RLG00000022258

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020444
Physical Location & Seq
Forward (+)
82030539 .. 82035619
5081 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022258

Sequence Viewer

Length: 1233 bp
ATGCTTCCATCACAATCCACAATTCAAAGCCACCTTTGCAACGAGCTCTTTTTCTTCAAGAAAGTGGTGGCATCTTCTGGCCCAGGTGCTCAACAACCGCAGGACTGTCTTGTTGCGGGCGTTAGTTGTGACGGTTTATTGGCTATTTGTAGGCCGACTGATACATCGTGGAGCCTTATTAAAATAGACGGGCTCAGGTCTAGAGATATAGAAATAATTGATGAAAAATTGTACACTATAACTCAAATCCACACCTCCGAGTTTATAGTGGTATATAAGACCCGCCCTACAGATGCCGGAGGCATTAGAATAGAAATGGTTTTGATTCCAGTCAAGCGTCAAAGCCACAGAAAAGGGCTCTTCCGAGTGTTCAAGCTGGAGTCTAGCCTCACTCATAGACTAACTCTCGGTGCTCGTTGGGCGGAGGTAGATCCACATTGTTCATACAGCAGTGCGATGACTGTTTCTGTAACTGCAGTTGCCAGTAGGTTTTTGCTTGTGTATACTTCACAGAAAGACGGAAATGGTGGGCCTGACAAAATTACATTCAAACGTGATGGTATATCAGACCAGAATTTTTCTTCTGAAGATACGTCCTCCAATAGCCCAGACTCTTCAAAAAGTTTTGGTGAAACTCAATTAGAGATATTCCATCATCCGCATGGGTTGTTTATTCTGCATCTTGTTGCAACACTCATGTTTGGCCCATCATTTGTGACTTGGTTGGAGAGAATTGGGACGGATCGTAGCTTTCCATGGCTCTTGGATTCAGCTCTTTCCACAGGTGTGATCCTTCATGGTATCTTCACTTCAAATCCTCAGTTCAATTCCTTCCTGGTTTCTTTGCCACCAATCCGAAACCTAGAAGTGAGGATGCATTTACTGTACCTGTTTGCTGGACACTATTCCTATCTCTCTTCCCTGGCCTTGGCTCCTTATAGAGAATTTTATGTTATGTCTGCTGTAGGCTATACATCTATTGGTCTGACCCTCTTACAAAGATGGAACAAGGAGAAAGGAGATGCCCATTTTGTTAACCGAAGGCACTCTCACAGGCACCGACTCCCCAGAAATACTCAGATTTTACTCAGTGGCCAAATCTCCAGTAAGGTCCCTTGTGCTAATATAGGTTACGAGAACATGTTAGTCCTTCTGAGGGTGTGGGACTTGTTTGCGGACTTGCTTCTATTAACCACAGTTCTGCAATCAGAATTCTCACGGACAAGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PGAP1

Protein Analysis

411

Amino Acids

46.07

Weight (kDa)

8.95

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 9 - 92 2.4e-06 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020444)

Species Orthologous Gene IDs
pyrus_communis pycom111g01470 pycom17g01520
rosa_laevigata RLG00000022258
rosa_multiflora Rmu_sc0043101.1_g000001
rosa_samantha Rh2CG631200 Rh2DG681800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1056
AccI GTMKAC 1 cut(s) 503
AciI CCGC 6 cut(s) 98, 116, 283, 422, 659, 1175
AclWI GGATC 3 cut(s) 425, 750, 784
AcoI YGGCCR 1 cut(s) 1093
AcsI RAATTY 3 cut(s) 574, 944, 1211
AcuI CTGAAG 1 cut(s) 606
AfaI GTAC 2 cut(s) 233, 887
AfiI CCNNNNNNNGG 2 cut(s) 928, 1156
AflIII ACRYGT 1 cut(s) 1140
AgsI TTSAA 7 cut(s) 26, 58, 373, 550, 618, 813, 826
AjnI CCWGG 3 cut(s) 82, 834, 921
AleI CACNNNNGTG 1 cut(s) 785
AluBI AGCT 4 cut(s) 46, 376, 750, 773
AluI AGCT 4 cut(s) 46, 376, 750, 773
Alw21I GWGCWC 3 cut(s) 48, 91, 415
AlwI GGATC 3 cut(s) 425, 750, 784
AoxI GGCC 6 cut(s) 79, 152, 530, 703, 924, 1093
ApoI RAATTY 3 cut(s) 574, 944, 1211
AspS9I GGNCC 4 cut(s) 80, 530, 704, 1111
AsuHPI GGTGA 1 cut(s) 641
AvaII GGWCC 1 cut(s) 1111
BalI TGGCCA 1 cut(s) 1095
BanI GGYRCC 1 cut(s) 1056
BanII GRGCYC 3 cut(s) 48, 195, 360
BarI GAAGNNNNNNTAC 2 cut(s) 793, 825
Bbv12I GWGCWC 3 cut(s) 48, 91, 415
BccI CCATC 5 cut(s) 16, 551, 660, 715, 996
BciT130I CCWGG 3 cut(s) 84, 836, 923
BfaI CTAG 3 cut(s) 201, 384, 863
BfmI CTRYAG 3 cut(s) 288, 474, 963
Bme1390I CCNGG 3 cut(s) 84, 836, 923
Bme18I GGWCC 1 cut(s) 1111
BmgT120I GGNCC 4 cut(s) 80, 530, 704, 1111
BmiI GGNNCC 4 cut(s) 173, 933, 1058, 1113
BmrFI CCNGG 3 cut(s) 84, 836, 923
BmsI GCATC 5 cut(s) 80, 283, 688, 864, 1012
BpmI CTGGAG 2 cut(s) 398, 1087
Bpu10I CCTNAGC 1 cut(s) 194
BsaJI CCNNGG 4 cut(s) 82, 755, 921, 927
Bsc4I CCNNNNNNNGG 2 cut(s) 928, 1156
Bse1I ACTGG 3 cut(s) 329, 483, 1104
BseBI CCWGG 3 cut(s) 84, 836, 923
BseDI CCNNGG 4 cut(s) 82, 755, 921, 927
BseGI GGATG 2 cut(s) 655, 879
BseLI CCNNNNNNNGG 2 cut(s) 928, 1156
BseMII CTCAG 5 cut(s) 208, 833, 1091, 1102, 1145
BseNI ACTGG 3 cut(s) 329, 483, 1104
BshFI GGCC 6 cut(s) 81, 154, 532, 705, 926, 1095
BshNI GGYRCC 1 cut(s) 1056
BsiHKAI GWGCWC 3 cut(s) 48, 91, 415
BsiSI CCGG 1 cut(s) 297
BslFI GGGAC 3 cut(s) 751, 1097, 1178
BslI CCNNNNNNNGG 2 cut(s) 928, 1156
BsmFI GGGAC 3 cut(s) 751, 1097, 1178
BsnI GGCC 6 cut(s) 81, 154, 532, 705, 926, 1095
Bsp1286I GDGCHC 5 cut(s) 48, 91, 195, 360, 415
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 3 cut(s) 430, 742, 789
Bsp19I CCATGG 1 cut(s) 755
BspACI CCGC 6 cut(s) 98, 116, 283, 422, 659, 1175
BspANI GGCC 6 cut(s) 81, 154, 532, 705, 926, 1095
BspCNI CTCAG 5 cut(s) 207, 832, 1090, 1101, 1146
BspLI GGNNCC 4 cut(s) 173, 933, 1058, 1113
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 3 cut(s) 425, 750, 784
BspQI GCTCTTC 1 cut(s) 365
BspT107I GGYRCC 1 cut(s) 1056
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 3 cut(s) 329, 483, 1104
BssECI CCNNGG 4 cut(s) 82, 755, 921, 927
BssMI GATC 3 cut(s) 430, 742, 789
BssNAI GTATAC 1 cut(s) 504
BssT1I CCWWGG 2 cut(s) 755, 927
Bst1107I GTATAC 1 cut(s) 504
Bst2UI CCWGG 3 cut(s) 84, 836, 923
Bst4CI ACNGT 5 cut(s) 107, 134, 463, 885, 1198
Bst6I CTCTTC 3 cut(s) 365, 619, 922
BstAUI TGTACA 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 5 cut(s) 194, 819, 1077, 1088, 1154
BstDSI CCRYGG 1 cut(s) 755
BstF5I GGATG 2 cut(s) 655, 879
BstKTI GATC 3 cut(s) 433, 745, 792
BstMBI GATC 3 cut(s) 430, 742, 789
BstMWI GCNNNNNNNGC 2 cut(s) 36, 419
BstNI CCWGG 3 cut(s) 84, 836, 923
BstNSI RCATGY 1 cut(s) 1144
BstSCI CCNGG 3 cut(s) 82, 834, 921
BstSFI CTRYAG 3 cut(s) 288, 474, 963
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BstZ17I GTATAC 1 cut(s) 504
BsuRI GGCC 6 cut(s) 81, 154, 532, 705, 926, 1095
BtgI CCRYGG 1 cut(s) 755
BtgZI GCGATG 1 cut(s) 470
BtsCI GGATG 2 cut(s) 655, 879
BtsI GCAGTG 1 cut(s) 457
BtsIMutI CAGTG 2 cut(s) 457, 1096
Cac8I GCNNGC 1 cut(s) 118
Cfr13I GGNCC 4 cut(s) 80, 530, 704, 1111
CseI GACGC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 232, 886
CviAII CATG 5 cut(s) 662, 697, 756, 797, 1141
CviQI GTAC 2 cut(s) 232, 886
DdeI CTNAG 5 cut(s) 194, 819, 1077, 1088, 1154
DpnI GATC 3 cut(s) 432, 744, 791
DpnII GATC 3 cut(s) 430, 742, 789
EaeI YGGCCR 1 cut(s) 1093
Eam1104I CTCTTC 3 cut(s) 365, 619, 922
EarI CTCTTC 3 cut(s) 365, 619, 922
EciI GGCGGA 1 cut(s) 437
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 755, 927
Eco24I GRGCYC 3 cut(s) 48, 195, 360
Eco47I GGWCC 1 cut(s) 1111
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 606
EcoICRI GAGCTC 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 1111
EcoRI GAATTC 1 cut(s) 1211
EcoRII CCWGG 3 cut(s) 82, 834, 921
EcoT14I CCWWGG 2 cut(s) 755, 927
EcoT22I ATGCAT 1 cut(s) 879
EcoT38I GRGCYC 3 cut(s) 48, 195, 360
ErhI CCWWGG 2 cut(s) 755, 927
FaeI CATG 5 cut(s) 665, 700, 759, 800, 1144
FaqI GGGAC 3 cut(s) 751, 1097, 1178
FatI CATG 5 cut(s) 661, 696, 755, 796, 1140
FauI CCCGC 2 cut(s) 109, 290
FblI GTMKAC 1 cut(s) 503
FokI GGATG 2 cut(s) 642, 886
FriOI GRGCYC 3 cut(s) 48, 195, 360
FspBI CTAG 3 cut(s) 201, 384, 863
GsuI CTGGAG 2 cut(s) 398, 1087
HaeIII GGCC 6 cut(s) 81, 154, 532, 705, 926, 1095
HapII CCGG 1 cut(s) 297
HgaI GACGC 1 cut(s) 326
Hin1II CATG 5 cut(s) 665, 700, 759, 800, 1144
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HinfI GANTC 5 cut(s) 325, 380, 611, 767, 1062
HpaI GTTAAC 1 cut(s) 1036
HpaII CCGG 1 cut(s) 297
HphI GGTGA 1 cut(s) 641
Hpy166II GTNNAC 3 cut(s) 234, 504, 1036
Hpy188I TCNGA 9 cut(s) 259, 365, 568, 586, 857, 987, 1080, 1155, 1210
Hpy188III TCNNGA 2 cut(s) 58, 201
Hpy8I GTNNAC 3 cut(s) 234, 504, 1036
HpyAV CCTTC 4 cut(s) 803, 841, 1035, 1160
HpyCH4III ACNGT 5 cut(s) 107, 134, 463, 885, 1198
HpyCH4IV ACGT 2 cut(s) 553, 593
HpyCH4V TGCA 6 cut(s) 39, 476, 679, 689, 877, 1204
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 419
HpyF3I CTNAG 5 cut(s) 194, 819, 1077, 1088, 1154
HpySE526I ACGT 2 cut(s) 553, 593
Hsp92II CATG 5 cut(s) 665, 700, 759, 800, 1144
KspAI GTTAAC 1 cut(s) 1036
Kzo9I GATC 3 cut(s) 430, 742, 789
LguI GCTCTTC 1 cut(s) 365
LmnI GCTCC 2 cut(s) 171, 937
LweI GCATC 5 cut(s) 80, 283, 688, 864, 1012
MaeI CTAG 3 cut(s) 201, 384, 863
MaeII ACGT 2 cut(s) 553, 593
MaeIII GTNAC 4 cut(s) 128, 469, 715, 1130
MalI GATC 3 cut(s) 432, 744, 791
MboI GATC 3 cut(s) 430, 742, 789
MboII GAAGA 8 cut(s) 46, 66, 352, 573, 599, 606, 796, 909
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 5 cut(s) 48, 91, 195, 360, 415
MlsI TGGCCA 1 cut(s) 1095
MluNI TGGCCA 1 cut(s) 1095
MlyI GAGTC 3 cut(s) 389, 605, 1056
MmeI TCCRAC 1 cut(s) 705
MnlI CCTC 9 cut(s) 265, 293, 398, 418, 607, 828, 864, 1001, 1149
Mox20I TGGCCA 1 cut(s) 1095
Mph1103I ATGCAT 1 cut(s) 879
MscI TGGCCA 1 cut(s) 1095
MseI TTAA 3 cut(s) 180, 1035, 1190
MslI CAYNNNNRTG 2 cut(s) 660, 785
Msp20I TGGCCA 1 cut(s) 1095
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 3 cut(s) 84, 836, 923
MvaI CCWGG 3 cut(s) 84, 836, 923
MwoI GCNNNNNNNGC 2 cut(s) 36, 419
NcoI CCATGG 1 cut(s) 755
NdeII GATC 3 cut(s) 430, 742, 789
NlaIII CATG 5 cut(s) 665, 700, 759, 800, 1144
NlaIV GGNNCC 4 cut(s) 173, 933, 1058, 1113
NmuCI GTSAC 2 cut(s) 128, 715
NsiI ATGCAT 1 cut(s) 879
NspI RCATGY 1 cut(s) 1144
OliI CACNNNNGTG 1 cut(s) 785
PciI ACATGT 1 cut(s) 1140
PciSI GCTCTTC 1 cut(s) 365
PfeI GAWTC 2 cut(s) 325, 767
PleI GAGTC 3 cut(s) 388, 605, 1056
PpsI GAGTC 3 cut(s) 388, 605, 1056
PpuMI RGGWCCY 1 cut(s) 1111
PscI ACATGT 1 cut(s) 1140
Psp124BI GAGCTC 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 1111
Psp6I CCWGG 3 cut(s) 82, 834, 921
PspGI CCWGG 3 cut(s) 82, 834, 921
PspN4I GGNNCC 4 cut(s) 173, 933, 1058, 1113
PspPI GGNCC 4 cut(s) 80, 530, 704, 1111
PspPPI RGGWCCY 1 cut(s) 1111
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 1 cut(s) 430
RsaI GTAC 2 cut(s) 233, 887
RsaNI GTAC 2 cut(s) 232, 886
RseI CAYNNNNRTG 2 cut(s) 660, 785
SacI GAGCTC 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 365
SaqAI TTAA 3 cut(s) 180, 1035, 1190
Sau3AI GATC 3 cut(s) 430, 742, 789
Sau96I GGNCC 4 cut(s) 80, 530, 704, 1111
SchI GAGTC 3 cut(s) 389, 605, 1056
ScrFI CCNGG 3 cut(s) 84, 836, 923
SduI GDGCHC 5 cut(s) 48, 91, 195, 360, 415
SfaNI GCATC 5 cut(s) 80, 283, 688, 864, 1012
SfcI CTRYAG 3 cut(s) 288, 474, 963
SinI GGWCC 1 cut(s) 1111
SmiMI CAYNNNNRTG 2 cut(s) 660, 785
SsiI CCGC 6 cut(s) 98, 116, 283, 422, 659, 1175
SspMI CTAG 3 cut(s) 201, 384, 863
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 3 cut(s) 82, 834, 921
StyI CCWWGG 2 cut(s) 755, 927
TaaI ACNGT 5 cut(s) 107, 134, 463, 885, 1198
TaiI ACGT 2 cut(s) 556, 596
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 2 cut(s) 325, 767
Tru1I TTAA 3 cut(s) 180, 1035, 1190
Tru9I TTAA 3 cut(s) 180, 1035, 1190
TscAI CASTG 2 cut(s) 457, 1096
TseFI GTSAC 2 cut(s) 128, 715
Tsp45I GTSAC 2 cut(s) 128, 715
TspDTI ATGAA 3 cut(s) 237, 432, 785
TspGWI ACGGA 2 cut(s) 534, 755
TspRI CASTG 2 cut(s) 457, 1096
VpaK11BI GGWCC 1 cut(s) 1111
XapI RAATTY 3 cut(s) 574, 944, 1211
XbaI TCTAGA 1 cut(s) 200
XceI RCATGY 1 cut(s) 1144
XcmI CCANNNNNNNNNTGG 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 503
XspI CTAG 3 cut(s) 201, 384, 863
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.