Rh2DG428200

LETM1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008152
Physical Location & Seq
Reverse (-)
61958937 .. 61963543
4607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG428200.1

Sequence Viewer

Length: 2163 bp
ATGGCTTCCCATTTGTCTCTGCTCAAACCTCATCTCTCTTACCACTCATCCGTTACACCAGAAAACAGACAAACTATAACCAATTTGCATCAACCTACAAGGTGCAGTACAAAGGAACAAGGAACTTATGGCTTTCCAAAGAAAAGTCTCTTGTGTAGCCTATTGTCTAGATGTTACCCTTCGTCATGGTGGTCTTCATCTAGAACATTATCATCTACAGTTTGTAAAGCTTATTCTGGTGAAGCAAGTAGTGAGAATGGTGCTGCAGTGGCTAAAATCCATGACCGTGAATTCAAATTTAACCGGGTCAATTGCCTTGTGTGGGTGCTGCATGAATCTGCTAGGAGCTTCTCCCTTGCAATTGAGGCGCTAGAATTGATAGGAAGTAGTCCTGGGGTTGCAATGTCATGGTCTGGGAAGGATATACATGCATGGCATAGACGGCTTTCTTATCAGGTGGCACTTTATGCGTTGTTGAAAACAGCGATTGAAGTAGAAACTTTGCTTTCTAGTGAACGTCGTTACAGTGACTCTTCTCTAGTTAGCGATATCTTAACCACAAAGATCAATTCAGTTAGTGAACACATTGAGAGTCAATTGAACATGAGGCACTCAGAGTTAGTAGAATGGTTTAGAGTGGTGGAGTTTCCACGGATTGCAGGATTTTTCATTCCCTTGTTAAGAAAGTGGTCTATGGAGTACGCAGGAAGTGGTGTAGCAGGGATAGTTGTGGCCATCAGCTGCTGCGCTGCAGTGGCTAAATTGGGTCCTGGGCGTGTAAAGTGTTCTTTTTTCGCATTTTGTATTGAGGATGTTATGGTAGAGCTCATGGATCTCTCACACAGTCTTGTATCGGTGGAAAGATTACATCACTTGGCCATTGAGGCAGGATTTGAATTGGACTTTCTGTCCCATTTTGGTAGAAAGGTTCTGCTAAGCAACAAAGGGGAAGAGGTAGAATTTTGGATTGGGTTGGCTTATGAAAAACTTTCATCAGCATTTCACAATGAAAGTGCGATTCCAGGCAAGCAGAATTTTCATGACAAGGTTCAAGCAGATACTTTGGCTACTTTGGGACTGTTTGCATATCTTGGAAGGAAGACTAGATTATTCTTGTCAACAATGGGCATAAACGATATTGATGATCTACTTAAGGACTTCCTTAGCTACTTGGAGTGTGGTAGCATTTTCATTTACCCAGAGTTTGCTTCCATAGCAGTGTATCAACAGTTCATGGAGGTAGTAACTGATGAAATTGGATGGCTTGATTTTTACGCTGCATGTCCTCCAAAAAGTATTCAAGAGAGACGAAAAAGCAAATTGCATACGATTCAGGCGGAAAAAGAGATTGCTCTTTCTGCTGTATTTACTGTATGCTATGATGTCTACTCTGGATTTGCTCACTTTTATCGTTCAACTCAACTGTCCCTGGATAGTGATTTGCTAGAATTTTTGCTCCAGAGTCAGAGCTTGCTTACTATTTGTTTGGAAGACTACTGGTCTGCTTATGATAAATCAAGTGATTGGTCAATGATTGCAGACTGTGGTTGTGTTGGAACCAAGGTATCAACAATTGCCACGAAGGTATCTTTAACAATGGACGCACATGATGCACAATGGGAGCCAAGTCACTTAAAAACCGCTGGAGATAGTGAAAAGCATAGGTCTAAACACAGTCTTCAACTGAGAACGGCTCCACGCTTGCTTGGGATGGAAAGCATAACGTCTATGGAGGAGGGCAGTGCCAGAAAACAAAGACATCTCCATCGAATATTGATTAAAAAGTATAGCGATCACTTGGTATCTACAAGCTCTGATGTATGGATGGGTACTCACTTGCTTTTCATAGACCTCATGAGTGCTTTGGAGCTGCTCCTGAAACAATTGCGTGGCCACAAGGTTTCAAGGAGGGAAAGAAATAAGTTACAAAGGACACTAAATGACATTACTTCACTTGTTCCAGTTACAATTCTAATGCTACTTCCTGTATCTGCTGTAGGTCATGCTGCCATTTTTGCTGCAATCAACAAGTACATACCAGGCTTGATCCCTTCTCCGTATTCTTCTGAGCGGCTTGATGTTGTGAAACAACTAAAGAGAACCAAGAAGATGGAAGTTAGGACATGGAGCAACCTTGAAGATCCACCCTCTAGAATAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LETM1

Protein Analysis

720

Amino Acids

81.05

Weight (kDa)

7.87

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LETM1_RBD PF07766 635 - 707 8.8e-06 LETM1-like, RBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 2071
AccI GTMKAC 1 cut(s) 1386
AciI CCGC 3 cut(s) 1337, 1641, 2071
AclWI GGATC 3 cut(s) 840, 2041, 2135
AcoI YGGCCR 3 cut(s) 732, 876, 1891
AcsI RAATTY 5 cut(s) 290, 296, 959, 1033, 1448
AfaI GTAC 4 cut(s) 109, 701, 1831, 2033
AfiI CCNNNNNNNGG 1 cut(s) 322
AflII CTTAAG 1 cut(s) 1151
AhdI GACNNNNNGTC 2 cut(s) 907, 1498
AjnI CCWGG 5 cut(s) 391, 769, 1021, 1428, 2038
AjuI GAANNNNNNNTTGG 2 cut(s) 951, 983
AluBI AGCT 8 cut(s) 230, 348, 741, 826, 1167, 1470, 1812, 1870
AluI AGCT 8 cut(s) 230, 348, 741, 826, 1167, 1470, 1812, 1870
Alw21I GWGCWC 1 cut(s) 828
Alw26I GTCTC 3 cut(s) 21, 152, 1300
AlwI GGATC 3 cut(s) 840, 2041, 2135
AlwNI CAGNNNCTG 1 cut(s) 744
AoxI GGCC 3 cut(s) 732, 876, 1891
ApeKI GCWGC 9 cut(s) 263, 328, 741, 744, 749, 1277, 1870, 2006, 2018
ApoI RAATTY 5 cut(s) 290, 296, 959, 1033, 1448
AspLEI GCGC 2 cut(s) 370, 749
AspS9I GGNCC 1 cut(s) 767
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 251
AvaII GGWCC 1 cut(s) 767
BaeI ACNNNNGTAYC 4 cut(s) 1548, 1569, 1581, 1602
BalI TGGCCA 3 cut(s) 734, 878, 1893
BanII GRGCYC 1 cut(s) 828
BbsI GAAGAC 4 cut(s) 186, 1106, 1497, 1670
Bbv12I GWGCWC 1 cut(s) 828
BbvI GCAGC 9 cut(s) 250, 315, 728, 731, 736, 1264, 1857, 1993, 2005
BccI CCATC 6 cut(s) 743, 1254, 1705, 1773, 1819, 2104
BceAI ACGGC 2 cut(s) 458, 1707
BciT130I CCWGG 5 cut(s) 393, 771, 1023, 1430, 2040
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 3 cut(s) 21, 152, 1300
BfaI CTAG 9 cut(s) 168, 201, 342, 371, 510, 539, 1104, 1445, 2151
BfmI CTRYAG 4 cut(s) 216, 264, 750, 1995
BfoI RGCGCY 1 cut(s) 371
BfrI CTTAAG 1 cut(s) 1151
BglI GCCNNNNNGGC 1 cut(s) 884
BlpI GCTNAGC 1 cut(s) 935
Bme1390I CCNGG 6 cut(s) 305, 393, 771, 1023, 1430, 2040
Bme18I GGWCC 1 cut(s) 767
BmeRI GACNNNNNGTC 2 cut(s) 907, 1498
BmgT120I GGNCC 1 cut(s) 767
BmiI GGNNCC 4 cut(s) 768, 1558, 1623, 1695
BmrFI CCNGG 6 cut(s) 305, 393, 771, 1023, 1430, 2040
BmsI GCATC 2 cut(s) 97, 1600
BpiI GAAGAC 4 cut(s) 186, 1106, 1497, 1670
BplI GAGNNNNNCTC 2 cut(s) 1678, 1710
BpmI CTGGAG 2 cut(s) 1442, 1665
Bpu10I CCTNAGC 1 cut(s) 1163
Bpu1102I GCTNAGC 1 cut(s) 935
BpuMI CCSGG 1 cut(s) 305
BsaJI CCNNGG 5 cut(s) 392, 650, 770, 1428, 1560
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse1I ACTGG 2 cut(s) 1502, 1961
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 5 cut(s) 393, 771, 1023, 1430, 2040
BseDI CCNNGG 5 cut(s) 392, 650, 770, 1428, 1560
BseGI GGATG 5 cut(s) 47, 817, 1265, 1716, 1830
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 3 cut(s) 627, 1676, 2058
BseNI ACTGG 2 cut(s) 1502, 1961
BseRI GAGGAG 1 cut(s) 1748
BseXI GCAGC 9 cut(s) 250, 315, 728, 731, 736, 1264, 1857, 1993, 2005
BsgI GTGCAG 1 cut(s) 124
BshFI GGCC 3 cut(s) 734, 878, 1893
BsiHKAI GWGCWC 1 cut(s) 828
BsiSI CCGG 1 cut(s) 304
BslFI GGGAC 3 cut(s) 895, 1089, 1411
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 3 cut(s) 21, 152, 1300
BsmBI CGTCTC 1 cut(s) 1300
BsmFI GGGAC 3 cut(s) 895, 1089, 1411
BsnI GGCC 3 cut(s) 734, 878, 1893
Bsp1286I GDGCHC 1 cut(s) 828
Bsp143I GATC 6 cut(s) 564, 832, 1144, 1792, 2046, 2140
Bsp1720I GCTNAGC 1 cut(s) 935
BspACI CCGC 3 cut(s) 1337, 1641, 2071
BspANI GGCC 3 cut(s) 734, 878, 1893
BspCNI CTCAG 3 cut(s) 626, 1677, 2059
BspHI TCATGA 2 cut(s) 1039, 1854
BspLI GGNNCC 4 cut(s) 768, 1558, 1623, 1695
BspMAI CTGCAG 2 cut(s) 268, 754
BspPI GGATC 3 cut(s) 840, 2041, 2135
BspTI CTTAAG 1 cut(s) 1151
BsrBI CCGCTC 1 cut(s) 2071
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 2 cut(s) 1502, 1961
BssECI CCNNGG 5 cut(s) 392, 650, 770, 1428, 1560
BssMI GATC 6 cut(s) 564, 832, 1144, 1792, 2046, 2140
BssT1I CCWWGG 1 cut(s) 1560
Bst2UI CCWGG 5 cut(s) 393, 771, 1023, 1430, 2040
Bst6I CTCTTC 2 cut(s) 538, 945
BstAFI CTTAAG 1 cut(s) 1151
BstAPI GCANNNNNTGC 2 cut(s) 467, 1610
BstC8I GCNNGC 3 cut(s) 1028, 1472, 1703
BstDEI CTNAG 5 cut(s) 613, 935, 1163, 1685, 2067
BstDSI CCRYGG 1 cut(s) 650
BstF5I GGATG 5 cut(s) 47, 817, 1265, 1716, 1830
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 2 cut(s) 370, 749
BstKTI GATC 6 cut(s) 567, 835, 1147, 1795, 2049, 2143
BstMAI GTCTC 3 cut(s) 21, 152, 1300
BstMBI GATC 6 cut(s) 564, 832, 1144, 1792, 2046, 2140
BstNI CCWGG 5 cut(s) 393, 771, 1023, 1430, 2040
BstNSI RCATGY 2 cut(s) 431, 1284
BstSCI CCNGG 6 cut(s) 303, 391, 769, 1021, 1428, 2038
BstSFI CTRYAG 4 cut(s) 216, 264, 750, 1995
BstV1I GCAGC 9 cut(s) 250, 315, 728, 731, 736, 1264, 1857, 1993, 2005
BstV2I GAAGAC 4 cut(s) 186, 1106, 1497, 1670
BstX2I RGATCY 2 cut(s) 832, 2140
BstXI CCANNNNNNTGG 1 cut(s) 2110
BstYI RGATCY 2 cut(s) 832, 2140
BsuRI GGCC 3 cut(s) 734, 878, 1893
BtgI CCRYGG 1 cut(s) 650
BtsCI GGATG 5 cut(s) 47, 817, 1265, 1716, 1830
BtsI GCAGTG 4 cut(s) 273, 759, 1224, 1747
BtsIMutI CAGTG 5 cut(s) 273, 532, 759, 1224, 1747
Cac8I GCNNGC 3 cut(s) 1028, 1472, 1703
CaiI CAGNNNCTG 1 cut(s) 744
CciI TCATGA 2 cut(s) 1039, 1854
CfoI GCGC 2 cut(s) 370, 749
Cfr13I GGNCC 1 cut(s) 767
CseI GACGC 1 cut(s) 1610
Csp6I GTAC 4 cut(s) 108, 700, 1830, 2032
CviQI GTAC 4 cut(s) 108, 700, 1830, 2032
DdeI CTNAG 5 cut(s) 613, 935, 1163, 1685, 2067
DpnI GATC 6 cut(s) 566, 834, 1146, 1794, 2048, 2142
DpnII GATC 6 cut(s) 564, 832, 1144, 1792, 2046, 2140
DriI GACNNNNNGTC 2 cut(s) 907, 1498
EaeI YGGCCR 3 cut(s) 732, 876, 1891
Eam1104I CTCTTC 2 cut(s) 538, 945
Eam1105I GACNNNNNGTC 2 cut(s) 907, 1498
EarI CTCTTC 2 cut(s) 538, 945
EciI GGCGGA 1 cut(s) 1352
Ecl136II GAGCTC 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 1560
Eco24I GRGCYC 1 cut(s) 828
Eco32I GATATC 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 767
Eco53kI GAGCTC 1 cut(s) 826
EcoICRI GAGCTC 1 cut(s) 826
EcoO109I RGGNCCY 1 cut(s) 767
EcoRI GAATTC 1 cut(s) 290
EcoRII CCWGG 5 cut(s) 391, 769, 1021, 1428, 2038
EcoRV GATATC 1 cut(s) 550
EcoT14I CCWWGG 1 cut(s) 1560
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 828
ErhI CCWWGG 1 cut(s) 1560
Esp3I CGTCTC 1 cut(s) 1300
FaqI GGGAC 3 cut(s) 895, 1089, 1411
FblI GTMKAC 1 cut(s) 1386
FokI GGATG 5 cut(s) 34, 824, 1272, 1723, 1837
FriOI GRGCYC 1 cut(s) 828
FspBI CTAG 9 cut(s) 168, 201, 342, 371, 510, 539, 1104, 1445, 2151
GlaI GCGC 2 cut(s) 369, 748
GsuI CTGGAG 2 cut(s) 1442, 1665
HaeII RGCGCY 1 cut(s) 371
HaeIII GGCC 3 cut(s) 734, 878, 1893
HapII CCGG 1 cut(s) 304
HgaI GACGC 1 cut(s) 1610
HhaI GCGC 2 cut(s) 370, 749
Hin6I GCGC 2 cut(s) 368, 747
HinP1I GCGC 2 cut(s) 368, 747
HincII GTYRAC 1 cut(s) 1119
HindII GTYRAC 1 cut(s) 1119
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 6 cut(s) 335, 530, 592, 1018, 1330, 1462
HpaII CCGG 1 cut(s) 304
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 4 cut(s) 515, 581, 1119, 1387
Hpy188I TCNGA 4 cut(s) 616, 1467, 1816, 2068
Hpy188III TCNNGA 9 cut(s) 168, 201, 1040, 1301, 1392, 1459, 1855, 1876, 2151
Hpy8I GTNNAC 4 cut(s) 515, 581, 1119, 1387
Hpy99I CGWCG 1 cut(s) 522
HpyAV CCTTC 5 cut(s) 189, 412, 1089, 1576, 2061
HpyCH4IV ACGT 2 cut(s) 517, 1724
HpyF3I CTNAG 5 cut(s) 613, 935, 1163, 1685, 2067
HpySE526I ACGT 2 cut(s) 517, 1724
HspAI GCGC 2 cut(s) 368, 747
Kzo9I GATC 6 cut(s) 564, 832, 1144, 1792, 2046, 2140
LmnI GCTCC 7 cut(s) 345, 1461, 1621, 1699, 1867, 1878, 2127
Lsp1109I GCAGC 9 cut(s) 250, 315, 728, 731, 736, 1264, 1857, 1993, 2005
LweI GCATC 2 cut(s) 97, 1600
MaeI CTAG 9 cut(s) 168, 201, 342, 371, 510, 539, 1104, 1445, 2151
MaeII ACGT 2 cut(s) 517, 1724
MaeIII GTNAC 8 cut(s) 52, 173, 521, 527, 1243, 1628, 1923, 1963
MalI GATC 6 cut(s) 566, 834, 1146, 1794, 2048, 2142
MbiI CCGCTC 1 cut(s) 2071
MboI GATC 6 cut(s) 564, 832, 1144, 1792, 2046, 2140
MboII GAAGA 9 cut(s) 186, 525, 962, 1111, 1502, 1670, 2055, 2119, 2150
MfeI CAATTG 5 cut(s) 310, 360, 596, 1572, 1883
MflI RGATCY 2 cut(s) 832, 2140
MhlI GDGCHC 1 cut(s) 828
MlsI TGGCCA 3 cut(s) 734, 878, 1893
MluNI TGGCCA 3 cut(s) 734, 878, 1893
MlyI GAGTC 3 cut(s) 524, 601, 1471
MmeI TCCRAC 1 cut(s) 1534
Mox20I TGGCCA 3 cut(s) 734, 878, 1893
Mph1103I ATGCAT 1 cut(s) 433
MscI TGGCCA 3 cut(s) 734, 878, 1893
MseI TTAA 7 cut(s) 300, 554, 680, 1152, 1592, 1634, 1779
MslI CAYNNNNRTG 2 cut(s) 285, 1217
Msp20I TGGCCA 3 cut(s) 734, 878, 1893
MspA1I CMGCKG 2 cut(s) 741, 1643
MspCI CTTAAG 1 cut(s) 1151
MspI CCGG 1 cut(s) 304
MspR9I CCNGG 6 cut(s) 305, 393, 771, 1023, 1430, 2040
MunI CAATTG 5 cut(s) 310, 360, 596, 1572, 1883
MvaI CCWGG 5 cut(s) 393, 771, 1023, 1430, 2040
NciI CCSGG 1 cut(s) 305
NdeII GATC 6 cut(s) 564, 832, 1144, 1792, 2046, 2140
NlaIV GGNNCC 4 cut(s) 768, 1558, 1623, 1695
NmuCI GTSAC 2 cut(s) 527, 1628
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 2 cut(s) 431, 1284
PagI TCATGA 2 cut(s) 1039, 1854
PfeI GAWTC 3 cut(s) 335, 1018, 1330
PleI GAGTC 3 cut(s) 524, 600, 1470
PpsI GAGTC 3 cut(s) 524, 600, 1470
PpuMI RGGWCCY 1 cut(s) 767
Psp124BI GAGCTC 1 cut(s) 828
Psp5II RGGWCCY 1 cut(s) 767
Psp6I CCWGG 5 cut(s) 391, 769, 1021, 1428, 2038
PspGI CCWGG 5 cut(s) 391, 769, 1021, 1428, 2038
PspN4I GGNNCC 4 cut(s) 768, 1558, 1623, 1695
PspPI GGNCC 1 cut(s) 767
PspPPI RGGWCCY 1 cut(s) 767
PsrI GAACNNNNNNTAC 2 cut(s) 1214, 1246
PstI CTGCAG 2 cut(s) 268, 754
PstNI CAGNNNCTG 1 cut(s) 744
PsuI RGATCY 2 cut(s) 832, 2140
PvuII CAGCTG 1 cut(s) 741
RsaI GTAC 4 cut(s) 109, 701, 1831, 2033
RsaNI GTAC 4 cut(s) 108, 700, 1830, 2032
RseI CAYNNNNRTG 2 cut(s) 285, 1217
SacI GAGCTC 1 cut(s) 828
SaqAI TTAA 7 cut(s) 300, 554, 680, 1152, 1592, 1634, 1779
Sau3AI GATC 6 cut(s) 564, 832, 1144, 1792, 2046, 2140
Sau96I GGNCC 1 cut(s) 767
SchI GAGTC 3 cut(s) 524, 601, 1471
ScrFI CCNGG 6 cut(s) 305, 393, 771, 1023, 1430, 2040
SduI GDGCHC 1 cut(s) 828
SfaNI GCATC 2 cut(s) 97, 1600
SfcI CTRYAG 4 cut(s) 216, 264, 750, 1995
SinI GGWCC 1 cut(s) 767
SmiMI CAYNNNNRTG 2 cut(s) 285, 1217
SmlI CTYRAG 1 cut(s) 1151
SmoI CTYRAG 1 cut(s) 1151
SsiI CCGC 3 cut(s) 1337, 1641, 2071
SspI AATATT 1 cut(s) 1773
SspMI CTAG 9 cut(s) 168, 201, 342, 371, 510, 539, 1104, 1445, 2151
SstI GAGCTC 1 cut(s) 828
StyD4I CCNGG 6 cut(s) 303, 391, 769, 1021, 1428, 2038
StyI CCWWGG 1 cut(s) 1560
TaiI ACGT 2 cut(s) 520, 1727
TaqI TCGA 1 cut(s) 1768
TatI WGTACW 2 cut(s) 107, 2031
TauI GCSGC 1 cut(s) 2074
TfiI GAWTC 3 cut(s) 335, 1018, 1330
Tru1I TTAA 7 cut(s) 300, 554, 680, 1152, 1592, 1634, 1779
Tru9I TTAA 7 cut(s) 300, 554, 680, 1152, 1592, 1634, 1779
TscAI CASTG 5 cut(s) 273, 532, 759, 1224, 1747
TseFI GTSAC 2 cut(s) 527, 1628
TseI GCWGC 9 cut(s) 263, 328, 741, 744, 749, 1277, 1870, 2006, 2018
Tsp45I GTSAC 2 cut(s) 527, 1628
TspGWI ACGGA 3 cut(s) 40, 667, 2046
TspRI CASTG 5 cut(s) 273, 532, 759, 1224, 1747
Vha464I CTTAAG 1 cut(s) 1151
VpaK11BI GGWCC 1 cut(s) 767
XapI RAATTY 5 cut(s) 290, 296, 959, 1033, 1448
XbaI TCTAGA 3 cut(s) 167, 200, 2150
XceI RCATGY 2 cut(s) 431, 1284
XmiI GTMKAC 1 cut(s) 1386
XspI CTAG 9 cut(s) 168, 201, 342, 371, 510, 539, 1104, 1445, 2151
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.