Rroxscaffold_2G00077290

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012812
Physical Location & Seq
Forward (+)
874303 .. 875541
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00077290.1

Sequence Viewer

Length: 1239 bp
ATGCTGAAAACCAACTTCAACAACTTCCCAAAAGGCAAACCCTTCACTGAAATCCTTGGAGACTTTCTTGGATGTGGAATATTCAATGTGGACGGCGAGCTTTGGCTCACGCAGCGCAAGTTAGCCAGCCACGAGTTCAGCACGAAGTCTTTGCGTGAGTACATCATGAACAGCTTGGAGGAAGAAGTCGAAAAAGGCTTATTGCCATTGATGGAGTCACTAGCTATGAAAGGAGAAGTTGTAGACTTGCAGGAGTTGCTGAGGAGATTCGCGTTCAATGTGATTTGCAAGGTGTTTTTGGGGGTTCAGAGGTGCTCTTTGGATCCCTCTTTGCCTGTTCCACAACTCGCAAGGGCTTTCGACACGGCGTCGGAGATCTGTGCAATGCGTGGGGCTGCGCCTGTGTTCATAATATGGAAGATCAAGAGGTGGCTTGGGATTGGTTCGGAGCAGAAACTTAAAGCTGCGGTGGAAGAAGTACATGCCTATGTCATGAACATAATAGAGACGACGAGGCAGAAACTTGATCATGATCAAAGTTCCGGGACTAGTATAGATCAAGATCAAGATCTGCTAACTCGGTTAATAATGGCGGGACATGAAGACGAGGTGACTAGGGACATGGTAATAAGCTTTATAATGGCGGGGAGGGACACAACTTCAGCAGCCATGACGTGGCTCTTCTGGTTGCTTTCCCGCCATCAAACTGTAGAGGAGGATTTGGTGAAAGAGGTTGAAGAGAATCAAAGAACGATGGAATTCGAGTCATTGGATTTCAGATTATTAAAGGCATGCCTTTTCGAATCCATGAGGCTCTTTCCACCTGTGGCTTGGGACTCAAAACATGCCAAAGTTCATGACTTATTGCCGGATGGCACTCATGTCCGGGCCGGAGATAGAGTGACCTATTTTCCCTACGGAATGGGAAGAATGGTGTCGCTGTGGGGGAAGGACCGGCTGGAATTCAAGCCAGACCGGTGGTTTCTCGAACCGGACAAGGAGACGAGATCAGAGCTGAAAAAGGTATGCCCATACAAGTTGCCGATTTTTCAAGCAGGTCCAAGGGTTTGTCTTGGGAAGGAAATGGCTTTTACTCAGATGAAATATGTGATGGCTTCCATTCTCAGGCGGTTCAAAATCAGACCGGTCTATTCCGACGAGCCGGTTTTCGTGCCGCGCCTGACGGCCCACATGGCCGGCGGGTTGAAGGTTCTGGTCCGCAAGAGAGGGACTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

412

Amino Acids

47.32

Weight (kDa)

8.08

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 5 - 386 3.7e-53 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012812)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01900
fragaria_vesca FvH4_6g53330
malus_domestica MD09G1011400.v1.1 MD17G1011900.v1.1
prunus_persica Prupe.3G305600_v2.0.a1
pyrus_communis pycom111g00970 pycom17g00960
rosa_chinensis RchiOBHm_Chr2g0175491
rosa_laevigata RLG00000022332
rosa_multiflora Rmu_sc0003302.1_g000003 Rmu_sc0021955.1_g000002
rosa_roxburghii Rroxscaffold_2G00077290
rosa_rugosa Rorug02G0585100
rosa_samantha Rh2AG664700 Rh2BG675400 Rh2CG638600 Rh2DG689400
rosa_wichuraiana Rw2G054480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
Acc36I ACCTGC 1 cut(s) 1046
AccB7I CCANNNNNTGG 1 cut(s) 675
AccI GTMKAC 1 cut(s) 243
AccII CGCG 2 cut(s) 272, 1177
AciI CCGC 8 cut(s) 467, 593, 644, 697, 1129, 1175, 1200, 1219
AclWI GGATC 2 cut(s) 317, 330
AcoI YGGCCR 1 cut(s) 1194
AcsI RAATTY 2 cut(s) 758, 962
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 2 cut(s) 161, 480
AfiI CCNNNNNNNGG 2 cut(s) 675, 1125
AgeI ACCGGT 2 cut(s) 975, 1144
AgsI TTSAA 8 cut(s) 19, 85, 277, 737, 967, 1052, 1135, 1207
AhdI GACNNNNNGTC 1 cut(s) 367
AhlI ACTAGT 1 cut(s) 548
AjiI CACGTC 1 cut(s) 675
AluBI AGCT 6 cut(s) 100, 174, 224, 464, 633, 1015
AluI AGCT 6 cut(s) 100, 174, 224, 464, 633, 1015
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 3 cut(s) 54, 500, 995
AlwI GGATC 2 cut(s) 317, 330
AoxI GGCC 3 cut(s) 888, 1185, 1194
ApeKI GCWGC 4 cut(s) 112, 395, 464, 665
ApoI RAATTY 2 cut(s) 758, 962
AsiGI ACCGGT 2 cut(s) 975, 1144
AspLEI GCGC 3 cut(s) 117, 400, 1179
AspS9I GGNCC 5 cut(s) 888, 952, 1058, 1186, 1216
AsuC2I CCSGG 2 cut(s) 544, 887
AsuHPI GGTGA 2 cut(s) 622, 736
AsuII TTCGAA 1 cut(s) 801
AvaII GGWCC 3 cut(s) 952, 1058, 1216
BamHI GGATCC 1 cut(s) 322
BauI CACGAG 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 609
Bbv12I GWGCWC 1 cut(s) 317
BbvCI CCTCAGC 1 cut(s) 260
BbvI GCAGC 4 cut(s) 124, 382, 451, 677
BccI CCATC 5 cut(s) 205, 708, 748, 866, 1105
BceAI ACGGC 3 cut(s) 109, 381, 1200
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BclI TGATCA 2 cut(s) 526, 532
BcnI CCSGG 2 cut(s) 544, 887
BcoDI GTCTC 3 cut(s) 54, 500, 995
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 3 cut(s) 221, 549, 615
BfmI CTRYAG 1 cut(s) 708
BfuAI ACCTGC 1 cut(s) 1046
BglI GCCNNNNNGGC 1 cut(s) 1193
BglII AGATCT 2 cut(s) 375, 568
BisI GCNGC 5 cut(s) 113, 396, 465, 666, 1175
BlsI GCNGC 5 cut(s) 114, 397, 466, 667, 1176
Bme1390I CCNGG 2 cut(s) 544, 887
Bme18I GGWCC 3 cut(s) 952, 1058, 1216
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 675
BmgT120I GGNCC 5 cut(s) 888, 952, 1058, 1186, 1216
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 2 cut(s) 544, 887
BpiI GAAGAC 1 cut(s) 609
Bpu10I CCTNAGC 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 801
BpuMI CCSGG 2 cut(s) 544, 887
BsaBI GATNNNNATC 3 cut(s) 531, 561, 567
BsaHI GRCGYC 1 cut(s) 368
BsaJI CCNNGG 2 cut(s) 55, 1061
BsaWI WCCGGW 3 cut(s) 975, 991, 1144
BsaXI ACNNNNNCTCC 4 cut(s) 225, 255, 885, 915
Bsc4I CCNNNNNNNGG 2 cut(s) 675, 1125
Bse118I RCCGGY 5 cut(s) 954, 975, 1144, 1162, 1196
Bse3DI GCAATG 1 cut(s) 390
Bse8I GATNNNNATC 3 cut(s) 531, 561, 567
BseDI CCNNGG 2 cut(s) 55, 1061
BseGI GGATG 2 cut(s) 77, 877
BseJI GATNNNNATC 3 cut(s) 531, 561, 567
BseLI CCNNNNNNNGG 2 cut(s) 675, 1125
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 3 cut(s) 251, 1109, 1138
BseRI GAGGAG 2 cut(s) 277, 728
BseXI GCAGC 4 cut(s) 124, 382, 451, 677
Bsh1236I CGCG 2 cut(s) 272, 1177
BshFI GGCC 3 cut(s) 890, 1187, 1196
BshTI ACCGGT 2 cut(s) 975, 1144
BsiHKAI GWGCWC 1 cut(s) 317
BslFI GGGAC 5 cut(s) 559, 609, 632, 665, 848
BslI CCNNNNNNNGG 2 cut(s) 675, 1125
BsmAI GTCTC 3 cut(s) 54, 500, 995
BsmBI CGTCTC 2 cut(s) 500, 995
BsmFI GGGAC 5 cut(s) 559, 609, 632, 665, 848
BsnI GGCC 3 cut(s) 890, 1187, 1196
Bsp119I TTCGAA 1 cut(s) 801
Bsp1286I GDGCHC 1 cut(s) 317
Bsp143I GATC 9 cut(s) 322, 375, 420, 526, 532, 556, 562, 568, 1007
BspACI CCGC 8 cut(s) 467, 593, 644, 697, 1129, 1175, 1200, 1219
BspANI GGCC 3 cut(s) 890, 1187, 1196
BspCNI CTCAG 3 cut(s) 252, 1108, 1137
BspFNI CGCG 2 cut(s) 272, 1177
BspHI TCATGA 4 cut(s) 165, 492, 529, 856
BspLI GGNNCC 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 1046
BspPI GGATC 2 cut(s) 317, 330
BspQI GCTCTTC 1 cut(s) 686
BspT104I TTCGAA 1 cut(s) 801
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 5 cut(s) 954, 975, 1144, 1162, 1196
BssAI RCCGGY 5 cut(s) 954, 975, 1144, 1162, 1196
BssECI CCNNGG 2 cut(s) 55, 1061
BssMI GATC 9 cut(s) 322, 375, 420, 526, 532, 556, 562, 568, 1007
BssNI GRCGYC 1 cut(s) 368
BssSI CACGAG 1 cut(s) 131
BssT1I CCWWGG 2 cut(s) 55, 1061
Bst2BI CACGAG 1 cut(s) 131
Bst4CI ACNGT 1 cut(s) 709
Bst6I CTCTTC 2 cut(s) 686, 732
BstACI GRCGYC 1 cut(s) 368
BstAPI GCANNNNNTGC 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 801
BstC8I GCNNGC 4 cut(s) 98, 127, 793, 1198
BstDEI CTNAG 3 cut(s) 260, 1095, 1124
BstF5I GGATG 2 cut(s) 77, 877
BstFNI CGCG 2 cut(s) 272, 1177
BstHHI GCGC 3 cut(s) 117, 400, 1179
BstKTI GATC 9 cut(s) 325, 378, 423, 529, 535, 559, 565, 571, 1010
BstMAI GTCTC 3 cut(s) 54, 500, 995
BstMBI GATC 9 cut(s) 322, 375, 420, 526, 532, 556, 562, 568, 1007
BstMWI GCNNNNNNNGC 3 cut(s) 112, 256, 1193
BstNSI RCATGY 3 cut(s) 485, 795, 848
BstSCI CCNGG 2 cut(s) 542, 885
BstSFI CTRYAG 1 cut(s) 708
BstUI CGCG 2 cut(s) 272, 1177
BstV1I GCAGC 4 cut(s) 124, 382, 451, 677
BstV2I GAAGAC 1 cut(s) 609
BstX2I RGATCY 3 cut(s) 322, 375, 568
BstXI CCANNNNNNTGG 1 cut(s) 978
BstYI RGATCY 3 cut(s) 322, 375, 568
BsuRI GGCC 3 cut(s) 890, 1187, 1196
BtrI CACGTC 1 cut(s) 675
BtsCI GGATG 2 cut(s) 77, 877
BtsIMutI CAGTG 1 cut(s) 45
BveI ACCTGC 1 cut(s) 1046
Cac8I GCNNGC 4 cut(s) 98, 127, 793, 1198
CciI TCATGA 4 cut(s) 165, 492, 529, 856
CfoI GCGC 3 cut(s) 117, 400, 1179
Cfr10I RCCGGY 5 cut(s) 954, 975, 1144, 1162, 1196
Cfr13I GGNCC 5 cut(s) 888, 952, 1058, 1186, 1216
CseI GACGC 1 cut(s) 357
Csp6I GTAC 2 cut(s) 160, 479
CspAI ACCGGT 2 cut(s) 975, 1144
CviQI GTAC 2 cut(s) 160, 479
DdeI CTNAG 3 cut(s) 260, 1095, 1124
DpnI GATC 9 cut(s) 324, 377, 422, 528, 534, 558, 564, 570, 1009
DpnII GATC 9 cut(s) 322, 375, 420, 526, 532, 556, 562, 568, 1007
DriI GACNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 1194
Eam1104I CTCTTC 2 cut(s) 686, 732
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 2 cut(s) 686, 732
Eco130I CCWWGG 2 cut(s) 55, 1061
Eco47I GGWCC 3 cut(s) 952, 1058, 1216
Eco57I CTGAAG 1 cut(s) 645
EcoRI GAATTC 2 cut(s) 758, 962
EcoT14I CCWWGG 2 cut(s) 55, 1061
ErhI CCWWGG 2 cut(s) 55, 1061
Esp3I CGTCTC 2 cut(s) 500, 995
FaqI GGGAC 5 cut(s) 559, 609, 632, 665, 848
FauI CCCGC 4 cut(s) 586, 637, 704, 1193
FbaI TGATCA 2 cut(s) 526, 532
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 5 cut(s) 113, 396, 465, 666, 1175
FokI GGATG 2 cut(s) 84, 884
Fsp4HI GCNGC 5 cut(s) 113, 396, 465, 666, 1175
FspBI CTAG 3 cut(s) 221, 549, 615
GlaI GCGC 3 cut(s) 116, 399, 1178
GluI GCNGC 5 cut(s) 113, 396, 465, 666, 1175
HaeIII GGCC 3 cut(s) 890, 1187, 1196
HgaI GACGC 1 cut(s) 357
HhaI GCGC 3 cut(s) 117, 400, 1179
Hin1I GRCGYC 1 cut(s) 368
Hin6I GCGC 3 cut(s) 115, 398, 1177
HinP1I GCGC 3 cut(s) 115, 398, 1177
HindIII AAGCTT 1 cut(s) 631
HinfI GANTC 6 cut(s) 215, 267, 742, 764, 803, 836
HphI GGTGA 2 cut(s) 622, 736
Hpy166II GTNNAC 2 cut(s) 91, 244
Hpy188I TCNGA 8 cut(s) 309, 373, 448, 779, 1012, 1098, 1142, 1156
Hpy188III TCNNGA 8 cut(s) 166, 424, 493, 530, 560, 566, 857, 986
Hpy8I GTNNAC 2 cut(s) 91, 244
Hpy99I CGWCG 3 cut(s) 373, 514, 1160
HpyAV CCTTC 4 cut(s) 52, 943, 1072, 1201
HpyCH4III ACNGT 1 cut(s) 709
HpyCH4IV ACGT 1 cut(s) 674
HpyCH4V TGCA 3 cut(s) 250, 288, 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 256, 1193
HpyF3I CTNAG 3 cut(s) 260, 1095, 1124
HpySE526I ACGT 1 cut(s) 674
Hsp92I GRCGYC 1 cut(s) 368
HspAI GCGC 3 cut(s) 115, 398, 1177
KroI GCCGGC 1 cut(s) 1196
KroNI GCCGGC 1 cut(s) 1198
Ksp22I TGATCA 2 cut(s) 526, 532
Kzo9I GATC 9 cut(s) 322, 375, 420, 526, 532, 556, 562, 568, 1007
LguI GCTCTTC 1 cut(s) 686
LmnI GCTCC 1 cut(s) 448
Lsp1109I GCAGC 4 cut(s) 124, 382, 451, 677
MaeI CTAG 3 cut(s) 221, 549, 615
MaeII ACGT 1 cut(s) 674
MaeIII GTNAC 3 cut(s) 216, 610, 901
MalI GATC 9 cut(s) 324, 377, 422, 528, 534, 558, 564, 570, 1009
MboI GATC 9 cut(s) 322, 375, 420, 526, 532, 556, 562, 568, 1007
MboII GAAGA 7 cut(s) 194, 430, 485, 614, 673, 749, 939
MflI RGATCY 3 cut(s) 322, 375, 568
MhlI GDGCHC 1 cut(s) 317
MluCI AATT 2 cut(s) 758, 962
MlyI GAGTC 3 cut(s) 224, 773, 830
MmeI TCCRAC 2 cut(s) 351, 1179
MroNI GCCGGC 1 cut(s) 1196
MseI TTAA 3 cut(s) 459, 584, 785
MslI CAYNNNNRTG 1 cut(s) 486
MspR9I CCNGG 2 cut(s) 544, 887
MvnI CGCG 2 cut(s) 272, 1177
MwoI GCNNNNNNNGC 3 cut(s) 112, 256, 1193
NaeI GCCGGC 1 cut(s) 1198
NciI CCSGG 2 cut(s) 544, 887
NdeII GATC 9 cut(s) 322, 375, 420, 526, 532, 556, 562, 568, 1007
NgoMIV GCCGGC 1 cut(s) 1196
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 3 cut(s) 216, 610, 901
NspI RCATGY 3 cut(s) 485, 795, 848
NspV TTCGAA 1 cut(s) 801
PaeI GCATGC 1 cut(s) 795
PagI TCATGA 4 cut(s) 165, 492, 529, 856
PciSI GCTCTTC 1 cut(s) 686
PdiI GCCGGC 1 cut(s) 1198
PfeI GAWTC 3 cut(s) 267, 742, 803
PflMI CCANNNNNTGG 1 cut(s) 675
PfoI TCCNGGA 1 cut(s) 542
PinAI ACCGGT 2 cut(s) 975, 1144
PkrI GCNGC 5 cut(s) 114, 397, 466, 667, 1176
PleI GAGTC 3 cut(s) 223, 772, 830
PpsI GAGTC 3 cut(s) 223, 772, 830
PsiI TTATAA 1 cut(s) 638
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 5 cut(s) 888, 952, 1058, 1186, 1216
PsuI RGATCY 3 cut(s) 322, 375, 568
RsaI GTAC 2 cut(s) 161, 480
RsaNI GTAC 2 cut(s) 160, 479
RseI CAYNNNNRTG 1 cut(s) 486
SapI GCTCTTC 1 cut(s) 686
SaqAI TTAA 3 cut(s) 459, 584, 785
SatI GCNGC 5 cut(s) 113, 396, 465, 666, 1175
Sau3AI GATC 9 cut(s) 322, 375, 420, 526, 532, 556, 562, 568, 1007
Sau96I GGNCC 5 cut(s) 888, 952, 1058, 1186, 1216
SchI GAGTC 3 cut(s) 224, 773, 830
ScrFI CCNGG 2 cut(s) 544, 887
SduI GDGCHC 1 cut(s) 317
SfcI CTRYAG 1 cut(s) 708
SfiI GGCCNNNNNGGCC 1 cut(s) 1193
SfuI TTCGAA 1 cut(s) 801
SinI GGWCC 3 cut(s) 952, 1058, 1216
SmiMI CAYNNNNRTG 1 cut(s) 486
SpeI ACTAGT 1 cut(s) 548
SphI GCATGC 1 cut(s) 795
Sse9I AATT 2 cut(s) 758, 962
SsiI CCGC 8 cut(s) 467, 593, 644, 697, 1129, 1175, 1200, 1219
SspI AATATT 1 cut(s) 81
SspMI CTAG 3 cut(s) 221, 549, 615
StyD4I CCNGG 2 cut(s) 542, 885
StyI CCWWGG 2 cut(s) 55, 1061
TaaI ACNGT 1 cut(s) 709
TaiI ACGT 1 cut(s) 677
TaqI TCGA 5 cut(s) 189, 360, 762, 801, 987
TasI AATT 2 cut(s) 758, 962
TatI WGTACW 2 cut(s) 159, 478
TauI GCSGC 1 cut(s) 1177
TfiI GAWTC 3 cut(s) 267, 742, 803
Tru1I TTAA 3 cut(s) 459, 584, 785
Tru9I TTAA 3 cut(s) 459, 584, 785
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 3 cut(s) 216, 610, 901
TseI GCWGC 4 cut(s) 112, 395, 464, 665
Tsp45I GTSAC 3 cut(s) 216, 610, 901
TspDTI ATGAA 7 cut(s) 182, 242, 397, 509, 615, 845, 1115
TspGWI ACGGA 1 cut(s) 933
TspRI CASTG 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 675
VpaK11BI GGWCC 3 cut(s) 952, 1058, 1216
XapI RAATTY 2 cut(s) 758, 962
XceI RCATGY 3 cut(s) 485, 795, 848
XcmI CCANNNNNNNNNTGG 1 cut(s) 828
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 3 cut(s) 221, 549, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.