pycom10g27220

ATP synthase gamma chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015012
Physical Location & Seq
Forward (+)
28253899 .. 28255023
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1125 bp
ATGTCTTGCTCGAATTTGACAATGTGGGTGTCCTCAAAACCCTCCCTTTCCGACGCCTCCGCCCTCTCCTGCCGCTCCTTCATCGCCCCTCTTCAGCTTCCCTCCCAAAACTCCCTTCCCGCCTCGCGATCGTCATCTGTGACACCGGTTCAATGCGGTCTACGCGAGCTTCGAGAGCGTATTTCATCAGTGAAGAACACGCAGAAGATCACTGAGGCTATGAAGCTTGTGGCAGCCGCCAAAGTCCGCCGAGCTCAAGAGGCTGTCGTTAATGGCAGACCCTTCTCGGAAACCCTAGTTGAAGTTCTTTACAACATCAATGAGCAGCTCCAAGTGGAAGACATTGATGCTCCTCTGACCAAAGTTAGGCCAGTCAAGAAAGTTGCTCTCGTTGTCATAACCGGGGATCGCGGTCTTTGCGGAGGGTTCAACAATATGATCATCAAAAAAGCAGAGAGAAGAATAGCGGAATTGAAGGCTCTTGGTCTCGAATTCACCATCATCAGCGTCGGTAAAAAGGGTAACTCGTATTTCCTCCGCCGGCCTTACATTCCGGTTGATAAGTTTCTCGAGGGAACCAACCTCCCAACCGCCAAAGAAGCGCAGGCCATCGCCGATGATGTTTTCTCCCTTTTCGTGAGCGAAGAGGTCGACAAAGTCGAGCTTTTGTACACGAAATTCGTGTCGCTGGTTAAATCCGACCCTGTGATTCACACCCTGCTTCCACTCTCGCCAAAAGGAGAGATTTGCGACATCAATGGCGTTTGTGTGGACGCCGTGGATGATGAGTTCTTCAGGCTGACAACAAAGGAAGGGAAATTGACAGTGGAGAGAGATGTCATCAAGACGCAGACCGTGGATTTCACTCCGGTTTTACAGTTTGAGCAGGATCCGGTTCAGATTCTCGACGCTTTGCTGCCCTTGTACCTCAACAGTCAGATTTTGAGGGCATTGCAGGAGTCTCTAGCCAGTGAGCTTGCTGCTAGGATGACCGCTATGAGCAATGCAACCGATAACGCTGTTGAGTTGAAGAAAACCTTGTCGCAGGCCTACAATCGGCAGCGCCAGGCAAAAATCACCGGAGAGATTTTGGAGATTGTTTCGGGTGCCAACGCTTTGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009544 GO:0009579 GO:0009767 GO:0009772 GO:0009941 GO:0009987 GO:0015672 GO:0015979 GO:0015985 GO:0015986 GO:0016020 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019684 GO:0019693 GO:0022900 GO:0030234 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0034220 GO:0034357 GO:0034641 GO:0034654 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046034 GO:0046390 GO:0046483 GO:0050790 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0055086 GO:0055114 GO:0065007 GO:0065009 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098655 GO:0098660 GO:0098662 GO:0098772 GO:0098796 GO:0098807 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko00195 ko01100 ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map00190 map00195 map01100 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
ATP-synt

Protein Analysis

375

Amino Acids

41.14

Weight (kDa)

7.51

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt PF00231 54 - 373 1e-93 ATP synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015012)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04640
fragaria_vesca FvH4_3g02060
malus_domestica MD05G1347300.v1.1 MD10G1321500.v1.1
prunus_persica Prupe.4G020800_v2.0.a1
pyrus_communis pycom05g31500 pycom10g27220
rosa_chinensis RchiOBHm_Chr5g0002961
rosa_laevigata RLG00000031074
rosa_multiflora Rmu_sc0013603.1_g000002
rosa_rugosa Rorug04G0400900
rosa_samantha Rh5AG023900 Rh5BG026600 Rh5CG026700 Rh5DG025200
rosa_wichuraiana Rw5G002260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1106
AccBSI CCGCTC 1 cut(s) 75
AccI GTMKAC 2 cut(s) 160, 651
AccII CGCG 3 cut(s) 127, 165, 411
AclWI GGATC 3 cut(s) 414, 884, 897
AcsI RAATTY 3 cut(s) 13, 491, 677
AcuI CTGAAG 2 cut(s) 77, 778
AcyI GRCGYC 2 cut(s) 54, 774
AfaI GTAC 2 cut(s) 671, 926
AfiI CCNNNNNNNGG 2 cut(s) 366, 1056
AgeI ACCGGT 1 cut(s) 145
AgsI TTSAA 5 cut(s) 152, 302, 430, 475, 1030
AjnI CCWGG 1 cut(s) 1065
AjuI GAANNNNNNNTTGG 2 cut(s) 99, 131
AloI GAACNNNNNNTCC 2 cut(s) 773, 805
AluBI AGCT 7 cut(s) 97, 169, 226, 254, 328, 664, 976
AluI AGCT 7 cut(s) 97, 169, 226, 254, 328, 664, 976
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 2 cut(s) 491, 966
AlwI GGATC 3 cut(s) 414, 884, 897
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 4 cut(s) 368, 542, 606, 1047
ApeKI GCWGC 5 cut(s) 233, 325, 916, 980, 1060
ApoI RAATTY 3 cut(s) 13, 491, 677
ArsI GACNNNNNNTTYG 2 cut(s) 668, 700
AsiGI ACCGGT 1 cut(s) 145
AspLEI GCGC 2 cut(s) 604, 1065
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 487, 1069
AvaI CYCGRG 1 cut(s) 569
BamHI GGATCC 1 cut(s) 889
BanI GGYRCC 1 cut(s) 1106
BanII GRGCYC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 5 cut(s) 245, 337, 903, 967, 1072
BccI CCATC 2 cut(s) 506, 617
BceAI ACGGC 1 cut(s) 761
BciT130I CCWGG 1 cut(s) 1067
BclI TGATCA 1 cut(s) 438
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 491, 966
BfaI CTAG 4 cut(s) 296, 965, 984, 1123
BfoI RGCGCY 1 cut(s) 1066
BisI GCNGC 7 cut(s) 73, 234, 237, 326, 917, 981, 1061
BlsI GCNGC 7 cut(s) 74, 235, 238, 327, 918, 982, 1062
Bme1390I CCNGG 2 cut(s) 403, 1067
BmeT110I CYCGRG 1 cut(s) 569
BmiI GGNNCC 3 cut(s) 577, 891, 1108
BmrFI CCNGG 2 cut(s) 403, 1067
BmsI GCATC 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 240
BpuMI CCSGG 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 133
BsaHI GRCGYC 2 cut(s) 54, 774
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 3 cut(s) 402, 777, 855
BsaWI WCCGGW 5 cut(s) 145, 553, 868, 892, 1079
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 1056
Bse118I RCCGGY 2 cut(s) 145, 540
Bse1I ACTGG 2 cut(s) 371, 969
Bse3DI GCAATG 2 cut(s) 950, 1009
Bse8I GATNNNNATC 1 cut(s) 133
BseBI CCWGG 1 cut(s) 1067
BseDI CCNNGG 3 cut(s) 402, 777, 855
BseGI GGATG 2 cut(s) 787, 993
BseJI GATNNNNATC 1 cut(s) 133
BseLI CCNNNNNNNGG 2 cut(s) 366, 1056
BseMI GCAATG 2 cut(s) 950, 1009
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 371, 969
BseRI GAGGAG 1 cut(s) 342
BseXI GCAGC 5 cut(s) 245, 337, 903, 967, 1072
Bsh1236I CGCG 3 cut(s) 127, 165, 411
Bsh1285I CGRYCG 1 cut(s) 131
BshFI GGCC 4 cut(s) 370, 544, 608, 1049
BshNI GGYRCC 1 cut(s) 1106
BshTI ACCGGT 1 cut(s) 145
BsiEI CGRYCG 1 cut(s) 131
BsiHKAI GWGCWC 1 cut(s) 256
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 7 cut(s) 146, 402, 541, 554, 869, 893, 1080
BslI CCNNNNNNNGG 2 cut(s) 366, 1056
BsmAI GTCTC 2 cut(s) 491, 966
BsnI GGCC 4 cut(s) 370, 544, 608, 1049
Bso31I GGTCTC 1 cut(s) 491
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 256
Bsp1407I TGTACA 1 cut(s) 669
Bsp143I GATC 5 cut(s) 128, 207, 406, 438, 889
Bsp68I TCGCGA 1 cut(s) 127
BspANI GGCC 4 cut(s) 370, 544, 608, 1049
BspCNI CTCAG 1 cut(s) 205
BspFNI CGCG 3 cut(s) 127, 165, 411
BspLI GGNNCC 3 cut(s) 577, 891, 1108
BspPI GGATC 3 cut(s) 414, 884, 897
BspT107I GGYRCC 1 cut(s) 1106
BspTNI GGTCTC 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 75
BsrDI GCAATG 2 cut(s) 950, 1009
BsrFI RCCGGY 2 cut(s) 145, 540
BsrGI TGTACA 1 cut(s) 669
BsrI ACTGG 2 cut(s) 371, 969
BssAI RCCGGY 2 cut(s) 145, 540
BssECI CCNNGG 3 cut(s) 402, 777, 855
BssMI GATC 5 cut(s) 128, 207, 406, 438, 889
BssNI GRCGYC 2 cut(s) 54, 774
Bst2UI CCWGG 1 cut(s) 1067
Bst4CI ACNGT 4 cut(s) 826, 856, 879, 935
Bst6I CTCTTC 2 cut(s) 96, 639
BstACI GRCGYC 2 cut(s) 54, 774
BstAUI TGTACA 1 cut(s) 669
BstC8I GCNNGC 5 cut(s) 167, 542, 606, 978, 1047
BstDEI CTNAG 1 cut(s) 213
BstDSI CCRYGG 2 cut(s) 777, 855
BstF5I GGATG 2 cut(s) 787, 993
BstFNI CGCG 3 cut(s) 127, 165, 411
BstH2I RGCGCY 1 cut(s) 1066
BstHHI GCGC 2 cut(s) 604, 1065
BstKTI GATC 5 cut(s) 131, 210, 409, 441, 892
BstMAI GTCTC 2 cut(s) 491, 966
BstMBI GATC 5 cut(s) 128, 207, 406, 438, 889
BstMCI CGRYCG 1 cut(s) 131
BstMWI GCNNNNNNNGC 5 cut(s) 162, 175, 260, 417, 599
BstNI CCWGG 1 cut(s) 1067
BstSCI CCNGG 2 cut(s) 401, 1065
BstUI CGCG 3 cut(s) 127, 165, 411
BstV1I GCAGC 5 cut(s) 245, 337, 903, 967, 1072
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 889
BstXI CCANNNNNNTGG 1 cut(s) 1117
BstYI RGATCY 1 cut(s) 889
BsuRI GGCC 4 cut(s) 370, 544, 608, 1049
BtgI CCRYGG 2 cut(s) 777, 855
BtgZI GCGATG 2 cut(s) 67, 595
BtsCI GGATG 2 cut(s) 787, 993
BtsIMutI CAGTG 4 cut(s) 195, 210, 831, 976
BtuMI TCGCGA 1 cut(s) 127
Cac8I GCNNGC 5 cut(s) 167, 542, 606, 978, 1047
CfoI GCGC 2 cut(s) 604, 1065
Cfr10I RCCGGY 2 cut(s) 145, 540
CseI GACGC 5 cut(s) 62, 496, 782, 856, 917
Csp6I GTAC 2 cut(s) 670, 925
CspAI ACCGGT 1 cut(s) 145
CviQI GTAC 2 cut(s) 670, 925
DdeI CTNAG 1 cut(s) 213
DpnI GATC 5 cut(s) 130, 209, 408, 440, 891
DpnII GATC 5 cut(s) 128, 207, 406, 438, 889
Eam1104I CTCTTC 2 cut(s) 96, 639
EarI CTCTTC 2 cut(s) 96, 639
EciI GGCGGA 3 cut(s) 49, 236, 527
Ecl136II GAGCTC 1 cut(s) 254
Eco147I AGGCCT 1 cut(s) 1049
Eco24I GRGCYC 1 cut(s) 256
Eco31I GGTCTC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 254
Eco57I CTGAAG 2 cut(s) 77, 778
Eco88I CYCGRG 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 254
EcoRI GAATTC 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 1065
EcoT38I GRGCYC 1 cut(s) 256
FaiI YATR 4 cut(s) 221, 398, 437, 998
FalI AAGNNNNNCTT 4 cut(s) 648, 680, 1022, 1054
FauI CCCGC 1 cut(s) 127
FbaI TGATCA 1 cut(s) 438
FblI GTMKAC 2 cut(s) 160, 651
Fnu4HI GCNGC 7 cut(s) 73, 234, 237, 326, 917, 981, 1061
FokI GGATG 2 cut(s) 794, 1000
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 7 cut(s) 73, 234, 237, 326, 917, 981, 1061
FspBI CTAG 4 cut(s) 296, 965, 984, 1123
GlaI GCGC 2 cut(s) 603, 1064
GluI GCNGC 7 cut(s) 73, 234, 237, 326, 917, 981, 1061
HaeII RGCGCY 1 cut(s) 1066
HaeIII GGCC 4 cut(s) 370, 544, 608, 1049
HapII CCGG 7 cut(s) 146, 402, 541, 554, 869, 893, 1080
HgaI GACGC 5 cut(s) 62, 496, 782, 856, 917
HhaI GCGC 2 cut(s) 604, 1065
Hin1I GRCGYC 2 cut(s) 54, 774
Hin6I GCGC 2 cut(s) 602, 1063
HinP1I GCGC 2 cut(s) 602, 1063
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 3 cut(s) 709, 901, 959
HpaII CCGG 7 cut(s) 146, 402, 541, 554, 869, 893, 1080
HphI GGTGA 2 cut(s) 487, 1069
Hpy166II GTNNAC 4 cut(s) 161, 652, 672, 772
Hpy188I TCNGA 6 cut(s) 52, 289, 357, 700, 900, 939
Hpy188III TCNNGA 9 cut(s) 126, 173, 257, 376, 488, 569, 637, 844, 905
Hpy8I GTNNAC 4 cut(s) 161, 652, 672, 772
Hpy99I CGWCG 3 cut(s) 56, 512, 911
HpyAV CCTTC 5 cut(s) 88, 125, 292, 469, 806
HpyCH4III ACNGT 4 cut(s) 826, 856, 879, 935
HpyCH4V TGCA 2 cut(s) 955, 1007
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 175, 260, 417, 599
HpyF3I CTNAG 1 cut(s) 213
Hsp92I GRCGYC 2 cut(s) 54, 774
HspAI GCGC 2 cut(s) 602, 1063
KroI GCCGGC 1 cut(s) 540
KroNI GCCGGC 1 cut(s) 542
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 5 cut(s) 128, 207, 406, 438, 889
LmnI GCTCC 3 cut(s) 80, 333, 355
Lsp1109I GCAGC 5 cut(s) 245, 337, 903, 967, 1072
LweI GCATC 1 cut(s) 337
MaeI CTAG 4 cut(s) 296, 965, 984, 1123
MaeIII GTNAC 2 cut(s) 139, 521
MalI GATC 5 cut(s) 130, 209, 408, 440, 891
MbiI CCGCTC 1 cut(s) 75
MboI GATC 5 cut(s) 128, 207, 406, 438, 889
MboII GAAGA 8 cut(s) 83, 205, 217, 350, 471, 656, 784, 1042
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 5 cut(s) 13, 470, 491, 677, 818
MlyI GAGTC 1 cut(s) 968
MmeI TCCRAC 2 cut(s) 75, 723
MroNI GCCGGC 1 cut(s) 540
MseI TTAA 2 cut(s) 270, 693
MspI CCGG 7 cut(s) 146, 402, 541, 554, 869, 893, 1080
MspR9I CCNGG 2 cut(s) 403, 1067
MvaI CCWGG 1 cut(s) 1067
MvnI CGCG 3 cut(s) 127, 165, 411
MwoI GCNNNNNNNGC 5 cut(s) 162, 175, 260, 417, 599
NaeI GCCGGC 1 cut(s) 542
NciI CCSGG 1 cut(s) 403
NdeII GATC 5 cut(s) 128, 207, 406, 438, 889
NgoMIV GCCGGC 1 cut(s) 540
NlaIV GGNNCC 3 cut(s) 577, 891, 1108
NmeAIII GCCGAG 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 139
NruI TCGCGA 1 cut(s) 127
PaeR7I CTCGAG 1 cut(s) 569
PceI AGGCCT 1 cut(s) 1049
PcsI WCGNNNNNNNCGW 2 cut(s) 169, 657
PdiI GCCGGC 1 cut(s) 542
PfeI GAWTC 2 cut(s) 709, 901
PflFI GACNNNGTC 1 cut(s) 656
PinAI ACCGGT 1 cut(s) 145
PkrI GCNGC 7 cut(s) 74, 235, 238, 327, 918, 982, 1062
Ple19I CGATCG 1 cut(s) 131
PleI GAGTC 1 cut(s) 967
PpsI GAGTC 1 cut(s) 967
Psp124BI GAGCTC 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 1065
PspGI CCWGG 1 cut(s) 1065
PspN4I GGNNCC 3 cut(s) 577, 891, 1108
PsuI RGATCY 1 cut(s) 889
PsyI GACNNNGTC 1 cut(s) 656
PvuI CGATCG 1 cut(s) 131
RruI TCGCGA 1 cut(s) 127
RsaI GTAC 2 cut(s) 671, 926
RsaNI GTAC 2 cut(s) 670, 925
SacI GAGCTC 1 cut(s) 256
SalI GTCGAC 1 cut(s) 650
SaqAI TTAA 2 cut(s) 270, 693
SatI GCNGC 7 cut(s) 73, 234, 237, 326, 917, 981, 1061
Sau3AI GATC 5 cut(s) 128, 207, 406, 438, 889
SchI GAGTC 1 cut(s) 968
ScrFI CCNGG 2 cut(s) 403, 1067
SduI GDGCHC 1 cut(s) 256
SfaNI GCATC 1 cut(s) 337
Sfr274I CTCGAG 1 cut(s) 569
SlaI CTCGAG 1 cut(s) 569
SmlI CTYRAG 2 cut(s) 255, 569
SmoI CTYRAG 2 cut(s) 255, 569
Sse9I AATT 5 cut(s) 13, 470, 491, 677, 818
SseBI AGGCCT 1 cut(s) 1049
SspMI CTAG 4 cut(s) 296, 965, 984, 1123
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 1 cut(s) 1049
StyD4I CCNGG 2 cut(s) 401, 1065
TaaI ACNGT 4 cut(s) 826, 856, 879, 935
TaqI TCGA 7 cut(s) 11, 172, 489, 570, 651, 660, 906
TasI AATT 5 cut(s) 13, 470, 491, 677, 818
TatI WGTACW 1 cut(s) 669
TauI GCSGC 2 cut(s) 75, 239
TfiI GAWTC 2 cut(s) 709, 901
Tru1I TTAA 2 cut(s) 270, 693
Tru9I TTAA 2 cut(s) 270, 693
TscAI CASTG 4 cut(s) 195, 217, 831, 976
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 5 cut(s) 233, 325, 916, 980, 1060
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 3 cut(s) 70, 174, 236
TspRI CASTG 4 cut(s) 195, 217, 831, 976
Tth111I GACNNNGTC 1 cut(s) 656
XapI RAATTY 3 cut(s) 13, 491, 677
XhoI CTCGAG 1 cut(s) 569
XmiI GTMKAC 2 cut(s) 160, 651
XspI CTAG 4 cut(s) 296, 965, 984, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.