pycom10g21560

P21-activated protein kinase-interacting protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015485
Physical Location & Seq
Reverse (-)
24140245 .. 24143257
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
ATGAGCCTAATCGCCGGCTCCTACGAGAAATTCATATGGGGCTTCAAGCTCAGACCCCTAAACCCTAGCCCCGATTCTCAATCCCTAACCCTAACCCCTCTCTTCTCCTACCCCTCCCACCTCTCCTCAATCACCTCTGTCGCCACCGCTGGTTCAGCCGCCGCGTCCGGCGGCACCGATGACACCGTCCACCTCTACGACATGTCCTCCGCCACCTCCCTCGGCTCCCTCCACGACCACTCCGCCACCGTTACCTCCCTATCCTTCTTCACCCCTCCCCACCTCTCCTTCCCCCTCAACCTCATCTCCGCCGACGCCGAGGGGTCCCTCTGCATCTACGAGGCCGACCCTTTCGTCCTCCTCAAGACCCTCAAGCCGCACAGGAAGGCGATTAACGACGTGGCGTTGCACCCGTCCGGGAAGCTGGCGTTGACGGTGGGGAAAGACGAGTGCTTGGCGATGATTAATTTGGTGAGGGGGAAGAGGAGCTTCTATTGCAGAGTGGCGAAGGAGGCAAGCTTGGTGAGCTATGATTTCTCCGGAGAGAAGTTCTATATGACTATGGAGGAGAAGGTGCTGGTTCATGAGGCTGAGGATGCTAAGTTGCTGTGTGAATTCGAGAATTCGAAGCGGGTTCTGTGCATTGAGCCCGGTGAGAATGGAATTTTATTTACCGGTGGTGAGGACCGGAGTATTACAGCGTGGGACACAAACAGTGGGAAAGTTGCATATTGCATTGAGGATGCACATCCTACCCGTGTGAAGGGAATTGTTACACTTACAAGCAAAGCTGGTGCTGAAGTCAATGAGGATCCCTATCTTGTGGCATCTGCCTCGTCAGATGGGATTATACGTGTTTGGGATGTTCGCATGGCTGTTACCGAGAAGCCAAATCCATTGGCCGAGGCTAACACAAAATCGAGGCTTACTTGTCTGGCTGGATCATCTCTTAAATCTTTCAAACGGCCACAGGTTGTAAAAAGCGTTCCAGAAGAGAAACACGATGCAGAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0022613 GO:0042254 GO:0042273 GO:0044085 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

339

Amino Acids

36.61

Weight (kDa)

5.83

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 37 - 153 5.1e-11 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 42 - 154 2e-07 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 45 - 167 1.4e-14 Prp19 WD40 domain
Beta-prop_EML PF23409 47 - 306 3.4e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 49 - 247 7.4e-16 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 49 - 203 3.5e-12 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 49 - 153 3e-06 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 54 - 179 4.6e-07 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 127 - 293 7.1e-08 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 176 - 292 8.1e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 190 - 291 4e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 194 - 291 1.8e-07 WDR5 beta-propeller domain
WD40_Prp19 PF24814 213 - 292 3.9e-06 Prp19 WD40 domain
WDR55 PF24796 216 - 291 3.1e-06 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015485)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65030
fragaria_vesca FvH4_3g05180
malus_domestica MD05G1280700.v1.1 MD10G1259100.v1.1
prunus_persica Prupe.4G084200_v2.0.a1
pyrus_communis pycom10g21560
rosa_chinensis RchiOBHm_Chr5g0014571
rosa_laevigata RLG00000032129
rosa_multiflora Rmu_co8518221.1_g000002 Rmu_sc0000120.1_g000004
rosa_roxburghii Rroxscaffold_1G00061910
rosa_rugosa Rorug05G0019500
rosa_samantha Rh5AG114700 Rh5BG111600 Rh5CG123100 Rh5DG111000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 173
AccII CGCG 1 cut(s) 164
AccIII TCCGGA 1 cut(s) 539
AciI CCGC 9 cut(s) 147, 159, 162, 171, 210, 243, 309, 377, 631
AclWI GGATC 3 cut(s) 806, 819, 949
AcoI YGGCCR 2 cut(s) 900, 965
AcsI RAATTY 4 cut(s) 29, 614, 622, 663
AcuI CTGAAG 1 cut(s) 819
AcyI GRCGYC 1 cut(s) 315
AfiI CCNNNNNNNGG 1 cut(s) 763
AflIII ACRYGT 2 cut(s) 201, 853
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 2 cut(s) 46, 961
AjiI CACGTC 1 cut(s) 400
AluBI AGCT 6 cut(s) 49, 424, 489, 519, 528, 791
AluI AGCT 6 cut(s) 49, 424, 489, 519, 528, 791
AlwI GGATC 3 cut(s) 806, 819, 949
Aor13HI TCCGGA 1 cut(s) 539
AoxI GGCC 3 cut(s) 342, 900, 965
ApoI RAATTY 4 cut(s) 29, 614, 622, 663
AseI ATTAAT 1 cut(s) 465
AsiGI ACCGGT 1 cut(s) 674
AspS9I GGNCC 2 cut(s) 324, 685
AsuC2I CCSGG 2 cut(s) 418, 651
AsuHPI GGTGA 6 cut(s) 124, 262, 484, 535, 665, 692
AsuII TTCGAA 1 cut(s) 626
AvaII GGWCC 2 cut(s) 324, 685
BamHI GGATCC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 651
BbvCI CCTCAGC 1 cut(s) 591
BccI CCATC 1 cut(s) 836
BceAI ACGGC 1 cut(s) 980
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BcnI CCSGG 2 cut(s) 418, 651
BfaI CTAG 1 cut(s) 66
BisI GCNGC 4 cut(s) 159, 162, 172, 377
BlsI GCNGC 4 cut(s) 160, 163, 173, 378
Bme1390I CCNGG 2 cut(s) 418, 651
Bme18I GGWCC 2 cut(s) 324, 685
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 2 cut(s) 324, 685
BmiI GGNNCC 6 cut(s) 19, 175, 226, 325, 326, 813
BmrFI CCNGG 2 cut(s) 418, 651
BmsI GCATC 5 cut(s) 342, 586, 733, 836, 994
Bpu10I CCTNAGC 1 cut(s) 591
Bpu14I TTCGAA 1 cut(s) 626
BpuEI CTTGAG 2 cut(s) 347, 356
BpuMI CCSGG 2 cut(s) 418, 651
BsaAI YACGTR 1 cut(s) 854
BsaBI GATNNNNATC 2 cut(s) 747, 816
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 3 cut(s) 220, 318, 903
BsaWI WCCGGW 3 cut(s) 539, 674, 687
BsaXI ACNNNNNCTCC 6 cut(s) 191, 221, 224, 254, 290, 320
Bsc4I CCNNNNNNNGG 1 cut(s) 763
Bse118I RCCGGY 2 cut(s) 14, 674
Bse8I GATNNNNATC 2 cut(s) 747, 816
BseAI TCCGGA 1 cut(s) 539
BseDI CCNNGG 3 cut(s) 220, 318, 903
BseGI GGATG 4 cut(s) 601, 748, 748, 868
BseJI GATNNNNATC 2 cut(s) 747, 816
BseLI CCNNNNNNNGG 1 cut(s) 763
BseMII CTCAG 2 cut(s) 64, 582
BseRI GAGGAG 4 cut(s) 115, 350, 499, 581
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 3 cut(s) 344, 902, 967
BshNI GGYRCC 1 cut(s) 173
BshTI ACCGGT 1 cut(s) 674
BsiSI CCGG 7 cut(s) 15, 168, 417, 540, 651, 675, 688
BslFI GGGAC 2 cut(s) 310, 719
BslI CCNNNNNNNGG 1 cut(s) 763
BsmFI GGGAC 2 cut(s) 310, 719
BsnI GGCC 3 cut(s) 344, 902, 967
Bsp119I TTCGAA 1 cut(s) 626
Bsp1286I GDGCHC 1 cut(s) 651
Bsp13I TCCGGA 1 cut(s) 539
Bsp143I GATC 2 cut(s) 811, 941
BspACI CCGC 9 cut(s) 147, 159, 162, 171, 210, 243, 309, 377, 631
BspANI GGCC 3 cut(s) 344, 902, 967
BspCNI CTCAG 2 cut(s) 63, 583
BspEI TCCGGA 1 cut(s) 539
BspFNI CGCG 1 cut(s) 164
BspHI TCATGA 1 cut(s) 583
BspLI GGNNCC 6 cut(s) 19, 175, 226, 325, 326, 813
BspPI GGATC 3 cut(s) 806, 819, 949
BspT104I TTCGAA 1 cut(s) 626
BspT107I GGYRCC 1 cut(s) 173
BsrFI RCCGGY 2 cut(s) 14, 674
BssAI RCCGGY 2 cut(s) 14, 674
BssECI CCNNGG 3 cut(s) 220, 318, 903
BssMI GATC 2 cut(s) 811, 941
BssNI GRCGYC 1 cut(s) 315
Bst4CI ACNGT 4 cut(s) 187, 250, 436, 716
Bst6I CTCTTC 3 cut(s) 107, 476, 987
BstACI GRCGYC 1 cut(s) 315
BstBAI YACGTR 1 cut(s) 854
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 3 cut(s) 16, 426, 517
BstDEI CTNAG 3 cut(s) 50, 591, 600
BstF5I GGATG 4 cut(s) 601, 748, 748, 868
BstFNI CGCG 1 cut(s) 164
BstKTI GATC 2 cut(s) 814, 944
BstMBI GATC 2 cut(s) 811, 941
BstMWI GCNNNNNNNGC 5 cut(s) 155, 495, 512, 525, 596
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 2 cut(s) 416, 649
BstUI CGCG 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
BsuRI GGCC 3 cut(s) 344, 902, 967
BtgZI GCGATG 1 cut(s) 473
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 4 cut(s) 601, 748, 748, 868
BtsIMutI CAGTG 1 cut(s) 721
Cac8I GCNNGC 3 cut(s) 16, 426, 517
CciI TCATGA 1 cut(s) 583
Cfr10I RCCGGY 2 cut(s) 14, 674
Cfr13I GGNCC 2 cut(s) 324, 685
CseI GACGC 2 cut(s) 153, 323
CspAI ACCGGT 1 cut(s) 674
CviAII CATG 3 cut(s) 202, 584, 871
DdeI CTNAG 3 cut(s) 50, 591, 600
DpnI GATC 2 cut(s) 813, 943
DpnII GATC 2 cut(s) 811, 941
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 2 cut(s) 900, 965
Eam1104I CTCTTC 3 cut(s) 107, 476, 987
EarI CTCTTC 3 cut(s) 107, 476, 987
EciI GGCGGA 3 cut(s) 199, 232, 298
Eco24I GRGCYC 1 cut(s) 651
Eco47I GGWCC 2 cut(s) 324, 685
Eco57I CTGAAG 1 cut(s) 819
EcoO109I RGGNCCY 1 cut(s) 324
EcoRI GAATTC 2 cut(s) 614, 622
EcoT38I GRGCYC 1 cut(s) 651
FaeI CATG 3 cut(s) 205, 587, 874
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 2 cut(s) 310, 719
FatI CATG 3 cut(s) 201, 583, 870
FauI CCCGC 1 cut(s) 624
FauNDI CATATG 1 cut(s) 35
Fnu4HI GCNGC 4 cut(s) 159, 162, 172, 377
FokI GGATG 4 cut(s) 608, 735, 755, 875
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 4 cut(s) 159, 162, 172, 377
FspBI CTAG 1 cut(s) 66
GluI GCNGC 4 cut(s) 159, 162, 172, 377
HaeIII GGCC 3 cut(s) 344, 902, 967
HapII CCGG 7 cut(s) 15, 168, 417, 540, 651, 675, 688
HgaI GACGC 2 cut(s) 153, 323
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 3 cut(s) 205, 587, 874
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HindIII AAGCTT 1 cut(s) 517
HinfI GANTC 1 cut(s) 74
HpaII CCGG 7 cut(s) 15, 168, 417, 540, 651, 675, 688
HphI GGTGA 6 cut(s) 124, 262, 484, 535, 665, 692
Hpy166II GTNNAC 2 cut(s) 190, 432
Hpy188I TCNGA 2 cut(s) 53, 841
Hpy188III TCNNGA 5 cut(s) 364, 540, 584, 619, 989
Hpy8I GTNNAC 2 cut(s) 190, 432
Hpy99I CGWCG 2 cut(s) 317, 401
HpyAV CCTTC 6 cut(s) 274, 298, 379, 502, 565, 757
HpyCH4III ACNGT 4 cut(s) 187, 250, 436, 716
HpyCH4IV ACGT 2 cut(s) 399, 853
HpyCH4V TGCA 8 cut(s) 333, 409, 498, 642, 728, 735, 746, 1007
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 495, 512, 525, 596
HpyF3I CTNAG 3 cut(s) 50, 591, 600
HpySE526I ACGT 2 cut(s) 399, 853
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 3 cut(s) 205, 587, 874
KflI GGGWCCC 1 cut(s) 324
Kpn2I TCCGGA 1 cut(s) 539
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Kzo9I GATC 2 cut(s) 811, 941
LmnI GCTCC 3 cut(s) 23, 230, 486
LweI GCATC 5 cut(s) 342, 586, 733, 836, 994
MaeI CTAG 1 cut(s) 66
MaeII ACGT 2 cut(s) 399, 853
MaeIII GTNAC 3 cut(s) 250, 772, 877
MalI GATC 2 cut(s) 813, 943
MboI GATC 2 cut(s) 811, 941
MboII GAAGA 4 cut(s) 94, 259, 493, 1004
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 6 cut(s) 29, 466, 614, 622, 663, 768
MroI TCCGGA 1 cut(s) 539
MroNI GCCGGC 1 cut(s) 14
MseI TTAA 3 cut(s) 393, 465, 951
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 7 cut(s) 15, 168, 417, 540, 651, 675, 688
MspR9I CCNGG 2 cut(s) 418, 651
MvnI CGCG 1 cut(s) 164
MwoI GCNNNNNNNGC 5 cut(s) 155, 495, 512, 525, 596
NaeI GCCGGC 1 cut(s) 16
NciI CCSGG 2 cut(s) 418, 651
NdeI CATATG 1 cut(s) 35
NdeII GATC 2 cut(s) 811, 941
NgoMIV GCCGGC 1 cut(s) 14
NlaIII CATG 3 cut(s) 205, 587, 874
NlaIV GGNNCC 6 cut(s) 19, 175, 226, 325, 326, 813
NmeAIII GCCGAG 3 cut(s) 201, 343, 928
NspI RCATGY 1 cut(s) 205
NspV TTCGAA 1 cut(s) 626
PagI TCATGA 1 cut(s) 583
PciI ACATGT 1 cut(s) 201
PdiI GCCGGC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 74
PflFI GACNNNGTC 1 cut(s) 185
PfoI TCCNGGA 1 cut(s) 416
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 4 cut(s) 160, 163, 173, 378
Ppu21I YACGTR 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 324
PscI ACATGT 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 324
PspN4I GGNNCC 6 cut(s) 19, 175, 226, 325, 326, 813
PspPI GGNCC 2 cut(s) 324, 685
PspPPI RGGWCCY 1 cut(s) 324
PsrI GAACNNNNNNTAC 2 cut(s) 969, 1001
PsuI RGATCY 1 cut(s) 811
PsyI GACNNNGTC 1 cut(s) 185
SaqAI TTAA 3 cut(s) 393, 465, 951
SatI GCNGC 4 cut(s) 159, 162, 172, 377
Sau3AI GATC 2 cut(s) 811, 941
Sau96I GGNCC 2 cut(s) 324, 685
ScrFI CCNGG 2 cut(s) 418, 651
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 5 cut(s) 342, 586, 733, 836, 994
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 2 cut(s) 324, 685
SmlI CTYRAG 2 cut(s) 362, 371
SmoI CTYRAG 2 cut(s) 362, 371
Sse9I AATT 6 cut(s) 29, 466, 614, 622, 663, 768
SsiI CCGC 9 cut(s) 147, 159, 162, 171, 210, 243, 309, 377, 631
SspMI CTAG 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 416, 649
TaaI ACNGT 4 cut(s) 187, 250, 436, 716
TaiI ACGT 2 cut(s) 402, 856
TaqI TCGA 3 cut(s) 618, 626, 920
TasI AATT 6 cut(s) 29, 466, 614, 622, 663, 768
TauI GCSGC 4 cut(s) 161, 164, 174, 379
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 3 cut(s) 393, 465, 951
Tru9I TTAA 3 cut(s) 393, 465, 951
TscAI CASTG 1 cut(s) 721
TspDTI ATGAA 2 cut(s) 22, 572
TspRI CASTG 1 cut(s) 721
Tth111I GACNNNGTC 1 cut(s) 185
VpaK11BI GGWCC 2 cut(s) 324, 685
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 4 cut(s) 29, 614, 622, 663
XceI RCATGY 1 cut(s) 205
XspI CTAG 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.