pycom09g12340

LETM1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0008152
Physical Location & Seq
Forward (+)
10849721 .. 10852020
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g12340.1

Sequence Viewer

Length: 852 bp
ATGGCTTTCCGTTTGTCTCCCCTCGAACCTCACCTCTTTTACCATTCAAGAAGATCTGTAAGATCAGAAAACCGACAAAGGGCGATTGTGAATGTGCATAAGCCTTCAAGGTGCAGCACAAATGAACAAGGAATCTATGGCTTTTCGACGAAAAATCACTTGCTCGATTTATTGGTTAGGTGTTACAATTCGTCGAGGTGGTCTTCATCTTGGACCTCATCATCGACAGCGTGTACAGCTTATTCTGGTCAAACAACTTGTGTGAATGGTGCCTCAGTGGCTAGAACTCGTAATCATGAAAACAAATTTAATCGGGTCAATTGCCTTGAATGGGTATTGCATGAATCGGCTAGGAGCTTCTCTGTTGCAGTTGAGTCACTTGAATTGCCAGGAAGTGGTCCAAAGCTTGCAATGGCATGGTCTGGGAAGGATGTCCATCAATGGCATAAACGGATTTCTTATCAGGTTGCAGTTTACGCTTTGTTTAAAACAGCAATTGAAGTAGAAATTTTGCTTTCCAGTGAACGTCACAGCAGCGACTCTTCTCCAGTTAGTGACATCTTAACCCCAAAGATTGATTTGATTGGCGAATACATTGAGAGTCAAATGAAAATGGGGCACTCAGAATTAGTAGAATGGTTTAGAGTGGTGGAACTGCCCCATATGGCAGGATTTTTCATTCCCTTGTTGAGGAAGTGGTCTATGGAATATGCAGGAAGTGGTGTTGCAGGGATGGTTGTGGCTATAAGCTGCTGTGCTGCAGTGGCTAAATTGGGCAACGGTCGTGTTAAGTGCCCTTATTTTTCATTTTCAATTGAAGATGTAATGGTAGAGCTCATGGATCGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LETM1

Protein Analysis

284

Amino Acids

31.81

Weight (kDa)

8.75

Isoelectric Point (pI)

57.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 395
AclWI GGATC 1 cut(s) 849
AcsI RAATTY 2 cut(s) 305, 507
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 4 cut(s) 79, 331, 395, 690
AgsI TTSAA 7 cut(s) 48, 108, 329, 383, 500, 813, 818
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 5 cut(s) 239, 357, 406, 750, 835
AluI AGCT 5 cut(s) 239, 357, 406, 750, 835
Alw21I GWGCWC 1 cut(s) 837
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 1 cut(s) 849
ApeKI GCWGC 4 cut(s) 114, 534, 750, 758
ApoI RAATTY 2 cut(s) 305, 507
AspS9I GGNCC 2 cut(s) 213, 398
AsuHPI GGTGA 1 cut(s) 23
AvaII GGWCC 2 cut(s) 213, 398
BaeGI GKGCMC 2 cut(s) 621, 797
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 837
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 837
BbvI GCAGC 4 cut(s) 126, 546, 737, 745
BccI CCATC 2 cut(s) 444, 727
BciT130I CCWGG 1 cut(s) 390
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 2 cut(s) 282, 351
BfmI CTRYAG 1 cut(s) 759
BglI GCCNNNNNGGC 1 cut(s) 278
BglII AGATCT 1 cut(s) 53
BisI GCNGC 4 cut(s) 115, 535, 751, 759
BlsI GCNGC 4 cut(s) 116, 536, 752, 760
Bme1390I CCNGG 1 cut(s) 390
Bme18I GGWCC 2 cut(s) 213, 398
BmgT120I GGNCC 2 cut(s) 213, 398
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 195
BpmI CTGGAG 1 cut(s) 531
Bsa29I ATCGAT 1 cut(s) 844
BsaBI GATNNNNATC 1 cut(s) 435
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 331, 395, 690
Bse1I ACTGG 2 cut(s) 519, 548
Bse3DI GCAATG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 1 cut(s) 390
BseCI ATCGAT 1 cut(s) 844
BseGI GGATG 2 cut(s) 436, 738
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 4 cut(s) 79, 331, 395, 690
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 2 cut(s) 288, 636
BseNI ACTGG 2 cut(s) 519, 548
BseSI GKGCMC 2 cut(s) 621, 797
BseXI GCAGC 4 cut(s) 126, 546, 737, 745
BsgI GTGCAG 1 cut(s) 133
Bsh1285I CGRYCG 1 cut(s) 784
BshNI GGYRCC 1 cut(s) 269
BshVI ATCGAT 1 cut(s) 844
BsiEI CGRYCG 1 cut(s) 784
BsiHKAI GWGCWC 1 cut(s) 837
BslI CCNNNNNNNGG 4 cut(s) 79, 331, 395, 690
BsmAI GTCTC 1 cut(s) 21
Bsp1286I GDGCHC 3 cut(s) 621, 797, 837
Bsp1407I TGTACA 1 cut(s) 233
Bsp143I GATC 4 cut(s) 53, 62, 841, 845
BspCNI CTCAG 2 cut(s) 287, 635
BspDI ATCGAT 1 cut(s) 844
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 763
BspPI GGATC 1 cut(s) 849
BspT107I GGYRCC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 417
BsrGI TGTACA 1 cut(s) 233
BsrI ACTGG 2 cut(s) 519, 548
BssMI GATC 4 cut(s) 53, 62, 841, 845
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 1 cut(s) 782
Bst6I CTCTTC 1 cut(s) 547
BstAUI TGTACA 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 2 cut(s) 274, 622
BstENI CCTNNNNNAGG 1 cut(s) 688
BstF5I GGATG 2 cut(s) 436, 738
BstKTI GATC 4 cut(s) 56, 65, 844, 848
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 4 cut(s) 53, 62, 841, 845
BstMCI CGRYCG 1 cut(s) 784
BstMWI GCNNNNNNNGC 4 cut(s) 236, 278, 476, 764
BstNI CCWGG 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 388
BstSFI CTRYAG 1 cut(s) 759
BstSLI GKGCMC 2 cut(s) 621, 797
BstV1I GCAGC 4 cut(s) 126, 546, 737, 745
BstV2I GAAGAC 1 cut(s) 195
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
Bsu15I ATCGAT 1 cut(s) 844
BsuTUI ATCGAT 1 cut(s) 844
BtsCI GGATG 2 cut(s) 436, 738
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 3 cut(s) 282, 526, 768
Cac8I GCNNGC 1 cut(s) 408
CciI TCATGA 1 cut(s) 295
Cfr13I GGNCC 2 cut(s) 213, 398
ClaI ATCGAT 1 cut(s) 844
Csp6I GTAC 1 cut(s) 234
CviAII CATG 4 cut(s) 296, 341, 417, 838
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 2 cut(s) 274, 622
DpnI GATC 4 cut(s) 55, 64, 843, 847
DpnII GATC 4 cut(s) 53, 62, 841, 845
DraI TTTAAA 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 547
EarI CTCTTC 1 cut(s) 547
Ecl136II GAGCTC 1 cut(s) 835
Eco24I GRGCYC 1 cut(s) 837
Eco47I GGWCC 2 cut(s) 213, 398
Eco53kI GAGCTC 1 cut(s) 835
EcoICRI GAGCTC 1 cut(s) 835
EcoNI CCTNNNNNAGG 1 cut(s) 688
EcoRII CCWGG 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 837
FaeI CATG 4 cut(s) 299, 344, 420, 841
FatI CATG 4 cut(s) 295, 340, 416, 837
FauNDI CATATG 1 cut(s) 663
Fnu4HI GCNGC 4 cut(s) 115, 535, 751, 759
FokI GGATG 2 cut(s) 443, 745
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 4 cut(s) 115, 535, 751, 759
FspBI CTAG 2 cut(s) 282, 351
GluI GCNGC 4 cut(s) 115, 535, 751, 759
GsuI CTGGAG 1 cut(s) 531
Hin1II CATG 4 cut(s) 299, 344, 420, 841
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 5 cut(s) 132, 344, 374, 539, 601
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 3 cut(s) 234, 475, 524
Hpy188I TCNGA 2 cut(s) 67, 625
Hpy188III TCNNGA 3 cut(s) 48, 296, 849
Hpy8I GTNNAC 3 cut(s) 234, 475, 524
Hpy99I CGWCG 2 cut(s) 151, 196
HpyAV CCTTC 2 cut(s) 114, 421
HpyCH4III ACNGT 1 cut(s) 782
HpyCH4IV ACGT 1 cut(s) 526
HpyCH4V TGCA 9 cut(s) 97, 114, 340, 368, 410, 470, 713, 728, 761
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 278, 476, 764
HpyF3I CTNAG 2 cut(s) 274, 622
HpySE526I ACGT 1 cut(s) 526
Hsp92II CATG 4 cut(s) 299, 344, 420, 841
Kzo9I GATC 4 cut(s) 53, 62, 841, 845
LmnI GCTCC 1 cut(s) 354
Lsp1109I GCAGC 4 cut(s) 126, 546, 737, 745
MaeI CTAG 2 cut(s) 282, 351
MaeII ACGT 1 cut(s) 526
MaeIII GTNAC 4 cut(s) 182, 375, 527, 554
MalI GATC 4 cut(s) 55, 64, 843, 847
MboI GATC 4 cut(s) 53, 62, 841, 845
MboII GAAGA 4 cut(s) 63, 195, 534, 830
MfeI CAATTG 3 cut(s) 319, 495, 813
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 3 cut(s) 621, 797, 837
MluCI AATT 9 cut(s) 187, 305, 319, 383, 495, 507, 626, 770, 813
MlyI GAGTC 3 cut(s) 383, 533, 610
MnlI CCTC 7 cut(s) 32, 39, 44, 189, 226, 283, 684
MseI TTAA 4 cut(s) 309, 486, 563, 789
MspR9I CCNGG 1 cut(s) 390
MunI CAATTG 3 cut(s) 319, 495, 813
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 4 cut(s) 236, 278, 476, 764
NdeI CATATG 1 cut(s) 663
NdeII GATC 4 cut(s) 53, 62, 841, 845
NlaIII CATG 4 cut(s) 299, 344, 420, 841
NlaIV GGNNCC 1 cut(s) 271
NmuCI GTSAC 3 cut(s) 375, 527, 554
PagI TCATGA 1 cut(s) 295
PfeI GAWTC 2 cut(s) 132, 344
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 4 cut(s) 116, 536, 752, 760
PleI GAGTC 3 cut(s) 382, 533, 609
PpsI GAGTC 3 cut(s) 382, 533, 609
Psp124BI GAGCTC 1 cut(s) 837
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 2 cut(s) 213, 398
PstI CTGCAG 1 cut(s) 763
PsuI RGATCY 1 cut(s) 53
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SacI GAGCTC 1 cut(s) 837
SaqAI TTAA 4 cut(s) 309, 486, 563, 789
SatI GCNGC 4 cut(s) 115, 535, 751, 759
Sau3AI GATC 4 cut(s) 53, 62, 841, 845
Sau96I GGNCC 2 cut(s) 213, 398
SchI GAGTC 3 cut(s) 383, 533, 610
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 3 cut(s) 621, 797, 837
SfcI CTRYAG 1 cut(s) 759
SinI GGWCC 2 cut(s) 213, 398
Sse9I AATT 9 cut(s) 187, 305, 319, 383, 495, 507, 626, 770, 813
SspMI CTAG 2 cut(s) 282, 351
SstI GAGCTC 1 cut(s) 837
StyD4I CCNGG 1 cut(s) 388
TaaI ACNGT 1 cut(s) 782
TaiI ACGT 1 cut(s) 529
TaqI TCGA 6 cut(s) 24, 146, 165, 194, 224, 844
TasI AATT 9 cut(s) 187, 305, 319, 383, 495, 507, 626, 770, 813
TatI WGTACW 1 cut(s) 233
TfiI GAWTC 2 cut(s) 132, 344
Tru1I TTAA 4 cut(s) 309, 486, 563, 789
Tru9I TTAA 4 cut(s) 309, 486, 563, 789
TscAI CASTG 3 cut(s) 282, 526, 768
TseFI GTSAC 3 cut(s) 375, 527, 554
TseI GCWGC 4 cut(s) 114, 534, 750, 758
Tsp45I GTSAC 3 cut(s) 375, 527, 554
TspDTI ATGAA 7 cut(s) 138, 195, 312, 357, 623, 667, 795
TspGWI ACGGA 1 cut(s) 466
TspRI CASTG 3 cut(s) 282, 526, 768
Van91I CCANNNNNTGG 1 cut(s) 395
VpaK11BI GGWCC 2 cut(s) 213, 398
XagI CCTNNNNNAGG 1 cut(s) 688
XapI RAATTY 2 cut(s) 305, 507
XspI CTAG 2 cut(s) 282, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.