pycom12g03710

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008328
Physical Location & Seq
Reverse (-)
3612286 .. 3614671
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1371 bp
ATGGTGGTGAAGATGATGAGGTGGCGACCGTGGCCGCCTCTGACGACGAAGAAGTACGAGGTGAGGCTGGTGGTGCGGCGGTTGGAGGGCTGGGATCTGGTGCGGGAGGGTGCAGGCGGGGCCGGTCCACAGGACCAAGAGAAAGAGGACAAGTGGACGGCGGAGATTAGGTGGAAGGGATCCAAGGTCAAGGTTGGGGCTTTGAGCTCTCTCCGTCGCGCCGTCGTCAAGAGGAACTTTACCAGAGAGGTCGAGGCTTCGTCCCAAGACGGCGTCGTTTCCTGGGACGAGGAGTTTCACAGCGTTTGCACTCTCTCGGCTTACAAGGACAATGTATTTCACCCTTGGGAGATCGTATTTACTGTGTTCAATGGTTTGAATCAAGGTGCCAAAAGTAAGGCTCCTGCTGTTGGAAAGGCATCAGTGAACCTTGCTGATTTTGTTTCGGAAGCTGAACAGAAGGAGCTTGAGTTACACATCCCCTTCACCTCATCTGGTCCTGCTGCTGAACTTTGTATATCACTCAGCTTATTGGATCTGGGAACTACTCAGGAAATTACAGAGCCAGTGGAGAGATCAATTTTGCCTGTTCAATCACCTCCTCAGTCAGCAGAAACTGTATCGCCAGAAAAAGACGAGCTTTCTGCTCTGAAAGCTGGTCTTAGAAAAGTAAAAATTTTCACAGAATATGTGTCTGCCAGAAAAGCAAAAAAGCCCTGCCGTGAGGAGGATGGCAGTGAGGGCAGGTGTTCTGCTAGGAGTGAGGATGGTGATTATAATTATCCTTTTGACTCTGAGTCACTTGATGAGTTTGAGGAAGGTGAATCAGAAGAGGTCAAAGAAGATTCCAGCGTTAGGAAGTCATTCAGTTATGGCACGCTGGCTCATGCAAATTATGCTGGAGGATCAATTTACTCCAATATGAGAATCAACGATGAAGGTGAGGACTGGGTTTACTACAGCAATCGCAAATCAGATGTGGGATGCTCACAAACTGAGGAGTCCACTGCACCAGTCTCTGACCCGCCTGCAAGTTCAAAACGAGGCTTACTATCTTGGAGGAAGAGAAAGCTGAGCTTCATCCGGTCTCCTAAAGCCAAAGGAGAACCTTTGTTAAAGAAGGCCTTTGGTGAAGAAGGTGGTGATGACATTGATTTTGATCGTCGGCAGCTTAGCTCTGATGAAATTCTATCTCTCGGGTGGAATAAGACAGAGGAGGATTCATCTGCTAATCGGTCGTCAGTGTCTGAATTTGGGGATGATAATTTTGCCATCGGCAGTTGGGAGCACAAGGAAGTGACAAACCGCGATGGACACATGAAGCTTCACACCCAAATCTTTTCTGCTTCTATTGATCGATCAACGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

457

Amino Acids

50.95

Weight (kDa)

5.23

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 85 - 180 2.4e-10 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 777
AarI CACCTGC 1 cut(s) 735
Acc36I ACCTGC 1 cut(s) 735
AccB1I GGYRCC 1 cut(s) 386
AccII CGCG 2 cut(s) 219, 1308
AciI CCGC 8 cut(s) 35, 76, 79, 103, 117, 161, 1025, 1306
AclWI GGATC 5 cut(s) 102, 174, 187, 543, 913
AcoI YGGCCR 1 cut(s) 32
AcsI RAATTY 3 cut(s) 675, 1185, 1250
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 3 cut(s) 410, 727, 855
AgsI TTSAA 4 cut(s) 370, 379, 593, 1038
AhdI GACNNNNNGTC 1 cut(s) 796
AjnI CCWGG 1 cut(s) 281
Alw21I GWGCWC 2 cut(s) 209, 1290
Alw26I GTCTC 2 cut(s) 1021, 1092
AlwI GGATC 5 cut(s) 102, 174, 187, 543, 913
AlwNI CAGNNNCTG 3 cut(s) 617, 1019, 1247
Ama87I CYCGRG 1 cut(s) 1196
AoxI GGCC 3 cut(s) 32, 120, 1122
ApeKI GCWGC 2 cut(s) 503, 1168
ApoI RAATTY 3 cut(s) 675, 1185, 1250
ArsI GACNNNNNNTTYG 2 cut(s) 1139, 1171
Asp700I GAANNNNTTC 1 cut(s) 863
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 4 cut(s) 120, 125, 133, 497
AvaI CYCGRG 1 cut(s) 1196
AvaII GGWCC 3 cut(s) 125, 133, 497
BamHI GGATCC 1 cut(s) 179
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 209
Bbv12I GWGCWC 2 cut(s) 209, 1290
BbvI GCAGC 2 cut(s) 490, 1180
BccI CCATC 4 cut(s) 725, 761, 1280, 1304
BceAI ACGGC 4 cut(s) 174, 206, 286, 705
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 1 cut(s) 283
BcoDI GTCTC 2 cut(s) 1021, 1092
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 1 cut(s) 958
BfuAI ACCTGC 1 cut(s) 735
BisI GCNGC 4 cut(s) 35, 77, 504, 1169
BlpI GCTNAGC 2 cut(s) 1073, 1172
BlsI GCNGC 4 cut(s) 36, 78, 505, 1170
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 3 cut(s) 125, 133, 497
BmeRI GACNNNNNGTC 1 cut(s) 796
BmeT110I CYCGRG 1 cut(s) 1196
BmgT120I GGNCC 4 cut(s) 120, 125, 133, 497
BmiI GGNNCC 4 cut(s) 121, 181, 388, 402
BmrFI CCNGG 1 cut(s) 283
BmrI ACTGGG 1 cut(s) 958
BmsI GCATC 2 cut(s) 428, 974
BmuI ACTGGG 1 cut(s) 958
BpmI CTGGAG 1 cut(s) 921
Bpu1102I GCTNAGC 2 cut(s) 1073, 1172
BpuEI CTTGAG 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 1357
BsaBI GATNNNNATC 1 cut(s) 1158
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 1092
BsaJI CCNNGG 4 cut(s) 29, 183, 282, 344
BsaWI WCCGGW 1 cut(s) 1083
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 455, 485
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 727, 855
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 3 cut(s) 566, 953, 1013
Bse8I GATNNNNATC 1 cut(s) 1158
BseBI CCWGG 1 cut(s) 283
BseCI ATCGAT 1 cut(s) 1357
BseDI CCNNGG 4 cut(s) 29, 183, 282, 344
BseGI GGATG 6 cut(s) 477, 736, 772, 989, 1080, 1264
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 3 cut(s) 410, 727, 855
BseMII CTCAG 6 cut(s) 538, 563, 617, 786, 987, 1064
BseNI ACTGG 3 cut(s) 566, 953, 1013
BseRI GAGGAG 5 cut(s) 305, 591, 740, 1013, 1229
BseXI GCAGC 2 cut(s) 490, 1180
BseYI CCCAGC 1 cut(s) 90
BsgI GTGCAG 2 cut(s) 132, 993
Bsh1236I CGCG 2 cut(s) 219, 1308
Bsh1285I CGRYCG 2 cut(s) 29, 1238
BshFI GGCC 3 cut(s) 34, 122, 1124
BshNI GGYRCC 1 cut(s) 386
BshVI ATCGAT 1 cut(s) 1357
BsiEI CGRYCG 2 cut(s) 29, 1238
BsiHKAI GWGCWC 2 cut(s) 209, 1290
BsiHKCI CYCGRG 1 cut(s) 1196
BsiSI CCGG 2 cut(s) 123, 1084
BslFI GGGAC 2 cut(s) 247, 299
BslI CCNNNNNNNGG 3 cut(s) 410, 727, 855
BsmAI GTCTC 2 cut(s) 1021, 1092
BsmFI GGGAC 2 cut(s) 247, 299
BsnI GGCC 3 cut(s) 34, 122, 1124
Bso31I GGTCTC 1 cut(s) 1092
BsoBI CYCGRG 1 cut(s) 1196
Bsp1286I GDGCHC 2 cut(s) 209, 1290
Bsp143I GATC 9 cut(s) 94, 179, 351, 535, 575, 905, 1159, 1354, 1358
Bsp1720I GCTNAGC 2 cut(s) 1073, 1172
BspACI CCGC 8 cut(s) 35, 76, 79, 103, 117, 161, 1025, 1306
BspANI GGCC 3 cut(s) 34, 122, 1124
BspCNI CTCAG 6 cut(s) 537, 562, 616, 787, 988, 1065
BspDI ATCGAT 1 cut(s) 1357
BspFNI CGCG 2 cut(s) 219, 1308
BspLI GGNNCC 4 cut(s) 121, 181, 388, 402
BspMI ACCTGC 1 cut(s) 735
BspPI GGATC 5 cut(s) 102, 174, 187, 543, 913
BspT107I GGYRCC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 1092
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 3 cut(s) 566, 953, 1013
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 29, 183, 282, 344
BssMI GATC 9 cut(s) 94, 179, 351, 535, 575, 905, 1159, 1354, 1358
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 2 cut(s) 183, 344
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 3 cut(s) 30, 364, 619
Bst6I CTCTTC 2 cut(s) 825, 1058
BstACI GRCGYC 1 cut(s) 273
BstAPI GCANNNNNTGC 1 cut(s) 896
BstC8I GCNNGC 4 cut(s) 115, 878, 882, 1029
BstDEI CTNAG 8 cut(s) 524, 549, 603, 662, 795, 996, 1073, 1172
BstDSI CCRYGG 1 cut(s) 29
BstF5I GGATG 6 cut(s) 477, 736, 772, 989, 1080, 1264
BstFNI CGCG 2 cut(s) 219, 1308
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 9 cut(s) 97, 182, 354, 538, 578, 908, 1162, 1357, 1361
BstMAI GTCTC 2 cut(s) 1021, 1092
BstMBI GATC 9 cut(s) 94, 179, 351, 535, 575, 905, 1159, 1354, 1358
BstMCI CGRYCG 2 cut(s) 29, 1238
BstMWI GCNNNNNNNGC 7 cut(s) 31, 73, 119, 653, 704, 741, 896
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstSFI CTRYAG 1 cut(s) 958
BstUI CGCG 2 cut(s) 219, 1308
BstV1I GCAGC 2 cut(s) 490, 1180
BstX2I RGATCY 3 cut(s) 94, 179, 535
BstYI RGATCY 3 cut(s) 94, 179, 535
Bsu15I ATCGAT 1 cut(s) 1357
BsuRI GGCC 3 cut(s) 34, 122, 1124
BsuTUI ATCGAT 1 cut(s) 1357
BtgI CCRYGG 1 cut(s) 29
BtgZI GCGATG 1 cut(s) 1323
BtsCI GGATG 6 cut(s) 477, 736, 772, 989, 1080, 1264
BtsI GCAGTG 2 cut(s) 742, 1005
BtsIMutI CAGTG 5 cut(s) 429, 573, 742, 1005, 1248
BveI ACCTGC 1 cut(s) 735
Cac8I GCNNGC 4 cut(s) 115, 878, 882, 1029
CaiI CAGNNNCTG 3 cut(s) 617, 1019, 1247
CfoI GCGC 1 cut(s) 221
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 4 cut(s) 120, 125, 133, 497
ClaI ATCGAT 1 cut(s) 1357
CseI GACGC 1 cut(s) 262
Csp6I GTAC 1 cut(s) 55
CviAII CATG 2 cut(s) 887, 1318
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 8 cut(s) 524, 549, 603, 662, 795, 996, 1073, 1172
DpnI GATC 9 cut(s) 96, 181, 353, 537, 577, 907, 1161, 1356, 1360
DpnII GATC 9 cut(s) 94, 179, 351, 535, 575, 905, 1159, 1354, 1358
DriI GACNNNNNGTC 1 cut(s) 796
EaeI YGGCCR 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 825, 1058
Eam1105I GACNNNNNGTC 1 cut(s) 796
EarI CTCTTC 2 cut(s) 825, 1058
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 183, 344
Eco147I AGGCCT 1 cut(s) 1124
Eco24I GRGCYC 1 cut(s) 209
Eco31I GGTCTC 1 cut(s) 1092
Eco47I GGWCC 3 cut(s) 125, 133, 497
Eco53kI GAGCTC 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 1196
EcoICRI GAGCTC 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 2 cut(s) 183, 344
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 2 cut(s) 183, 344
FaeI CATG 2 cut(s) 890, 1321
FaiI YATR 8 cut(s) 518, 690, 777, 873, 888, 897, 923, 1319
FaqI GGGAC 2 cut(s) 247, 299
FatI CATG 2 cut(s) 886, 1317
FauI CCCGC 3 cut(s) 96, 110, 1032
Fnu4HI GCNGC 4 cut(s) 35, 77, 504, 1169
FokI GGATG 6 cut(s) 464, 743, 779, 996, 1067, 1271
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 4 cut(s) 35, 77, 504, 1169
FspBI CTAG 1 cut(s) 756
GlaI GCGC 1 cut(s) 220
GluI GCNGC 4 cut(s) 35, 77, 504, 1169
GsaI CCCAGC 1 cut(s) 94
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 3 cut(s) 34, 122, 1124
HapII CCGG 2 cut(s) 123, 1084
HgaI GACGC 1 cut(s) 262
HhaI GCGC 1 cut(s) 221
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 2 cut(s) 890, 1321
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HindIII AAGCTT 1 cut(s) 1322
HinfI GANTC 8 cut(s) 379, 791, 797, 824, 845, 927, 1001, 1220
HpaII CCGG 2 cut(s) 123, 1084
Hpy166II GTNNAC 5 cut(s) 128, 156, 427, 955, 1005
Hpy188I TCNGA 9 cut(s) 42, 448, 651, 796, 829, 976, 1021, 1180, 1249
Hpy188III TCNNGA 2 cut(s) 229, 551
Hpy8I GTNNAC 5 cut(s) 128, 156, 427, 955, 1005
Hpy99I CGWCG 5 cut(s) 49, 219, 227, 278, 1167
HpyAV CCTTC 7 cut(s) 169, 454, 493, 812, 932, 1114, 1130
HpyCH4III ACNGT 3 cut(s) 30, 364, 619
HpyCH4V TGCA 5 cut(s) 113, 309, 890, 1010, 1031
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 73, 119, 653, 704, 741, 896
HpyF3I CTNAG 8 cut(s) 524, 549, 603, 662, 795, 996, 1073, 1172
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 2 cut(s) 890, 1321
HspAI GCGC 1 cut(s) 219
Kzo9I GATC 9 cut(s) 94, 179, 351, 535, 575, 905, 1159, 1354, 1358
LmnI GCTCC 3 cut(s) 406, 463, 1285
Lsp1109I GCAGC 2 cut(s) 490, 1180
LweI GCATC 2 cut(s) 428, 974
MaeI CTAG 1 cut(s) 756
MaeIII GTNAC 3 cut(s) 471, 798, 1297
MalI GATC 9 cut(s) 96, 181, 353, 537, 577, 907, 1161, 1356, 1360
MboI GATC 9 cut(s) 94, 179, 351, 535, 575, 905, 1159, 1354, 1358
MboII GAAGA 6 cut(s) 22, 61, 842, 854, 1075, 1145
MflI RGATCY 3 cut(s) 94, 179, 535
MhlI GDGCHC 2 cut(s) 209, 1290
MluCI AATT 9 cut(s) 555, 579, 675, 778, 892, 909, 1185, 1250, 1264
MlyI GAGTC 3 cut(s) 785, 806, 1010
MmeI TCCRAC 2 cut(s) 63, 391
MroXI GAANNNNTTC 1 cut(s) 863
MseI TTAA 1 cut(s) 1115
MspI CCGG 2 cut(s) 123, 1084
MspR9I CCNGG 1 cut(s) 283
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 2 cut(s) 219, 1308
MwoI GCNNNNNNNGC 7 cut(s) 31, 73, 119, 653, 704, 741, 896
NdeII GATC 9 cut(s) 94, 179, 351, 535, 575, 905, 1159, 1354, 1358
NlaIII CATG 2 cut(s) 890, 1321
NlaIV GGNNCC 4 cut(s) 121, 181, 388, 402
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 2 cut(s) 798, 1297
PaqCI CACCTGC 1 cut(s) 735
PceI AGGCCT 1 cut(s) 1124
PdmI GAANNNNTTC 1 cut(s) 863
PfeI GAWTC 5 cut(s) 379, 824, 845, 927, 1220
PflFI GACNNNGTC 1 cut(s) 272
PkrI GCNGC 4 cut(s) 36, 78, 505, 1170
PleI GAGTC 3 cut(s) 785, 805, 1009
PpsI GAGTC 3 cut(s) 785, 805, 1009
PsiI TTATAA 1 cut(s) 777
Psp124BI GAGCTC 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 281
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 4 cut(s) 121, 181, 388, 402
PspPI GGNCC 4 cut(s) 120, 125, 133, 497
PstNI CAGNNNCTG 3 cut(s) 617, 1019, 1247
PsuI RGATCY 3 cut(s) 94, 179, 535
PsyI GACNNNGTC 1 cut(s) 272
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 209
SaqAI TTAA 1 cut(s) 1115
SatI GCNGC 4 cut(s) 35, 77, 504, 1169
Sau3AI GATC 9 cut(s) 94, 179, 351, 535, 575, 905, 1159, 1354, 1358
Sau96I GGNCC 4 cut(s) 120, 125, 133, 497
SchI GAGTC 3 cut(s) 785, 806, 1010
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 2 cut(s) 209, 1290
SfaNI GCATC 2 cut(s) 428, 974
SfcI CTRYAG 1 cut(s) 958
SinI GGWCC 3 cut(s) 125, 133, 497
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
Sse9I AATT 9 cut(s) 555, 579, 675, 778, 892, 909, 1185, 1250, 1264
SseBI AGGCCT 1 cut(s) 1124
SsiI CCGC 8 cut(s) 35, 76, 79, 103, 117, 161, 1025, 1306
SspMI CTAG 1 cut(s) 756
SstI GAGCTC 1 cut(s) 209
StuI AGGCCT 1 cut(s) 1124
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 2 cut(s) 183, 344
TaaI ACNGT 3 cut(s) 30, 364, 619
TaqI TCGA 2 cut(s) 252, 1357
TaqII GACCGA 1 cut(s) 1224
TasI AATT 9 cut(s) 555, 579, 675, 778, 892, 909, 1185, 1250, 1264
TauI GCSGC 2 cut(s) 37, 79
TfiI GAWTC 5 cut(s) 379, 824, 845, 927, 1220
Tru1I TTAA 1 cut(s) 1115
Tru9I TTAA 1 cut(s) 1115
TscAI CASTG 5 cut(s) 429, 573, 742, 1012, 1248
TseFI GTSAC 2 cut(s) 798, 1297
TseI GCWGC 2 cut(s) 503, 1168
Tsp45I GTSAC 2 cut(s) 798, 1297
TspDTI ATGAA 5 cut(s) 951, 1069, 1197, 1212, 1334
TspGWI ACGGA 1 cut(s) 203
TspRI CASTG 5 cut(s) 429, 573, 742, 1012, 1248
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 3 cut(s) 125, 133, 497
XapI RAATTY 3 cut(s) 675, 1185, 1250
XmnI GAANNNNTTC 1 cut(s) 863
XspI CTAG 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.