pycom11g27380

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005087
Physical Location & Seq
Forward (+)
29649510 .. 29654330
4821 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2469 bp
ATGAGCAGAACAGGGACGATGCTCTCCAAAGCTTCCGACTCTGCAAATAAACTCACAAAATTCCATGTAGAAGGGCCGAGCGATGGCGTTGGAAGCTTGGAGCAGAAGATGCACCAGCTCAAGTTGCAAATTCAAGCAGAGAGAGTGATTTCAGTAAAAGAAAAGGTTGAGAAGAACAGGGAGAAGGTAGAAGGTTATGTTTCGAAAATTATATCTGAGACAGCAAGAGCGAATTTGAATGCTTTGTCATCAGAGCGGAATAGGAGCTTTAAATTGTTTTCTTCAAGAATTGAACAGCCTCTCTGCAGAATCAGTGGATTTGTGCGGGGATGTGGTGAGAAAGACTGGATTGAGAATCAAGAGGTGTTATTCTCATCAAGTACCAAGCTTCCACTTGCTGACAAGATACCGCCTTATACAACTTGGATATTCTTGGACAGAAATCAAAGAATGGCTGAAGACCAATCAGTAGTTGGGAGGAGGCGCATTTATTACGATGAAGATGGCAGTGAAGCTCTTGTCTACAGTGGCACTGACGATGAGGCAGAAGAACCCGAAGAAGTAAAACATGAATTTTCTGCGGGCGAAGATCGAATTCTGTTGATGGCCTTTCAGGAGCATGGCCTAGGCGAGGAAGTAGTGGAACTTGTGAAGGAGTTTATTGGAGTTCCCATTTCGGATATCCTGGCCCGTTATAACACAATCAAGGAAAGGAACCCCGAGAAGTGCGAACATGAAGGGTGCATTTCTCTTGATAAGAGTCTTAATGCTGCCTTAGATTCTTTTGATAACCTTTTCTGTCGCCGTTGCTTGGTATTTGATTGTCGTTTGCATGGATGTTCTCAACCTTTAATCTATCCTAGTGAAAAATCACATTGGTCCGGTCATGACGAGAACCGAGAGCCATGCAGTGATCAATGTTACCTCAGGTTGAGTATAGTAACATCAGAGAGAGAAAGTGCTCCAGGATCATCCATTAATGCCGAGCGACCAAGTTCTCATGGTGGTACCGGCCTCTTACACAATGAAAGAAGTATTCCTGGTGAAGCTGTGCCTGCAACTTCAGAAGCCATTCATAGTTCTCATTTCCTGAAGATGCACAATGAAAACATAGGGAAGCGCAAGGTCGTGAAGCATACAGATAAAGTGGCAAATGACCTAACTATAGTACCTGATGCTTTCCAGGGTTCTTCTAAGAAACAAAAGAGATTAGATGCTTTGGATTTGGTAACTGCTACTAGTGAACCTATCCCGGTTCAGGTTCACATTTCCAGAGATGAGCCACGAGATGTTACTGAAGTGCCTGAACTGAGGCAAACATCCAAGTCTACATGTGAACAAGTTGAGGGGATCTATAGCAACTCTGAGTGGAAACCAGTGGAGAAAGATTTATACATGAAGGGACTGCAGATATTTGGGAGAAACAGTTGCCTCATAGCGAGGAACTTACTCTCTGGCTTGAGGACTTGCATGGAGGTGTCTAGTTACATGCATAATTCTGGAGCTTCAATGCCTAATAGATCTGTGGTTGGACCATCTTCATTTATGGAAGACAATGTGAAATCTGATATGGATCAGACAGAACAAGAGATGTCGTCAAAGCCACGGTTACTTCGTAGAAGGGGCAAAGCACGGAGGCTTAAGTATTCTTGGAAGTCTGCTGGGCACCCAACAATGTGGAAAAGAATTGCTGATGGCAAAAACGATTCACGTAAACTATATACGCCATGTGGATGCCAGTCTATGTGCGGAAAGGAATGTCCTTGTATGAGTAATGGAACCTGCTGTGAAAAATACTGTGGCTGTTCGAAGAGCTGCAAAAATAGGTTCAGGGGATGTCACTGTGCAAAGAGTCAATGTAGAAGTCGGCAGTGCCCATGCTTTGCTGCTGGCCGTGAATGTGACCCAGATGTCTGTCGTCATTGTTGGGTTAGTTGTGGGGATGGTTCACTAGGCGAGCCACCGAGACAGGGAGATAGTCAGTGTGGAAACATGAGGCTTCTTCTACGACAACAACAGAGGATTCTCCTGGGCAAGTCTGATGTTGCTGGCTGGGGAGCCTTCTTAAAGAACCCCGTAAACAAGAATGATTATCTTGGAGAATATACTGGTGAAATCATCTCCCATCAGGAAGCAGACAAGCGGGGGAAGATTTATGATCGTGTAAACTCGTCATTCCTTTTCGACTTGAATGAACAGTATGTCCTAGATGCTTACCGAAAGGGAGACAAGCTTAAATTTGCAAACCACTCGTCGAATCCTAACTGCCATGCAAAGGTAATGCTGGTCGCGGGTGATCATCGAGTTGGCATATTTGCCAAGGAGCATATTGATGCTGGTGAGGAGCTCTTCTATGATTATTGTTATCTTTCAGAGGCAGCACCTGTATGGGCTCAAAAACCGGAGGGTTCTAAGAGGGACGATTCATCTGCTTCCAGGGGCAGAGCGAAAAAGCACCAACCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0005911 GO:0006139 GO:0006325 GO:0006342 GO:0006349 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0030054 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048316 GO:0048317 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048587 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070734 GO:0071514 GO:0071704 GO:0071840 GO:0080050 GO:0080090 GO:0090568 GO:0090698 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:2000014 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00310 ko05206 map00310 map05206
Pfam Domains
Protein Families
SET

Protein Analysis

823

Amino Acids

92.4

Weight (kDa)

7.93

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_CLF_N PF25996 192 - 236 1.2e-17 Histone-lysine N-methyltransferase CLF, N-terminal HTH domain
preSET_CXC PF18264 612 - 643 3.5e-07 CXC domain
SET PF00856 684 - 787 2.2e-20 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AasI GACNNNNNNGTC 1 cut(s) 1910
Acc36I ACCTGC 1 cut(s) 1790
Acc65I GGTACC 1 cut(s) 1007
AccB1I GGYRCC 2 cut(s) 1007, 1665
AccBSI CCGCTC 1 cut(s) 256
AccI GTMKAC 2 cut(s) 522, 1328
AccII CGCG 1 cut(s) 2291
AciI CCGC 7 cut(s) 256, 325, 410, 581, 1749, 2143, 2291
AclWI GGATC 3 cut(s) 976, 1358, 1581
AcoI YGGCCR 1 cut(s) 1891
AcsI RAATTY 6 cut(s) 59, 129, 232, 572, 594, 2237
AcuI CTGAAG 4 cut(s) 477, 1047, 1112, 1317
AfaI GTAC 3 cut(s) 382, 1009, 1170
AfiI CCNNNNNNNGG 6 cut(s) 83, 631, 811, 1258, 1970, 2275
AflII CTTAAG 1 cut(s) 1640
AflIII ACRYGT 1 cut(s) 1331
AgsI TTSAA 6 cut(s) 134, 238, 285, 293, 1509, 2191
AhlI ACTAGT 1 cut(s) 1238
AjnI CCWGG 6 cut(s) 684, 964, 1039, 1182, 2028, 2435
AloI GAACNNNNNNTCC 2 cut(s) 2187, 2219
Alw21I GWGCWC 2 cut(s) 964, 2349
Alw26I GTCTC 3 cut(s) 212, 1960, 2220
AlwI GGATC 3 cut(s) 976, 1358, 1581
AlwNI CAGNNNCTG 1 cut(s) 2384
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 6 cut(s) 74, 606, 622, 687, 1012, 1891
ApeKI GCWGC 4 cut(s) 770, 1815, 1886, 2378
ApoI RAATTY 6 cut(s) 59, 129, 232, 572, 594, 2237
ArsI GACNNNNNNTTYG 2 cut(s) 2237, 2269
AseI ATTAAT 1 cut(s) 978
Asp700I GAANNNNTTC 1 cut(s) 1071
Asp718I GGTACC 1 cut(s) 1007
AspA2I CCTAGG 1 cut(s) 625
AspLEI GCGC 2 cut(s) 486, 1122
AspS9I GGNCC 4 cut(s) 74, 688, 879, 1532
AsuC2I CCSGG 1 cut(s) 1253
AsuHPI GGTGA 5 cut(s) 347, 1055, 2123, 2306, 2351
AsuII TTCGAA 2 cut(s) 203, 1808
AvaI CYCGRG 1 cut(s) 719
AvaII GGWCC 2 cut(s) 879, 1532
AvrII CCTAGG 1 cut(s) 625
AxyI CCTNAGG 1 cut(s) 926
BaeGI GKGCMC 2 cut(s) 1668, 1877
BaeI ACNNNNGTAYC 2 cut(s) 1152, 1185
BanI GGYRCC 2 cut(s) 1007, 1665
BanII GRGCYC 2 cut(s) 2349, 2395
BauI CACGAG 1 cut(s) 1284
BbsI GAAGAC 2 cut(s) 465, 1557
Bbv12I GWGCWC 2 cut(s) 964, 2349
BbvI GCAGC 4 cut(s) 757, 1802, 1873, 2390
BccI CCATC 7 cut(s) 77, 497, 598, 1543, 1688, 1937, 2133
BceAI ACGGC 2 cut(s) 789, 1878
BcgI CGANNNNNNTGC 6 cut(s) 888, 922, 2232, 2266, 2411, 2445
BciT130I CCWGG 6 cut(s) 686, 966, 1041, 1184, 2030, 2437
BclI TGATCA 2 cut(s) 913, 2296
BcnI CCSGG 1 cut(s) 1253
BcoDI GTCTC 3 cut(s) 212, 1960, 2220
BcuI ACTAGT 1 cut(s) 1238
BfaI CTAG 6 cut(s) 626, 861, 1239, 1482, 1952, 2207
BfmI CTRYAG 5 cut(s) 304, 523, 1164, 1354, 1406
BfrI CTTAAG 1 cut(s) 1640
BfuAI ACCTGC 1 cut(s) 1790
BglII AGATCT 1 cut(s) 1520
BisI GCNGC 4 cut(s) 771, 1816, 1887, 2379
BlnI CCTAGG 1 cut(s) 625
BlsI GCNGC 4 cut(s) 772, 1817, 1888, 2380
Bme1390I CCNGG 7 cut(s) 686, 966, 1041, 1184, 1253, 2030, 2437
Bme18I GGWCC 2 cut(s) 879, 1532
BmeT110I CYCGRG 1 cut(s) 719
BmgT120I GGNCC 4 cut(s) 74, 688, 879, 1532
BmiI GGNNCC 5 cut(s) 716, 1009, 1667, 1780, 2059
BmrFI CCNGG 7 cut(s) 686, 966, 1041, 1184, 1253, 2030, 2437
BmsI GCATC 8 cut(s) 9, 99, 1086, 1165, 1204, 1724, 2200, 2323
BpiI GAAGAC 2 cut(s) 465, 1557
BpmI CTGGAG 2 cut(s) 948, 1521
Bpu14I TTCGAA 2 cut(s) 203, 1808
BpuEI CTTGAG 2 cut(s) 104, 1480
BpuMI CCSGG 1 cut(s) 1253
BsaAI YACGTR 1 cut(s) 1712
BsaBI GATNNNNATC 1 cut(s) 1572
BsaJI CCNNGG 6 cut(s) 625, 1183, 1604, 2029, 2319, 2436
BsaWI WCCGGW 2 cut(s) 881, 2401
BsaXI ACNNNNNCTCC 2 cut(s) 1411, 1441
Bsc4I CCNNNNNNNGG 6 cut(s) 83, 631, 811, 1258, 1970, 2275
Bse118I RCCGGY 1 cut(s) 1010
Bse1I ACTGG 4 cut(s) 350, 1376, 1738, 2113
Bse21I CCTNAGG 1 cut(s) 926
Bse8I GATNNNNATC 1 cut(s) 1572
BseBI CCWGG 6 cut(s) 686, 966, 1041, 1184, 2030, 2437
BseDI CCNNGG 6 cut(s) 625, 1183, 1604, 2029, 2319, 2436
BseGI GGATG 7 cut(s) 335, 842, 971, 1319, 1739, 1841, 1948
BseJI GATNNNNATC 1 cut(s) 1572
BseLI CCNNNNNNNGG 6 cut(s) 83, 631, 811, 1258, 1970, 2275
BseMII CTCAG 4 cut(s) 207, 940, 1301, 1356
BseNI ACTGG 4 cut(s) 350, 1376, 1738, 2113
BseRI GAGGAG 2 cut(s) 493, 2357
BseSI GKGCMC 2 cut(s) 1668, 1877
BseXI GCAGC 4 cut(s) 757, 1802, 1873, 2390
BseYI CCCAGC 2 cut(s) 1661, 2052
Bsh1236I CGCG 1 cut(s) 2291
BshFI GGCC 6 cut(s) 76, 608, 624, 689, 1014, 1893
BshNI GGYRCC 2 cut(s) 1007, 1665
BsiHKAI GWGCWC 2 cut(s) 964, 2349
BsiHKCI CYCGRG 1 cut(s) 719
BsiSI CCGG 4 cut(s) 882, 1011, 1253, 2402
BslFI GGGAC 3 cut(s) 28, 1416, 2432
BslI CCNNNNNNNGG 6 cut(s) 83, 631, 811, 1258, 1970, 2275
BsmAI GTCTC 3 cut(s) 212, 1960, 2220
BsmFI GGGAC 3 cut(s) 28, 1416, 2432
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 6 cut(s) 76, 608, 624, 689, 1014, 1893
BsoBI CYCGRG 1 cut(s) 719
Bsp119I TTCGAA 2 cut(s) 203, 1808
Bsp1286I GDGCHC 5 cut(s) 964, 1668, 1877, 2349, 2395
Bsp143I GATC 8 cut(s) 589, 913, 968, 1350, 1520, 1573, 2158, 2296
BspACI CCGC 7 cut(s) 256, 325, 410, 581, 1749, 2143, 2291
BspANI GGCC 6 cut(s) 76, 608, 624, 689, 1014, 1893
BspCNI CTCAG 4 cut(s) 208, 939, 1302, 1357
BspFNI CGCG 1 cut(s) 2291
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 5 cut(s) 716, 1009, 1667, 1780, 2059
BspMAI CTGCAG 2 cut(s) 308, 1410
BspMI ACCTGC 1 cut(s) 1790
BspPI GGATC 3 cut(s) 976, 1358, 1581
BspQI GCTCTTC 2 cut(s) 1805, 2354
BspT104I TTCGAA 2 cut(s) 203, 1808
BspT107I GGYRCC 2 cut(s) 1007, 1665
BspTI CTTAAG 1 cut(s) 1640
BsrBI CCGCTC 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 1010
BsrI ACTGG 4 cut(s) 350, 1376, 1738, 2113
BssAI RCCGGY 1 cut(s) 1010
BssECI CCNNGG 6 cut(s) 625, 1183, 1604, 2029, 2319, 2436
BssMI GATC 8 cut(s) 589, 913, 968, 1350, 1520, 1573, 2158, 2296
BssSI CACGAG 1 cut(s) 1284
BssT1I CCWWGG 2 cut(s) 625, 2319
Bst2BI CACGAG 1 cut(s) 1284
Bst2UI CCWGG 6 cut(s) 686, 966, 1041, 1184, 2030, 2437
Bst4CI ACNGT 6 cut(s) 527, 1427, 1608, 1799, 1844, 2199
Bst6I CTCTTC 2 cut(s) 1805, 2354
BstAFI CTTAAG 1 cut(s) 1640
BstAPI GCANNNNNTGC 1 cut(s) 109
BstBAI YACGTR 1 cut(s) 1712
BstBI TTCGAA 2 cut(s) 203, 1808
BstC8I GCNNGC 5 cut(s) 583, 1056, 1891, 1958, 2050
BstDEI CTNAG 7 cut(s) 216, 775, 926, 1194, 1310, 1365, 2412
BstDSI CCRYGG 1 cut(s) 1604
BstENI CCTNNNNNAGG 1 cut(s) 629
BstF5I GGATG 7 cut(s) 335, 842, 971, 1319, 1739, 1841, 1948
BstFNI CGCG 1 cut(s) 2291
BstHHI GCGC 2 cut(s) 486, 1122
BstKTI GATC 8 cut(s) 592, 916, 971, 1353, 1523, 1576, 2161, 2299
BstMAI GTCTC 3 cut(s) 212, 1960, 2220
BstMBI GATC 8 cut(s) 589, 913, 968, 1350, 1520, 1573, 2158, 2296
BstMWI GCNNNNNNNGC 4 cut(s) 93, 109, 124, 1055
BstNI CCWGG 6 cut(s) 686, 966, 1041, 1184, 2030, 2437
BstNSI RCATGY 2 cut(s) 1335, 1492
BstSCI CCNGG 7 cut(s) 684, 964, 1039, 1182, 1251, 2028, 2435
BstSFI CTRYAG 5 cut(s) 304, 523, 1164, 1354, 1406
BstSLI GKGCMC 2 cut(s) 1668, 1877
BstUI CGCG 1 cut(s) 2291
BstV1I GCAGC 4 cut(s) 757, 1802, 1873, 2390
BstV2I GAAGAC 2 cut(s) 465, 1557
BstX2I RGATCY 2 cut(s) 1350, 1520
BstXI CCANNNNNNTGG 1 cut(s) 1677
BstYI RGATCY 2 cut(s) 1350, 1520
Bsu36I CCTNAGG 1 cut(s) 926
BsuRI GGCC 6 cut(s) 76, 608, 624, 689, 1014, 1893
BtgI CCRYGG 1 cut(s) 1604
BtgZI GCGATG 1 cut(s) 96
BtsCI GGATG 7 cut(s) 335, 842, 971, 1319, 1739, 1841, 1948
BtsI GCAGTG 3 cut(s) 514, 916, 1877
BtsIMutI CAGTG 9 cut(s) 319, 514, 531, 532, 916, 1383, 1840, 1877, 1988
BveI ACCTGC 1 cut(s) 1790
Cac8I GCNNGC 5 cut(s) 583, 1056, 1891, 1958, 2050
CaiI CAGNNNCTG 1 cut(s) 2384
CciI TCATGA 1 cut(s) 886
CfoI GCGC 2 cut(s) 486, 1122
Cfr10I RCCGGY 1 cut(s) 1010
Cfr13I GGNCC 4 cut(s) 74, 688, 879, 1532
Csp6I GTAC 3 cut(s) 381, 1008, 1169
CviQI GTAC 3 cut(s) 381, 1008, 1169
DdeI CTNAG 7 cut(s) 216, 775, 926, 1194, 1310, 1365, 2412
DpnI GATC 8 cut(s) 591, 915, 970, 1352, 1522, 1575, 2160, 2298
DpnII GATC 8 cut(s) 589, 913, 968, 1350, 1520, 1573, 2158, 2296
DraI TTTAAA 1 cut(s) 271
DrdI GACNNNNNNGTC 1 cut(s) 1910
DseDI GACNNNNNNGTC 1 cut(s) 1910
EaeI YGGCCR 1 cut(s) 1891
Eam1104I CTCTTC 2 cut(s) 1805, 2354
EarI CTCTTC 2 cut(s) 1805, 2354
Ecl136II GAGCTC 1 cut(s) 2347
Eco130I CCWWGG 2 cut(s) 625, 2319
Eco24I GRGCYC 2 cut(s) 2349, 2395
Eco32I GATATC 1 cut(s) 682
Eco47I GGWCC 2 cut(s) 879, 1532
Eco53kI GAGCTC 1 cut(s) 2347
Eco57I CTGAAG 4 cut(s) 477, 1047, 1112, 1317
Eco81I CCTNAGG 1 cut(s) 926
Eco88I CYCGRG 1 cut(s) 719
EcoICRI GAGCTC 1 cut(s) 2347
EcoNI CCTNNNNNAGG 1 cut(s) 629
EcoRI GAATTC 1 cut(s) 594
EcoRII CCWGG 6 cut(s) 684, 964, 1039, 1182, 2028, 2435
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 2 cut(s) 625, 2319
EcoT22I ATGCAT 1 cut(s) 1494
EcoT38I GRGCYC 2 cut(s) 2349, 2395
ErhI CCWWGG 2 cut(s) 625, 2319
FaqI GGGAC 3 cut(s) 28, 1416, 2432
FauI CCCGC 4 cut(s) 318, 574, 2136, 2284
FbaI TGATCA 2 cut(s) 913, 2296
FblI GTMKAC 2 cut(s) 522, 1328
Fnu4HI GCNGC 4 cut(s) 771, 1816, 1887, 2379
FokI GGATG 7 cut(s) 342, 849, 958, 1306, 1746, 1848, 1955
FriOI GRGCYC 2 cut(s) 2349, 2395
Fsp4HI GCNGC 4 cut(s) 771, 1816, 1887, 2379
FspBI CTAG 6 cut(s) 626, 861, 1239, 1482, 1952, 2207
GlaI GCGC 2 cut(s) 485, 1121
GluI GCNGC 4 cut(s) 771, 1816, 1887, 2379
GsaI CCCAGC 2 cut(s) 1665, 2056
GsuI CTGGAG 2 cut(s) 948, 1521
HaeIII GGCC 6 cut(s) 76, 608, 624, 689, 1014, 1893
HapII CCGG 4 cut(s) 882, 1011, 1253, 2402
HhaI GCGC 2 cut(s) 486, 1122
Hin6I GCGC 2 cut(s) 484, 1120
HinP1I GCGC 2 cut(s) 484, 1120
HindIII AAGCTT 4 cut(s) 30, 94, 386, 2231
HpaII CCGG 4 cut(s) 882, 1011, 1253, 2402
HphI GGTGA 5 cut(s) 347, 1055, 2123, 2306, 2351
Hpy166II GTNNAC 9 cut(s) 523, 1244, 1264, 1329, 1337, 1715, 1949, 2080, 2167
Hpy8I GTNNAC 9 cut(s) 523, 1244, 1264, 1329, 1337, 1715, 1949, 2080, 2167
Hpy99I CGWCG 1 cut(s) 2257
HpyAV CCTTC 8 cut(s) 65, 178, 185, 646, 731, 1393, 1614, 2071
HpyCH4III ACNGT 6 cut(s) 527, 1427, 1608, 1799, 1844, 2199
HpyCH4IV ACGT 1 cut(s) 1711
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 109, 124, 1055
HpyF3I CTNAG 7 cut(s) 216, 775, 926, 1194, 1310, 1365, 2412
HpySE526I ACGT 1 cut(s) 1711
HspAI GCGC 2 cut(s) 484, 1120
KpnI GGTACC 1 cut(s) 1011
Ksp22I TGATCA 2 cut(s) 913, 2296
Kzo9I GATC 8 cut(s) 589, 913, 968, 1350, 1520, 1573, 2158, 2296
LguI GCTCTTC 2 cut(s) 1805, 2354
LmnI GCTCC 8 cut(s) 100, 264, 616, 967, 1502, 2057, 2323, 2344
Lsp1109I GCAGC 4 cut(s) 757, 1802, 1873, 2390
LweI GCATC 8 cut(s) 9, 99, 1086, 1165, 1204, 1724, 2200, 2323
MaeI CTAG 6 cut(s) 626, 861, 1239, 1482, 1952, 2207
MaeII ACGT 1 cut(s) 1711
MaeIII GTNAC 8 cut(s) 920, 940, 1228, 1291, 1484, 1608, 1838, 1901
MalI GATC 8 cut(s) 591, 915, 970, 1352, 1522, 1575, 2160, 2298
MbiI CCGCTC 1 cut(s) 256
MboI GATC 8 cut(s) 589, 913, 968, 1350, 1520, 1573, 2158, 2296
MflI RGATCY 2 cut(s) 1350, 1520
MhlI GDGCHC 5 cut(s) 964, 1668, 1877, 2349, 2395
MlyI GAGTC 3 cut(s) 32, 769, 1861
MmeI TCCRAC 3 cut(s) 60, 70, 1510
Mph1103I ATGCAT 1 cut(s) 1494
MroXI GAANNNNTTC 1 cut(s) 1071
MseI TTAA 7 cut(s) 270, 765, 851, 978, 1641, 2066, 2235
MslI CAYNNNNRTG 4 cut(s) 1475, 1732, 2331, 2386
MspCI CTTAAG 1 cut(s) 1640
MspI CCGG 4 cut(s) 882, 1011, 1253, 2402
MspR9I CCNGG 7 cut(s) 686, 966, 1041, 1184, 1253, 2030, 2437
Mva1269I GAATGC 1 cut(s) 244
MvaI CCWGG 6 cut(s) 686, 966, 1041, 1184, 2030, 2437
MvnI CGCG 1 cut(s) 2291
MwoI GCNNNNNNNGC 4 cut(s) 93, 109, 124, 1055
NciI CCSGG 1 cut(s) 1253
NdeII GATC 8 cut(s) 589, 913, 968, 1350, 1520, 1573, 2158, 2296
NlaIV GGNNCC 5 cut(s) 716, 1009, 1667, 1780, 2059
NmeAIII GCCGAG 2 cut(s) 102, 1009
NmuCI GTSAC 2 cut(s) 1838, 1901
NsiI ATGCAT 1 cut(s) 1494
NspI RCATGY 2 cut(s) 1335, 1492
NspV TTCGAA 2 cut(s) 203, 1808
PagI TCATGA 1 cut(s) 886
PciI ACATGT 1 cut(s) 1331
PciSI GCTCTTC 2 cut(s) 1805, 2354
PcsI WCGNNNNNNNCGW 1 cut(s) 1612
PctI GAATGC 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 7 cut(s) 309, 355, 779, 1706, 2023, 2257, 2423
PfoI TCCNGGA 1 cut(s) 964
PkrI GCNGC 4 cut(s) 772, 1817, 1888, 2380
PleI GAGTC 3 cut(s) 32, 768, 1860
PpsI GAGTC 3 cut(s) 32, 768, 1860
Ppu21I YACGTR 1 cut(s) 1712
PscI ACATGT 1 cut(s) 1331
PshBI ATTAAT 1 cut(s) 978
PsiI TTATAA 1 cut(s) 696
Psp124BI GAGCTC 1 cut(s) 2349
Psp6I CCWGG 6 cut(s) 684, 964, 1039, 1182, 2028, 2435
PspFI CCCAGC 2 cut(s) 1661, 2052
PspGI CCWGG 6 cut(s) 684, 964, 1039, 1182, 2028, 2435
PspN4I GGNNCC 5 cut(s) 716, 1009, 1667, 1780, 2059
PspPI GGNCC 4 cut(s) 74, 688, 879, 1532
PstI CTGCAG 2 cut(s) 308, 1410
PstNI CAGNNNCTG 1 cut(s) 2384
PsuI RGATCY 2 cut(s) 1350, 1520
RsaI GTAC 3 cut(s) 382, 1009, 1170
RsaNI GTAC 3 cut(s) 381, 1008, 1169
RseI CAYNNNNRTG 4 cut(s) 1475, 1732, 2331, 2386
SacI GAGCTC 1 cut(s) 2349
SapI GCTCTTC 2 cut(s) 1805, 2354
SaqAI TTAA 7 cut(s) 270, 765, 851, 978, 1641, 2066, 2235
SatI GCNGC 4 cut(s) 771, 1816, 1887, 2379
Sau3AI GATC 8 cut(s) 589, 913, 968, 1350, 1520, 1573, 2158, 2296
Sau96I GGNCC 4 cut(s) 74, 688, 879, 1532
SchI GAGTC 3 cut(s) 32, 769, 1861
ScrFI CCNGG 7 cut(s) 686, 966, 1041, 1184, 1253, 2030, 2437
SduI GDGCHC 5 cut(s) 964, 1668, 1877, 2349, 2395
SfaNI GCATC 8 cut(s) 9, 99, 1086, 1165, 1204, 1724, 2200, 2323
SfcI CTRYAG 5 cut(s) 304, 523, 1164, 1354, 1406
SfuI TTCGAA 2 cut(s) 203, 1808
SinI GGWCC 2 cut(s) 879, 1532
SmiMI CAYNNNNRTG 4 cut(s) 1475, 1732, 2331, 2386
SmlI CTYRAG 3 cut(s) 119, 1459, 1640
SmoI CTYRAG 3 cut(s) 119, 1459, 1640
SpeI ACTAGT 1 cut(s) 1238
SsiI CCGC 7 cut(s) 256, 325, 410, 581, 1749, 2143, 2291
SspMI CTAG 6 cut(s) 626, 861, 1239, 1482, 1952, 2207
SstI GAGCTC 1 cut(s) 2349
StyD4I CCNGG 7 cut(s) 684, 964, 1039, 1182, 1251, 2028, 2435
StyI CCWWGG 2 cut(s) 625, 2319
TaaI ACNGT 6 cut(s) 527, 1427, 1608, 1799, 1844, 2199
TaiI ACGT 1 cut(s) 1714
TaqI TCGA 6 cut(s) 203, 592, 1808, 2184, 2255, 2302
TfiI GAWTC 7 cut(s) 309, 355, 779, 1706, 2023, 2257, 2423
Tru1I TTAA 7 cut(s) 270, 765, 851, 978, 1641, 2066, 2235
Tru9I TTAA 7 cut(s) 270, 765, 851, 978, 1641, 2066, 2235
TscAI CASTG 9 cut(s) 319, 514, 532, 538, 916, 1383, 1847, 1877, 1988
TseFI GTSAC 2 cut(s) 1838, 1901
TseI GCWGC 4 cut(s) 770, 1815, 1886, 2378
Tsp45I GTSAC 2 cut(s) 1838, 1901
TspGWI ACGGA 1 cut(s) 1648
TspRI CASTG 9 cut(s) 319, 514, 532, 538, 916, 1383, 1847, 1877, 1988
Vha464I CTTAAG 1 cut(s) 1640
VpaK11BI GGWCC 2 cut(s) 879, 1532
VspI ATTAAT 1 cut(s) 978
XagI CCTNNNNNAGG 1 cut(s) 629
XapI RAATTY 6 cut(s) 59, 129, 232, 572, 594, 2237
XceI RCATGY 2 cut(s) 1335, 1492
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XmaJI CCTAGG 1 cut(s) 625
XmiI GTMKAC 2 cut(s) 522, 1328
XmnI GAANNNNTTC 1 cut(s) 1071
XspI CTAG 6 cut(s) 626, 861, 1239, 1482, 1952, 2207
Zsp2I ATGCAT 1 cut(s) 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.