pycom12g03590

Protein virilizer homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010141
Physical Location & Seq
Reverse (-)
3487221 .. 3502116
14896 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5001 bp
ATGGGTCGCCCCGAGCCTTGTGTGTTGTTCACCCAGACCTTCGTCCATCCCCATCTCGATGAATACGTCGACGAGGTTTTGTTTGCTGAGCCCATTGTCATCACTGCTTGCGAGTTCCTCGAACAGAACGTGTCGTTTGCTTCTCAGGCAGTATCACTTCTCGGGGCTACTTCACCTCCTTCATTTGCTTTGGAGGTATTTGTTCAATGTGAGGGGGAGACAAGGTTCAGGCGCCTGTGTCAGCCTTTCCTATATTCTCCTTCTTCTTCAAATGTGCTAGAAGTAGAGGCTGTTGTAACTAATCATCTCGTTGTAAGGGGCAGCTACCGCAGTCTGAGCTTAGTCATATATGGAAACACGGCAGAAGATCTAGGGCAATTTAACATTGAAATTGATGATAGCTCCATAACTAATCTTGTTAGCTCTACTGAGGGAAAGCTTGAAGACCTCCCTCTTGCTTTGCATTCAACTAATCTGACAACTGGGGAATCCATATCAGCTCTTAATACTCTATCTCTACCTGTTGCGGCATCAGATATATCTGTTGAAGCTAAGCATTTATTACAACTGATGTTGAAGGTTTTTGAGTTGCCCAATCTAGGTGATGCATTGCATAAAGTTGTCGCTGCTGTAGTAACAGCTGCCACTTCTTATGTCACCTCCTCTTGGGGAAGATCCAACGAGTGTGAAGAGTTGCACAATGTTCTTAGTGAGGCCAGAACAGAGGTTATGGAGCTCTATAAAGTCTTTAAACACAAATCAGGGAATGGCTTAGCTGAACCACATGAAGACAACGGGCATTTTGAGTTTGAGGCTGAATTAGCAAATTCTAAACAGTTGGTGGATGTGTTAAGTCAGTACTTTCAATTTAGTAGGGACTTCTTGAGTGTTGTGCATCACCAACTTCCGCAGAATACAAATGTCATGTTGGGGTTGAGTGTGGCTTTTCTTTTGTGCTCTGGAAGAGAGAGTTGCTTTCATTTTGTTAGTGGTGGGGGAATGGAGCAGCTTGTACATGCTTTTTGTTGTGACAAGCAGAATTCTACTGCTAGTACATTACTACTACTTGGAGTTGTTGAGAAGGCTACTCAGCATTCTTTTGGGTGTGAAGGATTTTTAGGTTGGTGGCCACGTGAAGACGAAAACATCCCATCTGGTGTCAGTGATGGTTATAGTAGATTGTTAAATCTGTTATTGCAAAAGCAGCGTCATGATCTTGCTTCTTGTGCAACTTACGTCCTTCATCGGTTAAGATTTTATGAGGTTGCTTCAAGATTCGAGTGGGCAGCTTTATCGGTATTGGGTGGTCTATCTACTGTTGGTAGAGTCACAAGTGGAACTCTGGACATGCTTATCTGTGCAAAGTCACAGCTTAAAAAGCTGTTGAAATTGATAAATTCACGTGGTCCAATTGAAGATCCTTCTCCAGTTGCCCAAGCAACCAAATCCTTGATTCTTGGTCAGACTGAAGGTTTGTTGTCATTCAAAGCATCCAATAACTTGATTGCTTCTTCAAATTGTTGTTTCTCAAATTCAGATATCGACATGCATTTGCTGGCTCTTCTCAAGGAGAGGGGATTTCTACCTCTATCAGTTGCAATATTGTCGTCATCCATATTGCGTTCAGAAGTCGGATGTGTGAGGGACATATTTGTGGATGTTGTGTCATCAATTGAGGCCATAATTCTCTCCCTTCTTTTTTGTCGCTCAGGTGCACTAAGGGGTGCTAATGATGTGAATAAGGATGCGTGTCTTCCTCTTCGATATGCATTTATTTCATTATCCAAAGGATTCTTTTGTGCTCCACAGGATGTTGGAATGATTGTTGGGGTCCATTTGAGGGTGGTTAATGCTGTAGACCGTTTGCTTACTGCAGCACCTAACTCTGAAGAATTTTTATGGGTGTTGTGGGAACTGTGTGGTCTTGCAAGGTCTGACTGTGGACGTCAAGCATTATTGGCTCTGGGGTATTTTCCGGAGGCTGTTAAAATTTTGATTGAAGCTTTACATTATGCCAAAGAAACAGAGCCGCTTGCTAAAAATAATGGAGCCTCGCCGTTAAATATTGCCATATTTCACTCAGCTGCTGAGATTTTTGAAGCGATTGTTTCTGATTCCACTGCATCTTCTCTTGGATCTTGGATTGGACATGTTATGGAGCTCCATAGGGCTTTACATTCCTCTTCTCCTGGGTCAAATAGGAAAGATGCCCCCACACGGCTGTTGGAATGGATAGATGCTGGTGTAGTATATCATAAAAATGGGGCAACAAGTCTTATACGGTATGCTGCTGTGTTGGCTTCTGGAGGAGATGCCCACTTGACTTCAAACATACCGCTGGTGGCAGATTTGGCAGATGTAGAGAATGCAGTTGGAGATACATCTTGTGGTTCTGATGTGAATGTTATGGAAAATCTTGGAAAGTTTATATCTGACAAGACTTTTGATGGTGTTAATCTTCGTGACTCTTCTGTGGTACAGTTGACAACAGCATTTCGCATTTTAGCGTTCATTTCAGAGAACTCAACTGTTGCTGCTACTTTGTATGATGAAGGTGTTATACCAATAATCTATGCAGTTCTGGTCAATTGTAGGTCTATGCTTGAGAGGTCCTCAAACAGTTATGACTACCTTGTTGATGAGGAGTGCAATACTACATCAGATTTATTGTCGGAACGTAATCATGAGCAGAGTTTAGTTGATCGTCTTATTCCTACAATTGTGTTATCGATCAATTTGTTGCAGAAATTGCAGGAAGTACAAGAGCAGCACAGAAATACCAAGTTATTGAATGCCCTTCTACGATTGCATAGAGAAGTAAGTCCAAAATTGGCTGCATGTGCAGCGGACTTATCCTCTCCATATCCTGTTTCTGCACTTGGTTTTGGAGCTATCTGTCATCTTCTGGTGTCGGCACTTGCTTGTTGGCCGGTCTATGGTTGGACTCCTGGCCTTTTCCACTCCCTCCTTGCTAATGTTCAAGTTCCTTCTTTGCTGGCCTTGGGTCCAAAGGAAACATGTAGTTTGCTTTGTCTCCTGAATGATTTATTTCCTGATGAAGGGGTCTGGCTCTGGAAAAATGGGATGCCCTTGTTGAGTGCCCTTAGAAAGTTGTCTGTTGGGACTGTTTTGGGTCCTCAGAAGGAGAGACAGGTCAATTGGTATTTGCATCATATGCACCTGGAGAAGATGCTCAGCCAATTGACGCCACATCTTGACAAAGTTGCCCAGATTATTCAACATTATGCTATCTCTGCACTGGTGGTCATTCAAGACATGCTTCGAGTCTTTATTATTCATATTGCTTGCCTAAGAGCTCAAAGTTTTCCCGTACTTTTACGACCCATATTCTCATGGATTCGTGATCATACGTATGATACATCTTCGCCATCAGATACGGATGCTTATAAGGTTTATAGATATATTGATTTCATTGCTAGCTTATTGGAGCATCCACGTGCCAAGTCACTATTATTGAAGGAGGGTGCATTTAAGTTGCTAATCAGAGTGTTGGATAGTTGTTTAGTTGCCACTGAGACAGAAGGAATAGAGAATCTTGATGGTAGAAGTTCAGCTACATTTGGATTCGGTTTGCTCAACTGGTGCCTCCCTGTATTCAAGTCCTTCTCATTGATTTTCATTCCTCAAGCATCTCCACACCATGCTGGGGGAAATGATTTGCACGAATTTGAAAAGTTGAGTGCTGAAGATTGCAAAATCTTTTTAAAGTATTTCCTCAGGTTTTGTCAGGTTCTACCAGTTGGAAAAGAATTACTTGCCTGTCTTACTGCTTTTAAAGAACTGGGTGCTTGCAGTGAAGGTCAAAAAGCTTTAGCAGCCACTTTATATCCTGCCCTCTCTGTTGAGGACCATGAATGGAGAAAGAGTCCCCCATTGTTAAGTTGCTGTAAAAACTTGTTAAGATCAGTTTACTCTAAAGATGGGTTGTCAAGTTATGGAATTGAGGCCGTCAATGCATTGTCTGTGGGATCTTTCCGCTTCTGCCTTGATGGGGAAAGATTGAATCCAGACATGGTTGTTGCGGTGAGACTCCTGTTCGGAGTGTCTGATGATGTTATAGCTGAAGCAGATGGTGTGCCTGATGAAAACTTGAGCTATATTCATGAATTGACCTCTCAATTGAAGACCATGGAGGCTGCCCATATTGCCGGCTCTGATACTCAAACCCTTCTGTATCAGGTTTTAGAATCTGCGAAATCATTATCATTGCTATTGCAAAAACCTAGTTCGTCGTTAAAGGTGGATGATGTCTTTTCTGTTGATTCCATACCTCTGCCACCAAATATTCTAGTTTCTTTAAATACCCATATAATGCCAGATGGTGGTGCTGAAATGGATTGTGATTGTCTTTACCAAGGATCACTTGGAGACAAGTTTCACTGGGAGTGTCCTGATAAATCATCAGAATCAAATCTTAAAAGGAAACAGCCGCCATCACTTGATGGCCCAAATAGGCGTGCAAGAGGAGAAAACTCCCTGGCTGAAACTGCAAATCAAAATGTGTTTCCACGAGGACTGGGTTCAACTACTGCGTCATTGGGTCCTACTCGTAGGGATACCTTTCGGCAGCGCAAACCAAATACCAGCAGGCCTCCTTCCATGCATGTTGATGACTATGTTGCAAGAGAAAGAAATGATGGCGTTTCTACTTCTAATGTAATAGCAGTCCAGCGGGTGGGATCTACTGGTGGGCGACCTCCGTCAATTCATGTAGATGAATTTATGGCCAGGCAAAGGGAACGCCAGAATCTAGTGTCGCCGGTTGTTGTGGATGCGGCTGTACCAGTGAAGAGTTCAACTCCTGTGAATGACACAGCTACAGAGAAGTTCAACAAGCCTAAACAATTGAAAGCAGATCTTGATGATGATCTTCAGGGCATTGACATAGTATTTGACGGTGAGGAGTCTGAACCTGATGACAAACTGCCCTTTCCTCAGCCGGATCGATCACCTGCAACAGCCTGCTCCTGTCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIR_N

Protein Analysis

1667

Amino Acids

182.63

Weight (kDa)

5.45

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIR_N PF15912 8 - 121 4.5e-07 Virilizer, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05680 AT3G05680
fragaria_vesca FvH4_6g20410 FvH4_6g20410 FvH4_6g20410
malus_domestica MD12G1041200.v1.1 MD12G1041400.v1.1 MD14G1040500.v1.1
prunus_persica Prupe.7G093800_v2.0.a1
pyrus_communis pycom12g03590 pycom14g03440
rosa_chinensis RchiOBHm_Chr3g0476141
rosa_laevigata RLG00000023788
rosa_multiflora Rmu_sc0000114.1_g000025
rosa_roxburghii Rroxscaffold_6G00405450
rosa_rugosa Rorug03G0153000
rosa_samantha Rh3AG204100 Rh3BG234300 Rh3CG229700 Rh3DG229700
rosa_wichuraiana Rw3G018550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3408
AarI CACCTGC 1 cut(s) 4981
AatII GACGTC 1 cut(s) 1948
Acc36I ACCTGC 1 cut(s) 4981
AccB1I GGYRCC 2 cut(s) 231, 3603
AccB7I CCANNNNNTGG 2 cut(s) 4343, 4696
AccI GTMKAC 2 cut(s) 69, 1857
AccIII TCCGGA 1 cut(s) 1975
AclWI GGATC 7 cut(s) 669, 1412, 2143, 3997, 4387, 4708, 4971
AcoI YGGCCR 3 cut(s) 1127, 2927, 4746
AcsI RAATTY 8 cut(s) 826, 1039, 1396, 1531, 1892, 1989, 3686, 4739
AcuI CTGAAG 5 cut(s) 1488, 1908, 3726, 4104, 4877
AcvI CACGTG 3 cut(s) 1133, 1403, 3458
AcyI GRCGYC 3 cut(s) 232, 1945, 3206
AfaI GTAC 7 cut(s) 860, 1014, 1054, 2478, 2760, 3333, 4803
AflIII ACRYGT 3 cut(s) 129, 2149, 3017
AjnI CCWGG 5 cut(s) 2188, 2947, 3180, 4497, 4748
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
Alw21I GWGCWC 6 cut(s) 738, 959, 1717, 1804, 2163, 3319
Alw26I GTCTC 6 cut(s) 212, 3038, 3142, 3530, 4042, 4383
Alw44I GTGCAC 1 cut(s) 1713
AlwI GGATC 7 cut(s) 669, 1412, 2143, 3997, 4387, 4708, 4971
AlwNI CAGNNNCTG 1 cut(s) 2087
Ama87I CYCGRG 2 cut(s) 11, 161
Aor13HI TCCGGA 1 cut(s) 1975
ApaLI GTGCAC 1 cut(s) 1713
ApoI RAATTY 8 cut(s) 826, 1039, 1396, 1531, 1892, 1989, 3686, 4739
ArsI GACNNNNNNTTYG 8 cut(s) 1192, 1224, 1456, 1488, 2426, 2458, 4895, 4927
AspLEI GCGC 2 cut(s) 234, 4593
AspS9I GGNCC 8 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4466, 4562
AsuHPI GGTGA 8 cut(s) 22, 165, 614, 649, 890, 4057, 4931, 4962
AsuNHI GCTAGC 1 cut(s) 3437
AvaI CYCGRG 2 cut(s) 11, 161
AvaII GGWCC 7 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4562
AxyI CCTNAGG 1 cut(s) 3737
BaeGI GKGCMC 2 cut(s) 1717, 3103
BalI TGGCCA 2 cut(s) 1129, 4748
BanI GGYRCC 2 cut(s) 231, 3603
BanII GRGCYC 4 cut(s) 93, 738, 2163, 3319
BarI GAAGNNNNNNTAC 4 cut(s) 2544, 2576, 3559, 3591
BauI CACGAG 1 cut(s) 4530
BbrPI CACGTG 3 cut(s) 1133, 1403, 3458
BbsI GAAGAC 5 cut(s) 450, 795, 1143, 1745, 4151
Bbv12I GWGCWC 6 cut(s) 738, 959, 1717, 1804, 2163, 3319
BbvCI CCTCAGC 1 cut(s) 4956
BceAI ACGGC 4 cut(s) 375, 2041, 2234, 3953
BcgI CGANNNNNNTGC 4 cut(s) 1275, 1309, 1587, 1621
BciT130I CCWGG 5 cut(s) 2190, 2949, 3182, 4499, 4750
BciVI GTATCC 1 cut(s) 4570
BclI TGATCA 1 cut(s) 3364
BcoDI GTCTC 6 cut(s) 212, 3038, 3142, 3530, 4042, 4383
BfaI CTAG 8 cut(s) 278, 371, 599, 1050, 3438, 4245, 4310, 4772
BfmI CTRYAG 4 cut(s) 630, 1854, 1872, 4839
BfoI RGCGCY 1 cut(s) 235
BfuAI ACCTGC 1 cut(s) 4981
BfuI GTATCC 1 cut(s) 4570
BglII AGATCT 2 cut(s) 367, 4876
BlpI GCTNAGC 4 cut(s) 87, 552, 772, 3194
BmcAI AGTACT 1 cut(s) 860
Bme1390I CCNGG 5 cut(s) 2190, 2949, 3182, 4499, 4750
Bme18I GGWCC 7 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4562
BmeT110I CYCGRG 2 cut(s) 11, 161
BmgT120I GGNCC 8 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4466, 4562
BmiI GGNNCC 7 cut(s) 233, 1832, 2050, 3006, 3135, 3605, 4563
BmrFI CCNGG 5 cut(s) 2190, 2949, 3182, 4499, 4750
BmrI ACTGGG 4 cut(s) 492, 3812, 4411, 4547
BmtI GCTAGC 1 cut(s) 3441
BmuI ACTGGG 4 cut(s) 492, 3812, 4411, 4547
BoxI GACNNNNGTC 2 cut(s) 41, 4720
BpiI GAAGAC 5 cut(s) 450, 795, 1143, 1745, 4151
BplI GAGNNNNNCTC 4 cut(s) 2597, 2629, 4804, 4836
BpmI CTGGAG 3 cut(s) 1410, 2325, 3203
Bpu10I CCTNAGC 2 cut(s) 1708, 4956
Bpu1102I GCTNAGC 4 cut(s) 87, 552, 772, 3194
BpuEI CTTGAG 5 cut(s) 904, 1550, 2624, 3630, 4132
Bsa29I ATCGAT 2 cut(s) 2729, 4966
BsaAI YACGTR 4 cut(s) 1133, 1403, 3372, 3458
BsaBI GATNNNNATC 1 cut(s) 4887
BsaHI GRCGYC 3 cut(s) 232, 1945, 3206
BsaJI CCNNGG 5 cut(s) 2189, 3000, 4149, 4375, 4497
BsaWI WCCGGW 1 cut(s) 1975
BsaXI ACNNNNNCTCC 6 cut(s) 160, 190, 209, 239, 3871, 3901
Bse118I RCCGGY 3 cut(s) 2929, 4169, 4780
Bse21I CCTNAGG 1 cut(s) 3737
Bse3DI GCAATG 3 cut(s) 608, 3432, 4226
Bse8I GATNNNNATC 1 cut(s) 4887
BseAI TCCGGA 1 cut(s) 1975
BseBI CCWGG 5 cut(s) 2190, 2949, 3182, 4499, 4750
BseCI ATCGAT 2 cut(s) 2729, 4966
BseDI CCNNGG 5 cut(s) 2189, 3000, 4149, 4375, 4497
BseJI GATNNNNATC 1 cut(s) 4887
BseMI GCAATG 3 cut(s) 608, 3432, 4226
BseRI GAGGAG 5 cut(s) 652, 2322, 2657, 4500, 4937
BseSI GKGCMC 2 cut(s) 1717, 3103
BseYI CCCAGC 1 cut(s) 3665
BsgI GTGCAG 3 cut(s) 2859, 2862, 3240
BshNI GGYRCC 2 cut(s) 231, 3603
BshVI ATCGAT 2 cut(s) 2729, 4966
BsiHKAI GWGCWC 6 cut(s) 738, 959, 1717, 1804, 2163, 3319
BsiHKCI CYCGRG 2 cut(s) 11, 161
BsiSI CCGG 5 cut(s) 1976, 2930, 4170, 4781, 4961
BslFI GGGAC 4 cut(s) 890, 1658, 3136, 3873
BsmAI GTCTC 6 cut(s) 212, 3038, 3142, 3530, 4042, 4383
BsmFI GGGAC 4 cut(s) 890, 1658, 3136, 3873
BsmI GAATGC 4 cut(s) 463, 1093, 2371, 2797
BsoBI CYCGRG 2 cut(s) 11, 161
Bsp1286I GDGCHC 8 cut(s) 93, 738, 959, 1717, 1804, 2163, 3103, 3319
Bsp13I TCCGGA 1 cut(s) 1975
Bsp1407I TGTACA 1 cut(s) 1012
Bsp1720I GCTNAGC 4 cut(s) 87, 552, 772, 3194
Bsp19I CCATGG 1 cut(s) 4149
BspDI ATCGAT 2 cut(s) 2729, 4966
BspEI TCCGGA 1 cut(s) 1975
BspHI TCATGA 3 cut(s) 1210, 2683, 4123
BspLI GGNNCC 7 cut(s) 233, 1832, 2050, 3006, 3135, 3605, 4563
BspMAI CTGCAG 1 cut(s) 1876
BspMI ACCTGC 1 cut(s) 4981
BspOI GCTAGC 1 cut(s) 3441
BspPI GGATC 7 cut(s) 669, 1412, 2143, 3997, 4387, 4708, 4971
BspQI GCTCTTC 1 cut(s) 1566
BspT107I GGYRCC 2 cut(s) 231, 3603
BsrDI GCAATG 3 cut(s) 608, 3432, 4226
BsrFI RCCGGY 3 cut(s) 2929, 4169, 4780
BsrGI TGTACA 1 cut(s) 1012
BssAI RCCGGY 3 cut(s) 2929, 4169, 4780
BssECI CCNNGG 5 cut(s) 2189, 3000, 4149, 4375, 4497
BssNI GRCGYC 3 cut(s) 232, 1945, 3206
BssSI CACGAG 1 cut(s) 4530
BssT1I CCWWGG 3 cut(s) 3000, 4149, 4375
Bst2BI CACGAG 1 cut(s) 4530
Bst2UI CCWGG 5 cut(s) 2190, 2949, 3182, 4499, 4750
Bst6I CTCTTC 7 cut(s) 684, 958, 1566, 1764, 2188, 2473, 4805
BstACI GRCGYC 3 cut(s) 232, 1945, 3206
BstAPI GCANNNNNTGC 2 cut(s) 2749, 3175
BstAUI TGTACA 1 cut(s) 1012
BstBAI YACGTR 4 cut(s) 1133, 1403, 3372, 3458
BstDSI CCRYGG 1 cut(s) 4149
BstENI CCTNNNNNAGG 1 cut(s) 3057
BstH2I RGCGCY 1 cut(s) 235
BstHHI GCGC 2 cut(s) 234, 4593
BstMAI GTCTC 6 cut(s) 212, 3038, 3142, 3530, 4042, 4383
BstNI CCWGG 5 cut(s) 2190, 2949, 3182, 4499, 4750
BstNSI RCATGY 8 cut(s) 1019, 1351, 1549, 2153, 2841, 3021, 3280, 4628
BstPAI GACNNNNGTC 2 cut(s) 41, 4720
BstSCI CCNGG 5 cut(s) 2188, 2947, 3180, 4497, 4748
BstSFI CTRYAG 4 cut(s) 630, 1854, 1872, 4839
BstSLI GKGCMC 2 cut(s) 1717, 3103
BstSNI TACGTA 1 cut(s) 3372
BstV2I GAAGAC 5 cut(s) 450, 795, 1143, 1745, 4151
BstX2I RGATCY 7 cut(s) 367, 674, 1417, 2135, 3989, 4700, 4876
BstYI RGATCY 7 cut(s) 367, 674, 1417, 2135, 3989, 4700, 4876
Bsu15I ATCGAT 2 cut(s) 2729, 4966
Bsu36I CCTNAGG 1 cut(s) 3737
BsuI GTATCC 1 cut(s) 4570
BsuTUI ATCGAT 2 cut(s) 2729, 4966
BtgI CCRYGG 1 cut(s) 4149
BtsI GCAGTG 3 cut(s) 102, 2118, 3820
BtsIMutI CAGTG 8 cut(s) 102, 1168, 2118, 3257, 3531, 3820, 4399, 4812
BveI ACCTGC 1 cut(s) 4981
CaiI CAGNNNCTG 1 cut(s) 2087
CciI TCATGA 3 cut(s) 1210, 2683, 4123
CfoI GCGC 2 cut(s) 234, 4593
Cfr10I RCCGGY 3 cut(s) 2929, 4169, 4780
Cfr13I GGNCC 8 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4466, 4562
ClaI ATCGAT 2 cut(s) 2729, 4966
CseI GACGC 3 cut(s) 1196, 3214, 4542
Csp6I GTAC 7 cut(s) 859, 1013, 1053, 2477, 2759, 3332, 4802
CviQI GTAC 7 cut(s) 859, 1013, 1053, 2477, 2759, 3332, 4802
DinI GGCGCC 1 cut(s) 233
DraI TTTAAA 4 cut(s) 751, 3726, 3796, 4320
EaeI YGGCCR 3 cut(s) 1127, 2927, 4746
Eam1104I CTCTTC 7 cut(s) 684, 958, 1566, 1764, 2188, 2473, 4805
EarI CTCTTC 7 cut(s) 684, 958, 1566, 1764, 2188, 2473, 4805
Ecl136II GAGCTC 3 cut(s) 736, 2161, 3317
Eco105I TACGTA 1 cut(s) 3372
Eco130I CCWWGG 3 cut(s) 3000, 4149, 4375
Eco147I AGGCCT 1 cut(s) 4612
Eco24I GRGCYC 4 cut(s) 93, 738, 2163, 3319
Eco32I GATATC 1 cut(s) 1540
Eco47I GGWCC 7 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4562
Eco53kI GAGCTC 3 cut(s) 736, 2161, 3317
Eco57I CTGAAG 5 cut(s) 1488, 1908, 3726, 4104, 4877
Eco72I CACGTG 3 cut(s) 1133, 1403, 3458
Eco81I CCTNAGG 1 cut(s) 3737
Eco88I CYCGRG 2 cut(s) 11, 161
EcoICRI GAGCTC 3 cut(s) 736, 2161, 3317
EcoNI CCTNNNNNAGG 1 cut(s) 3057
EcoO109I RGGNCCY 3 cut(s) 2610, 3134, 4562
EcoRI GAATTC 1 cut(s) 1039
EcoRII CCWGG 5 cut(s) 2188, 2947, 3180, 4497, 4748
EcoRV GATATC 1 cut(s) 1540
EcoT14I CCWWGG 3 cut(s) 3000, 4149, 4375
EcoT22I ATGCAT 5 cut(s) 610, 1551, 1771, 3979, 4626
EcoT38I GRGCYC 4 cut(s) 93, 738, 2163, 3319
EgeI GGCGCC 1 cut(s) 233
EheI GGCGCC 1 cut(s) 233
ErhI CCWWGG 3 cut(s) 3000, 4149, 4375
FalI AAGNNNNNCTT 8 cut(s) 3264, 3296, 3759, 3791, 4368, 4400, 4863, 4895
FaqI GGGAC 4 cut(s) 890, 1658, 3136, 3873
FauI CCCGC 1 cut(s) 4686
FauNDI CATATG 1 cut(s) 3174
FbaI TGATCA 1 cut(s) 3364
FblI GTMKAC 2 cut(s) 69, 1857
FriOI GRGCYC 4 cut(s) 93, 738, 2163, 3319
FspBI CTAG 8 cut(s) 278, 371, 599, 1050, 3438, 4245, 4310, 4772
GlaI GCGC 2 cut(s) 233, 4592
GsaI CCCAGC 1 cut(s) 3669
GsuI CTGGAG 3 cut(s) 1410, 2325, 3203
HaeII RGCGCY 1 cut(s) 235
HapII CCGG 5 cut(s) 1976, 2930, 4170, 4781, 4961
HgaI GACGC 3 cut(s) 1196, 3214, 4542
HhaI GCGC 2 cut(s) 234, 4593
Hin1I GRCGYC 3 cut(s) 232, 1945, 3206
Hin6I GCGC 2 cut(s) 232, 4591
HinP1I GCGC 2 cut(s) 232, 4591
HincII GTYRAC 2 cut(s) 70, 2484
HindII GTYRAC 2 cut(s) 70, 2484
HindIII AAGCTT 3 cut(s) 437, 2001, 3828
HpaII CCGG 5 cut(s) 1976, 2930, 4170, 4781, 4961
HphI GGTGA 8 cut(s) 22, 165, 614, 649, 890, 4057, 4931, 4962
Hpy166II GTNNAC 7 cut(s) 30, 70, 1715, 1858, 1943, 2484, 3931
Hpy8I GTNNAC 7 cut(s) 30, 70, 1715, 1858, 1943, 2484, 3931
Hpy99I CGWCG 3 cut(s) 71, 74, 4255
HpyCH4IV ACGT 9 cut(s) 66, 129, 1132, 1236, 1402, 1945, 2677, 3371, 3457
HpySE526I ACGT 9 cut(s) 66, 129, 1132, 1236, 1402, 1945, 2677, 3371, 3457
Hsp92I GRCGYC 3 cut(s) 232, 1945, 3206
HspAI GCGC 2 cut(s) 232, 4591
KasI GGCGCC 1 cut(s) 231
Kpn2I TCCGGA 1 cut(s) 1975
KroI GCCGGC 1 cut(s) 4169
KroNI GCCGGC 1 cut(s) 4171
Ksp22I TGATCA 1 cut(s) 3364
LguI GCTCTTC 1 cut(s) 1566
MaeI CTAG 8 cut(s) 278, 371, 599, 1050, 3438, 4245, 4310, 4772
MaeII ACGT 9 cut(s) 66, 129, 1132, 1236, 1402, 1945, 2677, 3371, 3457
MaeIII GTNAC 8 cut(s) 295, 634, 655, 1028, 1327, 1365, 2462, 3465
MfeI CAATTG 8 cut(s) 1410, 1671, 2587, 2718, 3157, 3200, 4139, 4865
MflI RGATCY 7 cut(s) 367, 674, 1417, 2135, 3989, 4700, 4876
MhlI GDGCHC 8 cut(s) 93, 738, 959, 1717, 1804, 2163, 3103, 3319
MlsI TGGCCA 2 cut(s) 1129, 4748
MluNI TGGCCA 2 cut(s) 1129, 4748
Mly113I GGCGCC 1 cut(s) 232
MlyI GAGTC 7 cut(s) 1335, 2459, 2938, 3294, 3895, 4044, 4934
MmeI TCCRAC 9 cut(s) 702, 1612, 1795, 2205, 2353, 2652, 2921, 3492, 3742
Mox20I TGGCCA 2 cut(s) 1129, 4748
Mph1103I ATGCAT 5 cut(s) 610, 1551, 1771, 3979, 4626
MroI TCCGGA 1 cut(s) 1975
MroNI GCCGGC 1 cut(s) 4169
MscI TGGCCA 2 cut(s) 1129, 4748
MslI CAYNNNNRTG 7 cut(s) 57, 1652, 2259, 3372, 3456, 4629, 4734
Msp20I TGGCCA 2 cut(s) 1129, 4748
MspA1I CMGCKG 5 cut(s) 641, 2084, 2338, 2846, 4693
MspI CCGG 5 cut(s) 1976, 2930, 4170, 4781, 4961
MspR9I CCNGG 5 cut(s) 2190, 2949, 3182, 4499, 4750
MunI CAATTG 8 cut(s) 1410, 1671, 2587, 2718, 3157, 3200, 4139, 4865
Mva1269I GAATGC 4 cut(s) 463, 1093, 2371, 2797
MvaI CCWGG 5 cut(s) 2190, 2949, 3182, 4499, 4750
NaeI GCCGGC 1 cut(s) 4171
NarI GGCGCC 1 cut(s) 232
NcoI CCATGG 1 cut(s) 4149
NdeI CATATG 1 cut(s) 3174
NgoMIV GCCGGC 1 cut(s) 4169
NheI GCTAGC 1 cut(s) 3437
NlaIV GGNNCC 7 cut(s) 233, 1832, 2050, 3006, 3135, 3605, 4563
NmuCI GTSAC 6 cut(s) 655, 1028, 1327, 1365, 2462, 3465
NsiI ATGCAT 5 cut(s) 610, 1551, 1771, 3979, 4626
NspI RCATGY 8 cut(s) 1019, 1351, 1549, 2153, 2841, 3021, 3280, 4628
PagI TCATGA 3 cut(s) 1210, 2683, 4123
PaqCI CACCTGC 1 cut(s) 4981
PceI AGGCCT 1 cut(s) 4612
PciI ACATGT 2 cut(s) 2149, 3017
PciSI GCTCTTC 1 cut(s) 1566
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 126
PctI GAATGC 4 cut(s) 463, 1093, 2371, 2797
PdiI GCCGGC 1 cut(s) 4171
PflMI CCANNNNNTGG 2 cut(s) 4343, 4696
PleI GAGTC 7 cut(s) 1334, 2459, 2938, 3293, 3894, 4044, 4933
PluTI GGCGCC 1 cut(s) 235
PmaCI CACGTG 3 cut(s) 1133, 1403, 3458
PmlI CACGTG 3 cut(s) 1133, 1403, 3458
PpsI GAGTC 7 cut(s) 1334, 2459, 2938, 3293, 3894, 4044, 4933
Ppu21I YACGTR 4 cut(s) 1133, 1403, 3372, 3458
PpuMI RGGWCCY 3 cut(s) 2610, 3134, 4562
PscI ACATGT 2 cut(s) 2149, 3017
PshAI GACNNNNGTC 2 cut(s) 41, 4720
PsiI TTATAA 1 cut(s) 3408
Psp124BI GAGCTC 3 cut(s) 738, 2163, 3319
Psp5II RGGWCCY 3 cut(s) 2610, 3134, 4562
Psp6I CCWGG 5 cut(s) 2188, 2947, 3180, 4497, 4748
PspCI CACGTG 3 cut(s) 1133, 1403, 3458
PspFI CCCAGC 1 cut(s) 3665
PspGI CCWGG 5 cut(s) 2188, 2947, 3180, 4497, 4748
PspN4I GGNNCC 7 cut(s) 233, 1832, 2050, 3006, 3135, 3605, 4563
PspPI GGNCC 8 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4466, 4562
PspPPI RGGWCCY 3 cut(s) 2610, 3134, 4562
PstI CTGCAG 1 cut(s) 1876
PstNI CAGNNNCTG 1 cut(s) 2087
PsuI RGATCY 7 cut(s) 367, 674, 1417, 2135, 3989, 4700, 4876
PvuII CAGCTG 2 cut(s) 641, 2084
RsaI GTAC 7 cut(s) 860, 1014, 1054, 2478, 2760, 3333, 4803
RsaNI GTAC 7 cut(s) 859, 1013, 1053, 2477, 2759, 3332, 4802
RseI CAYNNNNRTG 7 cut(s) 57, 1652, 2259, 3372, 3456, 4629, 4734
SacI GAGCTC 3 cut(s) 738, 2163, 3319
SalI GTCGAC 1 cut(s) 68
SapI GCTCTTC 1 cut(s) 1566
Sau96I GGNCC 8 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4466, 4562
ScaI AGTACT 1 cut(s) 860
SchI GAGTC 7 cut(s) 1335, 2459, 2938, 3294, 3895, 4044, 4934
ScrFI CCNGG 5 cut(s) 2190, 2949, 3182, 4499, 4750
SduI GDGCHC 8 cut(s) 93, 738, 959, 1717, 1804, 2163, 3103, 3319
SfcI CTRYAG 4 cut(s) 630, 1854, 1872, 4839
SfoI GGCGCC 1 cut(s) 233
SgrDI CGTCGACG 1 cut(s) 68
SinI GGWCC 7 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4562
SmiMI CAYNNNNRTG 7 cut(s) 57, 1652, 2259, 3372, 3456, 4629, 4734
SmlI CTYRAG 5 cut(s) 883, 1565, 2603, 3645, 4111
SmoI CTYRAG 5 cut(s) 883, 1565, 2603, 3645, 4111
SnaBI TACGTA 1 cut(s) 3372
SseBI AGGCCT 1 cut(s) 4612
SspDI GGCGCC 1 cut(s) 231
SspI AATATT 3 cut(s) 1602, 2065, 4306
SspMI CTAG 8 cut(s) 278, 371, 599, 1050, 3438, 4245, 4310, 4772
SstI GAGCTC 3 cut(s) 738, 2163, 3319
StuI AGGCCT 1 cut(s) 4612
StyD4I CCNGG 5 cut(s) 2188, 2947, 3180, 4497, 4748
StyI CCWWGG 3 cut(s) 3000, 4149, 4375
TaiI ACGT 9 cut(s) 69, 132, 1135, 1239, 1405, 1948, 2680, 3374, 3460
TaqI TCGA 9 cut(s) 57, 69, 120, 1278, 1542, 1762, 2729, 3283, 4966
TatI WGTACW 4 cut(s) 858, 1012, 1052, 2758
TauI GCSGC 4 cut(s) 530, 2032, 4453, 4799
TscAI CASTG 8 cut(s) 109, 1168, 2125, 3264, 3538, 3820, 4406, 4812
TseFI GTSAC 6 cut(s) 655, 1028, 1327, 1365, 2462, 3465
Tsp45I GTSAC 6 cut(s) 655, 1028, 1327, 1365, 2462, 3465
TspGWI ACGGA 2 cut(s) 3413, 4710
TspRI CASTG 8 cut(s) 109, 1168, 2125, 3264, 3538, 3820, 4406, 4812
Van91I CCANNNNNTGG 2 cut(s) 4343, 4696
VneI GTGCAC 1 cut(s) 1713
VpaK11BI GGWCC 7 cut(s) 1406, 1831, 2610, 3005, 3134, 3868, 4562
XagI CCTNNNNNAGG 1 cut(s) 3057
XapI RAATTY 8 cut(s) 826, 1039, 1396, 1531, 1892, 1989, 3686, 4739
XceI RCATGY 8 cut(s) 1019, 1351, 1549, 2153, 2841, 3021, 3280, 4628
XcmI CCANNNNNNNNNTGG 2 cut(s) 2221, 4382
XmiI GTMKAC 2 cut(s) 69, 1857
XspI CTAG 8 cut(s) 278, 371, 599, 1050, 3438, 4245, 4310, 4772
ZraI GACGTC 1 cut(s) 1946
ZrmI AGTACT 1 cut(s) 860
Zsp2I ATGCAT 5 cut(s) 610, 1551, 1771, 3979, 4626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.