pycom111g00970

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0012812
Physical Location & Seq
Forward (+)
628077 .. 629606
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00970.1

Sequence Viewer

Length: 1530 bp
ATGTCTAAGACGGAAATATCCATGTTGCTCTTCACACATCCTACATTCATTGTTTTCTTAGCAATCATTCTCTATGTTTTGCATGTGGTTTTTTCGAGATTCCGGCGAGTCTATGAGTTTTCCTACCAAGGCCCGCCGAGGTATCCCGTCATCGGGTGCTTCATTTCCTTCTACAACAACCGCAACCGCCTCTTGGACTGGTACACCGATCTCCTTGCAAAATCAACTACCAACACCATCATCGTGGACCGCTTCGGCGCACGGCGGACAGTAGTCACGGCCAACCCGGAAAATGTCGAGTACATGCTCAAAACCAACTTCAACAACTTCCCAAAAGGCAAACCCTTCACCGAAATTCTTGGGGACTTTTTGGGATGTGGTATATTCAATGTGGATGGTGAGGTTTGGCGCACCCAGCGCAAGTTAGCAAGTCATGAGTTCGGCACAAAGTTTCTGCGTGAATGCATGACGAAGACATTGGAGGAAGAAGTGGAAAAGAGTCTATTGCCACTGATGGAGTCGCTGGCGGCGACGGCGCAAGTGGTGGACTTGCAGGAGTTGCTAAGGAGATTCACCTTCAATGTGATTTGTAAGGTGTTTCTGGGAGTTGAGCGGTGCTGCTTGGATCCCTCTCTGCCACATCCGCCGCTTGCAAGGGCTTTTGACACGGCGTCGGAGATTTGCGCAAGGCGCGGAGCAGCTCCCATGTTCATAATTTGGAAGATTAAGAGGTGGCTTGGAGTCGGTTCGGAGCAAAAACTTAGAGTTGCGATTGAAGAAGTTCATGCCTATGTGATGGATATAATCGAGAGGAGGAAGAACGAGCTTGAAGAAGCTGAGACGGATAGTGGAGCTCGCGAAGACCTCCTAACGCGGCTAATAACGGCGGGGTACGAGGAGGAGGTGACGAGGGACATGGTGATAAGCTTTATCATGGCGGGGCGGGACACAACTTCAGCGGCCATGACATGGCTCTTCTGGTTGCTTTCCCGCCAACCAGGTGTGGAGGAAGATGTGGTGAAAGAGATTGAATCCGCCGGAGAAAAGATGTCGGATTTCGAGTTGTTGGAGTTGAGCTTGTTGAAGGCATGCCTATACGAGTCCATGAGGCTTTATCCGCCTGTTGCTTGGGACTCGAAGCATGCCGTAGTTGACGACTTGTTGCCTGACGGGACTCGAGTTCGGGCTGGAGATAGAGTGACGTATTTTCCCTATGGAATGGGTAGAATGGAAGCACTGTGGGGGGAGGACCGGTTGGAATTCAAGCCGGACCGGTGGTTTCTCGAACCGGATATGGAGAGATCAGCTCTGAAAAGGGTATGTCCTTACAGGTTGCCTATTTTCCAGGCAGGTCCAAGGGTTTGTTTGGGGAAGGACATGGCTTTTATTCAGATGAAATATGTGGTGGCTTCGATTCTCAGACGGTTCGAGATCCGATCGGTTGATTCAGGTGAGCCGGTTTTCGTGCCGCGGCTGACGGCTCACATGGCCGGCGGGTTGAAGGTTCTAATCCGCAAGAGAAGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

510

Amino Acids

58.57

Weight (kDa)

7.05

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 43 - 484 1.6e-60 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012812)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01900
fragaria_vesca FvH4_6g53330
malus_domestica MD09G1011400.v1.1 MD17G1011900.v1.1
prunus_persica Prupe.3G305600_v2.0.a1
pyrus_communis pycom111g00970 pycom17g00960
rosa_chinensis RchiOBHm_Chr2g0175491
rosa_laevigata RLG00000022332
rosa_multiflora Rmu_sc0003302.1_g000003 Rmu_sc0021955.1_g000002
rosa_roxburghii Rroxscaffold_2G00077290
rosa_rugosa Rorug02G0585100
rosa_samantha Rh2AG664700 Rh2BG675400 Rh2CG638600 Rh2DG689400
rosa_wichuraiana Rw2G054480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 685
Acc36I ACCTGC 1 cut(s) 1340
AccB7I CCANNNNNTGG 1 cut(s) 969
AccBSI CCGCTC 1 cut(s) 613
AccII CGCG 4 cut(s) 693, 858, 874, 1471
AclWI GGATC 3 cut(s) 620, 633, 1426
AcoI YGGCCR 3 cut(s) 279, 960, 1488
AcsI RAATTY 2 cut(s) 354, 1259
AcuI CTGAAG 1 cut(s) 939
AcyI GRCGYC 1 cut(s) 671
AfaI GTAC 3 cut(s) 203, 302, 893
AfiI CCNNNNNNNGG 4 cut(s) 152, 153, 193, 969
AgeI ACCGGT 2 cut(s) 1251, 1272
AgsI TTSAA 9 cut(s) 322, 388, 580, 776, 830, 1031, 1084, 1264, 1501
AhdI GACNNNNNGTC 1 cut(s) 670
AjnI CCWGG 2 cut(s) 997, 1344
AluBI AGCT 7 cut(s) 701, 826, 836, 854, 927, 1077, 1307
AluI AGCT 7 cut(s) 701, 826, 836, 854, 927, 1077, 1307
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 1 cut(s) 833
AlwI GGATC 3 cut(s) 620, 633, 1426
Ama87I CYCGRG 1 cut(s) 1176
AoxI GGCC 4 cut(s) 130, 279, 960, 1488
ApeKI GCWGC 2 cut(s) 618, 698
ApoI RAATTY 2 cut(s) 354, 1259
AsiGI ACCGGT 2 cut(s) 1251, 1272
Asp700I GAANNNNTTC 1 cut(s) 780
AspLEI GCGC 6 cut(s) 260, 411, 420, 538, 686, 693
AspS9I GGNCC 5 cut(s) 131, 247, 1249, 1270, 1352
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 7 cut(s) 340, 410, 565, 916, 931, 1030, 1463
AvaI CYCGRG 1 cut(s) 1176
AvaII GGWCC 4 cut(s) 247, 1249, 1270, 1352
BamHI GGATCC 1 cut(s) 625
BanII GRGCYC 1 cut(s) 856
BbsI GAAGAC 2 cut(s) 479, 867
Bbv12I GWGCWC 1 cut(s) 856
BbvI GCAGC 2 cut(s) 605, 710
BccI CCATC 4 cut(s) 245, 389, 508, 790
BceAI ACGGC 7 cut(s) 278, 294, 549, 684, 900, 1130, 1494
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 2 cut(s) 999, 1346
BciVI GTATCC 1 cut(s) 153
BcnI CCSGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 1340
BfuI GTATCC 1 cut(s) 153
BisI GCNGC 8 cut(s) 528, 619, 647, 699, 875, 960, 1469, 1472
BlsI GCNGC 8 cut(s) 529, 620, 648, 700, 876, 961, 1470, 1473
Bme1390I CCNGG 3 cut(s) 287, 999, 1346
Bme18I GGWCC 4 cut(s) 247, 1249, 1270, 1352
BmeRI GACNNNNNGTC 1 cut(s) 670
BmeT110I CYCGRG 1 cut(s) 1176
BmgT120I GGNCC 5 cut(s) 131, 247, 1249, 1270, 1352
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 3 cut(s) 287, 999, 1346
BoxI GACNNNNGTC 1 cut(s) 272
BpiI GAAGAC 2 cut(s) 479, 867
BplI GAGNNNNNCTC 2 cut(s) 1291, 1323
BpmI CTGGAG 1 cut(s) 1209
Bpu10I CCTNAGC 1 cut(s) 563
BpuMI CCSGG 1 cut(s) 287
BsaHI GRCGYC 1 cut(s) 671
BsaJI CCNNGG 4 cut(s) 127, 137, 1355, 1469
BsaWI WCCGGW 3 cut(s) 1251, 1272, 1288
BsaXI ACNNNNNCTCC 4 cut(s) 1182, 1212, 1238, 1268
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 153, 193, 969
Bse118I RCCGGY 4 cut(s) 1251, 1272, 1456, 1490
Bse1I ACTGG 1 cut(s) 203
BseBI CCWGG 2 cut(s) 999, 1346
BseDI CCNNGG 4 cut(s) 127, 137, 1355, 1469
BseGI GGATG 4 cut(s) 37, 380, 400, 640
BseLI CCNNNNNNNGG 4 cut(s) 152, 153, 193, 969
BseMII CTCAG 2 cut(s) 828, 1432
BseNI ACTGG 1 cut(s) 203
BseRI GAGGAG 3 cut(s) 826, 911, 914
BseXI GCAGC 2 cut(s) 605, 710
BseYI CCCAGC 1 cut(s) 414
Bsh1236I CGCG 4 cut(s) 693, 858, 874, 1471
Bsh1285I CGRYCG 1 cut(s) 1439
BshFI GGCC 4 cut(s) 132, 281, 962, 1490
BshTI ACCGGT 2 cut(s) 1251, 1272
BsiEI CGRYCG 1 cut(s) 1439
BsiHKAI GWGCWC 1 cut(s) 856
BsiHKCI CYCGRG 1 cut(s) 1176
BsiSI CCGG 9 cut(s) 103, 287, 1038, 1252, 1268, 1273, 1289, 1457, 1491
BslFI GGGAC 5 cut(s) 377, 926, 959, 1145, 1186
BslI CCNNNNNNNGG 4 cut(s) 152, 153, 193, 969
BsmAI GTCTC 1 cut(s) 833
BsmBI CGTCTC 1 cut(s) 833
BsmFI GGGAC 5 cut(s) 377, 926, 959, 1145, 1186
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 4 cut(s) 132, 281, 962, 1490
BsoBI CYCGRG 1 cut(s) 1176
Bsp1286I GDGCHC 1 cut(s) 856
Bsp143I GATC 5 cut(s) 208, 625, 1301, 1431, 1436
Bsp68I TCGCGA 1 cut(s) 858
BspANI GGCC 4 cut(s) 132, 281, 962, 1490
BspCNI CTCAG 2 cut(s) 829, 1431
BspFNI CGCG 4 cut(s) 693, 858, 874, 1471
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 1 cut(s) 627
BspMI ACCTGC 1 cut(s) 1340
BspPI GGATC 3 cut(s) 620, 633, 1426
BspQI GCTCTTC 2 cut(s) 35, 980
BsrBI CCGCTC 1 cut(s) 613
BsrFI RCCGGY 4 cut(s) 1251, 1272, 1456, 1490
BsrI ACTGG 1 cut(s) 203
BssAI RCCGGY 4 cut(s) 1251, 1272, 1456, 1490
BssECI CCNNGG 4 cut(s) 127, 137, 1355, 1469
BssMI GATC 5 cut(s) 208, 625, 1301, 1431, 1436
BssNI GRCGYC 1 cut(s) 671
BssT1I CCWWGG 2 cut(s) 127, 1355
Bst2UI CCWGG 2 cut(s) 999, 1346
Bst4CI ACNGT 3 cut(s) 271, 1239, 1425
Bst6I CTCTTC 2 cut(s) 35, 980
BstACI GRCGYC 1 cut(s) 671
BstAPI GCANNNNNTGC 1 cut(s) 559
BstC8I GCNNGC 7 cut(s) 134, 525, 651, 856, 1090, 1143, 1492
BstDEI CTNAG 6 cut(s) 6, 58, 563, 761, 837, 1418
BstDSI CCRYGG 1 cut(s) 1469
BstF5I GGATG 4 cut(s) 37, 380, 400, 640
BstFNI CGCG 4 cut(s) 693, 858, 874, 1471
BstHHI GCGC 6 cut(s) 260, 411, 420, 538, 686, 693
BstKTI GATC 5 cut(s) 211, 628, 1304, 1434, 1439
BstMAI GTCTC 1 cut(s) 833
BstMBI GATC 5 cut(s) 208, 625, 1301, 1431, 1436
BstMCI CGRYCG 1 cut(s) 1439
BstMWI GCNNNNNNNGC 8 cut(s) 415, 417, 533, 559, 643, 690, 1117, 1487
BstNI CCWGG 2 cut(s) 999, 1346
BstNSI RCATGY 4 cut(s) 86, 307, 1092, 1145
BstPAI GACNNNNGTC 1 cut(s) 272
BstSCI CCNGG 3 cut(s) 285, 997, 1344
BstUI CGCG 4 cut(s) 693, 858, 874, 1471
BstV1I GCAGC 2 cut(s) 605, 710
BstV2I GAAGAC 2 cut(s) 479, 867
BstX2I RGATCY 2 cut(s) 625, 1431
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 2 cut(s) 625, 1431
BsuI GTATCC 1 cut(s) 153
BsuRI GGCC 4 cut(s) 132, 281, 962, 1490
BtgI CCRYGG 1 cut(s) 1469
BtsCI GGATG 4 cut(s) 37, 380, 400, 640
BtsIMutI CAGTG 2 cut(s) 509, 1235
BtuMI TCGCGA 1 cut(s) 858
BveI ACCTGC 1 cut(s) 1340
Cac8I GCNNGC 7 cut(s) 134, 525, 651, 856, 1090, 1143, 1492
CciI TCATGA 1 cut(s) 433
CfoI GCGC 6 cut(s) 260, 411, 420, 538, 686, 693
Cfr10I RCCGGY 4 cut(s) 1251, 1272, 1456, 1490
Cfr13I GGNCC 5 cut(s) 131, 247, 1249, 1270, 1352
Cfr42I CCGCGG 1 cut(s) 1472
CpoI CGGWCCG 1 cut(s) 1270
CseI GACGC 1 cut(s) 660
CsiI ACCWGGT 1 cut(s) 997
Csp6I GTAC 3 cut(s) 202, 301, 892
CspAI ACCGGT 2 cut(s) 1251, 1272
CspI CGGWCCG 1 cut(s) 1270
CviQI GTAC 3 cut(s) 202, 301, 892
DdeI CTNAG 6 cut(s) 6, 58, 563, 761, 837, 1418
DpnI GATC 5 cut(s) 210, 627, 1303, 1433, 1438
DpnII GATC 5 cut(s) 208, 625, 1301, 1431, 1436
DriI GACNNNNNGTC 1 cut(s) 670
EaeI YGGCCR 3 cut(s) 279, 960, 1488
Eam1104I CTCTTC 2 cut(s) 35, 980
Eam1105I GACNNNNNGTC 1 cut(s) 670
EarI CTCTTC 2 cut(s) 35, 980
EciI GGCGGA 4 cut(s) 280, 633, 1024, 1107
Ecl136II GAGCTC 1 cut(s) 854
Eco130I CCWWGG 2 cut(s) 127, 1355
Eco24I GRGCYC 1 cut(s) 856
Eco47I GGWCC 4 cut(s) 247, 1249, 1270, 1352
Eco53kI GAGCTC 1 cut(s) 854
Eco57I CTGAAG 1 cut(s) 939
Eco88I CYCGRG 1 cut(s) 1176
EcoICRI GAGCTC 1 cut(s) 854
EcoRI GAATTC 1 cut(s) 1259
EcoRII CCWGG 2 cut(s) 997, 1344
EcoT14I CCWWGG 2 cut(s) 127, 1355
EcoT22I ATGCAT 1 cut(s) 467
EcoT38I GRGCYC 1 cut(s) 856
ErhI CCWWGG 2 cut(s) 127, 1355
Esp3I CGTCTC 1 cut(s) 833
FaqI GGGAC 5 cut(s) 377, 926, 959, 1145, 1186
FauI CCCGC 6 cut(s) 141, 880, 931, 936, 998, 1487
Fnu4HI GCNGC 8 cut(s) 528, 619, 647, 699, 875, 960, 1469, 1472
FokI GGATG 4 cut(s) 24, 387, 407, 627
FriOI GRGCYC 1 cut(s) 856
Fsp4HI GCNGC 8 cut(s) 528, 619, 647, 699, 875, 960, 1469, 1472
FspI TGCGCA 1 cut(s) 685
GlaI GCGC 6 cut(s) 259, 410, 419, 537, 685, 692
GluI GCNGC 8 cut(s) 528, 619, 647, 699, 875, 960, 1469, 1472
GsaI CCCAGC 1 cut(s) 418
GsuI CTGGAG 1 cut(s) 1209
HaeIII GGCC 4 cut(s) 132, 281, 962, 1490
HapII CCGG 9 cut(s) 103, 287, 1038, 1252, 1268, 1273, 1289, 1457, 1491
HgaI GACGC 1 cut(s) 660
HhaI GCGC 6 cut(s) 260, 411, 420, 538, 686, 693
Hin1I GRCGYC 1 cut(s) 671
Hin6I GCGC 6 cut(s) 258, 409, 418, 536, 684, 691
HinP1I GCGC 6 cut(s) 258, 409, 418, 536, 684, 691
HincII GTYRAC 1 cut(s) 1153
HindII GTYRAC 1 cut(s) 1153
HindIII AAGCTT 1 cut(s) 925
HpaII CCGG 9 cut(s) 103, 287, 1038, 1252, 1268, 1273, 1289, 1457, 1491
HphI GGTGA 7 cut(s) 340, 410, 565, 916, 931, 1030, 1463
Hpy166II GTNNAC 4 cut(s) 204, 247, 547, 1153
Hpy188I TCNGA 7 cut(s) 676, 751, 1054, 1311, 1392, 1421, 1436
Hpy188III TCNNGA 6 cut(s) 96, 434, 808, 857, 1283, 1429
Hpy8I GTNNAC 4 cut(s) 204, 247, 547, 1153
Hpy99I CGWCG 2 cut(s) 535, 676
HpyAV CCTTC 6 cut(s) 178, 355, 586, 1078, 1366, 1495
HpyCH4III ACNGT 3 cut(s) 271, 1239, 1425
HpyCH4IV ACGT 1 cut(s) 1202
HpyCH4V TGCA 5 cut(s) 82, 218, 465, 553, 653
HpyF10VI GCNNNNNNNGC 8 cut(s) 415, 417, 533, 559, 643, 690, 1117, 1487
HpyF3I CTNAG 6 cut(s) 6, 58, 563, 761, 837, 1418
HpySE526I ACGT 1 cut(s) 1202
Hsp92I GRCGYC 1 cut(s) 671
HspAI GCGC 6 cut(s) 258, 409, 418, 536, 684, 691
KroI GCCGGC 1 cut(s) 1490
KroNI GCCGGC 1 cut(s) 1492
KspI CCGCGG 1 cut(s) 1472
Kzo9I GATC 5 cut(s) 208, 625, 1301, 1431, 1436
LguI GCTCTTC 2 cut(s) 35, 980
LmnI GCTCC 4 cut(s) 695, 706, 751, 851
Lsp1109I GCAGC 2 cut(s) 605, 710
MabI ACCWGGT 1 cut(s) 997
MaeII ACGT 1 cut(s) 1202
MaeIII GTNAC 3 cut(s) 274, 904, 1198
MalI GATC 5 cut(s) 210, 627, 1303, 1433, 1438
MbiI CCGCTC 1 cut(s) 613
MboI GATC 5 cut(s) 208, 625, 1301, 1431, 1436
MflI RGATCY 2 cut(s) 625, 1431
MhlI GDGCHC 1 cut(s) 856
MluCI AATT 3 cut(s) 354, 714, 1259
MlyI GAGTC 7 cut(s) 117, 508, 527, 750, 1109, 1127, 1168
MmeI TCCRAC 4 cut(s) 654, 1032, 1047, 1236
Mph1103I ATGCAT 1 cut(s) 467
MroNI GCCGGC 1 cut(s) 1490
MroXI GAANNNNTTC 1 cut(s) 780
MseI TTAA 1 cut(s) 726
MslI CAYNNNNRTG 2 cut(s) 242, 789
MspA1I CMGCKG 2 cut(s) 959, 1471
MspI CCGG 9 cut(s) 103, 287, 1038, 1252, 1268, 1273, 1289, 1457, 1491
MspR9I CCNGG 3 cut(s) 287, 999, 1346
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 2 cut(s) 999, 1346
MvnI CGCG 4 cut(s) 693, 858, 874, 1471
MwoI GCNNNNNNNGC 8 cut(s) 415, 417, 533, 559, 643, 690, 1117, 1487
NaeI GCCGGC 1 cut(s) 1492
NciI CCSGG 1 cut(s) 287
NdeII GATC 5 cut(s) 208, 625, 1301, 1431, 1436
NgoMIV GCCGGC 1 cut(s) 1490
NlaIV GGNNCC 1 cut(s) 627
NmeAIII GCCGAG 1 cut(s) 162
NmuCI GTSAC 3 cut(s) 274, 904, 1198
NruI TCGCGA 1 cut(s) 858
NsbI TGCGCA 1 cut(s) 685
NsiI ATGCAT 1 cut(s) 467
NspI RCATGY 4 cut(s) 86, 307, 1092, 1145
PaeI GCATGC 2 cut(s) 1092, 1145
PaeR7I CTCGAG 1 cut(s) 1176
PagI TCATGA 1 cut(s) 433
PciSI GCTCTTC 2 cut(s) 35, 980
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PctI GAATGC 1 cut(s) 467
PdiI GCCGGC 1 cut(s) 1492
PdmI GAANNNNTTC 1 cut(s) 780
PfeI GAWTC 5 cut(s) 99, 570, 1031, 1414, 1445
PflMI CCANNNNNTGG 1 cut(s) 969
PinAI ACCGGT 2 cut(s) 1251, 1272
PkrI GCNGC 8 cut(s) 529, 620, 648, 700, 876, 961, 1470, 1473
Ple19I CGATCG 1 cut(s) 1439
PleI GAGTC 7 cut(s) 116, 507, 526, 749, 1108, 1127, 1168
PpsI GAGTC 7 cut(s) 116, 507, 526, 749, 1108, 1127, 1168
PshAI GACNNNNGTC 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 856
Psp6I CCWGG 2 cut(s) 997, 1344
PspFI CCCAGC 1 cut(s) 414
PspGI CCWGG 2 cut(s) 997, 1344
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 5 cut(s) 131, 247, 1249, 1270, 1352
PspXI VCTCGAGB 1 cut(s) 1176
PsuI RGATCY 2 cut(s) 625, 1431
PvuI CGATCG 1 cut(s) 1439
RruI TCGCGA 1 cut(s) 858
RsaI GTAC 3 cut(s) 203, 302, 893
RsaNI GTAC 3 cut(s) 202, 301, 892
RseI CAYNNNNRTG 2 cut(s) 242, 789
Rsr2I CGGWCCG 1 cut(s) 1270
RsrII CGGWCCG 1 cut(s) 1270
SacI GAGCTC 1 cut(s) 856
SacII CCGCGG 1 cut(s) 1472
SapI GCTCTTC 2 cut(s) 35, 980
SaqAI TTAA 1 cut(s) 726
SatI GCNGC 8 cut(s) 528, 619, 647, 699, 875, 960, 1469, 1472
Sau3AI GATC 5 cut(s) 208, 625, 1301, 1431, 1436
Sau96I GGNCC 5 cut(s) 131, 247, 1249, 1270, 1352
SchI GAGTC 7 cut(s) 117, 508, 527, 750, 1109, 1127, 1168
ScrFI CCNGG 3 cut(s) 287, 999, 1346
SduI GDGCHC 1 cut(s) 856
SexAI ACCWGGT 1 cut(s) 997
Sfr274I CTCGAG 1 cut(s) 1176
Sfr303I CCGCGG 1 cut(s) 1472
SgrBI CCGCGG 1 cut(s) 1472
SinI GGWCC 4 cut(s) 247, 1249, 1270, 1352
SlaI CTCGAG 1 cut(s) 1176
SmiMI CAYNNNNRTG 2 cut(s) 242, 789
SmlI CTYRAG 1 cut(s) 1176
SmoI CTYRAG 1 cut(s) 1176
SphI GCATGC 2 cut(s) 1092, 1145
Sse9I AATT 3 cut(s) 354, 714, 1259
SstI GAGCTC 1 cut(s) 856
StyD4I CCNGG 3 cut(s) 285, 997, 1344
StyI CCWWGG 2 cut(s) 127, 1355
TaaI ACNGT 3 cut(s) 271, 1239, 1425
TaiI ACGT 1 cut(s) 1205
TaqI TCGA 9 cut(s) 95, 297, 807, 1059, 1136, 1177, 1284, 1412, 1428
TasI AATT 3 cut(s) 354, 714, 1259
TatI WGTACW 1 cut(s) 300
TauI GCSGC 6 cut(s) 530, 649, 877, 962, 1471, 1474
TfiI GAWTC 5 cut(s) 99, 570, 1031, 1414, 1445
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 2 cut(s) 516, 1242
TseFI GTSAC 3 cut(s) 274, 904, 1198
TseI GCWGC 2 cut(s) 618, 698
Tsp45I GTSAC 3 cut(s) 274, 904, 1198
TspDTI ATGAA 5 cut(s) 37, 151, 700, 773, 1409
TspGWI ACGGA 2 cut(s) 26, 857
TspRI CASTG 2 cut(s) 516, 1242
Van91I CCANNNNNTGG 1 cut(s) 969
VpaK11BI GGWCC 4 cut(s) 247, 1249, 1270, 1352
XapI RAATTY 2 cut(s) 354, 1259
XceI RCATGY 4 cut(s) 86, 307, 1092, 1145
XhoI CTCGAG 1 cut(s) 1176
XmnI GAANNNNTTC 1 cut(s) 780
Zsp2I ATGCAT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.