Rorug02G0358700

LETM1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008152
Physical Location & Seq
Forward (+)
45308717 .. 45310895
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0358700.1

Sequence Viewer

Length: 465 bp
ATGGCTGCTGCTGCTGCTGGTGTGATTGGAGGAAATGGGTCTTCCTGGATTAACCAGTTTAAGGATAAGTCATCGAGGAAGAAGAAGACGGTGAACAGGGCTATGGTTTCCTGTGTTTCTTCTTCTGTGATGGATCCGTATCAGACTTTGAGGATCCGACCCGGTGCCTCTGAATCTGAGGTCAGGAAGGCTTTTAGACAGCTTGCCTTGCAGTATCATCCAGACGTATGTAGAGGAAATAATTGTGGAGTTCAGTTTCACGAAATCAATGAAGCTTATGATATTGTCATGACCAATTTGAGAGAAGAAACGACTCCATTACAGATGTATGAGACTTATGATGAAGGAGAGGATGATTCGATGAGAGGAATGAATGATCCAGATTATGATATGTGGGAGGAATGGATGGGATGGGAAGGAGCTGGTATTAGGGACTACACATCTCATATCAATCCTTACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LETM1

Protein Analysis

154

Amino Acids

17.52

Weight (kDa)

4.94

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 45 - 97 4.6e-15 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AclWI GGATC 5 cut(s) 128, 141, 148, 161, 371
AfiI CCNNNNNNNGG 1 cut(s) 61
AjnI CCWGG 1 cut(s) 44
AluBI AGCT 3 cut(s) 202, 275, 422
AluI AGCT 3 cut(s) 202, 275, 422
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 5 cut(s) 128, 141, 148, 161, 371
ApeKI GCWGC 4 cut(s) 5, 8, 11, 14
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 103
BamHI GGATCC 2 cut(s) 133, 153
BanI GGYRCC 1 cut(s) 164
BbsI GAAGAC 2 cut(s) 33, 92
BccI CCATC 3 cut(s) 124, 400, 405
BciT130I CCWGG 1 cut(s) 46
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 326
BisI GCNGC 4 cut(s) 6, 9, 12, 15
BlsI GCNGC 4 cut(s) 7, 10, 13, 16
Bme1390I CCNGG 2 cut(s) 46, 162
BmiI GGNNCC 3 cut(s) 135, 155, 166
BmrFI CCNGG 2 cut(s) 46, 162
BpiI GAAGAC 2 cut(s) 33, 92
BpuMI CCSGG 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 138
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 55
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 1 cut(s) 46
BseGI GGATG 4 cut(s) 217, 358, 411, 416
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 326
BsmFI GGGAC 1 cut(s) 446
Bsp143I GATC 3 cut(s) 133, 153, 376
BspCNI CTCAG 1 cut(s) 169
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 3 cut(s) 135, 155, 166
BspPI GGATC 5 cut(s) 128, 141, 148, 161, 371
BspT107I GGYRCC 1 cut(s) 164
BsrI ACTGG 1 cut(s) 55
BssMI GATC 3 cut(s) 133, 153, 376
Bst2UI CCWGG 1 cut(s) 46
Bst4CI ACNGT 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 177
BstF5I GGATG 4 cut(s) 217, 358, 411, 416
BstKTI GATC 3 cut(s) 136, 156, 379
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 3 cut(s) 133, 153, 376
BstMWI GCNNNNNNNGC 3 cut(s) 11, 14, 208
BstNI CCWGG 1 cut(s) 46
BstSCI CCNGG 2 cut(s) 44, 160
BstV2I GAAGAC 2 cut(s) 33, 92
BstX2I RGATCY 2 cut(s) 133, 153
BstYI RGATCY 2 cut(s) 133, 153
BtsCI GGATG 4 cut(s) 217, 358, 411, 416
Cac8I GCNNGC 1 cut(s) 204
CciI TCATGA 1 cut(s) 288
CviAII CATG 1 cut(s) 289
CviJI RGCY 6 cut(s) 5, 101, 191, 202, 275, 422
CviKI_1 RGCY 6 cut(s) 5, 101, 191, 202, 275, 422
DdeI CTNAG 1 cut(s) 177
DpnI GATC 3 cut(s) 135, 155, 378
DpnII GATC 3 cut(s) 133, 153, 376
EcoRII CCWGG 1 cut(s) 44
FaeI CATG 1 cut(s) 292
FaiI YATR 9 cut(s) 104, 229, 279, 290, 330, 339, 387, 392, 447
FaqI GGGAC 1 cut(s) 446
FatI CATG 1 cut(s) 288
Fnu4HI GCNGC 4 cut(s) 6, 9, 12, 15
FokI GGATG 4 cut(s) 204, 365, 418, 423
Fsp4HI GCNGC 4 cut(s) 6, 9, 12, 15
GluI GCNGC 4 cut(s) 6, 9, 12, 15
HapII CCGG 1 cut(s) 162
Hin1II CATG 1 cut(s) 292
HindIII AAGCTT 1 cut(s) 273
HinfI GANTC 3 cut(s) 173, 313, 356
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 4 cut(s) 144, 158, 172, 178
Hpy188III TCNNGA 5 cut(s) 184, 221, 260, 289, 380
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 3 cut(s) 181, 338, 410
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 211
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 14, 208
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 1 cut(s) 292
Kzo9I GATC 3 cut(s) 133, 153, 376
LmnI GCTCC 1 cut(s) 419
MaeII ACGT 1 cut(s) 225
MalI GATC 3 cut(s) 135, 155, 378
MboI GATC 3 cut(s) 133, 153, 376
MboII GAAGA 7 cut(s) 33, 91, 94, 97, 111, 114, 317
MflI RGATCY 2 cut(s) 133, 153
MluCI AATT 2 cut(s) 241, 295
MlyI GAGTC 1 cut(s) 307
MmeI TCCRAC 1 cut(s) 181
MnlI CCTC 9 cut(s) 23, 69, 144, 172, 178, 227, 343, 359, 391
MseI TTAA 3 cut(s) 51, 60, 463
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 2 cut(s) 46, 162
MvaI CCWGG 1 cut(s) 46
MwoI GCNNNNNNNGC 3 cut(s) 11, 14, 208
NciI CCSGG 1 cut(s) 162
NdeII GATC 3 cut(s) 133, 153, 376
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 3 cut(s) 135, 155, 166
PagI TCATGA 1 cut(s) 288
PfeI GAWTC 2 cut(s) 173, 356
PfoI TCCNGGA 1 cut(s) 44
PkrI GCNGC 4 cut(s) 7, 10, 13, 16
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 3 cut(s) 135, 155, 166
PsuI RGATCY 2 cut(s) 133, 153
SaqAI TTAA 3 cut(s) 51, 60, 463
SatI GCNGC 4 cut(s) 6, 9, 12, 15
Sau3AI GATC 3 cut(s) 133, 153, 376
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 2 cut(s) 46, 162
SetI ASST 5 cut(s) 183, 204, 228, 277, 424
Sse9I AATT 2 cut(s) 241, 295
StyD4I CCNGG 2 cut(s) 44, 160
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 1 cut(s) 228
TaqI TCGA 2 cut(s) 74, 359
TasI AATT 2 cut(s) 241, 295
TfiI GAWTC 2 cut(s) 173, 356
Tru1I TTAA 3 cut(s) 51, 60, 463
Tru9I TTAA 3 cut(s) 51, 60, 463
TseI GCWGC 4 cut(s) 5, 8, 11, 14
TspDTI ATGAA 3 cut(s) 285, 357, 386
TspGWI ACGGA 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.