pycom08g18240

Protein of unknown function (DUF1005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013323
Physical Location & Seq
Forward (+)
18496899 .. 18498408
1510 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g18240.1

Sequence Viewer

Length: 1335 bp
ATGGATCCATGCCCATTTGTGCGGGTCACAGTGGGCAATCTGGCTCTCAAGATCCCCGTCGCCTCCAAGCCCGCCAGCTCCGTCGTCCACCCTTCCTCCTCTCCGTGCTTCTGCAAGATCAAGTTCAACAATCTCAACATCCCCCCTCAGAACGCCGTCGTCCCTTGCCTACATCCCGACACCCAAACCCTCGACTACCCCGCCACGGCCGCCACCTTCCACCTCAGCAAGTCTGATCTCGACCGCCTCGCCTCCAAGTCCATCTTCGCCTCCAAGCTCTGCCTCAAAATCTCCATCTACACCGGCCGCAGGGGCACCACCTGCGGCGTCAACTCCGGGAGGCTGTTGGGCCGGGTTTCGGTTCCCTTGGATCTGGCGGGAACCGAGTCCAAGCCCAGCGTTTTCCACAACGGGTGGGTCTCCGTGGGAAAAGGGGCCAACAAGGGCGGGTCCTCGGCTCAGTCCCAGTTCCATTTGAATGTGAAGGCCGAACCCGACCCGAGATTCGTGTTCCAGTTCGACGGTGAACCCGAGTGTAGCCCGCAAGTGTTTCAGATCCAAGGCAACCTCCGGCAGCCAGTCTTCACCTGCACGTTCAGCTTCAGAACACCCGCCGACCGCACTCAGAGATCCAGGTCGTTACAGTCGGACCAAGGCAGCTCTCGGAGCTGGCTAAGCTCGTTCGGGAGCGAGCGAGAGCGACACGGAAGGGAACGAAAGGGCTGGTCGATTACGGTCCACGACCTCTCAGGGTCGCCTGTGGCTGCAGCCTCGATGGTCACGCCCTTCGTGGCCTCGCCAGGGTCGGACCGCGTCAGCCGGTCCAACCCAGGCTCCTGGCTCATTCTCCGGCCCGGCGACAACACCTGGAAGCCCTGGGGACGCTTGGAGGCCTGGCGCGAACGAGGTGGCGCCGACGGCCTCGGTTACCGCTTTGAACTCATTCCCGACACCAGCGGCCCCGGCGTCGTGTTGGCGGAGTCCACTCTCAGCCTAAACAAGGGCGGGAAATTCGTCCTGGATCTCGGGTCAGGGTCCACTAACCGAGCCGGCCCAAATTCTCCGGGTTGTAGCCCGAGGGGGAGCGGGGACTTCGGGTACGGGTTGTGGCCTTTCTGCATGTACAGAGGGTTCGTGATGTCTGCGAAGGTGGAAGGTGAGGGGAGATGCAGCAAACCTGCGGTGGAGGTGAGCGTGCAGCACGTGAACTGCACGGAGGACGCAGCGGCTTTCGTGGCATTGGCCGCAGCCGTTGATCTGAGCATGGATGCCTGCAGACTTTTCTCGCAGCGGCTGAGGAAGGAGCTGTGTCAGCAGTCCGATCTGGTCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1005

Protein Analysis

445

Amino Acids

47.78

Weight (kDa)

9.21

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1005 PF06219 1 - 437 9.2e-207 Protein of unknown function (DUF1005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10020
fragaria_vesca FvH4_5g33420
malus_domestica MD08G1211700.v1.1 MD15G1395200.v1.1
prunus_persica Prupe.1G544000_v2.0.a1
pyrus_communis pycom08g18240 pycom15g35570
rosa_chinensis RchiOBHm_Chr7g0232151
rosa_laevigata RLG00000001387
rosa_multiflora Rmu_sc0000616.1_g000015 Rmu_sc0004780.1_g000008
rosa_roxburghii Rroxscaffold_3G00228740
rosa_rugosa Rorug07G0269800
rosa_samantha Rh7AG424400 Rh7BG398500 Rh7CG444700 Rh7DG416400
rosa_wichuraiana Rw7G035090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 329, 596
Acc36I ACCTGC 3 cut(s) 329, 596, 1186
AccB1I GGYRCC 2 cut(s) 314, 911
AccBSI CCGCTC 1 cut(s) 1086
AccII CGCG 2 cut(s) 813, 900
AclWI GGATC 6 cut(s) 12, 46, 378, 550, 624, 1029
AcoI YGGCCR 3 cut(s) 207, 304, 1242
AcsI RAATTY 2 cut(s) 1010, 1057
AcuI CTGAAG 1 cut(s) 586
AcvI CACGTG 1 cut(s) 1204
AcyI GRCGYC 3 cut(s) 327, 912, 966
AfaI GTAC 2 cut(s) 1100, 1124
AfiI CCNNNNNNNGG 5 cut(s) 205, 309, 358, 801, 1000
AgsI TTSAA 3 cut(s) 127, 478, 938
AjnI CCWGG 8 cut(s) 632, 799, 829, 836, 866, 875, 893, 1017
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 7 cut(s) 78, 277, 600, 660, 669, 678, 1306
AluI AGCT 7 cut(s) 78, 277, 600, 660, 669, 678, 1306
Alw26I GTCTC 1 cut(s) 424
AlwI GGATC 6 cut(s) 12, 46, 378, 550, 624, 1029
AlwNI CAGNNNCTG 1 cut(s) 1294
Ama87I CYCGRG 4 cut(s) 499, 530, 1025, 1075
ApeKI GCWGC 9 cut(s) 574, 657, 764, 767, 1170, 1198, 1223, 1247, 1288
ApoI RAATTY 2 cut(s) 1010, 1057
ArsI GACNNNNNNTTYG 2 cut(s) 488, 520
Asp700I GAANNNNTTC 1 cut(s) 942
AspLEI GCGC 2 cut(s) 900, 914
AsuC2I CCSGG 5 cut(s) 337, 353, 855, 963, 1065
AsuHPI GGTGA 4 cut(s) 536, 577, 1169, 1201
AvaI CYCGRG 4 cut(s) 499, 530, 1025, 1075
AvaII GGWCC 6 cut(s) 450, 649, 736, 808, 822, 1035
BaeGI GKGCMC 1 cut(s) 317
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 314, 911
BbrPI CACGTG 1 cut(s) 1204
BbsI GAAGAC 1 cut(s) 574
BbvCI CCTCAGC 2 cut(s) 224, 1295
BbvI GCAGC 9 cut(s) 586, 669, 751, 779, 1182, 1210, 1235, 1259, 1300
BccI CCATC 3 cut(s) 269, 302, 769
BceAI ACGGC 4 cut(s) 140, 222, 934, 1235
BciT130I CCWGG 8 cut(s) 634, 801, 831, 838, 868, 877, 895, 1019
BcnI CCSGG 5 cut(s) 337, 353, 855, 963, 1065
BcoDI GTCTC 1 cut(s) 424
BfmI CTRYAG 2 cut(s) 765, 1273
BfoI RGCGCY 1 cut(s) 915
BfuAI ACCTGC 3 cut(s) 329, 596, 1186
BglI GCCNNNNNGGC 1 cut(s) 312
BlpI GCTNAGC 1 cut(s) 674
Bme18I GGWCC 6 cut(s) 450, 649, 736, 808, 822, 1035
BmeT110I CYCGRG 4 cut(s) 499, 530, 1025, 1075
BmrI ACTGGG 1 cut(s) 460
BmsI GCATC 2 cut(s) 1157, 1258
BmuI ACTGGG 1 cut(s) 460
BpiI GAAGAC 1 cut(s) 574
Bpu10I CCTNAGC 2 cut(s) 224, 1295
Bpu1102I GCTNAGC 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 32
BpuMI CCSGG 5 cut(s) 337, 353, 855, 963, 1065
BsaAI YACGTR 1 cut(s) 1204
BsaHI GRCGYC 3 cut(s) 327, 912, 966
BsaI GGTCTC 1 cut(s) 424
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 818, 848
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 309, 358, 801, 1000
Bse118I RCCGGY 3 cut(s) 302, 819, 1049
Bse1I ACTGG 3 cut(s) 466, 514, 578
BseBI CCWGG 8 cut(s) 634, 801, 831, 838, 868, 877, 895, 1019
BseGI GGATG 3 cut(s) 138, 172, 1273
BseLI CCNNNNNNNGG 5 cut(s) 205, 309, 358, 801, 1000
BseMII CTCAG 8 cut(s) 161, 238, 473, 638, 762, 1003, 1250, 1286
BseNI ACTGG 3 cut(s) 466, 514, 578
BseRI GAGGAG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 317
BseX3I CGGCCG 2 cut(s) 207, 304
BseXI GCAGC 9 cut(s) 586, 669, 751, 779, 1182, 1210, 1235, 1259, 1300
BseYI CCCAGC 1 cut(s) 395
BsgI GTGCAG 3 cut(s) 574, 1195, 1217
Bsh1236I CGCG 2 cut(s) 813, 900
Bsh1285I CGRYCG 4 cut(s) 210, 244, 307, 619
BshNI GGYRCC 2 cut(s) 314, 911
BsiEI CGRYCG 4 cut(s) 210, 244, 307, 619
BsiHKCI CYCGRG 4 cut(s) 499, 530, 1025, 1075
BslFI GGGAC 4 cut(s) 146, 448, 894, 1103
BslI CCNNNNNNNGG 5 cut(s) 205, 309, 358, 801, 1000
BsmAI GTCTC 1 cut(s) 424
BsmFI GGGAC 4 cut(s) 146, 448, 894, 1103
Bso31I GGTCTC 1 cut(s) 424
BsoBI CYCGRG 4 cut(s) 499, 530, 1025, 1075
Bsp1286I GDGCHC 1 cut(s) 317
Bsp1407I TGTACA 1 cut(s) 1122
Bsp1720I GCTNAGC 1 cut(s) 674
BspCNI CTCAG 8 cut(s) 160, 237, 472, 637, 761, 1002, 1251, 1287
BspFNI CGCG 2 cut(s) 813, 900
BspMAI CTGCAG 2 cut(s) 769, 1277
BspMI ACCTGC 3 cut(s) 329, 596, 1186
BspPI GGATC 6 cut(s) 12, 46, 378, 550, 624, 1029
BspT107I GGYRCC 2 cut(s) 314, 911
BspTNI GGTCTC 1 cut(s) 424
BsrBI CCGCTC 1 cut(s) 1086
BsrFI RCCGGY 3 cut(s) 302, 819, 1049
BsrGI TGTACA 1 cut(s) 1122
BsrI ACTGG 3 cut(s) 466, 514, 578
BssAI RCCGGY 3 cut(s) 302, 819, 1049
BssNI GRCGYC 3 cut(s) 327, 912, 966
BssT1I CCWWGG 3 cut(s) 366, 559, 652
Bst2UI CCWGG 8 cut(s) 634, 801, 831, 838, 868, 877, 895, 1019
Bst4CI ACNGT 4 cut(s) 31, 524, 645, 736
BstACI GRCGYC 3 cut(s) 327, 912, 966
BstAPI GCANNNNNTGC 1 cut(s) 321
BstAUI TGTACA 1 cut(s) 1122
BstBAI YACGTR 1 cut(s) 1204
BstC8I GCNNGC 8 cut(s) 72, 76, 542, 671, 692, 1051, 1196, 1273
BstDEI CTNAG 9 cut(s) 147, 224, 459, 624, 674, 748, 989, 1259, 1295
BstDSI CCRYGG 2 cut(s) 204, 423
BstEII GGTNACC 1 cut(s) 926
BstENI CCTNNNNNAGG 1 cut(s) 998
BstF5I GGATG 3 cut(s) 138, 172, 1273
BstFNI CGCG 2 cut(s) 813, 900
BstH2I RGCGCY 1 cut(s) 915
BstHHI GCGC 2 cut(s) 900, 914
BstMAI GTCTC 1 cut(s) 424
BstMCI CGRYCG 4 cut(s) 210, 244, 307, 619
BstNI CCWGG 8 cut(s) 634, 801, 831, 838, 868, 877, 895, 1019
BstNSI RCATGY 1 cut(s) 1123
BstPI GGTNACC 1 cut(s) 926
BstSFI CTRYAG 2 cut(s) 765, 1273
BstSLI GKGCMC 1 cut(s) 317
BstUI CGCG 2 cut(s) 813, 900
BstV1I GCAGC 9 cut(s) 586, 669, 751, 779, 1182, 1210, 1235, 1259, 1300
BstV2I GAAGAC 1 cut(s) 574
BstX2I RGATCY 6 cut(s) 4, 51, 370, 555, 629, 1021
BstXI CCANNNNNNTGG 1 cut(s) 837
BstYI RGATCY 6 cut(s) 4, 51, 370, 555, 629, 1021
BstZI CGGCCG 2 cut(s) 207, 304
BtgI CCRYGG 2 cut(s) 204, 423
BtsCI GGATG 3 cut(s) 138, 172, 1273
BtsIMutI CAGTG 1 cut(s) 36
BveI ACCTGC 3 cut(s) 329, 596, 1186
Cac8I GCNNGC 8 cut(s) 72, 76, 542, 671, 692, 1051, 1196, 1273
CaiI CAGNNNCTG 1 cut(s) 1294
CfoI GCGC 2 cut(s) 900, 914
Cfr10I RCCGGY 3 cut(s) 302, 819, 1049
CpoI CGGWCCG 1 cut(s) 808
CseI GACGC 5 cut(s) 316, 802, 891, 955, 1229
Csp6I GTAC 2 cut(s) 1099, 1123
CspI CGGWCCG 1 cut(s) 808
CviAII CATG 3 cut(s) 9, 1120, 1264
CviQI GTAC 2 cut(s) 1099, 1123
DdeI CTNAG 9 cut(s) 147, 224, 459, 624, 674, 748, 989, 1259, 1295
DinI GGCGCC 1 cut(s) 913
EaeI YGGCCR 3 cut(s) 207, 304, 1242
EagI CGGCCG 2 cut(s) 207, 304
EciI GGCGGA 1 cut(s) 992
EclXI CGGCCG 2 cut(s) 207, 304
Eco130I CCWWGG 3 cut(s) 366, 559, 652
Eco147I AGGCCT 1 cut(s) 893
Eco31I GGTCTC 1 cut(s) 424
Eco47I GGWCC 6 cut(s) 450, 649, 736, 808, 822, 1035
Eco52I CGGCCG 2 cut(s) 207, 304
Eco57I CTGAAG 1 cut(s) 586
Eco72I CACGTG 1 cut(s) 1204
Eco88I CYCGRG 4 cut(s) 499, 530, 1025, 1075
Eco91I GGTNACC 1 cut(s) 926
EcoNI CCTNNNNNAGG 1 cut(s) 998
EcoO109I RGGNCCY 1 cut(s) 450
EcoO65I GGTNACC 1 cut(s) 926
EcoRII CCWGG 8 cut(s) 632, 799, 829, 836, 866, 875, 893, 1017
EcoT14I CCWWGG 3 cut(s) 366, 559, 652
EgeI GGCGCC 1 cut(s) 913
EheI GGCGCC 1 cut(s) 913
ErhI CCWWGG 3 cut(s) 366, 559, 652
FaeI CATG 3 cut(s) 12, 1123, 1267
FaiI YATR 3 cut(s) 10, 1121, 1265
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FaqI GGGAC 4 cut(s) 146, 448, 894, 1103
FatI CATG 3 cut(s) 8, 1119, 1263
FauI CCCGC 9 cut(s) 15, 79, 208, 370, 440, 549, 619, 998, 1079
FokI GGATG 3 cut(s) 125, 159, 1280
GlaI GCGC 2 cut(s) 899, 913
GsaI CCCAGC 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 915
HgaI GACGC 5 cut(s) 316, 802, 891, 955, 1229
HhaI GCGC 2 cut(s) 900, 914
Hin1I GRCGYC 3 cut(s) 327, 912, 966
Hin1II CATG 3 cut(s) 12, 1123, 1267
Hin6I GCGC 2 cut(s) 898, 912
HinP1I GCGC 2 cut(s) 898, 912
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 3 cut(s) 386, 504, 980
HphI GGTGA 4 cut(s) 536, 577, 1169, 1201
Hpy166II GTNNAC 7 cut(s) 88, 331, 527, 739, 984, 1038, 1207
Hpy188III TCNNGA 6 cut(s) 49, 176, 239, 685, 947, 1135
Hpy8I GTNNAC 7 cut(s) 88, 331, 527, 739, 984, 1038, 1207
Hpy99I CGWCG 6 cut(s) 62, 86, 161, 524, 920, 971
HpyAV CCTTC 8 cut(s) 102, 226, 478, 702, 796, 1141, 1148, 1294
HpyCH4III ACNGT 4 cut(s) 31, 524, 645, 736
HpyCH4IV ACGT 2 cut(s) 593, 1203
HpyCH4V TGCA 8 cut(s) 114, 591, 767, 1119, 1170, 1198, 1212, 1275
HpyF3I CTNAG 9 cut(s) 147, 224, 459, 624, 674, 748, 989, 1259, 1295
HpySE526I ACGT 2 cut(s) 593, 1203
Hsp92I GRCGYC 3 cut(s) 327, 912, 966
Hsp92II CATG 3 cut(s) 12, 1123, 1267
HspAI GCGC 2 cut(s) 898, 912
KasI GGCGCC 1 cut(s) 911
KroI GCCGGC 1 cut(s) 1049
KroNI GCCGGC 1 cut(s) 1051
LmnI GCTCC 6 cut(s) 83, 666, 687, 839, 1083, 1303
Lsp1109I GCAGC 9 cut(s) 586, 669, 751, 779, 1182, 1210, 1235, 1259, 1300
LweI GCATC 2 cut(s) 1157, 1258
MaeII ACGT 2 cut(s) 593, 1203
MaeIII GTNAC 4 cut(s) 25, 639, 778, 926
MbiI CCGCTC 1 cut(s) 1086
MboII GAAGA 2 cut(s) 256, 574
MflI RGATCY 6 cut(s) 4, 51, 370, 555, 629, 1021
MhlI GDGCHC 1 cut(s) 317
MluCI AATT 2 cut(s) 1010, 1057
Mly113I GGCGCC 1 cut(s) 912
MlyI GAGTC 2 cut(s) 395, 989
MmeI TCCRAC 3 cut(s) 627, 786, 849
MroNI GCCGGC 1 cut(s) 1049
MroXI GAANNNNTTC 1 cut(s) 942
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 3 cut(s) 957, 1226, 1291
MvaI CCWGG 8 cut(s) 634, 801, 831, 838, 868, 877, 895, 1019
MvnI CGCG 2 cut(s) 813, 900
NaeI GCCGGC 1 cut(s) 1051
NarI GGCGCC 1 cut(s) 912
NciI CCSGG 5 cut(s) 337, 353, 855, 963, 1065
NgoMIV GCCGGC 1 cut(s) 1049
NlaIII CATG 3 cut(s) 12, 1123, 1267
NmeAIII GCCGAG 1 cut(s) 434
NmuCI GTSAC 2 cut(s) 25, 778
NspI RCATGY 1 cut(s) 1123
PaqCI CACCTGC 2 cut(s) 329, 596
PasI CCCWGGG 1 cut(s) 876
PceI AGGCCT 1 cut(s) 893
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PdiI GCCGGC 1 cut(s) 1051
PdmI GAANNNNTTC 1 cut(s) 942
PfeI GAWTC 1 cut(s) 504
PflFI GACNNNGTC 1 cut(s) 812
PfoI TCCNGGA 2 cut(s) 335, 1017
PleI GAGTC 2 cut(s) 394, 988
PluTI GGCGCC 1 cut(s) 915
PmaCI CACGTG 1 cut(s) 1204
PmlI CACGTG 1 cut(s) 1204
PpsI GAGTC 2 cut(s) 394, 988
Ppu21I YACGTR 1 cut(s) 1204
PpuMI RGGWCCY 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 8 cut(s) 632, 799, 829, 836, 866, 875, 893, 1017
PspCI CACGTG 1 cut(s) 1204
PspEI GGTNACC 1 cut(s) 926
PspFI CCCAGC 1 cut(s) 395
PspGI CCWGG 8 cut(s) 632, 799, 829, 836, 866, 875, 893, 1017
PspPPI RGGWCCY 1 cut(s) 450
PsrI GAACNNNNNNTAC 2 cut(s) 1115, 1147
PstI CTGCAG 2 cut(s) 769, 1277
PstNI CAGNNNCTG 1 cut(s) 1294
PsuI RGATCY 6 cut(s) 4, 51, 370, 555, 629, 1021
PsyI GACNNNGTC 1 cut(s) 812
RsaI GTAC 2 cut(s) 1100, 1124
RsaNI GTAC 2 cut(s) 1099, 1123
RseI CAYNNNNRTG 1 cut(s) 477
Rsr2I CGGWCCG 1 cut(s) 808
RsrII CGGWCCG 1 cut(s) 808
SchI GAGTC 2 cut(s) 395, 989
SduI GDGCHC 1 cut(s) 317
SfaNI GCATC 2 cut(s) 1157, 1258
SfcI CTRYAG 2 cut(s) 765, 1273
SfoI GGCGCC 1 cut(s) 913
SinI GGWCC 6 cut(s) 450, 649, 736, 808, 822, 1035
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 2 cut(s) 1010, 1057
SseBI AGGCCT 1 cut(s) 893
SspDI GGCGCC 1 cut(s) 911
StuI AGGCCT 1 cut(s) 893
StyI CCWWGG 3 cut(s) 366, 559, 652
TaaI ACNGT 4 cut(s) 31, 524, 645, 736
TaiI ACGT 2 cut(s) 596, 1206
TaqI TCGA 5 cut(s) 192, 240, 519, 728, 773
TasI AATT 2 cut(s) 1010, 1057
TatI WGTACW 1 cut(s) 1122
TauI GCSGC 7 cut(s) 212, 309, 327, 960, 1229, 1247, 1294
TfiI GAWTC 1 cut(s) 504
TscAI CASTG 1 cut(s) 36
TseFI GTSAC 2 cut(s) 25, 778
TseI GCWGC 9 cut(s) 574, 657, 764, 767, 1170, 1198, 1223, 1247, 1288
Tsp45I GTSAC 2 cut(s) 25, 778
TspGWI ACGGA 5 cut(s) 70, 93, 412, 720, 1229
TspRI CASTG 1 cut(s) 36
Tth111I GACNNNGTC 1 cut(s) 812
VpaK11BI GGWCC 6 cut(s) 450, 649, 736, 808, 822, 1035
XagI CCTNNNNNAGG 1 cut(s) 998
XapI RAATTY 2 cut(s) 1010, 1057
XceI RCATGY 1 cut(s) 1123
XmnI GAANNNNTTC 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.