pycom111g01470

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0020444
Physical Location & Seq
Forward (+)
939217 .. 940675
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01470.1

Sequence Viewer

Length: 855 bp
ATGCGGCCGCTGTTGTACATGTTTTCATCAAGACAAGGTCAGTTTCAGTTGGGGGAAAAAAGTGTTCACTGGTTTATTAATCTTTCTTCGGGTTTCTTTTCATTGAAGGTGGTAAGGGTTTTAAGAGCCAATCCAGTATTCGTTACAGCACTTGTTGCAATTACTCTGGCGTGCTTGGTTCATGCAGCGTTCGGTCTATTTATAATCTTGTTCTTCGATGCTTTGAGCTGTCACAGTGCACTTTGCAGTTTTTTGACAGCTTCATTTCGCAGTCATGCACGAAGACATGAATTATTTGACTGTAAAAAAGAAGACAATGACCAGTCCTGCCAACTTCCATCCAAAAGTGATGGTATATCAAACCAGAATATTCATTCTGAAGATTGCTGCTCCAATAGTCCAAACTCTTCGAAAAGTTTTGGTGAAACACAGTTAGAGTTATTCCATCATCAGCATGGGCTGTTTATTTTGCATCTTGCTGCAGCACTCATGTTTGTCCCGTCACTTGTCGCCTGGTTTCAGAGAATAGGGATGGGTCATAGCTTCCCATGGCTCTTGGATTCATTTCTTTGCACAGGTGTGATCCTTCATGGAATCTTCACCGCAAAGCCCGAGTCCAGTTCCTTCTTGGTATCTTTCCCTGGCTTCCGAAACTGTGAAGTGAGGCTGAATTTTCTGTACCTGCTGGCTGGATACTATTCCTATATCTCTTCCCTGGCCTTGGCTCCATACAGAGCATTTTATGGTATGGCTGCTATAGGCTTTACTTGCTGTGCTTTGACGATCTTACAAAATCGGAACAGAGAAAAGGGTGAGCCCCATTTTGTTAGCCGAAAGCACTCCCACAGGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PGAP1

Protein Analysis

285

Amino Acids

32.02

Weight (kDa)

8.9

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020444)

Species Orthologous Gene IDs
pyrus_communis pycom111g01470 pycom17g01520
rosa_laevigata RLG00000022258
rosa_multiflora Rmu_sc0043101.1_g000001
rosa_samantha Rh2CG631200 Rh2DG681800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 690
AciI CCGC 3 cut(s) 4, 8, 603
AclWI GGATC 1 cut(s) 577
AcoI YGGCCR 1 cut(s) 5
AcsI RAATTY 1 cut(s) 670
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 2 cut(s) 17, 680
AfiI CCNNNNNNNGG 1 cut(s) 721
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 3 cut(s) 512, 640, 714
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AleI CACNNNNGTG 1 cut(s) 578
AluBI AGCT 3 cut(s) 228, 260, 543
AluI AGCT 3 cut(s) 228, 260, 543
Alw21I GWGCWC 1 cut(s) 241
Alw44I GTGCAC 1 cut(s) 237
AlwI GGATC 1 cut(s) 577
Ama87I CYCGRG 1 cut(s) 611
AoxI GGCC 2 cut(s) 5, 717
ApaLI GTGCAC 1 cut(s) 237
ApeKI GCWGC 5 cut(s) 185, 387, 479, 482, 752
ApoI RAATTY 1 cut(s) 670
ArsI GACNNNNNNTTYG 2 cut(s) 599, 631
AseI ATTAAT 1 cut(s) 78
AsuHPI GGTGA 3 cut(s) 434, 592, 824
AsuII TTCGAA 1 cut(s) 410
AvaI CYCGRG 1 cut(s) 611
BaeGI GKGCMC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 819
BbsI GAAGAC 2 cut(s) 289, 318
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 5 cut(s) 197, 374, 466, 494, 739
BccI CCATC 4 cut(s) 344, 346, 453, 526
BciT130I CCWGG 3 cut(s) 514, 642, 716
BciVI GTATCC 1 cut(s) 686
BfmI CTRYAG 2 cut(s) 480, 756
BfuAI ACCTGC 1 cut(s) 690
BfuI GTATCC 1 cut(s) 686
BisI GCNGC 7 cut(s) 5, 8, 186, 388, 480, 483, 753
BlsI GCNGC 7 cut(s) 6, 9, 187, 389, 481, 484, 754
Bme1390I CCNGG 3 cut(s) 514, 642, 716
BmeT110I CYCGRG 1 cut(s) 611
BmiI GGNNCC 1 cut(s) 726
BmrFI CCNGG 3 cut(s) 514, 642, 716
BmsI GCATC 2 cut(s) 208, 481
BpiI GAAGAC 2 cut(s) 289, 318
Bpu14I TTCGAA 1 cut(s) 410
BsaJI CCNNGG 4 cut(s) 548, 640, 714, 720
Bsc4I CCNNNNNNNGG 1 cut(s) 721
Bse1I ACTGG 4 cut(s) 74, 134, 322, 618
BseBI CCWGG 3 cut(s) 514, 642, 716
BseDI CCNNGG 4 cut(s) 548, 640, 714, 720
BseGI GGATG 2 cut(s) 338, 537
BseLI CCNNNNNNNGG 1 cut(s) 721
BseNI ACTGG 4 cut(s) 74, 134, 322, 618
BseSI GKGCMC 1 cut(s) 241
BseX3I CGGCCG 1 cut(s) 5
BseXI GCAGC 5 cut(s) 197, 374, 466, 494, 739
Bsh1285I CGRYCG 1 cut(s) 8
BshFI GGCC 2 cut(s) 7, 719
BsiEI CGRYCG 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 241
BsiHKCI CYCGRG 1 cut(s) 611
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 1 cut(s) 721
BsmFI GGGAC 1 cut(s) 482
BsnI GGCC 2 cut(s) 7, 719
BsoBI CYCGRG 1 cut(s) 611
Bsp119I TTCGAA 1 cut(s) 410
Bsp1286I GDGCHC 2 cut(s) 241, 819
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 2 cut(s) 582, 783
Bsp19I CCATGG 1 cut(s) 548
BspACI CCGC 3 cut(s) 4, 8, 603
BspANI GGCC 2 cut(s) 7, 719
BspLI GGNNCC 1 cut(s) 726
BspMAI CTGCAG 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 690
BspPI GGATC 1 cut(s) 577
BspT104I TTCGAA 1 cut(s) 410
BsrGI TGTACA 1 cut(s) 15
BsrI ACTGG 4 cut(s) 74, 134, 322, 618
BssECI CCNNGG 4 cut(s) 548, 640, 714, 720
BssMI GATC 2 cut(s) 582, 783
BssT1I CCWWGG 2 cut(s) 548, 720
Bst2UI CCWGG 3 cut(s) 514, 642, 716
Bst4CI ACNGT 4 cut(s) 236, 302, 432, 656
Bst6I CTCTTC 2 cut(s) 412, 715
BstAPI GCANNNNNTGC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 410
BstC8I GCNNGC 2 cut(s) 172, 687
BstDSI CCRYGG 1 cut(s) 548
BstF5I GGATG 2 cut(s) 338, 537
BstKTI GATC 2 cut(s) 585, 786
BstMBI GATC 2 cut(s) 582, 783
BstMCI CGRYCG 1 cut(s) 8
BstMWI GCNNNNNNNGC 2 cut(s) 155, 768
BstNI CCWGG 3 cut(s) 514, 642, 716
BstNSI RCATGY 1 cut(s) 22
BstSCI CCNGG 3 cut(s) 512, 640, 714
BstSFI CTRYAG 2 cut(s) 480, 756
BstSLI GKGCMC 1 cut(s) 241
BstV1I GCAGC 5 cut(s) 197, 374, 466, 494, 739
BstV2I GAAGAC 2 cut(s) 289, 318
BstZI CGGCCG 1 cut(s) 5
BsuI GTATCC 1 cut(s) 686
BsuRI GGCC 2 cut(s) 7, 719
BtgI CCRYGG 1 cut(s) 548
BtsCI GGATG 2 cut(s) 338, 537
BtsIMutI CAGTG 2 cut(s) 67, 241
BveI ACCTGC 1 cut(s) 690
Cac8I GCNNGC 2 cut(s) 172, 687
CciNI GCGGCCGC 1 cut(s) 5
Csp6I GTAC 2 cut(s) 16, 679
CviAII CATG 8 cut(s) 19, 182, 275, 287, 455, 490, 549, 590
CviQI GTAC 2 cut(s) 16, 679
DpnI GATC 2 cut(s) 584, 785
DpnII GATC 2 cut(s) 582, 783
EaeI YGGCCR 1 cut(s) 5
EagI CGGCCG 1 cut(s) 5
Eam1104I CTCTTC 2 cut(s) 412, 715
EarI CTCTTC 2 cut(s) 412, 715
EclXI CGGCCG 1 cut(s) 5
Eco130I CCWWGG 2 cut(s) 548, 720
Eco24I GRGCYC 1 cut(s) 819
Eco52I CGGCCG 1 cut(s) 5
Eco57I CTGAAG 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 611
EcoRII CCWGG 3 cut(s) 512, 640, 714
EcoT14I CCWWGG 2 cut(s) 548, 720
EcoT38I GRGCYC 1 cut(s) 819
ErhI CCWWGG 2 cut(s) 548, 720
FaeI CATG 8 cut(s) 22, 185, 278, 290, 458, 493, 552, 593
FaqI GGGAC 1 cut(s) 482
FatI CATG 8 cut(s) 18, 181, 274, 286, 454, 489, 548, 589
Fnu4HI GCNGC 7 cut(s) 5, 8, 186, 388, 480, 483, 753
FokI GGATG 2 cut(s) 325, 544
FriOI GRGCYC 1 cut(s) 819
Fsp4HI GCNGC 7 cut(s) 5, 8, 186, 388, 480, 483, 753
GluI GCNGC 7 cut(s) 5, 8, 186, 388, 480, 483, 753
HaeIII GGCC 2 cut(s) 7, 719
Hin1II CATG 8 cut(s) 22, 185, 278, 290, 458, 493, 552, 593
HinfI GANTC 3 cut(s) 560, 594, 614
HphI GGTGA 3 cut(s) 434, 592, 824
Hpy166II GTNNAC 2 cut(s) 67, 239
Hpy188I TCNGA 4 cut(s) 379, 522, 650, 798
Hpy188III TCNNGA 1 cut(s) 30
Hpy8I GTNNAC 2 cut(s) 67, 239
HpyAV CCTTC 3 cut(s) 100, 596, 634
HpyCH4III ACNGT 4 cut(s) 236, 302, 432, 656
HpyCH4V TGCA 8 cut(s) 158, 185, 239, 246, 278, 472, 482, 573
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 768
Hsp92II CATG 8 cut(s) 22, 185, 278, 290, 458, 493, 552, 593
Kzo9I GATC 2 cut(s) 582, 783
LmnI GCTCC 2 cut(s) 395, 730
Lsp1109I GCAGC 5 cut(s) 197, 374, 466, 494, 739
LweI GCATC 2 cut(s) 208, 481
MaeIII GTNAC 3 cut(s) 142, 230, 501
MalI GATC 2 cut(s) 584, 785
MboI GATC 2 cut(s) 582, 783
MboII GAAGA 8 cut(s) 78, 205, 294, 323, 392, 399, 589, 702
MhlI GDGCHC 2 cut(s) 241, 819
MluCI AATT 3 cut(s) 159, 290, 670
MlyI GAGTC 1 cut(s) 623
MnlI CCTC 1 cut(s) 657
MseI TTAA 2 cut(s) 78, 122
MslI CAYNNNNRTG 2 cut(s) 453, 578
MspA1I CMGCKG 1 cut(s) 10
MspR9I CCNGG 3 cut(s) 514, 642, 716
MvaI CCWGG 3 cut(s) 514, 642, 716
MwoI GCNNNNNNNGC 2 cut(s) 155, 768
NcoI CCATGG 1 cut(s) 548
NdeII GATC 2 cut(s) 582, 783
NlaIII CATG 8 cut(s) 22, 185, 278, 290, 458, 493, 552, 593
NlaIV GGNNCC 1 cut(s) 726
NmuCI GTSAC 2 cut(s) 230, 501
NotI GCGGCCGC 1 cut(s) 5
NspI RCATGY 1 cut(s) 22
NspV TTCGAA 1 cut(s) 410
OliI CACNNNNGTG 1 cut(s) 578
PciI ACATGT 1 cut(s) 18
PfeI GAWTC 2 cut(s) 560, 594
PflFI GACNNNGTC 1 cut(s) 36
PkrI GCNGC 7 cut(s) 6, 9, 187, 389, 481, 484, 754
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
PscI ACATGT 1 cut(s) 18
PshBI ATTAAT 1 cut(s) 78
PsiI TTATAA 1 cut(s) 203
Psp6I CCWGG 3 cut(s) 512, 640, 714
PspGI CCWGG 3 cut(s) 512, 640, 714
PspN4I GGNNCC 1 cut(s) 726
PstI CTGCAG 1 cut(s) 484
PsyI GACNNNGTC 1 cut(s) 36
RsaI GTAC 2 cut(s) 17, 680
RsaNI GTAC 2 cut(s) 16, 679
RseI CAYNNNNRTG 2 cut(s) 453, 578
SaqAI TTAA 2 cut(s) 78, 122
SatI GCNGC 7 cut(s) 5, 8, 186, 388, 480, 483, 753
Sau3AI GATC 2 cut(s) 582, 783
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 3 cut(s) 514, 642, 716
SduI GDGCHC 2 cut(s) 241, 819
SetI ASST 7 cut(s) 40, 111, 230, 262, 545, 580, 684
SfaNI GCATC 2 cut(s) 208, 481
SfcI CTRYAG 2 cut(s) 480, 756
SfuI TTCGAA 1 cut(s) 410
SmiMI CAYNNNNRTG 2 cut(s) 453, 578
Sse9I AATT 3 cut(s) 159, 290, 670
SsiI CCGC 3 cut(s) 4, 8, 603
SspI AATATT 1 cut(s) 370
StyD4I CCNGG 3 cut(s) 512, 640, 714
StyI CCWWGG 2 cut(s) 548, 720
TaaI ACNGT 4 cut(s) 236, 302, 432, 656
TaqI TCGA 2 cut(s) 216, 410
TaqII GACCGA 1 cut(s) 182
TasI AATT 3 cut(s) 159, 290, 670
TatI WGTACW 1 cut(s) 15
TauI GCSGC 2 cut(s) 7, 10
TfiI GAWTC 2 cut(s) 560, 594
Tru1I TTAA 2 cut(s) 78, 122
Tru9I TTAA 2 cut(s) 78, 122
TscAI CASTG 2 cut(s) 74, 241
TseFI GTSAC 2 cut(s) 230, 501
TseI GCWGC 5 cut(s) 185, 387, 479, 482, 752
Tsp45I GTSAC 2 cut(s) 230, 501
TspDTI ATGAA 8 cut(s) 15, 90, 170, 252, 303, 362, 552, 578
TspRI CASTG 2 cut(s) 74, 241
Tth111I GACNNNGTC 1 cut(s) 36
VneI GTGCAC 1 cut(s) 237
VspI ATTAAT 1 cut(s) 78
XapI RAATTY 1 cut(s) 670
XceI RCATGY 1 cut(s) 22
XcmI CCANNNNNNNNNTGG 2 cut(s) 452, 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.