pycom12397g00350

ATP synthase subunit alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012397
OG0009873
Physical Location & Seq
Forward (+)
172733 .. 174256
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12397g00350.1

Sequence Viewer

Length: 1524 bp
ATGGTAACCATTCGAGCAGATGAAATTAGTAATATTATCCGCGAGCGTATTGAGCAATATAATAGAGAAGTAAAGATTTTAAATACCGGTACTGTACTTCAAGTAGGCGATGGTATTGCTCGTATTTATGGTCTTGATGAAGTAATGGCAGGTGAATTAGTAGAATTTGAAGAGGGTACCATAGGCATTGCTCTGAATTTGGAATCAAATAATGTTGGTGTTGTATTAATGGGTGACGGTTTGATGATACAAGAGGGGAGTTCTGTAAAAGCAACAGGAAGAATTGCTCAGATACCAGTAAGTGAGGCTTTTTTAGGTCGTGTTATAAATGCACTGGCTAAACCTATTGATGGTCGGGGTGAAATTTCATCTTCTGAATCTCGATTAATTGAGTCTCCCGCTCCTGGTATTATTTCGAGACGTTCCGTATACGAGCCTCTTCAAACAGGACTTATTGCTATTGATTCGATGATTCCTATAGGGCGTGGTCAGCGAGAATTAATTATTGGGGACAGACAGACCGGTAAAACAGCAGTAGCCACAGATACTATTCTCAATCAACAAGGGCAAAATGTAATATGCGTTTATGTAGCTATTGGTCAAAAAGCATCTTCTGTGGCTCAGGTGGTGACTGCTTTACAGGAAAGGGGAGCAATGGAATACACTATTGTGGTAGCTGAAACTGCGGACTCCCCCGCTACATTACAATACCTTGCTCCTTATACAGGAGCAGCTCTGGCTGAATATTTTATGTACCGTGAACAACACACTTCAATCATTTATGACGATCTCTCCAAACAAGCGCAGGCGTATCGACAAATGTCTCTTCTATTACGAAGACCACCGGGCCGCGAAGCTTATCCGGGAGATGTTTTTTATTTACATTCACGCCTTTTGGAAAGAGCCGCTAAATCAAGTTCTCGTTTAGGTGAAGGAAGTATGACTGCTTTACCAATAGTTGAGACCCAATCGGGAGATGTTTCGGCTTATATTCCTACTAATGTAATTTCCATTACAGATGGACAAATATTCTTATCTGCCGATTTATTCAATTCTGGAATCAGACCGGCCATTAATGTGGGTATTTCGGTTTCCAGGGTAGGATCCGCAGCTCAAATTAAAGCCATGAAACAAGTAGCTGGCAAATCAAAATTGGAATTGGCACAATTTGCAGAATTAGAAGCCTTTGCACAATTCGCTTCTGATCTCGATAAAGCTACTCAGAATCAATTGGCAAGAGGTCAACGATTACGTGAGTTGCTTAAACAATCTCAATCATCCCCGCTTTCGGTGGAAGAACAGATACTGACTATTTATACCGGAACGAACGGTTATCTTGATTCATTAGAAATTGGACAGGTAAGGAAATTTCTTGTTGAGTTACGTACTTACTTAAAAACGAATAAACCTCAGTTTCAAGAAATAATATCTTCTACCAAGACATTTACCGACGAAGCGGAAGCCCTTTTGAAAGAAGCTATTCAGGAACAGATGGAACGTTTTCTACTTCAGGAACAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko00195 ko01100 map00190 map00195 map01100
Pfam Domains
Protein Families
ATP-synt_C ATP-synt_ab ATP-synt_ab_C ATP-synt_ab_N

Protein Analysis

508

Amino Acids

55.42

Weight (kDa)

5.09

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 29 - 93 7.4e-18 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab PF00006 150 - 365 1.1e-82 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_ab_C PF00306 372 - 496 4.9e-44 ATP synthase alpha/beta chain, C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AarI CACCTGC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 140
Acc65I GGTACC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 401
AccI GTMKAC 1 cut(s) 429
AccII CGCG 2 cut(s) 42, 852
AciI CCGC 9 cut(s) 40, 399, 686, 696, 850, 906, 1107, 1283, 1457
AclI AACGTT 1 cut(s) 1498
AclWI GGATC 2 cut(s) 1098, 1111
AcoI YGGCCR 1 cut(s) 1068
AcsI RAATTY 4 cut(s) 164, 196, 363, 1367
AcuI CTGAAG 1 cut(s) 1493
AfaI GTAC 5 cut(s) 91, 96, 178, 755, 1387
AfiI CCNNNNNNNGG 4 cut(s) 350, 404, 725, 1288
AgeI ACCGGT 2 cut(s) 86, 521
AgsI TTSAA 7 cut(s) 101, 170, 443, 774, 1051, 1418, 1471
AjnI CCWGG 2 cut(s) 403, 1094
AjuI GAANNNNNNNTTGG 2 cut(s) 489, 521
AleI CACNNNNGTG 1 cut(s) 668
AluBI AGCT 8 cut(s) 593, 677, 734, 857, 1112, 1139, 1217, 1478
AluI AGCT 8 cut(s) 593, 677, 734, 857, 1112, 1139, 1217, 1478
Alw26I GTCTC 4 cut(s) 399, 412, 828, 956
AlwI GGATC 2 cut(s) 1098, 1111
AlwNI CAGNNNCTG 1 cut(s) 1306
AoxI GGCC 2 cut(s) 847, 1068
ApeKI GCWGC 2 cut(s) 731, 1109
ApoI RAATTY 4 cut(s) 164, 196, 363, 1367
AseI ATTAAT 4 cut(s) 227, 386, 500, 1074
AsiGI ACCGGT 2 cut(s) 86, 521
Asp700I GAANNNNTTC 2 cut(s) 1479, 1500
Asp718I GGTACC 1 cut(s) 176
AspLEI GCGC 1 cut(s) 805
AspS9I GGNCC 1 cut(s) 847
AsuC2I CCSGG 2 cut(s) 846, 864
AsuHPI GGTGA 5 cut(s) 164, 245, 371, 640, 941
BamHI GGATCC 1 cut(s) 1103
BanI GGYRCC 1 cut(s) 176
BarI GAAGNNNNNNTAC 2 cut(s) 1287, 1319
BbsI GAAGAC 1 cut(s) 844
BbvI GCAGC 2 cut(s) 743, 1121
BccI CCATC 4 cut(s) 104, 344, 1013, 1486
BcgI CGANNNNNNTGC 4 cut(s) 98, 132, 794, 828
BciT130I CCWGG 2 cut(s) 405, 1096
BcnI CCSGG 2 cut(s) 846, 864
BcoDI GTCTC 4 cut(s) 399, 412, 828, 956
BfmI CTRYAG 1 cut(s) 477
BfuAI ACCTGC 1 cut(s) 140
BisI GCNGC 4 cut(s) 732, 850, 906, 1110
BlsI GCNGC 4 cut(s) 733, 851, 907, 1111
Bme1390I CCNGG 4 cut(s) 405, 846, 864, 1096
BmgT120I GGNCC 1 cut(s) 847
BmiI GGNNCC 2 cut(s) 178, 1105
BmrFI CCNGG 4 cut(s) 405, 846, 864, 1096
BmsI GCATC 1 cut(s) 617
BoxI GACNNNNGTC 1 cut(s) 820
BpiI GAAGAC 1 cut(s) 844
Bpu10I CCTNAGC 1 cut(s) 621
BpuMI CCSGG 2 cut(s) 846, 864
BsaAI YACGTR 2 cut(s) 1253, 1385
BsaI GGTCTC 1 cut(s) 956
BsaJI CCNNGG 1 cut(s) 1095
BsaWI WCCGGW 3 cut(s) 86, 521, 1319
BsaXI ACNNNNNCTCC 2 cut(s) 776, 806
Bsc4I CCNNNNNNNGG 4 cut(s) 350, 404, 725, 1288
Bse118I RCCGGY 3 cut(s) 86, 521, 1066
Bse1I ACTGG 2 cut(s) 296, 339
Bse3DI GCAATG 2 cut(s) 186, 660
BseBI CCWGG 2 cut(s) 405, 1096
BseDI CCNNGG 1 cut(s) 1095
BseGI GGATG 1 cut(s) 1277
BseLI CCNNNNNNNGG 4 cut(s) 350, 404, 725, 1288
BseMI GCAATG 2 cut(s) 186, 660
BseMII CTCAG 4 cut(s) 302, 635, 1235, 1424
BseNI ACTGG 2 cut(s) 296, 339
BseXI GCAGC 2 cut(s) 743, 1121
Bsh1236I CGCG 2 cut(s) 42, 852
BshFI GGCC 2 cut(s) 849, 1070
BshNI GGYRCC 1 cut(s) 176
BshTI ACCGGT 2 cut(s) 86, 521
BsiSI CCGG 6 cut(s) 87, 522, 845, 863, 1067, 1320
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 4 cut(s) 350, 404, 725, 1288
BsmAI GTCTC 4 cut(s) 399, 412, 828, 956
BsmBI CGTCTC 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 2 cut(s) 849, 1070
Bso31I GGTCTC 1 cut(s) 956
Bsp143I GATC 3 cut(s) 787, 1103, 1204
BspACI CCGC 9 cut(s) 40, 399, 686, 696, 850, 906, 1107, 1283, 1457
BspANI GGCC 2 cut(s) 849, 1070
BspCNI CTCAG 4 cut(s) 301, 634, 1234, 1423
BspFNI CGCG 2 cut(s) 42, 852
BspLI GGNNCC 2 cut(s) 178, 1105
BspMI ACCTGC 1 cut(s) 140
BspPI GGATC 2 cut(s) 1098, 1111
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 956
BsrBI CCGCTC 1 cut(s) 401
BsrDI GCAATG 2 cut(s) 186, 660
BsrFI RCCGGY 3 cut(s) 86, 521, 1066
BsrI ACTGG 2 cut(s) 296, 339
BssAI RCCGGY 3 cut(s) 86, 521, 1066
BssECI CCNNGG 1 cut(s) 1095
BssMI GATC 3 cut(s) 787, 1103, 1204
BssNAI GTATAC 1 cut(s) 430
Bst1107I GTATAC 1 cut(s) 430
Bst2UI CCWGG 2 cut(s) 405, 1096
Bst4CI ACNGT 4 cut(s) 94, 239, 758, 1331
Bst6I CTCTTC 3 cut(s) 165, 444, 831
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstBAI YACGTR 2 cut(s) 1253, 1385
BstC8I GCNNGC 3 cut(s) 44, 807, 1141
BstDEI CTNAG 4 cut(s) 288, 621, 1221, 1410
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 1 cut(s) 723
BstF5I GGATG 1 cut(s) 1277
BstFNI CGCG 2 cut(s) 42, 852
BstHHI GCGC 1 cut(s) 805
BstKTI GATC 3 cut(s) 790, 1106, 1207
BstMAI GTCTC 4 cut(s) 399, 412, 828, 956
BstMBI GATC 3 cut(s) 787, 1103, 1204
BstMWI GCNNNNNNNGC 6 cut(s) 52, 490, 683, 737, 1169, 1196
BstNI CCWGG 2 cut(s) 405, 1096
BstPAI GACNNNNGTC 1 cut(s) 820
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 4 cut(s) 403, 844, 862, 1094
BstSFI CTRYAG 1 cut(s) 477
BstSNI TACGTA 1 cut(s) 1385
BstUI CGCG 2 cut(s) 42, 852
BstV1I GCAGC 2 cut(s) 743, 1121
BstV2I GAAGAC 1 cut(s) 844
BstX2I RGATCY 1 cut(s) 1103
BstXI CCANNNNNNTGG 1 cut(s) 1078
BstYI RGATCY 1 cut(s) 1103
BstZ17I GTATAC 1 cut(s) 430
BsuRI GGCC 2 cut(s) 849, 1070
BtgZI GCGATG 1 cut(s) 123
BtsCI GGATG 1 cut(s) 1277
BtsIMutI CAGTG 1 cut(s) 332
BveI ACCTGC 1 cut(s) 140
Cac8I GCNNGC 3 cut(s) 44, 807, 1141
CaiI CAGNNNCTG 1 cut(s) 1306
CfoI GCGC 1 cut(s) 805
Cfr10I RCCGGY 3 cut(s) 86, 521, 1066
Cfr13I GGNCC 1 cut(s) 847
Csp6I GTAC 5 cut(s) 90, 95, 177, 754, 1386
CspAI ACCGGT 2 cut(s) 86, 521
CviAII CATG 1 cut(s) 1126
CviQI GTAC 5 cut(s) 90, 95, 177, 754, 1386
DdeI CTNAG 4 cut(s) 288, 621, 1221, 1410
DpnI GATC 3 cut(s) 789, 1105, 1206
DpnII GATC 3 cut(s) 787, 1103, 1204
DraI TTTAAA 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 1068
Eam1104I CTCTTC 3 cut(s) 165, 444, 831
EarI CTCTTC 3 cut(s) 165, 444, 831
Eco105I TACGTA 1 cut(s) 1385
Eco31I GGTCTC 1 cut(s) 956
Eco57I CTGAAG 1 cut(s) 1493
Eco91I GGTNACC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 723
EcoO65I GGTNACC 1 cut(s) 4
EcoRII CCWGG 2 cut(s) 403, 1094
Esp3I CGTCTC 1 cut(s) 412
FaeI CATG 1 cut(s) 1129
FalI AAGNNNNNCTT 2 cut(s) 292, 324
FaqI GGGAC 1 cut(s) 524
FatI CATG 1 cut(s) 1125
FauI CCCGC 3 cut(s) 406, 703, 1290
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 4 cut(s) 732, 850, 906, 1110
FokI GGATG 1 cut(s) 1264
Fsp4HI GCNGC 4 cut(s) 732, 850, 906, 1110
GlaI GCGC 1 cut(s) 804
GluI GCNGC 4 cut(s) 732, 850, 906, 1110
HaeIII GGCC 2 cut(s) 849, 1070
HapII CCGG 6 cut(s) 87, 522, 845, 863, 1067, 1320
HhaI GCGC 1 cut(s) 805
Hin1II CATG 1 cut(s) 1129
Hin6I GCGC 1 cut(s) 803
HinP1I GCGC 1 cut(s) 803
HincII GTYRAC 1 cut(s) 1244
HindII GTYRAC 1 cut(s) 1244
HindIII AAGCTT 1 cut(s) 855
HinfI GANTC 9 cut(s) 203, 377, 392, 464, 472, 689, 1059, 1225, 1340
HpaII CCGG 6 cut(s) 87, 522, 845, 863, 1067, 1320
HphI GGTGA 5 cut(s) 164, 245, 371, 640, 941
Hpy166II GTNNAC 3 cut(s) 430, 761, 1244
Hpy188I TCNGA 6 cut(s) 195, 291, 376, 1064, 1204, 1224
Hpy8I GTNNAC 3 cut(s) 430, 761, 1244
Hpy99I CGWCG 1 cut(s) 1454
HpyAV CCTTC 1 cut(s) 926
HpyCH4III ACNGT 4 cut(s) 94, 239, 758, 1331
HpyCH4IV ACGT 4 cut(s) 421, 1252, 1384, 1498
HpyCH4V TGCA 3 cut(s) 332, 1172, 1190
HpyF10VI GCNNNNNNNGC 6 cut(s) 52, 490, 683, 737, 1169, 1196
HpyF3I CTNAG 4 cut(s) 288, 621, 1221, 1410
HpySE526I ACGT 4 cut(s) 421, 1252, 1384, 1498
Hsp92II CATG 1 cut(s) 1129
HspAI GCGC 1 cut(s) 803
KpnI GGTACC 1 cut(s) 180
Kzo9I GATC 3 cut(s) 787, 1103, 1204
LmnI GCTCC 4 cut(s) 406, 650, 721, 728
Lsp1109I GCAGC 2 cut(s) 743, 1121
LweI GCATC 1 cut(s) 617
MaeII ACGT 4 cut(s) 421, 1252, 1384, 1498
MaeIII GTNAC 4 cut(s) 4, 233, 628, 1380
MalI GATC 3 cut(s) 789, 1105, 1206
MbiI CCGCTC 1 cut(s) 401
MboI GATC 3 cut(s) 787, 1103, 1204
MboII GAAGA 9 cut(s) 182, 291, 363, 431, 603, 818, 849, 1307, 1422
MfeI CAATTG 1 cut(s) 1229
MflI RGATCY 1 cut(s) 1103
MlyI GAGTC 2 cut(s) 401, 683
MnlI CCTC 6 cut(s) 166, 247, 298, 447, 1232, 1419
MroXI GAANNNNTTC 2 cut(s) 1479, 1500
MseI TTAA 8 cut(s) 80, 227, 386, 500, 1074, 1119, 1263, 1394
MslI CAYNNNNRTG 2 cut(s) 668, 1076
MspI CCGG 6 cut(s) 87, 522, 845, 863, 1067, 1320
MspR9I CCNGG 4 cut(s) 405, 846, 864, 1096
MunI CAATTG 1 cut(s) 1229
MvaI CCWGG 2 cut(s) 405, 1096
MvnI CGCG 2 cut(s) 42, 852
MwoI GCNNNNNNNGC 6 cut(s) 52, 490, 683, 737, 1169, 1196
NciI CCSGG 2 cut(s) 846, 864
NdeII GATC 3 cut(s) 787, 1103, 1204
NlaIII CATG 1 cut(s) 1129
NlaIV GGNNCC 2 cut(s) 178, 1105
NmuCI GTSAC 2 cut(s) 233, 628
OliI CACNNNNGTG 1 cut(s) 668
PaqCI CACCTGC 1 cut(s) 140
PdmI GAANNNNTTC 2 cut(s) 1479, 1500
PfeI GAWTC 7 cut(s) 203, 377, 464, 472, 1059, 1225, 1340
PfoI TCCNGGA 1 cut(s) 862
PinAI ACCGGT 2 cut(s) 86, 521
PkrI GCNGC 4 cut(s) 733, 851, 907, 1111
PleI GAGTC 2 cut(s) 400, 683
PpsI GAGTC 2 cut(s) 400, 683
Ppu21I YACGTR 2 cut(s) 1253, 1385
PshAI GACNNNNGTC 1 cut(s) 820
PshBI ATTAAT 4 cut(s) 227, 386, 500, 1074
PsiI TTATAA 1 cut(s) 326
Psp1406I AACGTT 1 cut(s) 1498
Psp6I CCWGG 2 cut(s) 403, 1094
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 403, 1094
PspN4I GGNNCC 2 cut(s) 178, 1105
PspPI GGNCC 1 cut(s) 847
PsrI GAACNNNNNNTAC 2 cut(s) 1488, 1520
PstNI CAGNNNCTG 1 cut(s) 1306
PsuI RGATCY 1 cut(s) 1103
RsaI GTAC 5 cut(s) 91, 96, 178, 755, 1387
RsaNI GTAC 5 cut(s) 90, 95, 177, 754, 1386
RseI CAYNNNNRTG 2 cut(s) 668, 1076
SaqAI TTAA 8 cut(s) 80, 227, 386, 500, 1074, 1119, 1263, 1394
SatI GCNGC 4 cut(s) 732, 850, 906, 1110
Sau3AI GATC 3 cut(s) 787, 1103, 1204
Sau96I GGNCC 1 cut(s) 847
SchI GAGTC 2 cut(s) 401, 683
ScrFI CCNGG 4 cut(s) 405, 846, 864, 1096
SfaNI GCATC 1 cut(s) 617
SfcI CTRYAG 1 cut(s) 477
SmiMI CAYNNNNRTG 2 cut(s) 668, 1076
SnaBI TACGTA 1 cut(s) 1385
SsiI CCGC 9 cut(s) 40, 399, 686, 696, 850, 906, 1107, 1283, 1457
SspI AATATT 3 cut(s) 34, 746, 1029
StyD4I CCNGG 4 cut(s) 403, 844, 862, 1094
TaaI ACNGT 4 cut(s) 94, 239, 758, 1331
TaiI ACGT 4 cut(s) 424, 1255, 1387, 1501
TaqI TCGA 6 cut(s) 13, 382, 416, 467, 814, 1209
TatI WGTACW 1 cut(s) 94
TauI GCSGC 2 cut(s) 852, 908
TfiI GAWTC 7 cut(s) 203, 377, 464, 472, 1059, 1225, 1340
Tru1I TTAA 8 cut(s) 80, 227, 386, 500, 1074, 1119, 1263, 1394
Tru9I TTAA 8 cut(s) 80, 227, 386, 500, 1074, 1119, 1263, 1394
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 2 cut(s) 233, 628
TseI GCWGC 2 cut(s) 731, 1109
Tsp45I GTSAC 2 cut(s) 233, 628
TspDTI ATGAA 5 cut(s) 36, 153, 357, 1142, 1332
TspGWI ACGGA 1 cut(s) 415
TspRI CASTG 1 cut(s) 339
VspI ATTAAT 4 cut(s) 227, 386, 500, 1074
XagI CCTNNNNNAGG 1 cut(s) 723
XapI RAATTY 4 cut(s) 164, 196, 363, 1367
XmiI GTMKAC 1 cut(s) 429
XmnI GAANNNNTTC 2 cut(s) 1479, 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.