pycom10g04740

ribosome-binding factor A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012528
Physical Location & Seq
Forward (+)
4976823 .. 4977692
870 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
ATGCCAAACTTGGTCCTCCACCACCCACCACCACTCACCCCCTTTCTTTCCCACCTCCCATCATCATCATCATCAAGCTTTCGAATTCGGACCCAGAAATTATCGGCCTCGCTTCCCCCAAAAAATCCTGCGGTTGTACTCCGTTGGCGGCCCAACACCATCAGGTGCATGGCCAACCCGCACAGAGTGGCAATGGTGGCCAAGCAGATAAGGAGGGAGCTGTCTGACATGCTTATAACTGATAAGGTCTTGCAGTACGCTATTCTTCCTGAGGTTTCTTTGGGTGCTGACCGTTTCCTCTCGTCGCTAACCACCATAAGCGATGTGGAAGTCTCCACCGACTTGCAGGTGGTTAAAGTGTATGTTTCTATTTTTGGCGATGAGAGAGGGAAGGAGGTTGCCCTTGCTGGGCTAAAGTCAAAAGCCAAATATGTCCGAGGTCAATTGGGAAGGCGTATGAAGCTGCGGCTGACTCCTGAGATACGCTTCATAGAAGATGAGTCATTAGAGAGAGGAAGCAGGGTAAACTTAATACCGTACTATTTCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009507 GO:0009536 GO:0022613 GO:0042254 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RBFA

Protein Analysis

184

Amino Acids

20.69

Weight (kDa)

10.16

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBFA PF02033 62 - 176 5.7e-26 Ribosome-binding factor A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34730 AT4G34730 AT4G34730
fragaria_vesca FvH4_2g10740 FvH4_2g10740
malus_domestica MD00G1066800.v1.1
prunus_persica Prupe.8G082300_v2.0.a1
pyrus_communis pycom05g04460 pycom10g04740
rosa_chinensis RchiOBHm_Chr6g0268531
rosa_laevigata RLG00000013905
rosa_multiflora Rmu_sc0012389.1_g000002
rosa_roxburghii Rroxscaffold_7G00199750
rosa_rugosa Rorug06G0042900
rosa_samantha Rh6AG162300 Rh6BG166500 Rh6CG160300 Rh6DG151700
rosa_wichuraiana Rw6G014010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 236
AarI CACCTGC 1 cut(s) 337
Acc36I ACCTGC 1 cut(s) 337
AciI CCGC 4 cut(s) 131, 148, 179, 466
AcoI YGGCCR 2 cut(s) 171, 198
AcsI RAATTY 1 cut(s) 84
AdeI CACNNNGTG 1 cut(s) 187
AfaI GTAC 3 cut(s) 138, 257, 539
AfiI CCNNNNNNNGG 1 cut(s) 408
AluBI AGCT 3 cut(s) 78, 220, 463
AluI AGCT 3 cut(s) 78, 220, 463
Alw26I GTCTC 1 cut(s) 337
AoxI GGCC 4 cut(s) 105, 149, 171, 198
ApeKI GCWGC 1 cut(s) 463
ApoI RAATTY 1 cut(s) 84
AspS9I GGNCC 3 cut(s) 13, 90, 150
AsuHPI GGTGA 1 cut(s) 28
AsuII TTCGAA 1 cut(s) 82
AvaII GGWCC 2 cut(s) 13, 90
AxyI CCTNAGG 1 cut(s) 270
BalI TGGCCA 2 cut(s) 173, 200
BbvI GCAGC 1 cut(s) 450
BccI CCATC 2 cut(s) 67, 167
BcoDI GTCTC 1 cut(s) 337
BfuAI ACCTGC 1 cut(s) 337
BisI GCNGC 3 cut(s) 149, 464, 467
BlsI GCNGC 3 cut(s) 150, 465, 468
Bme18I GGWCC 2 cut(s) 13, 90
BmgT120I GGNCC 3 cut(s) 13, 90, 150
BmiI GGNNCC 1 cut(s) 92
Bpu14I TTCGAA 1 cut(s) 82
BsaJI CCNNGG 1 cut(s) 436
Bsc4I CCNNNNNNNGG 1 cut(s) 408
Bse21I CCTNAGG 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 436
BseLI CCNNNNNNNGG 1 cut(s) 408
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 2 cut(s) 261, 468
BseXI GCAGC 1 cut(s) 450
BseYI CCCAGC 1 cut(s) 407
BshFI GGCC 4 cut(s) 107, 151, 173, 200
BslI CCNNNNNNNGG 1 cut(s) 408
BsmAI GTCTC 1 cut(s) 337
BsnI GGCC 4 cut(s) 107, 151, 173, 200
Bsp119I TTCGAA 1 cut(s) 82
BspACI CCGC 4 cut(s) 131, 148, 179, 466
BspANI GGCC 4 cut(s) 107, 151, 173, 200
BspCNI CTCAG 2 cut(s) 262, 469
BspLI GGNNCC 1 cut(s) 92
BspMI ACCTGC 1 cut(s) 337
BspT104I TTCGAA 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 436
Bst4CI ACNGT 2 cut(s) 293, 537
BstBI TTCGAA 1 cut(s) 82
BstDEI CTNAG 2 cut(s) 270, 477
BstMAI GTCTC 1 cut(s) 337
BstMWI GCNNNNNNNGC 2 cut(s) 197, 460
BstNSI RCATGY 1 cut(s) 232
BstV1I GCAGC 1 cut(s) 450
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 4 cut(s) 107, 151, 173, 200
BtgZI GCGATG 2 cut(s) 336, 393
BveI ACCTGC 1 cut(s) 337
Cfr13I GGNCC 3 cut(s) 13, 90, 150
Csp6I GTAC 3 cut(s) 137, 256, 538
CviAII CATG 2 cut(s) 169, 229
CviQI GTAC 3 cut(s) 137, 256, 538
DdeI CTNAG 2 cut(s) 270, 477
DraIII CACNNNGTG 1 cut(s) 187
EaeI YGGCCR 2 cut(s) 171, 198
Eco47I GGWCC 2 cut(s) 13, 90
Eco81I CCTNAGG 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 84
FaeI CATG 2 cut(s) 172, 232
FatI CATG 2 cut(s) 168, 228
FauI CCCGC 1 cut(s) 186
FauNDI CATATG 1 cut(s) 548
Fnu4HI GCNGC 3 cut(s) 149, 464, 467
Fsp4HI GCNGC 3 cut(s) 149, 464, 467
GluI GCNGC 3 cut(s) 149, 464, 467
GsaI CCCAGC 1 cut(s) 411
HaeIII GGCC 4 cut(s) 107, 151, 173, 200
Hin1II CATG 2 cut(s) 172, 232
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 2 cut(s) 472, 500
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 3 cut(s) 90, 226, 437
Hpy188III TCNNGA 2 cut(s) 269, 476
Hpy8I GTNNAC 1 cut(s) 526
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 2 cut(s) 385, 444
HpyCH4III ACNGT 2 cut(s) 293, 537
HpyCH4V TGCA 3 cut(s) 168, 253, 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 460
HpyF3I CTNAG 2 cut(s) 270, 477
Hsp92II CATG 2 cut(s) 172, 232
LmnI GCTCC 1 cut(s) 217
LpnPI CCDG 8 cut(s) 107, 141, 148, 282, 332, 393, 489, 505
Lsp1109I GCAGC 1 cut(s) 450
MboII GAAGA 2 cut(s) 257, 506
MfeI CAATTG 1 cut(s) 443
MlsI TGGCCA 2 cut(s) 173, 200
MluCI AATT 3 cut(s) 84, 98, 443
MluNI TGGCCA 2 cut(s) 173, 200
MlyI GAGTC 2 cut(s) 466, 509
Mox20I TGGCCA 2 cut(s) 173, 200
MscI TGGCCA 2 cut(s) 173, 200
MseI TTAA 2 cut(s) 354, 530
Msp20I TGGCCA 2 cut(s) 173, 200
MunI CAATTG 1 cut(s) 443
MwoI GCNNNNNNNGC 2 cut(s) 197, 460
NdeI CATATG 1 cut(s) 548
NlaIII CATG 2 cut(s) 172, 232
NlaIV GGNNCC 1 cut(s) 92
NspI RCATGY 1 cut(s) 232
NspV TTCGAA 1 cut(s) 82
PaqCI CACCTGC 1 cut(s) 337
PkrI GCNGC 3 cut(s) 150, 465, 468
PleI GAGTC 2 cut(s) 466, 508
PpsI GAGTC 2 cut(s) 466, 508
PsiI TTATAA 1 cut(s) 236
PspFI CCCAGC 1 cut(s) 407
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 3 cut(s) 13, 90, 150
RsaI GTAC 3 cut(s) 138, 257, 539
RsaNI GTAC 3 cut(s) 137, 256, 538
SaqAI TTAA 2 cut(s) 354, 530
SatI GCNGC 3 cut(s) 149, 464, 467
Sau96I GGNCC 3 cut(s) 13, 90, 150
SchI GAGTC 2 cut(s) 466, 509
SfuI TTCGAA 1 cut(s) 82
SinI GGWCC 2 cut(s) 13, 90
Sse9I AATT 3 cut(s) 84, 98, 443
SsiI CCGC 4 cut(s) 131, 148, 179, 466
TaaI ACNGT 2 cut(s) 293, 537
TaqI TCGA 1 cut(s) 82
TasI AATT 3 cut(s) 84, 98, 443
TatI WGTACW 1 cut(s) 136
TauI GCSGC 2 cut(s) 151, 469
Tru1I TTAA 2 cut(s) 354, 530
Tru9I TTAA 2 cut(s) 354, 530
TseI GCWGC 1 cut(s) 463
TspDTI ATGAA 3 cut(s) 473, 478, 535
TspGWI ACGGA 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 13, 90
XapI RAATTY 1 cut(s) 84
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 2 cut(s) 166, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.