(s) 799
Mly113I GGCGCC 2 cut(s) 68, 163
MlyI GAGTC 5 cut(s) 41, 463, 473, 506, 515
MmeI TCCRAC 2 cut(s) 489, 501
MseI TTAA 4 cut(s) 546, 576, 792, 803
MslI CAYNNNNRTG 1 cut(s) 647
MspI CCGG 6 cut(s) 65, 269, 337, 537, 719, 827
MspR9I CCNGG 2 cut(s) 44, 65
MvaI CCWGG 1 cut(s) 44
MvnI CGCG 2 cut(s) 183, 459
MwoI GCNNNNNNNGC 3 cut(s) 231, 417, 605
NarI GGCGCC 2 cut(s) 68, 163
NciI CCSGG 1 cut(s) 65
NdeII GATC 2 cut(s) 330, 931
NlaIII CATG 5 cut(s) 415, 571, 646, 670, 874
NlaIV GGNNCC 3 cut(s) 69, 164, 726
NmeAIII GCCGAG 1 cut(s) 387
NmuCI GTSAC 1 cut(s) 916
NspI RCATGY 1 cut(s) 646
PagI TCATGA 2 cut(s) 567, 870
PciSI GCTCTTC 1 cut(s) 902
PfeI GAWTC 1 cut(s) 922
PkrI GCNGC 6 cut(s) 461, 464, 492, 627, 832, 949
PleI GAGTC 5 cut(s) 41, 462, 473, 506, 514
PluTI GGCGCC 2 cut(s) 71, 166
PpsI GAGTC 5 cut(s) 41, 462, 473, 506, 514
Psp124BI GAGCTC 1 cut(s) 914
Psp6I CCWGG 1 cut(s) 42
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 3 cut(s) 69, 164, 726
PsuI RGATCY 1 cut(s) 330
RsaI GTAC 2 cut(s) 267, 821
RsaNI GTAC 2 cut(s) 266, 820
RseI CAYNNNNRTG 1 cut(s) 647
SacI GAGCTC 1 cut(s) 914
SalI GTCGAC 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 902
SaqAI TTAA 4 cut(s) 546, 576, 792, 803
SatI GCNGC 6 cut(s) 460, 463, 491, 626, 831, 948
Sau3AI GATC 2 cut(s) 330, 931
SchI GAGTC 5 cut(s) 41, 463, 473, 506, 515
ScrFI CCNGG 2 cut(s) 44, 65
SduI GDGCHC 1 cut(s) 914
SfaNI GCATC 1 cut(s) 474
SfoI GGCGCC 2 cut(s) 69, 164
SgrDI CGTCGACG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 647
Sse9I AATT 1 cut(s) 799
SsiI CCGC 7 cut(s) 181, 232, 459, 599, 608, 628, 948
SspDI GGCGCC 2 cut(s) 67, 162
SspI AATATT 1 cut(s) 574
SspMI CTAG 1 cut(s) 435
SstI GAGCTC 1 cut(s) 914
StyD4I CCNGG 2 cut(s) 42, 63
TaaI ACNGT 2 cut(s) 251, 595
TaiI ACGT 2 cut(s) 714, 905
TaqI TCGA 4 cut(s) 99, 905, 927, 934
TasI AATT 1 cut(s) 799
TauI GCSGC 2 cut(s) 462, 950
TfiI GAWTC 1 cut(s) 922
Tru1I TTAA 4 cut(s) 546, 576, 792, 803
Tru9I TTAA 4 cut(s) 546, 576, 792, 803
TseFI GTSAC 1 cut(s) 916
TseI GCWGC 4 cut(s) 462, 490, 625, 830
Tsp45I GTSAC 1 cut(s) 916
TspDTI ATGAA 4 cut(s) 428, 556, 584, 887
TspGWI ACGGA 2 cut(s) 49, 904
XceI RCATGY 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.