pycom12g10530

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017284
Physical Location & Seq
Forward (+)
12553007 .. 12556254
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
ATGTTCGCCAAGCGGCTTTTGCAGAAAGCCATAAACAACTCTCAGCATAATTTGCCACGCGGGAGTTTGACGGCGGAAGACTTGGACCTGCGAGTTGCAGTTCACTATGGCATCCCATCCACGGCCTCCATTCTTGCTTTCGACCCGATTCAGCGCCTTTTGGCCATTGGAACATTGGATGGGAGGATTAAAGTAATCGGTGGTGATGGTATTGAAGGACTCTTTATATCCCCGAAGCAACTGCCTTACAAATACATTGAGTTTCTACAAAACCAGGGTTATTTGGTGAGCATCTTGAATGATAATGATATTCAGGTTTGGAATCTCGAGAGCAGGTCCCTAGTTTATTGTCTAGAATGGAAAACAAATATAACCGCTTTTTCTGTAATCCATGGCTCCAGTTTAATGTATGTTGGCGATGAGTATGCTCTAGTGGCTGTGGTGAAGTATGATGCAGAGGACGGAAAATTATTGCAATTGCCATATCATATTTCTGCAAATTCTTTAAATGAAGCTGCTGAGTTTCCATTTCCAACTGACCAACCCTGTTCGTCTGGAAATAGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000137 GO:0000139 GO:0000902 GO:0000904 GO:0002791 GO:0003674 GO:0005096 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005795 GO:0005802 GO:0005856 GO:0005886 GO:0005938 GO:0006810 GO:0006892 GO:0006893 GO:0006996 GO:0007010 GO:0007275 GO:0007399 GO:0007409 GO:0008047 GO:0008150 GO:0009653 GO:0009987 GO:0010008 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016192 GO:0017016 GO:0017137 GO:0017157 GO:0019899 GO:0019900 GO:0019901 GO:0022008 GO:0022607 GO:0030029 GO:0030030 GO:0030036 GO:0030154 GO:0030182 GO:0030234 GO:0030695 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0031090 GO:0031175 GO:0031267 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0031985 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048193 GO:0048468 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050708 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051046 GO:0051049 GO:0051179 GO:0051223 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0060627 GO:0061564 GO:0065003 GO:0065007 GO:0065009 GO:0070201 GO:0071840 GO:0071944 GO:0090087 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0098876 GO:0099568 GO:0120036 GO:0120039 GO:1903530
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 Synaptobrevin

Protein Analysis

190

Amino Acids

21.04

Weight (kDa)

5.3

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 43 - 130 5.5e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017284)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08370 FvH4_4g08370 FvH4_4g08370 FvH4_4g08370 FvH4_4g08370 FvH4_4g08370
pyrus_communis pycom12g10530 pycom12g16180
rosa_chinensis RchiOBHm_Chr6g0296391
rosa_laevigata RLG00000027043
rosa_multiflora Rmu_co8421439.1_g000001 Rmu_sc0011275.1_g000003
rosa_samantha Rh2BG113800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 96, 324
AccII CGCG 1 cut(s) 60
AciI CCGC 4 cut(s) 13, 60, 74, 375
AcoI YGGCCR 1 cut(s) 162
AcsI RAATTY 1 cut(s) 499
AfiI CCNNNNNNNGG 1 cut(s) 121
AgsI TTSAA 2 cut(s) 215, 298
AjnI CCWGG 1 cut(s) 273
AluBI AGCT 1 cut(s) 515
AluI AGCT 1 cut(s) 515
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 2 cut(s) 123, 162
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 1 cut(s) 499
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 2 cut(s) 85, 336
AsuHPI GGTGA 3 cut(s) 215, 298, 454
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 2 cut(s) 85, 336
BalI TGGCCA 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 502
BccI CCATC 3 cut(s) 124, 173, 200
BceAI ACGGC 2 cut(s) 87, 138
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 407, 441
BciT130I CCWGG 1 cut(s) 275
BfaI CTAG 3 cut(s) 341, 353, 431
BfoI RGCGCY 1 cut(s) 157
BfuAI ACCTGC 2 cut(s) 96, 324
BisI GCNGC 2 cut(s) 14, 516
BlsI GCNGC 2 cut(s) 15, 517
Bme1390I CCNGG 1 cut(s) 275
Bme18I GGWCC 2 cut(s) 85, 336
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 85, 336
BmiI GGNNCC 2 cut(s) 338, 397
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 3 cut(s) 120, 300, 442
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 382
BsaJI CCNNGG 3 cut(s) 120, 274, 391
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 3 cut(s) 120, 274, 391
BseGI GGATG 3 cut(s) 111, 116, 184
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMII CTCAG 2 cut(s) 56, 510
BseNI ACTGG 1 cut(s) 399
BseXI GCAGC 1 cut(s) 502
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 125, 164
BsiHKCI CYCGRG 1 cut(s) 326
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 2 cut(s) 125, 164
BsoBI CYCGRG 1 cut(s) 326
Bsp19I CCATGG 1 cut(s) 391
BspACI CCGC 4 cut(s) 13, 60, 74, 375
BspANI GGCC 2 cut(s) 125, 164
BspCNI CTCAG 2 cut(s) 55, 511
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 338, 397
BspMI ACCTGC 2 cut(s) 96, 324
BsrI ACTGG 1 cut(s) 399
BssECI CCNNGG 3 cut(s) 120, 274, 391
BssT1I CCWWGG 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 275
BstAPI GCANNNNNTGC 1 cut(s) 52
BstDEI CTNAG 2 cut(s) 42, 519
BstDSI CCRYGG 2 cut(s) 120, 391
BstF5I GGATG 3 cut(s) 111, 116, 184
BstFNI CGCG 1 cut(s) 60
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstMWI GCNNNNNNNGC 3 cut(s) 19, 52, 434
BstNI CCWGG 1 cut(s) 275
BstSCI CCNGG 1 cut(s) 273
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 502
BstV2I GAAGAC 1 cut(s) 84
BsuRI GGCC 2 cut(s) 125, 164
BtgI CCRYGG 2 cut(s) 120, 391
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 3 cut(s) 111, 116, 184
BveI ACCTGC 2 cut(s) 96, 324
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 2 cut(s) 85, 336
CviAII CATG 1 cut(s) 392
CviJI RGCY 7 cut(s) 16, 29, 125, 164, 396, 437, 515
CviKI_1 RGCY 7 cut(s) 16, 29, 125, 164, 396, 437, 515
DdeI CTNAG 2 cut(s) 42, 519
DraI TTTAAA 1 cut(s) 507
EaeI YGGCCR 1 cut(s) 162
EciI GGCGGA 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 391
Eco47I GGWCC 2 cut(s) 85, 336
Eco88I CYCGRG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 1 cut(s) 395
FaqI GGGAC 1 cut(s) 322
FatI CATG 1 cut(s) 391
FauI CCCGC 1 cut(s) 53
Fnu4HI GCNGC 2 cut(s) 14, 516
FokI GGATG 3 cut(s) 98, 103, 191
Fsp4HI GCNGC 2 cut(s) 14, 516
FspBI CTAG 3 cut(s) 341, 353, 431
GlaI GCGC 1 cut(s) 155
GluI GCNGC 2 cut(s) 14, 516
GsuI CTGGAG 1 cut(s) 382
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 2 cut(s) 125, 164
HhaI GCGC 1 cut(s) 156
Hin1II CATG 1 cut(s) 395
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HinfI GANTC 3 cut(s) 148, 219, 322
HphI GGTGA 3 cut(s) 215, 298, 454
Hpy166II GTNNAC 1 cut(s) 103
Hpy188III TCNNGA 5 cut(s) 295, 326, 328, 353, 555
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 1 cut(s) 209
HpyCH4V TGCA 5 cut(s) 22, 98, 455, 475, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 52, 434
HpyF3I CTNAG 2 cut(s) 42, 519
Hsp92II CATG 1 cut(s) 395
HspAI GCGC 1 cut(s) 154
LmnI GCTCC 1 cut(s) 401
LpnPI CCDG 8 cut(s) 101, 260, 287, 299, 319, 412, 540, 559
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 3 cut(s) 120, 300, 442
MaeI CTAG 3 cut(s) 341, 353, 431
MboII GAAGA 1 cut(s) 89
MfeI CAATTG 1 cut(s) 476
MlsI TGGCCA 1 cut(s) 164
MluCI AATT 4 cut(s) 49, 467, 476, 499
MluNI TGGCCA 1 cut(s) 164
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 1 cut(s) 557
MnlI CCTC 3 cut(s) 136, 177, 451
Mox20I TGGCCA 1 cut(s) 164
MscI TGGCCA 1 cut(s) 164
MseI TTAA 4 cut(s) 189, 404, 506, 568
Msp20I TGGCCA 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 275
MunI CAATTG 1 cut(s) 476
MvaI CCWGG 1 cut(s) 275
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 3 cut(s) 19, 52, 434
NcoI CCATGG 1 cut(s) 391
NlaIII CATG 1 cut(s) 395
NlaIV GGNNCC 2 cut(s) 338, 397
PaeR7I CTCGAG 1 cut(s) 326
PfeI GAWTC 2 cut(s) 148, 322
PkrI GCNGC 2 cut(s) 15, 517
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
PpuMI RGGWCCY 1 cut(s) 336
Psp5II RGGWCCY 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 2 cut(s) 338, 397
PspPI GGNCC 2 cut(s) 85, 336
PspPPI RGGWCCY 1 cut(s) 336
SaqAI TTAA 4 cut(s) 189, 404, 506, 568
SatI GCNGC 2 cut(s) 14, 516
Sau96I GGNCC 2 cut(s) 85, 336
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 1 cut(s) 275
SetI ASST 5 cut(s) 90, 318, 338, 517, 566
SfaNI GCATC 3 cut(s) 120, 300, 442
Sfr274I CTCGAG 1 cut(s) 326
SinI GGWCC 2 cut(s) 85, 336
SlaI CTCGAG 1 cut(s) 326
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 4 cut(s) 49, 467, 476, 499
SsiI CCGC 4 cut(s) 13, 60, 74, 375
SspMI CTAG 3 cut(s) 341, 353, 431
StyD4I CCNGG 1 cut(s) 273
StyI CCWWGG 1 cut(s) 391
TaqI TCGA 2 cut(s) 141, 327
TasI AATT 4 cut(s) 49, 467, 476, 499
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 2 cut(s) 148, 322
Tru1I TTAA 4 cut(s) 189, 404, 506, 568
Tru9I TTAA 4 cut(s) 189, 404, 506, 568
TseI GCWGC 1 cut(s) 515
TspDTI ATGAA 1 cut(s) 525
TspGWI ACGGA 1 cut(s) 477
VpaK11BI GGWCC 2 cut(s) 85, 336
XapI RAATTY 1 cut(s) 499
XbaI TCTAGA 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XhoI CTCGAG 1 cut(s) 326
XspI CTAG 3 cut(s) 341, 353, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.