pycom10g20520

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0017115
Physical Location & Seq
Forward (+)
23395100 .. 23396688
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1500 bp
ATGTGGCAGATGATAGATATTCTCAATTCACTCATCGGAATCATCGCTTTCTTTGGTTTTGTTCCTATACACGCAGCGGATGAGCAGAAATTCCACCCCCACCAAGAAAATATACCAATCAAAATATATACATACATACATACATACATATATATATATATATACATACATACATATATATATATATATATATATATATATATATATATATATATATGTAGCCAAACCAATCTATCAAAATCTAAAGCTTCTTTGTTCTTGGTCTCAATTGCTTCGGTAACTTCTGTTTCAATCAGTGGTGAAAACTTCTGTCAGTGCATGGCAGACTACAGCAAAATTTCTGACCAAAACATTCACACCTCAAACTCTTCTTTGTATTCATCTCTTTTGCAATCTTCCCAGCAAAACCCTAGGTGGTTAAACTCCACATTAAAGCCTCTTCTCCTTGTCACACCTTCCCATGAGTCTGAAATCCAAGCAGGTGTTCTTTGCAGCAGGAAACTTGGCCTGCAAGTCAGGGTTAGAAGTGGAGGACATGACTATGAAGGCCTATCTTACCTCTGCAAAACCCCATTTATCATCATTGACCTCATCAATCTCCATTCCCTCGATGTCAACCTTGCAGATGAAACAGCTTGGGTGCAGTCAGGAGCTACACTTGGTGAGCTTTATTATAGCATTGCCACGAAAAGTGGGGTCCATGGATTTCCAGGAGGTTTATGCCCTACTGTTGGGATTGGAGGGCACTTGAGTGGAGGTGGGTTTGGAACCCTAATCAGGAAGCATGGCCTAGCAGCTGACAATGTCATAGATGCTCGGTTAATTGATGTGAATGGAAGAATCCTTGACAGAAGAACAATGGGTGAGGATTTGTTTTGGGCCATTAGAGGAGGTGGGGGATCAAGTTTTGGGATCATAGTTTCATGGAAAATCAAATTGGTTCAGGTTCCAAAAATCGTGACAGGTTTTACAGTTGACAAAACCCTAGCAGAAGGGGTTACCAGCCTTGTTCACAGGTGGCAACATATTGCAGACAAATTTCATGAAGATCTTTTCATCAGAGTCATAATTCAGAATGTGGGGAGTGGGAATCAGAAAACGGTTCAAGCATCTTTCCAATCACTGTTTCTTGGAAAAATTGACAGATTAATCCCACTGATGAAGCTGAGCTTTCCAGAGCTGGGATTGCAGGCAAAAGACTGCATTGAGATGAGTTGGATCCAATCTGCCCAGTATTTTGCTGGATTCCAAAAAGACCAGCCCTCAGATGTCTTGCTATCAAGAAATCCCCTCTGGAAGATGAATTTCAAGGCTAAATCCGACTTTGTGAAAAACCCAATACCCGAATCAGGACTCCAAGGGATTTGGGAGAGGTTTCAGCAGGAACAAAATGTGTACATAATTTTGGATCCATATGGTGGCAAAATGAATGATCGATCTCAGAACATGAAATCCCATTTCCTCACAGAAAAGGTAATTTGTTCAACATACAGTACATAG

Protein Analysis

500

Amino Acids

56.02

Weight (kDa)

8.26

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 146 - 282 5.9e-24 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20860
fragaria_vesca FvH4_3g10830
malus_domestica MD10G1247300.v1.1
prunus_persica Prupe.4G096000_v2.0.a1 Prupe.4G096100_v2.0.a1
pyrus_communis pycom05g25170 pycom10g20520
rosa_chinensis RchiOBHm_Chr5g0017541
rosa_laevigata RLG00000032297
rosa_multiflora Rmu_sc0002565.1_g000003
rosa_roxburghii Rroxscaffold_1G00059900
rosa_rugosa Rorug05G0036800
rosa_wichuraiana Rw5G011300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 470
Acc36I ACCTGC 1 cut(s) 470
AccB7I CCANNNNNTGG 1 cut(s) 1418
AciI CCGC 1 cut(s) 77
AclWI GGATC 6 cut(s) 907, 920, 1213, 1226, 1403, 1416
AcsI RAATTY 4 cut(s) 89, 336, 1037, 1303
AdeI CACNNNGTG 1 cut(s) 662
AfaI GTAC 2 cut(s) 1397, 1495
AfiI CCNNNNNNNGG 5 cut(s) 731, 777, 1181, 1349, 1418
AgsI TTSAA 4 cut(s) 291, 1106, 1309, 1485
AjnI CCWGG 1 cut(s) 709
AjuI GAANNNNNNNTTGG 6 cut(s) 468, 500, 920, 952, 1110, 1142
AleI CACNNNNGTG 1 cut(s) 750
AloI GAACNNNNNNTCC 2 cut(s) 1436, 1468
AluBI AGCT 8 cut(s) 248, 635, 653, 667, 797, 1165, 1170, 1180
AluI AGCT 8 cut(s) 248, 635, 653, 667, 797, 1165, 1170, 1180
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 6 cut(s) 907, 920, 1213, 1226, 1403, 1416
AoxI GGCC 4 cut(s) 505, 547, 787, 879
ApeKI GCWGC 3 cut(s) 74, 492, 794
ApoI RAATTY 4 cut(s) 89, 336, 1037, 1303
AseI ATTAAT 1 cut(s) 1148
AspA2I CCTAGG 1 cut(s) 410
AspS9I GGNCC 2 cut(s) 697, 879
AsuHPI GGTGA 3 cut(s) 311, 674, 875
AvaII GGWCC 1 cut(s) 697
AvrII CCTAGG 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 747
BamHI GGATCC 2 cut(s) 1218, 1408
BbvI GCAGC 3 cut(s) 86, 504, 806
BciT130I CCWGG 1 cut(s) 711
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 3 cut(s) 411, 791, 986
BfmI CTRYAG 1 cut(s) 328
BfuAI ACCTGC 1 cut(s) 470
BglII AGATCT 1 cut(s) 1048
BisI GCNGC 3 cut(s) 75, 493, 795
BlnI CCTAGG 1 cut(s) 410
BlpI GCTNAGC 1 cut(s) 1166
BlsI GCNGC 3 cut(s) 76, 494, 796
Bme1390I CCNGG 1 cut(s) 711
Bme18I GGWCC 1 cut(s) 697
BmgT120I GGNCC 2 cut(s) 697, 879
BmiI GGNNCC 5 cut(s) 698, 769, 948, 1220, 1410
BmrFI CCNGG 1 cut(s) 711
BmrI ACTGGG 1 cut(s) 1225
BmsI GCATC 2 cut(s) 802, 1118
BmuI ACTGGG 1 cut(s) 1225
Bpu1102I GCTNAGC 1 cut(s) 1166
BpuEI CTTGAG 1 cut(s) 769
Bsa29I ATCGAT 1 cut(s) 1435
BsaI GGTCTC 1 cut(s) 268
BsaJI CCNNGG 3 cut(s) 410, 700, 1357
Bsc4I CCNNNNNNNGG 5 cut(s) 731, 777, 1181, 1349, 1418
Bse1I ACTGG 1 cut(s) 1231
Bse3DI GCAATG 1 cut(s) 678
BseBI CCWGG 1 cut(s) 711
BseCI ATCGAT 1 cut(s) 1435
BseDI CCNNGG 3 cut(s) 410, 700, 1357
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 5 cut(s) 731, 777, 1181, 1349, 1418
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 3 cut(s) 1157, 1278, 1454
BseNI ACTGG 1 cut(s) 1231
BseRI GAGGAG 1 cut(s) 903
BseSI GKGCMC 1 cut(s) 747
BseXI GCAGC 3 cut(s) 86, 504, 806
BseYI CCCAGC 2 cut(s) 399, 1180
BsgI GTGCAG 1 cut(s) 662
BshFI GGCC 4 cut(s) 507, 549, 789, 881
BshVI ATCGAT 1 cut(s) 1435
BslI CCNNNNNNNGG 5 cut(s) 731, 777, 1181, 1349, 1418
BsmAI GTCTC 1 cut(s) 268
BsnI GGCC 4 cut(s) 507, 549, 789, 881
Bso31I GGTCTC 1 cut(s) 268
Bsp1286I GDGCHC 1 cut(s) 747
Bsp1407I TGTACA 1 cut(s) 1395
Bsp143I GATC 7 cut(s) 899, 912, 1048, 1218, 1408, 1432, 1436
Bsp1720I GCTNAGC 1 cut(s) 1166
Bsp19I CCATGG 1 cut(s) 700
BspACI CCGC 1 cut(s) 77
BspANI GGCC 4 cut(s) 507, 549, 789, 881
BspCNI CTCAG 3 cut(s) 1158, 1277, 1453
BspDI ATCGAT 1 cut(s) 1435
BspHI TCATGA 1 cut(s) 1042
BspLI GGNNCC 5 cut(s) 698, 769, 948, 1220, 1410
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 6 cut(s) 907, 920, 1213, 1226, 1403, 1416
BspTNI GGTCTC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 678
BsrGI TGTACA 1 cut(s) 1395
BsrI ACTGG 1 cut(s) 1231
BssECI CCNNGG 3 cut(s) 410, 700, 1357
BssMI GATC 7 cut(s) 899, 912, 1048, 1218, 1408, 1432, 1436
BssT1I CCWWGG 3 cut(s) 410, 700, 1357
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 5 cut(s) 730, 973, 1102, 1125, 1493
Bst6I CTCTTC 2 cut(s) 373, 444
BstAUI TGTACA 1 cut(s) 1395
BstC8I GCNNGC 2 cut(s) 509, 1191
BstDEI CTNAG 3 cut(s) 1166, 1264, 1440
BstDSI CCRYGG 1 cut(s) 700
BstEII GGTNACC 1 cut(s) 997
BstF5I GGATG 1 cut(s) 85
BstKTI GATC 7 cut(s) 902, 915, 1051, 1221, 1411, 1435, 1439
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 7 cut(s) 899, 912, 1048, 1218, 1408, 1432, 1436
BstMWI GCNNNNNNNGC 1 cut(s) 1186
BstNI CCWGG 1 cut(s) 711
BstPI GGTNACC 1 cut(s) 997
BstSCI CCNGG 1 cut(s) 709
BstSFI CTRYAG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 747
BstV1I GCAGC 3 cut(s) 86, 504, 806
BstX2I RGATCY 3 cut(s) 1048, 1218, 1408
BstYI RGATCY 3 cut(s) 1048, 1218, 1408
Bsu15I ATCGAT 1 cut(s) 1435
BsuRI GGCC 4 cut(s) 507, 549, 789, 881
BsuTUI ATCGAT 1 cut(s) 1435
BtgI CCRYGG 1 cut(s) 700
BtgZI GCGATG 1 cut(s) 28
BtsCI GGATG 1 cut(s) 85
BtsIMutI CAGTG 4 cut(s) 301, 320, 1121, 1154
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 2 cut(s) 509, 1191
CciI TCATGA 1 cut(s) 1042
Cfr13I GGNCC 2 cut(s) 697, 879
ClaI ATCGAT 1 cut(s) 1435
Csp6I GTAC 2 cut(s) 1396, 1494
CviAII CATG 8 cut(s) 319, 461, 536, 701, 785, 924, 1043, 1447
CviQI GTAC 2 cut(s) 1396, 1494
DdeI CTNAG 3 cut(s) 1166, 1264, 1440
DpnI GATC 7 cut(s) 901, 914, 1050, 1220, 1410, 1434, 1438
DpnII GATC 7 cut(s) 899, 912, 1048, 1218, 1408, 1432, 1436
DraIII CACNNNGTG 1 cut(s) 662
Eam1104I CTCTTC 2 cut(s) 373, 444
EarI CTCTTC 2 cut(s) 373, 444
Eco130I CCWWGG 3 cut(s) 410, 700, 1357
Eco147I AGGCCT 1 cut(s) 549
Eco31I GGTCTC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 697
Eco91I GGTNACC 1 cut(s) 997
EcoO65I GGTNACC 1 cut(s) 997
EcoRII CCWGG 1 cut(s) 709
EcoT14I CCWWGG 3 cut(s) 410, 700, 1357
ErhI CCWWGG 3 cut(s) 410, 700, 1357
FaeI CATG 8 cut(s) 322, 464, 539, 704, 788, 927, 1046, 1450
FalI AAGNNNNNCTT 2 cut(s) 1154, 1186
FatI CATG 8 cut(s) 318, 460, 535, 700, 784, 923, 1042, 1446
FauNDI CATATG 1 cut(s) 1414
Fnu4HI GCNGC 3 cut(s) 75, 493, 795
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 3 cut(s) 75, 493, 795
FspBI CTAG 3 cut(s) 411, 791, 986
GluI GCNGC 3 cut(s) 75, 493, 795
GsaI CCCAGC 2 cut(s) 403, 1184
HaeIII GGCC 4 cut(s) 507, 549, 789, 881
Hin1II CATG 8 cut(s) 322, 464, 539, 704, 788, 927, 1046, 1450
HincII GTYRAC 2 cut(s) 616, 976
HindII GTYRAC 2 cut(s) 616, 976
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 8 cut(s) 39, 464, 840, 1062, 1090, 1245, 1346, 1353
HphI GGTGA 3 cut(s) 311, 674, 875
Hpy166II GTNNAC 4 cut(s) 616, 976, 1012, 1396
Hpy188I TCNGA 9 cut(s) 38, 343, 469, 1061, 1074, 1095, 1267, 1321, 1443
Hpy188III TCNNGA 8 cut(s) 648, 778, 958, 1043, 1175, 1281, 1294, 1350
Hpy8I GTNNAC 4 cut(s) 616, 976, 1012, 1396
HpyAV CCTTC 3 cut(s) 465, 539, 986
HpyCH4III ACNGT 5 cut(s) 730, 973, 1102, 1125, 1493
HpyF10VI GCNNNNNNNGC 1 cut(s) 1186
HpyF3I CTNAG 3 cut(s) 1166, 1264, 1440
Hsp92II CATG 8 cut(s) 322, 464, 539, 704, 788, 927, 1046, 1450
Kzo9I GATC 7 cut(s) 899, 912, 1048, 1218, 1408, 1432, 1436
LmnI GCTCC 1 cut(s) 650
Lsp1109I GCAGC 3 cut(s) 86, 504, 806
LweI GCATC 2 cut(s) 802, 1118
MaeI CTAG 3 cut(s) 411, 791, 986
MaeIII GTNAC 4 cut(s) 277, 448, 958, 997
MalI GATC 7 cut(s) 901, 914, 1050, 1220, 1410, 1434, 1438
MboI GATC 7 cut(s) 899, 912, 1048, 1218, 1408, 1432, 1436
MboII GAAGA 7 cut(s) 360, 387, 431, 849, 864, 1058, 1309
MfeI CAATTG 1 cut(s) 267
MflI RGATCY 3 cut(s) 1048, 1218, 1408
MhlI GDGCHC 1 cut(s) 747
MlyI GAGTC 3 cut(s) 473, 1071, 1347
MmeI TCCRAC 2 cut(s) 1196, 1344
MseI TTAA 4 cut(s) 419, 431, 821, 1148
MslI CAYNNNNRTG 3 cut(s) 540, 750, 1208
MspA1I CMGCKG 2 cut(s) 77, 797
MspR9I CCNGG 1 cut(s) 711
MunI CAATTG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 711
MwoI GCNNNNNNNGC 1 cut(s) 1186
NcoI CCATGG 1 cut(s) 700
NdeI CATATG 1 cut(s) 1414
NdeII GATC 7 cut(s) 899, 912, 1048, 1218, 1408, 1432, 1436
NlaIII CATG 8 cut(s) 322, 464, 539, 704, 788, 927, 1046, 1450
NlaIV GGNNCC 5 cut(s) 698, 769, 948, 1220, 1410
NmuCI GTSAC 2 cut(s) 448, 958
OliI CACNNNNGTG 1 cut(s) 750
PagI TCATGA 1 cut(s) 1042
PaqCI CACCTGC 1 cut(s) 470
PceI AGGCCT 1 cut(s) 549
PfeI GAWTC 5 cut(s) 39, 840, 1090, 1245, 1346
PflFI GACNNNGTC 1 cut(s) 803
PflMI CCANNNNNTGG 1 cut(s) 1418
PfoI TCCNGGA 1 cut(s) 709
PkrI GCNGC 3 cut(s) 76, 494, 796
PleI GAGTC 3 cut(s) 472, 1070, 1347
PpsI GAGTC 3 cut(s) 472, 1070, 1347
PshBI ATTAAT 1 cut(s) 1148
Psp6I CCWGG 1 cut(s) 709
PspEI GGTNACC 1 cut(s) 997
PspFI CCCAGC 2 cut(s) 399, 1180
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 5 cut(s) 698, 769, 948, 1220, 1410
PspPI GGNCC 2 cut(s) 697, 879
PsuI RGATCY 3 cut(s) 1048, 1218, 1408
PsyI GACNNNGTC 1 cut(s) 803
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 2 cut(s) 1397, 1495
RsaNI GTAC 2 cut(s) 1396, 1494
RseI CAYNNNNRTG 3 cut(s) 540, 750, 1208
SaqAI TTAA 4 cut(s) 419, 431, 821, 1148
SatI GCNGC 3 cut(s) 75, 493, 795
Sau3AI GATC 7 cut(s) 899, 912, 1048, 1218, 1408, 1432, 1436
Sau96I GGNCC 2 cut(s) 697, 879
SchI GAGTC 3 cut(s) 473, 1071, 1347
ScrFI CCNGG 1 cut(s) 711
SduI GDGCHC 1 cut(s) 747
SfaNI GCATC 2 cut(s) 802, 1118
SfcI CTRYAG 1 cut(s) 328
SinI GGWCC 1 cut(s) 697
SmiMI CAYNNNNRTG 3 cut(s) 540, 750, 1208
SmlI CTYRAG 1 cut(s) 748
SmoI CTYRAG 1 cut(s) 748
SseBI AGGCCT 1 cut(s) 549
SsiI CCGC 1 cut(s) 77
SspMI CTAG 3 cut(s) 411, 791, 986
StuI AGGCCT 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 709
StyI CCWWGG 3 cut(s) 410, 700, 1357
TaaI ACNGT 5 cut(s) 730, 973, 1102, 1125, 1493
TaqI TCGA 2 cut(s) 609, 1435
TatI WGTACW 2 cut(s) 1395, 1493
TfiI GAWTC 5 cut(s) 39, 840, 1090, 1245, 1346
Tru1I TTAA 4 cut(s) 419, 431, 821, 1148
Tru9I TTAA 4 cut(s) 419, 431, 821, 1148
TscAI CASTG 4 cut(s) 301, 320, 1128, 1161
TseFI GTSAC 2 cut(s) 448, 958
TseI GCWGC 3 cut(s) 74, 492, 794
Tsp45I GTSAC 2 cut(s) 448, 958
TspRI CASTG 4 cut(s) 301, 320, 1128, 1161
Tth111I GACNNNGTC 1 cut(s) 803
Van91I CCANNNNNTGG 1 cut(s) 1418
VpaK11BI GGWCC 1 cut(s) 697
VspI ATTAAT 1 cut(s) 1148
XapI RAATTY 4 cut(s) 89, 336, 1037, 1303
XcmI CCANNNNNNNNNTGG 1 cut(s) 1238
XmaJI CCTAGG 1 cut(s) 410
XspI CTAG 3 cut(s) 411, 791, 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.