pycom12g01300

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008046
Physical Location & Seq
Forward (+)
1088028 .. 1091326
3299 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
ATGGGAATGGGAATGGGATTGCTGGGGAGCTCAACAATCCCATTGGGAGTGGGAATGGGAGGAGTAGTGGGCGAGCATTGCTGGGAATGGGGGAGGAAGTACTTGGAGTTCGAAGTCTGCAGCGTGAAGGACGGCATGTCGTTGGCGCTGGGTGTAATCAGCGTACTCAGTTGGGGAGTCGCTGAGGTTCCCCAAATCATCACCAACTACAAGGCCAAATGCAGTCACGGCCTCTCTCTTGCTTTCTTGATCACTTGGATTATCGGGGTTCCAAGACTAATCCGGCTGAGGTTCCAGGAAAAGCTAGTGGCAGGATCGGCAAGATCTTTATCAAGGAGTCACACCCCTTCAGAAGGATTTCATGCAGCACAAAGAACAACTCTTCCATTTAATCGTAATCGAAATCCAAATGAAGAGCCCTTGCTTGGTACACTTGCATCTACACAATCTGCACCGTCTAATAATGTCAAGACTACGCTGTGTGTGGTCTCGCTGATGACCCTCTTTTGCACTTTTAATCAACGATCAGTTGATTTAAGTGTTGATAATCCAAATCGAGGAGTAGTGATGCAAGTAGGAAGAAGGCTGTTGCAGGTAGTTGACATTCCAAAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

206

Amino Acids

22.36

Weight (kDa)

9.46

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 47 - 89 1.4e-12 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 583
AclWI GGATC 1 cut(s) 322
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 3 cut(s) 101, 165, 430
AfiI CCNNNNNNNGG 3 cut(s) 353, 425, 557
AhdI GACNNNNNGTC 1 cut(s) 136
AjnI CCWGG 1 cut(s) 294
AloI GAACNNNNNNTCC 2 cut(s) 367, 399
AluBI AGCT 2 cut(s) 30, 304
AluI AGCT 2 cut(s) 30, 304
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 493
AlwI GGATC 1 cut(s) 322
AoxI GGCC 2 cut(s) 213, 229
ApeKI GCWGC 2 cut(s) 120, 365
Asp700I GAANNNNTTC 1 cut(s) 357
AspLEI GCGC 1 cut(s) 148
AsuHPI GGTGA 1 cut(s) 193
AsuII TTCGAA 1 cut(s) 111
BanII GRGCYC 2 cut(s) 32, 420
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 2 cut(s) 183, 287
BbvI GCAGC 2 cut(s) 132, 377
BceAI ACGGC 2 cut(s) 148, 244
BciT130I CCWGG 1 cut(s) 296
BclI TGATCA 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 1 cut(s) 305
BfmI CTRYAG 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 583
BglII AGATCT 1 cut(s) 323
BisI GCNGC 2 cut(s) 121, 366
BlsI GCNGC 2 cut(s) 122, 367
BmcAI AGTACT 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 296
BmeRI GACNNNNNGTC 1 cut(s) 136
BmiI GGNNCC 3 cut(s) 189, 270, 293
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 2 cut(s) 446, 558
Bpu10I CCTNAGC 2 cut(s) 183, 287
Bpu14I TTCGAA 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 328
BsaI GGTCTC 1 cut(s) 493
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 425, 557
Bse3DI GCAATG 1 cut(s) 76
Bse8I GATNNNNATC 1 cut(s) 328
BseBI CCWGG 1 cut(s) 296
BseJI GATNNNNATC 1 cut(s) 328
BseLI CCNNNNNNNGG 3 cut(s) 353, 425, 557
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 3 cut(s) 174, 181, 278
BseRI GAGGAG 2 cut(s) 75, 573
BseXI GCAGC 2 cut(s) 132, 377
BseYI CCCAGC 3 cut(s) 22, 81, 148
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 2 cut(s) 215, 231
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 283
BslI CCNNNNNNNGG 3 cut(s) 353, 425, 557
BsmAI GTCTC 1 cut(s) 493
BsnI GGCC 2 cut(s) 215, 231
Bso31I GGTCTC 1 cut(s) 493
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 32, 420
Bsp143I GATC 4 cut(s) 249, 314, 323, 524
BspANI GGCC 2 cut(s) 215, 231
BspCNI CTCAG 3 cut(s) 175, 180, 279
BspLI GGNNCC 3 cut(s) 189, 270, 293
BspMAI CTGCAG 1 cut(s) 122
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 1 cut(s) 322
BspQI GCTCTTC 1 cut(s) 408
BspT104I TTCGAA 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 76
BssMI GATC 4 cut(s) 249, 314, 323, 524
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 1 cut(s) 456
Bst6I CTCTTC 2 cut(s) 387, 408
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 74
BstDEI CTNAG 3 cut(s) 167, 183, 287
BstENI CCTNNNNNAGG 1 cut(s) 351
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 4 cut(s) 252, 317, 326, 527
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 4 cut(s) 249, 314, 323, 524
BstMWI GCNNNNNNNGC 3 cut(s) 78, 228, 317
BstNI CCWGG 1 cut(s) 296
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 118
BstV1I GCAGC 2 cut(s) 132, 377
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
BsuRI GGCC 2 cut(s) 215, 231
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 1 cut(s) 74
CfoI GCGC 1 cut(s) 148
Csp6I GTAC 3 cut(s) 100, 164, 429
CviAII CATG 2 cut(s) 136, 362
CviJI RGCY 7 cut(s) 30, 215, 231, 286, 304, 418, 586
CviKI_1 RGCY 7 cut(s) 30, 215, 231, 286, 304, 418, 586
CviQI GTAC 3 cut(s) 100, 164, 429
DdeI CTNAG 3 cut(s) 167, 183, 287
DpnI GATC 4 cut(s) 251, 316, 325, 526
DpnII GATC 4 cut(s) 249, 314, 323, 524
DriI GACNNNNNGTC 1 cut(s) 136
Eam1104I CTCTTC 2 cut(s) 387, 408
Eam1105I GACNNNNNGTC 1 cut(s) 136
EarI CTCTTC 2 cut(s) 387, 408
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 2 cut(s) 32, 420
Eco31I GGTCTC 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 30
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoRII CCWGG 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 32, 420
FaeI CATG 2 cut(s) 139, 365
FaiI YATR 2 cut(s) 137, 363
FatI CATG 2 cut(s) 135, 361
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 2 cut(s) 121, 366
FriOI GRGCYC 2 cut(s) 32, 420
Fsp4HI GCNGC 2 cut(s) 121, 366
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 147
GluI GCNGC 2 cut(s) 121, 366
GsaI CCCAGC 3 cut(s) 26, 85, 152
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 2 cut(s) 215, 231
HapII CCGG 1 cut(s) 283
HhaI GCGC 1 cut(s) 148
Hin1II CATG 2 cut(s) 139, 365
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 2 cut(s) 177, 337
HpaII CCGG 1 cut(s) 283
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 2 cut(s) 431, 601
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 2 cut(s) 247, 469
Hpy8I GTNNAC 2 cut(s) 431, 601
HpyAV CCTTC 4 cut(s) 121, 347, 357, 576
HpyCH4III ACNGT 1 cut(s) 456
HpyCH4V TGCA 8 cut(s) 120, 222, 365, 437, 452, 510, 571, 592
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 228, 317
HpyF3I CTNAG 3 cut(s) 167, 183, 287
Hsp92II CATG 2 cut(s) 139, 365
HspAI GCGC 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 4 cut(s) 249, 314, 323, 524
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 1 cut(s) 27
LpnPI CCDG 8 cut(s) 8, 67, 134, 281, 296, 297, 308, 578
Lsp1109I GCAGC 2 cut(s) 132, 377
LweI GCATC 2 cut(s) 446, 558
MaeI CTAG 1 cut(s) 305
MaeIII GTNAC 2 cut(s) 224, 338
MalI GATC 4 cut(s) 251, 316, 325, 526
MboI GATC 4 cut(s) 249, 314, 323, 524
MboII GAAGA 3 cut(s) 374, 425, 591
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 2 cut(s) 32, 420
MlyI GAGTC 2 cut(s) 186, 346
MnlI CCTC 7 cut(s) 53, 87, 178, 242, 282, 512, 551
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 3 cut(s) 390, 516, 536
MspI CCGG 1 cut(s) 283
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 3 cut(s) 78, 228, 317
NdeII GATC 4 cut(s) 249, 314, 323, 524
NlaIII CATG 2 cut(s) 139, 365
NlaIV GGNNCC 3 cut(s) 189, 270, 293
NmuCI GTSAC 2 cut(s) 224, 338
NspI RCATGY 1 cut(s) 139
NspV TTCGAA 1 cut(s) 111
PciSI GCTCTTC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 357
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 2 cut(s) 122, 367
PleI GAGTC 2 cut(s) 185, 345
PpsI GAGTC 2 cut(s) 185, 345
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 1 cut(s) 294
PspFI CCCAGC 3 cut(s) 22, 81, 148
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 189, 270, 293
PsrI GAACNNNNNNTAC 2 cut(s) 92, 124
PstI CTGCAG 1 cut(s) 122
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 3 cut(s) 101, 165, 430
RsaNI GTAC 3 cut(s) 100, 164, 429
SacI GAGCTC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 3 cut(s) 390, 516, 536
SatI GCNGC 2 cut(s) 121, 366
Sau3AI GATC 4 cut(s) 249, 314, 323, 524
ScaI AGTACT 1 cut(s) 101
SchI GAGTC 2 cut(s) 186, 346
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 2 cut(s) 32, 420
SetI ASST 5 cut(s) 32, 189, 293, 306, 597
SfaNI GCATC 2 cut(s) 446, 558
SfcI CTRYAG 1 cut(s) 118
SfuI TTCGAA 1 cut(s) 111
SspMI CTAG 1 cut(s) 305
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 294
TaaI ACNGT 1 cut(s) 456
TaqI TCGA 3 cut(s) 111, 400, 556
TatI WGTACW 1 cut(s) 99
Tru1I TTAA 3 cut(s) 390, 516, 536
Tru9I TTAA 3 cut(s) 390, 516, 536
TseFI GTSAC 2 cut(s) 224, 338
TseI GCWGC 2 cut(s) 120, 365
Tsp45I GTSAC 2 cut(s) 224, 338
TspDTI ATGAA 2 cut(s) 350, 426
XagI CCTNNNNNAGG 1 cut(s) 351
XceI RCATGY 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 1 cut(s) 305
ZrmI AGTACT 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.