pycom11g11360

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008361
Physical Location & Seq
Forward (+)
9958136 .. 9967440
9305 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3048 bp
ATGGTTTTGAGAGAGACGTACTCAAAATGCTATTATTTCAAAAGTGGGATTCTCTGTAAACTCTTCATCATCTCATATTTTTATCAAAATTTTTATATCAACATTATAAAATATTGTGCCATCGAACCTTATCATTTTTCTCTTCTTACAAAATTTGTTAATTTTTTTTTTTTTCCAAAAAAGCCAACGAGCCGATTAATTTTGCTCACGCAGCTTATTATTGTTTTTGACCCTCATTTTCTGACTCTCGATCTTCACGGCCTTTGCTCCCTCCTCCCTCATCGATCTCTCAAAAAAAAAGAAAAAAAAAAAACTAGGGTTTCGACTTTCGAGCTCCGGACCCACCGACCTCCCCCACCTGCTCCCCAAAACCTCCACCTCCTCCGCGACGGCCTAGATACTTTGCTACTGCTTCTCCCTCTGCTGCTGCTGCCGCCGCCTCGAGGGTTTACAGATCTAAGCCAGCTCTCACTTCCCAGCTCAGCTTTCTCTCTCTTCACCATGTTTTGGAAGCTCACTTCTGCCGCCTCTCCTGTGGAGACAGTGCTAGACAAGGAAAATTTTACATTAGAGGAGCTTCTGGATGAAGAAGAGATTATCCAAGAATGCAAAGCCTTAAACACTCGTCTCATTAATTTTTTACGGGATAGAGCCCAGGTGGAGCAATTATTGCGTTATATTGTTGAAGAACCTCCTGAGGATGCTGAAAGCAAACGAGCCTTCAAGTTCCCCTTCATTGCTTGTGAGGTTTTTACTTGTGAAATTGATGTTATCTTAAAGACTTTGGTGGATGAAGAGGAGCTAATGAACTTACTTTTCTCTTTCTTGGAACCAAATCGTCCTCATAGTGCCCCGTTGGCTGGGTATTTTGGCAAGGTTGTTGTATGCCTGATGATACGGAAGACAGCCCCACTTATGAACTATGTTCAAGCCCATGAGGATGTTTTCCGTCAGCTAGTTGATTTGATAGGAATTACGTCCATCATGGAGGTTTTGGTTCGACTTGTTGGTGTGGACGACCATGTGTATCCCAATTTTATTGATGTGATGCAATGGTTAGCTGAAAGTAATCTGCTTGAAATGATCGTTGATAAGTTGAGCCCAACTAATCCTCCTGAAGTTCATGCTAATGCAGCCGAAACACTTTCTGCTATAACTCGAAATGCTCCATCGGCCCTAGCCAACAAACTTTCTAGCCCAAGTTTTGTTACAAGGATATTTGGTCACGCATTGGAAGATTCACATTCAAAGTCTGGTCTTGTACACTCGCTTTCTGTCTGCATCTCTTTGTTGGATCCGAAAAGATCAGCAGTTTCTTCTCCCTTGTTCCATTCTTTTCGGGGCCAACATATGTATGAGTCTCCAATCCCTGTTAATCCAGATACCGTCAGTGCAATGCTGCCAAAACTTGGTGACTTGCTTGTGCTTTTGAATGTGTCATGGGATGAGAAAACATTGCCGACAACATACGGAGAGTTGAGGCCTCCACTTGGAAAGCATCGTTTAAAGATTGTGGAGTTCATTGCTGTTCTACTGAGATCGATCAATGAAGAGGCAGAAAAGGAATTGGTTAGCTCAGGAACCATCCAACGGGTCATTGATCTTTTCTTTGAGTACCCTTACAATAATTCCTTGCATCATCATGTAGATAGTATAATATCGTCATGTTTGGAAAGCAAGAGTGATGCCATTATTGACCATCTTCTTCGAGAGTGTGATTTAATTGGAAAGTTCCTTCAAACGGATAAAAATCCCATTCTATCTGGTGATGCTAATAAGCCAACGGTACCTACTACTGGAAAATCAGCACCACGAGCAGGAAACCTCGGGCACATCACAAGAATTTCTAATAAGCTCATTCAGTTGGGAAACAGCCAAAGCCGTGTTCAGGCATGCCTTCAGGAAAATAGCGACTGGAATGGGTGGCAAACAACAGTTTTGCAGGAGCGCAATGCAGTTGAAAATGTTTATCGATGGGCTTGCGGTCGCCCAACTGCTTTGCAAGATCGGACAAGGGATAGTGACGAGGATGACATGAATGACAGAGATTATGATGTAGCAGCTCTAGCAAATAATCTAAGCCAGGCTTTTAGATACAAAATATATGGGAATGAGGATGCAGGAGAGGACCAAGGAGCTCTTGATCGAGATGATGAGGATGTCTACTTTGATGATGAATCTGCTGAAGTGGTCATATCATCCTTGCGTCTTGGTGATGACCAAGGAAGCAGTTTATTCACAAATTCCAACTGGTTTGCATTCCAAGATGATAGAACCGGTGGTGAATCTGTGGGCACATCACTGTCAGAGATGGAAGAAGTAAACTTGAACTGCACTGGTGGTAACAGCAGTAGTGATGATGAGGTTGTGGTAGGGGAGGATGATAAGTTGGCCGGGAGTAAAGAGGCTGTCAATGGCACGTCCAGTTCCAACAGAAACCTTATGAATGGATTTAGCAACAGTGGTAACATGAACCCAGATGGCGAGAAGACAAGTGCCTCCAGTGACATGGGGGGTTTCTTCAGATTTGAGACATCTGATAATGATGACATGTTTGGAGACAGGCCTTTGCCTGAATGGGTGGGATGGAGTGAATCATCAGATTTGCAAGTCGGCGGCGCAAGTGTAAATCCATTTGAGGATCATAATGACGTAGTTGTCAATCCTTCAATGGCCGAGGTAGTGGCACCAGATGCTGGTGCTAGTTTGCACTCAAGTGGAGAATCTACACTTCCAAATGGCTCGCCTCCATCCTCCACTGCTTCTACTGCAGGGTCAGTGGGCAGTGGTGTGAGCCAGAGATCAGGTGCTGTCCCGTCACTGTTTGAAGAGGATGTTGAATTCGTGGGTGTGGAATTGGAAGGTACTGAGAAGGCTATGGAGCAGGCTCTTAAAGAGGGCATAGTAGGGGAGGCAGGGCCATTGAAGAAAAACATTATGCCGAAGGTACCAGAAAAAGAGAACCCTGATGACGGTGAGGCTGCAAACAAGGAGTTCAATGACACCAACTATTGGAGGGTTGATCAGGAAGTGACTGTTTTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

1016

Amino Acids

112.84

Weight (kDa)

4.8

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 296 - 656 7.4e-66 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 107
AarI CACCTGC 1 cut(s) 367
AasI GACNNNNNNGTC 1 cut(s) 2657
AbsI CCTCGAGG 1 cut(s) 441
Acc36I ACCTGC 1 cut(s) 367
Acc65I GGTACC 2 cut(s) 1786, 2947
AccB1I GGYRCC 3 cut(s) 1786, 2686, 2947
AccB7I CCANNNNNTGG 4 cut(s) 507, 1409, 2696, 3012
AccI GTMKAC 1 cut(s) 2163
AccII CGCG 1 cut(s) 387
AccIII TCCGGA 1 cut(s) 336
AciI CCGC 6 cut(s) 385, 434, 437, 525, 1983, 2616
AclWI GGATC 3 cut(s) 1289, 1302, 2649
AcoI YGGCCR 2 cut(s) 2391, 2673
AcsI RAATTY 6 cut(s) 88, 152, 559, 1842, 2242, 2840
AcuI CTGAAG 4 cut(s) 1137, 1883, 2205, 2506
AfaI GTAC 6 cut(s) 20, 1263, 1616, 1788, 2866, 2949
AflIII ACRYGT 1 cut(s) 2550
AgeI ACCGGT 1 cut(s) 2276
AjiI CACGTC 1 cut(s) 2421
AjnI CCWGG 2 cut(s) 654, 2082
AjuI GAANNNNNNNTTGG 2 cut(s) 1869, 1901
AleI CACNNNNGTG 1 cut(s) 2715
Alw21I GWGCWC 2 cut(s) 336, 2140
Alw26I GTCTC 6 cut(s) 8, 533, 632, 1365, 2525, 2553
AlwI GGATC 3 cut(s) 1289, 1302, 2649
AlwNI CAGNNNCTG 2 cut(s) 2696, 2810
Ama87I CYCGRG 2 cut(s) 441, 1826
Aor13HI TCCGGA 1 cut(s) 336
AoxI GGCC 9 cut(s) 259, 391, 1173, 1342, 1481, 2391, 2564, 2673, 2918
ApeKI GCWGC 8 cut(s) 211, 424, 427, 430, 1133, 1399, 2060, 2981
ApoI RAATTY 6 cut(s) 88, 152, 559, 1842, 2242, 2840
AseI ATTAAT 2 cut(s) 197, 633
AsiGI ACCGGT 1 cut(s) 2276
Asp718I GGTACC 2 cut(s) 1786, 2947
AspLEI GCGC 2 cut(s) 1950, 2621
AspS9I GGNCC 5 cut(s) 339, 1174, 1342, 2128, 2918
AsuC2I CCSGG 1 cut(s) 2395
AsuHPI GGTGA 6 cut(s) 490, 1424, 1778, 2225, 2294, 2987
AvaI CYCGRG 2 cut(s) 441, 1826
AvaII GGWCC 2 cut(s) 339, 2128
AxyI CCTNAGG 1 cut(s) 696
BaeGI GKGCMC 3 cut(s) 853, 1833, 2297
BamHI GGATCC 1 cut(s) 1294
BanI GGYRCC 3 cut(s) 1786, 2686, 2947
BanII GRGCYC 4 cut(s) 336, 655, 1103, 2140
BauI CACGAG 1 cut(s) 1812
BbsI GAAGAC 2 cut(s) 908, 2495
Bbv12I GWGCWC 2 cut(s) 336, 2140
BbvI GCAGC 8 cut(s) 223, 411, 414, 417, 1145, 1386, 2072, 2968
BceAI ACGGC 3 cut(s) 274, 406, 1866
BcgI CGANNNNNNTGC 2 cut(s) 1962, 1996
BciT130I CCWGG 2 cut(s) 656, 2084
BciVI GTATCC 1 cut(s) 1038
BclI TGATCA 1 cut(s) 3022
BcnI CCSGG 1 cut(s) 2395
BcoDI GTCTC 6 cut(s) 8, 533, 632, 1365, 2525, 2553
BfaI CTAG 8 cut(s) 315, 395, 548, 956, 1178, 1194, 2066, 2703
BfmI CTRYAG 1 cut(s) 2769
BfuAI ACCTGC 1 cut(s) 367
BfuI GTATCC 1 cut(s) 1038
BglI GCCNNNNNGGC 1 cut(s) 857
BglII AGATCT 1 cut(s) 454
BlpI GCTNAGC 1 cut(s) 481
Bme1390I CCNGG 3 cut(s) 656, 2084, 2395
Bme18I GGWCC 2 cut(s) 339, 2128
BmeT110I CYCGRG 2 cut(s) 441, 1826
BmgBI CACGTC 1 cut(s) 2421
BmgT120I GGNCC 5 cut(s) 339, 1174, 1342, 2128, 2918
BmiI GGNNCC 8 cut(s) 341, 831, 1296, 1343, 1582, 1788, 2688, 2949
BmrFI CCNGG 3 cut(s) 656, 2084, 2395
BmsI GCATC 9 cut(s) 691, 1038, 1290, 1507, 1645, 1675, 1759, 2107, 2683
BpiI GAAGAC 2 cut(s) 908, 2495
BplI GAGNNNNNCTC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 2485
Bpu10I CCTNAGC 1 cut(s) 1576
Bpu1102I GCTNAGC 1 cut(s) 481
BpuEI CTTGAG 1 cut(s) 2698
BpuMI CCSGG 1 cut(s) 2395
Bsa29I ATCGAT 3 cut(s) 283, 1541, 1972
BsaBI GATNNNNATC 1 cut(s) 1749
BsaJI CCNNGG 5 cut(s) 654, 1825, 2131, 2221, 2676
BsaWI WCCGGW 2 cut(s) 336, 2276
BsaXI ACNNNNNCTCC 4 cut(s) 399, 429, 1096, 1126
Bse118I RCCGGY 1 cut(s) 2276
Bse1I ACTGG 6 cut(s) 1801, 1919, 2255, 2341, 2424, 2502
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 6 cut(s) 735, 1058, 1401, 1454, 1521, 1957
Bse8I GATNNNNATC 1 cut(s) 1749
BseAI TCCGGA 1 cut(s) 336
BseBI CCWGG 2 cut(s) 656, 2084
BseCI ATCGAT 3 cut(s) 283, 1541, 1972
BseDI CCNNGG 5 cut(s) 654, 1825, 2131, 2221, 2676
BseJI GATNNNNATC 1 cut(s) 1749
BseMI GCAATG 6 cut(s) 735, 1058, 1401, 1454, 1521, 1957
BseMII CTCAG 5 cut(s) 495, 687, 1526, 1590, 2859
BseNI ACTGG 6 cut(s) 1801, 1919, 2255, 2341, 2424, 2502
BseRI GAGGAG 4 cut(s) 263, 371, 587, 812
BseSI GKGCMC 3 cut(s) 853, 1833, 2297
BseXI GCAGC 8 cut(s) 223, 411, 414, 417, 1145, 1386, 2072, 2968
BseYI CCCAGC 2 cut(s) 476, 860
BsgI GTGCAG 1 cut(s) 2317
Bsh1236I CGCG 1 cut(s) 387
Bsh1285I CGRYCG 1 cut(s) 1987
BshFI GGCC 9 cut(s) 261, 393, 1175, 1344, 1483, 2393, 2566, 2675, 2920
BshNI GGYRCC 3 cut(s) 1786, 2686, 2947
BshTI ACCGGT 1 cut(s) 2276
BshVI ATCGAT 3 cut(s) 283, 1541, 1972
BsiEI CGRYCG 1 cut(s) 1987
BsiHKAI GWGCWC 2 cut(s) 336, 2140
BsiHKCI CYCGRG 2 cut(s) 441, 1826
BsiSI CCGG 3 cut(s) 337, 2277, 2394
BslFI GGGAC 1 cut(s) 2798
BsmAI GTCTC 6 cut(s) 8, 533, 632, 1365, 2525, 2553
BsmBI CGTCTC 2 cut(s) 8, 632
BsmFI GGGAC 1 cut(s) 2798
BsmI GAATGC 2 cut(s) 611, 2258
BsnI GGCC 9 cut(s) 261, 393, 1175, 1344, 1483, 2393, 2566, 2675, 2920
BsoBI CYCGRG 2 cut(s) 441, 1826
Bsp1286I GDGCHC 7 cut(s) 336, 655, 853, 1103, 1833, 2140, 2297
Bsp13I TCCGGA 1 cut(s) 336
Bsp1407I TGTACA 1 cut(s) 1261
Bsp1720I GCTNAGC 1 cut(s) 481
BspACI CCGC 6 cut(s) 385, 434, 437, 525, 1983, 2616
BspANI GGCC 9 cut(s) 261, 393, 1175, 1344, 1483, 2393, 2566, 2675, 2920
BspCNI CTCAG 5 cut(s) 494, 688, 1527, 1589, 2860
BspDI ATCGAT 3 cut(s) 283, 1541, 1972
BspEI TCCGGA 1 cut(s) 336
BspFNI CGCG 1 cut(s) 387
BspLI GGNNCC 8 cut(s) 341, 831, 1296, 1343, 1582, 1788, 2688, 2949
BspMAI CTGCAG 1 cut(s) 2773
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 3 cut(s) 1289, 1302, 2649
BspT107I GGYRCC 3 cut(s) 1786, 2686, 2947
BsrDI GCAATG 6 cut(s) 735, 1058, 1401, 1454, 1521, 1957
BsrFI RCCGGY 1 cut(s) 2276
BsrGI TGTACA 1 cut(s) 1261
BsrI ACTGG 6 cut(s) 1801, 1919, 2255, 2341, 2424, 2502
BssAI RCCGGY 1 cut(s) 2276
BssECI CCNNGG 5 cut(s) 654, 1825, 2131, 2221, 2676
BssSI CACGAG 1 cut(s) 1812
BssT1I CCWWGG 2 cut(s) 2131, 2221
Bst2BI CACGAG 1 cut(s) 1812
Bst2UI CCWGG 2 cut(s) 656, 2084
Bst4CI ACNGT 9 cut(s) 544, 1387, 1786, 1936, 2304, 2462, 2823, 2975, 3037
Bst6I CTCTTC 7 cut(s) 68, 147, 500, 585, 789, 1545, 2823
BstAPI GCANNNNNTGC 2 cut(s) 670, 2693
BstAUI TGTACA 1 cut(s) 1261
BstC8I GCNNGC 5 cut(s) 464, 1894, 1981, 2744, 2886
BstDEI CTNAG 7 cut(s) 458, 481, 696, 1535, 1576, 2078, 2868
BstFNI CGCG 1 cut(s) 387
BstHHI GCGC 2 cut(s) 1950, 2621
BstMAI GTCTC 6 cut(s) 8, 533, 632, 1365, 2525, 2553
BstMCI CGRYCG 1 cut(s) 1987
BstNI CCWGG 2 cut(s) 656, 2084
BstNSI RCATGY 2 cut(s) 1896, 2554
BstSCI CCNGG 3 cut(s) 654, 2082, 2393
BstSFI CTRYAG 1 cut(s) 2769
BstSLI GKGCMC 3 cut(s) 853, 1833, 2297
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 8 cut(s) 223, 411, 414, 417, 1145, 1386, 2072, 2968
BstV2I GAAGAC 2 cut(s) 908, 2495
BstX2I RGATCY 2 cut(s) 454, 1294
BstXI CCANNNNNNTGG 1 cut(s) 2509
BstYI RGATCY 2 cut(s) 454, 1294
Bsu15I ATCGAT 3 cut(s) 283, 1541, 1972
Bsu36I CCTNAGG 1 cut(s) 696
BsuI GTATCC 1 cut(s) 1038
BsuRI GGCC 9 cut(s) 261, 393, 1175, 1344, 1483, 2393, 2566, 2675, 2920
BsuTUI ATCGAT 3 cut(s) 283, 1541, 1972
BtrI CACGTC 1 cut(s) 2421
BtsI GCAGTG 2 cut(s) 2757, 2791
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 5 cut(s) 464, 1894, 1981, 2744, 2886
CaiI CAGNNNCTG 2 cut(s) 2696, 2810
CfoI GCGC 2 cut(s) 1950, 2621
Cfr10I RCCGGY 1 cut(s) 2276
Cfr13I GGNCC 5 cut(s) 339, 1174, 1342, 2128, 2918
ClaI ATCGAT 3 cut(s) 283, 1541, 1972
CseI GACGC 1 cut(s) 2195
Csp6I GTAC 6 cut(s) 19, 1262, 1615, 1787, 2865, 2948
CspAI ACCGGT 1 cut(s) 2276
CviQI GTAC 6 cut(s) 19, 1262, 1615, 1787, 2865, 2948
DdeI CTNAG 7 cut(s) 458, 481, 696, 1535, 1576, 2078, 2868
DraI TTTAAA 1 cut(s) 1506
DrdI GACNNNNNNGTC 1 cut(s) 2657
DseDI GACNNNNNNGTC 1 cut(s) 2657
EaeI YGGCCR 2 cut(s) 2391, 2673
Eam1104I CTCTTC 7 cut(s) 68, 147, 500, 585, 789, 1545, 2823
EarI CTCTTC 7 cut(s) 68, 147, 500, 585, 789, 1545, 2823
Ecl136II GAGCTC 2 cut(s) 334, 2138
Eco130I CCWWGG 2 cut(s) 2131, 2221
Eco147I AGGCCT 2 cut(s) 1483, 2566
Eco24I GRGCYC 4 cut(s) 336, 655, 1103, 2140
Eco47I GGWCC 2 cut(s) 339, 2128
Eco53kI GAGCTC 2 cut(s) 334, 2138
Eco57I CTGAAG 4 cut(s) 1137, 1883, 2205, 2506
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 2 cut(s) 441, 1826
EcoICRI GAGCTC 2 cut(s) 334, 2138
EcoRI GAATTC 1 cut(s) 2840
EcoRII CCWGG 2 cut(s) 654, 2082
EcoT14I CCWWGG 2 cut(s) 2131, 2221
EcoT38I GRGCYC 4 cut(s) 336, 655, 1103, 2140
ErhI CCWWGG 2 cut(s) 2131, 2221
Esp3I CGTCTC 2 cut(s) 8, 632
FalI AAGNNNNNCTT 6 cut(s) 716, 748, 2071, 2103, 2124, 2156
FaqI GGGAC 1 cut(s) 2798
FauNDI CATATG 1 cut(s) 1350
FbaI TGATCA 1 cut(s) 3022
FblI GTMKAC 1 cut(s) 2163
FriOI GRGCYC 4 cut(s) 336, 655, 1103, 2140
FspBI CTAG 8 cut(s) 315, 395, 548, 956, 1178, 1194, 2066, 2703
GlaI GCGC 2 cut(s) 1949, 2620
GsaI CCCAGC 2 cut(s) 480, 864
GsuI CTGGAG 1 cut(s) 2485
HaeIII GGCC 9 cut(s) 261, 393, 1175, 1344, 1483, 2393, 2566, 2675, 2920
HapII CCGG 3 cut(s) 337, 2277, 2394
HgaI GACGC 1 cut(s) 2195
HhaI GCGC 2 cut(s) 1950, 2621
Hin6I GCGC 2 cut(s) 1948, 2619
HinP1I GCGC 2 cut(s) 1948, 2619
HinfI GANTC 8 cut(s) 49, 244, 1238, 1358, 2177, 2285, 2594, 2723
HpaII CCGG 3 cut(s) 337, 2277, 2394
HphI GGTGA 6 cut(s) 490, 1424, 1778, 2225, 2294, 2987
Hpy166II GTNNAC 6 cut(s) 59, 450, 1015, 1264, 2164, 2323
Hpy188I TCNGA 7 cut(s) 243, 1299, 2010, 2308, 2525, 2539, 2602
Hpy8I GTNNAC 6 cut(s) 59, 450, 1015, 1264, 2164, 2323
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 8 cut(s) 730, 742, 1745, 1907, 2676, 2855, 2866, 2938
HpyCH4III ACNGT 9 cut(s) 544, 1387, 1786, 1936, 2304, 2462, 2823, 2975, 3037
HpyCH4IV ACGT 4 cut(s) 17, 977, 2420, 2652
HpyF3I CTNAG 7 cut(s) 458, 481, 696, 1535, 1576, 2078, 2868
HpySE526I ACGT 4 cut(s) 17, 977, 2420, 2652
HspAI GCGC 2 cut(s) 1948, 2619
Kpn2I TCCGGA 1 cut(s) 336
KpnI GGTACC 2 cut(s) 1790, 2951
Ksp22I TGATCA 1 cut(s) 3022
Lsp1109I GCAGC 8 cut(s) 223, 411, 414, 417, 1145, 1386, 2072, 2968
LweI GCATC 9 cut(s) 691, 1038, 1290, 1507, 1645, 1675, 1759, 2107, 2683
MaeI CTAG 8 cut(s) 315, 395, 548, 956, 1178, 1194, 2066, 2703
MaeII ACGT 4 cut(s) 17, 977, 2420, 2652
MaeIII GTNAC 9 cut(s) 1207, 1223, 1412, 2021, 2342, 2465, 2504, 2817, 3031
MflI RGATCY 2 cut(s) 454, 1294
MhlI GDGCHC 7 cut(s) 336, 655, 853, 1103, 1833, 2140, 2297
MlyI GAGTC 2 cut(s) 238, 1367
MmeI TCCRAC 4 cut(s) 1272, 1612, 2271, 2454
MroI TCCGGA 1 cut(s) 336
MseI TTAA 9 cut(s) 159, 197, 617, 633, 776, 1374, 1505, 1721, 2892
MslI CAYNNNNRTG 5 cut(s) 939, 1128, 1353, 1641, 2715
MspI CCGG 3 cut(s) 337, 2277, 2394
MspR9I CCNGG 3 cut(s) 656, 2084, 2395
Mva1269I GAATGC 2 cut(s) 611, 2258
MvaI CCWGG 2 cut(s) 656, 2084
MvnI CGCG 1 cut(s) 387
NciI CCSGG 1 cut(s) 2395
NdeI CATATG 1 cut(s) 1350
NlaIV GGNNCC 8 cut(s) 341, 831, 1296, 1343, 1582, 1788, 2688, 2949
NmeAIII GCCGAG 1 cut(s) 2701
NmuCI GTSAC 6 cut(s) 1223, 1412, 2021, 2504, 2817, 3031
NspI RCATGY 2 cut(s) 1896, 2554
OliI CACNNNNGTG 1 cut(s) 2715
PaeI GCATGC 1 cut(s) 1896
PaeR7I CTCGAG 1 cut(s) 441
PaqCI CACCTGC 1 cut(s) 367
PceI AGGCCT 2 cut(s) 1483, 2566
PciI ACATGT 1 cut(s) 2550
PctI GAATGC 2 cut(s) 611, 2258
PfeI GAWTC 6 cut(s) 49, 1238, 2177, 2285, 2594, 2723
PflMI CCANNNNNTGG 4 cut(s) 507, 1409, 2696, 3012
PinAI ACCGGT 1 cut(s) 2276
PleI GAGTC 2 cut(s) 238, 1366
PpsI GAGTC 2 cut(s) 238, 1366
PscI ACATGT 1 cut(s) 2550
PshBI ATTAAT 2 cut(s) 197, 633
PsiI TTATAA 1 cut(s) 107
Psp124BI GAGCTC 2 cut(s) 336, 2140
Psp6I CCWGG 2 cut(s) 654, 2082
PspFI CCCAGC 2 cut(s) 476, 860
PspGI CCWGG 2 cut(s) 654, 2082
PspN4I GGNNCC 8 cut(s) 341, 831, 1296, 1343, 1582, 1788, 2688, 2949
PspPI GGNCC 5 cut(s) 339, 1174, 1342, 2128, 2918
PspXI VCTCGAGB 1 cut(s) 441
PstI CTGCAG 1 cut(s) 2773
PstNI CAGNNNCTG 2 cut(s) 2696, 2810
PsuI RGATCY 2 cut(s) 454, 1294
RsaI GTAC 6 cut(s) 20, 1263, 1616, 1788, 2866, 2949
RsaNI GTAC 6 cut(s) 19, 1262, 1615, 1787, 2865, 2948
RseI CAYNNNNRTG 5 cut(s) 939, 1128, 1353, 1641, 2715
SacI GAGCTC 2 cut(s) 336, 2140
SaqAI TTAA 9 cut(s) 159, 197, 617, 633, 776, 1374, 1505, 1721, 2892
Sau96I GGNCC 5 cut(s) 339, 1174, 1342, 2128, 2918
SchI GAGTC 2 cut(s) 238, 1367
ScrFI CCNGG 3 cut(s) 656, 2084, 2395
SduI GDGCHC 7 cut(s) 336, 655, 853, 1103, 1833, 2140, 2297
SfaNI GCATC 9 cut(s) 691, 1038, 1290, 1507, 1645, 1675, 1759, 2107, 2683
SfcI CTRYAG 1 cut(s) 2769
Sfr274I CTCGAG 1 cut(s) 441
SinI GGWCC 2 cut(s) 339, 2128
SlaI CTCGAG 1 cut(s) 441
SmiMI CAYNNNNRTG 5 cut(s) 939, 1128, 1353, 1641, 2715
SmlI CTYRAG 2 cut(s) 441, 2713
SmoI CTYRAG 2 cut(s) 441, 2713
SphI GCATGC 1 cut(s) 1896
SseBI AGGCCT 2 cut(s) 1483, 2566
SsiI CCGC 6 cut(s) 385, 434, 437, 525, 1983, 2616
SspI AATATT 1 cut(s) 113
SspMI CTAG 8 cut(s) 315, 395, 548, 956, 1178, 1194, 2066, 2703
SstI GAGCTC 2 cut(s) 336, 2140
StuI AGGCCT 2 cut(s) 1483, 2566
StyD4I CCNGG 3 cut(s) 654, 2082, 2393
StyI CCWWGG 2 cut(s) 2131, 2221
TaaI ACNGT 9 cut(s) 544, 1387, 1786, 1936, 2304, 2462, 2823, 2975, 3037
TaiI ACGT 4 cut(s) 20, 980, 2423, 2655
TatI WGTACW 1 cut(s) 1261
TauI GCSGC 4 cut(s) 436, 439, 527, 2619
TfiI GAWTC 6 cut(s) 49, 1238, 2177, 2285, 2594, 2723
Tru1I TTAA 9 cut(s) 159, 197, 617, 633, 776, 1374, 1505, 1721, 2892
Tru9I TTAA 9 cut(s) 159, 197, 617, 633, 776, 1374, 1505, 1721, 2892
TseFI GTSAC 6 cut(s) 1223, 1412, 2021, 2504, 2817, 3031
TseI GCWGC 8 cut(s) 211, 424, 427, 430, 1133, 1399, 2060, 2981
Tsp45I GTSAC 6 cut(s) 1223, 1412, 2021, 2504, 2817, 3031
TspGWI ACGGA 4 cut(s) 913, 938, 1485, 1757
Van91I CCANNNNNTGG 4 cut(s) 507, 1409, 2696, 3012
VpaK11BI GGWCC 2 cut(s) 339, 2128
VspI ATTAAT 2 cut(s) 197, 633
XapI RAATTY 6 cut(s) 88, 152, 559, 1842, 2242, 2840
XceI RCATGY 2 cut(s) 1896, 2554
XhoI CTCGAG 1 cut(s) 441
XmiI GTMKAC 1 cut(s) 2163
XspI CTAG 8 cut(s) 315, 395, 548, 956, 1178, 1194, 2066, 2703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.