pycom10g25440

Galactose binding lectin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0016929
Physical Location & Seq
Reverse (-)
27083652 .. 27088651
5000 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2553 bp
ATGATGGGAAGCAAGAATTGGGTAGCATTAGTTCTGTTTATCCTTTTAGTTTTTGAAGGTTATTTCACTGAAACTGTGGTTTCGGCCACCGTGACATATGACCACAGAGCACTGGTTATTGATGGGAAGCGGCGGATTTTGCAGTCGGGCTCTTTTCATTACCCTCGAACCACTCCGGAGGTGTGGCCGGAAATTATTGAGAAAGCGAAGGAAGGGGGAATAGACGTGATTGAGACTTACGTTTTTTGGAACTACCATGAGCCAGTAAAAGGCCAGTACTACTTTGAAGGCAGATTTGATTTGGTGAAATTTGTGAAGACAGTCCAAGAAGCAGGTCTTCTAGTTCATTTGAGGATTGGCCCGTATGCTTGTGCTGAATGGAACTACGGGGGATTTCCAATTTGGTTACACTTTATTCCCGGAATTCAGTTTCGCACAACAAATGCACCTTTTAAGAAGGAAATGAAGATATTTCTTGCAAAAATTGTTGAAATGATGAAAATGGAGAATCTCTTCGCATCACAAGGAGGCCCAATCATTCTTGCTCAGGTTGAAAATGAATTTGGAAATGTTGAAGGGTCGTATGGAGTTGGTGGAGAATTATATGTCAAATGGGCTGCAGAAACTGCTGTTAGTCTCAATACATCTGTACCTTGGGTGATGTGTGTGCAAGATGACGCCCCGGATCCAGTTATAAACACATGCAATGGATTTTATTGCGATGAGTTTACCCCGAATTCCCCATCCAAACCGAAGATGTGGACAGAGAATTACAGTGGATGGTTCCTTGGATTTGGCAATCCAATCCCCTTTCGACCTGTTGAGGACCTTGCTTTTGCTGTTGCACGATTTTTCGAAACAGGTGGCACTTTTCAAAACTATTACATGTATTTTGGCGGTACCAACTTTGGGCGAACTGCTGGAGGCCCTTTGGTTGCAACGAGCTATGATTATGATGCTCCTATAGATGAATATGGTTTTCGTAGACAGCCAAAATGGGGACACCTGCGAGACTTGCACCTGGCCATAAAACAGTGTGAAGAATATATGATCAGCTCTGATCCGACACAAATGAAACTTGGTACTAATTTAGAGGCACATATCTACCATAAAACATCCAATGAGTGTGCGGCATTTCTTGCTAATTCTGACAGTAGTTCAGATGCAAATGTCACTTTTAATGGAAATATCTATTTCCTTCCAGCTTGGTCTGTGAGCATCCTTCCGGATTGTAAGAATGTTATATTTAACACCGCTAAGGTTGTCTCACAAAGGAATATTGGACTTTCTCCTTTTGCCCTCACACCCGGTGTACATGATTTTATACTGGAACCAGCTTCATGGAGTTGGTATAAAGAGAGAGTTGGTACTTGGAGTAACAATTCGTATACTGACTCGGGCTTACTGGAGCAGATAAGTACTACGAAAGATGTTAGTGATTATTTATGGTATACAATAAGCATTAATGTGGACGCAAACATAATACCCCGTCAAGAAAATGAACTTTTTTTACACATCGAGAGTCTGGGACATGCAGCCCTAGTTTTCTTAAATAAAAGATTTGTGGGATTTGGATATGGTAACCATGAAGATGCAAGTTTCATTGTTGACGAGAAGATTACTCTAAACCATGGAAACAATACCATAGATTTGTTGAGTATGATGATTGGTGTACAGAATTATGGGCCGTGGTTCGATGTTGCAGGAGCAGGAATCTTTTCTGTTGTTCTTAGAGACTTGAAGAACAGAACAAATGATCTTTCTTTTGAAGAATGGACCTACCAGGTGGGACTTGAGGGGGAATACCTTGGATTGGATAACATAAGCCTTGCGAACAGTTCACTGTGGACTACAGGGTCTGCTCCACCACTTAATCAGAGTTTGATATGGTACAAGGTTTCATTCCTTGCTCCTGAAGGCAAGGGTCCTTTGGCGTTAAATCTTGCTAGCATGGGAAAGGGACAAGCGTGGGTTAACGGGCAGAGTGTAGGGAGATATTGGCAAGCCTACCTCTCGCCCTCAACTGGTTGCATAAACGGAAGTGATTGTGACTACAGAGGAGCCTATAACGCATCTAAATGTCTAAGGAACTGTGGTCAACCGGCTCAAACTCTGTATCATATTCCCCGTACATGGGTACATACCGGAGATAACCTTCTAGTTCTCCACGAAGAGCTTGGTGGTGACCCTTCAAAGATTTCTTTGAGGACAAAAACCGGCCAAGAAATATGCGCACATGTATCTGAGACTGATCCTCCGCCTGCCAGTTCTTGGAAACCAGACTCGGAATTCATGTCTCAAAACCCTGAAGTTCAACTGACCTGCGAACAAGGATGGCGTATCACTTCAATCAATTTTGCTAGCTTTGGAACTCCTATTGGAAATTGTGGCACATTCGCTTCGGGAGCTTGCAGTGCTGACATCGTATCAATCGTTCAGCAGGCTTGCCTTGGCCAAGAAGGATGTTCAATCCCTATCTCCGCGGCAACCTTGGGCGATCCTTGTCCCGGAGTGCTTAAGAGTTTAGCTGTCGAAGCTATTTGCAGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

851

Amino Acids

94.35

Weight (kDa)

5.08

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 38 - 341 1.5e-114 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 58 - 190 5.5e-06 Beta-galactosidase
GHD PF17834 365 - 421 5.1e-27 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 628 - 718 2.5e-21 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 773 - 848 2.2e-16 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016929)

Species Orthologous Gene IDs
malus_domestica MD10G1301600.v1.1
prunus_persica Prupe.4G038600_v2.0.a1
pyrus_communis pycom10g25440
rosa_chinensis RchiOBHm_Chr5g0006691
rosa_laevigata RLG00000031417 RLG00000031511
rosa_multiflora Rmu_sc0017347.1_g000002 Rmu_sc0018387.1_g000009
rosa_roxburghii Rroxscaffold_1G00069330
rosa_rugosa Rorug04G0423700
rosa_samantha Rh5AG051500 Rh5CG059000
rosa_wichuraiana Rw5G005080 Rw5G005300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
AarI CACCTGC 1 cut(s) 1014
AasI GACNNNNNNGTC 1 cut(s) 1855
Acc16I TGCGCA 1 cut(s) 2233
Acc36I ACCTGC 3 cut(s) 323, 1014, 2330
Acc65I GGTACC 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 899
AccB7I CCANNNNNTGG 1 cut(s) 2271
AccI GTMKAC 3 cut(s) 985, 1388, 1451
AccII CGCG 1 cut(s) 2483
AccIII TCCGGA 2 cut(s) 175, 1225
AciI CCGC 8 cut(s) 130, 133, 897, 1130, 1254, 2258, 2481, 2483
AclWI GGATC 5 cut(s) 680, 693, 1055, 2246, 2492
AcoI YGGCCR 5 cut(s) 84, 185, 1023, 2218, 2452
AcsI RAATTY 5 cut(s) 308, 423, 560, 736, 2288
AcuI CTGAAG 2 cut(s) 1935, 2328
AcyI GRCGYC 1 cut(s) 678
AdeI CACNNNGTG 1 cut(s) 1310
AfiI CCNNNNNNNGG 9 cut(s) 269, 909, 998, 1813, 2024, 2133, 2134, 2271, 2507
AflII CTTAAG 1 cut(s) 2516
AflIII ACRYGT 2 cut(s) 885, 2236
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 2 cut(s) 1020, 1782
AjuI GAANNNNNNNTTGG 3 cut(s) 33, 546, 578
AluBI AGCT 9 cut(s) 945, 1056, 1205, 1337, 2176, 2364, 2408, 2528, 2537
AluI AGCT 9 cut(s) 945, 1056, 1205, 1337, 2176, 2364, 2408, 2528, 2537
Alw21I GWGCWC 1 cut(s) 112
Alw26I GTCTC 7 cut(s) 227, 641, 1005, 1270, 1728, 2240, 2301
AlwI GGATC 5 cut(s) 680, 693, 1055, 2246, 2492
AlwNI CAGNNNCTG 2 cut(s) 626, 1859
Ama87I CYCGRG 1 cut(s) 1396
Aor13HI TCCGGA 2 cut(s) 175, 1225
ApeKI GCWGC 2 cut(s) 617, 1535
ApoI RAATTY 5 cut(s) 308, 423, 560, 736, 2288
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
AseI ATTAAT 1 cut(s) 1464
Asp700I GAANNNNTTC 2 cut(s) 512, 1717
Asp718I GGTACC 1 cut(s) 899
AspLEI GCGC 1 cut(s) 2234
AspS9I GGNCC 7 cut(s) 359, 530, 826, 926, 1685, 1776, 1925
AsuC2I CCSGG 4 cut(s) 420, 683, 1308, 2508
AsuHPI GGTGA 3 cut(s) 316, 670, 2195
AsuII TTCGAA 1 cut(s) 855
AsuNHI GCTAGC 2 cut(s) 1946, 2360
AvaI CYCGRG 1 cut(s) 1396
AvaII GGWCC 3 cut(s) 826, 1776, 1925
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
BalI TGGCCA 2 cut(s) 1025, 2454
BamHI GGATCC 1 cut(s) 685
BanI GGYRCC 1 cut(s) 899
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 2 cut(s) 323, 329
Bbv12I GWGCWC 1 cut(s) 112
BbvI GCAGC 2 cut(s) 604, 1547
BccI CCATC 4 cut(s) 116, 751, 774, 2328
BceAI ACGGC 1 cut(s) 1672
BciT130I CCWGG 2 cut(s) 1022, 1784
BclI TGATCA 1 cut(s) 1050
BcnI CCSGG 4 cut(s) 420, 683, 1308, 2508
BcoDI GTCTC 7 cut(s) 227, 641, 1005, 1270, 1728, 2240, 2301
BfaI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
BfmI CTRYAG 4 cut(s) 618, 963, 1851, 2053
BfrI CTTAAG 1 cut(s) 2516
BfuAI ACCTGC 3 cut(s) 323, 1014, 2330
BisI GCNGC 5 cut(s) 131, 618, 1131, 1536, 2484
BlsI GCNGC 5 cut(s) 132, 619, 1132, 1537, 2485
BmcAI AGTACT 2 cut(s) 278, 1420
Bme1390I CCNGG 6 cut(s) 420, 683, 1022, 1308, 1784, 2508
Bme18I GGWCC 3 cut(s) 826, 1776, 1925
BmeT110I CYCGRG 1 cut(s) 1396
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 7 cut(s) 359, 530, 826, 926, 1685, 1776, 1925
BmiI GGNNCC 6 cut(s) 687, 785, 901, 1332, 1926, 2062
BmrFI CCNGG 6 cut(s) 420, 683, 1022, 1308, 1784, 2508
BmsI GCATC 6 cut(s) 527, 946, 1153, 1227, 1582, 2081
BmtI GCTAGC 2 cut(s) 1950, 2364
BpiI GAAGAC 2 cut(s) 323, 329
BpmI CTGGAG 2 cut(s) 942, 1427
Bpu10I CCTNAGC 2 cut(s) 546, 1257
Bpu14I TTCGAA 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 1814
BpuMI CCSGG 4 cut(s) 420, 683, 1308, 2508
BsaHI GRCGYC 1 cut(s) 678
BsaJI CCNNGG 9 cut(s) 653, 681, 787, 1630, 1688, 1807, 2449, 2481, 2490
BsaWI WCCGGW 3 cut(s) 175, 1225, 2144
BsaXI ACNNNNNCTCC 2 cut(s) 2239, 2269
Bsc4I CCNNNNNNNGG 9 cut(s) 269, 909, 998, 1813, 2024, 2133, 2134, 2271, 2507
Bse118I RCCGGY 2 cut(s) 2101, 2216
Bse1I ACTGG 8 cut(s) 117, 263, 274, 689, 1332, 1410, 2029, 2265
Bse3DI GCAATG 1 cut(s) 712
BseAI TCCGGA 2 cut(s) 175, 1225
BseBI CCWGG 2 cut(s) 1022, 1784
BseDI CCNNGG 9 cut(s) 653, 681, 787, 1630, 1688, 1807, 2449, 2481, 2490
BseGI GGATG 6 cut(s) 743, 785, 1115, 1218, 2339, 2468
BseLI CCNNNNNNNGG 9 cut(s) 269, 909, 998, 1813, 2024, 2133, 2134, 2271, 2507
BseMI GCAATG 1 cut(s) 712
BseMII CTCAG 2 cut(s) 560, 2235
BseNI ACTGG 8 cut(s) 117, 263, 274, 689, 1332, 1410, 2029, 2265
BseRI GAGGAG 1 cut(s) 2073
BseXI GCAGC 2 cut(s) 604, 1547
Bsh1236I CGCG 1 cut(s) 2483
BshNI GGYRCC 1 cut(s) 899
BsiHKAI GWGCWC 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 1396
BslFI GGGAC 5 cut(s) 1014, 1542, 1803, 1974, 2490
BslI CCNNNNNNNGG 9 cut(s) 269, 909, 998, 1813, 2024, 2133, 2134, 2271, 2507
BsmAI GTCTC 7 cut(s) 227, 641, 1005, 1270, 1728, 2240, 2301
BsmFI GGGAC 5 cut(s) 1014, 1542, 1803, 1974, 2490
BsoBI CYCGRG 1 cut(s) 1396
Bsp119I TTCGAA 1 cut(s) 855
Bsp1286I GDGCHC 2 cut(s) 112, 152
Bsp13I TCCGGA 2 cut(s) 175, 1225
Bsp1407I TGTACA 2 cut(s) 1312, 1672
Bsp143I GATC 6 cut(s) 685, 1050, 1060, 1756, 2251, 2497
Bsp19I CCATGG 1 cut(s) 1630
BspACI CCGC 8 cut(s) 130, 133, 897, 1130, 1254, 2258, 2481, 2483
BspCNI CTCAG 2 cut(s) 559, 2236
BspEI TCCGGA 2 cut(s) 175, 1225
BspFNI CGCG 1 cut(s) 2483
BspLI GGNNCC 6 cut(s) 687, 785, 901, 1332, 1926, 2062
BspMAI CTGCAG 1 cut(s) 622
BspMI ACCTGC 3 cut(s) 323, 1014, 2330
BspOI GCTAGC 2 cut(s) 1950, 2364
BspPI GGATC 5 cut(s) 680, 693, 1055, 2246, 2492
BspQI GCTCTTC 1 cut(s) 2166
BspT104I TTCGAA 1 cut(s) 855
BspT107I GGYRCC 1 cut(s) 899
BspTI CTTAAG 1 cut(s) 2516
BsrDI GCAATG 1 cut(s) 712
BsrFI RCCGGY 2 cut(s) 2101, 2216
BsrGI TGTACA 2 cut(s) 1312, 1672
BsrI ACTGG 8 cut(s) 117, 263, 274, 689, 1332, 1410, 2029, 2265
BssAI RCCGGY 2 cut(s) 2101, 2216
BssECI CCNNGG 9 cut(s) 653, 681, 787, 1630, 1688, 1807, 2449, 2481, 2490
BssMI GATC 6 cut(s) 685, 1050, 1060, 1756, 2251, 2497
BssNAI GTATAC 2 cut(s) 1389, 1452
BssNI GRCGYC 1 cut(s) 678
BssT1I CCWWGG 6 cut(s) 653, 787, 1630, 1807, 2449, 2490
Bst1107I GTATAC 2 cut(s) 1389, 1452
Bst2UI CCWGG 2 cut(s) 1022, 1784
Bst4CI ACNGT 9 cut(s) 76, 91, 322, 776, 1035, 1154, 1838, 1845, 2093
Bst6I CTCTTC 2 cut(s) 518, 2166
BstACI GRCGYC 1 cut(s) 678
BstAFI CTTAAG 1 cut(s) 2516
BstAPI GCANNNNNTGC 2 cut(s) 626, 1139
BstAUI TGTACA 2 cut(s) 1312, 1672
BstBI TTCGAA 1 cut(s) 855
BstC8I GCNNGC 7 cut(s) 1948, 2004, 2262, 2362, 2410, 2442, 2446
BstDEI CTNAG 5 cut(s) 546, 1257, 1730, 2084, 2244
BstDSI CCRYGG 3 cut(s) 1630, 1688, 2481
BstEII GGTNACC 2 cut(s) 1580, 2183
BstF5I GGATG 6 cut(s) 743, 785, 1115, 1218, 2339, 2468
BstFNI CGCG 1 cut(s) 2483
BstHHI GCGC 1 cut(s) 2234
BstKTI GATC 6 cut(s) 688, 1053, 1063, 1759, 2254, 2500
BstMAI GTCTC 7 cut(s) 227, 641, 1005, 1270, 1728, 2240, 2301
BstMBI GATC 6 cut(s) 685, 1050, 1060, 1756, 2251, 2497
BstMWI GCNNNNNNNGC 8 cut(s) 139, 626, 1015, 1139, 2069, 2405, 2414, 2534
BstNI CCWGG 2 cut(s) 1022, 1784
BstNSI RCATGY 4 cut(s) 705, 889, 1535, 2240
BstPI GGTNACC 2 cut(s) 1580, 2183
BstSCI CCNGG 6 cut(s) 418, 681, 1020, 1306, 1782, 2506
BstSFI CTRYAG 4 cut(s) 618, 963, 1851, 2053
BstUI CGCG 1 cut(s) 2483
BstV1I GCAGC 2 cut(s) 604, 1547
BstV2I GAAGAC 2 cut(s) 323, 329
BstX2I RGATCY 1 cut(s) 685
BstXI CCANNNNNNTGG 1 cut(s) 1341
BstYI RGATCY 1 cut(s) 685
BstZ17I GTATAC 2 cut(s) 1389, 1452
BtgI CCRYGG 3 cut(s) 1630, 1688, 2481
BtgZI GCGATG 1 cut(s) 735
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 6 cut(s) 743, 785, 1115, 1218, 2339, 2468
BtsI GCAGTG 2 cut(s) 2419, 2551
BtsIMutI CAGTG 7 cut(s) 66, 110, 781, 1040, 1841, 2419, 2551
BveI ACCTGC 3 cut(s) 323, 1014, 2330
Cac8I GCNNGC 7 cut(s) 1948, 2004, 2262, 2362, 2410, 2442, 2446
CaiI CAGNNNCTG 2 cut(s) 626, 1859
CfoI GCGC 1 cut(s) 2234
Cfr10I RCCGGY 2 cut(s) 2101, 2216
Cfr13I GGNCC 7 cut(s) 359, 530, 826, 926, 1685, 1776, 1925
Cfr42I CCGCGG 1 cut(s) 2484
CseI GACGC 2 cut(s) 686, 1481
CsiI ACCWGGT 1 cut(s) 1782
DdeI CTNAG 5 cut(s) 546, 1257, 1730, 2084, 2244
DpnI GATC 6 cut(s) 687, 1052, 1062, 1758, 2253, 2499
DpnII GATC 6 cut(s) 685, 1050, 1060, 1756, 2251, 2497
DraIII CACNNNGTG 1 cut(s) 1310
DrdI GACNNNNNNGTC 1 cut(s) 1855
DseDI GACNNNNNNGTC 1 cut(s) 1855
EaeI YGGCCR 5 cut(s) 84, 185, 1023, 2218, 2452
Eam1104I CTCTTC 2 cut(s) 518, 2166
EarI CTCTTC 2 cut(s) 518, 2166
EciI GGCGGA 2 cut(s) 148, 2247
Eco130I CCWWGG 6 cut(s) 653, 787, 1630, 1807, 2449, 2490
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 3 cut(s) 826, 1776, 1925
Eco57I CTGAAG 2 cut(s) 1935, 2328
Eco88I CYCGRG 1 cut(s) 1396
Eco91I GGTNACC 2 cut(s) 1580, 2183
EcoO109I RGGNCCY 3 cut(s) 826, 926, 1925
EcoO65I GGTNACC 2 cut(s) 1580, 2183
EcoRI GAATTC 3 cut(s) 423, 736, 2288
EcoRII CCWGG 2 cut(s) 1020, 1782
EcoT14I CCWWGG 6 cut(s) 653, 787, 1630, 1807, 2449, 2490
EcoT38I GRGCYC 1 cut(s) 152
ErhI CCWWGG 6 cut(s) 653, 787, 1630, 1807, 2449, 2490
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 5 cut(s) 1014, 1542, 1803, 1974, 2490
FauNDI CATATG 1 cut(s) 97
FbaI TGATCA 1 cut(s) 1050
FblI GTMKAC 3 cut(s) 985, 1388, 1451
Fnu4HI GCNGC 5 cut(s) 131, 618, 1131, 1536, 2484
FokI GGATG 6 cut(s) 730, 792, 1102, 1205, 2346, 2475
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 5 cut(s) 131, 618, 1131, 1536, 2484
FspAI RTGCGCAY 1 cut(s) 2233
FspBI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
FspI TGCGCA 1 cut(s) 2233
GlaI GCGC 1 cut(s) 2233
GluI GCNGC 5 cut(s) 131, 618, 1131, 1536, 2484
GsuI CTGGAG 2 cut(s) 942, 1427
HgaI GACGC 2 cut(s) 686, 1481
HhaI GCGC 1 cut(s) 2234
Hin1I GRCGYC 1 cut(s) 678
Hin6I GCGC 1 cut(s) 2232
HinP1I GCGC 1 cut(s) 2232
HincII GTYRAC 3 cut(s) 1609, 1975, 2099
HindII GTYRAC 3 cut(s) 1609, 1975, 2099
HinfI GANTC 5 cut(s) 508, 1394, 1522, 1713, 2282
HpaI GTTAAC 1 cut(s) 1975
HphI GGTGA 3 cut(s) 316, 670, 2195
Hpy188I TCNGA 7 cut(s) 1060, 1065, 1150, 1162, 1878, 2245, 2287
Hpy188III TCNNGA 6 cut(s) 176, 1226, 1493, 1519, 1913, 2403
HpyCH4III ACNGT 9 cut(s) 76, 91, 322, 776, 1035, 1154, 1838, 1845, 2093
HpyCH4IV ACGT 2 cut(s) 225, 240
HpyF10VI GCNNNNNNNGC 8 cut(s) 139, 626, 1015, 1139, 2069, 2405, 2414, 2534
HpyF3I CTNAG 5 cut(s) 546, 1257, 1730, 2084, 2244
HpySE526I ACGT 2 cut(s) 225, 240
Hsp92I GRCGYC 1 cut(s) 678
HspAI GCGC 1 cut(s) 2232
Kpn2I TCCGGA 2 cut(s) 175, 1225
KpnI GGTACC 1 cut(s) 903
Ksp22I TGATCA 1 cut(s) 1050
KspAI GTTAAC 1 cut(s) 1975
KspI CCGCGG 1 cut(s) 2484
Kzo9I GATC 6 cut(s) 685, 1050, 1060, 1756, 2251, 2497
LguI GCTCTTC 1 cut(s) 2166
LmnI GCTCC 7 cut(s) 964, 1408, 1706, 1867, 1915, 2060, 2405
Lsp1109I GCAGC 2 cut(s) 604, 1547
LweI GCATC 6 cut(s) 527, 946, 1153, 1227, 1582, 2081
MabI ACCWGGT 1 cut(s) 1782
MaeI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
MaeII ACGT 2 cut(s) 225, 240
MaeIII GTNAC 7 cut(s) 91, 405, 1171, 1376, 1580, 2048, 2183
MalI GATC 6 cut(s) 687, 1052, 1062, 1758, 2253, 2499
MboI GATC 6 cut(s) 685, 1050, 1060, 1756, 2251, 2497
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 2 cut(s) 112, 152
MlsI TGGCCA 2 cut(s) 1025, 2454
MluNI TGGCCA 2 cut(s) 1025, 2454
MlyI GAGTC 3 cut(s) 1388, 1531, 2276
MmeI TCCRAC 1 cut(s) 1088
Mox20I TGGCCA 2 cut(s) 1025, 2454
MroI TCCGGA 2 cut(s) 175, 1225
MroXI GAANNNNTTC 2 cut(s) 512, 1717
MscI TGGCCA 2 cut(s) 1025, 2454
MseI TTAA 9 cut(s) 453, 1179, 1248, 1464, 1550, 1872, 1937, 1974, 2517
MslI CAYNNNNRTG 3 cut(s) 1466, 1590, 2077
Msp20I TGGCCA 2 cut(s) 1025, 2454
MspA1I CMGCKG 1 cut(s) 2483
MspCI CTTAAG 1 cut(s) 2516
MspR9I CCNGG 6 cut(s) 420, 683, 1022, 1308, 1784, 2508
MvaI CCWGG 2 cut(s) 1022, 1784
MvnI CGCG 1 cut(s) 2483
MwoI GCNNNNNNNGC 8 cut(s) 139, 626, 1015, 1139, 2069, 2405, 2414, 2534
NciI CCSGG 4 cut(s) 420, 683, 1308, 2508
NcoI CCATGG 1 cut(s) 1630
NdeI CATATG 1 cut(s) 97
NdeII GATC 6 cut(s) 685, 1050, 1060, 1756, 2251, 2497
NheI GCTAGC 2 cut(s) 1946, 2360
NlaIV GGNNCC 6 cut(s) 687, 785, 901, 1332, 1926, 2062
NmuCI GTSAC 4 cut(s) 91, 1171, 2048, 2183
NsbI TGCGCA 1 cut(s) 2233
NspI RCATGY 4 cut(s) 705, 889, 1535, 2240
NspV TTCGAA 1 cut(s) 855
PaqCI CACCTGC 1 cut(s) 1014
PciI ACATGT 2 cut(s) 885, 2236
PciSI GCTCTTC 1 cut(s) 2166
PcsI WCGNNNNNNNCGW 1 cut(s) 2430
PdmI GAANNNNTTC 2 cut(s) 512, 1717
PfeI GAWTC 2 cut(s) 508, 1713
PflMI CCANNNNNTGG 1 cut(s) 2271
PfoI TCCNGGA 2 cut(s) 418, 2506
PkrI GCNGC 5 cut(s) 132, 619, 1132, 1537, 2485
PleI GAGTC 3 cut(s) 1388, 1530, 2276
PpsI GAGTC 3 cut(s) 1388, 1530, 2276
PpuMI RGGWCCY 2 cut(s) 826, 1925
PscI ACATGT 2 cut(s) 885, 2236
PshBI ATTAAT 1 cut(s) 1464
PsiI TTATAA 1 cut(s) 695
Psp5II RGGWCCY 2 cut(s) 826, 1925
Psp6I CCWGG 2 cut(s) 1020, 1782
PspEI GGTNACC 2 cut(s) 1580, 2183
PspGI CCWGG 2 cut(s) 1020, 1782
PspN4I GGNNCC 6 cut(s) 687, 785, 901, 1332, 1926, 2062
PspPI GGNCC 7 cut(s) 359, 530, 826, 926, 1685, 1776, 1925
PspPPI RGGWCCY 2 cut(s) 826, 1925
PsrI GAACNNNNNNTAC 6 cut(s) 15, 47, 1494, 1526, 2418, 2450
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 2 cut(s) 626, 1859
PsuI RGATCY 1 cut(s) 685
RseI CAYNNNNRTG 3 cut(s) 1466, 1590, 2077
SacII CCGCGG 1 cut(s) 2484
SapI GCTCTTC 1 cut(s) 2166
SaqAI TTAA 9 cut(s) 453, 1179, 1248, 1464, 1550, 1872, 1937, 1974, 2517
SatI GCNGC 5 cut(s) 131, 618, 1131, 1536, 2484
Sau3AI GATC 6 cut(s) 685, 1050, 1060, 1756, 2251, 2497
Sau96I GGNCC 7 cut(s) 359, 530, 826, 926, 1685, 1776, 1925
ScaI AGTACT 2 cut(s) 278, 1420
SchI GAGTC 3 cut(s) 1388, 1531, 2276
ScrFI CCNGG 6 cut(s) 420, 683, 1022, 1308, 1784, 2508
SduI GDGCHC 2 cut(s) 112, 152
SexAI ACCWGGT 1 cut(s) 1782
SfaNI GCATC 6 cut(s) 527, 946, 1153, 1227, 1582, 2081
SfcI CTRYAG 4 cut(s) 618, 963, 1851, 2053
Sfr303I CCGCGG 1 cut(s) 2484
SfuI TTCGAA 1 cut(s) 855
SgrBI CCGCGG 1 cut(s) 2484
SinI GGWCC 3 cut(s) 826, 1776, 1925
SmiMI CAYNNNNRTG 3 cut(s) 1466, 1590, 2077
SmlI CTYRAG 2 cut(s) 1793, 2516
SmoI CTYRAG 2 cut(s) 1793, 2516
SsiI CCGC 8 cut(s) 130, 133, 897, 1130, 1254, 2258, 2481, 2483
SspI AATATT 1 cut(s) 1279
SspMI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
StyD4I CCNGG 6 cut(s) 418, 681, 1020, 1306, 1782, 2506
StyI CCWWGG 6 cut(s) 653, 787, 1630, 1807, 2449, 2490
TaaI ACNGT 9 cut(s) 76, 91, 322, 776, 1035, 1154, 1838, 1845, 2093
TaiI ACGT 2 cut(s) 228, 243
TaqI TCGA 6 cut(s) 166, 814, 855, 1518, 1695, 2532
TatI WGTACW 4 cut(s) 276, 1312, 1418, 1672
TauI GCSGC 3 cut(s) 133, 1133, 2486
TfiI GAWTC 2 cut(s) 508, 1713
Tru1I TTAA 9 cut(s) 453, 1179, 1248, 1464, 1550, 1872, 1937, 1974, 2517
Tru9I TTAA 9 cut(s) 453, 1179, 1248, 1464, 1550, 1872, 1937, 1974, 2517
TscAI CASTG 7 cut(s) 73, 117, 781, 1040, 1848, 2419, 2551
TseFI GTSAC 4 cut(s) 91, 1171, 2048, 2183
TseI GCWGC 2 cut(s) 617, 1535
Tsp45I GTSAC 4 cut(s) 91, 1171, 2048, 2183
TspGWI ACGGA 1 cut(s) 2052
TspRI CASTG 7 cut(s) 73, 117, 781, 1040, 1848, 2419, 2551
Van91I CCANNNNNTGG 1 cut(s) 2271
Vha464I CTTAAG 1 cut(s) 2516
VpaK11BI GGWCC 3 cut(s) 826, 1776, 1925
VspI ATTAAT 1 cut(s) 1464
XapI RAATTY 5 cut(s) 308, 423, 560, 736, 2288
XceI RCATGY 4 cut(s) 705, 889, 1535, 2240
XcmI CCANNNNNNNNNTGG 1 cut(s) 2174
XmiI GTMKAC 3 cut(s) 985, 1388, 1451
XmnI GAANNNNTTC 2 cut(s) 512, 1717
XspI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
ZrmI AGTACT 2 cut(s) 278, 1420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.