pycom10g05740

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014184
Physical Location & Seq
Forward (+)
6263451 .. 6264134
684 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGGATTCAGTCAATTTTTATGACATCAAAACTGAGAAGGCGAACGCAATCTGGAAATACCGCCAGCTTCGAAAACTCGCAAACTTGTTTCGGTTGATTGAGGTGCTTGCTTTTCTGCTCCTGCTTTCCAGGTTTTCTGTCCAACTCCCTCATGCTGTCAAGAACTCTGGCTCTTACTTCAAAGACATATCCGGCTTCATGGTAAGTCCTCGCTTCGTGTTTGTCATTGGAAATGTAATAGTTATCGTTCTCTTCGCGAAGTCGGGCAGATTTTCAGCTAAAGACTCGAGCACAGGCAGCTTGGGCGATGATCTTTATGAAGAGTTTGTTCAGAACAGCGAAAAAAATCAGAAACTTCGTGGCGCTGTGATTGAGTACCAGGCCATAAAAAACACACCTAAGGATAGTATAGTTTCTGAGCAAGCTCCTCAAACTTTGCAATTCAAGAACTTCACAAGGAGTCAGTCAGAGAATTTAGAACGCGAGGTTTGCAAGAGCCCGCGGCGCTTATTGAAACGATCGGAGACAGAGAAGTTCAAGAAAAACGTTCAGCCTGGTGGGAAGCCGGCAGGGAGTGTGTATCCTGAAGACGGCATGAGTAACGAGGAGTTTCGGAGCAAGGTTGAGTCGTTCATTGCAAGGCAGCAGAAGTTTCGACTGCAAGAAGGGTTCTCTGTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

228

Amino Acids

25.96

Weight (kDa)

9.71

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34560
fragaria_vesca FvH4_2g09200
malus_domestica MD05G1064000.v1.1 MD10G1072700.v1.1
prunus_persica Prupe.6G200500_v2.0.a1 Prupe.8G094100_v2.0.a1
pyrus_communis pycom10g05740
rosa_chinensis RchiOBHm_Chr6g0265051
rosa_laevigata RLG00000014213
rosa_roxburghii Rroxscaffold_175G00432120 Rroxscaffold_7G00203000
rosa_rugosa Rorug06G0013400
rosa_samantha Rh6AG136400 Rh6BG133400 Rh6CG131800 Rh6DG119100
rosa_wichuraiana Rw6G011770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 257, 483, 502
AciI CCGC 3 cut(s) 61, 500, 502
AclI AACGTT 1 cut(s) 546
AcsI RAATTY 1 cut(s) 472
AcuI CTGAAG 1 cut(s) 606
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 1 cut(s) 590
AgsI TTSAA 4 cut(s) 181, 445, 514, 538
AjnI CCWGG 3 cut(s) 128, 378, 553
AluBI AGCT 4 cut(s) 67, 278, 300, 425
AluI AGCT 4 cut(s) 67, 278, 300, 425
Alw21I GWGCWC 1 cut(s) 293
Alw26I GTCTC 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 286
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 2 cut(s) 297, 643
ApoI RAATTY 1 cut(s) 472
AspLEI GCGC 2 cut(s) 365, 507
AsuII TTCGAA 1 cut(s) 70
AvaI CYCGRG 1 cut(s) 286
AxyI CCTNAGG 1 cut(s) 399
BanII GRGCYC 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 293
BbvI GCAGC 2 cut(s) 309, 655
BceAI ACGGC 1 cut(s) 607
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BciT130I CCWGG 3 cut(s) 130, 380, 555
BciVI GTATCC 1 cut(s) 591
BcoDI GTCTC 1 cut(s) 518
BfaI CTAG 1 cut(s) 682
BfoI RGCGCY 2 cut(s) 366, 508
BfuI GTATCC 1 cut(s) 591
BisI GCNGC 3 cut(s) 298, 503, 644
BlsI GCNGC 3 cut(s) 299, 504, 645
Bme1390I CCNGG 3 cut(s) 130, 380, 555
BmeT110I CYCGRG 1 cut(s) 286
BmrFI CCNGG 3 cut(s) 130, 380, 555
BpiI GAAGAC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 70
BsaJI CCNNGG 1 cut(s) 500
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse118I RCCGGY 1 cut(s) 565
Bse21I CCTNAGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 3 cut(s) 130, 380, 555
BseDI CCNNGG 1 cut(s) 500
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 2 cut(s) 24, 408
BseRI GAGGAG 2 cut(s) 417, 620
BseXI GCAGC 2 cut(s) 309, 655
Bsh1236I CGCG 3 cut(s) 257, 483, 502
Bsh1285I CGRYCG 1 cut(s) 521
BshFI GGCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 521
BsiHKAI GWGCWC 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 286
BsiSI CCGG 2 cut(s) 192, 566
BslI CCNNNNNNNGG 1 cut(s) 590
BsmAI GTCTC 1 cut(s) 518
BsnI GGCC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 286
Bsp119I TTCGAA 1 cut(s) 70
Bsp1286I GDGCHC 2 cut(s) 293, 500
Bsp143I GATC 2 cut(s) 310, 518
Bsp68I TCGCGA 1 cut(s) 257
BspACI CCGC 3 cut(s) 61, 500, 502
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 2 cut(s) 25, 409
BspFNI CGCG 3 cut(s) 257, 483, 502
BspT104I TTCGAA 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 633
BsrFI RCCGGY 1 cut(s) 565
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 500
BssMI GATC 2 cut(s) 310, 518
Bst2UI CCWGG 3 cut(s) 130, 380, 555
Bst6I CTCTTC 2 cut(s) 257, 315
BstBI TTCGAA 1 cut(s) 70
BstC8I GCNNGC 5 cut(s) 65, 108, 423, 500, 567
BstDEI CTNAG 3 cut(s) 33, 399, 417
BstDSI CCRYGG 1 cut(s) 500
BstFNI CGCG 3 cut(s) 257, 483, 502
BstH2I RGCGCY 2 cut(s) 366, 508
BstHHI GCGC 2 cut(s) 365, 507
BstKTI GATC 2 cut(s) 313, 521
BstMAI GTCTC 1 cut(s) 518
BstMBI GATC 2 cut(s) 310, 518
BstMCI CGRYCG 1 cut(s) 521
BstMWI GCNNNNNNNGC 4 cut(s) 297, 303, 489, 504
BstNI CCWGG 3 cut(s) 130, 380, 555
BstSCI CCNGG 3 cut(s) 128, 378, 553
BstUI CGCG 3 cut(s) 257, 483, 502
BstV1I GCAGC 2 cut(s) 309, 655
BstV2I GAAGAC 1 cut(s) 594
Bsu36I CCTNAGG 1 cut(s) 399
BsuI GTATCC 1 cut(s) 591
BsuRI GGCC 1 cut(s) 383
BtgI CCRYGG 1 cut(s) 500
BtgZI GCGATG 1 cut(s) 321
BtuMI TCGCGA 1 cut(s) 257
Cac8I GCNNGC 5 cut(s) 65, 108, 423, 500, 567
CfoI GCGC 2 cut(s) 365, 507
Cfr10I RCCGGY 1 cut(s) 565
Cfr42I CCGCGG 1 cut(s) 503
Csp6I GTAC 1 cut(s) 376
CviAII CATG 3 cut(s) 152, 199, 595
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 3 cut(s) 33, 399, 417
DpnI GATC 2 cut(s) 312, 520
DpnII GATC 2 cut(s) 310, 518
Eam1104I CTCTTC 2 cut(s) 257, 315
EarI CTCTTC 2 cut(s) 257, 315
Eco24I GRGCYC 1 cut(s) 500
Eco57I CTGAAG 1 cut(s) 606
Eco81I CCTNAGG 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 286
EcoRII CCWGG 3 cut(s) 128, 378, 553
EcoT38I GRGCYC 1 cut(s) 500
FaeI CATG 3 cut(s) 155, 202, 598
FaiI YATR 8 cut(s) 21, 153, 188, 200, 318, 386, 410, 596
FatI CATG 3 cut(s) 151, 198, 594
FauI CCCGC 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 298, 503, 644
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 3 cut(s) 298, 503, 644
FspBI CTAG 1 cut(s) 682
GlaI GCGC 2 cut(s) 364, 506
GluI GCNGC 3 cut(s) 298, 503, 644
HaeII RGCGCY 2 cut(s) 366, 508
HaeIII GGCC 1 cut(s) 383
HapII CCGG 2 cut(s) 192, 566
HhaI GCGC 2 cut(s) 365, 507
Hin1II CATG 3 cut(s) 155, 202, 598
Hin6I GCGC 2 cut(s) 363, 505
HinP1I GCGC 2 cut(s) 363, 505
HinfI GANTC 4 cut(s) 5, 284, 460, 626
HpaII CCGG 2 cut(s) 192, 566
Hpy166II GTNNAC 1 cut(s) 679
Hpy188I TCNGA 6 cut(s) 333, 351, 418, 469, 523, 615
Hpy188III TCNNGA 6 cut(s) 52, 160, 256, 445, 538, 584
Hpy8I GTNNAC 1 cut(s) 679
HpyAV CCTTC 2 cut(s) 31, 659
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 4 cut(s) 439, 492, 638, 661
HpyF10VI GCNNNNNNNGC 4 cut(s) 297, 303, 489, 504
HpyF3I CTNAG 3 cut(s) 33, 399, 417
HpySE526I ACGT 1 cut(s) 546
Hsp92II CATG 3 cut(s) 155, 202, 598
HspAI GCGC 2 cut(s) 363, 505
KroI GCCGGC 1 cut(s) 565
KroNI GCCGGC 1 cut(s) 567
KspI CCGCGG 1 cut(s) 503
Kzo9I GATC 2 cut(s) 310, 518
LmnI GCTCC 3 cut(s) 123, 430, 615
Lsp1109I GCAGC 2 cut(s) 309, 655
MaeI CTAG 1 cut(s) 682
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 1 cut(s) 599
MalI GATC 2 cut(s) 312, 520
MboI GATC 2 cut(s) 310, 518
MboII GAAGA 3 cut(s) 244, 332, 599
MhlI GDGCHC 2 cut(s) 293, 500
MluCI AATT 3 cut(s) 13, 440, 472
MlyI GAGTC 3 cut(s) 278, 469, 635
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 6 cut(s) 94, 159, 219, 438, 478, 598
MroNI GCCGGC 1 cut(s) 565
MspA1I CMGCKG 1 cut(s) 502
MspI CCGG 2 cut(s) 192, 566
MspR9I CCNGG 3 cut(s) 130, 380, 555
MvaI CCWGG 3 cut(s) 130, 380, 555
MvnI CGCG 3 cut(s) 257, 483, 502
MwoI GCNNNNNNNGC 4 cut(s) 297, 303, 489, 504
NaeI GCCGGC 1 cut(s) 567
NdeII GATC 2 cut(s) 310, 518
NgoMIV GCCGGC 1 cut(s) 565
NlaIII CATG 3 cut(s) 155, 202, 598
NruI TCGCGA 1 cut(s) 257
NspV TTCGAA 1 cut(s) 70
PaeR7I CTCGAG 1 cut(s) 286
PdiI GCCGGC 1 cut(s) 567
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 3 cut(s) 299, 504, 645
Ple19I CGATCG 1 cut(s) 521
PleI GAGTC 3 cut(s) 278, 468, 634
PpsI GAGTC 3 cut(s) 278, 468, 634
Psp1406I AACGTT 1 cut(s) 546
Psp6I CCWGG 3 cut(s) 128, 378, 553
PspGI CCWGG 3 cut(s) 128, 378, 553
PspXI VCTCGAGB 1 cut(s) 286
PvuI CGATCG 1 cut(s) 521
RruI TCGCGA 1 cut(s) 257
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
SacII CCGCGG 1 cut(s) 503
SatI GCNGC 3 cut(s) 298, 503, 644
Sau3AI GATC 2 cut(s) 310, 518
SchI GAGTC 3 cut(s) 278, 469, 635
ScrFI CCNGG 3 cut(s) 130, 380, 555
SduI GDGCHC 2 cut(s) 293, 500
Sfr274I CTCGAG 1 cut(s) 286
Sfr303I CCGCGG 1 cut(s) 503
SfuI TTCGAA 1 cut(s) 70
SgrBI CCGCGG 1 cut(s) 503
SlaI CTCGAG 1 cut(s) 286
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
Sse9I AATT 3 cut(s) 13, 440, 472
SsiI CCGC 3 cut(s) 61, 500, 502
SspMI CTAG 1 cut(s) 682
StyD4I CCNGG 3 cut(s) 128, 378, 553
TaiI ACGT 1 cut(s) 549
TaqI TCGA 3 cut(s) 70, 287, 655
TasI AATT 3 cut(s) 13, 440, 472
TauI GCSGC 1 cut(s) 505
TfiI GAWTC 1 cut(s) 5
TseI GCWGC 2 cut(s) 297, 643
TspDTI ATGAA 3 cut(s) 187, 333, 622
XapI RAATTY 1 cut(s) 472
XhoI CTCGAG 1 cut(s) 286
XspI CTAG 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.