pycom10g13260

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007769
Physical Location & Seq
Reverse (-)
16708162 .. 16713385
5224 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3051 bp
ATGGAGTCGATACAAAGCAAAGTGGAGGCATGGATCAAGGAGCAGCGCGCCAAGCTCCTCAAGGTCTCTTGGGGACCACTGCAATGGAGGATGAAGTGGCCATGGCTCGGCAAGGAGGCCGACCGCCGCAGGATCCACCAGGAGTACGAGCGCCGCCGCAAGCAGCTCCACGATCTCTGCGCCGCCGTCAAGGTCGACTCCGTCTCTGACCTCCAGGACATTCTCTGCTGCATGGTCCTCTCCGAATGCGTTTACAAGAGACCTGCTTCGGACTTGGTTTGGGCTGTGAATAAGTTTAAGTCCGACTTCGGAGGACAAATTGTTTCTCTCGAGCGTGTTCAGCCCTCTTCGGATCACGTTCCGCACAGTTATTTGTTGGCGGAATCTGGTGATACATTGTTTGCTTCCTTCACTGGAACCAAGCAGTACAAGGATGTGATGACAGATGCAAACATATTTCAAGGTGCTATATTTCATGAAGATGTAGCGGAGGATACTAATGGAACTGAAACTACTAATCCACCTGAAAACCGAAAAGGAAATGGAGAACATTTGTGGAATCCTCTCGAATCAAAAGCGAATAAGGCTAAACCTGCCGCACATCGGGGGTTCTTGGCTCGTGCTAAAGGGATCCCTGCTTTGGAGTTGTACAGGCTTGCTCAAAAGAAAAACCGGAACCTTGTCTTATGTGGTCATTCACTTGGTGGAGCAGTAGCAGTGTTAGCTACCCTTGCTATTTTGAGGGTGGTTGCTGCTTCTTCTTCTTCATTAAAGGAGAACGGAAATGTTAAAGTCAAATGTATCACATTTTCCCAGCCCCCTGTTGGGAATGCAGCTTTAAGAGATTATGTTAGTAGAGAGGGATGGCAACATTACTTCAAGAGTTACTGCATTCCACAAGATCTGGTACCTCGCATCTTGTCCCCTGCTTATTTTCATCAGTATAGTGCACAATCTCTTTCAGTGCCCGCTGAAACCGAAACTACCAGCATATCCTTGTTAAAAAGTGAGGAAGCAGTAGGAAAAGGAAAAGAGAATGATGGCGAACGGTTGGTTCTGGGTTTGGGACCTGTTCAGACTTCCATGTGGAGACTCTCGAAGCTTGTTCCCTTAGAAGGCATAAGAAGACAATTCAACAAGTATAGAGGAAAGAAAGTGTATTCTGTCGAGGCATCTTCTCAGTCAGATTATGTAGCAGCAACTGTAGTAGATGATGACATGGTAGAACCACAGTCTCTTGAAATACAAGAAGGTTCTGATGGTATATCTCTTAAACCTATCTCTGACACTGATAAAGAGCCACCGTATGTGGCACCAAGTGGGAAGTCAGCTAGAAAAAGCTCCACAAAAAATGGGGATGACAGCACTTGGCGCAGAGTGCCTTATTTACCTTCATATGTACCATTCGGGGAGCTTTATCTCTTGGAAAATTCGTCAGTGAAGTCACTATCAGATGCGGAGTACTCAAATTTGACATCGGTCGGATCTGTTATTGCAGAACTGAGGGAACGATTTCAATCACATTCAATGAAGTCATATAGGTTTCGGTTCCAGAGAATCTATGACCTGTGCATGAAAGACGATACGTCACCTTTTCCAGCCATAGAGCAACTACAGCAGTTTCCACATCTACAGCAGTGGCTTGGTCTTGCAGTTGCAGGAACTGTGGAGCTTGGGCATATTGTTGAGTCTCCAGTTATTCGGACAGCTACTTCAGTTGCTCCTCTTGGATGGAATAGCATACCTGGCGAGAAGAATGGAGAGCCCTTAAAAGTTGATATCACTGGTTTTGGATTGCATCTTTGTACGCTTGTGCATGCCCAAGTGAATGGTAACTGGTGTTCAACAACAGTGGAATCCTTTCCTTCAACCCCAACTTATTCTTCAAATTATGGGGAAAAGCTGGAACTACAAAAAATGCGGGTCTTAGTTGGAGCTCCATTGAAACAGCCACCAAAATATCAGATGGAAGCAGATTCAATGATGCGTGTGTTTTCTTCTATTGATTCCAACACAGCTAATCTTAATAGGGAGCACACTTCGGGACCATTTGATGAGGAAAAGTCCATACGTTCTGAAGGCTTAAGTGAATTTTTTATATTTTGTAACAGTGACTTTACCAGAGTCTCTAAAGAGGTCCATGTGAGAACTCGCAGGGTGCGATTACTTGGTCTTGAGGGAGCCGGAAAGACTTCCCTTTTTAAGGCAATGTTATCCCAAGGCAAAATTACCAATATTACCAATATTGAAAATCTGCTTCCAGAAACTAACGCCCAAGAAGGTATTTCTCGGGGTTTATACTTCTGTGATTCAGCAGGAGTAAATTTACAGGAGCTGAATTTGGAGGCCACCCGTTTCAGAGATGAACTATGGACAGGAATTCGTGATTTGAATCAGAAGACCGACTTGATTGTTCTTGTTCATAACCTGTCTCACAGAATACCACACTTAAATCTCTCAAATGGATCGCAGCAAAAGCCAGCCCTTACACTTCTGCTGCATGAAGCAAAATCTCTGGGAATTCCTTGGGTCCTTGCCGTAACAAACAAATTTTCTGTCAGTGCTCATCAGCAGAAAGCAGCAATTGATGATGTTGTCCAGTCATACCAAGCATCTCCAAGTACCACTGCCGTTATCAATTCCTGTCCATATGTCATGCCAAGTGCTGCAAGCACTAATTTGTCATGGGGTGCAACCACTGGAGATGCTGATGGAAGGATGGGTGCTCAAAGCTTATTGTTTGCTCCCATCAATTTTGTTAATTGGCCTTTCCAGAAGAAAGAAATCATTCTCCCAGTTGAGGGTGTCAACTCTCTTCGTCAAGTAGTCCATCATGTGCTTCGTAGCCACGAGGAAGCTGGATTGCAGGAACTTGCAAGAGATAGGCTTTTGGTGGAAGTGGCACGAGAGCGTGCAATCGCCATGGGACGTGATTCTCAAGCCAAGTCAAATTCTCTTACATCTGCAGCCGTGGGTGCTTCCCTCGGAGCCGGTCTTGGCATTGTCTTGGCTGTTGTCATGGGTGCAGCATCCGCCTTGAGGAAGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

1017

Amino Acids

112.32

Weight (kDa)

8.44

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 184 - 306 4.6e-15 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 271, 601
Acc65I GGTACC 1 cut(s) 907
AccB1I GGYRCC 2 cut(s) 907, 1312
AccI GTMKAC 1 cut(s) 195
AccII CGCG 1 cut(s) 48
AclWI GGATC 8 cut(s) 41, 127, 140, 360, 625, 638, 1492, 2473
AcoI YGGCCR 1 cut(s) 98
AcsI RAATTY 9 cut(s) 1429, 1468, 2090, 2323, 2338, 2379, 2520, 2549, 2950
AcuI CTGAAG 2 cut(s) 1698, 2097
AdeI CACNNNGTG 2 cut(s) 704, 1319
AfaI GTAC 8 cut(s) 146, 428, 650, 909, 1401, 1463, 1807, 2623
AflII CTTAAG 1 cut(s) 2083
AhdI GACNNNNNGTC 1 cut(s) 1585
AjiI CACGTC 1 cut(s) 2930
AjnI CCWGG 3 cut(s) 138, 213, 1744
AjuI GAANNNNNNNTTGG 2 cut(s) 1867, 1899
Alw21I GWGCWC 5 cut(s) 952, 1939, 2037, 2566, 2728
Alw26I GTCTC 8 cut(s) 70, 208, 253, 1084, 1239, 1695, 2131, 2436
Alw44I GTGCAC 1 cut(s) 948
AlwI GGATC 8 cut(s) 41, 127, 140, 360, 625, 638, 1492, 2473
AlwNI CAGNNNCTG 3 cut(s) 1202, 1664, 2335
Ama87I CYCGRG 2 cut(s) 329, 2289
AoxI GGCC 4 cut(s) 98, 117, 2346, 2765
ApaLI GTGCAC 1 cut(s) 948
ApoI RAATTY 9 cut(s) 1429, 1468, 2090, 2323, 2338, 2379, 2520, 2549, 2950
ArsI GACNNNNNNTTYG 2 cut(s) 2541, 2573
Asp700I GAANNNNTTC 4 cut(s) 1512, 1860, 2191, 2787
Asp718I GGTACC 1 cut(s) 907
AspLEI GCGC 5 cut(s) 48, 50, 153, 182, 1374
AspS9I GGNCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
AsuHPI GGTGA 2 cut(s) 401, 1581
AvaI CYCGRG 2 cut(s) 329, 2289
AvaII GGWCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
BaeGI GKGCMC 2 cut(s) 952, 969
BalI TGGCCA 1 cut(s) 100
BamHI GGATCC 2 cut(s) 132, 630
BanI GGYRCC 2 cut(s) 907, 1312
BanII GRGCYC 2 cut(s) 1767, 1939
BauI CACGAG 3 cut(s) 618, 2849, 2904
BbsI GAAGAC 2 cut(s) 1132, 2405
Bbv12I GWGCWC 5 cut(s) 952, 1939, 2037, 2566, 2728
BccI CCATC 9 cut(s) 858, 1034, 1253, 1725, 1960, 2705, 2713, 2756, 2838
BceAI ACGGC 4 cut(s) 170, 2522, 2615, 2954
BciT130I CCWGG 3 cut(s) 140, 215, 1746
BciVI GTATCC 1 cut(s) 487
BcoDI GTCTC 8 cut(s) 70, 208, 253, 1084, 1239, 1695, 2131, 2436
BfaI CTAG 1 cut(s) 1332
BfmI CTRYAG 4 cut(s) 1203, 1613, 1631, 2964
BfoI RGCGCY 1 cut(s) 154
BfrI CTTAAG 1 cut(s) 2083
BfuAI ACCTGC 2 cut(s) 271, 601
BfuI GTATCC 1 cut(s) 487
BglII AGATCT 1 cut(s) 901
BmcAI AGTACT 1 cut(s) 1463
Bme1390I CCNGG 3 cut(s) 140, 215, 1746
Bme18I GGWCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
BmeRI GACNNNNNGTC 1 cut(s) 1585
BmeT110I CYCGRG 2 cut(s) 329, 2289
BmgBI CACGTC 1 cut(s) 2930
BmgT120I GGNCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
BmrFI CCNGG 3 cut(s) 140, 215, 1746
BmrI ACTGGG 1 cut(s) 2789
BmsI GCATC 9 cut(s) 436, 924, 1181, 1444, 1807, 1974, 2621, 2695, 3038
BmuI ACTGGG 1 cut(s) 2789
BoxI GACNNNNGTC 1 cut(s) 1478
BpiI GAAGAC 2 cut(s) 1132, 2405
BpmI CTGGAG 3 cut(s) 197, 1677, 2721
BpuEI CTTGAG 3 cut(s) 44, 2195, 2922
BsaBI GATNNNNATC 2 cut(s) 1516, 2391
BsaI GGTCTC 2 cut(s) 70, 253
BsaJI CCNNGG 6 cut(s) 101, 2218, 2525, 2922, 2970, 2983
BsaWI WCCGGW 1 cut(s) 672
Bse118I RCCGGY 1 cut(s) 2990
Bse1I ACTGG 7 cut(s) 418, 1694, 1789, 1841, 2599, 2704, 2795
Bse3DI GCAATG 2 cut(s) 89, 2214
Bse8I GATNNNNATC 2 cut(s) 1516, 2391
BseBI CCWGG 3 cut(s) 140, 215, 1746
BseDI CCNNGG 6 cut(s) 101, 2218, 2525, 2922, 2970, 2983
BseGI GGATG 7 cut(s) 96, 439, 869, 1363, 1736, 2724, 3029
BseJI GATNNNNATC 2 cut(s) 1516, 2391
BseMI GCAATG 2 cut(s) 89, 2214
BseMII CTCAG 2 cut(s) 1193, 1493
BseNI ACTGG 7 cut(s) 418, 1694, 1789, 1841, 2599, 2704, 2795
BsePI GCGCGC 1 cut(s) 46
BseRI GAGGAG 2 cut(s) 47, 1713
BseSI GKGCMC 2 cut(s) 952, 969
BseYI CCCAGC 1 cut(s) 813
BsgI GTGCAG 1 cut(s) 3045
Bsh1236I CGCG 1 cut(s) 48
Bsh1285I CGRYCG 2 cut(s) 124, 1482
BshFI GGCC 4 cut(s) 100, 119, 2348, 2767
BshNI GGYRCC 2 cut(s) 907, 1312
BsiEI CGRYCG 2 cut(s) 124, 1482
BsiHKAI GWGCWC 5 cut(s) 952, 1939, 2037, 2566, 2728
BsiHKCI CYCGRG 2 cut(s) 329, 2289
BsiSI CCGG 3 cut(s) 673, 2184, 2991
BslFI GGGAC 5 cut(s) 87, 907, 1080, 2058, 2940
BsmAI GTCTC 8 cut(s) 70, 208, 253, 1084, 1239, 1695, 2131, 2436
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 5 cut(s) 87, 907, 1080, 2058, 2940
BsmI GAATGC 3 cut(s) 251, 835, 891
BsnI GGCC 4 cut(s) 100, 119, 2348, 2767
Bso31I GGTCTC 2 cut(s) 70, 253
BsoBI CYCGRG 2 cut(s) 329, 2289
Bsp1286I GDGCHC 7 cut(s) 952, 969, 1767, 1939, 2037, 2566, 2728
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 8 cut(s) 33, 132, 172, 352, 630, 901, 1484, 2465
Bsp19I CCATGG 2 cut(s) 101, 2922
BspANI GGCC 4 cut(s) 100, 119, 2348, 2767
BspCNI CTCAG 2 cut(s) 1192, 1494
BspFNI CGCG 1 cut(s) 48
BspHI TCATGA 1 cut(s) 475
BspMAI CTGCAG 1 cut(s) 2968
BspMI ACCTGC 2 cut(s) 271, 601
BspPI GGATC 8 cut(s) 41, 127, 140, 360, 625, 638, 1492, 2473
BspT107I GGYRCC 2 cut(s) 907, 1312
BspTI CTTAAG 1 cut(s) 2083
BspTNI GGTCTC 2 cut(s) 70, 253
BsrDI GCAATG 2 cut(s) 89, 2214
BsrFI RCCGGY 1 cut(s) 2990
BsrGI TGTACA 1 cut(s) 648
BsrI ACTGG 7 cut(s) 418, 1694, 1789, 1841, 2599, 2704, 2795
BssAI RCCGGY 1 cut(s) 2990
BssECI CCNNGG 6 cut(s) 101, 2218, 2525, 2922, 2970, 2983
BssHII GCGCGC 1 cut(s) 46
BssMI GATC 8 cut(s) 33, 132, 172, 352, 630, 901, 1484, 2465
BssSI CACGAG 3 cut(s) 618, 2849, 2904
BssT1I CCWWGG 4 cut(s) 101, 2218, 2525, 2922
Bst2BI CACGAG 3 cut(s) 618, 2849, 2904
Bst2UI CCWGG 3 cut(s) 140, 215, 1746
Bst4CI ACNGT 8 cut(s) 368, 1050, 1204, 1233, 1305, 1666, 1852, 2111
Bst6I CTCTTC 2 cut(s) 352, 2820
BstAFI CTTAAG 1 cut(s) 2083
BstAUI TGTACA 1 cut(s) 648
BstC8I GCNNGC 8 cut(s) 48, 161, 657, 969, 1818, 2481, 2671, 2913
BstDEI CTNAG 5 cut(s) 1111, 1179, 1502, 1927, 3048
BstDSI CCRYGG 3 cut(s) 101, 2922, 2970
BstENI CCTNNNNNAGG 1 cut(s) 2201
BstF5I GGATG 7 cut(s) 96, 439, 869, 1363, 1736, 2724, 3029
BstFNI CGCG 1 cut(s) 48
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 5 cut(s) 48, 50, 153, 182, 1374
BstKTI GATC 8 cut(s) 36, 135, 175, 355, 633, 904, 1487, 2468
BstMAI GTCTC 8 cut(s) 70, 208, 253, 1084, 1239, 1695, 2131, 2436
BstMBI GATC 8 cut(s) 33, 132, 172, 352, 630, 901, 1484, 2465
BstMCI CGRYCG 2 cut(s) 124, 1482
BstNI CCWGG 3 cut(s) 140, 215, 1746
BstNSI RCATGY 1 cut(s) 1820
BstPAI GACNNNNGTC 1 cut(s) 1478
BstSCI CCNGG 3 cut(s) 138, 213, 1744
BstSFI CTRYAG 4 cut(s) 1203, 1613, 1631, 2964
BstSLI GKGCMC 2 cut(s) 952, 969
BstUI CGCG 1 cut(s) 48
BstV2I GAAGAC 2 cut(s) 1132, 2405
BstX2I RGATCY 4 cut(s) 132, 630, 901, 1484
BstXI CCANNNNNNTGG 2 cut(s) 84, 1829
BstYI RGATCY 4 cut(s) 132, 630, 901, 1484
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 4 cut(s) 100, 119, 2348, 2767
BtgI CCRYGG 3 cut(s) 101, 2922, 2970
BtrI CACGTC 1 cut(s) 2930
BtsCI GGATG 7 cut(s) 96, 439, 869, 1363, 1736, 2724, 3029
BtsI GCAGTG 4 cut(s) 77, 723, 1643, 2625
BveI ACCTGC 2 cut(s) 271, 601
Cac8I GCNNGC 8 cut(s) 48, 161, 657, 969, 1818, 2481, 2671, 2913
CaiI CAGNNNCTG 3 cut(s) 1202, 1664, 2335
CciI TCATGA 1 cut(s) 475
CfoI GCGC 5 cut(s) 48, 50, 153, 182, 1374
Cfr10I RCCGGY 1 cut(s) 2990
Cfr13I GGNCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
Csp6I GTAC 8 cut(s) 145, 427, 649, 908, 1400, 1462, 1806, 2622
CspCI CAANNNNNGTGG 2 cut(s) 1833, 1868
CviQI GTAC 8 cut(s) 145, 427, 649, 908, 1400, 1462, 1806, 2622
DdeI CTNAG 5 cut(s) 1111, 1179, 1502, 1927, 3048
DpnI GATC 8 cut(s) 35, 134, 174, 354, 632, 903, 1486, 2467
DpnII GATC 8 cut(s) 33, 132, 172, 352, 630, 901, 1484, 2465
DraIII CACNNNGTG 2 cut(s) 704, 1319
DriI GACNNNNNGTC 1 cut(s) 1585
EaeI YGGCCR 1 cut(s) 98
Eam1104I CTCTTC 2 cut(s) 352, 2820
Eam1105I GACNNNNNGTC 1 cut(s) 1585
EarI CTCTTC 2 cut(s) 352, 2820
EciI GGCGGA 2 cut(s) 395, 3022
Ecl136II GAGCTC 1 cut(s) 1937
Eco130I CCWWGG 4 cut(s) 101, 2218, 2525, 2922
Eco24I GRGCYC 2 cut(s) 1767, 1939
Eco31I GGTCTC 2 cut(s) 70, 253
Eco32I GATATC 1 cut(s) 1780
Eco47I GGWCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
Eco53kI GAGCTC 1 cut(s) 1937
Eco57I CTGAAG 2 cut(s) 1698, 2097
Eco88I CYCGRG 2 cut(s) 329, 2289
EcoICRI GAGCTC 1 cut(s) 1937
EcoNI CCTNNNNNAGG 1 cut(s) 2201
EcoO109I RGGNCCY 2 cut(s) 1067, 2530
EcoRI GAATTC 2 cut(s) 2379, 2520
EcoRII CCWGG 3 cut(s) 138, 213, 1744
EcoRV GATATC 1 cut(s) 1780
EcoT14I CCWWGG 4 cut(s) 101, 2218, 2525, 2922
EcoT38I GRGCYC 2 cut(s) 1767, 1939
ErhI CCWWGG 4 cut(s) 101, 2218, 2525, 2922
Esp3I CGTCTC 1 cut(s) 208
FalI AAGNNNNNCTT 4 cut(s) 290, 322, 2390, 2422
FaqI GGGAC 5 cut(s) 87, 907, 1080, 2058, 2940
FauI CCCGC 2 cut(s) 976, 1914
FauNDI CATATG 2 cut(s) 1396, 2650
FblI GTMKAC 1 cut(s) 195
FokI GGATG 7 cut(s) 103, 446, 876, 1370, 1743, 2731, 3016
FriOI GRGCYC 2 cut(s) 1767, 1939
FspBI CTAG 1 cut(s) 1332
GlaI GCGC 5 cut(s) 47, 49, 152, 181, 1373
GsaI CCCAGC 1 cut(s) 817
GsuI CTGGAG 3 cut(s) 197, 1677, 2721
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 4 cut(s) 100, 119, 2348, 2767
HapII CCGG 3 cut(s) 673, 2184, 2991
HhaI GCGC 5 cut(s) 48, 50, 153, 182, 1374
Hin6I GCGC 5 cut(s) 46, 48, 151, 180, 1372
HinP1I GCGC 5 cut(s) 46, 48, 151, 180, 1372
HincII GTYRAC 2 cut(s) 196, 2809
HindII GTYRAC 2 cut(s) 196, 2809
HindIII AAGCTT 2 cut(s) 1100, 2731
HpaII CCGG 3 cut(s) 673, 2184, 2991
HphI GGTGA 2 cut(s) 401, 1581
Hpy166II GTNNAC 4 cut(s) 196, 253, 950, 2809
Hpy8I GTNNAC 4 cut(s) 196, 253, 950, 2809
HpyAV CCTTC 8 cut(s) 418, 1109, 1244, 1401, 1875, 2072, 2273, 2709
HpyCH4III ACNGT 8 cut(s) 368, 1050, 1204, 1233, 1305, 1666, 1852, 2111
HpyCH4IV ACGT 4 cut(s) 357, 1586, 2071, 2929
HpyF3I CTNAG 5 cut(s) 1111, 1179, 1502, 1927, 3048
HpySE526I ACGT 4 cut(s) 357, 1586, 2071, 2929
HspAI GCGC 5 cut(s) 46, 48, 151, 180, 1372
KpnI GGTACC 1 cut(s) 911
Kzo9I GATC 8 cut(s) 33, 132, 172, 352, 630, 901, 1484, 2465
LweI GCATC 9 cut(s) 436, 924, 1181, 1444, 1807, 1974, 2621, 2695, 3038
MaeI CTAG 1 cut(s) 1332
MaeII ACGT 4 cut(s) 357, 1586, 2071, 2929
MaeIII GTNAC 7 cut(s) 884, 1443, 1587, 1832, 2105, 2111, 2539
MalI GATC 8 cut(s) 35, 134, 174, 354, 632, 903, 1486, 2467
MboI GATC 8 cut(s) 33, 132, 172, 352, 630, 901, 1484, 2465
MfeI CAATTG 1 cut(s) 2583
MflI RGATCY 4 cut(s) 132, 630, 901, 1484
MhlI GDGCHC 7 cut(s) 952, 969, 1767, 1939, 2037, 2566, 2728
MlsI TGGCCA 1 cut(s) 100
MluNI TGGCCA 1 cut(s) 100
MlyI GAGTC 5 cut(s) 14, 191, 1086, 1697, 2133
MmeI TCCRAC 4 cut(s) 327, 1462, 1912, 2034
Mox20I TGGCCA 1 cut(s) 100
MroXI GAANNNNTTC 4 cut(s) 1512, 1860, 2191, 2787
MscI TGGCCA 1 cut(s) 100
MslI CAYNNNNRTG 2 cut(s) 82, 480
Msp20I TGGCCA 1 cut(s) 100
MspA1I CMGCKG 1 cut(s) 971
MspCI CTTAAG 1 cut(s) 2083
MspI CCGG 3 cut(s) 673, 2184, 2991
MspR9I CCNGG 3 cut(s) 140, 215, 1746
MunI CAATTG 1 cut(s) 2583
Mva1269I GAATGC 3 cut(s) 251, 835, 891
MvaI CCWGG 3 cut(s) 140, 215, 1746
MvnI CGCG 1 cut(s) 48
NcoI CCATGG 2 cut(s) 101, 2922
NdeI CATATG 2 cut(s) 1396, 2650
NdeII GATC 8 cut(s) 33, 132, 172, 352, 630, 901, 1484, 2465
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 3 cut(s) 1443, 1587, 2111
NspI RCATGY 1 cut(s) 1820
PaeI GCATGC 1 cut(s) 1820
PaeR7I CTCGAG 1 cut(s) 329
PagI TCATGA 1 cut(s) 475
PauI GCGCGC 1 cut(s) 46
PcsI WCGNNNNNNNCGW 1 cut(s) 2158
PctI GAATGC 3 cut(s) 251, 835, 891
PdmI GAANNNNTTC 4 cut(s) 1512, 1860, 2191, 2787
PflFI GACNNNGTC 1 cut(s) 200
PfoI TCCNGGA 1 cut(s) 213
PleI GAGTC 5 cut(s) 13, 191, 1086, 1696, 2132
PpsI GAGTC 5 cut(s) 13, 191, 1086, 1696, 2132
PpuMI RGGWCCY 2 cut(s) 1067, 2530
PshAI GACNNNNGTC 1 cut(s) 1478
Psp124BI GAGCTC 1 cut(s) 1939
Psp5II RGGWCCY 2 cut(s) 1067, 2530
Psp6I CCWGG 3 cut(s) 138, 213, 1744
PspFI CCCAGC 1 cut(s) 813
PspGI CCWGG 3 cut(s) 138, 213, 1744
PspPI GGNCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
PspPPI RGGWCCY 2 cut(s) 1067, 2530
PsrI GAACNNNNNNTAC 4 cut(s) 496, 528, 1825, 1857
PstI CTGCAG 1 cut(s) 2968
PstNI CAGNNNCTG 3 cut(s) 1202, 1664, 2335
PsuI RGATCY 4 cut(s) 132, 630, 901, 1484
PsyI GACNNNGTC 1 cut(s) 200
PteI GCGCGC 1 cut(s) 46
RsaI GTAC 8 cut(s) 146, 428, 650, 909, 1401, 1463, 1807, 2623
RsaNI GTAC 8 cut(s) 145, 427, 649, 908, 1400, 1462, 1806, 2622
RseI CAYNNNNRTG 2 cut(s) 82, 480
SacI GAGCTC 1 cut(s) 1939
SalI GTCGAC 1 cut(s) 194
Sau3AI GATC 8 cut(s) 33, 132, 172, 352, 630, 901, 1484, 2465
Sau96I GGNCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
ScaI AGTACT 1 cut(s) 1463
SchI GAGTC 5 cut(s) 14, 191, 1086, 1697, 2133
ScrFI CCNGG 3 cut(s) 140, 215, 1746
SduI GDGCHC 7 cut(s) 952, 969, 1767, 1939, 2037, 2566, 2728
SfaNI GCATC 9 cut(s) 436, 924, 1181, 1444, 1807, 1974, 2621, 2695, 3038
SfcI CTRYAG 4 cut(s) 1203, 1613, 1631, 2964
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 2 cut(s) 82, 480
SmlI CTYRAG 6 cut(s) 59, 329, 2083, 2174, 2937, 3037
SmoI CTYRAG 6 cut(s) 59, 329, 2083, 2174, 2937, 3037
SphI GCATGC 1 cut(s) 1820
SspI AATATT 2 cut(s) 2236, 2245
SspMI CTAG 1 cut(s) 1332
SstI GAGCTC 1 cut(s) 1939
StyD4I CCNGG 3 cut(s) 138, 213, 1744
StyI CCWWGG 4 cut(s) 101, 2218, 2525, 2922
TaaI ACNGT 8 cut(s) 368, 1050, 1204, 1233, 1305, 1666, 1852, 2111
TaiI ACGT 4 cut(s) 360, 1589, 2074, 2932
TaqI TCGA 6 cut(s) 8, 195, 330, 567, 1097, 1167
TaqII GACCGA 2 cut(s) 1468, 2417
TatI WGTACW 3 cut(s) 426, 648, 1461
TauI GCSGC 5 cut(s) 129, 156, 159, 185, 599
TseFI GTSAC 3 cut(s) 1443, 1587, 2111
Tsp45I GTSAC 3 cut(s) 1443, 1587, 2111
TspGWI ACGGA 2 cut(s) 190, 795
Tth111I GACNNNGTC 1 cut(s) 200
Vha464I CTTAAG 1 cut(s) 2083
VneI GTGCAC 1 cut(s) 948
VpaK11BI GGWCC 6 cut(s) 74, 235, 1067, 2045, 2137, 2530
XagI CCTNNNNNAGG 1 cut(s) 2201
XapI RAATTY 9 cut(s) 1429, 1468, 2090, 2323, 2338, 2379, 2520, 2549, 2950
XceI RCATGY 1 cut(s) 1820
XcmI CCANNNNNNNNNTGG 2 cut(s) 821, 2855
XhoI CTCGAG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 195
XmnI GAANNNNTTC 4 cut(s) 1512, 1860, 2191, 2787
XspI CTAG 1 cut(s) 1332
ZrmI AGTACT 1 cut(s) 1463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.