pycom11g04820

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0016555
Physical Location & Seq
Reverse (-)
3698562 .. 3725678
27117 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1926 bp
ATGGAGGCCTCCAAACTTACCCTTCTGCTTGGCCTTCTTGCCTTCCTCACTTTCTTAGCTTCCTTCTCTGCTGCAGAAGATCACTTCCATGAATTCGTTGTTGAAGCGAGGCCGGTGAGGAGGCTGTGCAGAACCCACAGCAGAATCACAGTCAATGGCCAGTTCCCAGGACCAACTTTGGAAGTCAGAAATGGAGATTCCGTTGCCGTCAAAGTTACCAACATAGCGAAATACAACATCACCATCCACTGGCATGGACTAAAGCAGCAGAACAATCCATGGGCAGATGGTCCGGAATACGTCACACAGTGCCCTATCCAACCAGGAGCTACCTACACATACAGGTTCACAATCACAGACCAAGAGGGCACTTTGTGGTGGCATGCTCATAGCAGATGGCTCAGAGCCACTGTCTACGGAGCCCTCATAATCTACCCTAGATTGGGTTCTCCCTACCCCTTCCCGATGCCAAAACGTGAAGTTCCCCTTCTTCTTGGGGAATGGTTTGATCGAAACCCCCAAGATGTCTTGAGGCTTGCAGCTTTTACAGGAGGAGCACCCAATGTCTCTAATGCATACACCATTAACGGCCAGCCTGGTGATCTTTACAGATGTTCCAAAAAAGAAACCATTCGAATTCCAGTCGTATCGGGCGAGTCACTTCTCCTGAGAGTCATCAACTCCGCACTCAATCAAGAACTCTTCTTTACCGTTGCCAACCACCAACTGACTGTTGTGGGTGCTGACGCATCTTACACAAAACCTCTCACAACCAGAGTTATCATGATAGGCCCTGGCCAAACAACCGATGTTCTGCTTACTGCTGATCAGGGCCCTGCTCGGTACTACATGGCTGCTCGTGCCTATCAGACTGGCCTGAATGTGCTCTTCGACAATACCACCACCACTGCTATCCTTGAGTACAAGAATGCTGCTTCTTCAAATCAAGGTTTAGCACCAGTATTTCCTACATTGCCAGCTTACAACGACACAGCAACTGCTACTGCATACACTGCTCAGATAAAGAGCCTATCCCAGGTCAAAGTCCCCACACAGATTGACGAGAACCTGTTTTTCACTGTGGGTTTAGGACTTTATAACTGCACAGTTCCCAACAGCCCCCGGTGTCAAGGACCAAACAACACCCGCTTTGCAGCTAGCATGAACAATGTTTCCTTTGTATTACCAAGAACCAACTCCTTAATGCAAGCTGCCTACTATGGTGTCCCTGGTGTCTTCACCACAGACTTTCCACCTGTTCCTCTTGTGCAATTCAATTATACCGGAAATGTACCCCGGGGGCTATGGTCACCTCGTCGTGGAACTAAGCTCTACAAGTTGAAGTATGGTGCTAATGTACAAATAGTGTTTCAGGACACAAGTATTGTGACTACTGACGACCATCCTATGCATCTTCATGGGTACCAATTCTACGTGATTGGATCAGGATTTGGAAACTACAACCCCAGCACAGATCCAGCGAGATTCAACCTCATTGACCCGCCACAGAGGAATACCATCGGAACAGGTCCAGGAGGATGGGTAGCTATACGATTTAAGGCTGATAATCCAGGTGTCTGGCTGATGCACTGTCACTTAGATTCACATCTCTTCTTGGGTTTGGCTATGGCTTTTCAAGTAGAGAACGGAAACGGACCATTACAGTCTGTAATTCCTCCTCCTCCAGATCTACCCCGATCAGAGGACATCGGGCAAGATGAGGATGTTGCAGACACCTTTGTGCTTCAAGAAGCCATAGCTGAGGCAGAGAGGCAAGGTGTTGGAATAGGTGAGGATGTCTCCGAACTATTTGGGGACTCTGAGAGTGAGGCAGAGGTTGTAGCGGAGCCTTGGGCAACGCATTTGACTCAGTCAACAATAATAGAAACTTCAGAGTTTGAGCCCAAAGAAAATCAAGTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

642

Amino Acids

70.72

Weight (kDa)

5.7

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 36 - 147 3.3e-44 Multicopper oxidase
Cu-oxidase PF00394 160 - 311 4.7e-41 Multicopper oxidase
Cu-oxidase_2 PF07731 417 - 550 4.7e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30210
fragaria_vesca FvH4_3g39750
malus_domestica MD03G1056400.v1.1 MD11G1057600.v1.1 MD11G1058200.v1.1
pyrus_communis pycom03g04470 pycom11g04820
rosa_chinensis RchiOBHm_Chr5g0071631
rosa_laevigata RLG00000036231 RLG00000036234
rosa_multiflora Rmu_sc0005745.1_g000031
rosa_rugosa Rorug05G0413700
rosa_samantha Rh5CG513000
rosa_wichuraiana Rw5G043640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1098
Acc65I GGTACC 1 cut(s) 1422
AccB1I GGYRCC 1 cut(s) 1422
AccB7I CCANNNNNTGG 1 cut(s) 249
AccI GTMKAC 1 cut(s) 414
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 4 cut(s) 684, 1147, 1502, 1844
AclWI GGATC 2 cut(s) 1450, 1469
AcoI YGGCCR 3 cut(s) 157, 589, 796
AcsI RAATTY 2 cut(s) 92, 636
AcuI CTGAAG 1 cut(s) 1875
AdeI CACNNNGTG 2 cut(s) 309, 375
AfaI GTAC 6 cut(s) 845, 923, 1293, 1359, 1424, 1920
AfiI CCNNNNNNNGG 6 cut(s) 249, 442, 443, 1036, 1319, 1702
AgsI TTSAA 7 cut(s) 104, 942, 1276, 1342, 1489, 1637, 1748
AjnI CCWGG 8 cut(s) 166, 322, 595, 793, 1035, 1228, 1531, 1570
AleI CACNNNNGTG 1 cut(s) 1739
AloI GAACNNNNNNTCC 2 cut(s) 1157, 1189
AluBI AGCT 9 cut(s) 59, 329, 542, 980, 1157, 1211, 1330, 1547, 1760
AluI AGCT 9 cut(s) 59, 329, 542, 980, 1157, 1211, 1330, 1547, 1760
Alw21I GWGCWC 2 cut(s) 559, 888
Alw26I GTCTC 2 cut(s) 571, 1804
AlwI GGATC 2 cut(s) 1450, 1469
AlwNI CAGNNNCTG 1 cut(s) 998
Ama87I CYCGRG 1 cut(s) 1296
Aor13HI TCCGGA 1 cut(s) 292
AoxI GGCC 9 cut(s) 6, 31, 110, 157, 589, 790, 796, 832, 874
ApaI GGGCCC 1 cut(s) 836
ApeKI GCWGC 7 cut(s) 71, 265, 539, 854, 932, 1154, 1211
ApoI RAATTY 2 cut(s) 92, 636
Asp700I GAANNNNTTC 1 cut(s) 630
Asp718I GGTACC 1 cut(s) 1422
AspS9I GGNCC 8 cut(s) 170, 290, 791, 832, 833, 1133, 1529, 1655
AsuC2I CCSGG 3 cut(s) 1123, 1297, 1298
AsuHPI GGTGA 6 cut(s) 127, 232, 611, 1231, 1302, 1802
AsuII TTCGAA 1 cut(s) 634
AsuNHI GCTAGC 1 cut(s) 1157
AvaI CYCGRG 1 cut(s) 1296
AvaII GGWCC 5 cut(s) 170, 290, 1133, 1529, 1655
BaeGI GKGCMC 3 cut(s) 314, 371, 836
BalI TGGCCA 2 cut(s) 159, 798
BanI GGYRCC 1 cut(s) 1422
BanII GRGCYC 3 cut(s) 424, 836, 1905
BauI CACGAG 1 cut(s) 858
BbsI GAAGAC 1 cut(s) 1228
Bbv12I GWGCWC 2 cut(s) 559, 888
BbvCI CCTCAGC 1 cut(s) 1761
BbvI GCAGC 7 cut(s) 58, 277, 551, 841, 919, 1166, 1198
BccI CCATC 6 cut(s) 251, 281, 390, 1410, 1526, 1533
BceAI ACGGC 2 cut(s) 191, 604
BciT130I CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1230, 1533, 1572
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 3 cut(s) 1123, 1297, 1298
BcoDI GTCTC 2 cut(s) 571, 1804
BfaI CTAG 2 cut(s) 438, 1158
BfmI CTRYAG 1 cut(s) 72
BglII AGATCT 1 cut(s) 1687
BisI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
BlsI GCNGC 7 cut(s) 73, 267, 541, 856, 934, 1156, 1213
Bme18I GGWCC 5 cut(s) 170, 290, 1133, 1529, 1655
BmeT110I CYCGRG 1 cut(s) 1296
BmgT120I GGNCC 8 cut(s) 170, 290, 791, 832, 833, 1133, 1529, 1655
BmiI GGNNCC 4 cut(s) 421, 834, 1424, 1848
BmsI GCATC 4 cut(s) 456, 758, 1420, 1575
BmtI GCTAGC 1 cut(s) 1161
BpiI GAAGAC 1 cut(s) 1228
BplI GAGNNNNNCTC 2 cut(s) 1784, 1816
BpmI CTGGAG 1 cut(s) 1668
Bpu10I CCTNAGC 1 cut(s) 1761
Bpu14I TTCGAA 1 cut(s) 634
BpuEI CTTGAG 2 cut(s) 550, 938
BpuMI CCSGG 3 cut(s) 1123, 1297, 1298
BsaAI YACGTR 1 cut(s) 1435
BsaBI GATNNNNATC 1 cut(s) 1605
BsaWI WCCGGW 2 cut(s) 292, 1283
BsaXI ACNNNNNCTCC 4 cut(s) 186, 216, 1527, 1557
Bsc4I CCNNNNNNNGG 6 cut(s) 249, 442, 443, 1036, 1319, 1702
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 5 cut(s) 160, 254, 641, 877, 959
Bse3DI GCAATG 1 cut(s) 971
Bse8I GATNNNNATC 1 cut(s) 1605
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1230, 1533, 1572
BseGI GGATG 5 cut(s) 243, 1402, 1544, 1729, 1801
BseJI GATNNNNATC 1 cut(s) 1605
BseLI CCNNNNNNNGG 6 cut(s) 249, 442, 443, 1036, 1319, 1702
BseMI GCAATG 1 cut(s) 971
BseMII CTCAG 6 cut(s) 415, 659, 1031, 1752, 1812, 1883
BseNI ACTGG 5 cut(s) 160, 254, 641, 877, 959
BseRI GAGGAG 4 cut(s) 133, 567, 1668, 1671
BseSI GKGCMC 3 cut(s) 314, 371, 836
BseXI GCAGC 7 cut(s) 58, 277, 551, 841, 919, 1166, 1198
BseYI CCCAGC 1 cut(s) 1466
BsgI GTGCAG 2 cut(s) 148, 1087
BshFI GGCC 9 cut(s) 8, 33, 112, 159, 591, 792, 798, 834, 876
BshNI GGYRCC 1 cut(s) 1422
BsiHKAI GWGCWC 2 cut(s) 559, 888
BsiHKCI CYCGRG 1 cut(s) 1296
BsiSI CCGG 5 cut(s) 113, 293, 1123, 1284, 1297
BslFI GGGAC 3 cut(s) 1031, 1211, 1829
BslI CCNNNNNNNGG 6 cut(s) 249, 442, 443, 1036, 1319, 1702
BsmAI GTCTC 2 cut(s) 571, 1804
BsmFI GGGAC 3 cut(s) 1031, 1211, 1829
BsmI GAATGC 1 cut(s) 934
BsnI GGCC 9 cut(s) 8, 33, 112, 159, 591, 792, 798, 834, 876
BsoBI CYCGRG 1 cut(s) 1296
Bsp119I TTCGAA 1 cut(s) 634
Bsp120I GGGCCC 1 cut(s) 832
Bsp1286I GDGCHC 7 cut(s) 314, 371, 424, 559, 836, 888, 1905
Bsp13I TCCGGA 1 cut(s) 292
Bsp1407I TGTACA 1 cut(s) 1357
Bsp143I GATC 8 cut(s) 79, 508, 601, 826, 1442, 1474, 1687, 1697
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 4 cut(s) 684, 1147, 1502, 1844
BspANI GGCC 9 cut(s) 8, 33, 112, 159, 591, 792, 798, 834, 876
BspCNI CTCAG 6 cut(s) 414, 660, 1030, 1753, 1813, 1882
BspEI TCCGGA 1 cut(s) 292
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 4 cut(s) 421, 834, 1424, 1848
BspMAI CTGCAG 1 cut(s) 76
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 2 cut(s) 1450, 1469
BspQI GCTCTTC 1 cut(s) 893
BspT104I TTCGAA 1 cut(s) 634
BspT107I GGYRCC 1 cut(s) 1422
BsrDI GCAATG 1 cut(s) 971
BsrFI RCCGGY 1 cut(s) 112
BsrGI TGTACA 1 cut(s) 1357
BsrI ACTGG 5 cut(s) 160, 254, 641, 877, 959
BssAI RCCGGY 1 cut(s) 112
BssMI GATC 8 cut(s) 79, 508, 601, 826, 1442, 1474, 1687, 1697
BssSI CACGAG 1 cut(s) 858
BssT1I CCWWGG 2 cut(s) 278, 1850
Bst2BI CACGAG 1 cut(s) 858
Bst2UI CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1230, 1533, 1572
Bst4CI ACNGT 9 cut(s) 151, 309, 412, 712, 733, 1081, 1108, 1592, 1665
Bst6I CTCTTC 3 cut(s) 707, 893, 1616
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 1357
BstBAI YACGTR 1 cut(s) 1435
BstBI TTCGAA 1 cut(s) 634
BstC8I GCNNGC 6 cut(s) 384, 537, 593, 978, 1159, 1209
BstDEI CTNAG 9 cut(s) 55, 401, 668, 1017, 1326, 1597, 1761, 1821, 1869
BstDSI CCRYGG 1 cut(s) 278
BstEII GGTNACC 1 cut(s) 1308
BstENI CCTNNNNNAGG 1 cut(s) 1034
BstF5I GGATG 5 cut(s) 243, 1402, 1544, 1729, 1801
BstKTI GATC 8 cut(s) 82, 511, 604, 829, 1445, 1477, 1690, 1700
BstMAI GTCTC 2 cut(s) 571, 1804
BstMBI GATC 8 cut(s) 79, 508, 601, 826, 1442, 1474, 1687, 1697
BstMWI GCNNNNNNNGC 2 cut(s) 860, 1013
BstNI CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1230, 1533, 1572
BstNSI RCATGY 1 cut(s) 386
BstPI GGTNACC 1 cut(s) 1308
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 3 cut(s) 314, 371, 836
BstV1I GCAGC 7 cut(s) 58, 277, 551, 841, 919, 1166, 1198
BstV2I GAAGAC 1 cut(s) 1228
BstX2I RGATCY 2 cut(s) 1474, 1687
BstXI CCANNNNNNTGG 3 cut(s) 254, 1539, 1578
BstYI RGATCY 2 cut(s) 1474, 1687
BsuRI GGCC 9 cut(s) 8, 33, 112, 159, 591, 792, 798, 834, 876
BtgI CCRYGG 1 cut(s) 278
BtsCI GGATG 5 cut(s) 243, 1402, 1544, 1729, 1801
BtsI GCAGTG 2 cut(s) 906, 1011
BtsIMutI CAGTG 7 cut(s) 247, 314, 408, 906, 1011, 1077, 1588
Cac8I GCNNGC 6 cut(s) 384, 537, 593, 978, 1159, 1209
CaiI CAGNNNCTG 1 cut(s) 998
CciI TCATGA 1 cut(s) 783
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 8 cut(s) 170, 290, 791, 832, 833, 1133, 1529, 1655
Cfr9I CCCGGG 1 cut(s) 1296
CseI GACGC 1 cut(s) 755
Csp6I GTAC 6 cut(s) 844, 922, 1292, 1358, 1423, 1919
CspCI CAANNNNNGTGG 2 cut(s) 1039, 1074
CviAII CATG 8 cut(s) 89, 254, 279, 383, 784, 850, 1162, 1418
CviQI GTAC 6 cut(s) 844, 922, 1292, 1358, 1423, 1919
DdeI CTNAG 9 cut(s) 55, 401, 668, 1017, 1326, 1597, 1761, 1821, 1869
DpnI GATC 8 cut(s) 81, 510, 603, 828, 1444, 1476, 1689, 1699
DpnII GATC 8 cut(s) 79, 508, 601, 826, 1442, 1474, 1687, 1697
DraIII CACNNNGTG 2 cut(s) 309, 375
EaeI YGGCCR 3 cut(s) 157, 589, 796
Eam1104I CTCTTC 3 cut(s) 707, 893, 1616
EarI CTCTTC 3 cut(s) 707, 893, 1616
Eco130I CCWWGG 2 cut(s) 278, 1850
Eco147I AGGCCT 1 cut(s) 8
Eco24I GRGCYC 3 cut(s) 424, 836, 1905
Eco47I GGWCC 5 cut(s) 170, 290, 1133, 1529, 1655
Eco57I CTGAAG 1 cut(s) 1875
Eco88I CYCGRG 1 cut(s) 1296
Eco91I GGTNACC 1 cut(s) 1308
EcoNI CCTNNNNNAGG 1 cut(s) 1034
EcoO109I RGGNCCY 3 cut(s) 791, 832, 833
EcoO65I GGTNACC 1 cut(s) 1308
EcoRI GAATTC 2 cut(s) 92, 636
EcoRII CCWGG 8 cut(s) 166, 322, 595, 793, 1035, 1228, 1531, 1570
EcoT14I CCWWGG 2 cut(s) 278, 1850
EcoT22I ATGCAT 2 cut(s) 577, 1413
EcoT38I GRGCYC 3 cut(s) 424, 836, 1905
ErhI CCWWGG 2 cut(s) 278, 1850
FaeI CATG 8 cut(s) 92, 257, 282, 386, 787, 853, 1165, 1421
FalI AAGNNNNNCTT 2 cut(s) 471, 503
FaqI GGGAC 3 cut(s) 1031, 1211, 1829
FatI CATG 8 cut(s) 88, 253, 278, 382, 783, 849, 1161, 1417
FauI CCCGC 2 cut(s) 1154, 1509
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
FokI GGATG 5 cut(s) 230, 1389, 1551, 1736, 1808
FriOI GRGCYC 3 cut(s) 424, 836, 1905
Fsp4HI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
FspBI CTAG 2 cut(s) 438, 1158
GluI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
GsaI CCCAGC 1 cut(s) 1470
GsuI CTGGAG 1 cut(s) 1668
HaeIII GGCC 9 cut(s) 8, 33, 112, 159, 591, 792, 798, 834, 876
HapII CCGG 5 cut(s) 113, 293, 1123, 1284, 1297
HgaI GACGC 1 cut(s) 755
Hin1II CATG 8 cut(s) 92, 257, 282, 386, 787, 853, 1165, 1421
HincII GTYRAC 1 cut(s) 1875
HindII GTYRAC 1 cut(s) 1875
HinfI GANTC 8 cut(s) 144, 197, 656, 672, 1485, 1601, 1817, 1867
HpaII CCGG 5 cut(s) 113, 293, 1123, 1284, 1297
HphI GGTGA 6 cut(s) 127, 232, 611, 1231, 1302, 1802
Hpy166II GTNNAC 3 cut(s) 348, 415, 1875
Hpy188I TCNGA 9 cut(s) 188, 404, 870, 1020, 1523, 1702, 1804, 1822, 1894
Hpy8I GTNNAC 3 cut(s) 348, 415, 1875
Hpy99I CGWCG 1 cut(s) 1320
HpyAV CCTTC 6 cut(s) 32, 44, 52, 73, 469, 497
HpyCH4III ACNGT 9 cut(s) 151, 309, 412, 712, 733, 1081, 1108, 1592, 1665
HpyCH4IV ACGT 3 cut(s) 300, 475, 1434
HpyF10VI GCNNNNNNNGC 2 cut(s) 860, 1013
HpyF3I CTNAG 9 cut(s) 55, 401, 668, 1017, 1326, 1597, 1761, 1821, 1869
HpySE526I ACGT 3 cut(s) 300, 475, 1434
Hsp92II CATG 8 cut(s) 92, 257, 282, 386, 787, 853, 1165, 1421
Kpn2I TCCGGA 1 cut(s) 292
KpnI GGTACC 1 cut(s) 1426
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 8 cut(s) 79, 508, 601, 826, 1442, 1474, 1687, 1697
LguI GCTCTTC 1 cut(s) 893
LmnI GCTCC 4 cut(s) 326, 419, 554, 1846
Lsp1109I GCAGC 7 cut(s) 58, 277, 551, 841, 919, 1166, 1198
LweI GCATC 4 cut(s) 456, 758, 1420, 1575
MaeI CTAG 2 cut(s) 438, 1158
MaeII ACGT 3 cut(s) 300, 475, 1434
MaeIII GTNAC 6 cut(s) 214, 301, 657, 1308, 1387, 1592
MalI GATC 8 cut(s) 81, 510, 603, 828, 1444, 1476, 1689, 1699
MboI GATC 8 cut(s) 79, 508, 601, 826, 1442, 1474, 1687, 1697
MboII GAAGA 8 cut(s) 89, 482, 694, 880, 930, 1228, 1406, 1603
MflI RGATCY 2 cut(s) 1474, 1687
MhlI GDGCHC 7 cut(s) 314, 371, 424, 559, 836, 888, 1905
MlsI TGGCCA 2 cut(s) 159, 798
MluCI AATT 6 cut(s) 92, 636, 1271, 1276, 1427, 1671
MluNI TGGCCA 2 cut(s) 159, 798
MlyI GAGTC 4 cut(s) 665, 681, 1811, 1861
MmeI TCCRAC 2 cut(s) 343, 1762
Mox20I TGGCCA 2 cut(s) 159, 798
Mph1103I ATGCAT 2 cut(s) 577, 1413
MroI TCCGGA 1 cut(s) 292
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 2 cut(s) 159, 798
MseI TTAA 3 cut(s) 585, 1202, 1557
MslI CAYNNNNRTG 4 cut(s) 87, 252, 1416, 1739
Msp20I TGGCCA 2 cut(s) 159, 798
MspI CCGG 5 cut(s) 113, 293, 1123, 1284, 1297
Mva1269I GAATGC 1 cut(s) 934
MvaI CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1230, 1533, 1572
MwoI GCNNNNNNNGC 2 cut(s) 860, 1013
NciI CCSGG 3 cut(s) 1123, 1297, 1298
NcoI CCATGG 1 cut(s) 278
NdeII GATC 8 cut(s) 79, 508, 601, 826, 1442, 1474, 1687, 1697
NheI GCTAGC 1 cut(s) 1157
NlaIII CATG 8 cut(s) 92, 257, 282, 386, 787, 853, 1165, 1421
NlaIV GGNNCC 4 cut(s) 421, 834, 1424, 1848
NmuCI GTSAC 5 cut(s) 301, 657, 1308, 1387, 1592
NsiI ATGCAT 2 cut(s) 577, 1413
NspI RCATGY 1 cut(s) 386
NspV TTCGAA 1 cut(s) 634
OliI CACNNNNGTG 1 cut(s) 1739
PaeI GCATGC 1 cut(s) 386
PagI TCATGA 1 cut(s) 783
PceI AGGCCT 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 893
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PctI GAATGC 1 cut(s) 934
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 4 cut(s) 144, 197, 1485, 1601
PflFI GACNNNGTC 1 cut(s) 1870
PflMI CCANNNNNTGG 1 cut(s) 249
PfoI TCCNGGA 1 cut(s) 1531
PkrI GCNGC 7 cut(s) 73, 267, 541, 856, 934, 1156, 1213
PleI GAGTC 4 cut(s) 664, 680, 1811, 1861
PpsI GAGTC 4 cut(s) 664, 680, 1811, 1861
Ppu21I YACGTR 1 cut(s) 1435
PsiI TTATAA 1 cut(s) 1098
Psp6I CCWGG 8 cut(s) 166, 322, 595, 793, 1035, 1228, 1531, 1570
PspEI GGTNACC 1 cut(s) 1308
PspFI CCCAGC 1 cut(s) 1466
PspGI CCWGG 8 cut(s) 166, 322, 595, 793, 1035, 1228, 1531, 1570
PspN4I GGNNCC 4 cut(s) 421, 834, 1424, 1848
PspOMI GGGCCC 1 cut(s) 832
PspPI GGNCC 8 cut(s) 170, 290, 791, 832, 833, 1133, 1529, 1655
PstI CTGCAG 1 cut(s) 76
PstNI CAGNNNCTG 1 cut(s) 998
PsuI RGATCY 2 cut(s) 1474, 1687
PsyI GACNNNGTC 1 cut(s) 1870
RsaI GTAC 6 cut(s) 845, 923, 1293, 1359, 1424, 1920
RsaNI GTAC 6 cut(s) 844, 922, 1292, 1358, 1423, 1919
RseI CAYNNNNRTG 4 cut(s) 87, 252, 1416, 1739
SapI GCTCTTC 1 cut(s) 893
SaqAI TTAA 3 cut(s) 585, 1202, 1557
SatI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
Sau3AI GATC 8 cut(s) 79, 508, 601, 826, 1442, 1474, 1687, 1697
Sau96I GGNCC 8 cut(s) 170, 290, 791, 832, 833, 1133, 1529, 1655
SchI GAGTC 4 cut(s) 665, 681, 1811, 1861
SduI GDGCHC 7 cut(s) 314, 371, 424, 559, 836, 888, 1905
SfaNI GCATC 4 cut(s) 456, 758, 1420, 1575
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 634
SinI GGWCC 5 cut(s) 170, 290, 1133, 1529, 1655
SmaI CCCGGG 1 cut(s) 1298
SmiMI CAYNNNNRTG 4 cut(s) 87, 252, 1416, 1739
SmlI CTYRAG 2 cut(s) 529, 917
SmoI CTYRAG 2 cut(s) 529, 917
SphI GCATGC 1 cut(s) 386
Sse9I AATT 6 cut(s) 92, 636, 1271, 1276, 1427, 1671
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 4 cut(s) 684, 1147, 1502, 1844
SspMI CTAG 2 cut(s) 438, 1158
StuI AGGCCT 1 cut(s) 8
StyI CCWWGG 2 cut(s) 278, 1850
TaaI ACNGT 9 cut(s) 151, 309, 412, 712, 733, 1081, 1108, 1592, 1665
TaiI ACGT 3 cut(s) 303, 478, 1437
TaqI TCGA 3 cut(s) 511, 634, 891
TasI AATT 6 cut(s) 92, 636, 1271, 1276, 1427, 1671
TatI WGTACW 2 cut(s) 921, 1357
TfiI GAWTC 4 cut(s) 144, 197, 1485, 1601
Tru1I TTAA 3 cut(s) 585, 1202, 1557
Tru9I TTAA 3 cut(s) 585, 1202, 1557
TscAI CASTG 7 cut(s) 254, 314, 415, 913, 1018, 1084, 1595
TseFI GTSAC 5 cut(s) 301, 657, 1308, 1387, 1592
TseI GCWGC 7 cut(s) 71, 265, 539, 854, 932, 1154, 1211
Tsp45I GTSAC 5 cut(s) 301, 657, 1308, 1387, 1592
TspDTI ATGAA 3 cut(s) 105, 1178, 1406
TspGWI ACGGA 4 cut(s) 190, 432, 1662, 1668
TspMI CCCGGG 1 cut(s) 1296
TspRI CASTG 7 cut(s) 254, 314, 415, 913, 1018, 1084, 1595
Tth111I GACNNNGTC 1 cut(s) 1870
Van91I CCANNNNNTGG 1 cut(s) 249
VpaK11BI GGWCC 5 cut(s) 170, 290, 1133, 1529, 1655
XagI CCTNNNNNAGG 1 cut(s) 1034
XapI RAATTY 2 cut(s) 92, 636
XceI RCATGY 1 cut(s) 386
XmaI CCCGGG 1 cut(s) 1296
XmiI GTMKAC 1 cut(s) 414
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 2 cut(s) 438, 1158
Zsp2I ATGCAT 2 cut(s) 577, 1413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.