Rh2AG675800

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009269
Physical Location & Seq
Reverse (-)
88755830 .. 88758444
2615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG675800.1

Sequence Viewer

Length: 1047 bp
ATGGGGGCCAGTAGAAGTAGCGATCCAAATCAGGACGGGTCGGATGAGCAGCAGAAACGATCGGAGATCTACACGTACGAGGCGCCGTGGCACATCTACGCCATGAACTGGAGCGTCCGGCGGGACAAGAAGTACCGGCTGGCCATCGCCAGCCTGCTGGAGCAGTACCCGAACCGAGTGGAGATTGTCCAGCTGGACGACTCCAACGGAGAGATCCGGTCCGACCCGAATCTGTGCTTCGAGCACCCGTATCCGCCGACCAAGACCATATTCATCCCGGACAAGGAGTGCCAGAAGCCGGACCTGCTGGCCACATCGAGCGATTTCCTGCGCGTGTGGAGCATCTCGGAAGACTCCTCCTCCGTTGAGCTTAAGTCGGTCCTGAACGGCAACAAGAACTCGGAGTTCTGCGGGCCGCTCACCTCGTTCGATTGGAACGAGGCGGAGCCGAAGCGCATCGGCACCTCCTCCATCGACACCACCTGCACCATCTGGGACATCGAGCGTGAGGCCGTCGACACCCAGCTCATCGCCCACGACAAGGAGGTCTACGACATCGCCTGGGGCGGCGTCGGTGTCTTCGCCTCCGTCTCCGCCGACGGCTCCGTGAGAGTGTTCGACCTGCGTGACAAGGAGCATTCCACCATCATCTACGAGAGCTCCGAGCCCGACACCCCCTTGGTCCGCCTGGGATGGAACAAGCAGGACCCCAGGTACATGGCCACCATCATCATGGATAGCGCCAAGGTCGTCGTCCTCGACATCCGCTTCCCTACGCTTCCGGTCGTCGAGTTGCAGAGGCACCAGGCCAGCGTCAACGCCGTTGCTTGGGCCCCGCACAGCTCTTGCCACATCTGCACTGCCGGGGATGACTCACAGGCCCTCATCTGGGACCTCTCCTCCATGGGCCAGCCCGTCGAGGGTGGCCTCGATCCCATTCTGGCCTACACTGCCGGGGCAGAGATTGAGCAGCTGCAGTGGTCTTCTTCTCAGCCCGATTGGGTTGCAATTGCTTTCTCCACCAAGCTTCAGATACTCAGGGTATGA

Protein Analysis

348

Amino Acids

39.02

Weight (kDa)

4.67

Isoelectric Point (pI)

54.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 102 - 228 5.9e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 109 - 216 2.8e-07 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 136 - 302 2.4e-09 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 137 - 332 8.8e-18 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 141 - 219 3.6e-07 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 142 - 222 3.6e-10 THOC3 beta-propeller domain
WD40_Gbeta PF25391 151 - 212 5.4e-06 G protein beta WD-40 repeat protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 491
AasI GACNNNNNNGTC 1 cut(s) 545
Acc36I ACCTGC 3 cut(s) 312, 491, 630
AccB1I GGYRCC 3 cut(s) 82, 461, 801
AccB7I CCANNNNNTGG 1 cut(s) 108
AccBSI CCGCTC 1 cut(s) 418
AccI GTMKAC 2 cut(s) 516, 549
AccII CGCG 1 cut(s) 333
AclWI GGATC 3 cut(s) 17, 208, 926
AcoI YGGCCR 3 cut(s) 141, 309, 720
AcuI CTGAAG 1 cut(s) 1013
AcyI GRCGYC 2 cut(s) 83, 570
AfaI GTAC 4 cut(s) 77, 134, 167, 716
AfiI CCNNNNNNNGG 8 cut(s) 108, 283, 298, 541, 828, 888, 889, 920
AflII CTTAAG 1 cut(s) 371
AflIII ACRYGT 1 cut(s) 72
AjnI CCWGG 4 cut(s) 560, 687, 710, 804
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 7 cut(s) 193, 370, 526, 660, 843, 973, 1027
AluI AGCT 7 cut(s) 193, 370, 526, 660, 843, 973, 1027
Alw21I GWGCWC 2 cut(s) 246, 662
Alw26I GTCTC 1 cut(s) 595
AlwI GGATC 3 cut(s) 17, 208, 926
ApaI GGGCCC 1 cut(s) 835
ApeKI GCWGC 3 cut(s) 49, 970, 973
AspLEI GCGC 4 cut(s) 85, 333, 456, 743
AsuC2I CCSGG 3 cut(s) 278, 865, 955
AsuHPI GGTGA 1 cut(s) 412
AvaII GGWCC 6 cut(s) 219, 301, 379, 682, 706, 892
BaeGI GKGCMC 1 cut(s) 835
BalI TGGCCA 3 cut(s) 143, 311, 722
BanI GGYRCC 3 cut(s) 82, 461, 801
BanII GRGCYC 3 cut(s) 662, 669, 835
BbsI GAAGAC 3 cut(s) 357, 571, 975
Bbv12I GWGCWC 2 cut(s) 246, 662
BbvI GCAGC 3 cut(s) 61, 960, 982
BccI CCATC 6 cut(s) 152, 479, 497, 653, 687, 734
BceAI ACGGC 5 cut(s) 70, 403, 497, 616, 806
BcgI CGANNNNNNTGC 2 cut(s) 986, 1020
BciT130I CCWGG 4 cut(s) 562, 689, 712, 806
BciVI GTATCC 1 cut(s) 261
BcnI CCSGG 3 cut(s) 278, 865, 955
BcoDI GTCTC 1 cut(s) 595
BfmI CTRYAG 1 cut(s) 974
BfoI RGCGCY 2 cut(s) 86, 744
BfrI CTTAAG 1 cut(s) 371
BfuAI ACCTGC 3 cut(s) 312, 491, 630
BfuI GTATCC 1 cut(s) 261
BglII AGATCT 1 cut(s) 66
BisI GCNGC 5 cut(s) 50, 416, 568, 971, 974
BlsI GCNGC 5 cut(s) 51, 417, 569, 972, 975
Bme1390I CCNGG 7 cut(s) 278, 562, 689, 712, 806, 865, 955
Bme18I GGWCC 6 cut(s) 219, 301, 379, 682, 706, 892
BmrFI CCNGG 7 cut(s) 278, 562, 689, 712, 806, 865, 955
BmsI GCATC 2 cut(s) 351, 465
BpiI GAAGAC 3 cut(s) 357, 571, 975
BpmI CTGGAG 2 cut(s) 130, 179
BpuMI CCSGG 3 cut(s) 278, 865, 955
BsaAI YACGTR 1 cut(s) 75
BsaBI GATNNNNATC 1 cut(s) 27
BsaHI GRCGYC 2 cut(s) 83, 570
BsaJI CCNNGG 9 cut(s) 86, 561, 678, 688, 710, 744, 864, 903, 954
BsaWI WCCGGW 2 cut(s) 216, 781
BsaXI ACNNNNNCTCC 6 cut(s) 344, 374, 644, 674, 884, 914
Bsc4I CCNNNNNNNGG 8 cut(s) 108, 283, 298, 541, 828, 888, 889, 920
Bse118I RCCGGY 1 cut(s) 135
Bse1I ACTGG 2 cut(s) 9, 113
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 4 cut(s) 562, 689, 712, 806
BseDI CCNNGG 9 cut(s) 86, 561, 678, 688, 710, 744, 864, 903, 954
BseGI GGATG 5 cut(s) 49, 273, 698, 762, 874
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 8 cut(s) 108, 283, 298, 541, 828, 888, 889, 920
BseMII CTCAG 1 cut(s) 1004
BseNI ACTGG 2 cut(s) 9, 113
BseRI GAGGAG 4 cut(s) 346, 349, 457, 889
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 3 cut(s) 61, 960, 982
BseYI CCCAGC 1 cut(s) 522
BsgI GTGCAG 2 cut(s) 469, 841
Bsh1236I CGCG 1 cut(s) 333
Bsh1285I CGRYCG 2 cut(s) 62, 786
BshNI GGYRCC 3 cut(s) 82, 461, 801
BsiEI CGRYCG 2 cut(s) 62, 786
BsiHKAI GWGCWC 2 cut(s) 246, 662
BsiSI CCGG 8 cut(s) 118, 136, 217, 278, 299, 782, 864, 954
BsiWI CGTACG 1 cut(s) 75
BslFI GGGAC 3 cut(s) 137, 509, 905
BslI CCNNNNNNNGG 8 cut(s) 108, 283, 298, 541, 828, 888, 889, 920
BsmAI GTCTC 1 cut(s) 595
BsmBI CGTCTC 1 cut(s) 595
BsmFI GGGAC 3 cut(s) 137, 509, 905
BsmI GAATGC 1 cut(s) 637
Bsp120I GGGCCC 1 cut(s) 831
Bsp1286I GDGCHC 4 cut(s) 246, 662, 669, 835
Bsp143I GATC 5 cut(s) 22, 59, 66, 213, 931
Bsp19I CCATGG 1 cut(s) 903
BspCNI CTCAG 1 cut(s) 1003
BspFNI CGCG 1 cut(s) 333
BspMAI CTGCAG 1 cut(s) 978
BspMI ACCTGC 3 cut(s) 312, 491, 630
BspPI GGATC 3 cut(s) 17, 208, 926
BspT107I GGYRCC 3 cut(s) 82, 461, 801
BspTI CTTAAG 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 135
BsrI ACTGG 2 cut(s) 9, 113
BssAI RCCGGY 1 cut(s) 135
BssECI CCNNGG 9 cut(s) 86, 561, 678, 688, 710, 744, 864, 903, 954
BssMI GATC 5 cut(s) 22, 59, 66, 213, 931
BssNI GRCGYC 2 cut(s) 83, 570
BssT1I CCWWGG 3 cut(s) 678, 744, 903
Bst2UI CCWGG 4 cut(s) 562, 689, 712, 806
BstACI GRCGYC 2 cut(s) 83, 570
BstAFI CTTAAG 1 cut(s) 371
BstBAI YACGTR 1 cut(s) 75
BstC8I GCNNGC 7 cut(s) 141, 151, 155, 309, 413, 811, 911
BstDEI CTNAG 2 cut(s) 990, 1037
BstDSI CCRYGG 2 cut(s) 86, 903
BstF5I GGATG 5 cut(s) 49, 273, 698, 762, 874
BstFNI CGCG 1 cut(s) 333
BstH2I RGCGCY 2 cut(s) 86, 744
BstHHI GCGC 4 cut(s) 85, 333, 456, 743
BstKTI GATC 5 cut(s) 25, 62, 69, 216, 934
BstMAI GTCTC 1 cut(s) 595
BstMBI GATC 5 cut(s) 22, 59, 66, 213, 931
BstMCI CGRYCG 2 cut(s) 62, 786
BstMWI GCNNNNNNNGC 4 cut(s) 304, 339, 855, 950
BstNI CCWGG 4 cut(s) 562, 689, 712, 806
BstSCI CCNGG 7 cut(s) 276, 560, 687, 710, 804, 863, 953
BstSFI CTRYAG 1 cut(s) 974
BstSLI GKGCMC 1 cut(s) 835
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 3 cut(s) 61, 960, 982
BstV2I GAAGAC 3 cut(s) 357, 571, 975
BstX2I RGATCY 2 cut(s) 66, 213
BstXI CCANNNNNNTGG 3 cut(s) 157, 718, 733
BstYI RGATCY 2 cut(s) 66, 213
BsuI GTATCC 1 cut(s) 261
BtgI CCRYGG 2 cut(s) 86, 903
BtgZI GCGATG 3 cut(s) 130, 514, 541
BtsCI GGATG 5 cut(s) 49, 273, 698, 762, 874
BtsI GCAGTG 3 cut(s) 858, 948, 983
BtsIMutI CAGTG 3 cut(s) 858, 948, 983
BveI ACCTGC 3 cut(s) 312, 491, 630
Cac8I GCNNGC 7 cut(s) 141, 151, 155, 309, 413, 811, 911
CfoI GCGC 4 cut(s) 85, 333, 456, 743
Cfr10I RCCGGY 1 cut(s) 135
CpoI CGGWCCG 1 cut(s) 219
CseI GACGC 3 cut(s) 103, 559, 802
Csp6I GTAC 4 cut(s) 76, 133, 166, 715
CspI CGGWCCG 1 cut(s) 219
CviAII CATG 4 cut(s) 103, 718, 733, 904
CviQI GTAC 4 cut(s) 76, 133, 166, 715
DdeI CTNAG 2 cut(s) 990, 1037
DinI GGCGCC 1 cut(s) 84
DpnI GATC 5 cut(s) 24, 61, 68, 215, 933
DpnII GATC 5 cut(s) 22, 59, 66, 213, 931
DrdI GACNNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 545
EaeI YGGCCR 3 cut(s) 141, 309, 720
EciI GGCGGA 4 cut(s) 243, 458, 583, 674
Ecl136II GAGCTC 1 cut(s) 660
Eco130I CCWWGG 3 cut(s) 678, 744, 903
Eco24I GRGCYC 3 cut(s) 662, 669, 835
Eco47I GGWCC 6 cut(s) 219, 301, 379, 682, 706, 892
Eco53kI GAGCTC 1 cut(s) 660
Eco57I CTGAAG 1 cut(s) 1013
EcoICRI GAGCTC 1 cut(s) 660
EcoO109I RGGNCCY 4 cut(s) 706, 832, 880, 892
EcoRII CCWGG 4 cut(s) 560, 687, 710, 804
EcoT14I CCWWGG 3 cut(s) 678, 744, 903
EcoT38I GRGCYC 3 cut(s) 662, 669, 835
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 3 cut(s) 678, 744, 903
Esp3I CGTCTC 1 cut(s) 595
FaeI CATG 4 cut(s) 106, 721, 736, 907
FaiI YATR 6 cut(s) 104, 269, 719, 734, 905, 1045
FaqI GGGAC 3 cut(s) 137, 509, 905
FatI CATG 4 cut(s) 102, 717, 732, 903
FauI CCCGC 3 cut(s) 114, 404, 843
FblI GTMKAC 2 cut(s) 516, 549
Fnu4HI GCNGC 5 cut(s) 50, 416, 568, 971, 974
FokI GGATG 5 cut(s) 56, 260, 705, 749, 881
FriOI GRGCYC 3 cut(s) 662, 669, 835
Fsp4HI GCNGC 5 cut(s) 50, 416, 568, 971, 974
GlaI GCGC 4 cut(s) 84, 332, 455, 742
GluI GCNGC 5 cut(s) 50, 416, 568, 971, 974
GsaI CCCAGC 1 cut(s) 526
GsuI CTGGAG 2 cut(s) 130, 179
HaeII RGCGCY 2 cut(s) 86, 744
HapII CCGG 8 cut(s) 118, 136, 217, 278, 299, 782, 864, 954
HgaI GACGC 3 cut(s) 103, 559, 802
HhaI GCGC 4 cut(s) 85, 333, 456, 743
Hin1I GRCGYC 2 cut(s) 83, 570
Hin1II CATG 4 cut(s) 106, 721, 736, 907
Hin6I GCGC 4 cut(s) 83, 331, 454, 741
HinP1I GCGC 4 cut(s) 83, 331, 454, 741
HincII GTYRAC 2 cut(s) 517, 817
HindII GTYRAC 2 cut(s) 517, 817
HindIII AAGCTT 1 cut(s) 1025
HinfI GANTC 4 cut(s) 200, 229, 353, 872
HpaII CCGG 8 cut(s) 118, 136, 217, 278, 299, 782, 864, 954
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 3 cut(s) 517, 550, 817
Hpy188I TCNGA 7 cut(s) 43, 64, 223, 349, 403, 664, 1032
Hpy188III TCNNGA 2 cut(s) 32, 382
Hpy8I GTNNAC 3 cut(s) 517, 550, 817
Hpy99I CGWCG 6 cut(s) 518, 575, 602, 755, 791, 920
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 5 cut(s) 486, 796, 858, 976, 1007
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 339, 855, 950
HpyF3I CTNAG 2 cut(s) 990, 1037
HpySE526I ACGT 1 cut(s) 74
Hsp92I GRCGYC 2 cut(s) 83, 570
Hsp92II CATG 4 cut(s) 106, 721, 736, 907
HspAI GCGC 4 cut(s) 83, 331, 454, 741
KasI GGCGCC 1 cut(s) 82
Kzo9I GATC 5 cut(s) 22, 59, 66, 213, 931
LmnI GCTCC 7 cut(s) 111, 160, 339, 445, 608, 634, 665
Lsp1109I GCAGC 3 cut(s) 61, 960, 982
LweI GCATC 2 cut(s) 351, 465
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 1 cut(s) 626
MalI GATC 5 cut(s) 24, 61, 68, 215, 933
MbiI CCGCTC 1 cut(s) 418
MboI GATC 5 cut(s) 22, 59, 66, 213, 931
MboII GAAGA 4 cut(s) 362, 571, 975, 978
MfeI CAATTG 1 cut(s) 1008
MflI RGATCY 2 cut(s) 66, 213
MhlI GDGCHC 4 cut(s) 246, 662, 669, 835
MlsI TGGCCA 3 cut(s) 143, 311, 722
MluCI AATT 1 cut(s) 1008
MluNI TGGCCA 3 cut(s) 143, 311, 722
Mly113I GGCGCC 1 cut(s) 83
MlyI GAGTC 3 cut(s) 194, 347, 866
MmeI TCCRAC 3 cut(s) 21, 228, 246
Mox20I TGGCCA 3 cut(s) 143, 311, 722
MscI TGGCCA 3 cut(s) 143, 311, 722
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 731
Msp20I TGGCCA 3 cut(s) 143, 311, 722
MspA1I CMGCKG 2 cut(s) 193, 973
MspCI CTTAAG 1 cut(s) 371
MspI CCGG 8 cut(s) 118, 136, 217, 278, 299, 782, 864, 954
MspR9I CCNGG 7 cut(s) 278, 562, 689, 712, 806, 865, 955
MunI CAATTG 1 cut(s) 1008
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 4 cut(s) 562, 689, 712, 806
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 4 cut(s) 304, 339, 855, 950
NarI GGCGCC 1 cut(s) 83
NciI CCSGG 3 cut(s) 278, 865, 955
NcoI CCATGG 1 cut(s) 903
NdeII GATC 5 cut(s) 22, 59, 66, 213, 931
NlaIII CATG 4 cut(s) 106, 721, 736, 907
NmuCI GTSAC 1 cut(s) 626
PaqCI CACCTGC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 3 cut(s) 435, 660, 756
PctI GAATGC 1 cut(s) 637
PfeI GAWTC 1 cut(s) 229
Pfl23II CGTACG 1 cut(s) 75
PflMI CCANNNNNTGG 1 cut(s) 108
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 5 cut(s) 51, 417, 569, 972, 975
Ple19I CGATCG 1 cut(s) 62
PleI GAGTC 3 cut(s) 194, 347, 866
PluTI GGCGCC 1 cut(s) 86
PpsI GAGTC 3 cut(s) 194, 347, 866
Ppu21I YACGTR 1 cut(s) 75
PpuMI RGGWCCY 2 cut(s) 706, 892
Psp124BI GAGCTC 1 cut(s) 662
Psp5II RGGWCCY 2 cut(s) 706, 892
Psp6I CCWGG 4 cut(s) 560, 687, 710, 804
PspFI CCCAGC 1 cut(s) 522
PspGI CCWGG 4 cut(s) 560, 687, 710, 804
PspLI CGTACG 1 cut(s) 75
PspOMI GGGCCC 1 cut(s) 831
PspPPI RGGWCCY 2 cut(s) 706, 892
PstI CTGCAG 1 cut(s) 978
PsuI RGATCY 2 cut(s) 66, 213
PvuI CGATCG 1 cut(s) 62
PvuII CAGCTG 2 cut(s) 193, 973
RsaI GTAC 4 cut(s) 77, 134, 167, 716
RsaNI GTAC 4 cut(s) 76, 133, 166, 715
RseI CAYNNNNRTG 1 cut(s) 731
Rsr2I CGGWCCG 1 cut(s) 219
RsrII CGGWCCG 1 cut(s) 219
SacI GAGCTC 1 cut(s) 662
SalI GTCGAC 1 cut(s) 515
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 5 cut(s) 50, 416, 568, 971, 974
Sau3AI GATC 5 cut(s) 22, 59, 66, 213, 931
SchI GAGTC 3 cut(s) 194, 347, 866
ScrFI CCNGG 7 cut(s) 278, 562, 689, 712, 806, 865, 955
SduI GDGCHC 4 cut(s) 246, 662, 669, 835
SfaNI GCATC 2 cut(s) 351, 465
SfcI CTRYAG 1 cut(s) 974
SfoI GGCGCC 1 cut(s) 84
SinI GGWCC 6 cut(s) 219, 301, 379, 682, 706, 892
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 1 cut(s) 1008
SspDI GGCGCC 1 cut(s) 82
SstI GAGCTC 1 cut(s) 662
StyD4I CCNGG 7 cut(s) 276, 560, 687, 710, 804, 863, 953
StyI CCWWGG 3 cut(s) 678, 744, 903
TaiI ACGT 1 cut(s) 77
TaqII GACCGA 1 cut(s) 367
TasI AATT 1 cut(s) 1008
TauI GCSGC 2 cut(s) 418, 570
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 3 cut(s) 865, 955, 983
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 3 cut(s) 49, 970, 973
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 2 cut(s) 119, 262
TspGWI ACGGA 4 cut(s) 222, 352, 577, 595
TspRI CASTG 3 cut(s) 865, 955, 983
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 371
VpaK11BI GGWCC 6 cut(s) 219, 301, 379, 682, 706, 892
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XmiI GTMKAC 2 cut(s) 516, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.