pycom10g27510

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009756
Physical Location & Seq
Reverse (-)
28429566 .. 28430585
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 603 bp
ATGATTGTAAGCCTGGGAAGGGGTGGGAATGTTAGGAGCACTCCTACACATGTAGGGCAACGTCTAGGGAGCGACGGAGACGCCATATCTGTCTTTGCTTCATCGCATGTTTGGATTGACCATTGCTTCCTAGCTCGTAGCACAGATGGCCTCCTTGATGTCACCCATGCTTCCACTGCCGTCACCATCACAAACAACTATTTCTCTCAGCATGACAAAGTGATGCTGCTTGGTCACAATGACAATTTTAGTGAAGATAAAAATATGAGAGTCACAATAGCCTTCAACCGTTTTGGGGCTGGCCTTATTGAAAGGATGCCAAGGGTTAGGTTTGGGTATGCGCATGTGGCCAACAATTTATATGAGGAGTGGAAGATGTATGCCGTAGCAGGCAGCGCCAATCCAACAATTTTCAGTGAAGGGAACTACTTCATAGCTGCTGAGAATCCTTTTGCCAAACAGGTTACAAAGAGAGAGGCTGGAGGCAGTTGGAATAATTGGAAATGGAGATCTTTAGGCGATGTGTTAAAAAAATGGTGCTTTTTTTGTTCAATCTGGATATGGAAGCTGCTATCCACTTTACTCTCGAACGCAATTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

201

Amino Acids

22.45

Weight (kDa)

9.05

Isoelectric Point (pI)

27.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 23 - 161 1e-26 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009756)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11920 AT1G30350 AT4G22080 AT4G22090
fragaria_vesca FvH4_3g01680
malus_domestica MD00G1202500.v1.1 MD05G1350000.v1.1 MD10G1324800.v1.1
prunus_persica Prupe.4G017400_v2.0.a1 Prupe.4G020600_v2.0.a1
pyrus_communis pycom10g27510
rosa_chinensis RchiOBHm_Chr5g0002531
rosa_laevigata RLG00000031037
rosa_multiflora Rmu_sc0010731.1_g000004
rosa_roxburghii Rroxscaffold_1G00073370
rosa_rugosa Rorug04G0396700
rosa_samantha Rh5AG020100 Rh5BG022800 Rh5CG022100 Rh5DG021200
rosa_wichuraiana Rw5G001850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 342
AcoI YGGCCR 1 cut(s) 348
AcyI GRCGYC 1 cut(s) 81
AfiI CCNNNNNNNGG 2 cut(s) 19, 295
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 3 cut(s) 286, 311, 552
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 3 cut(s) 134, 437, 568
AluI AGCT 3 cut(s) 134, 437, 568
Alw21I GWGCWC 1 cut(s) 41
Alw26I GTCTC 1 cut(s) 72
AoxI GGCC 3 cut(s) 148, 301, 348
ApeKI GCWGC 4 cut(s) 226, 393, 437, 568
Asp700I GAANNNNTTC 1 cut(s) 428
AspLEI GCGC 2 cut(s) 343, 398
AsuHPI GGTGA 2 cut(s) 154, 175
BalI TGGCCA 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 4 cut(s) 213, 405, 424, 555
BccI CCATC 2 cut(s) 140, 194
BceAI ACGGC 2 cut(s) 164, 368
BciT130I CCWGG 1 cut(s) 14
BcoDI GTCTC 1 cut(s) 72
BfaI CTAG 2 cut(s) 65, 131
BfoI RGCGCY 1 cut(s) 399
BglII AGATCT 1 cut(s) 509
BisI GCNGC 4 cut(s) 227, 394, 438, 569
BlsI GCNGC 4 cut(s) 228, 395, 439, 570
Bme1390I CCNGG 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 14
BmsI GCATC 2 cut(s) 213, 306
BpmI CTGGAG 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 13, 320
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 295
Bse3DI GCAATG 1 cut(s) 121
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 13, 320
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 19, 295
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 2 cut(s) 221, 432
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 4 cut(s) 213, 405, 424, 555
BshFI GGCC 3 cut(s) 150, 303, 350
BsiHKAI GWGCWC 1 cut(s) 41
BslI CCNNNNNNNGG 2 cut(s) 19, 295
BsmAI GTCTC 1 cut(s) 72
BsmBI CGTCTC 1 cut(s) 72
BsnI GGCC 3 cut(s) 150, 303, 350
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 1 cut(s) 509
BspANI GGCC 3 cut(s) 150, 303, 350
BspCNI CTCAG 2 cut(s) 220, 433
BsrDI GCAATG 1 cut(s) 121
BssECI CCNNGG 2 cut(s) 13, 320
BssMI GATC 1 cut(s) 509
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 301, 391
BstDEI CTNAG 2 cut(s) 207, 441
BstF5I GGATG 1 cut(s) 321
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 2 cut(s) 343, 398
BstKTI GATC 1 cut(s) 512
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 1 cut(s) 509
BstMWI GCNNNNNNNGC 4 cut(s) 147, 176, 347, 395
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 3 cut(s) 53, 110, 347
BstSCI CCNGG 1 cut(s) 12
BstV1I GCAGC 4 cut(s) 213, 405, 424, 555
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BsuRI GGCC 3 cut(s) 150, 303, 350
BtgZI GCGATG 2 cut(s) 87, 534
BtsCI GGATG 1 cut(s) 321
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 2 cut(s) 174, 421
Cac8I GCNNGC 2 cut(s) 301, 391
CfoI GCGC 2 cut(s) 343, 398
CseI GACGC 1 cut(s) 89
CviAII CATG 5 cut(s) 50, 107, 167, 212, 344
DdeI CTNAG 2 cut(s) 207, 441
DpnI GATC 1 cut(s) 511
DpnII GATC 1 cut(s) 509
EaeI YGGCCR 1 cut(s) 348
Eco130I CCWWGG 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 320
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 5 cut(s) 53, 110, 170, 215, 347
FatI CATG 5 cut(s) 49, 106, 166, 211, 343
Fnu4HI GCNGC 4 cut(s) 227, 394, 438, 569
FokI GGATG 1 cut(s) 328
Fsp4HI GCNGC 4 cut(s) 227, 394, 438, 569
FspAI RTGCGCAY 1 cut(s) 342
FspBI CTAG 2 cut(s) 65, 131
FspI TGCGCA 1 cut(s) 342
GlaI GCGC 2 cut(s) 342, 397
GluI GCNGC 4 cut(s) 227, 394, 438, 569
GsuI CTGGAG 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 3 cut(s) 150, 303, 350
HgaI GACGC 1 cut(s) 89
HhaI GCGC 2 cut(s) 343, 398
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 5 cut(s) 53, 110, 170, 215, 347
Hin6I GCGC 2 cut(s) 341, 396
HinP1I GCGC 2 cut(s) 341, 396
HinfI GANTC 2 cut(s) 270, 445
HphI GGTGA 2 cut(s) 154, 175
Hpy188III TCNNGA 2 cut(s) 556, 586
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 3 cut(s) 12, 292, 413
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 1 cut(s) 61
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 176, 347, 395
HpyF3I CTNAG 2 cut(s) 207, 441
HpySE526I ACGT 1 cut(s) 61
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 5 cut(s) 53, 110, 170, 215, 347
HspAI GCGC 2 cut(s) 341, 396
Kzo9I GATC 1 cut(s) 509
LmnI GCTCC 2 cut(s) 36, 69
LpnPI CCDG 6 cut(s) 26, 285, 375, 446, 465, 541
Lsp1109I GCAGC 4 cut(s) 213, 405, 424, 555
LweI GCATC 2 cut(s) 213, 306
MaeI CTAG 2 cut(s) 65, 131
MaeII ACGT 1 cut(s) 61
MaeIII GTNAC 5 cut(s) 160, 181, 233, 271, 463
MalI GATC 1 cut(s) 511
MboI GATC 1 cut(s) 509
MboII GAAGA 2 cut(s) 266, 385
MfeI CAATTG 1 cut(s) 594
MflI RGATCY 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 41
MlsI TGGCCA 1 cut(s) 350
MluCI AATT 5 cut(s) 244, 355, 408, 496, 594
MluNI TGGCCA 1 cut(s) 350
MlyI GAGTC 1 cut(s) 279
MmeI TCCRAC 2 cut(s) 428, 470
MnlI CCTC 4 cut(s) 161, 358, 469, 476
Mox20I TGGCCA 1 cut(s) 350
MroXI GAANNNNTTC 1 cut(s) 428
MscI TGGCCA 1 cut(s) 350
MseI TTAA 2 cut(s) 527, 601
Msp20I TGGCCA 1 cut(s) 350
MspR9I CCNGG 1 cut(s) 14
MunI CAATTG 1 cut(s) 594
MvaI CCWGG 1 cut(s) 14
MwoI GCNNNNNNNGC 4 cut(s) 147, 176, 347, 395
NdeII GATC 1 cut(s) 509
NlaIII CATG 5 cut(s) 53, 110, 170, 215, 347
NmuCI GTSAC 4 cut(s) 160, 181, 233, 271
NsbI TGCGCA 1 cut(s) 342
NspI RCATGY 3 cut(s) 53, 110, 347
PciI ACATGT 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 445
PkrI GCNGC 4 cut(s) 228, 395, 439, 570
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
PscI ACATGT 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PsuI RGATCY 1 cut(s) 509
SaqAI TTAA 2 cut(s) 527, 601
SatI GCNGC 4 cut(s) 227, 394, 438, 569
Sau3AI GATC 1 cut(s) 509
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 1 cut(s) 41
SetI ASST 6 cut(s) 64, 136, 332, 439, 465, 570
SfaNI GCATC 2 cut(s) 213, 306
Sse9I AATT 5 cut(s) 244, 355, 408, 496, 594
SspMI CTAG 2 cut(s) 65, 131
StyD4I CCNGG 1 cut(s) 12
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 587
TasI AATT 5 cut(s) 244, 355, 408, 496, 594
TfiI GAWTC 1 cut(s) 445
Tru1I TTAA 2 cut(s) 527, 601
Tru9I TTAA 2 cut(s) 527, 601
TscAI CASTG 2 cut(s) 181, 421
TseFI GTSAC 4 cut(s) 160, 181, 233, 271
TseI GCWGC 4 cut(s) 226, 393, 437, 568
Tsp45I GTSAC 4 cut(s) 160, 181, 233, 271
TspDTI ATGAA 2 cut(s) 90, 421
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 2 cut(s) 181, 421
XceI RCATGY 3 cut(s) 53, 110, 347
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 2 cut(s) 65, 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.