pycom111g00040

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0009269
Physical Location & Seq
Forward (+)
57129 .. 58193
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00040.1

Sequence Viewer

Length: 1065 bp
ATGGGAGCGAGTAGAAATAGCGACCCGAATCAGGACGGGTCAGATGAGCAGCAGAAACGATCGGAGATCTACACCTTCGATGCGCCGTGGCACATCTACGCCATGAACTGGAGCGTCCGTCGGGACAAGAAGTACCGGCTAGCCATCGCCAGCCTCCTCGAGCAGTACCCAAACCGAGTGGAGATCGTGCAGCTGGACGACTCAAACGGGGAGATCCGGTCGGACCCCAACCTGTCCTTCGAGCACCCGTACCCGCCAACCAAGACCATCTTCATCCCGGACAAGGAGTGCCAGAAGCCAGACCTGCTGGCCACCTCCAGCGATTTCCTCCGCGTGTGGCGCATCTCAGGGGATGAGGACGACTCGGACTCCTCGTCGTCTGTGGAGCTCAAGTCCCTCCTCAACGGCAACAAGAACTCCGAGTACTGCGGGCCCATCACCTCCTTCGACTGGAACGAGGCGGAACCCAAGCGCATCGGCACCTCCTCCATCGACACCACGTGCACCATCTGGGACATCGAGCGCGAGGCCGTGGACACCCAGCTCATCGCCCACGACAAGGAGGTGTACGACATCGCTTGGGGCGGCGTCGGTGTATTCGCCTCCGTCTCGGCCGACGGCTCCGTGAGAGTGTTTGACCTGCGCGACAAGGAGCACTCCACCATCATTTACGAGAGCTCGGAGCCGGACACCCCCTTGGTCCGCCTGGGATGGAACAAGCAGGACCCCAGGTACATGGCAACCATCATCATGGACAGCGCCAAGGTGGTCGTCCTCGACATCCGCTTCCCGACCCTCCCCGTGGTTGAGTTGCAGAGGCACCAGTCCAGCGTCAACGCCATTGCCTGGGCCCCACACAGCTCTTGCCACATCTGCACCGCCGGTGATGACTCCCAGGCCCTCATCTGGGACCTCTCCTCCATGGGCCAGCCAGTGGAGGGCGGCCTTGATCCCATTCTGGCCTACACGGCTGGGGCCGAAATCGAGCAGCTGCAGTGGTCATCTTCCCAGCCTGATTGGGTTGCAATTGCCTTCTCTACCAAGCTTCAGATACTCAGGGTATGA

Protein Analysis

355

Amino Acids

39.7

Weight (kDa)

4.61

Isoelectric Point (pI)

55.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 141 - 338 3.1e-18 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 145 - 308 2.8e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 146 - 228 2.2e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 146 - 227 3.9e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 146 - 225 9e-08 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 146 - 222 7.6e-07 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 157 - 218 6.2e-06 G protein beta WD-40 repeat protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 312, 648
AccB1I GGYRCC 2 cut(s) 479, 819
AccB7I CCANNNNNTGG 3 cut(s) 108, 846, 934
AccII CGCG 3 cut(s) 333, 525, 645
AciI CCGC 9 cut(s) 254, 331, 429, 461, 585, 703, 784, 879, 942
AclWI GGATC 2 cut(s) 208, 944
AcoI YGGCCR 2 cut(s) 309, 612
AcuI CTGAAG 1 cut(s) 1031
AcvI CACGTG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 588
AdeI CACNNNGTG 1 cut(s) 501
AfaI GTAC 6 cut(s) 134, 167, 251, 425, 569, 734
AhdI GACNNNNNGTC 1 cut(s) 373
AjnI CCWGG 4 cut(s) 705, 728, 845, 894
AjuI GAANNNNNNNTTGG 4 cut(s) 221, 253, 254, 286
AluBI AGCT 7 cut(s) 193, 388, 544, 678, 861, 991, 1045
AluI AGCT 7 cut(s) 193, 388, 544, 678, 861, 991, 1045
Alw21I GWGCWC 5 cut(s) 246, 390, 506, 657, 680
Alw26I GTCTC 1 cut(s) 613
Alw44I GTGCAC 1 cut(s) 502
AlwI GGATC 2 cut(s) 208, 944
Ama87I CYCGRG 1 cut(s) 158
ApaI GGGCCC 2 cut(s) 435, 853
ApaLI GTGCAC 1 cut(s) 502
ApeKI GCWGC 4 cut(s) 49, 190, 988, 991
AspLEI GCGC 6 cut(s) 85, 342, 474, 525, 645, 761
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 2 cut(s) 430, 896
AsuNHI GCTAGC 1 cut(s) 139
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 4 cut(s) 223, 700, 724, 910
BaeGI GKGCMC 3 cut(s) 435, 506, 853
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 2 cut(s) 479, 819
BanII GRGCYC 4 cut(s) 390, 435, 680, 853
BbrPI CACGTG 1 cut(s) 501
Bbv12I GWGCWC 5 cut(s) 246, 390, 506, 657, 680
BbvI GCAGC 4 cut(s) 61, 202, 978, 1000
BccI CCATC 8 cut(s) 152, 275, 443, 497, 515, 671, 705, 752
BceAI ACGGC 5 cut(s) 70, 421, 515, 634, 984
BciT130I CCWGG 4 cut(s) 707, 730, 847, 896
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 613
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 992
BfoI RGCGCY 1 cut(s) 762
BfuAI ACCTGC 2 cut(s) 312, 648
BglI GCCNNNNNGGC 1 cut(s) 968
BglII AGATCT 1 cut(s) 66
BisI GCNGC 6 cut(s) 50, 191, 586, 943, 989, 992
BlsI GCNGC 6 cut(s) 51, 192, 587, 944, 990, 993
BmcAI AGTACT 1 cut(s) 425
Bme1390I CCNGG 5 cut(s) 278, 707, 730, 847, 896
Bme18I GGWCC 4 cut(s) 223, 700, 724, 910
BmeRI GACNNNNNGTC 1 cut(s) 373
BmeT110I CYCGRG 1 cut(s) 158
BmrFI CCNGG 5 cut(s) 278, 707, 730, 847, 896
BmsI GCATC 3 cut(s) 70, 351, 483
BmtI GCTAGC 1 cut(s) 143
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 2 cut(s) 130, 301
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 588
BsaWI WCCGGW 1 cut(s) 216
Bse118I RCCGGY 2 cut(s) 135, 881
Bse1I ACTGG 4 cut(s) 113, 455, 823, 932
Bse3DI GCAATG 1 cut(s) 840
BseBI CCWGG 4 cut(s) 707, 730, 847, 896
BseGI GGATG 4 cut(s) 273, 358, 716, 780
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 4 cut(s) 113, 455, 823, 932
BseRI GAGGAG 5 cut(s) 146, 361, 389, 475, 907
BseSI GKGCMC 3 cut(s) 435, 506, 853
BseX3I CGGCCG 1 cut(s) 612
BseXI GCAGC 4 cut(s) 61, 202, 978, 1000
BseYI CCCAGC 3 cut(s) 540, 971, 1008
BsgI GTGCAG 2 cut(s) 209, 859
Bsh1236I CGCG 3 cut(s) 333, 525, 645
Bsh1285I CGRYCG 3 cut(s) 62, 221, 615
BshNI GGYRCC 2 cut(s) 479, 819
BsiEI CGRYCG 3 cut(s) 62, 221, 615
BsiHKAI GWGCWC 5 cut(s) 246, 390, 506, 657, 680
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 5 cut(s) 136, 217, 278, 686, 882
BslFI GGGAC 4 cut(s) 137, 379, 527, 923
BsmAI GTCTC 1 cut(s) 613
BsmBI CGTCTC 1 cut(s) 613
BsmFI GGGAC 4 cut(s) 137, 379, 527, 923
BsoBI CYCGRG 1 cut(s) 158
Bsp120I GGGCCC 2 cut(s) 431, 849
Bsp1286I GDGCHC 7 cut(s) 246, 390, 435, 506, 657, 680, 853
Bsp143I GATC 5 cut(s) 59, 66, 183, 213, 949
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 9 cut(s) 254, 331, 429, 461, 585, 703, 784, 879, 942
BspCNI CTCAG 1 cut(s) 359
BspFNI CGCG 3 cut(s) 333, 525, 645
BspMAI CTGCAG 1 cut(s) 996
BspMI ACCTGC 2 cut(s) 312, 648
BspOI GCTAGC 1 cut(s) 143
BspPI GGATC 2 cut(s) 208, 944
BspT107I GGYRCC 2 cut(s) 479, 819
BsrDI GCAATG 1 cut(s) 840
BsrFI RCCGGY 2 cut(s) 135, 881
BsrI ACTGG 4 cut(s) 113, 455, 823, 932
BssAI RCCGGY 2 cut(s) 135, 881
BssMI GATC 5 cut(s) 59, 66, 183, 213, 949
BssNI GRCGYC 1 cut(s) 588
BssT1I CCWWGG 3 cut(s) 696, 762, 921
Bst2UI CCWGG 4 cut(s) 707, 730, 847, 896
BstACI GRCGYC 1 cut(s) 588
BstBAI YACGTR 1 cut(s) 501
BstC8I GCNNGC 5 cut(s) 141, 151, 309, 431, 929
BstDEI CTNAG 2 cut(s) 346, 1055
BstDSI CCRYGG 4 cut(s) 86, 531, 801, 921
BstF5I GGATG 4 cut(s) 273, 358, 716, 780
BstFNI CGCG 3 cut(s) 333, 525, 645
BstH2I RGCGCY 1 cut(s) 762
BstHHI GCGC 6 cut(s) 85, 342, 474, 525, 645, 761
BstKTI GATC 5 cut(s) 62, 69, 186, 216, 952
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 5 cut(s) 59, 66, 183, 213, 949
BstMCI CGRYCG 3 cut(s) 62, 221, 615
BstMWI GCNNNNNNNGC 4 cut(s) 304, 339, 873, 968
BstNI CCWGG 4 cut(s) 707, 730, 847, 896
BstSCI CCNGG 5 cut(s) 276, 705, 728, 845, 894
BstSFI CTRYAG 1 cut(s) 992
BstSLI GKGCMC 3 cut(s) 435, 506, 853
BstUI CGCG 3 cut(s) 333, 525, 645
BstV1I GCAGC 4 cut(s) 61, 202, 978, 1000
BstX2I RGATCY 2 cut(s) 66, 213
BstXI CCANNNNNNTGG 2 cut(s) 736, 751
BstYI RGATCY 2 cut(s) 66, 213
BstZI CGGCCG 1 cut(s) 612
BtgI CCRYGG 4 cut(s) 86, 531, 801, 921
BtgZI GCGATG 3 cut(s) 130, 532, 559
BtsCI GGATG 4 cut(s) 273, 358, 716, 780
BtsI GCAGTG 1 cut(s) 1001
BtsIMutI CAGTG 2 cut(s) 939, 1001
BveI ACCTGC 2 cut(s) 312, 648
Cac8I GCNNGC 5 cut(s) 141, 151, 309, 431, 929
CfoI GCGC 6 cut(s) 85, 342, 474, 525, 645, 761
Cfr10I RCCGGY 2 cut(s) 135, 881
CseI GACGC 3 cut(s) 103, 577, 820
Csp6I GTAC 6 cut(s) 133, 166, 250, 424, 568, 733
CspCI CAANNNNNGTGG 2 cut(s) 159, 194
CviAII CATG 4 cut(s) 103, 736, 751, 922
CviQI GTAC 6 cut(s) 133, 166, 250, 424, 568, 733
DdeI CTNAG 2 cut(s) 346, 1055
DpnI GATC 5 cut(s) 61, 68, 185, 215, 951
DpnII GATC 5 cut(s) 59, 66, 183, 213, 949
DraIII CACNNNGTG 1 cut(s) 501
DriI GACNNNNNGTC 1 cut(s) 373
EaeI YGGCCR 2 cut(s) 309, 612
EagI CGGCCG 1 cut(s) 612
Eam1105I GACNNNNNGTC 1 cut(s) 373
EciI GGCGGA 2 cut(s) 476, 692
Ecl136II GAGCTC 2 cut(s) 388, 678
EclXI CGGCCG 1 cut(s) 612
Eco130I CCWWGG 3 cut(s) 696, 762, 921
Eco24I GRGCYC 4 cut(s) 390, 435, 680, 853
Eco47I GGWCC 4 cut(s) 223, 700, 724, 910
Eco52I CGGCCG 1 cut(s) 612
Eco53kI GAGCTC 2 cut(s) 388, 678
Eco57I CTGAAG 1 cut(s) 1031
Eco72I CACGTG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 2 cut(s) 388, 678
EcoO109I RGGNCCY 4 cut(s) 724, 850, 898, 910
EcoRII CCWGG 4 cut(s) 705, 728, 845, 894
EcoT14I CCWWGG 3 cut(s) 696, 762, 921
EcoT38I GRGCYC 4 cut(s) 390, 435, 680, 853
ErhI CCWWGG 3 cut(s) 696, 762, 921
Esp3I CGTCTC 1 cut(s) 613
FaeI CATG 4 cut(s) 106, 739, 754, 925
FaiI YATR 5 cut(s) 104, 737, 752, 923, 1063
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 4 cut(s) 137, 379, 527, 923
FatI CATG 4 cut(s) 102, 735, 750, 921
FauI CCCGC 2 cut(s) 261, 422
Fnu4HI GCNGC 6 cut(s) 50, 191, 586, 943, 989, 992
FokI GGATG 4 cut(s) 260, 365, 723, 767
FriOI GRGCYC 4 cut(s) 390, 435, 680, 853
Fsp4HI GCNGC 6 cut(s) 50, 191, 586, 943, 989, 992
FspBI CTAG 1 cut(s) 140
GlaI GCGC 6 cut(s) 84, 341, 473, 524, 644, 760
GluI GCNGC 6 cut(s) 50, 191, 586, 943, 989, 992
GsaI CCCAGC 3 cut(s) 544, 975, 1012
GsuI CTGGAG 2 cut(s) 130, 301
HaeII RGCGCY 1 cut(s) 762
HapII CCGG 5 cut(s) 136, 217, 278, 686, 882
HgaI GACGC 3 cut(s) 103, 577, 820
HhaI GCGC 6 cut(s) 85, 342, 474, 525, 645, 761
Hin1I GRCGYC 1 cut(s) 588
Hin1II CATG 4 cut(s) 106, 739, 754, 925
Hin6I GCGC 6 cut(s) 83, 340, 472, 523, 643, 759
HinP1I GCGC 6 cut(s) 83, 340, 472, 523, 643, 759
HincII GTYRAC 1 cut(s) 835
HindII GTYRAC 1 cut(s) 835
HindIII AAGCTT 1 cut(s) 1043
HinfI GANTC 5 cut(s) 28, 200, 362, 368, 890
HpaII CCGG 5 cut(s) 136, 217, 278, 686, 882
HphI GGTGA 2 cut(s) 430, 896
Hpy166II GTNNAC 4 cut(s) 504, 535, 568, 835
Hpy188I TCNGA 7 cut(s) 43, 64, 223, 367, 421, 682, 1050
Hpy188III TCNNGA 3 cut(s) 32, 122, 790
Hpy8I GTNNAC 4 cut(s) 504, 535, 568, 835
Hpy99I CGWCG 4 cut(s) 123, 379, 593, 620
HpyAV CCTTC 4 cut(s) 85, 247, 454, 1042
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 6 cut(s) 190, 504, 814, 876, 994, 1025
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 339, 873, 968
HpyF3I CTNAG 2 cut(s) 346, 1055
HpySE526I ACGT 1 cut(s) 500
Hsp92I GRCGYC 1 cut(s) 588
Hsp92II CATG 4 cut(s) 106, 739, 754, 925
HspAI GCGC 6 cut(s) 83, 340, 472, 523, 643, 759
Kzo9I GATC 5 cut(s) 59, 66, 183, 213, 949
LmnI GCTCC 6 cut(s) 5, 111, 385, 626, 652, 682
Lsp1109I GCAGC 4 cut(s) 61, 202, 978, 1000
LweI GCATC 3 cut(s) 70, 351, 483
MaeI CTAG 1 cut(s) 140
MaeII ACGT 1 cut(s) 500
MalI GATC 5 cut(s) 61, 68, 185, 215, 951
MboI GATC 5 cut(s) 59, 66, 183, 213, 949
MboII GAAGA 2 cut(s) 262, 996
MfeI CAATTG 1 cut(s) 1026
MflI RGATCY 2 cut(s) 66, 213
MhlI GDGCHC 7 cut(s) 246, 390, 435, 506, 657, 680, 853
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 1 cut(s) 1026
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 4 cut(s) 194, 356, 362, 884
MmeI TCCRAC 1 cut(s) 201
Mox20I TGGCCA 1 cut(s) 311
MscI TGGCCA 1 cut(s) 311
MslI CAYNNNNRTG 1 cut(s) 749
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 2 cut(s) 193, 991
MspI CCGG 5 cut(s) 136, 217, 278, 686, 882
MspR9I CCNGG 5 cut(s) 278, 707, 730, 847, 896
MunI CAATTG 1 cut(s) 1026
MvaI CCWGG 4 cut(s) 707, 730, 847, 896
MvnI CGCG 3 cut(s) 333, 525, 645
MwoI GCNNNNNNNGC 4 cut(s) 304, 339, 873, 968
NciI CCSGG 1 cut(s) 278
NcoI CCATGG 1 cut(s) 921
NdeII GATC 5 cut(s) 59, 66, 183, 213, 949
NheI GCTAGC 1 cut(s) 139
NlaIII CATG 4 cut(s) 106, 739, 754, 925
NmeAIII GCCGAG 1 cut(s) 590
PaeR7I CTCGAG 1 cut(s) 158
PcsI WCGNNNNNNNCGW 2 cut(s) 371, 453
PfeI GAWTC 1 cut(s) 28
PflMI CCANNNNNTGG 3 cut(s) 108, 846, 934
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 6 cut(s) 51, 192, 587, 944, 990, 993
Ple19I CGATCG 1 cut(s) 62
PleI GAGTC 4 cut(s) 194, 356, 362, 884
PmaCI CACGTG 1 cut(s) 501
PmlI CACGTG 1 cut(s) 501
PpsI GAGTC 4 cut(s) 194, 356, 362, 884
Ppu21I YACGTR 1 cut(s) 501
PpuMI RGGWCCY 2 cut(s) 724, 910
Psp124BI GAGCTC 2 cut(s) 390, 680
Psp5II RGGWCCY 2 cut(s) 724, 910
Psp6I CCWGG 4 cut(s) 705, 728, 845, 894
PspCI CACGTG 1 cut(s) 501
PspFI CCCAGC 3 cut(s) 540, 971, 1008
PspGI CCWGG 4 cut(s) 705, 728, 845, 894
PspOMI GGGCCC 2 cut(s) 431, 849
PspPPI RGGWCCY 2 cut(s) 724, 910
PspXI VCTCGAGB 1 cut(s) 158
PsrI GAACNNNNNNTAC 2 cut(s) 407, 439
PstI CTGCAG 1 cut(s) 996
PsuI RGATCY 2 cut(s) 66, 213
PvuI CGATCG 1 cut(s) 62
PvuII CAGCTG 2 cut(s) 193, 991
RsaI GTAC 6 cut(s) 134, 167, 251, 425, 569, 734
RsaNI GTAC 6 cut(s) 133, 166, 250, 424, 568, 733
RseI CAYNNNNRTG 1 cut(s) 749
SacI GAGCTC 2 cut(s) 390, 680
SatI GCNGC 6 cut(s) 50, 191, 586, 943, 989, 992
Sau3AI GATC 5 cut(s) 59, 66, 183, 213, 949
ScaI AGTACT 1 cut(s) 425
SchI GAGTC 4 cut(s) 194, 356, 362, 884
ScrFI CCNGG 5 cut(s) 278, 707, 730, 847, 896
SduI GDGCHC 7 cut(s) 246, 390, 435, 506, 657, 680, 853
SfaNI GCATC 3 cut(s) 70, 351, 483
SfcI CTRYAG 1 cut(s) 992
Sfr274I CTCGAG 1 cut(s) 158
SgrAI CRCCGGYG 1 cut(s) 881
SinI GGWCC 4 cut(s) 223, 700, 724, 910
SlaI CTCGAG 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 749
SmlI CTYRAG 2 cut(s) 158, 389
SmoI CTYRAG 2 cut(s) 158, 389
Sse9I AATT 1 cut(s) 1026
SsiI CCGC 9 cut(s) 254, 331, 429, 461, 585, 703, 784, 879, 942
SspMI CTAG 1 cut(s) 140
SstI GAGCTC 2 cut(s) 390, 680
StyD4I CCNGG 5 cut(s) 276, 705, 728, 845, 894
StyI CCWWGG 3 cut(s) 696, 762, 921
TaiI ACGT 1 cut(s) 503
TaqI TCGA 8 cut(s) 78, 159, 240, 447, 492, 519, 777, 984
TasI AATT 1 cut(s) 1026
TatI WGTACW 1 cut(s) 423
TauI GCSGC 2 cut(s) 588, 945
TfiI GAWTC 1 cut(s) 28
TscAI CASTG 2 cut(s) 939, 1001
TseI GCWGC 4 cut(s) 49, 190, 988, 991
TspDTI ATGAA 2 cut(s) 119, 262
TspGWI ACGGA 3 cut(s) 107, 595, 613
TspRI CASTG 2 cut(s) 939, 1001
Van91I CCANNNNNTGG 3 cut(s) 108, 846, 934
VneI GTGCAC 1 cut(s) 502
VpaK11BI GGWCC 4 cut(s) 223, 700, 724, 910
XhoI CTCGAG 1 cut(s) 158
XspI CTAG 1 cut(s) 140
ZrmI AGTACT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.