pycom10g21770

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011177
Physical Location & Seq
Reverse (-)
24329665 .. 24337562
7898 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2682 bp
ATGCATATAAAAAAGAAAAAAAATAAAAATGAATTAACACACACTCTCTCCCTCTTCTCTTTCTCTCTCTATCTCTGTCTCGTATCGGGCTCTAATCTATCTCAGCAGCTGCCTTCTCAAATCTTCGTCTCGCTTTCTCTCTCCTCAGTTTCTCTCTCTATCTCTTTCACTTACAATGCGCTGTTGATGAGTTTGATGTCTCACCCCAACAAACGCTCCTCAGCGATCAACCACAGCGGCACCTCCTCCTCCTCCTCTCTCAACCCTTCTTCCGCCGGCCCACCAATGAAAAAGGCCAAGTCGCAGGCGGTGGCCTGTTCCCTCGATCCCAGCAAAAATGGCCTCCACCACCATCACCACCACCACCCTCACACTCACCCGTCCCAGGACCCCGATAACGACGCCGTATTCGACCCCTCTTCCATGTCCCTCGACGAAGATCTCAAGCCCGACGACCCCTCTCCCCGAGGCATCGCCGCCAATTTATCCCGCAAAAAGGCGCAGCCCCCTCAGCCCTCTACCAAGAAGCTTGTCATCAAGCTCGTCAAAGCTAAACCCACGTTACCCACGAATTTTGAAGAGGAAACATGGGCAAAGCTGAAATCAGCTATTTCTGCTATATTCTTAAAGAAACCTGATTCTTGTGACTTGGAGAAGCTTTATCAGGCTGTTAATGATCTCTGCCTGCACAAGATGGGAGGAAGTCTTTATCAACGAATTGAGAAGGAGTGTGAAAGACACATAGCTGCAGCGCTGCAATCTTTGGTTGGCCAAAGCCCAGATTTAGTGGTTTTTTTATCACTTGTTGAGAGATGCTGGCAGGACCTTTGTGACCAGATGTTAATGATTCGCGGTATAGCCTTATATCTTGATAGGACATACGTGAAACAAACTCCAAATGTGCGTTCGTTGTGGGACATGGGGTTGCAGCTTTTCCGCAAACACCTTTCTCTGTCTCCAGAAGTTGAGCACAAAACTGTCACTGGCCTTTTAAGAATGATCGAGAAAGAAAGATTAGGTGAAGCAGTGGCTAGGACTCTGCTTAACCATCTTTTGAAGATGTTTACAGCTTTAGGAATCTACTCAGAAAGCTTTGAGAAGCCATTTCTTGAGTGTACTTCTGAATTTTATGCTGCTGAAGGCATGAAATATATGCAGCAAGCAGATGTTCCGGATTACTTGAAGCATGTGGAGACAAGGTTGCATGAAGAACATGAAAGATGTTTAATCTACCTGGATGCAAGTACACGAAAGCCTTTAGTTGCAACAGCGGAAAAACAGCTTTTCGATCGCCATATACCAGCCATTCTTGACAAGGGTTTCACATTGCTGATGGATGGGAATCGCATTGAGGACCTTCAAAGGATGCACACTCTTTTTTCCAGAGTCAATGCCCTTGAATCACTAAGGCAAGCGCTTAGCTCATACATCCGGAGGACAGGGCAGGGCATTATCATGGATGAAGAGAAAGATAAAGAAATGGTTGCTTCTCTTTTGGAATTTAAGGCTTCTCTTGACACTATATGGGAAGAAAGCTTTTTCAAGAATGAAGTATTCTGCAACACCATAAAGGATGCCTTCGAGCATTTAATTAATCTCCGACAGAACCGACCTGCTGAGCTGATAGCAAAGTTTTTGGACGAGAAGCTTCGTGCTGGGAATAAGGGTACCTCTGAAGAGGAATTGGAGGGAATGCTTGACAAAGTCTTGGTTTTATTCAGGTTTATACAGGGTAAGGACGTGTTTGAAGCATTTTATAAGAACGATCTTGCAAAAAGGCTTCTTTTGGGAAAGAGTGCTTCTATTGACGCAGAGAAGTCTATGATTTCCAAGCTGAAGACCGAGTGTGGCAGTCAGTTTACAAACAAACTCGAAGGAATGTTTAAGGATATTGAACTCTCAAAAGAGATAAACGAGTCCTTCAGACAATCATCCCAGGCCAGGACAAAACTCCCATCAGGGATTGAGATGAGTGTTCATGTCCTTACAACTGGGTACTGGCCAACTTATCCTCCCATGGATGTTAGACTTCCTCATGAATTGAATGTATACCAGGATATCTTCAAGGAGTTTTACCTAAGCAAGTACAGTGGTAGGCGTTTAATGTGGCAAAATTCATTAGGTCACTGTGTCTTGAAAGCAGATTTTCCTAAAGGCAAAAAGGAGCTTGCAGTTTCTCTGTTTCAGACTGTTGTCTTGATGCTGTTTAATGACGCTGAGAAGCTAAGCCTTGAAGACATTAAGGATTCAACTGGCATTGAGGATAAGGAACTCAGAAGAACTCTGCAGTCCCTTGCATGTGGAAAAGTTCGTGTCCTACAAAAGTTTCCCAAAGGGAGAGACGTGGATGATGGTGATACATTTACATTCAATGACACTTTCATTGCTCCTCTCTACCGCATAAAGGTGAATGCGATCCAAATGAAGGAAACAGTGGAGGAGAACACGAGCACCACTGAAAGAGTATTCCAAGACCGCCAATATCAGGTTGATGCTGCTATTGTTCGGATAATGAAAACTAGGAAGGTGCTGAGTCACACTCTTCTGATAACAGAACTGTTCCAACAGCTTAAATTTCCGATAAAGCCGGCGGATTTGAAGAAAAGGATTGAAAGCCTGATTGACCGGGAGTATCTAGAACGGGACAAGAACAACCCTCAAATTTACAACTACCTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000209 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009987 GO:0010033 GO:0010099 GO:0010100 GO:0010154 GO:0010182 GO:0010228 GO:0016567 GO:0019538 GO:0019899 GO:0022414 GO:0023052 GO:0031461 GO:0031625 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048519 GO:0048572 GO:0048573 GO:0048575 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0061458 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071322 GO:0071495 GO:0071704 GO:0080008 GO:0090304 GO:0090567 GO:0099402 GO:1901360 GO:1901564 GO:1901700 GO:1901701 GO:1902494 GO:1990234 GO:2000026 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko03420 ko04120 map03420 map04120
Pfam Domains
Protein Families
Cullin Cullin_Nedd8

Protein Analysis

894

Amino Acids

101.97

Weight (kDa)

8.49

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 197 - 638 1.6e-153 Cullin alpha solenoid domain
Cullin_AB PF26557 661 - 794 4.8e-48 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 825 - 885 2.7e-24 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46210
fragaria_vesca FvH4_3g05480 FvH4_3g05480 FvH4_3g05480
malus_domestica MD05G1283200.v1.1 MD10G1261500.v1.1
prunus_persica Prupe.4G081400_v2.0.a1
pyrus_communis pycom05g26250 pycom10g21770
rosa_chinensis RchiOBHm_Chr5g0014271
rosa_laevigata RLG00000032095
rosa_multiflora Rmu_co8222354.1_g000001 Rmu_sc0009295.1_g000011
rosa_roxburghii Rroxscaffold_1G00062300
rosa_rugosa Rorug05G0016700
rosa_samantha Rh5AG111100 Rh5BG108300 Rh5CG119900 Rh5DG106900
rosa_wichuraiana Rw5G009690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1758
Acc36I ACCTGC 1 cut(s) 1621
Acc65I GGTACC 1 cut(s) 1667
AccB1I GGYRCC 2 cut(s) 239, 1667
AccI GTMKAC 1 cut(s) 2049
AccII CGCG 1 cut(s) 852
AccIII TCCGGA 2 cut(s) 1171, 1431
AclWI GGATC 2 cut(s) 320, 2410
AcoI YGGCCR 2 cut(s) 769, 2000
AcsI RAATTY 6 cut(s) 571, 1124, 1499, 2113, 2573, 2661
AcuI CTGAAG 4 cut(s) 1158, 1695, 1856, 1906
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 5 cut(s) 1117, 1246, 1669, 1997, 2087
AfeI AGCGCT 2 cut(s) 753, 1416
AfiI CCNNNNNNNGG 6 cut(s) 385, 496, 1941, 2404, 2425, 2485
AflIII ACRYGT 1 cut(s) 1740
AjiI CACGTC 2 cut(s) 1741, 2344
AjnI CCWGG 5 cut(s) 384, 1233, 1935, 1940, 2052
AjuI GAANNNNNNNTTGG 2 cut(s) 889, 921
Alw21I GWGCWC 2 cut(s) 972, 2453
Alw26I GTCTC 6 cut(s) 83, 133, 204, 960, 1187, 2334
AlwI GGATC 2 cut(s) 320, 2410
AlwNI CAGNNNCTG 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 465
Aor13HI TCCGGA 2 cut(s) 1171, 1431
Aor51HI AGCGCT 2 cut(s) 753, 1416
AoxI GGCC 8 cut(s) 277, 294, 312, 340, 769, 985, 1938, 2000
ApoI RAATTY 6 cut(s) 571, 1124, 1499, 2113, 2573, 2661
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AseI ATTAAT 1 cut(s) 1593
Asp718I GGTACC 1 cut(s) 1667
AspLEI GCGC 4 cut(s) 181, 502, 754, 1417
AspS9I GGNCC 4 cut(s) 278, 388, 823, 1354
AsuC2I CCSGG 1 cut(s) 2627
AsuHPI GGTGA 6 cut(s) 194, 347, 368, 1031, 2366, 2419
AvaI CYCGRG 1 cut(s) 465
AvaII GGWCC 3 cut(s) 388, 823, 1354
BalI TGGCCA 2 cut(s) 771, 2002
BanI GGYRCC 2 cut(s) 239, 1667
BanII GRGCYC 1 cut(s) 92
BauI CACGAG 1 cut(s) 2446
BbsI GAAGAC 2 cut(s) 1844, 2241
Bbv12I GWGCWC 2 cut(s) 972, 2453
BbvCI CCTCAGC 2 cut(s) 220, 510
BccI CCATC 7 cut(s) 360, 688, 1056, 1327, 1331, 1963, 2345
BceAI ACGGC 1 cut(s) 389
BciT130I CCWGG 5 cut(s) 386, 1235, 1937, 1942, 2054
BcnI CCSGG 1 cut(s) 2627
BcoDI GTCTC 6 cut(s) 83, 133, 204, 960, 1187, 2334
BfaI CTAG 4 cut(s) 1032, 2520, 2636, 2675
BfmI CTRYAG 2 cut(s) 747, 2285
BfoI RGCGCY 2 cut(s) 755, 1418
BfuAI ACCTGC 1 cut(s) 1621
BglII AGATCT 1 cut(s) 439
BlpI GCTNAGC 3 cut(s) 1418, 1617, 2225
Bme1390I CCNGG 6 cut(s) 386, 1235, 1937, 1942, 2054, 2627
Bme18I GGWCC 3 cut(s) 388, 823, 1354
BmeT110I CYCGRG 1 cut(s) 465
BmgBI CACGTC 2 cut(s) 1741, 2344
BmgT120I GGNCC 4 cut(s) 278, 388, 823, 1354
BmiI GGNNCC 3 cut(s) 241, 390, 1669
BmrFI CCNGG 6 cut(s) 386, 1235, 1937, 1942, 2054, 2627
BmrI ACTGGG 1 cut(s) 2001
BmsI GCATC 7 cut(s) 480, 803, 1228, 1356, 1564, 2190, 2482
BmuI ACTGGG 1 cut(s) 2001
BoxI GACNNNNGTC 1 cut(s) 2192
BpiI GAAGAC 2 cut(s) 1844, 2241
BplI GAGNNNNNCTC 2 cut(s) 2524, 2556
BpmI CTGGAG 1 cut(s) 942
Bpu10I CCTNAGC 3 cut(s) 220, 510, 2078
Bpu1102I GCTNAGC 3 cut(s) 1418, 1617, 2225
BpuEI CTTGAG 2 cut(s) 428, 1130
BpuMI CCSGG 1 cut(s) 2627
BsaAI YACGTR 1 cut(s) 883
BsaBI GATNNNNATC 1 cut(s) 1341
BsaHI GRCGYC 1 cut(s) 402
BsaJI CCNNGG 4 cut(s) 384, 466, 1935, 2016
BsaWI WCCGGW 2 cut(s) 1171, 1431
BsaXI ACNNNNNCTCC 8 cut(s) 32, 62, 200, 230, 1184, 1214, 1996, 2026
Bsc4I CCNNNNNNNGG 6 cut(s) 385, 496, 1941, 2404, 2425, 2485
Bse118I RCCGGY 2 cut(s) 275, 2587
Bse1I ACTGG 4 cut(s) 988, 1996, 2003, 2257
Bse3DI GCAATG 2 cut(s) 1325, 2382
Bse8I GATNNNNATC 1 cut(s) 1341
BseAI TCCGGA 2 cut(s) 1171, 1431
BseBI CCWGG 5 cut(s) 386, 1235, 1937, 1942, 2054
BseDI CCNNGG 4 cut(s) 384, 466, 1935, 2016
BseGI GGATG 9 cut(s) 1243, 1342, 1371, 1428, 1465, 1579, 1931, 2026, 2353
BseJI GATNNNNATC 1 cut(s) 1341
BseLI CCNNNNNNNGG 6 cut(s) 385, 496, 1941, 2404, 2425, 2485
BseMI GCAATG 2 cut(s) 1325, 2382
BseMII CTCAG 9 cut(s) 116, 159, 234, 524, 1098, 1608, 2208, 2287, 2522
BseNI ACTGG 4 cut(s) 988, 1996, 2003, 2257
BseRI GAGGAG 8 cut(s) 133, 208, 235, 238, 241, 244, 2379, 2453
BseYI CCCAGC 2 cut(s) 329, 1655
BsgI GTGCAG 1 cut(s) 671
Bsh1236I CGCG 1 cut(s) 852
Bsh1285I CGRYCG 1 cut(s) 1291
BshFI GGCC 8 cut(s) 279, 296, 314, 342, 771, 987, 1940, 2002
BshNI GGYRCC 2 cut(s) 239, 1667
BsiEI CGRYCG 1 cut(s) 1291
BsiHKAI GWGCWC 2 cut(s) 972, 2453
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 5 cut(s) 276, 1172, 1432, 2588, 2626
BslFI GGGAC 5 cut(s) 367, 412, 929, 2275, 2657
BslI CCNNNNNNNGG 6 cut(s) 385, 496, 1941, 2404, 2425, 2485
BsmAI GTCTC 6 cut(s) 83, 133, 204, 960, 1187, 2334
BsmBI CGTCTC 2 cut(s) 133, 2334
BsmFI GGGAC 5 cut(s) 367, 412, 929, 2275, 2657
BsmI GAATGC 2 cut(s) 1698, 2416
BsnI GGCC 8 cut(s) 279, 296, 314, 342, 771, 987, 1940, 2002
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 3 cut(s) 92, 972, 2453
Bsp13I TCCGGA 2 cut(s) 1171, 1431
Bsp143I GATC 8 cut(s) 225, 325, 439, 676, 999, 1288, 1765, 2415
Bsp1720I GCTNAGC 3 cut(s) 1418, 1617, 2225
Bsp19I CCATGG 1 cut(s) 2016
BspANI GGCC 8 cut(s) 279, 296, 314, 342, 771, 987, 1940, 2002
BspCNI CTCAG 9 cut(s) 115, 158, 233, 523, 1097, 1609, 2209, 2286, 2523
BspEI TCCGGA 2 cut(s) 1171, 1431
BspFNI CGCG 1 cut(s) 852
BspHI TCATGA 1 cut(s) 2035
BspLI GGNNCC 3 cut(s) 241, 390, 1669
BspMAI CTGCAG 2 cut(s) 751, 2289
BspMI ACCTGC 1 cut(s) 1621
BspPI GGATC 2 cut(s) 320, 2410
BspT107I GGYRCC 2 cut(s) 239, 1667
BsrDI GCAATG 2 cut(s) 1325, 2382
BsrFI RCCGGY 2 cut(s) 275, 2587
BsrI ACTGG 4 cut(s) 988, 1996, 2003, 2257
BssAI RCCGGY 2 cut(s) 275, 2587
BssECI CCNNGG 4 cut(s) 384, 466, 1935, 2016
BssMI GATC 8 cut(s) 225, 325, 439, 676, 999, 1288, 1765, 2415
BssNAI GTATAC 1 cut(s) 2050
BssNI GRCGYC 1 cut(s) 402
BssSI CACGAG 1 cut(s) 2446
BssT1I CCWWGG 1 cut(s) 2016
Bst1107I GTATAC 1 cut(s) 2050
Bst2BI CACGAG 1 cut(s) 2446
Bst2UI CCWGG 5 cut(s) 386, 1235, 1937, 1942, 2054
Bst4CI ACNGT 6 cut(s) 979, 2090, 2129, 2191, 2434, 2559
Bst6I CTCTTC 6 cut(s) 59, 424, 573, 1458, 1671, 2547
BstACI GRCGYC 1 cut(s) 402
BstBAI YACGTR 1 cut(s) 883
BstC8I GCNNGC 8 cut(s) 277, 306, 686, 818, 1161, 1413, 2169, 2589
BstDSI CCRYGG 1 cut(s) 2016
BstF5I GGATG 9 cut(s) 1243, 1342, 1371, 1428, 1465, 1579, 1931, 2026, 2353
BstFNI CGCG 1 cut(s) 852
BstH2I RGCGCY 2 cut(s) 755, 1418
BstHHI GCGC 4 cut(s) 181, 502, 754, 1417
BstKTI GATC 8 cut(s) 228, 328, 442, 679, 1002, 1291, 1768, 2418
BstMAI GTCTC 6 cut(s) 83, 133, 204, 960, 1187, 2334
BstMBI GATC 8 cut(s) 225, 325, 439, 676, 999, 1288, 1765, 2415
BstMCI CGRYCG 1 cut(s) 1291
BstMWI GCNNNNNNNGC 3 cut(s) 339, 511, 614
BstNI CCWGG 5 cut(s) 386, 1235, 1937, 1942, 2054
BstNSI RCATGY 2 cut(s) 1190, 2301
BstPAI GACNNNNGTC 1 cut(s) 2192
BstSCI CCNGG 6 cut(s) 384, 1233, 1935, 1940, 2052, 2625
BstSFI CTRYAG 2 cut(s) 747, 2285
BstUI CGCG 1 cut(s) 852
BstV2I GAAGAC 2 cut(s) 1844, 2241
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BstZ17I GTATAC 1 cut(s) 2050
BsuRI GGCC 8 cut(s) 279, 296, 314, 342, 771, 987, 1940, 2002
BtgI CCRYGG 1 cut(s) 2016
BtgZI GCGATG 1 cut(s) 457
BtrI CACGTC 2 cut(s) 1741, 2344
BtsCI GGATG 9 cut(s) 1243, 1342, 1371, 1428, 1465, 1579, 1931, 2026, 2353
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 6 cut(s) 981, 1032, 2095, 2125, 2439, 2454
BveI ACCTGC 1 cut(s) 1621
Cac8I GCNNGC 8 cut(s) 277, 306, 686, 818, 1161, 1413, 2169, 2589
CaiI CAGNNNCTG 1 cut(s) 109
CciI TCATGA 1 cut(s) 2035
CfoI GCGC 4 cut(s) 181, 502, 754, 1417
Cfr10I RCCGGY 2 cut(s) 275, 2587
Cfr13I GGNCC 4 cut(s) 278, 388, 823, 1354
CseI GACGC 3 cut(s) 410, 1817, 2222
Csp6I GTAC 5 cut(s) 1116, 1245, 1668, 1996, 2086
CspCI CAANNNNNGTGG 4 cut(s) 556, 591, 2071, 2106
CviQI GTAC 5 cut(s) 1116, 1245, 1668, 1996, 2086
DpnI GATC 8 cut(s) 227, 327, 441, 678, 1001, 1290, 1767, 2417
DpnII GATC 8 cut(s) 225, 325, 439, 676, 999, 1288, 1765, 2415
EaeI YGGCCR 2 cut(s) 769, 2000
Eam1104I CTCTTC 6 cut(s) 59, 424, 573, 1458, 1671, 2547
EarI CTCTTC 6 cut(s) 59, 424, 573, 1458, 1671, 2547
EciI GGCGGA 2 cut(s) 262, 2606
Eco130I CCWWGG 1 cut(s) 2016
Eco24I GRGCYC 1 cut(s) 92
Eco32I GATATC 1 cut(s) 2059
Eco47I GGWCC 3 cut(s) 388, 823, 1354
Eco47III AGCGCT 2 cut(s) 753, 1416
Eco57I CTGAAG 4 cut(s) 1158, 1695, 1856, 1906
Eco88I CYCGRG 1 cut(s) 465
EcoO109I RGGNCCY 3 cut(s) 388, 823, 1354
EcoRII CCWGG 5 cut(s) 384, 1233, 1935, 1940, 2052
EcoRV GATATC 1 cut(s) 2059
EcoT14I CCWWGG 1 cut(s) 2016
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 2016
Esp3I CGTCTC 2 cut(s) 133, 2334
FalI AAGNNNNNCTT 2 cut(s) 1562, 1594
FaqI GGGAC 5 cut(s) 367, 412, 929, 2275, 2657
FauI CCCGC 1 cut(s) 497
FblI GTMKAC 1 cut(s) 2049
FokI GGATG 9 cut(s) 1250, 1349, 1378, 1415, 1472, 1586, 1918, 2033, 2360
FriOI GRGCYC 1 cut(s) 92
FspBI CTAG 4 cut(s) 1032, 2520, 2636, 2675
GlaI GCGC 4 cut(s) 180, 501, 753, 1416
GsaI CCCAGC 2 cut(s) 333, 1659
GsuI CTGGAG 1 cut(s) 942
HaeII RGCGCY 2 cut(s) 755, 1418
HaeIII GGCC 8 cut(s) 279, 296, 314, 342, 771, 987, 1940, 2002
HapII CCGG 5 cut(s) 276, 1172, 1432, 2588, 2626
HgaI GACGC 3 cut(s) 410, 1817, 2222
HhaI GCGC 4 cut(s) 181, 502, 754, 1417
Hin1I GRCGYC 1 cut(s) 402
Hin6I GCGC 4 cut(s) 179, 500, 752, 1415
HinP1I GCGC 4 cut(s) 179, 500, 752, 1415
HindIII AAGCTT 5 cut(s) 527, 656, 1090, 1534, 1646
HpaII CCGG 5 cut(s) 276, 1172, 1432, 2588, 2626
HphI GGTGA 6 cut(s) 194, 347, 368, 1031, 2366, 2419
Hpy166II GTNNAC 5 cut(s) 1065, 1116, 1247, 1860, 2050
Hpy8I GTNNAC 5 cut(s) 1065, 1116, 1247, 1860, 2050
Hpy99I CGWCG 3 cut(s) 404, 437, 455
HpyCH4III ACNGT 6 cut(s) 979, 2090, 2129, 2191, 2434, 2559
HpyCH4IV ACGT 4 cut(s) 560, 882, 1740, 2343
HpyF10VI GCNNNNNNNGC 3 cut(s) 339, 511, 614
HpySE526I ACGT 4 cut(s) 560, 882, 1740, 2343
Hsp92I GRCGYC 1 cut(s) 402
HspAI GCGC 4 cut(s) 179, 500, 752, 1415
Kpn2I TCCGGA 2 cut(s) 1171, 1431
KpnI GGTACC 1 cut(s) 1671
KroI GCCGGC 2 cut(s) 275, 2587
KroNI GCCGGC 2 cut(s) 277, 2589
Kzo9I GATC 8 cut(s) 225, 325, 439, 676, 999, 1288, 1765, 2415
LmnI GCTCC 3 cut(s) 221, 2164, 2392
LweI GCATC 7 cut(s) 480, 803, 1228, 1356, 1564, 2190, 2482
MaeI CTAG 4 cut(s) 1032, 2520, 2636, 2675
MaeII ACGT 4 cut(s) 560, 882, 1740, 2343
MaeIII GTNAC 6 cut(s) 561, 644, 830, 979, 2123, 2534
MalI GATC 8 cut(s) 227, 327, 441, 678, 1001, 1290, 1767, 2417
MboI GATC 8 cut(s) 225, 325, 439, 676, 999, 1288, 1765, 2415
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 3 cut(s) 92, 972, 2453
MlsI TGGCCA 2 cut(s) 771, 2002
MluNI TGGCCA 2 cut(s) 771, 2002
MlyI GAGTC 4 cut(s) 1030, 1395, 1925, 2542
MmeI TCCRAC 2 cut(s) 1624, 2587
Mox20I TGGCCA 2 cut(s) 771, 2002
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 2 cut(s) 1171, 1431
MroNI GCCGGC 2 cut(s) 275, 2587
MscI TGGCCA 2 cut(s) 771, 2002
MslI CAYNNNNRTG 2 cut(s) 1454, 2405
Msp20I TGGCCA 2 cut(s) 771, 2002
MspA1I CMGCKG 3 cut(s) 109, 237, 1271
MspI CCGG 5 cut(s) 276, 1172, 1432, 2588, 2626
MspR9I CCNGG 6 cut(s) 386, 1235, 1937, 1942, 2054, 2627
Mva1269I GAATGC 2 cut(s) 1698, 2416
MvaI CCWGG 5 cut(s) 386, 1235, 1937, 1942, 2054
MvnI CGCG 1 cut(s) 852
MwoI GCNNNNNNNGC 3 cut(s) 339, 511, 614
NaeI GCCGGC 2 cut(s) 277, 2589
NciI CCSGG 1 cut(s) 2627
NcoI CCATGG 1 cut(s) 2016
NdeII GATC 8 cut(s) 225, 325, 439, 676, 999, 1288, 1765, 2415
NgoMIV GCCGGC 2 cut(s) 275, 2587
NlaIV GGNNCC 3 cut(s) 241, 390, 1669
NmuCI GTSAC 5 cut(s) 644, 830, 979, 2123, 2534
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 1190, 2301
PacI TTAATTAA 1 cut(s) 1593
PagI TCATGA 1 cut(s) 2035
PcsI WCGNNNNNNNCGW 2 cut(s) 408, 566
PctI GAATGC 2 cut(s) 1698, 2416
PdiI GCCGGC 2 cut(s) 277, 2589
PfeI GAWTC 6 cut(s) 638, 847, 1077, 1342, 1400, 2246
PflFI GACNNNGTC 1 cut(s) 1703
Ple19I CGATCG 1 cut(s) 1291
PleI GAGTC 4 cut(s) 1030, 1394, 1924, 2541
PpsI GAGTC 4 cut(s) 1030, 1394, 1924, 2541
Ppu21I YACGTR 1 cut(s) 883
PpuMI RGGWCCY 3 cut(s) 388, 823, 1354
PshAI GACNNNNGTC 1 cut(s) 2192
PshBI ATTAAT 1 cut(s) 1593
PsiI TTATAA 1 cut(s) 1758
Psp5II RGGWCCY 3 cut(s) 388, 823, 1354
Psp6I CCWGG 5 cut(s) 384, 1233, 1935, 1940, 2052
PspFI CCCAGC 2 cut(s) 329, 1655
PspGI CCWGG 5 cut(s) 384, 1233, 1935, 1940, 2052
PspN4I GGNNCC 3 cut(s) 241, 390, 1669
PspPI GGNCC 4 cut(s) 278, 388, 823, 1354
PspPPI RGGWCCY 3 cut(s) 388, 823, 1354
PstI CTGCAG 2 cut(s) 751, 2289
PstNI CAGNNNCTG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 1703
PvuI CGATCG 1 cut(s) 1291
PvuII CAGCTG 1 cut(s) 109
RsaI GTAC 5 cut(s) 1117, 1246, 1669, 1997, 2087
RsaNI GTAC 5 cut(s) 1116, 1245, 1668, 1996, 2086
RseI CAYNNNNRTG 2 cut(s) 1454, 2405
Sau3AI GATC 8 cut(s) 225, 325, 439, 676, 999, 1288, 1765, 2415
Sau96I GGNCC 4 cut(s) 278, 388, 823, 1354
SchI GAGTC 4 cut(s) 1030, 1395, 1925, 2542
ScrFI CCNGG 6 cut(s) 386, 1235, 1937, 1942, 2054, 2627
SduI GDGCHC 3 cut(s) 92, 972, 2453
SfaNI GCATC 7 cut(s) 480, 803, 1228, 1356, 1564, 2190, 2482
SfcI CTRYAG 2 cut(s) 747, 2285
SinI GGWCC 3 cut(s) 388, 823, 1354
SmiMI CAYNNNNRTG 2 cut(s) 1454, 2405
SmlI CTYRAG 2 cut(s) 443, 1109
SmoI CTYRAG 2 cut(s) 443, 1109
SspMI CTAG 4 cut(s) 1032, 2520, 2636, 2675
StyD4I CCNGG 6 cut(s) 384, 1233, 1935, 1940, 2052, 2625
StyI CCWWGG 1 cut(s) 2016
TaaI ACNGT 6 cut(s) 979, 2090, 2129, 2191, 2434, 2559
TaiI ACGT 4 cut(s) 563, 885, 1743, 2346
TaqI TCGA 7 cut(s) 324, 411, 432, 1002, 1287, 1581, 1872
TaqII GACCGA 1 cut(s) 1856
TatI WGTACW 3 cut(s) 1115, 1244, 2085
TauI GCSGC 2 cut(s) 240, 479
TfiI GAWTC 6 cut(s) 638, 847, 1077, 1342, 1400, 2246
TscAI CASTG 6 cut(s) 988, 1032, 2095, 2132, 2439, 2461
TseFI GTSAC 5 cut(s) 644, 830, 979, 2123, 2534
Tsp45I GTSAC 5 cut(s) 644, 830, 979, 2123, 2534
TspRI CASTG 6 cut(s) 988, 1032, 2095, 2132, 2439, 2461
Tth111I GACNNNGTC 1 cut(s) 1703
VpaK11BI GGWCC 3 cut(s) 388, 823, 1354
VspI ATTAAT 1 cut(s) 1593
XapI RAATTY 6 cut(s) 571, 1124, 1499, 2113, 2573, 2661
XbaI TCTAGA 1 cut(s) 2635
XceI RCATGY 2 cut(s) 1190, 2301
XmiI GTMKAC 1 cut(s) 2049
XspI CTAG 4 cut(s) 1032, 2520, 2636, 2675
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.