pycom10g21030

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004060
Physical Location & Seq
Forward (+)
23764288 .. 23765408
1121 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 795 bp
ATGGGTTGTGTTGCATCTAGGATTGATAAGGAAGAGAGGGTGAAGGTTTGCAAGGAGAGAAAGAAGCTAATGAAACAGTTGGTAAGGTTTAGAGGTGAATTTGCTGAGGCACAATTGGCTTATTTAAGATCATTGAGGAACACTGATCGATCAACACTTAGACAATTTACCGAGTCGGAGACGTTGGAGCTTGATAATACTGCTCCATACGGCTTGGAGTCGCCAGCTTCACCACCTCCCCTTTTGCCTCCTCCTCCGCCGCCACCACCTTTGAGCCCTGATTCGAGAAAGTTGGATGATAGTCACAGAGAAGAAGTTGGTCAAGAGGAGTACATAGAACGTACTGAGGATGATAGCAGCACTCCACCACCTCCTCCAAACCTGAGCTCTTCCTCACCAGAGCATCATAAACATGATGAAACGGTGGAATTGCTTGAGGAAAAATGGGAAGAGAGTAAGATGGAATTTGAGGAAGACGAACAGGAAGAGGAAACGGTTGCTAATGTTGTGAATTCGCCGCCTAAGAATCTGCAGCTCGCAGAAGCCATCATGGAGGATGTATCAGTGAAGAAGCGGCATACTAAGGACACTGCAGATGTGCCCATGGTACTATGGAGAAGCAAGAAGACCTTGGAGGGTATAGCTAAGGAGTTAGATGATTATTTTCTGAAATCATCAGACGGTTTAAAGGAAATTGCTGTTCTTATGGATATCAAAGGAGGGGACACTTTTCTGCCACTCATTACTAGCGAAAGCAAAACATGGGGAGAGGTCCAATTCCACAAAGGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

265

Amino Acids

29.99

Weight (kDa)

4.87

Isoelectric Point (pI)

69.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 1 - 60 1.8e-21 Protein of unknown function (DUF630)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 257, 260, 518, 574
AcsI RAATTY 3 cut(s) 98, 464, 511
AfaI GTAC 3 cut(s) 332, 343, 609
AluBI AGCT 6 cut(s) 67, 190, 227, 387, 535, 644
AluI AGCT 6 cut(s) 67, 190, 227, 387, 535, 644
Alw21I GWGCWC 1 cut(s) 389
Alw26I GTCTC 1 cut(s) 173
ApeKI GCWGC 2 cut(s) 357, 532
ApoI RAATTY 3 cut(s) 98, 464, 511
AspS9I GGNCC 1 cut(s) 772
AsuHPI GGTGA 4 cut(s) 52, 107, 222, 387
AvaII GGWCC 1 cut(s) 772
BaeGI GKGCMC 1 cut(s) 603
BanII GRGCYC 2 cut(s) 278, 389
BbsI GAAGAC 2 cut(s) 480, 632
Bbv12I GWGCWC 1 cut(s) 389
BbvCI CCTCAGC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 369, 544
BccI CCATC 2 cut(s) 454, 554
BceAI ACGGC 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 2 cut(s) 18, 747
BfmI CTRYAG 2 cut(s) 530, 591
BisI GCNGC 5 cut(s) 260, 358, 518, 533, 575
BlsI GCNGC 5 cut(s) 261, 359, 519, 534, 576
Bme18I GGWCC 1 cut(s) 772
BmgT120I GGNCC 1 cut(s) 772
BmsI GCATC 2 cut(s) 23, 412
BpiI GAAGAC 2 cut(s) 480, 632
Bpu10I CCTNAGC 3 cut(s) 105, 383, 645
BpuEI CTTGAG 1 cut(s) 455
Bsa29I ATCGAT 1 cut(s) 148
BsaJI CCNNGG 2 cut(s) 603, 630
BseCI ATCGAT 1 cut(s) 148
BseDI CCNNGG 2 cut(s) 603, 630
BseGI GGATG 3 cut(s) 301, 355, 562
BseMII CTCAG 3 cut(s) 96, 336, 374
BseRI GAGGAG 4 cut(s) 240, 243, 341, 363
BseSI GKGCMC 1 cut(s) 603
BseXI GCAGC 2 cut(s) 369, 544
BshVI ATCGAT 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 389
BslFI GGGAC 1 cut(s) 737
BsmAI GTCTC 1 cut(s) 173
BsmBI CGTCTC 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 737
Bsp1286I GDGCHC 3 cut(s) 278, 389, 603
Bsp143I GATC 3 cut(s) 128, 145, 149
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 4 cut(s) 257, 260, 518, 574
BspCNI CTCAG 3 cut(s) 97, 337, 375
BspDI ATCGAT 1 cut(s) 148
BspMAI CTGCAG 2 cut(s) 534, 595
BspQI GCTCTTC 1 cut(s) 394
BssECI CCNNGG 2 cut(s) 603, 630
BssMI GATC 3 cut(s) 128, 145, 149
BssT1I CCWWGG 2 cut(s) 603, 630
Bst4CI ACNGT 4 cut(s) 78, 424, 496, 683
Bst6I CTCTTC 4 cut(s) 27, 394, 444, 480
BstC8I GCNNGC 2 cut(s) 225, 537
BstDEI CTNAG 7 cut(s) 105, 158, 345, 383, 522, 582, 645
BstDSI CCRYGG 1 cut(s) 603
BstF5I GGATG 3 cut(s) 301, 355, 562
BstKTI GATC 3 cut(s) 131, 148, 152
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 3 cut(s) 128, 145, 149
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstSFI CTRYAG 2 cut(s) 530, 591
BstSLI GKGCMC 1 cut(s) 603
BstV1I GCAGC 2 cut(s) 369, 544
BstV2I GAAGAC 2 cut(s) 480, 632
Bsu15I ATCGAT 1 cut(s) 148
BsuTUI ATCGAT 1 cut(s) 148
BtgI CCRYGG 1 cut(s) 603
BtsCI GGATG 3 cut(s) 301, 355, 562
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 3 cut(s) 141, 570, 588
Cac8I GCNNGC 2 cut(s) 225, 537
Cfr13I GGNCC 1 cut(s) 772
ClaI ATCGAT 1 cut(s) 148
Csp6I GTAC 3 cut(s) 331, 342, 608
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 4 cut(s) 413, 550, 604, 762
CviQI GTAC 3 cut(s) 331, 342, 608
DdeI CTNAG 7 cut(s) 105, 158, 345, 383, 522, 582, 645
DpnI GATC 3 cut(s) 130, 147, 151
DpnII GATC 3 cut(s) 128, 145, 149
DraI TTTAAA 1 cut(s) 687
Eam1104I CTCTTC 4 cut(s) 27, 394, 444, 480
EarI CTCTTC 4 cut(s) 27, 394, 444, 480
EciI GGCGGA 1 cut(s) 246
Ecl136II GAGCTC 1 cut(s) 387
Eco130I CCWWGG 2 cut(s) 603, 630
Eco24I GRGCYC 2 cut(s) 278, 389
Eco32I GATATC 1 cut(s) 712
Eco47I GGWCC 1 cut(s) 772
Eco53kI GAGCTC 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 387
EcoRI GAATTC 1 cut(s) 511
EcoRV GATATC 1 cut(s) 712
EcoT14I CCWWGG 2 cut(s) 603, 630
EcoT38I GRGCYC 2 cut(s) 278, 389
ErhI CCWWGG 2 cut(s) 603, 630
Esp3I CGTCTC 1 cut(s) 173
FaeI CATG 4 cut(s) 416, 553, 607, 765
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FaqI GGGAC 1 cut(s) 737
FatI CATG 4 cut(s) 412, 549, 603, 761
Fnu4HI GCNGC 5 cut(s) 260, 358, 518, 533, 575
FokI GGATG 3 cut(s) 308, 362, 569
FriOI GRGCYC 2 cut(s) 278, 389
Fsp4HI GCNGC 5 cut(s) 260, 358, 518, 533, 575
FspBI CTAG 2 cut(s) 18, 747
GluI GCNGC 5 cut(s) 260, 358, 518, 533, 575
Hin1II CATG 4 cut(s) 416, 553, 607, 765
HinfI GANTC 4 cut(s) 173, 218, 281, 526
HphI GGTGA 4 cut(s) 52, 107, 222, 387
Hpy188I TCNGA 3 cut(s) 178, 669, 679
Hpy188III TCNNGA 2 cut(s) 285, 323
HpyAV CCTTC 1 cut(s) 37
HpyCH4III ACNGT 4 cut(s) 78, 424, 496, 683
HpyCH4IV ACGT 2 cut(s) 182, 340
HpyCH4V TGCA 4 cut(s) 14, 51, 532, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 7 cut(s) 105, 158, 345, 383, 522, 582, 645
HpySE526I ACGT 2 cut(s) 182, 340
Hsp92II CATG 4 cut(s) 416, 553, 607, 765
Kzo9I GATC 3 cut(s) 128, 145, 149
LguI GCTCTTC 1 cut(s) 394
LmnI GCTCC 2 cut(s) 187, 208
LpnPI CCDG 5 cut(s) 237, 291, 395, 411, 467
Lsp1109I GCAGC 2 cut(s) 369, 544
LweI GCATC 2 cut(s) 23, 412
MaeI CTAG 2 cut(s) 18, 747
MaeII ACGT 2 cut(s) 182, 340
MaeIII GTNAC 1 cut(s) 302
MalI GATC 3 cut(s) 130, 147, 151
MboI GATC 3 cut(s) 128, 145, 149
MboII GAAGA 8 cut(s) 44, 323, 381, 461, 485, 497, 580, 637
MfeI CAATTG 1 cut(s) 113
MhlI GDGCHC 3 cut(s) 278, 389, 603
MluCI AATT 8 cut(s) 98, 113, 164, 428, 464, 511, 693, 776
MlyI GAGTC 2 cut(s) 182, 227
MmeI TCCRAC 3 cut(s) 156, 165, 273
MseI TTAA 2 cut(s) 125, 686
MslI CAYNNNNRTG 1 cut(s) 411
MunI CAATTG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 116
NcoI CCATGG 1 cut(s) 603
NdeII GATC 3 cut(s) 128, 145, 149
NlaIII CATG 4 cut(s) 416, 553, 607, 765
NmuCI GTSAC 1 cut(s) 302
PciSI GCTCTTC 1 cut(s) 394
PfeI GAWTC 2 cut(s) 281, 526
PkrI GCNGC 5 cut(s) 261, 359, 519, 534, 576
PleI GAGTC 2 cut(s) 181, 226
PpsI GAGTC 2 cut(s) 181, 226
Psp124BI GAGCTC 1 cut(s) 389
PspPI GGNCC 1 cut(s) 772
PstI CTGCAG 2 cut(s) 534, 595
RsaI GTAC 3 cut(s) 332, 343, 609
RsaNI GTAC 3 cut(s) 331, 342, 608
RseI CAYNNNNRTG 1 cut(s) 411
SacI GAGCTC 1 cut(s) 389
SapI GCTCTTC 1 cut(s) 394
SaqAI TTAA 2 cut(s) 125, 686
SatI GCNGC 5 cut(s) 260, 358, 518, 533, 575
Sau3AI GATC 3 cut(s) 128, 145, 149
Sau96I GGNCC 1 cut(s) 772
SchI GAGTC 2 cut(s) 182, 227
SduI GDGCHC 3 cut(s) 278, 389, 603
SfaNI GCATC 2 cut(s) 23, 412
SfcI CTRYAG 2 cut(s) 530, 591
SinI GGWCC 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 8 cut(s) 98, 113, 164, 428, 464, 511, 693, 776
SsiI CCGC 4 cut(s) 257, 260, 518, 574
SspMI CTAG 2 cut(s) 18, 747
SstI GAGCTC 1 cut(s) 389
StyI CCWWGG 2 cut(s) 603, 630
TaaI ACNGT 4 cut(s) 78, 424, 496, 683
TaiI ACGT 2 cut(s) 185, 343
TaqI TCGA 2 cut(s) 148, 284
TasI AATT 8 cut(s) 98, 113, 164, 428, 464, 511, 693, 776
TatI WGTACW 1 cut(s) 330
TauI GCSGC 3 cut(s) 262, 520, 577
TfiI GAWTC 2 cut(s) 281, 526
Tru1I TTAA 2 cut(s) 125, 686
Tru9I TTAA 2 cut(s) 125, 686
TscAI CASTG 3 cut(s) 148, 570, 595
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 2 cut(s) 357, 532
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 2 cut(s) 86, 432
TspRI CASTG 3 cut(s) 148, 570, 595
VpaK11BI GGWCC 1 cut(s) 772
XapI RAATTY 3 cut(s) 98, 464, 511
XspI CTAG 2 cut(s) 18, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.