Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0014256

Physical Location & Seq

Genomic Coordinates Forward (+)

8937187 .. 8943403

Gene Structure 6217 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2475 bp

ATGCAAACCTTCTCGGCCTCCTTCGCCGTCTGTACTTCCCACCCATTACGACGCCGTCGAATCAGCACCTCCCCTTCCCGCAAACCCAATCTCCGGCGACTCACCTCCCGCGTTGTCCAGCTCACCCGCCGGAGACAGCTTCACCAGATAATGGAGGAGATAGAGGTCGCCAAAGAACGATTCGGGAAGCTGAACACGGTTGTGATGAACGCGGTCGTGCAGGCTTGTGTACATTGCGGCGATATTGACTCTGCACTCATGTTGTTCGACGAAATGGCTGAGCCAGGAAGCTGTGGCGTTGATACTATCACATATGGAACCATCTTAAAGGGTTTGGGCGCGGCACGGAGAATTGATGAAGCATTTTATGTACTTGAATCTGTGGAGCGAGGCACTGCAGTGGGAAGTCCAAAATTGTCAGCACCTCTTGTTCTTGGTCTGCTCAATGCTCTAGTTGAAGCAGGAGATTTACGCCGTGCCAATGGGCTTCTTCATCACTATGGTTTTCTCCTTCGTGAAGGTGGCAGTCTTACAGTCAGAGTCTACAACTTACTAATGAAGGGATATATCAATGCAGGCCTTCCTCAGGGTGCAATAACCATGCACGACAAGATATTACGTCTAGGATTAAAGCCTGATAAGCTCACCTATAATACTCTGATATCTGCATGTGTAAAAGCTGGAAAGTTGGACATTGCAATGCAGTTTTTTGAGGAAATGAAGGATGAAGCACAGAAATTTAGCCATGGTGACCTTTTCCCAGATGTGGTCACTTACACAACATTACTTAAGGGTTTTGGGGATTCCAAAGATATTAATTCAGTTCAGAAGATCATATTGGAAATGAAAACATATATTGGTTTGTTCATTGATCGAACAGCATACACTGCAGCAGCTGATGCTTTGCTAAATTGTGGCTCAATAAAAGGTGCTTTATGCATATTGGGAGAAATATTAAAGCGGGCTGGGGGAAAGCCAAATTTACGACCAAAACCTCACCTCTATCTTTCCTTCATGCGCTCATTTTCTGTTAGTGGAGATTACAGTACCGTTGAAAATCTAAATGAGCACATGTGGCGGGATACTTCTGGAACTATCTCCCCAATGGTTCAAGAGGAAGCTGATCATCTACTCATGGAGGCAGCTTTAAATGGTGGTCAGGTAGATTTGGCTGTAAAATACCTCGCAAGTACTATTACAAGGTGGAAGAGGATATCATGGACAAGTCGAGGAGGCATGGTGGCTCTCCGCATAGAGGCCTTGCTGGGATTCACCAAATCGATATTGAGTCCTTATCTACTTCCTCAGGTCTTGGCAAGTGAACCAATTGAAAGTGTCATGATGCCCTTTGAAGCGGCTCAGCCGCTTAATGGGACCCTGGACTTGAACAAAGTGGCGATGCGTTTCTTTAAGGATTCAGTGGTGCCCATCATAGATGACTGGGGTAGCTGCATTGGCATATTGCACCGTGAAGACTGTCGAGAGATGAACGCCCCTCTATCGTCAATGATGAGAAGCCCGCCTCCTTGTGTTGCTACTCGAACGTCAATAGGGCATGTGGTGGATCTGATCATGAAGTACAGATATAAAATGGTGATTGTAATAAACCATAGCTCAGTATACGGTGGTAGTGCTTCAAGTTTGAGAGCTGCTGGTGTTTTTACAGCCGAACAACTGTGTAAACTTGTGTCACCGGAATCCGAAACGCCCGGTCTGAGTCGACTTTCTTTACGAATCGAAGACACAGTGGAATCAGAAATGGCGTTGGTGAGGGGGAAGGAGACGATCAAGAGCGTGATGAAGAAGTTCGGCGACAAAACCCTAACGCCGCGGCTGAAGGAGGAGCTGAAGAAGAAGGGCACCATGGGAGGGAAAACCCTACCCACCCGATTGAGAGAGGAGCTCAAGAAGAAGGGCATGCCCGATTCAAATATCATCATGGGTCGGGCCAAGCGCGGCATCTACGCCGGCCGCCACATCCAGTTCGGCAACCAAATCAGCGAAGACGGCGGCAACAAATCAAGGAGGACATGGAAGCCAAATGTGCAGGAGAAGCGGCTCTTCAGTTACATCCTAGACCGCCACATACGTGTCAAAGTCACCACCCATGCTCTCCGTTGCATAGACAAGGCAGGTGGGATCGATGAGTATTTTCTGAAAACACCTTACCAGAAGATGGATACGGAAATGGGATTTGTCTGGAAAGCCAGAATTGAAAAATTATATGAAGATGTTGGGCACAAGCAGGTTGCTTTTAATAAACCTGAAGCTGAATCCGAAGCCAACTTGGAACAAGGATTCAAAAACCTTACAATAGACAAAAGGGAAGTCAAGAGACAGATGTATGGTTGGTCGAAGAAACTAAAACAGATCGACGAAGGAACTGACAAACAAGTAGCTGATGTCGTAGATGCAAAGGTCGATGCAGGAAGCTCTCATGTTGGTGTTCCCGAGCAATTGGTCGCAAACTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS PPR PPR_2

Protein Analysis

825

Amino Acids

91.69

Weight (kDa)

9.33

Isoelectric Point (pI)

43.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	65 - 112	4.9e-08	PPR repeat family
PPR_2	PF13041	179 - 226	1.7e-09	PPR repeat family
PPR_3	PF13812	203 - 243	1.5e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	209 - 241	9.9e-11	PPR repeat
PPR_2	PF13041	216 - 244	4.1e-07	PPR repeat family
PPR	PF01535	216 - 242	6.8e-08	PPR repeat
Ribosomal_L28	PF00830	652 - 711	1.2e-21	Ribosomal L28 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014256)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10690 AT5G10690
fragaria_vesca	FvH4_2g36670 FvH4_2g36670
malus_domestica	MD08G1128000.v1.1
prunus_persica	Prupe.1G459800_v2.0.a1
pyrus_communis	pycom08g10700
rosa_chinensis	RchiOBHm_Chr6g0305021
rosa_laevigata	RLG00000010927
rosa_multiflora	Rmu_sc0000061.1_g000039
rosa_roxburghii	Rroxscaffold_7G00163250
rosa_rugosa	Rorug06G0336800
rosa_samantha	Rh6AG448400 Rh6BG452500 Rh6CG462100 Rh6DG449000
rosa_wichuraiana	Rw6G039050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2123
Acc36I	ACCTGC	2 cut(s)	2123, 2236
AccB1I	GGYRCC	2 cut(s)	1423, 1859
AccB7I	CCANNNNNTGG	2 cut(s)	151, 2176
AccI	GTMKAC	3 cut(s)	543, 1620, 1720
AccII	CGCG	5 cut(s)	111, 212, 341, 1831, 1956
AclWI	GGATC	2 cut(s)	1572, 2147
AcoI	YGGCCR	1 cut(s)	1969
AcsI	RAATTY	2 cut(s)	737, 979
AcuI	CTGAAG	4 cut(s)	1856, 1868, 2047, 2286
AcyI	GRCGYC	1 cut(s)	52
AfaI	GTAC	6 cut(s)	34, 231, 372, 1048, 1192, 1580
AfiI	CCNNNNNNNGG	8 cut(s)	93, 151, 586, 766, 1255, 1370, 1869, 2176
AflII	CTTAAG	1 cut(s)	788
AflIII	ACRYGT	2 cut(s)	1071, 2089
AjnI	CCWGG	2 cut(s)	283, 1377
AjuI	GAANNNNNNNTTGG	4 cut(s)	164, 196, 821, 853
AleI	CACNNNNGTG	3 cut(s)	200, 398, 2088
Alw21I	GWGCWC	2 cut(s)	1071, 1905
Alw26I	GTCTC	3 cut(s)	127, 1775, 2329
AlwI	GGATC	2 cut(s)	1572, 2147
AlwNI	CAGNNNCTG	1 cut(s)	896
Ama87I	CYCGRG	1 cut(s)	2450
AoxI	GGCC	5 cut(s)	15, 577, 1257, 1947, 1969
ApeKI	GCWGC	5 cut(s)	890, 893, 1142, 1449, 1649
ApoI	RAATTY	2 cut(s)	737, 979
ArsI	GACNNNNNNTTYG	2 cut(s)	2313, 2345
AseI	ATTAAT	1 cut(s)	816
Asp700I	GAANNNNTTC	1 cut(s)	1805
AspLEI	GCGC	3 cut(s)	341, 1020, 1956
AspS9I	GGNCC	2 cut(s)	1374, 1947
AsuC2I	CCSGG	1 cut(s)	1710
AvaI	CYCGRG	1 cut(s)	2450
AvaII	GGWCC	1 cut(s)	1374
AxyI	CCTNAGG	2 cut(s)	585, 1305
BaeGI	GKGCMC	3 cut(s)	1428, 1862, 2241
BanI	GGYRCC	2 cut(s)	1423, 1859
BanII	GRGCYC	1 cut(s)	1905
BbsI	GAAGAC	3 cut(s)	1479, 1746, 2010
Bbv12I	GWGCWC	2 cut(s)	1071, 1905
BbvI	GCAGC	5 cut(s)	902, 905, 1154, 1436, 1636
BccI	CCATC	3 cut(s)	329, 1436, 2170
BceAI	ACGGC	4 cut(s)	11, 39, 459, 2023
BciT130I	CCWGG	2 cut(s)	285, 1379
BciVI	GTATCC	2 cut(s)	1075, 2173
BclI	TGATCA	2 cut(s)	1123, 1569
BcnI	CCSGG	1 cut(s)	1710
BcoDI	GTCTC	3 cut(s)	127, 1775, 2329
BfaI	CTAG	3 cut(s)	452, 623, 2075
BfmI	CTRYAG	2 cut(s)	396, 888
BfrI	CTTAAG	1 cut(s)	788
BfuAI	ACCTGC	2 cut(s)	2123, 2236
BfuI	GTATCC	2 cut(s)	1075, 2173
BlpI	GCTNAGC	2 cut(s)	279, 1359
BmcAI	AGTACT	1 cut(s)	1192
Bme1390I	CCNGG	3 cut(s)	285, 1379, 1710
Bme18I	GGWCC	1 cut(s)	1374
BmeT110I	CYCGRG	1 cut(s)	2450
BmgT120I	GGNCC	2 cut(s)	1374, 1947
BmiI	GGNNCC	5 cut(s)	319, 1375, 1376, 1425, 1861
BmrFI	CCNGG	3 cut(s)	285, 1379, 1710
BmrI	ACTGGG	1 cut(s)	1450
BmsI	GCATC	6 cut(s)	889, 1332, 1389, 1968, 2401, 2413
BmuI	ACTGGG	1 cut(s)	1450
BpiI	GAAGAC	3 cut(s)	1479, 1746, 2010
BplI	GAGNNNNNCTC	2 cut(s)	1887, 1919
Bpu1102I	GCTNAGC	2 cut(s)	279, 1359
BpuEI	CTTGAG	1 cut(s)	1889
BpuMI	CCSGG	1 cut(s)	1710
Bsa29I	ATCGAT	2 cut(s)	1280, 2142
BsaAI	YACGTR	1 cut(s)	2090
BsaHI	GRCGYC	1 cut(s)	52
BsaJI	CCNNGG	4 cut(s)	745, 1377, 1829, 1863
BsaWI	WCCGGW	1 cut(s)	1693
BsaXI	ACNNNNNCTCC	4 cut(s)	75, 105, 1224, 1254
Bsc4I	CCNNNNNNNGG	8 cut(s)	93, 151, 586, 766, 1255, 1370, 1869, 2176
Bse118I	RCCGGY	1 cut(s)	1967
Bse1I	ACTGG	2 cut(s)	1445, 1981
Bse21I	CCTNAGG	2 cut(s)	585, 1305
Bse3DI	GCAATG	3 cut(s)	232, 693, 705
BseBI	CCWGG	2 cut(s)	285, 1379
BseCI	ATCGAT	2 cut(s)	1280, 2142
BseDI	CCNNGG	4 cut(s)	745, 1377, 1829, 1863
BseGI	GGATG	3 cut(s)	730, 1977, 2070
BseLI	CCNNNNNNNGG	8 cut(s)	93, 151, 586, 766, 1255, 1370, 1869, 2176
BseMI	GCAATG	3 cut(s)	232, 693, 705
BseMII	CTCAG	6 cut(s)	270, 599, 1319, 1373, 1629, 1706
BseNI	ACTGG	2 cut(s)	1445, 1981
BseRI	GAGGAG	4 cut(s)	170, 1245, 1856, 1913
BseSI	GKGCMC	3 cut(s)	1428, 1862, 2241
BseX3I	CGGCCG	1 cut(s)	1969
BseXI	GCAGC	5 cut(s)	902, 905, 1154, 1436, 1636
BseYI	CCCAGC	2 cut(s)	965, 1264
BsgI	GTGCAG	3 cut(s)	237, 239, 2066
Bsh1236I	CGCG	5 cut(s)	111, 212, 341, 1831, 1956
Bsh1285I	CGRYCG	2 cut(s)	216, 1972
BshFI	GGCC	5 cut(s)	17, 579, 1259, 1949, 1971
BshNI	GGYRCC	2 cut(s)	1423, 1859
BshVI	ATCGAT	2 cut(s)	1280, 2142
BsiEI	CGRYCG	2 cut(s)	216, 1972
BsiHKAI	GWGCWC	2 cut(s)	1071, 1905
BsiHKCI	CYCGRG	1 cut(s)	2450
BsiSI	CCGG	5 cut(s)	94, 130, 1694, 1710, 1968
BslFI	GGGAC	1 cut(s)	1387
BslI	CCNNNNNNNGG	8 cut(s)	93, 151, 586, 766, 1255, 1370, 1869, 2176
BsmAI	GTCTC	3 cut(s)	127, 1775, 2329
BsmBI	CGTCTC	1 cut(s)	1775
BsmFI	GGGAC	1 cut(s)	1387
BsnI	GGCC	5 cut(s)	17, 579, 1259, 1949, 1971
BsoBI	CYCGRG	1 cut(s)	2450
Bsp1286I	GDGCHC	5 cut(s)	1071, 1428, 1862, 1905, 2241
Bsp1407I	TGTACA	1 cut(s)	229
Bsp143I	GATC	8 cut(s)	831, 871, 1123, 1564, 1569, 1785, 2139, 2370
Bsp1720I	GCTNAGC	2 cut(s)	279, 1359
Bsp19I	CCATGG	2 cut(s)	745, 1863
BspANI	GGCC	5 cut(s)	17, 579, 1259, 1949, 1971
BspCNI	CTCAG	6 cut(s)	271, 598, 1318, 1372, 1628, 1707
BspDI	ATCGAT	2 cut(s)	1280, 2142
BspFNI	CGCG	5 cut(s)	111, 212, 341, 1831, 1956
BspHI	TCATGA	2 cut(s)	1338, 1572
BspLI	GGNNCC	5 cut(s)	319, 1375, 1376, 1425, 1861
BspMAI	CTGCAG	2 cut(s)	400, 892
BspMI	ACCTGC	2 cut(s)	2123, 2236
BspPI	GGATC	2 cut(s)	1572, 2147
BspQI	GCTCTTC	1 cut(s)	2066
BspT107I	GGYRCC	2 cut(s)	1423, 1859
BspTI	CTTAAG	1 cut(s)	788
BsrDI	GCAATG	3 cut(s)	232, 693, 705
BsrFI	RCCGGY	1 cut(s)	1967
BsrGI	TGTACA	1 cut(s)	229
BsrI	ACTGG	2 cut(s)	1445, 1981
BssAI	RCCGGY	1 cut(s)	1967
BssECI	CCNNGG	4 cut(s)	745, 1377, 1829, 1863
BssMI	GATC	8 cut(s)	831, 871, 1123, 1564, 1569, 1785, 2139, 2370
BssNAI	GTATAC	1 cut(s)	1621
BssNI	GRCGYC	1 cut(s)	52
BssT1I	CCWWGG	2 cut(s)	745, 1863
Bst1107I	GTATAC	1 cut(s)	1621
Bst2UI	CCWGG	2 cut(s)	285, 1379
Bst4CI	ACNGT	9 cut(s)	199, 535, 1046, 1051, 1469, 1478, 1625, 1677, 1747
Bst6I	CTCTTC	2 cut(s)	1202, 2066
BstACI	GRCGYC	1 cut(s)	52
BstAFI	CTTAAG	1 cut(s)	788
BstAPI	GCANNNNNTGC	2 cut(s)	887, 899
BstAUI	TGTACA	1 cut(s)	229
BstBAI	YACGTR	1 cut(s)	2090
BstC8I	GCNNGC	6 cut(s)	222, 577, 963, 1520, 1919, 1969
BstDEI	CTNAG	6 cut(s)	279, 585, 1305, 1359, 1615, 1715
BstDSI	CCRYGG	3 cut(s)	745, 1829, 1863
BstEII	GGTNACC	1 cut(s)	749
BstENI	CCTNNNNNAGG	1 cut(s)	584
BstF5I	GGATG	3 cut(s)	730, 1977, 2070
BstFNI	CGCG	5 cut(s)	111, 212, 341, 1831, 1956
BstHHI	GCGC	3 cut(s)	341, 1020, 1956
BstKTI	GATC	8 cut(s)	834, 874, 1126, 1567, 1572, 1788, 2142, 2373
BstMAI	GTCTC	3 cut(s)	127, 1775, 2329
BstMBI	GATC	8 cut(s)	831, 871, 1123, 1564, 1569, 1785, 2139, 2370
BstMCI	CGRYCG	2 cut(s)	216, 1972
BstMWI	GCNNNNNNNGC	9 cut(s)	23, 640, 887, 899, 1075, 1455, 2007, 2044, 2053
BstNI	CCWGG	2 cut(s)	285, 1379
BstNSI	RCATGY	4 cut(s)	672, 1075, 1559, 1921
BstPI	GGTNACC	1 cut(s)	749
BstSCI	CCNGG	3 cut(s)	283, 1377, 1708
BstSFI	CTRYAG	2 cut(s)	396, 888
BstSLI	GKGCMC	3 cut(s)	1428, 1862, 2241
BstUI	CGCG	5 cut(s)	111, 212, 341, 1831, 1956
BstV1I	GCAGC	5 cut(s)	902, 905, 1154, 1436, 1636
BstV2I	GAAGAC	3 cut(s)	1479, 1746, 2010
BstX2I	RGATCY	1 cut(s)	1564
BstYI	RGATCY	1 cut(s)	1564
BstZ17I	GTATAC	1 cut(s)	1621
BstZI	CGGCCG	1 cut(s)	1969
Bsu15I	ATCGAT	2 cut(s)	1280, 2142
Bsu36I	CCTNAGG	2 cut(s)	585, 1305
BsuI	GTATCC	2 cut(s)	1075, 2173
BsuRI	GGCC	5 cut(s)	17, 579, 1259, 1949, 1971
BsuTUI	ATCGAT	2 cut(s)	1280, 2142
BtgI	CCRYGG	3 cut(s)	745, 1829, 1863
BtgZI	GCGATG	1 cut(s)	1412
BtsCI	GGATG	3 cut(s)	730, 1977, 2070
BtsI	GCAGTG	3 cut(s)	393, 405, 885
BtsIMutI	CAGTG	5 cut(s)	393, 405, 885, 1425, 1752
BveI	ACCTGC	2 cut(s)	2123, 2236
Cac8I	GCNNGC	6 cut(s)	222, 577, 963, 1520, 1919, 1969
CaiI	CAGNNNCTG	1 cut(s)	896
CciI	TCATGA	2 cut(s)	1338, 1572
CfoI	GCGC	3 cut(s)	341, 1020, 1956
Cfr10I	RCCGGY	1 cut(s)	1967
Cfr13I	GGNCC	2 cut(s)	1374, 1947
Cfr42I	CCGCGG	1 cut(s)	1832
ClaI	ATCGAT	2 cut(s)	1280, 2142
CseI	GACGC	1 cut(s)	60
Csp6I	GTAC	6 cut(s)	33, 230, 371, 1047, 1191, 1579
CspCI	CAANNNNNGTGG	2 cut(s)	2116, 2151
CviQI	GTAC	6 cut(s)	33, 230, 371, 1047, 1191, 1579
DdeI	CTNAG	6 cut(s)	279, 585, 1305, 1359, 1615, 1715
DpnI	GATC	8 cut(s)	833, 873, 1125, 1566, 1571, 1787, 2141, 2372
DpnII	GATC	8 cut(s)	831, 871, 1123, 1564, 1569, 1785, 2139, 2370
DraI	TTTAAA	1 cut(s)	1149
EaeI	YGGCCR	1 cut(s)	1969
EagI	CGGCCG	1 cut(s)	1969
Eam1104I	CTCTTC	2 cut(s)	1202, 2066
EarI	CTCTTC	2 cut(s)	1202, 2066
Ecl136II	GAGCTC	1 cut(s)	1903
EclXI	CGGCCG	1 cut(s)	1969
Eco130I	CCWWGG	2 cut(s)	745, 1863
Eco147I	AGGCCT	2 cut(s)	579, 1259
Eco24I	GRGCYC	1 cut(s)	1905
Eco32I	GATATC	2 cut(s)	663, 1215
Eco47I	GGWCC	1 cut(s)	1374
Eco52I	CGGCCG	1 cut(s)	1969
Eco53kI	GAGCTC	1 cut(s)	1903
Eco57I	CTGAAG	4 cut(s)	1856, 1868, 2047, 2286
Eco81I	CCTNAGG	2 cut(s)	585, 1305
Eco88I	CYCGRG	1 cut(s)	2450
Eco91I	GGTNACC	1 cut(s)	749
EcoICRI	GAGCTC	1 cut(s)	1903
EcoNI	CCTNNNNNAGG	1 cut(s)	584
EcoO109I	RGGNCCY	1 cut(s)	1374
EcoO65I	GGTNACC	1 cut(s)	749
EcoRII	CCWGG	2 cut(s)	283, 1377
EcoRV	GATATC	2 cut(s)	663, 1215
EcoT14I	CCWWGG	2 cut(s)	745, 1863
EcoT22I	ATGCAT	1 cut(s)	941
EcoT38I	GRGCYC	1 cut(s)	1905
ErhI	CCWWGG	2 cut(s)	745, 1863
Esp3I	CGTCTC	1 cut(s)	1775
FalI	AAGNNNNNCTT	2 cut(s)	2045, 2077
FaqI	GGGAC	1 cut(s)	1387
FauI	CCCGC	6 cut(s)	86, 116, 134, 954, 1071, 1527
FauNDI	CATATG	1 cut(s)	313
FbaI	TGATCA	2 cut(s)	1123, 1569
FblI	GTMKAC	3 cut(s)	543, 1620, 1720
FokI	GGATG	3 cut(s)	737, 1964, 2057
FriOI	GRGCYC	1 cut(s)	1905
FspBI	CTAG	3 cut(s)	452, 623, 2075
GlaI	GCGC	3 cut(s)	340, 1019, 1955
GsaI	CCCAGC	2 cut(s)	969, 1268
HaeIII	GGCC	5 cut(s)	17, 579, 1259, 1949, 1971
HapII	CCGG	5 cut(s)	94, 130, 1694, 1710, 1968
HgaI	GACGC	1 cut(s)	60
HhaI	GCGC	3 cut(s)	341, 1020, 1956
Hin1I	GRCGYC	1 cut(s)	52
Hin6I	GCGC	3 cut(s)	339, 1018, 1954
HinP1I	GCGC	3 cut(s)	339, 1018, 1954
HincII	GTYRAC	1 cut(s)	1721
HindII	GTYRAC	1 cut(s)	1721
HpaII	CCGG	5 cut(s)	94, 130, 1694, 1710, 1968
Hpy166II	GTNNAC	6 cut(s)	230, 544, 1322, 1621, 1682, 1721
Hpy188I	TCNGA	9 cut(s)	539, 660, 828, 1569, 1702, 1716, 1756, 2157, 2278
Hpy8I	GTNNAC	6 cut(s)	230, 544, 1322, 1621, 1682, 1721
Hpy99I	CGWCG	4 cut(s)	54, 60, 272, 2378
HpyCH4III	ACNGT	9 cut(s)	199, 535, 1046, 1051, 1469, 1478, 1625, 1677, 1747
HpyCH4IV	ACGT	3 cut(s)	619, 1544, 2089
HpyF10VI	GCNNNNNNNGC	9 cut(s)	23, 640, 887, 899, 1075, 1455, 2007, 2044, 2053
HpyF3I	CTNAG	6 cut(s)	279, 585, 1305, 1359, 1615, 1715
HpySE526I	ACGT	3 cut(s)	619, 1544, 2089
Hsp92I	GRCGYC	1 cut(s)	52
HspAI	GCGC	3 cut(s)	339, 1018, 1954
KflI	GGGWCCC	1 cut(s)	1374
KroI	GCCGGC	1 cut(s)	1967
KroNI	GCCGGC	1 cut(s)	1969
Ksp22I	TGATCA	2 cut(s)	1123, 1569
KspI	CCGCGG	1 cut(s)	1832
Kzo9I	GATC	8 cut(s)	831, 871, 1123, 1564, 1569, 1785, 2139, 2370
LguI	GCTCTTC	1 cut(s)	2066
LmnI	GCTCC	3 cut(s)	385, 1843, 1900
Lsp1109I	GCAGC	5 cut(s)	902, 905, 1154, 1436, 1636
LweI	GCATC	6 cut(s)	889, 1332, 1389, 1968, 2401, 2413
MaeI	CTAG	3 cut(s)	452, 623, 2075
MaeII	ACGT	3 cut(s)	619, 1544, 2089
MaeIII	GTNAC	5 cut(s)	749, 769, 1689, 2066, 2098
MalI	GATC	8 cut(s)	833, 873, 1125, 1566, 1571, 1787, 2141, 2372
MboI	GATC	8 cut(s)	831, 871, 1123, 1564, 1569, 1785, 2139, 2370
MfeI	CAATTG	2 cut(s)	1326, 2456
MflI	RGATCY	1 cut(s)	1564
MhlI	GDGCHC	5 cut(s)	1071, 1428, 1862, 1905, 2241
MlyI	GAGTC	5 cut(s)	93, 242, 549, 1297, 1726
MmeI	TCCRAC	1 cut(s)	669
Mph1103I	ATGCAT	1 cut(s)	941
MroNI	GCCGGC	1 cut(s)	1967
MroXI	GAANNNNTTC	1 cut(s)	1805
MseI	TTAA	9 cut(s)	326, 629, 789, 816, 956, 1148, 1368, 1410, 2256
MslI	CAYNNNNRTG	6 cut(s)	200, 398, 498, 698, 2088, 2442
MspA1I	CMGCKG	2 cut(s)	896, 1831
MspCI	CTTAAG	1 cut(s)	788
MspI	CCGG	5 cut(s)	94, 130, 1694, 1710, 1968
MspR9I	CCNGG	3 cut(s)	285, 1379, 1710
MunI	CAATTG	2 cut(s)	1326, 2456
MvaI	CCWGG	2 cut(s)	285, 1379
MvnI	CGCG	5 cut(s)	111, 212, 341, 1831, 1956
MwoI	GCNNNNNNNGC	9 cut(s)	23, 640, 887, 899, 1075, 1455, 2007, 2044, 2053
NaeI	GCCGGC	1 cut(s)	1969
NciI	CCSGG	1 cut(s)	1710
NcoI	CCATGG	2 cut(s)	745, 1863
NdeI	CATATG	1 cut(s)	313
NdeII	GATC	8 cut(s)	831, 871, 1123, 1564, 1569, 1785, 2139, 2370
NgoMIV	GCCGGC	1 cut(s)	1967
NlaIV	GGNNCC	5 cut(s)	319, 1375, 1376, 1425, 1861
NmuCI	GTSAC	4 cut(s)	749, 769, 1689, 2098
NsiI	ATGCAT	1 cut(s)	941
NspI	RCATGY	4 cut(s)	672, 1075, 1559, 1921
OliI	CACNNNNGTG	3 cut(s)	200, 398, 2088
PaeI	GCATGC	1 cut(s)	1921
PagI	TCATGA	2 cut(s)	1338, 1572
PaqCI	CACCTGC	1 cut(s)	2123
PceI	AGGCCT	2 cut(s)	579, 1259
PciI	ACATGT	1 cut(s)	1071
PciSI	GCTCTTC	1 cut(s)	2066
PcsI	WCGNNNNNNNCGW	1 cut(s)	55
PdiI	GCCGGC	1 cut(s)	1969
PdmI	GAANNNNTTC	1 cut(s)	1805
PflFI	GACNNNGTC	1 cut(s)	54
PflMI	CCANNNNNTGG	2 cut(s)	151, 2176
PleI	GAGTC	5 cut(s)	93, 242, 548, 1296, 1725
PpsI	GAGTC	5 cut(s)	93, 242, 548, 1296, 1725
Ppu21I	YACGTR	1 cut(s)	2090
PpuMI	RGGWCCY	1 cut(s)	1374
PscI	ACATGT	1 cut(s)	1071
PshBI	ATTAAT	1 cut(s)	816
Psp124BI	GAGCTC	1 cut(s)	1905
Psp5II	RGGWCCY	1 cut(s)	1374
Psp6I	CCWGG	2 cut(s)	283, 1377
PspEI	GGTNACC	1 cut(s)	749
PspFI	CCCAGC	2 cut(s)	965, 1264
PspGI	CCWGG	2 cut(s)	283, 1377
PspN4I	GGNNCC	5 cut(s)	319, 1375, 1376, 1425, 1861
PspPI	GGNCC	2 cut(s)	1374, 1947
PspPPI	RGGWCCY	1 cut(s)	1374
PstI	CTGCAG	2 cut(s)	400, 892
PstNI	CAGNNNCTG	1 cut(s)	896
PsuI	RGATCY	1 cut(s)	1564
PsyI	GACNNNGTC	1 cut(s)	54
PvuII	CAGCTG	1 cut(s)	896
RsaI	GTAC	6 cut(s)	34, 231, 372, 1048, 1192, 1580
RsaNI	GTAC	6 cut(s)	33, 230, 371, 1047, 1191, 1579
RseI	CAYNNNNRTG	6 cut(s)	200, 398, 498, 698, 2088, 2442
SacI	GAGCTC	1 cut(s)	1905
SacII	CCGCGG	1 cut(s)	1832
SalI	GTCGAC	1 cut(s)	1719
SapI	GCTCTTC	1 cut(s)	2066
SaqAI	TTAA	9 cut(s)	326, 629, 789, 816, 956, 1148, 1368, 1410, 2256
Sau3AI	GATC	8 cut(s)	831, 871, 1123, 1564, 1569, 1785, 2139, 2370
Sau96I	GGNCC	2 cut(s)	1374, 1947
ScaI	AGTACT	1 cut(s)	1192
SchI	GAGTC	5 cut(s)	93, 242, 549, 1297, 1726
ScrFI	CCNGG	3 cut(s)	285, 1379, 1710
SduI	GDGCHC	5 cut(s)	1071, 1428, 1862, 1905, 2241
SfaNI	GCATC	6 cut(s)	889, 1332, 1389, 1968, 2401, 2413
SfcI	CTRYAG	2 cut(s)	396, 888
Sfr303I	CCGCGG	1 cut(s)	1832
SgrBI	CCGCGG	1 cut(s)	1832
SinI	GGWCC	1 cut(s)	1374
SmiMI	CAYNNNNRTG	6 cut(s)	200, 398, 498, 698, 2088, 2442
SmlI	CTYRAG	2 cut(s)	788, 1904
SmoI	CTYRAG	2 cut(s)	788, 1904
SphI	GCATGC	1 cut(s)	1921
SseBI	AGGCCT	2 cut(s)	579, 1259
SspI	AATATT	1 cut(s)	954
SspMI	CTAG	3 cut(s)	452, 623, 2075
SstI	GAGCTC	1 cut(s)	1905
StuI	AGGCCT	2 cut(s)	579, 1259
StyD4I	CCNGG	3 cut(s)	283, 1377, 1708
StyI	CCWWGG	2 cut(s)	745, 1863
TaaI	ACNGT	9 cut(s)	199, 535, 1046, 1051, 1469, 1478, 1625, 1677, 1747
TaiI	ACGT	3 cut(s)	622, 1547, 2092
TatI	WGTACW	5 cut(s)	32, 229, 370, 1190, 1578
Tru1I	TTAA	9 cut(s)	326, 629, 789, 816, 956, 1148, 1368, 1410, 2256
Tru9I	TTAA	9 cut(s)	326, 629, 789, 816, 956, 1148, 1368, 1410, 2256
TscAI	CASTG	5 cut(s)	400, 405, 892, 1425, 1752
TseFI	GTSAC	4 cut(s)	749, 769, 1689, 2098
TseI	GCWGC	5 cut(s)	890, 893, 1142, 1449, 1649
Tsp45I	GTSAC	4 cut(s)	749, 769, 1689, 2098
TspGWI	ACGGA	3 cut(s)	361, 2105, 2198
TspRI	CASTG	5 cut(s)	400, 405, 892, 1425, 1752
Tth111I	GACNNNGTC	1 cut(s)	54
Van91I	CCANNNNNTGG	2 cut(s)	151, 2176
Vha464I	CTTAAG	1 cut(s)	788
VpaK11BI	GGWCC	1 cut(s)	1374
VspI	ATTAAT	1 cut(s)	816
XagI	CCTNNNNNAGG	1 cut(s)	584
XapI	RAATTY	2 cut(s)	737, 979
XceI	RCATGY	4 cut(s)	672, 1075, 1559, 1921
XmiI	GTMKAC	3 cut(s)	543, 1620, 1720
XmnI	GAANNNNTTC	1 cut(s)	1805
XspI	CTAG	3 cut(s)	452, 623, 2075
ZrmI	AGTACT	1 cut(s)	1192
Zsp2I	ATGCAT	1 cut(s)	941

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.