pycom12397g00230

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012397
OG0019581
Physical Location & Seq
Reverse (-)
119938 .. 121513
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1065 bp
ATGTCTTTCTATGGGATCGCAGGTCTTTTTATTAGCTCCTATTTGTGGTGCACAATTTCGTGGAATGTAGGTAGTGGTTATGATCGATTCGATATAAAAGAGGGCATAGTGTGTATTTTTCGTTGGGGATTTCCTGGAAAAAATCGTCGCATATTCCTCCGATTCCTTATGAAAGACATTCAGTCCATCAGAATAGAAATTAAAGAGGGTATTTATGCTCGTCGTGTCCTTTATATGGAAATAAAAGGACAAGGAGCCATTCCCTTGACTCGTACTGATGAGAATTTTACTCCACGAGAGATTGAGCAAAAAGCTGCTGAATTGTCGACGAATGAGGTGCGTTCATTAAAAATTTTAAAATTCACAGACGAAAAAATGGCAAAAAAGAAAGTATTAATTTCCCTTCTATATCTTGCATCTATAGTATTTTTGCCTTGGTGGATCTCTCTCTCATTTAATAAAAGTCTAGAATCTTGGTTTACTAATTGGTGGAATACTAGGCAATCCGAAATTTTTTTGAATGATATTCAAGAAAAGAGTATTCTAAAAGAATTCATAGACTTAGAGGAACTCTTACGTTTGGACGAAATGATAAAGGAATACCCGGAAACACATTTACAAAAGCCTCGCATCGAAATCTACAAAGAAACGATCCAATTGATCAAGATGCACAACGAGGATCGCATCCATACAATTTTACACTTCTCGACAAATATAATCTGTTTCGGTATTCTAAGTGGTTATTCTATTCTAGGTAATGAAGAACTTGTTATTCTTAACTCTTGGTTTCAAGAATTCCTATACAACTTAAGCGACACAATAAAAGCTTTTTCTATTCTTTTATTAACGGATTTATGTATAGGATTCCATTCGCCCCACGGTTGGGAATTAATGATTGGCTCTGTCTACAAAGATTTTGGATTTGCTCATAATGATCAAATTATATCCGGTCTTGTTTCTACTTTTCCAGTCATTTTAGATACAATTTTTAAATATTGGATCTTTCGTTATTTAAATCGTGTATCTCCTTCACTTGTAGTGATTTATCATTCAATGAATGACTGA

Protein Analysis

355

Amino Acids

41.72

Weight (kDa)

6.47

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf4 PF02392 1 - 109 3.4e-46 Ycf4
CemA PF03040 128 - 354 6.8e-82 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019581)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00530
fragaria_vesca FvH4_3g27991
malus_domestica MD08G1223200.v1.1
prunus_persica Prupe.7G029500_v2.0.a1
pyrus_communis pycom04g07520 pycom12397g00230
rosa_chinensis RchiOBHm_CPg0502081

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 11
AccI GTMKAC 2 cut(s) 326, 906
AclWI GGATC 5 cut(s) 23, 449, 646, 687, 1007
AcsI RAATTY 6 cut(s) 283, 351, 359, 510, 551, 794
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 4 cut(s) 45, 235, 882, 883
AflII CTTAAG 1 cut(s) 808
AgsI TTSAA 4 cut(s) 520, 530, 791, 1053
AhdI GACNNNNNGTC 1 cut(s) 181
AjnI CCWGG 1 cut(s) 133
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AluBI AGCT 3 cut(s) 36, 314, 827
AluI AGCT 3 cut(s) 36, 314, 827
Alw21I GWGCWC 1 cut(s) 53
Alw44I GTGCAC 1 cut(s) 49
AlwI GGATC 5 cut(s) 23, 449, 646, 687, 1007
ApaLI GTGCAC 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 314
ApoI RAATTY 6 cut(s) 283, 351, 359, 510, 551, 794
AseI ATTAAT 2 cut(s) 395, 890
AsuC2I CCSGG 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 53
BauI CACGAG 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 301
BccI CCATC 1 cut(s) 194
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciT130I CCWGG 1 cut(s) 135
BclI TGATCA 2 cut(s) 660, 934
BcnI CCSGG 1 cut(s) 605
BfaI CTAG 3 cut(s) 467, 498, 752
BfmI CTRYAG 1 cut(s) 420
BfrI CTTAAG 1 cut(s) 808
BfuAI ACCTGC 1 cut(s) 11
BisI GCNGC 1 cut(s) 315
BlsI GCNGC 1 cut(s) 316
Bme1390I CCNGG 2 cut(s) 135, 605
BmeRI GACNNNNNGTC 1 cut(s) 181
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 2 cut(s) 135, 605
BmsI GCATC 4 cut(s) 425, 639, 657, 693
BpuMI CCSGG 1 cut(s) 605
Bsa29I ATCGAT 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 434, 877
BsaWI WCCGGW 1 cut(s) 947
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 235, 882, 883
Bse1I ACTGG 1 cut(s) 968
BseBI CCWGG 1 cut(s) 135
BseCI ATCGAT 1 cut(s) 85
BseDI CCNNGG 2 cut(s) 434, 877
BseGI GGATG 1 cut(s) 684
BseLI CCNNNNNNNGG 4 cut(s) 45, 235, 882, 883
BseNI ACTGG 1 cut(s) 968
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 1 cut(s) 301
BshVI ATCGAT 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 2 cut(s) 605, 948
BslI CCNNNNNNNGG 4 cut(s) 45, 235, 882, 883
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 8 cut(s) 15, 82, 441, 651, 660, 679, 934, 999
BspDI ATCGAT 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 256
BspMI ACCTGC 1 cut(s) 11
BspPI GGATC 5 cut(s) 23, 449, 646, 687, 1007
BspTI CTTAAG 1 cut(s) 808
BsrI ACTGG 1 cut(s) 968
BssECI CCNNGG 2 cut(s) 434, 877
BssMI GATC 8 cut(s) 15, 82, 441, 651, 660, 679, 934, 999
BssSI CACGAG 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 434
Bst2BI CACGAG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 881
BstAFI CTTAAG 1 cut(s) 808
BstDEI CTNAG 2 cut(s) 562, 734
BstDSI CCRYGG 1 cut(s) 877
BstF5I GGATG 1 cut(s) 684
BstKTI GATC 8 cut(s) 18, 85, 444, 654, 663, 682, 937, 1002
BstMBI GATC 8 cut(s) 15, 82, 441, 651, 660, 679, 934, 999
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 2 cut(s) 133, 603
BstSFI CTRYAG 1 cut(s) 420
BstSLI GKGCMC 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 301
BstX2I RGATCY 2 cut(s) 441, 999
BstYI RGATCY 2 cut(s) 441, 999
Bsu15I ATCGAT 1 cut(s) 85
BsuTUI ATCGAT 1 cut(s) 85
BtgI CCRYGG 1 cut(s) 877
BtsCI GGATG 1 cut(s) 684
BveI ACCTGC 1 cut(s) 11
ClaI ATCGAT 1 cut(s) 85
Csp6I GTAC 1 cut(s) 273
CviJI RGCY 6 cut(s) 36, 257, 314, 625, 827, 900
CviKI_1 RGCY 6 cut(s) 36, 257, 314, 625, 827, 900
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 2 cut(s) 562, 734
DpnI GATC 8 cut(s) 17, 84, 443, 653, 662, 681, 936, 1001
DpnII GATC 8 cut(s) 15, 82, 441, 651, 660, 679, 934, 999
DraI TTTAAA 3 cut(s) 357, 991, 1014
DriI GACNNNNNGTC 1 cut(s) 181
Eam1105I GACNNNNNGTC 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 434
EcoRI GAATTC 2 cut(s) 551, 794
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 434
FbaI TGATCA 2 cut(s) 660, 934
FblI GTMKAC 2 cut(s) 326, 906
Fnu4HI GCNGC 1 cut(s) 315
FokI GGATG 1 cut(s) 671
Fsp4HI GCNGC 1 cut(s) 315
FspBI CTAG 3 cut(s) 467, 498, 752
GluI GCNGC 1 cut(s) 315
HapII CCGG 2 cut(s) 605, 948
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HindIII AAGCTT 1 cut(s) 825
HinfI GANTC 5 cut(s) 87, 162, 268, 470, 864
HpaII CCGG 2 cut(s) 605, 948
Hpy166II GTNNAC 4 cut(s) 51, 327, 480, 907
Hpy188I TCNGA 3 cut(s) 161, 191, 508
Hpy188III TCNNGA 5 cut(s) 467, 530, 664, 706, 791
Hpy8I GTNNAC 4 cut(s) 51, 327, 480, 907
Hpy99I CGWCG 3 cut(s) 150, 225, 331
HpyAV CCTTC 2 cut(s) 413, 1038
HpyCH4III ACNGT 1 cut(s) 881
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 3 cut(s) 51, 416, 670
HpyF3I CTNAG 2 cut(s) 562, 734
HpySE526I ACGT 1 cut(s) 577
Ksp22I TGATCA 2 cut(s) 660, 934
Kzo9I GATC 8 cut(s) 15, 82, 441, 651, 660, 679, 934, 999
LmnI GCTCC 2 cut(s) 41, 254
LpnPI CCDG 6 cut(s) 6, 120, 147, 618, 961, 981
Lsp1109I GCAGC 1 cut(s) 301
LweI GCATC 4 cut(s) 425, 639, 657, 693
MaeI CTAG 3 cut(s) 467, 498, 752
MaeII ACGT 1 cut(s) 577
MalI GATC 8 cut(s) 17, 84, 443, 653, 662, 681, 936, 1001
MboI GATC 8 cut(s) 15, 82, 441, 651, 660, 679, 934, 999
MboII GAAGA 1 cut(s) 773
MfeI CAATTG 1 cut(s) 656
MflI RGATCY 2 cut(s) 441, 999
MhlI GDGCHC 1 cut(s) 53
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 7 cut(s) 94, 167, 199, 328, 559, 636, 670
MspCI CTTAAG 1 cut(s) 808
MspI CCGG 2 cut(s) 605, 948
MspR9I CCNGG 2 cut(s) 135, 605
MunI CAATTG 1 cut(s) 656
MvaI CCWGG 1 cut(s) 135
NciI CCSGG 1 cut(s) 605
NdeII GATC 8 cut(s) 15, 82, 441, 651, 660, 679, 934, 999
NlaIV GGNNCC 1 cut(s) 256
PfeI GAWTC 4 cut(s) 87, 162, 470, 864
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 1 cut(s) 316
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
PshBI ATTAAT 2 cut(s) 395, 890
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 256
PsuI RGATCY 2 cut(s) 441, 999
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
SalI GTCGAC 1 cut(s) 325
SatI GCNGC 1 cut(s) 315
Sau3AI GATC 8 cut(s) 15, 82, 441, 651, 660, 679, 934, 999
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 2 cut(s) 135, 605
SduI GDGCHC 1 cut(s) 53
SetI ASST 8 cut(s) 25, 38, 73, 316, 339, 580, 757, 829
SfaNI GCATC 4 cut(s) 425, 639, 657, 693
SfcI CTRYAG 1 cut(s) 420
SmiI ATTTAAAT 1 cut(s) 1014
SmlI CTYRAG 1 cut(s) 808
SmoI CTYRAG 1 cut(s) 808
SspI AATATT 1 cut(s) 995
SspMI CTAG 3 cut(s) 467, 498, 752
StyD4I CCNGG 2 cut(s) 133, 603
StyI CCWWGG 1 cut(s) 434
SwaI ATTTAAAT 1 cut(s) 1014
TaaI ACNGT 1 cut(s) 881
TaiI ACGT 1 cut(s) 580
TaqI TCGA 5 cut(s) 85, 90, 326, 633, 707
TfiI GAWTC 4 cut(s) 87, 162, 470, 864
TseI GCWGC 1 cut(s) 314
TspDTI ATGAA 4 cut(s) 185, 333, 544, 774
TspGWI ACGGA 1 cut(s) 863
Vha464I CTTAAG 1 cut(s) 808
VneI GTGCAC 1 cut(s) 49
VspI ATTAAT 2 cut(s) 395, 890
XapI RAATTY 6 cut(s) 283, 351, 359, 510, 551, 794
XbaI TCTAGA 1 cut(s) 466
XmiI GTMKAC 2 cut(s) 326, 906
XspI CTAG 3 cut(s) 467, 498, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.