pycom10g05990

BTB POZ domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009651
Physical Location & Seq
Reverse (-)
6738771 .. 6739769
999 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g05990.1

Sequence Viewer

Length: 999 bp
ATGGTCACAGGTACCGGCTCCAAAAAGCGGCATCGCGCCGTACACCCCGCCAGCTCAGCCGCCGGCAGCAGTTCCAGCCGCCCTTGCGCCGCCGTCATCATCGGCTCCGCTGCCAAAAGACCCACCCATCCCGACCCAATCCCGAGCCCGACTCTGAAACCCGACCCCACCAACCCCTACTCCTTCGACGACCCTTCCTCCGCCGACGTCATTCTCCGCCTCTTCGTCGACCCCTCCGCGACGTCATTGTCTCTATCCCCCGCCACCACCCAGCCTCAGGATGACGTCGTTCTCCACCTCCACACCCACGCCCTCTTCCGCTGCAAGTACTTCGCCGCCCTCTTGTCCGACCGATGGCAGCACCCCGACTCCCAAGACGACGCCGTTCGCAAGATCAACCTCCGTGTCCCACCGACTCCCGGCGCCATGGAGGCCCGCATTTCCCTCCTCCAACTCCTCTACACCCCAGACCTTGCCTCCGCCATAACCACTGTGTCGACGGCGCTCGACCTTCTCCCCGTGGCTTCGGAGCTCATCTTCGATGATTGCGTTCGATTCTGCCTTCGATTCCTGGAGGCCGTCCCTTGGACCCAAGACGATGAAGCTCGAGTGCTCGCTCTAATCCCTCATCTGAGCGAAGACGAATCCAAAGACCTCATCGCTAGGGTTTCTGGAAGCCCCGATTCGAGCGAGGAGATGCTGTACGGTCTCATCATCGCCGTGACCCACAACCCGAACATGGCGTACGTCAAGGCCTTCGTCGCGAAGCTGCTCCGGGATTTCGGGTCCAGAGACCTGGTCGAGCGGGTTCTGGATCGGGCGTTTCAGACCACCTTCAAGGTCGTCAAGGAGTCCATGGAGGAGTACATGAGTCCCGGAGTCCGTGGCGACCATGACGAGACCGAGGCCATCCAGAGGCTCAACCTCCACACCGCATTGACCCATGGGAAGCACCTGGTGTGGCTCGTGGAGAGGATCGATCGAGCTCCGAGTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

333

Amino Acids

36.49

Weight (kDa)

5.89

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 247
AatII GACGTC 3 cut(s) 210, 245, 288
Acc65I GGTACC 1 cut(s) 11
AccB1I GGYRCC 2 cut(s) 11, 422
AccBSI CCGCTC 1 cut(s) 805
AccI GTMKAC 2 cut(s) 228, 497
AccII CGCG 3 cut(s) 36, 239, 764
AclWI GGATC 2 cut(s) 822, 983
AcyI GRCGYC 5 cut(s) 207, 242, 285, 381, 423
AdeI CACNNNGTG 1 cut(s) 958
AfaI GTAC 6 cut(s) 13, 42, 329, 704, 746, 866
AfiI CCNNNNNNNGG 7 cut(s) 27, 277, 354, 419, 585, 739, 915
AgsI TTSAA 1 cut(s) 838
AjnI CCWGG 3 cut(s) 570, 795, 954
AluBI AGCT 5 cut(s) 54, 532, 605, 769, 986
AluI AGCT 5 cut(s) 54, 532, 605, 769, 986
Alw21I GWGCWC 3 cut(s) 534, 615, 988
Alw26I GTCTC 4 cut(s) 255, 713, 786, 893
AlwI GGATC 2 cut(s) 822, 983
Ama87I CYCGRG 2 cut(s) 142, 606
AoxI GGCC 4 cut(s) 432, 576, 753, 906
ApeKI GCWGC 5 cut(s) 66, 110, 321, 358, 769
Asp718I GGTACC 1 cut(s) 11
AspLEI GCGC 4 cut(s) 38, 89, 425, 505
AspS9I GGNCC 3 cut(s) 433, 588, 786
AsuC2I CCSGG 3 cut(s) 420, 776, 876
AvaI CYCGRG 2 cut(s) 142, 606
AvaII GGWCC 2 cut(s) 588, 786
AxyI CCTNAGG 1 cut(s) 276
BanI GGYRCC 2 cut(s) 11, 422
BanII GRGCYC 3 cut(s) 149, 534, 988
BauI CACGAG 1 cut(s) 965
BbsI GAAGAC 1 cut(s) 645
Bbv12I GWGCWC 3 cut(s) 534, 615, 988
BbvI GCAGC 5 cut(s) 78, 97, 308, 370, 756
BccI CCATC 3 cut(s) 135, 348, 917
BceAI ACGGC 6 cut(s) 23, 77, 368, 516, 563, 704
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 3 cut(s) 572, 797, 956
BcnI CCSGG 3 cut(s) 420, 776, 876
BcoDI GTCTC 4 cut(s) 255, 713, 786, 893
BfaI CTAG 1 cut(s) 663
BfoI RGCGCY 2 cut(s) 426, 506
BglI GCCNNNNNGGC 1 cut(s) 431
BlpI GCTNAGC 1 cut(s) 55
BmcAI AGTACT 1 cut(s) 329
Bme1390I CCNGG 6 cut(s) 420, 572, 776, 797, 876, 956
Bme18I GGWCC 2 cut(s) 588, 786
BmeT110I CYCGRG 2 cut(s) 142, 606
BmgT120I GGNCC 3 cut(s) 433, 588, 786
BmiI GGNNCC 6 cut(s) 13, 19, 106, 424, 590, 787
BmrFI CCNGG 6 cut(s) 420, 572, 776, 797, 876, 956
BmsI GCATC 2 cut(s) 40, 687
BpiI GAAGAC 1 cut(s) 645
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BpmI CTGGAG 1 cut(s) 593
Bpu1102I GCTNAGC 1 cut(s) 55
BpuMI CCSGG 3 cut(s) 420, 776, 876
Bsa29I ATCGAT 1 cut(s) 978
BsaHI GRCGYC 5 cut(s) 207, 242, 285, 381, 423
BsaI GGTCTC 3 cut(s) 713, 786, 893
BsaJI CCNNGG 7 cut(s) 426, 519, 584, 855, 883, 903, 943
Bsc4I CCNNNNNNNGG 7 cut(s) 27, 277, 354, 419, 585, 739, 915
Bse118I RCCGGY 2 cut(s) 14, 62
Bse21I CCTNAGG 1 cut(s) 276
BseBI CCWGG 3 cut(s) 572, 797, 956
BseCI ATCGAT 1 cut(s) 978
BseDI CCNNGG 7 cut(s) 426, 519, 584, 855, 883, 903, 943
BseGI GGATG 3 cut(s) 127, 286, 909
BseLI CCNNNNNNNGG 7 cut(s) 27, 277, 354, 419, 585, 739, 915
BseMII CTCAG 3 cut(s) 69, 290, 623
BseRI GAGGAG 4 cut(s) 437, 446, 707, 875
BseXI GCAGC 5 cut(s) 78, 97, 308, 370, 756
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 3 cut(s) 36, 239, 764
Bsh1285I CGRYCG 2 cut(s) 352, 982
BshFI GGCC 4 cut(s) 434, 578, 755, 908
BshNI GGYRCC 2 cut(s) 11, 422
BshVI ATCGAT 1 cut(s) 978
BsiEI CGRYCG 2 cut(s) 352, 982
BsiHKAI GWGCWC 3 cut(s) 534, 615, 988
BsiHKCI CYCGRG 2 cut(s) 142, 606
BsiSI CCGG 5 cut(s) 15, 63, 420, 775, 876
BsiWI CGTACG 1 cut(s) 744
BslFI GGGAC 3 cut(s) 392, 566, 858
BslI CCNNNNNNNGG 7 cut(s) 27, 277, 354, 419, 585, 739, 915
BsmAI GTCTC 4 cut(s) 255, 713, 786, 893
BsmFI GGGAC 3 cut(s) 392, 566, 858
BsnI GGCC 4 cut(s) 434, 578, 755, 908
Bso31I GGTCTC 3 cut(s) 713, 786, 893
BsoBI CYCGRG 2 cut(s) 142, 606
Bsp1286I GDGCHC 4 cut(s) 149, 534, 615, 988
Bsp143I GATC 4 cut(s) 393, 814, 975, 979
Bsp1720I GCTNAGC 1 cut(s) 55
Bsp19I CCATGG 3 cut(s) 426, 855, 943
Bsp68I TCGCGA 1 cut(s) 764
BspANI GGCC 4 cut(s) 434, 578, 755, 908
BspCNI CTCAG 3 cut(s) 68, 289, 624
BspDI ATCGAT 1 cut(s) 978
BspFNI CGCG 3 cut(s) 36, 239, 764
BspLI GGNNCC 6 cut(s) 13, 19, 106, 424, 590, 787
BspPI GGATC 2 cut(s) 822, 983
BspT107I GGYRCC 2 cut(s) 11, 422
BspTNI GGTCTC 3 cut(s) 713, 786, 893
BsrBI CCGCTC 1 cut(s) 805
BsrFI RCCGGY 2 cut(s) 14, 62
BssAI RCCGGY 2 cut(s) 14, 62
BssECI CCNNGG 7 cut(s) 426, 519, 584, 855, 883, 903, 943
BssMI GATC 4 cut(s) 393, 814, 975, 979
BssNI GRCGYC 5 cut(s) 207, 242, 285, 381, 423
BssSI CACGAG 1 cut(s) 965
BssT1I CCWWGG 4 cut(s) 426, 584, 855, 943
Bst2BI CACGAG 1 cut(s) 965
Bst2UI CCWGG 3 cut(s) 572, 797, 956
Bst4CI ACNGT 2 cut(s) 493, 707
Bst6I CTCTTC 2 cut(s) 227, 320
BstACI GRCGYC 5 cut(s) 207, 242, 285, 381, 423
BstC8I GCNNGC 4 cut(s) 52, 64, 436, 615
BstDEI CTNAG 3 cut(s) 55, 276, 632
BstDSI CCRYGG 5 cut(s) 426, 519, 855, 883, 943
BstF5I GGATG 3 cut(s) 127, 286, 909
BstFNI CGCG 3 cut(s) 36, 239, 764
BstH2I RGCGCY 2 cut(s) 426, 506
BstHHI GCGC 4 cut(s) 38, 89, 425, 505
BstKTI GATC 4 cut(s) 396, 817, 978, 982
BstMAI GTCTC 4 cut(s) 255, 713, 786, 893
BstMBI GATC 4 cut(s) 393, 814, 975, 979
BstMCI CGRYCG 2 cut(s) 352, 982
BstMWI GCNNNNNNNGC 5 cut(s) 56, 75, 84, 431, 761
BstNI CCWGG 3 cut(s) 572, 797, 956
BstSCI CCNGG 6 cut(s) 418, 570, 774, 795, 874, 954
BstUI CGCG 3 cut(s) 36, 239, 764
BstV1I GCAGC 5 cut(s) 78, 97, 308, 370, 756
BstV2I GAAGAC 1 cut(s) 645
BstXI CCANNNNNNTGG 1 cut(s) 796
Bsu15I ATCGAT 1 cut(s) 978
Bsu36I CCTNAGG 1 cut(s) 276
BsuRI GGCC 4 cut(s) 434, 578, 755, 908
BsuTUI ATCGAT 1 cut(s) 978
BtgI CCRYGG 5 cut(s) 426, 519, 855, 883, 943
BtgZI GCGATG 3 cut(s) 17, 643, 700
BtsCI GGATG 3 cut(s) 127, 286, 909
BtsIMutI CAGTG 1 cut(s) 489
BtuMI TCGCGA 1 cut(s) 764
Cac8I GCNNGC 4 cut(s) 52, 64, 436, 615
CfoI GCGC 4 cut(s) 38, 89, 425, 505
Cfr10I RCCGGY 2 cut(s) 14, 62
Cfr13I GGNCC 3 cut(s) 433, 588, 786
ClaI ATCGAT 1 cut(s) 978
CseI GACGC 1 cut(s) 389
CsiI ACCWGGT 2 cut(s) 795, 954
Csp6I GTAC 6 cut(s) 12, 41, 328, 703, 745, 865
CviAII CATG 6 cut(s) 427, 739, 856, 868, 893, 944
CviQI GTAC 6 cut(s) 12, 41, 328, 703, 745, 865
DdeI CTNAG 3 cut(s) 55, 276, 632
DinI GGCGCC 1 cut(s) 424
DpnI GATC 4 cut(s) 395, 816, 977, 981
DpnII GATC 4 cut(s) 393, 814, 975, 979
DraIII CACNNNGTG 1 cut(s) 958
DrdI GACNNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 247
Eam1104I CTCTTC 2 cut(s) 227, 320
EarI CTCTTC 2 cut(s) 227, 320
EciI GGCGGA 3 cut(s) 190, 206, 469
Ecl136II GAGCTC 2 cut(s) 532, 986
Eco130I CCWWGG 4 cut(s) 426, 584, 855, 943
Eco147I AGGCCT 1 cut(s) 755
Eco24I GRGCYC 3 cut(s) 149, 534, 988
Eco31I GGTCTC 3 cut(s) 713, 786, 893
Eco47I GGWCC 2 cut(s) 588, 786
Eco53kI GAGCTC 2 cut(s) 532, 986
Eco81I CCTNAGG 1 cut(s) 276
Eco88I CYCGRG 2 cut(s) 142, 606
EcoICRI GAGCTC 2 cut(s) 532, 986
EcoRII CCWGG 3 cut(s) 570, 795, 954
EcoT14I CCWWGG 4 cut(s) 426, 584, 855, 943
EcoT38I GRGCYC 3 cut(s) 149, 534, 988
EgeI GGCGCC 1 cut(s) 424
EheI GGCGCC 1 cut(s) 424
ErhI CCWWGG 4 cut(s) 426, 584, 855, 943
FaeI CATG 6 cut(s) 430, 742, 859, 871, 896, 947
FaiI YATR 7 cut(s) 428, 485, 740, 857, 869, 894, 945
FaqI GGGAC 3 cut(s) 392, 566, 858
FatI CATG 6 cut(s) 426, 738, 855, 867, 892, 943
FauI CCCGC 4 cut(s) 55, 268, 443, 798
FblI GTMKAC 2 cut(s) 228, 497
FokI GGATG 3 cut(s) 114, 293, 896
FriOI GRGCYC 3 cut(s) 149, 534, 988
FspBI CTAG 1 cut(s) 663
GlaI GCGC 4 cut(s) 37, 88, 424, 504
GsaI CCCAGC 1 cut(s) 274
GsuI CTGGAG 1 cut(s) 593
HaeII RGCGCY 2 cut(s) 426, 506
HaeIII GGCC 4 cut(s) 434, 578, 755, 908
HapII CCGG 5 cut(s) 15, 63, 420, 775, 876
HgaI GACGC 1 cut(s) 389
HhaI GCGC 4 cut(s) 38, 89, 425, 505
Hin1I GRCGYC 5 cut(s) 207, 242, 285, 381, 423
Hin1II CATG 6 cut(s) 430, 742, 859, 871, 896, 947
Hin6I GCGC 4 cut(s) 36, 87, 423, 503
HinP1I GCGC 4 cut(s) 36, 87, 423, 503
HincII GTYRAC 2 cut(s) 229, 498
HindII GTYRAC 2 cut(s) 229, 498
HpaII CCGG 5 cut(s) 15, 63, 420, 775, 876
Hpy166II GTNNAC 3 cut(s) 43, 229, 498
Hpy188I TCNGA 6 cut(s) 156, 349, 529, 633, 828, 990
Hpy188III TCNNGA 8 cut(s) 131, 142, 278, 672, 763, 789, 812, 913
Hpy8I GTNNAC 3 cut(s) 43, 229, 498
Hpy99I CGWCG 8 cut(s) 191, 209, 230, 244, 290, 383, 502, 764
HpyAV CCTTC 6 cut(s) 193, 204, 521, 572, 766, 844
HpyCH4III ACNGT 2 cut(s) 493, 707
HpyCH4IV ACGT 4 cut(s) 207, 242, 285, 747
HpyCH4V TGCA 1 cut(s) 324
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 75, 84, 431, 761
HpyF3I CTNAG 3 cut(s) 55, 276, 632
HpySE526I ACGT 4 cut(s) 207, 242, 285, 747
Hsp92I GRCGYC 5 cut(s) 207, 242, 285, 381, 423
Hsp92II CATG 6 cut(s) 430, 742, 859, 871, 896, 947
HspAI GCGC 4 cut(s) 36, 87, 423, 503
KasI GGCGCC 1 cut(s) 422
KpnI GGTACC 1 cut(s) 15
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
Kzo9I GATC 4 cut(s) 393, 814, 975, 979
LmnI GCTCC 5 cut(s) 23, 110, 529, 777, 991
Lsp1109I GCAGC 5 cut(s) 78, 97, 308, 370, 756
LweI GCATC 2 cut(s) 40, 687
MabI ACCWGGT 2 cut(s) 795, 954
MaeI CTAG 1 cut(s) 663
MaeII ACGT 4 cut(s) 207, 242, 285, 747
MaeIII GTNAC 2 cut(s) 4, 721
MalI GATC 4 cut(s) 395, 816, 977, 981
MbiI CCGCTC 1 cut(s) 805
MboI GATC 4 cut(s) 393, 814, 975, 979
MboII GAAGA 4 cut(s) 214, 307, 529, 650
MhlI GDGCHC 4 cut(s) 149, 534, 615, 988
Mly113I GGCGCC 1 cut(s) 423
MlyI GAGTC 6 cut(s) 145, 362, 409, 860, 880, 888
MmeI TCCRAC 2 cut(s) 372, 475
MroNI GCCGGC 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 719
MspA1I CMGCKG 2 cut(s) 110, 321
MspI CCGG 5 cut(s) 15, 63, 420, 775, 876
MspR9I CCNGG 6 cut(s) 420, 572, 776, 797, 876, 956
MvaI CCWGG 3 cut(s) 572, 797, 956
MvnI CGCG 3 cut(s) 36, 239, 764
MwoI GCNNNNNNNGC 5 cut(s) 56, 75, 84, 431, 761
NaeI GCCGGC 1 cut(s) 64
NarI GGCGCC 1 cut(s) 423
NciI CCSGG 3 cut(s) 420, 776, 876
NcoI CCATGG 3 cut(s) 426, 855, 943
NdeII GATC 4 cut(s) 393, 814, 975, 979
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 6 cut(s) 430, 742, 859, 871, 896, 947
NlaIV GGNNCC 6 cut(s) 13, 19, 106, 424, 590, 787
NmuCI GTSAC 2 cut(s) 4, 721
NruI TCGCGA 1 cut(s) 764
PaeR7I CTCGAG 1 cut(s) 606
PceI AGGCCT 1 cut(s) 755
PcsI WCGNNNNNNNCGW 1 cut(s) 546
PdiI GCCGGC 1 cut(s) 64
PfeI GAWTC 4 cut(s) 555, 567, 644, 683
Pfl23II CGTACG 1 cut(s) 744
PflFI GACNNNGTC 1 cut(s) 797
PfoI TCCNGGA 3 cut(s) 570, 774, 874
Ple19I CGATCG 1 cut(s) 982
PleI GAGTC 6 cut(s) 145, 362, 409, 859, 879, 887
PluTI GGCGCC 1 cut(s) 426
PpsI GAGTC 6 cut(s) 145, 362, 409, 859, 879, 887
Psp124BI GAGCTC 2 cut(s) 534, 988
Psp6I CCWGG 3 cut(s) 570, 795, 954
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 3 cut(s) 570, 795, 954
PspLI CGTACG 1 cut(s) 744
PspN4I GGNNCC 6 cut(s) 13, 19, 106, 424, 590, 787
PspPI GGNCC 3 cut(s) 433, 588, 786
PspXI VCTCGAGB 1 cut(s) 606
PsrI GAACNNNNNNTAC 2 cut(s) 728, 760
PsyI GACNNNGTC 1 cut(s) 797
PvuI CGATCG 1 cut(s) 982
RruI TCGCGA 1 cut(s) 764
RsaI GTAC 6 cut(s) 13, 42, 329, 704, 746, 866
RsaNI GTAC 6 cut(s) 12, 41, 328, 703, 745, 865
RseI CAYNNNNRTG 1 cut(s) 719
SacI GAGCTC 2 cut(s) 534, 988
SalI GTCGAC 2 cut(s) 227, 496
Sau3AI GATC 4 cut(s) 393, 814, 975, 979
Sau96I GGNCC 3 cut(s) 433, 588, 786
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 6 cut(s) 145, 362, 409, 860, 880, 888
ScrFI CCNGG 6 cut(s) 420, 572, 776, 797, 876, 956
SduI GDGCHC 4 cut(s) 149, 534, 615, 988
SexAI ACCWGGT 2 cut(s) 795, 954
SfaNI GCATC 2 cut(s) 40, 687
SfoI GGCGCC 1 cut(s) 424
Sfr274I CTCGAG 1 cut(s) 606
SinI GGWCC 2 cut(s) 588, 786
SlaI CTCGAG 1 cut(s) 606
SmiMI CAYNNNNRTG 1 cut(s) 719
SmlI CTYRAG 1 cut(s) 606
SmoI CTYRAG 1 cut(s) 606
SseBI AGGCCT 1 cut(s) 755
SspDI GGCGCC 1 cut(s) 422
SspMI CTAG 1 cut(s) 663
SstI GAGCTC 2 cut(s) 534, 988
StuI AGGCCT 1 cut(s) 755
StyD4I CCNGG 6 cut(s) 418, 570, 774, 795, 874, 954
StyI CCWWGG 4 cut(s) 426, 584, 855, 943
TaaI ACNGT 2 cut(s) 493, 707
TaiI ACGT 4 cut(s) 210, 245, 288, 750
TaqII GACCGA 2 cut(s) 366, 917
TatI WGTACW 2 cut(s) 327, 864
TauI GCSGC 5 cut(s) 31, 62, 81, 92, 338
TfiI GAWTC 4 cut(s) 555, 567, 644, 683
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 2 cut(s) 4, 721
TseI GCWGC 5 cut(s) 66, 110, 321, 358, 769
Tsp45I GTSAC 2 cut(s) 4, 721
TspDTI ATGAA 1 cut(s) 615
TspGWI ACGGA 2 cut(s) 392, 872
TspRI CASTG 1 cut(s) 496
Tth111I GACNNNGTC 1 cut(s) 797
VpaK11BI GGWCC 2 cut(s) 588, 786
XhoI CTCGAG 1 cut(s) 606
XmiI GTMKAC 2 cut(s) 228, 497
XspI CTAG 1 cut(s) 663
ZraI GACGTC 3 cut(s) 208, 243, 286
ZrmI AGTACT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.