pycom10g22000

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0016780
Physical Location & Seq
Forward (+)
24523873 .. 24525730
1858 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGGCGGAGGAGGAGGAGCCGAAGAAGAGAAGGATGGTGGTGGAATCCCTCGGATGGCTGACGGAGTCCTCAATTATGCCGAAGAAGCACCGCGCCATCGCCGGCGTTGGAGCCTCCTCAATCATGGAGCTCAAGGCCCAACTCTATCAGTCCCAAGAAGAATCCAAGAAGTCCAAAGAACTCGCCGGCTCAGACATCGAGTTCCATCGGGCCAAGAAGAGAATTACCGCACACGACGACTTCTCCGCCAAAAACTCCGGCGTCGACGCCCGGGCCCACAAGGACAAGCTTGAGCTAAAAGCAGTGAAGGATGGATCGGCGAGCTATGCAGCGTTGGAGAAGAAGGCTGCATTGTACGATAAGTTAGCAAGGGGTGAGCTATCGGATGAAGAAGATAAAGAAAAGTATTGTGTGGATTTTTACGGCAAGAGAGTTGAGCATGACGAACCGCAGCAGACTCAGCACCGTGATTCACCTGCTGTAATATCTCCGGAGAATCAAGATGGAGAGAGCAATGCTTCTACGCTGTTTAGCACGAAACCTTTGGGGCTTGGGCGAGCAGATGCAACAGTGGACAATGATATTCACAAGCGTTTTGTGAGGGAAGTACACGAAGAAGCAAATCAAGCGAGAGAGAAGGCATCTGAGCTCAAACTGCGCAGGGAAGAGCAAGCAGCTGCTCGTCGTGAGAAGTTAAAACAGGCTTATATACGGAAACAGTTAGAGAGACTTAAAGCAGCATCAAGTAATAAGGAACAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

255

Amino Acids

28.71

Weight (kDa)

8.39

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016780)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05790
malus_domestica MD10G1263800.v1.1
prunus_persica Prupe.4G078700_v2.0.a1
pyrus_communis pycom05g26480 pycom10g22000
rosa_chinensis RchiOBHm_Chr5g0013711
rosa_laevigata RLG00000032042
rosa_multiflora Rmu_sc0002573.1_g000001
rosa_roxburghii Rroxscaffold_1G00062900
rosa_rugosa Rorug05G0012500
rosa_samantha Rh5AG106800 Rh5BG102900 Rh5CG114800 Rh5DG101600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 484
Acc16I TGCGCA 1 cut(s) 659
Acc36I ACCTGC 1 cut(s) 484
AccI GTMKAC 1 cut(s) 264
AccII CGCG 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 490
AciI CCGC 5 cut(s) 5, 91, 228, 246, 449
AclWI GGATC 1 cut(s) 322
AcyI GRCGYC 2 cut(s) 261, 267
AfaI GTAC 2 cut(s) 356, 609
AfiI CCNNNNNNNGG 1 cut(s) 54
AluBI AGCT 7 cut(s) 130, 289, 295, 324, 379, 649, 677
AluI AGCT 7 cut(s) 130, 289, 295, 324, 379, 649, 677
Alw21I GWGCWC 2 cut(s) 132, 651
Alw26I GTCTC 1 cut(s) 721
AlwI GGATC 1 cut(s) 322
Ama87I CYCGRG 1 cut(s) 270
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 3 cut(s) 135, 210, 273
ApaI GGGCCC 1 cut(s) 277
ApeKI GCWGC 6 cut(s) 329, 347, 451, 674, 677, 737
AspLEI GCGC 2 cut(s) 95, 660
AspS9I GGNCC 4 cut(s) 136, 210, 273, 274
AsuC2I CCSGG 2 cut(s) 271, 272
AsuHPI GGTGA 2 cut(s) 386, 465
AvaI CYCGRG 1 cut(s) 270
BaeGI GKGCMC 1 cut(s) 277
BanII GRGCYC 3 cut(s) 132, 277, 651
Bbv12I GWGCWC 2 cut(s) 132, 651
BbvI GCAGC 6 cut(s) 334, 341, 463, 664, 686, 749
BccI CCATC 6 cut(s) 28, 48, 104, 213, 305, 497
BceAI ACGGC 1 cut(s) 439
BcnI CCSGG 2 cut(s) 271, 272
BcoDI GTCTC 1 cut(s) 721
BfuAI ACCTGC 1 cut(s) 484
BisI GCNGC 6 cut(s) 330, 348, 452, 675, 678, 738
BlsI GCNGC 6 cut(s) 331, 349, 453, 676, 679, 739
Bme1390I CCNGG 2 cut(s) 271, 272
BmeT110I CYCGRG 1 cut(s) 270
BmgT120I GGNCC 4 cut(s) 136, 210, 273, 274
BmiI GGNNCC 3 cut(s) 18, 112, 275
BmrFI CCNGG 2 cut(s) 271, 272
BmsI GCATC 3 cut(s) 553, 650, 749
BpuEI CTTGAG 2 cut(s) 116, 311
BpuMI CCSGG 2 cut(s) 271, 272
BsaHI GRCGYC 2 cut(s) 261, 267
BsaJI CCNNGG 2 cut(s) 49, 270
BsaWI WCCGGW 1 cut(s) 490
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse118I RCCGGY 2 cut(s) 101, 185
Bse3DI GCAATG 1 cut(s) 520
BseAI TCCGGA 1 cut(s) 490
BseDI CCNNGG 2 cut(s) 49, 270
BseGI GGATG 4 cut(s) 39, 59, 316, 391
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 520
BseMII CTCAG 3 cut(s) 204, 473, 636
BseRI GAGGAG 4 cut(s) 23, 26, 29, 106
BseSI GKGCMC 1 cut(s) 277
BseXI GCAGC 6 cut(s) 334, 341, 463, 664, 686, 749
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 3 cut(s) 137, 212, 275
BsiHKAI GWGCWC 2 cut(s) 132, 651
BsiHKCI CYCGRG 1 cut(s) 270
BsiSI CCGG 5 cut(s) 102, 186, 258, 271, 491
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 721
BsmFI GGGAC 1 cut(s) 136
BsnI GGCC 3 cut(s) 137, 212, 275
BsoBI CYCGRG 1 cut(s) 270
Bsp120I GGGCCC 1 cut(s) 273
Bsp1286I GDGCHC 3 cut(s) 132, 277, 651
Bsp13I TCCGGA 1 cut(s) 490
Bsp143I GATC 1 cut(s) 314
BspACI CCGC 5 cut(s) 5, 91, 228, 246, 449
BspANI GGCC 3 cut(s) 137, 212, 275
BspCNI CTCAG 3 cut(s) 203, 472, 637
BspEI TCCGGA 1 cut(s) 490
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 18, 112, 275
BspMI ACCTGC 1 cut(s) 484
BspPI GGATC 1 cut(s) 322
BspQI GCTCTTC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 520
BsrFI RCCGGY 2 cut(s) 101, 185
BssAI RCCGGY 2 cut(s) 101, 185
BssECI CCNNGG 2 cut(s) 49, 270
BssMI GATC 1 cut(s) 314
BssNI GRCGYC 2 cut(s) 261, 267
Bst4CI ACNGT 3 cut(s) 467, 571, 720
Bst6I CTCTTC 3 cut(s) 20, 212, 660
BstACI GRCGYC 2 cut(s) 261, 267
BstC8I GCNNGC 5 cut(s) 103, 187, 322, 558, 672
BstDEI CTNAG 3 cut(s) 190, 459, 645
BstF5I GGATG 4 cut(s) 39, 59, 316, 391
BstFNI CGCG 1 cut(s) 93
BstHHI GCGC 2 cut(s) 95, 660
BstKTI GATC 1 cut(s) 317
BstMAI GTCTC 1 cut(s) 721
BstMBI GATC 1 cut(s) 314
BstMWI GCNNNNNNNGC 5 cut(s) 85, 326, 460, 626, 655
BstSCI CCNGG 2 cut(s) 269, 270
BstSLI GKGCMC 1 cut(s) 277
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 6 cut(s) 334, 341, 463, 664, 686, 749
BsuRI GGCC 3 cut(s) 137, 212, 275
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 4 cut(s) 39, 59, 316, 391
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 2 cut(s) 309, 576
BveI ACCTGC 1 cut(s) 484
Cac8I GCNNGC 5 cut(s) 103, 187, 322, 558, 672
CfoI GCGC 2 cut(s) 95, 660
Cfr10I RCCGGY 2 cut(s) 101, 185
Cfr13I GGNCC 4 cut(s) 136, 210, 273, 274
Cfr9I CCCGGG 1 cut(s) 270
CseI GACGC 2 cut(s) 250, 275
Csp6I GTAC 2 cut(s) 355, 608
CviAII CATG 3 cut(s) 124, 440, 762
CviQI GTAC 2 cut(s) 355, 608
DdeI CTNAG 3 cut(s) 190, 459, 645
DpnI GATC 1 cut(s) 316
DpnII GATC 1 cut(s) 314
Eam1104I CTCTTC 3 cut(s) 20, 212, 660
EarI CTCTTC 3 cut(s) 20, 212, 660
EciI GGCGGA 2 cut(s) 20, 235
Ecl136II GAGCTC 2 cut(s) 130, 649
Eco24I GRGCYC 3 cut(s) 132, 277, 651
Eco53kI GAGCTC 2 cut(s) 130, 649
Eco88I CYCGRG 1 cut(s) 270
EcoICRI GAGCTC 2 cut(s) 130, 649
EcoT38I GRGCYC 3 cut(s) 132, 277, 651
FaeI CATG 3 cut(s) 127, 443, 765
FaiI YATR 7 cut(s) 77, 125, 327, 441, 708, 710, 763
FaqI GGGAC 1 cut(s) 136
FatI CATG 3 cut(s) 123, 439, 761
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 6 cut(s) 330, 348, 452, 675, 678, 738
FokI GGATG 4 cut(s) 46, 66, 323, 398
FriOI GRGCYC 3 cut(s) 132, 277, 651
Fsp4HI GCNGC 6 cut(s) 330, 348, 452, 675, 678, 738
FspI TGCGCA 1 cut(s) 659
GlaI GCGC 2 cut(s) 94, 659
GluI GCNGC 6 cut(s) 330, 348, 452, 675, 678, 738
HaeIII GGCC 3 cut(s) 137, 212, 275
HapII CCGG 5 cut(s) 102, 186, 258, 271, 491
HgaI GACGC 2 cut(s) 250, 275
HhaI GCGC 2 cut(s) 95, 660
Hin1I GRCGYC 2 cut(s) 261, 267
Hin1II CATG 3 cut(s) 127, 443, 765
Hin6I GCGC 2 cut(s) 93, 658
HinP1I GCGC 2 cut(s) 93, 658
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 6 cut(s) 44, 65, 161, 457, 470, 496
HpaII CCGG 5 cut(s) 102, 186, 258, 271, 491
HphI GGTGA 2 cut(s) 386, 465
Hpy166II GTNNAC 3 cut(s) 265, 574, 610
Hpy188I TCNGA 4 cut(s) 53, 193, 385, 646
Hpy188III TCNNGA 3 cut(s) 491, 500, 686
Hpy8I GTNNAC 3 cut(s) 265, 574, 610
Hpy99I CGWCG 4 cut(s) 239, 266, 269, 687
HpyAV CCTTC 4 cut(s) 24, 301, 337, 631
HpyCH4III ACNGT 3 cut(s) 467, 571, 720
HpyCH4V TGCA 3 cut(s) 329, 350, 566
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 326, 460, 626, 655
HpyF3I CTNAG 3 cut(s) 190, 459, 645
Hsp92I GRCGYC 2 cut(s) 261, 267
Hsp92II CATG 3 cut(s) 127, 443, 765
HspAI GCGC 2 cut(s) 93, 658
Kpn2I TCCGGA 1 cut(s) 490
KroI GCCGGC 2 cut(s) 101, 185
KroNI GCCGGC 2 cut(s) 103, 187
Kzo9I GATC 1 cut(s) 314
LguI GCTCTTC 1 cut(s) 660
LmnI GCTCC 3 cut(s) 16, 110, 127
LpnPI CCDG 8 cut(s) 115, 199, 271, 284, 489, 504, 646, 686
Lsp1109I GCAGC 6 cut(s) 334, 341, 463, 664, 686, 749
LweI GCATC 3 cut(s) 553, 650, 749
MalI GATC 1 cut(s) 316
MboI GATC 1 cut(s) 314
MhlI GDGCHC 3 cut(s) 132, 277, 651
MluCI AATT 2 cut(s) 72, 222
MlyI GAGTC 2 cut(s) 74, 451
MmeI TCCRAC 2 cut(s) 88, 315
MnlI CCTC 7 cut(s) 4, 7, 59, 79, 124, 127, 594
MreI CGCCGGCG 1 cut(s) 101
MroI TCCGGA 1 cut(s) 490
MroNI GCCGGC 2 cut(s) 101, 185
MseI TTAA 2 cut(s) 695, 732
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 5 cut(s) 102, 186, 258, 271, 491
MspR9I CCNGG 2 cut(s) 271, 272
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 5 cut(s) 85, 326, 460, 626, 655
NaeI GCCGGC 2 cut(s) 103, 187
NciI CCSGG 2 cut(s) 271, 272
NdeII GATC 1 cut(s) 314
NgoMIV GCCGGC 2 cut(s) 101, 185
NlaIII CATG 3 cut(s) 127, 443, 765
NlaIV GGNNCC 3 cut(s) 18, 112, 275
NsbI TGCGCA 1 cut(s) 659
PaqCI CACCTGC 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 660
PdiI GCCGGC 2 cut(s) 103, 187
PfeI GAWTC 4 cut(s) 44, 161, 470, 496
PflFI GACNNNGTC 1 cut(s) 64
PkrI GCNGC 6 cut(s) 331, 349, 453, 676, 679, 739
PleI GAGTC 2 cut(s) 73, 451
PpsI GAGTC 2 cut(s) 73, 451
Psp124BI GAGCTC 2 cut(s) 132, 651
PspN4I GGNNCC 3 cut(s) 18, 112, 275
PspOMI GGGCCC 1 cut(s) 273
PspPI GGNCC 4 cut(s) 136, 210, 273, 274
PsyI GACNNNGTC 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 2 cut(s) 356, 609
RsaNI GTAC 2 cut(s) 355, 608
SacI GAGCTC 2 cut(s) 132, 651
SalI GTCGAC 1 cut(s) 263
SapI GCTCTTC 1 cut(s) 660
SaqAI TTAA 2 cut(s) 695, 732
SatI GCNGC 6 cut(s) 330, 348, 452, 675, 678, 738
Sau3AI GATC 1 cut(s) 314
Sau96I GGNCC 4 cut(s) 136, 210, 273, 274
SchI GAGTC 2 cut(s) 74, 451
ScrFI CCNGG 2 cut(s) 271, 272
SduI GDGCHC 3 cut(s) 132, 277, 651
SetI ASST 9 cut(s) 132, 291, 297, 326, 381, 478, 544, 651, 679
SfaNI GCATC 3 cut(s) 553, 650, 749
SgrAI CRCCGGYG 1 cut(s) 101
SgrDI CGTCGACG 1 cut(s) 263
SmaI CCCGGG 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 131, 290
SmoI CTYRAG 2 cut(s) 131, 290
SrfI GCCCGGGC 1 cut(s) 272
Sse9I AATT 2 cut(s) 72, 222
SsiI CCGC 5 cut(s) 5, 91, 228, 246, 449
SstI GAGCTC 2 cut(s) 132, 651
StyD4I CCNGG 2 cut(s) 269, 270
TaaI ACNGT 3 cut(s) 467, 571, 720
TaqI TCGA 2 cut(s) 198, 264
TasI AATT 2 cut(s) 72, 222
TatI WGTACW 1 cut(s) 607
TfiI GAWTC 4 cut(s) 44, 161, 470, 496
Tru1I TTAA 2 cut(s) 695, 732
Tru9I TTAA 2 cut(s) 695, 732
TscAI CASTG 2 cut(s) 309, 576
TseI GCWGC 6 cut(s) 329, 347, 451, 674, 677, 737
TspDTI ATGAA 1 cut(s) 402
TspGWI ACGGA 2 cut(s) 77, 727
TspMI CCCGGG 1 cut(s) 270
TspRI CASTG 2 cut(s) 309, 576
Tth111I GACNNNGTC 1 cut(s) 64
XmaI CCCGGG 1 cut(s) 270
XmiI GTMKAC 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.