Rorug05G0028200

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004060
Physical Location & Seq
Forward (+)
1808185 .. 1808773
589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0028200.1

Sequence Viewer

Length: 423 bp
ATGGAGCTCCCAAACCAAAACTTCCAGTTTTACCAATATGTCTTCCCCTCTCATCTTGTTTATCCATATCACTTGACCCCAGAAAACTATGTGCACTGGACTGAAACCCCAGAGTGTCACATTTTCTCAGCTGACCTCCCTGGTGTGAGGAAGGAGGAGATAAAAGTAGAAGTGGAGGACTCAATATACCTGATAATCAGAACACAGAGACTTGATGGTGATGGTGATGAGGAGTCCACAGAGGCTAGGAGGACCTTCACGAGGAAGTTTCGGCTTCCGGGTATGGTCGATATCGAAAGAATCTCAGCTGCATATGAAGATGGTGTGTTGACAATCACAGTGCCAAGGTCATTGAGGAGGAGTTTCTTTATTGAACCAGCTGACATGCCTGAAAGGCTTGATGTTCTTGCAAGAGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

140

Amino Acids

16.41

Weight (kDa)

4.98

Isoelectric Point (pI)

68.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 118 1.8e-17 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 261
AgsI TTSAA 1 cut(s) 374
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 5 cut(s) 7, 131, 308, 380, 416
AluI AGCT 5 cut(s) 7, 131, 308, 380, 416
Alw21I GWGCWC 2 cut(s) 9, 96
Alw26I GTCTC 1 cut(s) 202
Alw44I GTGCAC 1 cut(s) 92
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 2 cut(s) 308, 416
AspS9I GGNCC 1 cut(s) 252
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 2 cut(s) 230, 236
AvaII GGWCC 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 9
BauI CACGAG 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 9, 96
BbvI GCAGC 2 cut(s) 295, 403
BccI CCATC 3 cut(s) 209, 215, 314
BciT130I CCWGG 1 cut(s) 141
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 202
BfaI CTAG 1 cut(s) 246
BglI GCCNNNNNGGC 1 cut(s) 394
BisI GCNGC 2 cut(s) 309, 417
BlsI GCNGC 2 cut(s) 310, 418
Bme1390I CCNGG 2 cut(s) 141, 279
Bme18I GGWCC 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 252
BmrFI CCNGG 2 cut(s) 141, 279
BpiI GAAGAC 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 139, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse1I ACTGG 2 cut(s) 25, 101
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 139, 344
BseLI CCNNNNNNNGG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 141, 318
BseNI ACTGG 2 cut(s) 25, 101
BseRI GAGGAG 4 cut(s) 170, 245, 370, 373
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 2 cut(s) 295, 403
BsiHKAI GWGCWC 2 cut(s) 9, 96
BsiSI CCGG 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 202
Bsp1286I GDGCHC 2 cut(s) 9, 96
BspCNI CTCAG 2 cut(s) 140, 317
BsrI ACTGG 2 cut(s) 25, 101
BssECI CCNNGG 2 cut(s) 139, 344
BssSI CACGAG 1 cut(s) 259
BssT1I CCWWGG 1 cut(s) 344
Bst2BI CACGAG 1 cut(s) 259
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 340
BstAPI GCANNNNNTGC 1 cut(s) 416
BstDEI CTNAG 2 cut(s) 127, 304
BstENI CCTNNNNNAGG 1 cut(s) 259
BstMAI GTCTC 1 cut(s) 202
BstMWI GCNNNNNNNGC 2 cut(s) 394, 416
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 2 cut(s) 139, 277
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 295, 403
BstV2I GAAGAC 1 cut(s) 34
BtsIMutI CAGTG 2 cut(s) 94, 345
Cfr13I GGNCC 1 cut(s) 252
CviAII CATG 1 cut(s) 385
CviJI RGCY 8 cut(s) 7, 131, 245, 274, 308, 380, 397, 416
CviKI_1 RGCY 8 cut(s) 7, 131, 245, 274, 308, 380, 397, 416
DdeI CTNAG 2 cut(s) 127, 304
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 344
Eco24I GRGCYC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 139
EcoRV GATATC 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 344
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 344
FaeI CATG 1 cut(s) 388
FaiI YATR 8 cut(s) 39, 67, 90, 187, 284, 313, 315, 386
FalI AAGNNNNNCTT 1 cut(s) 403
FatI CATG 1 cut(s) 384
FauNDI CATATG 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 309, 417
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 2 cut(s) 309, 417
FspBI CTAG 1 cut(s) 246
GluI GCNGC 2 cut(s) 309, 417
HapII CCGG 1 cut(s) 278
Hin1II CATG 1 cut(s) 388
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 3 cut(s) 179, 233, 300
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 230, 236
Hpy166II GTNNAC 3 cut(s) 94, 237, 330
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 1 cut(s) 259
Hpy8I GTNNAC 3 cut(s) 94, 237, 330
HpyAV CCTTC 2 cut(s) 145, 265
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4V TGCA 3 cut(s) 94, 311, 410
HpyF10VI GCNNNNNNNGC 2 cut(s) 394, 416
HpyF3I CTNAG 2 cut(s) 127, 304
Hsp92II CATG 1 cut(s) 388
LmnI GCTCC 2 cut(s) 4, 12
Lsp1109I GCAGC 2 cut(s) 295, 403
MaeI CTAG 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 116
MboII GAAGA 2 cut(s) 34, 329
MhlI GDGCHC 2 cut(s) 9, 96
MlyI GAGTC 2 cut(s) 173, 242
MspA1I CMGCKG 3 cut(s) 131, 308, 380
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 2 cut(s) 141, 279
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 394, 416
NciI CCSGG 1 cut(s) 279
NdeI CATATG 1 cut(s) 313
NlaIII CATG 1 cut(s) 388
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 1 cut(s) 388
PfeI GAWTC 1 cut(s) 300
PkrI GCNGC 2 cut(s) 310, 418
PleI GAGTC 2 cut(s) 173, 241
PpsI GAGTC 2 cut(s) 173, 241
PpuMI RGGWCCY 1 cut(s) 252
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspPI GGNCC 1 cut(s) 252
PspPPI RGGWCCY 1 cut(s) 252
PvuII CAGCTG 3 cut(s) 131, 308, 380
SacI GAGCTC 1 cut(s) 9
SatI GCNGC 2 cut(s) 309, 417
Sau96I GGNCC 1 cut(s) 252
SchI GAGTC 2 cut(s) 173, 242
ScrFI CCNGG 2 cut(s) 141, 279
SduI GDGCHC 2 cut(s) 9, 96
SetI ASST 9 cut(s) 9, 133, 138, 192, 257, 310, 350, 382, 418
SinI GGWCC 1 cut(s) 252
SspMI CTAG 1 cut(s) 246
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 139, 277
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 1 cut(s) 340
TaqI TCGA 2 cut(s) 288, 294
TfiI GAWTC 1 cut(s) 300
TscAI CASTG 2 cut(s) 101, 345
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 2 cut(s) 308, 416
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 1 cut(s) 330
TspRI CASTG 2 cut(s) 101, 345
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 252
XagI CCTNNNNNAGG 1 cut(s) 259
XceI RCATGY 1 cut(s) 388
XspI CTAG 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.